Digital Commons Network^™

Molecular Dynamics Simulations Of Liposomes: Structure, Dynamics, And Applications, Ehsan Khodadadi, Ehsaneh Khodadadi, Parth Chaturvedi, Mahmoud Moradi

Chemistry & Biochemistry Faculty Publications and Presentations

Liposomes are nanoscale, spherical vesicles composed of phospholipid bilayers, typically ranging from 50 to 200 nm in diameter. Their unique ability to encapsulate both hydrophilic and hydrophobic molecules makes them powerful nanocarriers for drug delivery, diagnostics, and vaccine formulations. Several FDA-approved formulations such as Doxil (R) (Baxter Healthcare Corporation, Deerfield, IL, USA), AmBisome (R) (Gilead Sciences, Inc., Foster City, CA, USA), and Onivyde (R) (Ipsen Biopharmaceuticals, Inc., Basking Ridge, NJ, USA) highlight their clinical significance. This review provides a comprehensive synthesis of how molecular dynamics (MD) simulations, particularly coarse-grained (CG) and atomistic approaches, advance our understanding of liposomal membranes. We …

Genetic And Environmental Factors Contributing To The Pathogenesis Of Vertebral Osteomyelitis Caused By Enterococcus Cecorum In Broiler Chicken, Ummu Syauqah Al Musyahadah, Andi Asnayanti, Anh Dang Trieu Do, Adnan Alrubaye

Poultry Science Faculty Publications and Presentations

Enterococcus cecorum (EC) is the primary cause of Vertebral Osteomyelitis (VO) in poultry, characterized by a hock-sitting posture with legs extended forward. The disease begins with intestinal colonization, where pathogenic strains of E. cecorum establish themselves in the gut within the first week of a bird's lifecycle and translocate to the bloodstream later, reaching the organs, which finally infect the birds' Free Thoracic Vertebrae (FTV). Several factors contribute to the initiation and progression of the disease. Environmental factors such as heat stress, high blood sugar levels, prior infections, overcrowding, and rapid growth conditions weaken immunity and stimulate the emergence of …

Novel Nanomaterials For Developing Bone Scaffolds And Tissue Regeneration, Nazim Uddin Emon, Lu Zhang, Shelby Dawn Osborne, Mark Allen Lanoue, Yan Huang, Z. Ryan Tian

Cell and Molecular Biology Faculty Publications and Presentations

Nanotechnologies bring a rapid paradigm shift in hard and soft bone tissue regeneration (BTR) through unprecedented control over the nanoscale structures and chemistry of biocompatible materials to regenerate the intricate architecture and functional adaptability of bone. This review focuses on the transformative analyses and prospects of current and next-generation nanomaterials in designing bioactive bone scaffolds, emphasizing hierarchical architecture, mechanical resilience, and regenerative precision. Mainly, this review elucidated the innovative findings, new capabilities, unmet challenges, and possible future opportunities associated with biocompatible inorganic ceramics (e.g., phosphates, metallic oxides) and the United States Food and Drug Administration (USFDA) approved synthetic polymers, including …

Landscape Of Clinical Trials In Cancer Cachexia: Assessment Of Trends From 1995-2024, Ana Regina Cabrera, Kaitlyn Parker, Deena B. Snoke, Bart Hammig, Nicholas P. Greene

Health, Human Performance and Recreation Faculty Publications and Presentations

Background Cancer cachexia, a multifactorial syndrome characterized by unintentional weight loss, is a frequent complication of cancer that impacts patients' quality of life and survival.

Methods In this retrospective review, we evaluated the landscape of clinical trials registered on ClinicalTrials.gov for the consideration of potential factors contributing to biological human heterogeneity in their design and analyses.

Results Among clinical trials registered from 1995-2024, we observed increased inclusion of female participants, but lack of reporting of sex as a biological variable. The majority (similar to 93%) of participants were of Caucasian descent. There was a substantial divergence in the diagnostic criteria …

Perceived Obligations Of Older Partners In Living-Apart Together Relationships, Allison M. Martin

Human Development and Family Sciences Undergraduate Honors Theses

Older and middle-aged adults are increasingly in Living-Apart-Together (LAT) relationships characterized by committed romantic relationships without marriage and cohabitation. Because of assumptions that older adults will receive family caregiving as they grow older and need caregiving, it is not clear how people are likely to perceive obligations to provide caregiving assistance. As adults move into old age and incidence rates of Alzheimer’s Disease or Related Dementia (ADRD) increase, questions about the availability of family members to provide needed assistance are complicated by LAT relationships. The purpose of this study was to examine perceived obligations to provide assistance to older adults …

Co-Creating Play:Utilizing Comics To Potentialize Participatory Self-Determination In Marginilized Young Adults, Marlin Mcknight

Graduate Theses and Dissertations

Storytelling has long served as a vital mechanism for preserving cultural heritage, transmitting values, and fostering intergenerational connection (Moeshlin, 2024). Despite this significance, graphic narratives such as comics and graphic novels have been historically marginalized—dismissed as trivial “children’s media” or reduced to entertainment rather than recognized as culturally resonant tools (McCloud, 1993). This marginalization intensified as comics evolved to depict adult themes and, at times, harmful stereotypes, triggering social backlash that stigmatized their utility for younger audiences (Segran, 2020). Consequently, scholarly exploration of comics’ therapeutic potential has stagnated, hindered by the medium’s tumultuous history. This study bridges this gap by …

In Vitro Assessment Of The Antihistomonal Activity Of Methyl Jasmonate And Jasmonic Acid, Mckayla B. Williams

Poultry Science Undergraduate Honors Theses

Histomonas meleagridis is a protozoan parasite and the causative agent of histomonosis in gallinaceous birds. There are currently no approved prevention or treatment options available for histomonosis control in the United States. Mortality in affected turkey flocks can exceed 80% which highlights the urgent need for research investigating novel strategies to mitigate histomonosis in the field. The objective of this study was to evaluate the in vitro antihistomonal and antibacterial effects of of methyl jasmonate (MeJA) and jasmonic acid (JA), two plant-derived compounds, in a xenic culture of H. meleagridis. Assays were conducted using cultured H. meleagridis (PHL strain) treated …

Design, Modeling, Control, And Analysis Of A Modular Two-Wheeled Self-Balancing Robotic System, Mishek Jair Musa

Graduate Theses and Dissertations

Two-wheeled self-balancing robots have garnered substantial attention within the realms of research and innovation in academia and industrial settings. In particular, advances in control algorithms, machine learning, reinforcement learning, and sensor technologies have played a pivotal role in their development. Although primarily recognized for their utility in personal transportation in the commercial sector, these robots possess potential applications across various domains, including search and rescue, healthcare, material handling, logistics, etc., due to their active stabilization and exceptional maneuvering capabilities. A particular area of interest lies in the creation of modular self-balancing robots designed for seamless reconfiguration and enhanced human interaction. …

Modeling U.S. Corn Production Archetypes In The Agricultural Policy/Environmental Extender Model To Generate Life Cycle Assessment Inventory Data, Brandon Taylor

Graduate Theses and Dissertations

Corn (Zea mays) is an important crop grown across the US for many uses such as grain for animal feed, bio-ethanol production, food products, and industrial products. Corn is produced in almost every state in the US, but most corn production is concentrated in the Corn Belt Region including Iowa, Illinois, Ohio, Indiana, Minnesota, and Nebraska. Although technologies and production practices are improving corn production efficiency primarily through increased yields (improved land use), corn production at this scale greatly impacts the environment through emissions from fertilizer and pesticide runoff and leaching, soil degradation due to improper field practices, as well …

Multivariate Optical Imaging Of The Oxygen-Metabolism Axis, Jesse D. Ivers

Graduate Theses and Dissertations

Metabolic reprogramming is a characteristic hallmark of multiple pathologies and promotes disease progression, survival, and severity. Current methods to investigate metabolic reprogramming, however, require reductions of dimensionality both because of instrumentation limitations and analysis tools. This work aims to define a blueprint for high dimensional study of the oxygen metabolism axis to enable more sensitive study metabolic reprogramming. We hypothesized (1) multivariate imaging of metabolic and oxygenation metrics in ex vivo tissue sections would elucidate differences in resistant and sensitive head and neck squamous cell carcinoma (HNSCC) xenografts that traditional uni-variate methods miss and (2) an engineered hyperspectral darkfield microscopy …

Controlling Electroless Deposition On Polymer Interfaces Towards Discrete Functional Particle Synthesis, Grecia Pierina Alvarado Munoz

Graduate Theses and Dissertations

Electroless deposition is a process that facilitates the formation of metal layers on surfaces through the reduction of metal ions onto a surface in the presence of a sacrificial reducing agent. This process can be applied to synthesize conductive metal layers on insulators such as glass or polymers. The kinetics of heterogeneous deposition depend on the interfacial surface chemistry, including the presence of added catalyst materials, as well as the bulk chemical environment, such as the concentration of metal ions and sacrificial reductants. Furthermore, the homogeneous formation of metal nanoparticles may compete with the deposition reaction, adversely affecting the control …

Synthesis And Characterization Of Polymer-Coated Metal Nanostructures And Gelatin-Incorporated Hydrogels For Biomedical Use, Deborah Takyibea Okyere

Graduate Theses and Dissertations

The development of advanced materials for disease management and treatment presents a pressing challenge in the health sector. The overall objective of this research is to contribute to the development of advanced materials with tailored properties for biomedical applications, with a focus on cost-effective and robust nanoparticles and biomaterials. The first part of this work focuses on designing and synthesizing earth-abundant copper nanoparticles with desired optical properties. Copper nanoparticles, which provide a more affordable alternative to gold and silver, are being investigated due to their unique plasmonic properties. However, their susceptibility to oxidation limits their application. To address this challenge, …

A Multicomponent Microneedle Patch For The Delivery Of Meloxicam For Veterinary Applications, Katherine Alejandra Miranda Munoz

Graduate Theses and Dissertations

Veterinary medicine requires innovative drug delivery strategies to enhance therapeutic efficacy while minimizing stress and handling. This dissertation explores the development and application of biodegradable microneedle patches for the transdermal delivery of meloxicam in livestock, offering a minimally invasive alternative to traditional administration methods. The research investigates the fabrication and characterization of microneedles using polyvinyl alcohol, collagen, and chitosan blends, demonstrating their mechanical strength, biocompatibility, and ability to facilitate sustained drug release. In vitro and in vivo evaluations confirm effective skin penetration, dissolution, and localized drug delivery. Pharmacokinetic studies highlight anatomical site-dependent variations in drug absorption, emphasizing the need for …

Advancing Point-Of-Care Diagnosis Of Joint Infection Using A Diffuse Reflectance Spectroscopy (Drs) System, Erin Elizabeth Drewke

Graduate Theses and Dissertations

Septic arthritis is a severe joint infection that can rapidly destroy cartilage and cause lifelong disability if not diagnosed and treated promptly. Faster, bedside diagnostic tools are needed to reduce delays and support timely treatment decisions while preserving standard laboratory workflows. A syringe-mounted diffuse reflectance spectroscopy (DRS) system was developed for rapid, sterile, point-of-care measurement of synovial fluid optical properties. Broadband light (400–650 nm) was delivered and collected through the syringe barrel without contaminating the sample. An empirical lookup table (LUT) and a boundary-aware Monte Carlo (MC) model were implemented to extract absorption (μₐ) and reduced scattering (μs′) coefficients from …

Effects Of Flavonoids On Reducing Colon Cancer Risk Via Macrophage Polarization, Gracien Jules

Graduate Theses and Dissertations

Colorectal cancer is one of the most fatal diseases in the United States. Chronic tissue inflammation can cause cell proliferation, which leads to tumor formation and cancer development. Macrophages are responsible for maintaining tissue homeostasis and for inflammatory responses. M1 macrophages exhibit proinflammatory responses while M2 macrophages exhibit anti-inflammatory responses. Imbalance between M1 and M2 macrophages is closely related to inflammation-associated disease development. The anti-inflammatory effects of apiaceous vegetable constituents, specifically flavonoids, have been studied for their preventative effects towards reducing disease risk. However, sufficient evidence is lacking on the mechanisms by which flavonoids impact M1/M2 macrophage polarization. RAW 264.7 …

Testing A Culturally Grounded Training For Black And Latine Clinicians: Creating Communities In Pcit For Providers Of Autistic Youth, Harlee Onovbiona

Graduate Theses and Dissertations

Black and Latine families of autistic youth continue to face mental health disparities, some of which have been attributed to poor access to services, discrimination, and poor culturally responsive care. Black and Latine families call for more family-centered treatments that are in accordance with their values and culture with options for treatments to be delivered by clinicians with similar racial and ethnic backgrounds. Parent-Child Interaction Therapy (PCIT) is a widely known evidenced-based treatment (EBT) that has been used with Latine, Black, and neurodiverse children to improve the parent-child relationship and reduce challenging behaviors. Although considerable efforts have been made to …

Utilizing The Horseshoe Prior In Exploratory Factor Analysis And Gaussian Graphical Networks, James Thomas Roddy

Graduate Theses and Dissertations

High-dimensional data analysis frequently involves extracting meaningful structure from noisy, sparse signals. In recent years, Bayesian shrinkage priors—particularly global-local shrinkage priors—have emerged as powerful tools for inducing sparsity while preserving signal fidelity. Among these, the Horseshoe prior has gained notable attention for its capacity to simultaneously shrink irrelevant parameters and retain substantial signals. This dissertation explores the Horseshoe prior as a unified framework for sparse Bayesian inference across theory, simulation, and real-world application. The first component develops new theoretical results establishing the asymptotic Bayes optimality of the Horseshoe prior in Gaussian graphical models (GGMs). We consider sparse precision matrix estimation …

From Stem Cells To Smooth Muscle Cells: Characterizing Secretome Profiles And Evaluating Regenerative Potentials, Christofer Baldwin

Graduate Theses and Dissertations

This dissertation examines the role of vascular smooth muscle cells (VSMCs) in vascular disease and the potential of stem cell-derived VSMCs (SC-SMCs) for regenerative medicine. VSMCs are crucial for maintaining function; however, their dysregulation contributes to the progression of diseases like atherosclerosis, vascular calcification, and aneurysms. Disease progression is influenced by various environmental factors, prompting VSMCs to switch phenotypes, leading to adverse vascular remodeling. This research focuses on adult bone marrow mesenchymal stem cells (BM-MSCs) and adipose-derived stem cells (ADSCs) as sources for generating reparative VSMCs. Through comparative analysis, it investigates the effects of SC-SMCs on VSMC biomarker expression and …

Small Angle X-Ray Scattering Studies Of Collagenase H Mutant, Colh-E416q, Madelyn Millsap

Graduate Theses and Dissertations

Collagenase H (ColH) from Hathewaya histolytica plays a central role in collagen degradation through endopeptidase and carboxytripeptidase reactions. To better understand how its domain architecture supports these functions, we examined the solution structure of a catalytically inactive mutant, ColH-E416Q, using small-angle X-ray scattering (SAXS). The E416Q mutant is catalytically inactive because the glutamate residue at position 416, which normally functions as a general acid/base during catalysis, is replaced by glutamine—an amide that cannot donate or accept protons effectively, thus disabling the proton transfer steps essential for peptide bond hydrolysis. SAXS results indicate that the E416Q substitution leaves overall domain architecture …

Effect Of Marination And Storage Time On The Properties And Meat Quality Of Broiler Breast, Mayra Mabel Herrera Rivera

Graduate Theses and Dissertations

Abstract The poultry industry has implemented numerous methods, as marination and aging, to improve meat quality and satisfy consumer demands. Three experiments were developed to assess the effects of vacuum tumble marination and aging on the proteins of chicken breast. These experiments utilized 0.75 % sodium chloride, 0.45 % phosphate, and water in a marinade. In experiment one, 270 fillets were allocated into six treatment groups, each comprising 45 fillets. Six days of storage time were used, marinating and storing the fillets in each group at a temperature of 4°C. Following each day of storage period, the fillets underwent a …

Analysis Of The Scleroderma / Systemic Sclerosis Etiopathology Using The University Of California At Davis (Ucd)-Ssc Chicken Line, Angeline E. Rodriguez

Graduate Theses and Dissertations

Scleroderma/Systemic Sclerosis (SSc) is a multifaceted autoimmune disorder affecting approximately 300,000 people in the United States. Main features include vasculitis, immune dysfunction (innate and adaptive), autoantibody production, perivascular leukocyte infiltration, and extensive tissue fibrosis. The University of California, Davis (UCD) SSc chicken line was shown to exhibit all clinical and biological manifestations of human SSc. One early and distinctive clinical sign in both humans and UCD-SSc chickens is Raynaud’s phenomenon (RP), characterized by decreased blood flow in extremities and skin lesions. RP-like lesions develop in combs of UCD-SSc chickens within one- to two-weeks post-hatch, progressing from erythema and edema to …

Host-Virus Interaction Dynamics Using Sulfolobus Spindle-Shaped Virus And Host Sulfolobales System, Yeasin Ahmed

Graduate Theses and Dissertations

The Sulfolobus Spindle-shaped virus (SSV) is among the most studied archaeal viruses, particularly in extreme environments. This virus predominantly infects multiple Crenarchaea from the Sulfolobaceae family. These hosts thrive in extreme environmental conditions, such as sulfuric hot springs, with a pH of 2 to 4 and a temperature of 76-80˚C. Sulfolobales exhibit a wide geographical distribution across volcanic hot springs. The Sulfolobus Spindle-shaped Virus (SSV), encompassing strains such as SSV1 and SSV8, employs a non-lytic replication strategy in hosts belonging to the order Sulfolobales. The SSV-Sulfolobales system can be cultured in liquid and gellan gum solid media. A comprehensive study …

Predicting Case Clearance For Victims Of Anti-Transgender Homicide In The United States, Brittaney Alyssa Mann

Graduate Theses and Dissertations

There is a shortage of research exploring case clearance of anti-transgender bias homicides. This study will comparatively examine the similarities and differences in anti-transgender bias homicide cases with and without arrests at the victim, situational and incident levels occurring in the United States between 2013 and 2023. Data are extracted from the Bias Homicide Database (BHDB), the National Coalition of Anti-Violence Programs (NCAVP), and the Human Rights Campaign (HRC). This project will address the gap in understanding case clearance of anti-transgender bias homicides, provide policy implications, and ideas to further the discipline’s understanding of the topic. Keywords: LGBTQIA+ victims, bias …

Polymer-Enhanced Nanopore Sensing, Eugene Gyasi Agyemang

Graduate Theses and Dissertations

The resistive pulse technique characterizes single analytes by detecting fluctuations in the ion current as they pass through a narrow orifice connecting two electrolyte-filled chambers, each containing a single electrode. The amplitude, shape, frequency, and duration of these fluctuations primarily provide information about the analyte’s size, shape, concentration, and net surface charge, respectively. One configuration for resistive pulse measurements is of an electrolyte-filled nanopipette immersed in a bath, where the nanopipette tip acts as the nanoscale orifice. It was previously shown that incorporating the large quantities polymer polyethylene glycol (PEG 8K, 50% w/v) into the external electrolyte bath dramatically enhanced …

A Grounded Theory Investigation Of The Experiences Of Rehabilitation Administrators, Counselors, And Supervisors Providing Services To Women With Disabilities Experiencing Intimate Partner Violence, Dana Posey

Graduate Theses and Dissertations

Abstract There is a noticeable gap in the literature as it relates to how rehabilitation and disability service providers have worked with women with disabilities who have experienced intimate partner violence. To address the dearth of knowledge, a qualitative grounded theory study was conducted with 12 rehabilitation administrators, counselors, and supervisors regarding their experiences providing services to women with disabilities who have been in or are in an intimate partner violence relationship. Semi-structured interviews were administered, recorded, and transcribed verbatim in an ongoing manner throughout the process. As data was collected, it was analyzed using NVIVO 15, which is a …

Impact Of Three Professional Development Models (Online, Face-To-Face, And Hybrid) On Bhutanese Inservice Science Teachers' Conceptions Of Aspects Of Nature Of Science (Nos) With Reports Of Translation Of Nos Content Into Classroom Teaching Practices, Pabi Maya Das

Graduate Theses and Dissertations

Having a scientifically literate citizenry is an asset nationally and globally, and knowledge of the nature of science (NOS) is a crucial element in such literacy. Science teachers are a vital link if students are to understand key elements of the NOS, yet teachers themselves often lack knowledge of NOS and how to integrate NOS content into science lessons. Investigating the impact of a series of inservice professional development experiences using three distinct delivery modes (face-to-face, hybrid, online) for teachers in my home country of Bhutan is the overarching goal of the study reported here. The study used a mixed-methods …

Lifetime Stressor Exposure, Race And Ethnicity, And Habitual Stress Processes: An Evaluation Of Repetitive Negative Thinking And Hair Cortisol Concentration, Harrison Dickens

Graduate Theses and Dissertations

Habitual stress processes are thought to influence acute stress reactivity and stress-related disease. Habitual stress processes have been shown to adapt in response to stressor exposure experienced across the lifespan. The current study seeks to explore whether two habitual stress processes, hypothalamic-pituitary-adrenal (HPA) axis activity and repetitive negative thinking (RNT), were associated with lifetime stressor exposure (self-reported and objective neighborhood-level stressors). Additionally, the current study attempts to quantify how this process may be moderated by race and ethnicity. Participants (N = 134, Mage = 18 [SDage= 1.69], 56.7% female) consisting of White (N = 52), Black (N = 37 ), …

Improved Autofluorescence Lifetime Decay Analysis For Identification Of Cellular Autofluorescence Biomarkers For Label-Free, Single-Cell Assessment Of Mitochondrial Membrane Potential, Olivia Isabel Kolenc

Graduate Theses and Dissertations

Optical imaging of metabolism using the autofluorescence of the metabolic cofactors NADH, NADPH, and FAD has a wide range of sensitivities and the capability to collect a great deal of metabolic information. Multiphoton microscopy (MPM) is a non-invasive, label-free optical imaging technique capable of performing fluorescence lifetime imaging (FLIM) using time correlated single photon counting (TCSPC). However, autofluorescence FLIM presents a challenge in collecting sufficient photons to provide adequate signal to noise ratios (SNR) for analysis. Additionally, the specificity of autofluorescence biomarkers of metabolism has not been well-defined. Contextualizing these autofluorescence measures using established functional biomarkers can improve interpretability of …

Crystal Structures Of Escherichia Coli Glucokinase And Insights Into Phosphate Binding, Joseph Andrews, Joshua Sakon, Chenguang Fan

Chemistry & Biochemistry Faculty Publications and Presentations

Here, we report the crystal structure of Escherichia coli glucokinase (GLK), which has phosphate bound in the cleft between the alpha and beta domains adjacent to the active site. A ternary complex consisting of GLK, glucose and phosphate is also reported in this work. Diffraction data were collected at 2.63 angstrom resolution for the phospate-bound form (R-work/R-free = 0.191/0.230) and at 2.54 angstrom resolution for the ternary complex (R-work/R-free = 0.202/0.258), both at 297 K. A B-factor analysis of the phosphate-bound GLK structure revealed consistently lower values for phosphate-interacting basic residues in the alpha 4, alpha 5 and alpha 9 …

The Upcoming Moral Crisis In Primitive Artificial Intelligence, Justin E. Edens

Graduate Theses and Dissertations

As we continue to develop artificially intelligent systems, there is an increasingly high chance that we will develop a system that is both conscious and capable of suffering. Furthermore, it is likely that the development of this conscious machine will be entirely unintentional. While this machine will have moral status, identifying it will be extremely difficult, leading to it being treated the same as its inert predecessors. For these reasons I believe that a crisis in ethics is looming. This paper aims to argue that it is possible for a machine to have moral status, that the first such machine …