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Single Molecule Fluorescence Studies Of Protein Structure And Dynamics Underlying The Chloroplast Signal Recognition Particle Targeting Pathway, Dustin R. Baucom

Graduate Theses and Dissertations

The work presented in this dissertation explores the structural dynamics in the chloroplast signal recognition particle pathway. Findings include cpSRP shows scanning functionality similar to that in the cytosolic SRP with the ribosome. The intrinsically disordered C-terminal tail of the Albino3 protein has some transient secondary structure. Upon binding to cpSRP43 in solution, separate secondary structure formation was identified in the C-terminal tail of Albino3. Finally, to increase efficiency of analyzing fluorescence time traces for this work, a modular software was produced.

Influence Of Single And Multiple Histidine Residues And Their Ionization Properties On Transmembrane Helix Dynamics, Orientations And Fraying, Fahmida Afrose

Graduate Theses and Dissertations

Since aromatic and charged residues are often present in various locations of transmembrane helices of integral membrane proteins, their impacts on the molecular properties of transmembrane proteins and their interactions with lipids are of particular interest in many studies. In this work, I used solid-state deuterium NMR spectroscopy in designed model peptide GWALP23 [GGALW(LA)6LWLAGA] with selective deuterium labels to addresses the pH dependence and influence of single and multiple “guest” histidine residues in the orientation and dynamic behaviors of transmembrane proteins. The mutations include Gly to His (G2/22 to H2/22), Trp to His (W5/19 to H5/19) and Leu to His …

Investigating Egg Storage Conditions, Hatch Characteristics, And Feeding Methods Of Commercially Produced Poultry, Joshua R. Deines

Graduate Theses and Dissertations

Advancements in poultry research continue to add knowledge and understanding to an already technical industry. Such information has led to new developments in early feeding strategies which provide nutrients to broiler chicks while in the egg or during transport. This wealth of knowledge in poultry research extends beyond chickens and is utilized for other species, such as quail and pheasants, including how fertile eggs are stored and incubated. However, methods of early feeding broilers have not been investigated in industry relevant approaches to understand its effects, including how timing of feed access interacts with the hatch window. As well, quail …

Nematode Populations As Affected By Residue And Water Management In A Long-Term Wheat-Soybean Double Crop In Eastern Arkansas, Lucia Emperatriz Escalante Ortiz

Graduate Theses and Dissertations

Soybeans (Glycine max) are one of the major row crops in the United States, particularly in Arkansas. Soybean cyst nematode (SCN, Heterodera glycines) and southern root-knot nematode (RKN, Meloidogyne incognita) are two of the most damaging pests that cause major economic losses in soybeans. Little is known concerning the effects of common and alternative agronomic practices on nematodes in fields with nematode population densities below threshold levels. Therefore, the objective of this study was to evaluate the effects of the combination of tillage (conventional tillage and no-tillage), irrigation (irrigated and non-irrigated), wheat (Triticum aestivum) residue burning (burned and no burned), …

Analyzing Multigene Stacking And Genome Editing Strategies In Rice, Bhuvan Pathak

Graduate Theses and Dissertations

Crop improvement through biotechnology is an integrated effort, incorporating multiple approaches like integration of genes, editing of native genes, and removal of selection marker genes. Before streamlining the protocols, the efficiency and feasibility of the individual approach and their components must be tested. This study evaluated following approaches: 1) stacking an array of genes into a single locus by site-specific integration via Cre-lox recombination in rice, 2) determining the efficiency of I-SceI and the CCR5-ZFN in the targeted excisions of gene fragments in rice and Arabidopsis, and 3) determining the efficiency of CRISPR/Cas9 in generating targeted mutations for genome editing …

Evaluation Of Topramezone For Use In Rice (Oryza Sativa L.) Production, Matthew Moore

Graduate Theses and Dissertations

The rapid development of resistant weeds, particularly to postemergence-applied herbicides, is a growing concern to rice producers worldwide. Barnyardgrass, one of the most problematic weeds in rice cropping systems, alone has been found to be resistant to four herbicide sites of action (SOA) in certain populations, leaving growers no options for control. This unsettling fact has led to research on other SOAs that have not previously been used in US rice. One SOA that has garnered interest is the 4-hydroxyphenylpyruvate dioxygenase (HPPD)- inhibiting herbicides. The only HPPD-inhibitor that will soon be labeled for use in US rice is benzobicyclon. The …

Extracting Social Network From Literary Prose, Tarana Tasmin Bipasha

Graduate Theses and Dissertations

This thesis develops an approach to extract social networks from literary prose, namely, Jane Austen’s published novels from eighteenth- and nineteenth- century. Dialogue interaction plays a key role while we derive the networks, thus our technique relies upon our ability to determine when two characters are in conversation. Our process involves encoding plain literary text into the Text Encoding Initiative’s (TEI) XML format, character name identification, conversation and co-occurrence detection, and social network construction. Previous work in social network construction for literature have focused on drama, specifically manually TEI-encoded Shakespearean plays in which character interactions are much easier to track …

Women In Stem: Strategies And Recommendations For Academic Women And Institutional Leaders, Sarah Jensen

Graduate Theses and Dissertations

Women still comprise a small number of full professors in STEM disciplines in research universities, which have historically been male dominated. The National Science Foundation (NSF) has recognized the challenge of getting more women to enter the professoriate, earn tenure, and advance to full professor. These women can then encourage more women to enter into STEM as educators and researchers. The purpose of this study was to recognize, explore, and depict strategies used by women full professors to overcome obstacles they faced while advancing in academic STEM fields. The study participants also offered recommendations for women faculty desiring to become …

Death On The Horizon: Osteoethnography Of The People Of Akhetaten, Alissa Michelle Bandy

Graduate Theses and Dissertations

The purpose of this dissertation is to define and implement osteoethnography. Osteoethnography is the analysis and description of an ancient culture through the bioarchaeological and archaeological evidence, utilizing cultural anthropological theories and techniques. An osteoethnographic narrative is presented in this dissertation, which describes the embodied lives of the people of the 18th Dynasty Egyptian city of Akhetaten, now known as Amarna, founded in 1355 B.C.E. by the Pharaoh Akhenaten. Osteoethnography looks at how people are shaped by and shape their environment, how culture impacts health, and how culture informs the lives of its practioners. Osteoethnography employs life course theory, and …

Genetic Resistance To The Downy Mildew Pathogen And Mapping The Rpf Resistance Loci In Spinach, Gehendra Bhattarai

Graduate Theses and Dissertations

Spinach (Spinacia oleracea) is an important cool-season leafy vegetable crop in the United States (US). Downy mildew, caused by the obligate oomycete Peronospora effusa, is the most economically important disease of spinach. A total of 17 races of P. effusa have been reported on spinach, and many of these races (>10) have emerged in the last three decades. The new races of the pathogen are continually overcoming the genetic resistances used in the newly released cultivars.

A detached leaf inoculation assay was evaluated, standardized, and validated as a new method to differentiate resistant and susceptible spinach genotypes. Disease response …

Quantification Of Palmer Amaranth Seed Number Using A Computerized Particle Analyzer, Matthew B. Bertucci, Paul C. Bartley Iii, Katherine M. Jennings, David W. Monks, Brian E. Jackson

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

We evaluated the accuracy of a computerized particle analyzer (CPA) for high-throughput counting of Palmer amaranth (Amaranthus palmeri S. Watson) seeds and subsequently used the CPA to verify the accuracy of two subsampling methods for estimation of Palmer amaranth seed production. To determine accuracy of the CPA, 55 hand-counted samples, ranging from 500 to 5000 Palmer amaranth seeds, were drawn from field samples and counted with the CPA. The relationship between hand and CPA seed counts was described by a linear model (R-2 = 0.99) with a slope of 0.987 and a y-intercept of 3.49. Thus, very little discrepancy exists …

Hvac System Energy Audit For Leverett Elementary School, Connor Smalling

Biological and Agricultural Engineering Undergraduate Honors Theses

Leverett Elementary School is located in Fayetteville, AR. The school needs significant upgrades to its infrastructure. The Fayetteville Public School District has voted to pursue an Energy Services Performance Contract (ESPC) in order to finance the desired upgrades to Leverett Elementary, among other schools in the district.

The scope of this thesis was to perform an energy audit on the existing heating, ventilation, and air conditioning (HVAC) system. By using an energy modeling software, eQuest, the building and the existing base system were modeled to determine utility consumption. Three different HVAC system alternatives were analyzed against the base system by …

Drivers Of On-Farm Performance Of Irrigation Water Management Practices: Empirical Evidence From Eastern Arkansas, Jacob Askey

Biological and Agricultural Engineering Undergraduate Honors Theses

Unsustainable agricultural practices are putting a strain on freshwater supplies in many parts of the world. More efficient irrigation techniques are going to be critical to feed a growing population. Data from the 2016 Arkansas Irrigation Survey was used to assess the adoption of three Irrigation Water Management (IWM) practices: multiple inlet rice irrigation, tailwater recovery, and storage reservoirs. Results indicate that these practices do not always lead to reported pumping time reductions, a proxy for water use savings. Large variations in pumping time reduction are observed across producers for all three practices. A Tobit model was used to examine …

Characterizing Hydrothermal System Groundwater Dynamics Using Conservative And Non-Conservative Tracers: Insights And Implications Drawn From Study At The Taupo Volcanic Zone (New Zealand) And Valles Caldera (Usa), Joshua Michael Blackstock

Graduate Theses and Dissertations

Hydrothermal systems constitute an important component of heat and mass transport within Earth’s crust having direct and indirect impacts to society. Investigating contemporary hydrochemical differences and change are critical to evaluating and deconvolving factors that influence geothermal systems through time. In this dissertation, groundwater mixing approaches were used to study hydrochemical differences in two globally significant hydrothermal areas, which included fabrication of a low-cost dissolved CO2 monitoring for dissolved inorganic carbon (DIC). In the Taupo Volcanic Zone (New Zealand), empirical spatial relations were used to derive low-temperature groundwater end-members of Cl and Cl/Br for end-member mixing analysis (EMMA). Using EMMA, …

Phosphorus Runoff Risk Assessment In Karstic Regions Of The United States, Andrew N. Sharpley, Phillip D. Hays, Karl W. Vandevender, Mike B. Daniels

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

The Phosphorus (P) Index risk assessment tool has been widely adopted across the United States to identify and rank site vulnerability to P runoff as part of the Natural Resources Conservation Service nutrient management planning (NMP) process. However, limited success has been achieved in addressing the risk of P loss by subsurface flow pathways, despite its relative importance in certain areas of the United States, particularly in those U.S. states dominated by karst terrain. Here we review how states with varying land areas classified as having karst features address the risk of P runoff during the NMP process. Indices adopted …

An Evaluation Of Hybrid Traits, Yield, And Major Qtl Effect On Heterosis In Hybrid Soft Red Winter Wheat, Zachary James Winn

Graduate Theses and Dissertations

Wheat (Triticum aestivum L.) is a self-pollinating species that is most often bred as a recombinant inbred line. When two inbred individuals are crossed, they produce progeny which experience heterosis; heterosis is the increased robustness experienced due to the reintroduction of heterozygosity. Hybrid wheat may be produced through the use of chemical hybridization agents, yet the structure of wheat flowers decreases the efficiency of outcrossing. The objectives of this study were to: assess the amount of heterosis experienced by a population of hybrid wheat and observe the effect of major gene loci on yield and yield components, phenotype anthers extruded …

Rotational Tuning Of Transmembrane Helix Properties Based On The Precise Placements Of Aromatic And Charged Residues, Matthew J. Mckay

Graduate Theses and Dissertations

Designed model transmembrane peptides and oriented 2H and 15N solid-state nuclear magnetic resonance (NMR) spectroscopy were used to analyze how simple sequence modifications can influence peptide structure, behavior and dynamics as well as for determining the pKa of glutamic acid at the membrane interface. The GW5,19ALP23 (acetyl-GGALW(LA)6LWLAGA-amide) peptide framework adopts a well-defined tilted orientation in lipid bilayers (DLPC, DMPC and DOPC) and undergoes low amounts of dynamic motion. The sequence was initially modified by moving the Trp residues outwards to positions 4 and 20. This new sequence GW4,20ALP23 (acetyl-GGAW(AL)7AWAGA-amide) displays high amounts of signal averaging of NMR observables caused by …

Effects Of Dietary Copper, Zinc And Manganese Source And Level On The Acute Inflammatory Response Of Broilers, Chelsea Ellington

Graduate Theses and Dissertations

Trace minerals, particularly copper, zinc and manganese, play a role in ensuring optimal immune function. To examine the effects of diets containing different levels and sources of trace minerals on the inflammatory response, Cobb 500 broilers were fed four experimental diets containing 5 mg/kg Cu and 45 mg/kg of Zn and Mn or 10 mg/kg Cu and 90 mg/kg Zn and Mn in either the sulfate or hydroxychloride form. When the broilers were 5 weeks of age, 16 μg of lipopolysaccharide (LPS) was injected into the pulp of growing feathers (GF; 16 GF per chicken; 1 μg LPS per GF). …

Probing Of Carbohydrate-Protein Interactions Using Galactonoamidine Inhibitors, Jessica B. Pickens

Graduate Theses and Dissertations

Glycoside hydrolases are ubiquitous and one of the most catalytically proficient enzymes known, and thus understanding their mechanisms are crucial. Most research has focused on the interaction of the glycon of substrates and their inhibitors within the active site of glycoside hydrolases. The inhibitors employed to probe these interactions generally had small aglycons (i.e. a hydrogen atom, amidines, small aliphatic groups, or benzyl groups). Here, the interactions of the aglycon with glycoside hydrolases are examined by probing the active sites with a library of 25 galactonoamidines. The studies described in this dissertation aim to increase the understanding of stabilization of …

Development Of A Comsol Microdialysis Model, Towards Creation Of Microdialysis On A Chip With Improved Geometries And Recovery, Patrick Pysz

Graduate Theses and Dissertations

Microdialysis (µD) sampling is a diffusion-limited sampling method that has been widely used in different biomedical fields for greater than 35 years. Device calibration for in vivo studies is difficult for current non-steady state analytes of interest correlated with both inflammatory response and microbial signaling molecules (QS); which exist in low ng/mL to pg/mL with molecular weights over a wide range of 170 Da to 70 kDa. The primary performance metric, relative recovery (RR), relating the collected sample to the extracellular space concentration varies from 10% to 60% per analyte even under controlled bench-top conditions. Innovations in microdialysis device design …

Eastern Collared Lizard (Crotaphytus Collaris) Population Declines In Ozark Landscapes: An Assessment Of Environmental Constraints., Casey L. Brewster

Graduate Theses and Dissertations

The Eastern Collared Lizard (Crotaphytus collaris) has experienced extensive population declines over the past half century in the Ozark Mountains. Previous research suggests that glade habitat degradation through woody vegetation encroachment is an important factor resulting in population declines. In this dissertation I used information on time-energy budgets to investigate the link between habitat degradation and shifts in life history traits likely resulting in population declines of Eastern Collared Lizards (Crotaphytus collaris) in the Ozarks. In chapter one, I addressed the influence of dense woody vegetation encroachment on age-specific growth, body size, body condition and reproduction of C. collaris in …

Ecology And Conservation Of Shrubland Bird Communities In The Eastern Ghats Of Indi, Anant Deshwal

Graduate Theses and Dissertations

Anthropogenic disturbance, in its multiple facets represents a major threat to biodiversity and habitat quality. Consequently, extensive research is guided towards understanding anthropogenic disturbance and their effects on wildlife for development of wildlife management plans. However, for development of effective wildlife management plans it is imperative that we understand the habitat use and preference by local fauna along with effects of anthropogenic presence. In this dissertation, I studied the habitat usage and preferences of Shrubland birds in the Eastern Ghats of India during the pre-monsoon and post monsoon seasons. Eastern Ghats show a marked difference from pre-monsoon season to post-monsoon …

Turbidity And Ion Concentrations Vary With Land Use And Underlying Geology At The West Fork Of The White River, Erin E. Scott, Brian E. Haggard

Arkansas Water Resources Center Technical Reports

The West Fork of the White River (WFWR) watershed in northwest Arkansas is a trans-ecoregion watershed and is experiencing land-use changes, especially in the downstream portion of the watershed. The entire 54-km long river has been on the State’s 303(d) list of impaired waterbodies for turbidity, total dissolved solids (TDS), and sulfate for many years. The purpose of this study was to identify which part(s) of the river fail to meet applicable water quality standards (WQS) and to investigate possible sources of pollutants, whether human-caused or naturally occurring. Water samples were collected once or twice a month at 9 sites …

Cleavage Resistant Photoluminescent Proteins And Applications Thereof, Robert Beitle Jr., Rudra Palash Mukherjee, Thallapuranam Krishnaswamy Suresh Kumar, Joshua Sakon, David S. Mcnabb

Patents Granted

Photoluminescent proteins resistant to protein cleavage reagents are described herein. In being resistant to cleavage reagents, such photoluminesncent proteins can reduce the number of digestion or cleavage products, thereby simplifying quantification and purification of recombinant protein compositions. In some embodiments, a photoluminescent protein described herein comprises an amino acid sequence having an absence of internal methionine residues.

Arkansas Engineer, Fall 2019, University Of Arkansas, Fayetteville. College Of Engineering

Arkansas Engineer

No abstract provided.

Graduate Athletic Training Education Program Newsletter, Fall 2019, University Of Arkansas, Fayetteville. Athletic Training Education Program

Athletic Training Education Newsletter

No abstract provided.

Ansc, Fall 2019, University Of Arkansas, Fayetteville. Department Of Animal Sciences

Animal Science Newsletter

"Dedicated to the Alumni and Friends of the Animal Sciences Department, University of Arkansas."

Annual Report, 2018-2019, University Of Arkansas, Fayetteville. College Of Engineering

College of Engineering Annual Report

No abstract provided.

Arkansas Wheat Cultivar Performance Tests 2018-2019, R. E. Mason, R. G. Miller, D. E. Moon, J. P. Kelley

Arkansas Agricultural Experiment Station Research Series

Wheat cultivar performance tests are conducted each year in Ark- ansas by the University of Arkansas System Division of Agriculture’s Arkansas Agricultural Experiment Station, Department of Crop, Soil and Environmental Sciences. The tests provide information to companies developing cultivars and marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating cultivar recommendations for small-grain producers. The tests are conducted at the Northeast Research and Extension Center at Keiser, the Vegetable Substation near Kibler, the Lon Mann Cotton Research Station near Marianna, the Newport Extension Center near Newport, the Rohwer Research Station near Rohwer, the Pine Tree …

Engineered Fgf Compositions And Methods Of Use Thereof, Thallapuranam Krishnaswamy Suresh Kumar, David A. Zaharoff, Srinivas Jayanthi, Bhanuprasanth Koppolu, Rebecca Kerr, Kartik Balachandran, David S. Mcnabb

Patents Granted

The present invention relates to the development of stable mutants of FGF-1 and FGF-2. In particular, it relates to novel engineered FGF-1 and FGF-2 polypeptides as well as polynucleotides, DNA constructs, and vectors encoding such polypeptides. In another aspect, pharmaceutical compositions and hydrogels including the disclosed polypeptides, polynucleotides, DNA constructs, and vectors are provided. In a still further aspect, methods of treating conditions using the compositions disclosed herein are provided.