Digital Commons Network^™

Microbiota Analysis Of Chickens Raised Under Stressed Conditions, Rabindra Kumar Mandal, Tieshan Jiang, Robert Wideman Jr, Troy Lohrmann, Young Min Kwon

Poultry Science Faculty Publications and Presentations

A substantial progress has been made toward understanding stress-associated gut and extraintestinal microbiota. However, a comprehensive understanding of the extraintestinal microbiota of chickens raised under stressed conditions is lacking. In this study, chickens were raised on a wire-floor model to induce stress, and the microbiota in the gut (ceca) and extraintestinal sites (blood, femur, and tibia) were characterized at different ages (1, 17, and 56 days) using 16S rRNA gene microbiota profiling. Open reference OTU picking showed extraintestinal sites had a significantly higher number of unassigned OTUs compared to ceca across all ages of chickens. Extraintestinal sites of all ages, …

Endophyte-Infected Tall Fescue Affects Rumen Microbiota In Grazing Ewes At Gestation And Lactation, Jianmin Chai, Saleh Alrashedi, Ken Coffey, Joan Burke, Kristina M. Feye, Steven C. Ricke, Si Hong Park, J. Lannett Edwards, Jiangchao Zhao

Animal Science Faculty Publications and Presentations

Tall fescue (Schedonorus arundinaceus) is a cool-season perennial grass that is widely used as a forage for many livestock species including sheep. An endophyte (Neotyphodium coenophialum) in tall fescue produces ergot alkaloids that enhance plant survival but produce toxicosis in animals. The objective of this study was to investigate the rumen microbiota from gestation and lactation in ewes grazing tall fescue pastures with high (HA) or moderate (MA) levels of endophyte infection, and their relationship with serum parameters. Data were collected at the beginning of the study (d1), the week before initiation of lambing (d51), and at …

Methanosarcina Acetivorans Contains A Functional Isc System For Iron-Sulfur Cluster Biogenesis, Thomas M. Deere, Divya Prakash, Faith H. Lessner, Evert C. Duin, Daniel J. Lessner

Biological Sciences Faculty Publications and Presentations

Background

The production of methane by methanogens is dependent on numerous iron-sulfur (Fe-S) cluster proteins; yet, the machinery involved in Fe-S cluster biogenesis in methanogens remains largely unknown. Methanogen genomes encode uncharacterized homologs of the core components of the ISC (IscS and IscU) and SUF (SufBC) Fe-S cluster biogenesis systems found in bacteria and eukaryotes. Methanosarcina acetivorans contains three iscSU and two sufCB gene clusters. Here, we report genetic and biochemical characterization of M. acetivorans iscSU2.

Results

Purified IscS2 exhibited pyridoxal 5′- phosphate-dependent release of sulfur from L-cysteine. Incubation of purified IscU2 with IscS2, cysteine, and iron (Fe2+) resulted …

Minimal Structural Elements Required For Midline Repulsive Signaling And Regulation Of Drosophila Robo1, Haley E. Brown, Timonthy A. Evans

Biological Sciences Faculty Publications and Presentations

The Roundabout (Robo) family of axon guidance receptors has a conserved ectodomain arrangement of five immunoglobulin-like (Ig) domains plus three fibronectin type III (Fn) repeats. Based on the strong evolutionary conservation of this domain structure among Robo receptors, as well as in vitro structural and domain-domain interaction studies of Robo family members, this ectodomain arrangement is predicted to be important for Robo receptor signaling in response to Slit ligands. Here, we define the minimal ectodomain structure required for Slit binding and midline repulsive signaling in vivo by Drosophila Robo1. We find that the majority of the Robo1 ectodomain is dispensable …

Computer Science Teacher Survey, Josh B. Mcgee, Sarah C. Mckenzie

Arkansas Education Reports

In April/May of 2020, the University of Arkansas’ Office for Education Policy (OEP), in partnership with Arkansas Governor Asa Hutchinson's Computer Science and Cyber Security Task Force, fielded a survey with the 400+ Arkansas educators who at that time held a computer science endorsement (528), computer science approval code (5016), or computer science technical permit (5014) on their educator’s license. The survey received 153 responses, a nearly 40 percent response rate.

Inversion Modeling Of Japonica Rice Canopy Chlorophyll Content With Uav Hyperspectral Remote Sensing, Yingli Cao, Kailun Jiang, Jingxian Wu, Fenghua Yu, Wen Du, Tongyu Xu

Electrical Engineering Faculty Publications and Presentations

Chlorophyll content is an important indicator of the growth status of japonica rice. The objective of this paper is to develop an inversion model that can predict japonica rice chlorophyll content by using hyperspectral image of rice canopy collected with unmanned aerial vehicle (UAV). UAV-based hyperspectral remote sensing can provide timely and cost-effective monitoring of chlorophyll content over a large region. The study was based on hyperspectral data collected at the Shenyang Agricultural College Academician Japonica Rice Experimental Base in 2018 and 2019. In order to extract the salient information embedded in the high-dimensional hyperspectral data, we first perform dimension …

Pollen-Mediated Gene Flow And Transfer Of Resistance Alleles From Herbicide-Resistant Broadleaf Weeds, Amit J. Jhala, Jason K. Norsworthy, Zahoor A. Ganie, Lynn M. Sosnoskie, Hugh J. Beckie, Carol A. Mallory-Smith, Jun Liu, Wei Wei, Junming Wang, David E. Stoltenberg

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Pollen-mediated gene flow (PMGF) refers to the transfer of genetic information (alleles) from one plant to another compatible plant. With the evolution of herbicide-resistant (HR) weeds, PMGF plays an important role in the transfer of resistance alleles from HR to susceptible weeds; however, little attention is given to this topic. The objective of this work was to review reproductive biology, PMGF studies, and interspecific hybridization, as well as potential for herbicide resistance alleles to transfer in the economically important broadleaf weeds including common lambsquarters, giant ragweed, horseweed, kochia, Palmer amaranth, and waterhemp. The PMGF studies involving these species reveal that …

Berry Phenolic And Volatile Extracts Inhibit Pro-Inflammatory Cytokine Secretion In Lps-Stimulated Raw264.7 Cells Through Suppression Of Nf-Κb Signaling Pathway, Inah Gu, Cindi Brownmiller, Nathan Blaine Stebbins, Andy Mauromostakos, Luke Howard, Sun-Ok Lee

Food Science Faculty Publications and Presentations

Berries are a rich source of phytochemicals, especially phenolics well known for protective activity against many chronic diseases. Berries also contain a complex mixture of volatile compounds that are responsible for the unique aromas of berries. However, there is very limited information on the composition and potential health benefits of berry volatiles. In this study, we isolated phenolic and volatile fractions from six common berries and characterized them by HPLC/HPLC-MS and GC/GC-MS, respectively. Berry phenolic and volatile fractions were evaluated for an anti-inflammatory effect using lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells by measuring levels of pro-inflammatory cytokines and the nuclear factor-kappa …

Tuning Network Dynamics From Criticality To An Asynchronous State, Jingwen Li, Woodrow L. Shew

Physics Faculty Publications and Presentations

According to many experimental observations, neurons in cerebral cortex tend to operate in an asynchronous regime, firing independently of each other. In contrast, many other experimental observations reveal cortical population firing dynamics that are relatively coordinated and occasionally synchronous. These discrepant observations have naturally led to competing hypotheses. A commonly hypothesized explanation of asynchronous firing is that excitatory and inhibitory synaptic inputs are precisely correlated, nearly canceling each other, sometimes referred to as ‘balanced’ excitation and inhibition. On the other hand, the ‘criticality’ hypothesis posits an explanation of the more coordinated state that also requires a certain balance of excitatory …

Animal Science E-Newsletter, September 2020, University Of Arkansas, Fayetteville. Department Of Animal Sciences

Animal Science e-Newsletter

No abstract provided.

Summaries Of Arkansas Cotton Research 2019, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

Statewide, temperatures and precipitation were mostly above average (https://www.weather.gov/lzk/cli2019atxt.htm). The 2019 season was the wettest since 2015, and the 7th wettest on record. The wettest month was May, and the driest month was September. By average temperature, the coldest month was January, and the warmest month was August. Considering departures from normal, the most significant warmth occurred in September, which was 7.0 degrees above average. Many fields were muddy or underwater, given a lot of rain early in the year, and extensive river flooding (including the historic Arkansas River flood). It was far too wet in some areas …

Arkansas Wheat Cultivar Performance Tests 2019-2020, R. E. Mason, R. G. Miller, D. E. Moon, J. P. Kelley, J. Carlin

Arkansas Agricultural Experiment Station Research Series

Wheat cultivar performance tests are conducted each year in Ark- ansas by the University of Arkansas System Division of Agriculture’s Arkansas Agricultural Experiment Station, Department of Crop, Soil, and Environmental Sciences. The tests provide informa- tion to companies developing cultivars and marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating cultivar recommendations for small-grain producers. The tests are conducted at the Northeast Research and Extension Center at Keiser, the Vegetable Substation near Kibler, the Lon Mann Cotton Research Station near Marianna, the Newport Extension Center near Newport, the Rohwer Research Station near Rohwer, the Pine …

Sodium Butyrate Reduces Salmonella Enteritidis Infection Of Chicken Enterocytes And Expression Of Inflammatory Host Genes In Vitro, Anamika Gupta, Mohit Bansal, Basanta Raj Wagle, Xiaolun Sun, Narayan Rath, Annie Donoghue, Abhinav Upadhyay

Poultry Science Faculty Publications and Presentations

Salmonella Enteritidis (SE) is a facultative intracellular pathogen that colonizes the chicken gut leading to contamination of carcasses during processing. A reduction in intestinal colonization by SE could result in reduced carcass contamination thereby reducing the risk of illnesses in humans. Short chain fatty acids such as butyrate are microbial metabolites produced in the gut that exert various beneficial effects. However, its effect on SE colonization is not well known. The present study investigated the effect of sub-inhibitory concentrations (SICs) of sodium butyrate on the adhesion and invasion of SE in primary chicken enterocytes and chicken macrophages. In addition, the …

Defining Relictual Biodiversity: Conservation Units In Speckled Dace (Leuciscidae: Rhinichthys Osculus) Of The Greater Death Valley Ecosystem, Steven M. Mussmann, Marlis R. Douglas, David D. Oakey, Michael E. Douglas

Biological Sciences Faculty Publications and Presentations

The tips in the tree of life serve as foci for conservation and management, yet clear delimitations are masked by inherent variance at the species–population interface. Analyses using thousands of nuclear loci can potentially sort inconsistencies, yet standard categories applied to this parsing are themselves potentially conflicting and/or subjective [e.g., DPS (distinct population segments); DUs (Diagnosable Units-Canada); MUs (management units); SSP (subspecies); ESUs (Evolutionarily Significant Units); and UIEUs (uniquely identified evolutionary units)]. One potential solution for consistent categorization is to create a comparative framework by accumulating statistical results from independent studies and evaluating congruence among data sets. Our study illustrates …

Appetite Versus Hunger, Jamie Baum

Diet, Food, Exercise, and Nutrition (D-FEND)

Do you know the difference between appetite & hunger?

Learning Objectives:

Define & Understand the differences between appetite, hunger, & satiety

Understand energy balance and what contributes to energy balance

Evaluation Of A Novel Shallow Aggregate Ebb-And-Flood Culture System And Transplant Size Effects On Hydroponic Basil Yield, Samuel Doty, Ryan W. Dickson, Michael Evans

Horticulture Faculty Publications and Presentations

Ornamental bedding plant operations transitioning to leafy greens and herb production must decide whether to invest in new hydroponic equipment or modify existing culture systems for edible crops. In addition, common practices used to increase space-use and production efficiencies during bedding plant production may be modified for hydroponic leafy greens and herbs, such as purchasing large seedlings for transplant. The objective of the first experiment was to evaluate plant growth in a modified and novel shallow aggregate ebb-and-flood (SAEF) system intended for bedding plant growers with an emphasis on comparing yield across four basil (Ocimum basilicum) cultivars grown …

B.R. Wells Arkansas Rice Research Studies 2019, K.A. K. Moldenhauer, B. Scott, J. Hardke

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

In Ovo Administration Of Defined Lactic Acid Bacteria Previously Isolated From Adult Hens Induced Variations In The Cecae Microbiota Structure And Enterobacteriaceae Colonization On A Virulent Escherichia Coli Horizontal Infection Model In Broiler Chickens, Margarita A. Arreguin-Nava, Brittany D. Graham, Bishnu Adhikari, Melissa Agnello, Callie M. Selby, Xochitl Hernandez-Velasco, Christine N. Vuong, Bruno Solis-Cruz, Daniel Hernandez-Patlan, Juan David Latorre, Guillermo Tellez Jr., Billy M. Hargis, Guillermo Tellez-Isaias

Poultry Science Faculty Publications and Presentations

The effects of in ovo administration of a defined lactic acid microbiota (LAM), previously isolated from adult hens, in the cecae microbiota structure and Enterobacteriaceae colonization after exposure to virulent Escherichia coli during the hatching phase of broiler chickens were evaluated. Embryos inoculated with LAM showed a significant (P < 0.05) reduction of Enterobacteriaceae colonization at day-of-hatch (DOH) and day (d) 7. Furthermore, there was a significant increase in total lactic acid bacteria on DOH, body weight (BW) DOH, BW d7, and d0–d7 BW gain and reduced mortality d0–d7 was observed in the LAM group compared with that in phosphate-buffered saline (PBS) control. …

Animal Science E-Newsletter, August 2020, University Of Arkansas, Fayetteville. Department Of Animal Sciences

Animal Science e-Newsletter

No abstract provided.

Water Management Effects On Trace Gas Emissions Under Greenhouse Conditions From Direct-Seeded Hybrid Rice In A Silt-Loam Soil, Diego Della Lunga, Kristofor R. Brye, Trenton L. Roberts, Shelby G. Lebeau

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Water management regimes influence greenhouse gas emissions in rice (Oryza sativa L.) production. The objective of this study was to quantify methane (CH4 ), nitrous oxide (N2 O), and carbon dioxide (CO2 ) under direct-seeded conditions in the greenhouse to evaluate the effects of water regime on fluxes and growing-season-long emissions. Research was conducted during 2019 using a hybrid rice cultivar (RT 7311 CL) grown in a DeWitt silt loam (Albaqualf). Six plastic tubs, filled with 21 kg of soil and manually seeded, were arranged in a completely random design with two replications of three water regime treatments: i) flooded, …

Fate And Transport Of Phosphorus-Containing Land-Applied Swine Slurry In A Karst Watershed, A. N. Sharpley, M. B. Daniels, K. R. Brye, K. Vandevender, J. Burke, L G. Berry, P. Webb

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Phosphorus distribution in pasture soils underlain with karst geology was determined on a 0.10-ha grid in 2014, 2016, and 2018. Two fields (Fields 1 and 12) received swine slurry from a concentrated animal feeding operation, whereas another (Field 5) received mineral fertilizer. All fields were grazed by cattle and periodically hayed. Mean Mehlich-3 extractable P in the top 10 cm increased (p ≤ .05 level) for Fields 1 (59–91 mg kg⁻¹) and 12 (63–122 mg kg⁻¹) between 2014 and 2018, with little change for Field 5 (45–47 mg kg⁻¹). Over the 5-yr monitoring …

Soil Moisture, Temperature, And Oxidation-Reduction Potential Fluctuations Across A Furrow-Irrigated Rice Field On A Silt-Loam Soil, Diego Della Lunga, Kristofor R. Brye, Jordan M. Slayden, Christopher G. Henry, Lisa S. Wood

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Alternative water management practices for rice (Oryza sativa) production have been developed for water conservation purposes, such as the furrow-irrigated rice production system, which results in variable volumetric water content (VWC), temperature, and oxidation-reduction (redox) potential conditions. The spatial and temporal variations in environmental factors can affect greenhouse gas (GHG) emissions. The objective of this field study was to document and evaluate the continuous variations in soil water content, temperature, and redox potential over two growing seasons (2018 and 2019) among site positions (up-, mid-, and down-slope) and tillage treatments [conventional tillage (CT) and no-tillage (NT)] under furrow-irrigated …

Testing Flowering Perennial Plants In A Bermudagrass (Cynodon Spp.) Lawn, Paige Elizabeth Boyle, Michelle Wisdom

Horticulture Faculty Publications and Presentations

Most pollinating insects require a season-long succession of floral resources to fulfill life-cycle requirements. Incorporating forbs into turfgrass sites may create a season-long sequence of flowers to support foraging pollinators. However, persistence of forbs in warm-season turfgrasses such as bermudagrass (Cynodon spp.) may be affected by the competitive nature of the turfgrass and routine management practices such as mowing. A 2-year study was conducted to evaluate seven forbs (Bellis perennis L., Lotus corniculatus L., Prunella vulgaris L., Trifolium fragiferum L. ‘Fresa’, Trifolium repens L. ‘Durana’ and ‘Resolute’, Trifolium subterraneum L.) for persistence and ability to produce floral resources …

Design And Testing Of A Multi-Depth Soil Temperature Sensor Array Intended As A Candidate Irrigation Scheduling Tool, Lillian Glaeser

Biological and Agricultural Engineering Undergraduate Honors Theses

A crucial measure in the advancement of water conservation and sustainable agricultural strategies is increasing efficiencies of irrigation systems. Because of the lack of availability of affordable, durable, and scalable soil moisture monitoring devices, this thesis proposes a low-cost, multi-depth soil temperature sensor array as a candidate for monitoring soil moisture content and guiding farmer’s irrigation scheduling. Propagation of diurnal temperature waves through soil is dependent on the thermal diffusivity of the soil, which can be determined by examining temperature waves at different soil depths. Thermal diffusivity is dependent on several factors, though, in undisturbed soil, water has the largest …

Chemistry And Biochemistry Authored Faculty Publications: Snapshot Of The Ranking Of Journals In Which They Published Based On Data From Journal Citation Reports (Jcr), Lutishoor Salisbury, Yang Tian, Jeremy Smith

University Libraries Faculty Publications and Presentations

This report presents a snapshot of the research output of the Chemistry and Biochemistry Department at the University of Arkansas for the period 2015-2019. The contents of the report were generated using Web of Science Core Collection databases and the Journal Citation Report only. It highlights the journals in which the researchers published and the ranking of those journals.

Using University Research In Food Science To Study Their Characteristics And To Model The Research And Information Needs Of Researchers, Lutishoor Salisbury, Jeremy Smith, Abayomi Omotola Omolewu

University Libraries Faculty Publications and Presentations

Using University Research in Food Science to Study their Characteristics and to Model the Research and Information Needs of Researchers.

Making collection management decisions of resources is important and challenging. The challenges arise from finding the balance between the mission of libraries to meet the needs of a wide range of users; the high cost of subscription to journals and databases; the escalating annual increases imposed by publishers, and limited budget for collection management activities.

By identifying and analyzing the research output from faculty and graduate students from the Food Science Department to determine where they published and the sources …

Dissecting The Cellular Control Of Septin Organization In A Global Cereal Killer, Nawaraj Dulal

Graduate Theses and Dissertations

Rice blast disease, caused by the filamentous fungus Magnaporthe oryzae, destroys sufficient rice each year to feed 60 million people, and is a serious threat to global food security. A wheat-adapted lineage of M. oryzae now poses threat to global wheat production. Rice blast disease is currently controlled using limited fungicides, and the emergence of fungicide resistance within M. oryzae populations is a growing concern. There is a pressing need to identify new classes of fungicides to control the disease, which requires better understanding of the basic biology of the pathogen. To establish disease, M. oryzae forms a specialized dome …

The Role Of Designers In Promoting Healthy Masculinity With An Approach To Stopping Violence Against Women And Girls, Fatemeh Abolbashari

Graduate Theses and Dissertations

While awareness about violence against women and girls is growing, there is still a lack of evidence about what changes behavior to prevent it from happening. As a graphic designer, I wanted to find the root problem of this issue and work towards a solution. Through my research and questioning, I concluded that this problem begins with men’s thoughts on hierarchy, language, and behavior, and supported by a prevalence of toxic masculinity in men’s culture.

Where does this violence come from? What has society done to raise this kind of man? Violence against women and girls is being couched in …

Taxonomic And Genetic Diversity, And Pathogenicity Of Diaporthe Species Associated With Soybean, Fakhir Eraheem Hameed Al Shuwaili

Graduate Theses and Dissertations

Diaporthe species (anamorph: Phomopsis) are associated with a wide range of plant hosts as plant pathogens, asymptomatic endophytes, and saprobes. One of these hosts is soybean, which is one of the most important crops in U.S. agriculture. Several Diaporthe species cause important diseases on soybean in the U.S., and specifically in Arkansas. The taxonomy, genetic diversity, and pathogenicity of Diaporthe species associated with asymptomatic infection of soybean are rarely studied with accurate molecular tools. Therefore, this dissertation aimed to assess the diversity and boundaries of Diaporthe associated with soybean in Arkansas. Furthermore, pathogenicity and alternative lifestyles were assessed among Diaporthe …

Assessing Differential Item Functioning In The Perceived Stress Scale, Nana Amma Berko Asamoah

Graduate Theses and Dissertations

When an item on a test functions differently for subgroups of respondents with respect to an exogenous variable (or covariate) after conditioning on the latent variable of interest, the item is said to exhibit Differential Item Functioning (DIF). The 10-item Perceived Stress Scale (PSS10) is administered to respondents via MTurk to quantify “perceived stress” and identify if items on the scale function differently for specific subgroups defined by age, sex, race, marital status, number of children, employment status and social media usage.

The purpose of this study was to compare traditional DIF detection approaches (Mantel-Haenszel, logistic regression, likelihood ratio test …