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Cross-Facility Reliable Deep Learning Based Beef Marbling Assessment Via Unsupervised Domain Adaptation Regression, Samuel Vinson
Cross-Facility Reliable Deep Learning Based Beef Marbling Assessment Via Unsupervised Domain Adaptation Regression, Samuel Vinson
Biological and Agricultural Engineering Undergraduate Honors Theses
Inconsistent quality grading in beef production leads to inefficiencies, economic disparity, and consumer mistrust. While USDA meat grading traditionally relies on skilled visual inspectors, these human evaluations suffer from cross-facility variability and subjectivity. This paper introduces the first known application of unsupervised domain adaptation regression for cross-facility beef marbling score prediction—an innovation that improves generalization across diverse environments in the beef supply chain. Utilizing numerical scores ranging from 100-900, the research employed convolutional neural networks (CNNs), including ResNet, VGG, and AlexNet architectures. The study specifically introduced and validated a unified unsupervised domain adaptation regression method using the ResNet-50 architecture to …
Retrospective Analysis Of Implemented Project Management Within A Small-Scale Student Engineering Project: Beng Fountain Wars Team Case Study, Holli E. Jenkins
Retrospective Analysis Of Implemented Project Management Within A Small-Scale Student Engineering Project: Beng Fountain Wars Team Case Study, Holli E. Jenkins
Biological and Agricultural Engineering Undergraduate Honors Theses
This honors thesis provides a retrospective analysis of how the 2025 inaugural University of Arkansas’s ASABE Fountain Wars Student Team could be improved through project management. Project commitment, early stakeholder influence, rich communication, and a detailed project plan are all attributes of successful projects, and the team’s strengths and weaknesses within these categories were determined through a team poll. After the poll was conducted, three methodologies, Agile, Hybrid, and Waterfall, were assessed to determine how they would effectively address the team’s weaknesses. A weighted objectives table was created to compare each methodology’s effectiveness across the management categories. The analysis revealed …
Developing Transparent Sustainability Data Systems In A Poultry And Pet Food Company: An Internship Case Study, Catherine L. Warren
Developing Transparent Sustainability Data Systems In A Poultry And Pet Food Company: An Internship Case Study, Catherine L. Warren
Biological and Agricultural Engineering Undergraduate Honors Theses
My honors thesis examined my internship experiences and its implications on sustainability data gathering and reporting in poultry and pet food companies. It highlighted my internship projects, the skills I developed, and the connections to sustainability data transparency and reliability in a corporate environment.
In my thesis, I shared details about my internship. I described what projects I worked on, the places I visited, the hands-on experience I gained, and the different teams I worked with throughout the internship duration. I discussed the analytic and professional skills I developed through conducting a waste audit, developing Tableau dashboards, and helping to …
Monitoring Streambank Morphology Using Iphone Lidar Technology, Lily C. Stitt
Monitoring Streambank Morphology Using Iphone Lidar Technology, Lily C. Stitt
Biological and Agricultural Engineering Undergraduate Honors Theses
Monitoring stream morphology is crucial for maintaining the health and safety of fluvial environments, particularly as these naturally dynamic systems are shifted out of equilibrium due to climate and land-use change. Quantifying changes in stream geometry are useful for infrastructure design, water quality assessment, water resource management, and stream restoration, among other applications. Traditional stream surveying methods contain numerous cost limitations that inhibit affordable and accessible stream surveying needed for evaluating stream geometry with high accuracy and larger spatial scales (>100s of ). In recent years, several advancements have been made in the use of Light Detection and Ranging …
Per- And Polyfluoroalkyl Substances (Pfas) In Cave And Withrow Springs, Arkansas, Luke A. Ahlemeier
Per- And Polyfluoroalkyl Substances (Pfas) In Cave And Withrow Springs, Arkansas, Luke A. Ahlemeier
Biological and Agricultural Engineering Undergraduate Honors Theses
The class of chemicals known as Per- and Polyfluoroalkyl Substances (PFAS) are believed to be widespread in the natural environment, but the full extent of PFAS prevalence is not well understood, particularly in karst springs. The objectives of this study were to (1) collect water samples from Cave Springs and Withrow Springs, Northwest Arkansas, (2) measure for concentrations of 40 PFAS compounds using EPA Method 1633, and (3) compare differences in PFAS concentrations and discuss potential sources of PFAS for each spring. Each spring was sampled on September 29, 2025, October 13, 2025, and November 10, 2025, and water samples …
Dried Water Treatment Residuals Might Be An Option To Reduce Soluble Reactive Phosphorus Release From Soils, Katie B. Mckay
Dried Water Treatment Residuals Might Be An Option To Reduce Soluble Reactive Phosphorus Release From Soils, Katie B. Mckay
Biological and Agricultural Engineering Undergraduate Honors Theses
Nonpoint source pollution from agricultural landscapes contributes to freshwater eutrophication, with legacy phosphorus (P) in soils potentially driving soluble reactive phosphorus (SRP) losses to surface waters. The objectives of this study were to evaluate SRP adsorption by dried water treatment residuals (WTRs) produced by Beaver Water District, quantify reductions in soil SRP release across increasing WTR application rates, and compare dried WTR performance to prior WTR studies.
Dried WTRs (~98% solids) were assessed using batch sorption experiments and soil core incubations. Phosphorus sorption was measured by mixing WTRs with CaCl2 solutions containing increasing SRP concentrations and fitting results to …
Enhancing Food Security In Stann Creek, Belize Using Meat Rabbits: Engineering Perspective, Lindsey Garretson
Enhancing Food Security In Stann Creek, Belize Using Meat Rabbits: Engineering Perspective, Lindsey Garretson
Biological and Agricultural Engineering Undergraduate Honors Theses
The purpose of this paper is to discuss the engineering perspective within the overall project of using meat rabbits to enhance food security and nutrition within Stann Creek, Belize. From the engineering perspective, the overarching goals were like the overarching project goals including the construction and materials required guide within the manual, designing potential upgrades to the design including a solar and battery system and an automatic watering system, and to design the system to be “practically sustainable” where possible. The constraints for this project included cost, the size of land dedicated for the rabbitry, and the operational and environmental …
A Systems Approach To Nitrogen Fertilizer Management: Modeling Emissions And Runoff Pathways In Arkansas Agriculture, Avonelle Lindon
A Systems Approach To Nitrogen Fertilizer Management: Modeling Emissions And Runoff Pathways In Arkansas Agriculture, Avonelle Lindon
Biological and Agricultural Engineering Undergraduate Honors Theses
Nitrogen fertilizer management is a critical challenge in modern agriculture. This thesis develops a system dynamics model of nitrogen in Arkansas rice and corn systems to explore barriers to translating agricultural research into practical farmer guidance. The modeling process revealed key challenges: (1) scarcity of Arkansas-specific factors and best management practice (BMP) effectiveness data; (2) tension between model comprehensiveness and accessibility; (3) misalignments in conservation incentive structures; and (4) difficulty in validating models without field measurements. The resulting model demonstrates basic nitrogen cycle processes and BMP integration, but validation against published literature revealed significant issues with predictive accuracy. These findings …
Uav-Based Sensor Integration For In-Situ Lake Water Quality Monitoring, Alec R. Campbell
Uav-Based Sensor Integration For In-Situ Lake Water Quality Monitoring, Alec R. Campbell
Biological and Agricultural Engineering Undergraduate Honors Theses
Surface water monitoring is often constrained by limited spatial and temporal coverage due to the labor-intensive nature of traditional sampling methods, particularly in environments that are difficult to access or pose safety risks. Unmanned aerial vehicles (UAVs) offer a promising solution by enabling more frequent, spatially distributed, and cost-effective data collection. This study presented the design, development, and field evaluation of a UAV-based system for real-time, in-situ water quality monitoring. The system integrated multiple sensors, including oxidation-reduction potential (ORP), RGB spectrometry, pH, electrical conductivity (EC), dissolved oxygen (DO), and a multispectral spectrometer within a UAV platform.
Field testing was conducted …
Design And Performance Overview Of The Inaugural University Of Arkansas Beng Fountain Wars Team, Autumn Jones
Design And Performance Overview Of The Inaugural University Of Arkansas Beng Fountain Wars Team, Autumn Jones
Biological and Agricultural Engineering Undergraduate Honors Theses
This honors thesis describes the engineering design process for the first ASABE Fountain Wars submission by a student team at the University of Arkansas in over a decade. Fountain Wars has a technical and aesthetic task, and the focus of this thesis is the design of the technical task. In 2025, this task was the “shoot out.” The objective was to get objects from a small pool into two 5-gallon buckets 10-15 feet away. The power for initiating the launch had to be provided by the pump. The objects were required to start at the surface of the water.
The …
Construction Impacts On Nutrient Loads In An Urban Stream In The Ozark Highlands, Matthew B. Wilson
Construction Impacts On Nutrient Loads In An Urban Stream In The Ozark Highlands, Matthew B. Wilson
Crop, Soil and Environmental Sciences Undergraduate Honors Theses
Degradation of water quality is primarily caused by human activity, including urbanization. Urban stream syndrome can be characterized as a stream that has undergone physical, chemical, and biological changes as a result of urbanization, which can include an influx of nutrients that can drive unwanted plant and algae growth. It is known that construction can result in untreated nutrients entering a stream; however, it is not understood how construction affects downstream nutrient transport. The objective of this study was to understand the impacts of construction on urban water quality in Tanglewood Branch in Fayetteville, Arkansas, in the Ozark Highlands, which …
Characterization Of Snrk1 Signaling In Rice Growth, Reproduction, And Stress Responses Using Phenotypic And Omics Analysis, Maria Clara Faria-Bates
Characterization Of Snrk1 Signaling In Rice Growth, Reproduction, And Stress Responses Using Phenotypic And Omics Analysis, Maria Clara Faria-Bates
Graduate Theses and Dissertations
SnRK1 is an evolutionarily conserved protein kinase belonging to the SNF1/AMPK family that is composed of a catalytic subunit (α) and two regulatory subunits (β and βγ), and it has a major role in adjusting growth in response to the energy status. SnRK1 phosphorylates and alters the activities of enzymes involved in metabolism and regulates gene expression by altering the activity of chromatin-remodeling enzymes or transcription factors. Rice contains three functional paralogs of SnRK1α: OsSnRK1αA (Os05g0530500), OsSnRK1αB (Os8g0484600), and OsSnRK1αC (Os03g0289100). Although SnRK1 is known to play a central role in plant growth and stress responses, the functional specialization of …
Conservation Agriculture Effects On Greenhouse Gas Emissions From Cotton, Soybean, And Corn In Southeast Arkansas, Jonathan Benjamin Brye
Conservation Agriculture Effects On Greenhouse Gas Emissions From Cotton, Soybean, And Corn In Southeast Arkansas, Jonathan Benjamin Brye
Graduate Theses and Dissertations
The slow degradation of natural resources has resulted in the focus of agriculture shifting towards conservation practices, such as reduced/no-tillage, cover crops (CC), and organic amendments (i.e., biochar), that conserve and improve natural resource quality. The objectives of this thesis were to i) quantify the impacts of biochar rate (i.e., 0, 2000, and 4000 kg ha-1) and ii) the combination of CC and tillage practice on carbon dioxide (CO¬2), methane (CH4), and nitrous oxide (N2O) fluxes, growing-season-long emissions, global warming potential (GWP), soil properties, and plant response from a minimally tilled cotton (Gossypium hirsutum)-corn (Zea mays) rotation and a soybean …
The Influence Of Additional Zinc Supplementation During Gestation On The Trace Mineral Concentrations Of Tissues, Milk, And Calf Serum At Birth, Hunter Miller
Animal Science Undergraduate Honors Theses
Minerals are known to play a key role in the immune system, growth, and development of animals. An important part of a calf's life is receiving colostrum immediately after birth, which provides the calf with immunoglobulins and necessary nutrients that are required. Furthermore, the gestation period is another very important aspect of the calf's life where they can take in the necessary nutrition. This study compared how two different supplements fed during the gestation period ultimately affected the mineral concentrations of the colostrum, placenta, calf plasma, and cow liver. The supplements that were provided were a 1) control that provided …
The Effect Of Blue-Green Light On Behavioral Repertoire And Fear Responses In Broiler Chickens, Addie Walker
The Effect Of Blue-Green Light On Behavioral Repertoire And Fear Responses In Broiler Chickens, Addie Walker
Animal Science Undergraduate Honors Theses
The broiler chicken industry has increased the number of birds produced each year,
making the mitigation of stressors that affect broiler health and meat quality increasingly
important. New management strategies, like lighting protocols, could help combat stressors and
promote behavioral indicators of positive welfare. This study compared the effects of blue-green
and white light on broiler fear response using a tonic immobility test and behavioral repertoire in
the home pen using continuous focal sampling. Day-of-hatch male broiler chicks (N=600) were
randomly divided into 12 pens (n=50 chicks/pen). The pens had either a white LED light
(control, n=6) or a blue-green …
Developing A Questionnaire For The Interoceptive Awareness Of Stuttering, Leslie Williams
Developing A Questionnaire For The Interoceptive Awareness Of Stuttering, Leslie Williams
Graduate Theses and Dissertations
Stuttering is commonly measured through its overt characteristics (blocks, prolongations, and repetitions). This equates to stuttering “severity,” or the frequency of these behaviors. This method, however, does not reflect the lived experience of adults who stutter. This “measurement problem” has been the subject of numerous studies. In this graduate thesis, the measurement problem is addressed by using qualitative data previously gathered by Malcolm (2024) from adults who stutter about the internal experience of stuttering to create survey. Quotes from adults who stutter were gathered, categorized, edited for clarity, and formatted into a survey, The Multidimensional Interoceptive Awareness of Stuttering Scale …
Exploring The Spectrum Of Anxiety During Concussion Recovery: A Comprehensive Examination Of Symptom-Based Anxiety Disorders, Kori J. Durfee
Exploring The Spectrum Of Anxiety During Concussion Recovery: A Comprehensive Examination Of Symptom-Based Anxiety Disorders, Kori J. Durfee
Graduate Theses and Dissertations
Background: Anxiety is common following a concussion, arising from both psychosocial and physiological changes, and can manifest in diverse ways. Despite its complexity and association with worse outcomes, post-concussion anxiety is often assessed as a single construct. Characterizing anxiety presentations following injury may improve the identification of clinically meaningful symptoms and help inform future targeted interventions that can improve concussion outcomes. Purpose: The primary purpose was to determine if symptom-based anxiety disorders occur in adults with concussion and identify pre- and post-injury predictors associated with these disorders. Additionally, the clinical utility and predictive abilities of symptom-based anxiety disorders and variability …
Arkansas Soybean Research Studies 2024, Jeremy Ross
Arkansas Soybean Research Studies 2024, Jeremy Ross
Arkansas Agricultural Experiment Station Research Series
The 2024 Arkansas Soybean Research Studies includes research reports on topics pertaining to soybean across several disciplines from breeding to post-harvest processing. Research reports contained in this publication may represent preliminary or only data from a single year or limited results; therefore, these results should not be used as a basis for long-term recommendations. Several research reports in this publication will appear in other University of Arkansas Division of Agriculture’s Arkansas Agricultural Experiment Station publications. This duplication is the result of the overlap in research coverage between disciplines and our effort to inform Arkansas soybean producers of the research being …
Overwhelmed By Competing Priorities: Exploring Women’S Motivational Conflict Across The Menstrual Cycle, Arial S. Bloshinsky
Overwhelmed By Competing Priorities: Exploring Women’S Motivational Conflict Across The Menstrual Cycle, Arial S. Bloshinsky
Graduate Theses and Dissertations
This study examined whether the biological context of the menstrual cycle predicted overwhelming feelings due to competing bonding and achievement motivations (i.e., motivational conflict). Building on the motivational shift hypothesis, it was predicted that the luteal phase, characterized by elevated progesterone and heightened bonding motivation, would be associated with more overwhelming feelings from motivational conflict. To assess potential changes across the menstrual cycle, a within-person design was used where women reported their overwhelming feelings daily. Primary analyses did not support the hypothesis. Overwhelming feelings from motivational conflict did not differ across cycle phases. However, the expected mid-cycle peak in sexual …
Impacts Of Climate-Smart Agriculture On Greenhouse Gas Emissions In Southeast Arkansas Cotton, Cassandra Seuferling
Impacts Of Climate-Smart Agriculture On Greenhouse Gas Emissions In Southeast Arkansas Cotton, Cassandra Seuferling
Graduate Theses and Dissertations
Increasing concerns of climate change have led to the evaluation of greenhouse gas (GHG) emissions [i.e., carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O)] from agroecosystems, particularly cotton (Gossypium hirsutum) production. Technology improvement and conservation agriculture methods [i.e., reduced-tillage (RT) instead of conventional tillage (CT) and the use of cover crops (CC)] require further evaluation regarding GHG emissions from Arkansas cotton production. The first objective of the study was to evaluate four gas-flux-determination methods [i.e., linear or exponential regression models, with negative fluxes (WNF) included in the dataset or replacing negative fluxes (RNF)] on CO2, CH4, and N2O fluxes …
Marvelous And Disturbing: Surreal Imagery, Elaboration And The Limits Of Persuasion In Women's Health Journalism, Shelby Elizabeth Gill
Marvelous And Disturbing: Surreal Imagery, Elaboration And The Limits Of Persuasion In Women's Health Journalism, Shelby Elizabeth Gill
Graduate Theses and Dissertations
Since the 1930s, surrealism has lent its abstract hand to journalism, offering a visual language for topics that resist literal depiction. Given its historical and often fraught association with the female form, surrealist imagery has found a natural home in editorial coverage of women's health, most recently in reporting on endometriosis, a condition whose invisible, stigmatized and difficult-to-articulate experience makes conventional photojournalism an inadequate representational tool. The present study experimentally compared surreal imagery, realistic imagery and a text-only control condition to assess their effects on knowledge acquisition, comprehension, attitudinal responses, perceived risk and behavioral intentions related to endometriosis in a …
Sensing Rna’S Conformation With A Solid-State Nanopore Device At Various Ph And Temperature, Haopeng Li
Sensing Rna’S Conformation With A Solid-State Nanopore Device At Various Ph And Temperature, Haopeng Li
Graduate Theses and Dissertations
This study uses solid-state nanopore technology to investigate the stability and dynamics of RNA molecules under thermal stress and pH fluctuations. Two distinct mRNA samples were analyzed: (1) heat shock protein (HSP)-encoding mRNA extracted from Sulfolobus solfataricus P2, a thermophilic archaeon inhabiting extreme environments such as geothermal sulfuric hot springs (pH < 4, temperature range: 65–90°C), and (2) total RNA extracted from White Leghorn chickens, which thrive under standard physiological conditions. ΦX174 virion single-stranded DNA (ssDNA) were included as a comparative reference. Nanopores, fabricated in silicon nitride membranes, were used to electrically characterize the structural properties of individual RNA molecules in solution, close to their native environments. The nanopore membrane separates two chambers containing salt buffer solution, where ionic current is the primary measurement parameter. When a voltage bias is applied across the membrane, ion flow and an ionic current can be measured. Charged RNA molecules driven translocation through the nanopore induces pore resistance changes, resulting in detectable current blockade events. Variations in these events provide insights into RNA secondary structure and conformational dynamics. By systematically altering pH and temperature, this study elucidates the effects of environmental stressors on RNA stability and structural integrity. The findings reveal distinct responses between thermophilic HSP-encoding mRNA and mesophilic RNA, underscoring the adaptive mechanisms of thermophilic RNA under extreme conditions. These results contribute to a better understanding of RNA behavior under stress and establish a foundation for future applications in RNA-based diagnostics and biotechnology development.
Fret Studies On The Binding Of Fibroblast Growth Factor (Fgf) Proteins To The Hard Corona Of Cuins2-Based Quantum Dots, Colette Robinson
Fret Studies On The Binding Of Fibroblast Growth Factor (Fgf) Proteins To The Hard Corona Of Cuins2-Based Quantum Dots, Colette Robinson
Graduate Theses and Dissertations
Fibroblast Growth Factor (FGF) has been shown to be an important protein in angiogenesis, which is critical in developmental biology and wound healing but has also been implicated in cancer metastasis. FGF interacts with an FGF Receptor (FGFR) to signal the onset of angiogenesis but the detailed mechanism of angiogenesis, and particularly its regulation, is still largely unknown. Imaging the interaction of FGF with FGFR in biologically-relevant environments, such as directly in cells, is key towards furthering this understanding and may eventually aid in the development of better cancer treatments. CuInS2/ZnS quantum dots (QDs) are an attractive fluorescent probe to …
Nadh Flim Reveals Cellular Bioenergetics And Mitochondrial Morphology During Drosophila Convergent Extension, Maria Espana Pena
Nadh Flim Reveals Cellular Bioenergetics And Mitochondrial Morphology During Drosophila Convergent Extension, Maria Espana Pena
Graduate Theses and Dissertations
Mitochondria are dynamic organelles that can fragment or fuse to support different bioenergetic demands and cellular processes, although the role of mitochondrial fission and fusion during embryonic development is not well understood. Fluorescence lifetime imaging (FLIM) of the mitochondrial cofactor NADH can be used to visualize mitochondrial networks and infer aspects of cellular bioenergetics in a label-free manner. We used NADH FLIM to test whether germband cells undergo changes in cellular metabolism during Drosophila convergent extension (CE)––a process in which hundreds of epithelial cells undergo coordinated intercalation to elongate the embryo. Contrary to our expectations, we did not observe significant …
Mind The Gap: Exploring Sociocultural And Educational Factors Contributing To The Gender-Based Achievement Gap In Early Elementary Ela, William Matthew Mcgee
Mind The Gap: Exploring Sociocultural And Educational Factors Contributing To The Gender-Based Achievement Gap In Early Elementary Ela, William Matthew Mcgee
Graduate Theses and Dissertations
This study examined the sociocultural and educational factors contributing to the gender-based achievement gap in early elementary English Language Arts (ELA). While research has examined the gender-based achievement gap related to female-identifying students in STEM disciplines, there is less emphasis on the underperformance of male-identifying students in ELA. Earlier research often emphasized biological explanations rather than the influence of environment and instructional practices. This study was guided by the Expectancy-Value Theory and Social Role Theory to better understand how home environments, gender norms, instructional practices, and student perception of literacy influence student engagement and performance in Kindergarten through third grade. …
From Phenology To Yield Zones: Functional Clustering Of Satellite Time Series For Crop Growth Monitoring, Ikram Morso
From Phenology To Yield Zones: Functional Clustering Of Satellite Time Series For Crop Growth Monitoring, Ikram Morso
Graduate Theses and Dissertations
Remote sensing and land surface phenology provide a strong basis for characterizing crop development, but their usefulness for within-field yield-zone delineation depends on whether crop development is analyzed as a phenological trajectory and whether the resulting zones are evaluated with spatially defensible statistics. These questions are especially relevant in soybean production because soybean is one of the most important row crops in the United States and the country’s dominant oilseed crop. This relevance is strong in Arkansas, where soybean is a major component of agricultural production. In soybean production, within-field yield variability reflects the combined influence of soil properties, stand …
Lumbopelvic Kinematics And Muscular Co-Contractions In Walking Prior To And Immediately Following A Bout Of Prolonged Standing., Stephanie Cotariu
Lumbopelvic Kinematics And Muscular Co-Contractions In Walking Prior To And Immediately Following A Bout Of Prolonged Standing., Stephanie Cotariu
Graduate Theses and Dissertations
Background: Prolonged standing is a common daily activity in a wide array of occupations, and approximately 44% of sample populations develop low back pain (LBP) during prolonged standing. Prolonged standing-induced LBP developers (“Pain Developers”) are three times more likely to develop clinical LBP and can therefore be considered a pre-clinical LBP population. Changes in typical gait have been observed in LBP patients, and walking has been hypothesized as being protective or preventive against LBP development. However, it is unknown whether Pain Developers exhibit similar kinematic adaptations to chronic LBP patients during common daily activities, such as walking. The exact muscular …
Effects Of Wetting And Drying On Nutrient Mobilization In Aquatic Systems, Karessa Grace De La Paz
Effects Of Wetting And Drying On Nutrient Mobilization In Aquatic Systems, Karessa Grace De La Paz
Graduate Theses and Dissertations
Wetting and drying cycles are a fundamental, but underexplored, control on nutrient mobilization and transport in aquatic ecosystems. Such intermittency impacts approximately 60% of the world's streams and rivers, and the extent is expected to increase with future climate change. Additionally, aging reservoir infrastructure is driving restoration efforts, like large-scale drawdowns, that can expose benthic sediments to extended dry periods before refilling. Despite the prevalence of wet-dry cycles, how they influence nutrient transport and mobilization across different aquatic ecosystems remains poorly understood. To address this gap, this thesis presents two complementary studies exploring nutrient dynamics in Arkansas aquatic ecosystems that …
Fast Hyperspectral And Super-Resolved Mapping Of Lipid Membrane Polarity With Single-Molecule Sensitivity, Elric Dion Pott, Meek Yang, James Ethan Batey, Jole Embree, Bin Dong
Fast Hyperspectral And Super-Resolved Mapping Of Lipid Membrane Polarity With Single-Molecule Sensitivity, Elric Dion Pott, Meek Yang, James Ethan Batey, Jole Embree, Bin Dong
Chemistry & Biochemistry Faculty Publications and Presentations
Cell membranes display nanoscale heterogeneity in lipid composition and organization that regulates vital biological processes yet remain challenging to resolve with conventional imaging. We introduce spectral phasor single-molecule localization microscopy (SP-SMLM), a hyperspectral and super-resolution method that combines wavefront-like optical filtering with single-molecule imaging for simultaneous spatial and spectral analysis. A lab-built three-channel imager with sine/cosine filters encodes emission spectra of single molecules into the phasor space, enabling high-throughput, high-SNR mapping of membrane polarity at sub-50 nm spatial and 15 s temporal resolutions. Through simulation, we validate that the phasor angle correlates with the spectral mean for single dye molecules. …
Prompt Engineering For Lawyers: We Will Add Your Distinctiveness To Our Own: Iterative Refinement And Prompt Chaining, Cliff Mckinney
Prompt Engineering For Lawyers: We Will Add Your Distinctiveness To Our Own: Iterative Refinement And Prompt Chaining, Cliff Mckinney
Arkansas Law Notes
In the last edition, we explored two advanced prompt engineering techniques, chunking and few-shot prompting. In this edition, we will examine two more useful techniques: iterative prompt refinement and prompt chaining. Iterative prompt refinement involves repeatedly rephrasing and refining questions to lead to a better response. Prompt chaining involves guiding the artificial intelligence through related multi-step reasoning.
At first glance, iterative prompt refinement and prompt chaining may seem like the same concept, but the key difference lies in their approach. Refinement improves a single prompt through repeated adjustments, whereas chaining links a series of prompts together to tackle different stages …