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Thermal Inactivation Kinetics Of Salmonella In Milk Powder As Impacted By Water Activity And Powder Type, Erika Litta Kadas

Graduate Theses and Dissertations

Historically, low-water activity (aw) foods (aw <0.65) were considered to be microbiologically safe for consumption. However, these foods have been implicated in outbreaks of pathogens such as Salmonella enterica, at a frequency that surely challenges this assumption of safety. Although usually implicated in outbreaks involving poultry and egg products, Salmonella spp. have frequently been the culprit in several outbreaks and recalls associated with low-water activity foods (LWAF) due to contamination resulting from the environment, animals, or even the employees during pre-or post-processing. One such LWAF that has been associated with Salmonella spp. outbreaks is milk powder. Milk powders are used in a variety of products ranging from infant formula to confectionary goods. With their widespread use, improving their safety is imperative. A key step in improving their safety is to thoroughly investigate preventive controls related to milk powder processing. The Food Safety Modernization Act (FSMA) established the Preventive Controls for Human Foods Rule, which mandates that processors design and establish a food safety plan outlining any potential hazards and the steps they will take to ensure those hazards do not compromise the safety of the product. Presently, the dairy industry does not have an established kill-step for milk powders post-spray drying. Spray drying may reduce some microbial populations, however, desiccation(e.g., spray drying) is not considered a kill-step. The work outlined in this thesis aims to assist our dairy industry partners in establishing their own thermal process for milk powders post-spray drying. Moisture sorption isotherms of two milk powders (nonfat dry milk: NFDM and a milk protein concentrate with 85% protein content: MPC-85) were determined and showcased that the temperatures at which the isotherms were determined (23, 40, or 60°C) significantly affected both the adsorption and desorption isotherms (p<0.0001) for each powder. Secondly, the thermal inactivation kinetics of Salmonella spp. in both powders were evaluated at two different water activities (0.20 and 0.30) and three temperatures (75, 80, and 85°C) to determine the effect of water activity on the thermal resistance of a cocktail of Salmonella spp. The D-values of Salmonella were heavily influenced by the thermal treatment temperature (p< 0.0001), but not the water activity (p >0.05). The overall findings contained within this thesis provide valuable information about the thermal inactivation kinetics of Salmonella spp. to our partners in the dairy industry so that they may implement an appropriate thermal process for their milk powders.

The Relationship Between Performance, Body Composition, And Processing Yield In Broilers: A Systematic Review And Meta-Regression, Diego A. Martinez, Jordan T. Weil, Nawin Suesuttajit, Cole Alan Umberson, Abdullah Scott, Craig N. Coon

Poultry Science Faculty Publications and Presentations

This study aims to model the relationship among performance, whole body composition, and processing yield through meta-regression. Scientific papers found in Scopus and Google Scholar were included if they reported results and variability values of an actual experiment in the three mentioned groups of variables using a single broiler genetic line. Weighted mean effect sizes were determined with a random model, the risk of bias was determined, and heterogeneity was considered an indicator of usefulness. Meta-regressions considered the effect sizes of the response variable and the percent change in one or more variables as predictors. A 78-row database was built …

Development Of An Integrated Salt Cartridge-Reverse Electrodialysis (Red) Device To Increase Electrolyte Concentrations To Biomedical Devices, Efecan Pakkaner, Jessica L. Orton, Caroline G. Campbell, Jamie A. Hestekin, Christa N. Hestekin

Chemical Engineering Faculty Publications and Presentations

Emerging technologies in nanotechnology and biomedical engineering have led to an increase in the use of implantable biomedical devices. These devices are currently battery powered which often means they must be surgically replaced during a patient’s lifetime. Therefore, there is an important need for a power source that could provide continuous, stable power over a prolonged time. Reverse electrodialysis (RED) based biopower cells have been previously used to generate continuous power from physiologically relevant fluids; however, the low salinity gradient that exists within the body limited the performance of the biopower cell. In this study, a miniaturized RED biopower cell …

Broiler Vitamin Nutrition Guidelines, Nelson E. Ward

Proceedings of the Arkansas Nutrition Conference

In a time of record feed prices, nutritionists and production managers continue to look for opportunities to lower growout costs. Even though their contribution seldom exceeds 1-2% of feed costs, vitamins are not immune to this cost-crunching scrutiny. Furthermore, in late 2017, an unparalleled drop in global vitamin supply resulted in sharply increased prices, and in some cases, outright shortages. Such events have placed pressure on vitamin fortification levels in broiler feeds. Nutritionists decide on the fortification rates based on a variety of criteria – bird age, production phase, field experience, research trials, and so forth. The rapid change in …

Recent Findings On Phosphorus Digestibility Of Feed Ingredients In Broilers, Jay Hampton, Wenting Li, Franco Mussini, Katie Hilton, Janet Remus, Samuel J. Rochell

Proceedings of the Arkansas Nutrition Conference

Accurately meeting the dietary P needs of broilers is critical to ensure optimal bird performance, health, and welfare without causing undue environmental burdens associated with excess dietary P excretion. Phosphorus is commonly supplied in broiler diets from inorganic phosphates derived from non-renewable sources, but it has been estimated that peak P production will occur between 2030-2040 and that the global supply of P could be depleted within this century (Cordell and Drangert, 2009; Nest and Cordell, 2012). To address these concerns, inorganic P use in agriculture, including use in broiler diet formulation, requires careful stewardship. To this end, the dietary …

Reduction Of Protein Levels In Broiler Feed For Commercial Application – A German Case, Andreas Lemme, Kilian Fenske, Heiner Westendarp, Mathias Guhe, Elmar Rother

Proceedings of the Arkansas Nutrition Conference

A project was initiated to apply dietary CP reduction under commercial conditions. The main objective was to demonstrate and validate that dietary CP can be reduced without compromising broiler performance in a production system which is already rather efficient. In addition, we wanted to demonstrate the potential of dietary CP reduction on reducing N-excretions especially in the context of German revised regulations and monitoring attempts. Finally, as previous research suggested, few further aspects such as impact of dietary CP reduction on litter quality and quantity, footpad health, change of ingredient inclusion levels and related impact on sustainability impact factors were …

Application Of Simulation Modelling In Broiler Integration: Is It A Necessary Nutritional Tool?, Peter Chrystal

Proceedings of the Arkansas Nutrition Conference

Modelling techniques have been used in many industries globally to illustrate to others an understanding of the way processes work. Also, engineers and scientists employ models to predict the consequences of various courses of action. Simple pictorial models, such as the diagram of the chicken digestive system, are useful in education and may be more suitable than a photograph for the purposes of explaining how the animal converts ingested feed into components that can be assimilated, leaving residue that is excreted. Complex, predictive models seek to quantify a specified outcome based on defined inputs. Many attempts have been made over …

State Of The Art Pullet And Breeder Nutrition, Rick Van Emous

Proceedings of the Arkansas Nutrition Conference

During the last six to seven decades, broilers have been successfully selected for maximum growth and feed efficiency (Zuidhof et al., 2014). Modern fast-growing broilers reach a body weight of 2.0 to 2.5 kg in 35 to 45 days with an average daily growth of 55 or more gram a day (Augère-Granier, 2019). In a study with different broiler strains representative of broilers in 1957 or 1978 were compared with a broiler strain from 2005 (Zuidhof et al., 2014). The 2005 strain showed, over a 56-day growth period, a 4.6 times higher growth rate and a 50% lower feed conversion …

Broiler Breeder Males: Feed And Nutrition Discussion, Benton Hudson

Proceedings of the Arkansas Nutrition Conference

The methods for feeding broiler breeder males have evolved over recent decades, and further revisions will likely be needed as males become more challenging to manage. Since the introduction of separate male feeding systems in the early 1980’s, breeder males’ nutrient needs have been better managed via daily feed restriction. If a breeder operation can effectively produce, haul, and store a separate male diet, then it provides more options to use different nutrient levels or feed additives that are designed to enhance fertility. This article will outline the history of male-separate feeding and revisit nutritional research on fertility of broiler …

Amino Acids Were Not All Created Equal, Peter H. Selle, Shemil P. Macelline, Peter V. Chrystal, Sonia Y. Liu

Proceedings of the Arkansas Nutrition Conference

The contention that amino acids were not all created equal is based on the simple premise that intestinal uptakes of non-bound (synthetic, crystalline, feed-grade) amino acids are more rapid than their protein-bound counterparts. The post-enteral ramifications of this difference in bioequivalence are amplified in broiler chickens given their express growth rates and just one complication is the post-prandial oxidation of amino acids. The lack of bioequivalence between non-bound and protein-bound amino acids is a real obstacle to the development and adoption of reduced-crude protein diets that have the potential to promote sustainable chicken-meat production Thus, the purpose of this paper …

Recent Advancements On Calcium And Phosphorus Recommendations In Broilers, Justina Caldas, Marcelo Silva

Proceedings of the Arkansas Nutrition Conference

Calcium and phosphorus have been recognized essential nutrients in chickens since 1950’s (Lesson & Summers, 2001) playing important roles in bone development, among other functions. Calcium is assumed an inexpensive nutrient in animal least cost formulation while phosphorus is considered expensive and scant. The actual economic impact comes from the influence of calcium on phosphorus requirements, changes in phosphorus digestibility, buffer capacity, gut health, trace mineral absorption, fat digestibility, welfare traits, etc. Both minerals have important interactions to be considered during formulation. Few changes have been made over the past 20 years to calcium and phosphorus recommendations because these values …

Decoding The Gut Microbiome; Providing Solutions For Poultry Production, Nichoals Evans, Peter Karnezos, Curtis Novak, Diana Ayala, Emily Kimminau, Kay Russo

Proceedings of the Arkansas Nutrition Conference

The term “microbiome” refers to all the micro-organisms within a particular environment. In poultry, the microbiome is influenced by the barn, diet, and age of the birds and is believed to modulate immunity and inflammation. In the past, culture methods were primarily used to evaluate the presence of the various micro-organisms present in the microbiome; however, recent technological advances in genomic sequencing have revolutionized sample analysis. This technology has opened new avenues of research in poultry production and our understanding of the associated health challenges. Turkey cellulitis has been a major economic and health concern for many years. Traditional bacterial …

Application Of Nutritional Immunology In The Mitigation Of Economic And Production Losses In The Poultry Industry Associated With Food-Borne Pathogens, Coccidiosis, And Necrotic Enteritis., Shahna Fathima, Ramesh K. Selvaraj

Proceedings of the Arkansas Nutrition Conference

Sub-therapeutic doses of antibiotics were used in poultry production since the 1950s for improved production, prophylaxis, and animal welfare. Extensive and indiscriminatory use of antibiotics led to the emergence of antibiotic resistance in food-borne pathogens of public health significance (Jones & Ricke, 2003). As per the United States Centre for Disease Control, more than 2.8 million infections in 2019 were caused by multidrug resistant bacteria. Due to public health concerns, the use of antibiotic growth promoters in livestock production was prohibited by Sweden and Denmark in 1986 and 1998 respectively (Hammerum et al., 2007). The European Union banned the use …

A Dynamic Approach To Feed Formulation, Rob Gous

Proceedings of the Arkansas Nutrition Conference

The objective of this paper is to demonstrate that the opportunity cost of using fixed tables of nutrient requirements when formulating feeds for broilers can be considerable, and that nutritionists should consider a more dynamic approach to defining the lower bounds of amino acids in particular, in line with the objective of the business, which is to maximise profit. In order to do this, it is necessary to consider the potential growth rate of the broiler, the cost of feed and amount of feed consumed, and the income generated from the sale of product.

Transitioning To Net Energy: A Swine Story, John F. Patience

Proceedings of the Arkansas Nutrition Conference

Net energy (NE) is one member of the sequence of energy systems which also includes gross energy (GE), digestible energy (DE), and metabolizable energy (ME). It is perhaps the most sophisticated because it attempts to consider more components of the ingredient or diet which normally cannot be used by the pig for maintenance and/or productive purposes. Gross energy makes no such adjustment and therefore has little direct value in diet formulation. Digestible energy corrects for energy which is lost in the feces, and metabolizable energy also adjusts for energy lost in the urine. The data in Table …

Where Can We Go With Net Energy In Poultry?, Katie Hilton

Proceedings of the Arkansas Nutrition Conference

Metabolizable energy (ME) is the main system used globally in poultry industry for feed formulation and for evaluating feed ingredients and mixed feeds (de Boer and Bickel, 1988). ME is considered a reliable index of what is available to the bird for maintenance and production but is not a predictor of how efficiently the bird uses the available energy (MacLeod, 2000). The ME system developed by Hill and Anderson (1958) was reported to provide less variation in energy values for feed and ingredients than the Fraps PE system, but MacLeod (1994, 1997, 1999) suggest that the low variation …

Limestone Solubility: What Can You Do About It?, Wenting Li, Katie Hilton, Franco Mussini, Janet Remus

Proceedings of the Arkansas Nutrition Conference

Calcium (Ca) is essential for bone development mineralisation, as well as for eggshell formation, muscle and neural functions. Depending on the type of diets (veggie vs. non-veggie), species (turkey, chicken, duck), and age of animal (young vs. adult), limestone can contribute up to 95% total Ca in the final diet, with more Ca from limestone for younger animals with veggie diet. Despite the significant contribution of limestone to total diet Ca levels, the bioavailability variation of limestone Ca is rarely considered. Phosphorus (P) is a limited resource and is the third most costly ingredient in poultry …

Geography, Niches, And Transportation Influence Bovine Respiratory Microbiome And Health, Jianmin Chai, Xinting Liu, Hunter Usdrowski, Feilong Deng, Ying Li, Jiangchao Zhao

Animal Science Faculty Publications and Presentations

Bovine respiratory disease (BRD), one of the most common and infectious diseases in the beef industry, is associated with the respiratory microbiome and stressors of transportation. The impacts of the bovine respiratory microbiota on health and disease across different geographic locations and sampling niches are poorly understood, resulting in difficult identification of BRD causes. In this study, we explored the effects of geography and niches on the bovine respiratory microbiome and its function by re-analyzing published metagenomic datasets and estimated the main opportunistic pathogens that changed after transportation. The results showed that diversity, composition, structure, and function of the bovine …

Evaluation Of Potassium Borate As A Volatility-Reducing Agent For Dicamba, Mason Castner, Jason K. Norsworthy, Trenton Roberts

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Dicamba was labeled in dicamba-resistant cotton (Gossypium hirsutum L.) and soybean [Glycine max (L.) Merr.] in 2017, resulting in a record number of off-target complaints. To address off-target movement via volatilization, experiments were conducted to evaluate the effectiveness of potassium tetraborate tetrahydrate (KBo) as a volatility-reducing agent (VRA) with dicamba. Low-tunnel experiments examined: (1) whether KBo functions as a dicamba VRA, (2) the relationship between KBo concentration and dicamba volatilization, (3) the effectiveness of KBo compared with potassium acetate as a VRA, and (4) the impact of KBo on dicamba volatilization with and without glufosinate. In a large-scale trial (0.4-ha …

Pgc-1Α Overexpression Is Not Sufficient To Mitigate Cancer Cachexia In Either Male Or Female Mice, Francielly Morena Da Silva, Megan Rosa-Caldwell, Eleanor R. Schrems, Lauren Martinez, Madeline G. Amos, Seongkyun Lim, Ana Regina Cabrera, Jacob L. Brown, Tyrone A. Washington, Nicholas P. Greene

Health, Human Performance and Recreation Faculty Publications and Presentations

Cancer cachexia (CC) accounts for 20%–40% of cancer-related deaths. Mitochondrial aberrations have been shown to precede muscle atrophy in different atrophy models, including cancer. Therefore, this study investigated potential protection from the cachectic phenotype through overexpression of peroxisome proliferator-activated receptor γ coactivator-1 α (PGC-1α). First, to establish potential of mitochondria-based approaches we showed that the mitochondrial antioxidant MitoTEMPO (MitoT) attenuates myotube atrophy induced by Lewis lung carcinoma (LLC) cell conditioned media. Next, cachexia was induced in muscle-specific PGC-1α overexpressing (MCK-PCG1α) or wildtype (WT) littermate mice by LLC implantation. MCK-PCG1α did not protect LLC-induced muscle mass loss. In plantaris, Atrogin mRNA …

Summaries Of Arkansas Cotton Research 2021, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

While the basic growth and development of the cotton plant have not changed significantly in recent history, the business of cotton production is ever-changing. The last two years have seen us plant a crop just about as late as we thought possible, yet extended favorable conditions at season's end have been our salvation, helping to lead us to record yields. The economic environment over the last few years has been such that farmers need to produce record or near-record yields to advance. Unfortunately, production levels at the state yield average barely cover out-of-pocket expenses.

Great uncertainties exist for the upcoming …

Using Poultry To Enhance Food Security In Stann Creek, Belize, Emily Spatz

Poultry Science Undergraduate Honors Theses

Food insecurity is a growing issue in developing and developed countries alike, and in countries like Belize, the prevalence of hunger has soared in recent years. Not having access to diets with sufficient calories and nutrients leads to a host of developmental issues, including stunting and cognitive delays. The purpose of this work was to create an all-encompassing manual for small-scale broiler production, with the intention of another honors student overseeing the implementation of the ideas set forth in the manual, in order to provide nourishment for the students and staff of a school in Belize. After determining that the …

Perception Of Illness And Its Association With Willingness To Adhere To Treatment In Chronic Hepatitis C Patients With New Diagnosis Of Nonalcoholic Fatty Liver Disease, Carmel Kruse

The Eleanor Mann School of Nursing DNP Capstone Projects

Chronic liver disease is becoming the most common cause of mortality, morbidity, and utilization of healthcare services globally. Both nonalcoholic fatty liver disease (NAFLD) and chronic hepatitis C (CHC) are frequent causes of chronic liver disease. Recently, there have been significant findings on the relationship between NAFLD and CHC; patients with CHC have a higher incidence of NAFLD. The introduction of direct-acting antivirals has cured more CHC patients than ever before. Unfortunately, the current interventions for NAFLD requiring behavioral change remain ineffective. Patient’s ability to follow advice depends largely on their health beliefs; therefore, understanding their illness perception is an …

Assessing Consumer Demand, Producer Profitability, And The Environmental Impacts Of Conservation Agriculture Adoption In Sub-Saharan Africa, Willy Byamungu Mulimbi

Graduate Theses and Dissertations

This dissertation explores three aspects of conservation agriculture (CA) in the Sub-Saharan African region (SSA). The first article examines the demand side of CA and explores whether urban maize (Zea mays L.) consumers in the Democratic Republic of the Congo (DRC) would be willing to pay a premium for CA-produced maize flour. The second article estimates the effects CA provides to adopters and their farms in smallholder farming systems in the DRC, focusing on changes in soil properties and cowpea (Vigna unguiculata) yields. The final article uses a Life Cycle Assessment (LCA) approach to monetize the environmental impacts of adopting …

Mitochondrial Dna Diversity, Parasite And Pathogen Occurrence, And A Potential Disease Vector In Managed And Unmanaged Honey Bee, Apis Mellifera L. Populations, Dylan Cleary

Graduate Theses and Dissertations

The western honey bee, Apis mellifera L., is a globally important pollinator plagued by several harmful stressors impacting colony health and survival. At least eight A. mellifera subspecies were imported and continue to be the genetic ancestors of U.S. honey bee populations today. Successive genetic bottle-neck events have led to reduced genetic diversity in U.S. honey bees. First, the subset of subspecies imported into the U.S. represents only a third of A. mellifera subspecies. Next, the parasitic varroa mite reduced managed and feral populations. Third, ongoing breeding practices have selected for traits from a single genetic lineage and bred from …

A Review Of Heat Stress In Chickens. Part Ii: Insights Into Protein And Energy Utilization And Feeding, Jean-Remi Teyssier, Giorgio Brugaletta, Federico Sirri, Sami Dridi, Samuel J. Rochell

Poultry Science Faculty Publications and Presentations

With the growing global demand for animal protein and rising temperatures caused by climate change, heat stress (HS) is one of the main emerging environmental challenges for the poultry industry. Commercially-reared birds are particularly sensitive to hot temperatures, so adopting production systems that mitigate the adverse effects of HS on bird performance is essential and requires a holistic approach. Feeding and nutrition can play important roles in limiting the heat load on birds; therefore, this review aims to describe the effects of HS on feed intake (FI) and nutrient digestibility and to highlight feeding strategies and nutritional solutions to potentially …

Histomonosis In Turkeys – Factors Influencing Transmission, Pathogenesis, And The Search For Prophylactic Or Therapeutic Compounds, Thainá Landim De Barros

Graduate Theses and Dissertations

The incidence of histomonosis has been increasing in poultry since the ban of prophylactic and therapeutic compounds. Histomonosis is caused by the protozoa Histomonas meleagridis. The objective of this dissertation was to investigate factors impacting the pathogenesis and transmission of histomonosis and to evaluate compounds that could potentially prevent or reduce the severity of histomonosis in turkeys. In the first study, the effect of sodium chlorate and sodium nitrate on reducing histomonads growth was tested in vitro and added to a basal turkey diet. A decrease in the growth of histomonads in vitro was observed, but no in vivo effect …

Sex Differences In Cancer Cachexia And A Novel Mitochondrial Target For Cancer-Induced Muscle Wasting, Seongkyun Lim

Graduate Theses and Dissertations

Cancer cachexia (CC) is a devastating wasting syndrome characterized by marked weight loss including skeletal muscle atrophy that affects approximately 80% of cancer patients. Current therapeutic treatments including pharmacological and nutritional intervention are insufficient to prevent or reverse it. Prior studies demonstrated lower muscle mass, impaired muscle function, and mitochondrial health in the development of CC. Specifically, mitochondrial fusion protein, Optic atrophy 1 (OPA1) which plays a significant role in skeletal muscle health, is suppressed in the development of CC. Furthermore, most pre-clinical CC studies were mainly focused on males although there are distinct phenotypical differences in skeletal muscle between …

Characterization Of Blueberry (Poly)Phenolics In Foods, Their Metabolites In Human Biological Samples And Anti-Inflammatory Effects Of Phytochemicals In Vitro, Laura Felgus-Lavefve

Graduate Theses and Dissertations

Blueberries’ popularity is in part supported by the health benefits associated with their consumption. Research has strongly focused on their polyphenol and anthocyanin concentration, one of the highest amongst commonly consumed foods. Polyphenols have demonstrated antioxidant effects in vitro, and consuming polyphenol-rich foods is associated with protective effects towards non-communicable diseases, but their direct bioactivity in the human body remains to be fully understood. It is estimated that about 90 to 95% of the initial polyphenol intake reaches the colon without being absorbed. Instead, phenolic compounds go through extensive biotransformations, consisting of catabolism into smaller compounds mostly carried out by …

Modeling The Impact And Accelerating The Process Of Transitioning To A Sustainable Healthy Diet Through Decision Support Systems, Prince Agyemang

Graduate Theses and Dissertations

Food production and consumption are essential in human existence, yet they are implicated in the high occurrences of preventable chronic diseases and environmental degradation. Although healthy food may not necessarily be sustainable and vice versa, there is an opportunity to make our food both healthy and sustainable. Attempts have been made to conceptualize how sustainable healthy food may be produced and consumed; however, available data suggest a rise in the prevalence of health-related and negative environmental consequences of our food supply. Thus, the transition from conceptual frameworks to implementing these concepts has not always been effective. This paper explores the …