Digital Commons Network^™

Investigation Of Nanodiamonds With Si-V Defect Centers For Applications In Fluorescence-Based Sensing And Drug Delivery, Sonal Singh

All ETDs from UAB

Fluorescent Nanodiamond (FND) offers a promising platform for many therapeutic applications involving drug and gene delivery, optical agents for bioimaging and labeling, and molecular detection. This is due to the potential to incorporate photostable luminescent vacancy-related defects into sub-10 nm diamond crystals which are biologically compatible and easy to functionalize. Moreover, the development of future nanoscale devices and arrays based on nanodiamonds (NDs) will require precise spatial control. Hence, the direct placement and manipulation (control of size, shape and chemical functionality) of brightly fluorescent sub-10 nm NDs offers potential for highly localized /specific imaging and sensing, while also providing the …

The Pleasure Of Memory Formation: Dna Methylation Regulates Associative Reward Learning, Roxie Lockhart

Inquiro (undergraduate research journal)

pp. 13-14

Section 1: Bio-Medical/Bioengineering, Asme Ectc Coordinating Committee

Early Career Technical Journal (ECTJ)

Proceedings of the ASME District F Early Career Technical Conference, November 2-3, 2013. School of Engineering, University of Alabama at Birmingham, Birmingham, Alabama

pp. 1-38

Section 5: Numerical Methods, Asme Ectc Coordinating Committee

Early Career Technical Journal (ECTJ)

Proceedings of the ASME District F Early Career Technical Conference, November 2-3, 2013. School of Engineering, University of Alabama at Birmingham, Birmingham, Alabama

pp. 153-190

Section 6: Fluids Engineering, Asme Ectc Coordinating Committee

Early Career Technical Journal (ECTJ)

Proceedings of the ASME District F Early Career Technical Conference, November 2-3, 2013. School of Engineering, University of Alabama at Birmingham, Birmingham, Alabama

pp. 191-222

Design And Testing Of A Center-Of-Pressure Oscillation Measurement Device For Assessment Of Postural Control Recovery, Yong Zhu, Adetayo Faminu, Li Li

Early Career Technical Journal (ECTJ)

Proceedings of the ASME District F Early Career Technical Conference, November 2-3, 2013. School of Engineering, University of Alabama at Birmingham, Birmingham, Alabama

Section 1: Bio-Medical/Bioengineering; pp. 14-19

Design And Eeg-Based Control Of A Bio-Inspired Ankle Foot Orthosis, Daniel K. Olender, Yong Zhu

Early Career Technical Journal (ECTJ)

Proceedings of the ASME District F Early Career Technical Conference, November 2-3, 2013. School of Engineering, University of Alabama at Birmingham, Birmingham, Alabama

Section 1: Bio-Medical/Bioengineering; pp. 7-13

Determination Of Particle Aerodynamic Size Distribution And Viability Of Aerosolized H1n1 Virus, Mohammed Ali, Essam A. Ibrahim

Early Career Technical Journal (ECTJ)

Proceedings of the ASME District F Early Career Technical Conference, November 2-3, 2013. School of Engineering, University of Alabama at Birmingham, Birmingham, Alabama

Section 1: Bio-Medical/Bioengineering; pp. 20-26

Counter-Balancing Mechanism For Improving Independence When Using An Exoskeleton, Drew A. Lathan, Beth A. Todd

Early Career Technical Journal (ECTJ)

Proceedings of the ASME District F Early Career Technical Conference, November 2-3, 2013. School of Engineering, University of Alabama at Birmingham, Birmingham, Alabama

Section 1: Bio-Medical/Bioengineering; pp. 34-37

Asme District F Ectc Proceedings / Early Career Technical Journal (Complete Issue), Asme Ectc Coordinating Committee

Early Career Technical Journal (ECTJ)

Proceedings of the ASME District F Early Career Technical Conference, November 2-3, 2013. School of Engineering, University of Alabama at Birmingham, Birmingham, Alabama

Using Compact Finite-Difference Scheme Along With The Immersed-Boundary Method, Chi Zhu, Guibo Li, Haoxiang Luo

Early Career Technical Journal (ECTJ)

Proceedings of the ASME District F Early Career Technical Conference, November 2-3, 2013. School of Engineering, University of Alabama at Birmingham, Birmingham, Alabama

Section 5: Numerical Methods; pp. 186-190

Performance Of A Hummingbird During Hovering Flight, Jialei Song, Haoxiang Luo, Tyson L. Hedrick

Early Career Technical Journal (ECTJ)

Proceedings of the ASME District F Early Career Technical Conference, November 2-3, 2013. School of Engineering, University of Alabama at Birmingham, Birmingham, Alabama

Section 6: Fluids Engineering; pp. 201-206

Cfd Modeling Of The Vocal Fold Vibration For Evoked Rabbit Phonation, Siyuan Chang, Fangbao Tian, Haoxiang Luo, James F. Doyle, Tsuyoshi Kojima, Bernard Rousseau

Early Career Technical Journal (ECTJ)

Proceedings of the ASME District F Early Career Technical Conference, November 2-3, 2013. School of Engineering, University of Alabama at Birmingham, Birmingham, Alabama

Section 6: Fluids Engineering; pp. 207-212

Spider Web Topology: Parameterization And Analysis, Pankaj Rajput, Giovanni Di Ilio, Ronald Diaz

Early Career Technical Journal (ECTJ)

Proceedings of the ASME District F Early Career Technical Conference, November 2-3, 2013. School of Engineering, University of Alabama at Birmingham, Birmingham, Alabama

Section 5: Numerical Methods; pp. 172-179

The Phenotypic Expression Of Usp14 Deficiency Is Dependent Upon Genetic Background, Andrea Gail Marshall

All ETDs from UAB

The ubiquitin proteasome system (UPS) plays a critical role in regulating a diversity of cellular processes that are essential to neuronal function, such as synaptic transmission, axon guidance, and neurite outgrowth. Dysfunctions of the UPS are linked with many neurodegenerative disorders, including motor neuron diseases like spinal muscular atrophy (SMA) and amyotrophic lateral sclerosis (ALS), although it is not known how UPS dysfunction contributes to disease pathology. The proteasome, an approximate 2.5 megadalton protein complex comprised of a 20S core particle and 19S regulatory particle, is the site of ubiquitin-mediated proteolysis in the cell. Recent studies have linked ubiquitin specific …

In Vitro Prevascularization Of 3d Tissue Constructs, Lauren Marshall

All ETDs from UAB

There is a need for more effective therapies to combat breast cancer. One reason for the inefficiency of therapeutics is that testing technologies currently employed in drug discovery and development are not reliable when transitioning drugs into human clinical trials. To supplement the currently used in vitro cell culture studies and in vivo animal studies, an in vitro three-dimensional breast tumor system has been suggested with integration of human cells, hydrogel biomaterials, 3D architecture, and endothelialized microchannels. Therefore, the objective of the thesis project, as an initial step in fabrication of the supplemental system for drug testing, is to fabricate …

Cd5 Regulation Of T Helper Cell Differentiation And Cytokine Signaling, Donald J. Mcguire

All ETDs from UAB

CD5 is expressed on T and B1a cells and is an important regulator of cell survival and activation. Engagement of CD5 promotes cell survival through the activation of the serine threonine kinase CK2. Mice with genetic abrogation of CD5 binding to CK2 (CD5∆CK2bd) exhibited reduced severity of experimental autoimmune encephalomyelitis that was associated with increased activation induced cell death. In addition to inhibiting cell death, CD5 activation of CK2 significantly enhances Th17 differentiation. Loss of CD5-CK2 signaling reduces the generation of Th17 cells despite an unexpected increase in pSTAT3 levels. The mechanisms of CD5's enhancement of Th17 differentiation have not …

Intrinsic And Extrinsic Factors Required For Cd4 T Memory Cell Formation And Function, Sarah B. Mollo

All ETDs from UAB

CD4 T cells are central to the organization of the immune response through the secretion of cytokines that recruit and activate other immune cells. Following infection and pathogen control, the majority of the effector CD4 T cells undergo apoptosis, leaving a subset that persists and gives rise to the memory T cell pool. Upon encountering the same pathogen, memory CD4 T cells respond rapidly, providing enhanced protection from re-infection. What determines which effector CD4 T cells will survive is unclear; however, there are a number of factors both intrinsic to the cell as well as external signals from the environment …

Frequency Of Exercise Training's Effect On Psychological Outcomes In Older Women, William Neumeier

All ETDs from UAB

Objectives: Exercise training by older adults has demonstrated many health benefits, including improved cognition and psychological well-being. The optimal frequency of combined aerobic exercise training (AET) and resistance exercise training (RET) is unknown. The purpose of this study was to examine whether exercise improves body image, esteem, depression, and mood, among older women, and whether improvements differ by frequency of exercise. Methods: Post-menopausal women (N = 64) over the age of 60 (64.79 ± 3.74) were randomly assigned to one of three groups. Group 1 completed two exercise sessions (1 AET, 1 RET) per week, Group 2 completed four sessions …

An Investigation Of The Sublethal Effects Of Carbon Dioxide On The Common Sea Urchin Lytechinus Variegatus And The Carbonate Chemistry Of Its Nearshore Habitat, Roberta Challener

All ETDs from UAB

Increases in CO2 concentrations, whether from accumulation of metabolic waste under intensive culturing conditions or from increases in atmospheric levels, lead to decreases in seawater pH and carbonate saturation states. Lytechinus variegatus survive chronic exposure to hypercapnic conditions (pH 7.4 -7.8, partial pressure of CO2 (pCO2) 1738-4290 μatm) in synthetic seawater, yet aspects of growth and development are affected. Rates of embryonic and larval development were delayed and larval size and arm length reduced under high pCO2 conditions. Fecal production rates were higher and ash absorption efficiency (%) was lower in individuals exposed to hypercapnic conditions, suggesting that the ability …

Identification Of Staphylococcus Aureus Sortase A Inhibitors As Potential Antibacterial Agents, Bala Chandra Chenna

All ETDs from UAB

Staphylococcus aureus (S. aureus) is the causative organism for a wide range of infectious diseases from superficial skin infections to dangerous bacteremias. Six pathogens named ESKAPE pathogens are responsible for majority of antibiotic resistant bacterial infections. MRSA is the single most prevalent ESKAPE pathogen. Many of the nosocomial MRSA strains are multi-drug resistant, and even methicillin-sensitive strains can be deadly. S. aureus has recently taken on a new role in causing an escalating number of community-acquired infections in non-hospitalized persons without predisposing risk factors. Due to the increase in the number of infections caused by multi-drug resistant S. aureus and …

Structure-Function Analysis Of Tyrosyl-Dna Phosphodiesterase I, Evan Comeaux

All ETDs from UAB

Tyrosyl-DNA phosphodiesterase I (Tdp1) functions to remove 3'phospho-DNA adducts, such as eukaryotic DNA Topoisomerase I (Top1) covalently bound to the DNA by a 3'phospho-tyrosyl bond. As member of the Phospholipase D superfamily Tdp1 contains two HxK(n)N motifs, each of which provide a catalytic histidine: one functioning as a nucleophile to bind the 3'phosphate and the other as a general acid/base to hydrolyze Tdp1's 3'phospho-histidyl bond. Mutation of the general acid/base catalytic histidine (Hisgab) to arginine has been implicated in the autosomal recessive neurodegenerative disease SCAN1. Further study of the yeast enzyme revealed that substitution of Hisgab in yeast Tdp1 to …

Tgf-Ss And Erg In Intervertebral Disc Development, Megan Karen Cox

All ETDs from UAB

The intervertebral disc (IVD) is a fibrocartilaginous tissue that acts as a shock absorber between adjacent vertebrae in the spinal column. It is comprised of two parts, the inner jelly-like nucleus pulposus (NP) and the outer more fibrous annulus fibrosus (AF). Most IVD research has focused on the development of the NP, which is derived from condensations of the notochord involving sonic hedgehog (Shh) signaling. In contrast, very little is known about the molecular mechanisms underlying the specific development of the AF of the IVD. The AF is derived from the sclerotome, which is formed by the ventral half of …

Utilizing A Hydroxyapatite-Binding Domain To Deliver Bone Regenerative Molecules To Hydroxyapatite And Allograft Bone To Enhance Osteogenesis, Bonnie Kathleen Culpepper

All ETDs from UAB

Hydroxyapatite (HA) makes up the mineral component of bone, and synthetic HA is commonly used to coat metal implants for use in dentistry and orthopedics. Additionally, allograft and synthetic HA are common alternatives to autograft bone, to circumvent the complications associated with a second surgery site. While both HA and allograft are known to be osteoconductive, osteoinductivity is limited. In this work, we developed methods for anchoring osteoinductive factors onto multiple types of HA biomaterials and human allograft bone, with the goal of enhancing their osteoregenerative potential. Specifically, a HA-binding domain, heptaglutamate (E7), modeled on bone-binding domains within native proteins, …

An Investigation Of Trem-Like Transcript 2 Expression, Kimberly Anne Thomas

All ETDs from UAB

Acute inflammation is a necessary component for the clearance of pathogens. Cells at the site of infection utilize highly conserved receptors to both recognize microbes and induce the production of pro-inflammatory stimuli. These stimuli set off a chain reaction that includes localized vasodilation, leukocyte infiltration, and tissue destruction, collectively known as inflammation. Tightly controlled, this process is protective, and can compartmentalize the damage to a defined area, but when dysregulated can be catastrophic, inducing chronic inflammation and even death. Families of innate receptors can modulate inflammatory processes, and in doing so, increase or decrease their severity, as well as tailor …

Influence Of Implant Angulation On The Fracture Resistance Of Zirconia Abutments, Shreedevi Thulasidas

All ETDs from UAB

Zirconia-based implant abutments are widely used in prosthetic dentistry. Custom made abutments are utilized to correct for non-ideal implant positions and can successfully accommodate the morphologic requirements of the overlying crown. It was hypothesized that there will be difference in fracture strength and fracture mode of artificially aged zirconia implant abutments supported by implants at varying angulation, when load is applied to failure on overlying zirconia crowns. The implant-abutment-crown assemblies were subjected to steam autoclaving to induce some degree of phase transformation from tetragonal to weaker monoclinic form and to thermo-cycling to artificially age the samples. The effects of loading …

Glioma-Vasculature Interactions, Stacey Watkins

All ETDs from UAB

Malignant gliomas are highly invasive brain tumors, which lack effective treatment options. To date, the standard therapy includes surgical debulking followed by chemotherapy and radiation. Despite efforts to eradicate the disease, the median patient survival time is only 14 months. In an attempt to discover novel therapies, research has focused on understanding the heterogeneous biological mechanisms employed by these cells. Uniquely, they do not metastasize through the vasculature or lymphatics like other solid cancers but alternatively, glioma cells utilize the abluminal surface of the vasculature to guide invasion and migration throughout the brain. The intimate association between glioma cells and …

Hur Regulation In Central Nervous System Disorders, Crystal G. Wheeler

All ETDs from UAB

In inflammatory diseases of the central nervous system (CNS), astrocytes play a major role in the systemic response to disease by either enhancing or limiting the inflammatory response through secretion of growth factors or cytokines. HuR RNA binding protein regulates many genes involved in inflammation. Inflammatory diseases such as brain tumors, Amylotrophic Lateral Sclerosis (ALS), and Multiple Sclerosis (MS), have been linked to HuR, by our laboratory and others. To determine whether astrocytic overexpression of HuR would regulate inflammation in diseases of the CNS, we designed a transgenic mouse in which the HuR gene is overexpressed. In the first study, …

Visible Learning In A Reggio Emilia Inspired Kindergarten: Parents' Descriptions Of Documentation, Melissa Gibbons Whetstone

All ETDs from UAB

The purpose of this qualitative study was to reveal multiple perspectives and thoughts of seven parents and a family member in a Reggio Emilia inspired kindergarten classroom in a southeastern state of the United States regarding pedagogical documentation, an essential tool for teaching practice and reflection. It served to make knowledge construction visible through children's interpretive concrete work. Throughout the study, pedagogical documentation was defined as both a learning process as well as the products of early childhood content. The content were concrete materials in the form of notes, photographs, audio and video recordings, computer graphics, and examples of children's …

Neural Mechanisms Supporting The Learning-Related Emotional Response To A Threat, Kimberly H. Wood

All ETDs from UAB

Successful regulation of the emotional response to a threat allows one to react more effectively under threatening conditions. The prefrontal cortex (PFC) and amygdala are key brain regions that mediate the regulation and expression of emotion. We employed Pavlovian fear conditioning to investigate the neural mechanisms that influence the emotional response to a threat. These procedures were designed to investigate conditioned diminution of the unconditioned response (UCR). The specific aims were to better understand the role of associative learning, expectation, controllability, and predictability in modulating UCR expression. This project employed functional magnetic resonance imaging (fMRI) to assess the magnitude of …