Digital Commons Network^™

Structural And Functional Analysis Of Parameters Governing Tankyrase-1 Interaction With Telomeric Repeat-Binding Factor 1 And Gdp-Mannose 4,6-Dehydratase., Travis Eisemann, Marie-France Langelier, John M. Pascal

Department of Biochemistry and Molecular Biology Faculty Papers

Human tankyrase-1 (TNKS) is a member of the poly(ADPribose) polymerase (PARP) superfamily of proteins that posttranslationally modify themselves and target proteins with ADP-ribose (termed PARylation). The TNKS ankyrin repeat domain mediates interactions with a growing number of structurally and functionally diverse binding partners, linking TNKS activity to multiple critical cell processes, including Wnt signaling, Golgi trafficking, and telomere maintenance. However, some binding partners can engage TNKS without being modified, suggesting that separate parameters influence TNKS interaction and PARylation. Here, we present an analysis of the sequence and structural features governing TNKS interactions with two model binding partners: The PARylated partner …

Changes In Body Mass Index And Atherosclerotic Disease Risk Score After Switching From Tenofovir Disoproxil Fumarate To Tenofovir Alafenamide., Jason J. Schafer, Kaitlin N. Sassa, Jaclyn R. O'Connor, Ayako Shimada, Scott W. Keith, Joseph A. Desimone

College of Pharmacy Faculty Papers

Background: Switching from tenofovir disoproxil fumarate (TDF) to tenofovir alafenamide (TAF)-containing antiretroviral therapy (ART) can improve renal function and bone mineral density in people with human immunodeficiency virus (PWH). The switch can also negatively influence cholesterol, but changes in body mass index (BMI) and atherosclerotic cardiovascular disease (ASCVD) risk are unknown.

Methods: This retrospective observational study evaluated BMI and ASCVD risk score changes in virologically suppressed PWH who switched from TDF to TAF without switching other ART regimen components. Adults on TDF for ≥1 year with 2 consecutive HIV ribonucleic acid values/mL before a TAF switch were included. Body weight, …

Clinical, Cytogenetic, And Molecular Findings In Two Cases Of Variant T(8;21) Acute Myeloid Leukemia (Aml)., Lindsay Wilde, Jillian Cooper, Zi-Xuan Wang, Jinglan Liu

Department of Medical Oncology Faculty Papers

t(8;21)(q22;q22) is present in ~5–10% of patients with de novo acute myeloid leukemia (AML) and is associated with a better overall prognosis. Variants of the t(8;21) have been described in the literature, however, their clinical and prognostic significance has not been well-characterized. Molecular profiling of these cases has not previously been reported but may be useful in better defining the prognosis of this subset of patients. We present two cases of variant t(8;21) AML including clinical, cytogenetic, and molecular data.

Mitochondrial Calcium Exchange Links Metabolism With The Epigenome To Control Cellular Differentiation., Alyssa A. Lombardi, Andrew A. Gibb, Ehtesham Arif, Devin W. Kolmetzky, Dhanendra Tomar, Timothy S. Luongo, Pooja Jadiya, Emma K. Murray, Pawel K. Lorkiewicz, György Hajnóczky, Elizabeth Murphy, Zoltan P. Arany, Daniel P. Kelly, Kenneth B. Margulies, Bradford G. Hill, John W. Elrod

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Fibroblast to myofibroblast differentiation is crucial for the initial healing response but excessive myofibroblast activation leads to pathological fibrosis. Therefore, it is imperative to understand the mechanisms underlying myofibroblast formation. Here we report that mitochondrial calcium (_mCa²⁺) signaling is a regulatory mechanism in myofibroblast differentiation and fibrosis. We demonstrate that fibrotic signaling alters gating of the mitochondrial calcium uniporter (mtCU) in a MICU1-dependent fashion to reduce _mCa²⁺ uptake and induce coordinated changes in metabolism, i.e., increased glycolysis feeding anabolic pathways and glutaminolysis yielding increased α-ketoglutarate (αKG) bioavailability. _mCa²⁺-dependent metabolic reprogramming leads …

Validation Of A Miniaturized Permeability Assay Compatible With Crispr-Mediated Genome-Wide Screen., Claire Simonneau, Junning Yang, Xianguo Kong, Robert Kilker, Leonard Edelstein, Paolo Fortina, Eric R. Londin, Arie Horowitz

Cardeza Foundation for Hematologic Research

The impermeability of the luminal endothelial cell monolayer is crucial for the normal performance of the vascular and lymphatic systems. A key to this function is the integrity of the monolayer's intercellular junctions. The known repertoire of junction-regulating genes is incomplete. Current permeability assays are incompatible with high-throughput genome-wide screens that could identify these genes. To overcome these limitations, we designed a new permeability assay that consists of cell monolayers grown on ~150 μm microcarriers (MCs). Each MC functions as a miniature individual assay of permeability (MAP). We demonstrate that false-positive results can be minimized, and that MAP sensitivity to …

Association Of Β-Amyloid Burden With Sleep Dysfunction And Cognitive Impairment In Elderly Individuals With Cognitive Disorders., Jason C. You, Erica Jones, Devon E. Cross, Abigail C. Lyon, Hyunseung Kang, Andrew B. Newberg, Carol F. Lippa

Department of Neurology Faculty Papers

Importance: Evidence shows that sleep dysfunction and β-amyloid (Aβ) deposition work synergistically to impair brain function in individuals with normal cognition, increasing the risk of developing dementia later in life. However, whether Aβ continues to play an integral role in sleep dysfunction after the onset of cognitive decline in individuals with dementia is unclear.

Objective: To determine whether Aβ deposition in the brain is associated with subjective measures of sleep quality and cognition in elderly individuals with cognitive disorders.

Design, Setting, and Participants: A nested survey study was conducted at the Cognitive Disorders and Comprehensive Alzheimer Disease Center of Thomas …

Arming Of The U.S. Army During War 1861, Jessica Colfer

Lesson Plans

Grade Level: 9-12

Lesson Length: 60 minutes

Learning Objectives:

• The student will be able to identify the armament of the Union army at the beginning of the Civil War.
• The student will consider the preparedness of the Union and Confederate armies.
• The student compare and contrast prior knowledge about the Civil War to interpret historical documents.
• The student will be able to analyze and interpret a primary document.

The Election Of 1860 And The Secession Of The South, Jessica Colfer

Lesson Plans

Grade Level: 9-12

Lesson Length: 80 minutes

Learning Objectives:

• Students will be able to analyze primary documents and identify the relation between student attendance and the political and societal context of the time.
• Students will be able to analyze and apply their prior knowledge to interpret the perspectives of those during the outbreak of the Civil War.
• Students will be able to identify the primary causes of South Carolina’s secession from the Union.

Nurses Alumni Association Bulletin, Fall 2019, Susan Albrecht Curcio

Nursing Alumni Bulletins

2019–2020 Meeting Dates Calendar

2020 Annual Luncheon and Meeting Notice

Officers for 2019–2020

President’s Message

Treasurer’s Report

Resume of Minutes

Office News

Committee Reports

• Social
• Relief Trust Fund
• Central Pennsylvania Satellite Group
• Scholarship
• By-Laws
• Nominating
• Bulletin

Jefferson Office of Alumni Relations

Annual Giving

In Honor of and in Memory of

Janet C. Hindson Award

• Janet C. Hindson Award Winner

Pearls of Wisdom

The History of the Jefferson Cap

A Nurses’ Cape Finds a Home

Happy Birthday – to Be 80 or More

Flower Thank You Notes

Annual Luncheon

• Photos
• Annual Luncheon Comments
• 1970 – 50th Anniversary Class List

In Memoriam …

Scott Memorial Library, Center For Teaching And Learning & Scott Memorial Library

Electronic Handouts

Guide to educational, research and clinical information services available for Jeffersonians at Scott Memorial Library.

Annual Report 2018-2019, Elizabeth D'Angel

Annual Reports & Administrative Documents

No abstract provided.

Jefferson Digital Commons Quarterly Report: July-September 2019, Kelsey Duinkerken, Msi, James Copeland, Libt, Larissa Gordon, Mslis, Med, Ma

Jefferson Digital Commons Reports

This quarterly report includes:

• Articles
• Conferences
• From the Archives
• Grand Rounds and Lectures
• Journals and Newsletters
• Master of Population Health Capstone Presentations
• Master of Public Health Capstone Presentations
• Posters
• What People are Saying About the Jefferson Digital Commons

The Scott Memorial Library Quasquicentennial: Celebrating 125 Years Of The Jefferson Library (1894-2019), Kelsey Duinkerken, Msi

Library Posters

The Early Years

(1894-1930s)

The Modern Library

(1960s-1970s)

The Computer Age

(1980s-2019)

Which Student Characteristics Are Most Important In Determining Clinical Honors In Clerkships? A Teaching Ward Attending Perspective., Lauren Nicholas Herrera, Ryan Khodadadi, Erinn Schmit, James Willig, Andrew Hoellein, Christopher Knudson, Karen Law, Nina Mingioni, Katherine Walsh, Carlos Estrada, Winter Williams

Division of Internal Medicine Faculty Papers & Presentations

PURPOSE: To explore faculty perspectives on which characteristics of high-performing clerkship students are most important when determining an honors or top grade designation for clinical performance.

METHOD: In 2016-2017, the authors surveyed faculty (teaching ward attendings) for internal medicine clerkships and 1 pediatrics clerkship in inpatient settings at 5 U.S. academic medical centers. Survey items were framed around competencies, 24 student characteristics, and attitudes toward evaluation. Factor analysis examined constructs defining high-performing students.

RESULTS: Of 516 faculty invited, 319 (62%) responded. The top 5 characteristics as rated by respondents were taking ownership, clinical reasoning, curiosity, dependability, and high ethical standards …

Chemical Disaster Preparedness For Hospitals And Emergency Departments, Gregory K. Wanner, Do, Sukhi Atti, Md, Edward Jasper, Md

Department of Emergency Medicine Faculty Papers

Preparing to evaluate and treat victims of a chemical exposure incident is one aspect of hospital disaster preparedness. Past chemical disasters, including terrorist attacks and industrial or transit accidents, have highlighted the need for hospital planning, preparation, and training. Emergency department and hospital staff members must be familiar with their facility-specific protocols and be trained for their individual roles during these incidents. This article provides a brief review of the requirements and guidelines related to chemical disaster response from a healthcare perspective. Resources for training and the evaluation of chemically contaminated patients are discussed. Decontamination procedures, including pre-hospital and hospital-based …

Effect Of Verbal Directions On Grip Strength Evaluated Using The Handheld Dynamometer, Laura Walsh, Otd, Otr/L, Cht, Marie-Christine Potvin, Phd, Otr/L

Department of Occupational Therapy Faculty Papers

Background: Grip strength measurement using the handheld dynamometer is a key aspect of the evaluation of an upper extremity injury. The handheld dynamometer manufacturer has established research-based guidelines outlining body positioning during grip strength measurement. However, verbal direction guidelines, while provided, have not yet been shown to be most effective through research. This study seeks to determine whether the use of one of two types of verbal directions, with and without encouragements, resulted in greater grip strength as measured by the handheld dynamometer.

Method: The grip strength of healthy females (n = 60) was compared using two sets of prerecorded …

The Bulletin: Sidney Kimmel Medical College At Thomas Jefferson University, Volume 68, Issue 2, Fall 2019

The Bulletin (formerly the Jefferson Medical College Alumni Bulletin)

This issue includes:

• Dean's Column - The 195th Commencement of SKMC
• Perspectives
• On Campus
• A Message from Elizabeth A. Dale - Reimagine Jefferson
• Discovery - Know Thy Self
• Seeing the Holistic Picture - Integrative medicine is taking techniques from the past to change the future of healthcare - and Jefferson is leading the charge
• Faculty Profile - David Nash, MD, MBA
• Student Profile - Carrie Walsh
• Alumni Profile - Austin Chiang, MD, MPH, GI Fellow '18
• Class Notes
• Class Agent
• In Memoriam
• By the Numbers - The Class of 2023

Eyelid Chalazion Or Not?, Jerry A. Shields, Sara E. Lally, Tatyana Milman, Carol L. Shields

Wills Eye Hospital Papers

No abstract provided.

Vascular Perfusion In Persistent Pupillary Membrane Of The Iris., Michael Chang, David Ancona-Lezama, Carol L. Shields

Wills Eye Hospital Papers

No abstract provided.

Teaching An Old Pain Medicine Society New Tweets: Integrating Social Media Into Continuing Medical Education., Kellie M Jaremko, Eric S Schwenk, Amy C S Pearson, Jonathan Hagedorn, Ankeet D Udani, Gary Schwartz, Nabil M Elkassabany, Anne Snively, Edward R Mariano

Department of Anesthesiology Faculty Papers

No abstract.

Ethical Standards For Cardiothoracic Surgeons' Participation In Social Media., Thomas K. Varghese, John W. Entwistle, John E. Mayer, Susan D. Moffatt-Bruce, Robert M. Sade

Department of Surgery Faculty Papers

No abstract provided.

Aneurysm Formation, Growth, And Rupture: The Biology And Physics Of Cerebral Aneurysms., Pavlos Texakalidis, Ahmad Sweid, Nikolaos Mouchtouris, Eric C. Peterson, Chrissa Sioka, Leonardo Rangel-Castilla, John Reavey-Cantwell, Pascal Jabbour

Department of Neurosurgery Faculty Papers

Cerebral aneurysms (CAs) are characterized by a pathological wall structure with internal elastic lamina and media disruption, which leads to focal weakened pouches of the arterial wall. The prevalence of unruptured CAs has been estimated to be 2%-5% in the general population. During the past few decades, the pathophysiological mechanisms behind the formation, growth, and rupture of CAs have been the focus of numerous research studies. In the present review, we have summarized the inflammatory pathways, genetics, and risk factors for the formation, growth, and rupture of CAs. In addition, we have discussed the concepts of geometric indexes, flow patterns, …

Decreasing Time To Initiation Of Chemotherapy For Patients Electively Admitted To A Hematologic Malignancy Service., Jose N Galeas, Stuart Packer, Roy Browne, Susan Sakalian, Adam F. Binder

Department of Medical Oncology Faculty Papers

Background: Delays in initiating elective inpatient chemotherapy can decrease patient satisfaction and increase length of stay. At our institution, we observed that 86% of patients admitted for elective chemotherapy experienced a delay (greater than 6 hours) with a median time to chemotherapy of 18.9 hours. We developed a process improvement initiative to improve time to chemotherapy for elective chemotherapy admissions.

Methods: Our outcome measure was time from admission to chemotherapy administration in patients admitted for elective chemotherapy. Process measures were identified and monitored. We collected baseline data and utilized performance improvement tools to identify key drivers. We focused on these …

Applications In Molecular Ultrasound Imaging: Present And Future, Vishal Thumar, Md, Ji-Bin Liu, Md, John Eisenbrey, Phd

Department of Radiology Faculty Papers

Molecular ultrasound imaging or targeted contrast-enhanced ultrasound (CEUS) is a relatively new technique that has varied applications to augment both diagnostics and therapeutics. Ultrasound contrast agents are conjugated to ligands that bind with specific biomarkers in the areas of interest which can then be quantified using ultrasound technology. This technique has numerous clinical applications including studying pathophysiology of disease, improving diagnostic sensitivity and specificity, and improving localized drug delivery. This technology, most notably, has proven useful in numerous oncologic and cardiovascular applications. Given ultrasound’s advantages over other radiographic studies including its low cost, lack of ionizing radiation, portability, ability to …

Cmut/Cmos-Based Butterfly Iq – A Portable Personal Sonoscope, Joyce Liu, Ba, Jiajun Xu, Md, Flemming Forsberg, Phd, Faium, Ji-Bin Liu, Md

Department of Radiology Faculty Papers

With the development of bioengineering technologies, medical ultrasound systems have evolved and advanced over the years, including the transition of ultrasound machines from analog devices to digital systems and improvements in transducer assembly from piezoelectric ceramics to single crystals. In the past several years, the further miniaturization of ultrasound units has become possible with the advancement of computer chip manufacturing and production. Most recently, a new handheld ultrasound system has been developed by the startup company Butterfly Network, using an innovative CMUT/CMOS-based probe technique. This paper will review the history, technology and features of this new device, as well as …

Clinical Value Of Ultrasonography For Monitoring Angular Pregnancy In First-Trimester, Yanhua Zhen, Md, Xuefeng Lu, Md, Chenyu Wang, Md, Huixia Li, Md, Ji-Bin Liu, Md

Department of Radiology Faculty Papers

Objective: To explore the clinical value of ultrasonography for early detection and monitoring of angular pregnancy during the first-trimester.

Methods: For this retrospective study, we enrolled 23 patients with asymptomatic angular pregnancy who were diagnosed by ultrasound at early pregnancy and who underwent ultrasound follow-up to determine its clinical outcome. An ultrasound unit E8 (GE Healthcare, Milwaukee, WI) associated with a 5-9 MHz convex array transvaginal probe was used to image the gestational sac by both two-dimensional and three-dimensional modes. Transvaginal sonographic observation included the location, size, and shape of the gestational sac, as well as the relationship between the …

Artificial Intelligence In Ultrasound Imaging: Current Research And Applications, Shuo Wang, Bs, Ji-Bin Liu, Md, Ziyin Zhu, Md, John Eisenbrey, Phd

Department of Radiology Faculty Papers

Artificial intelligence (AI) is an area of computer science that emphasizes the creation of intelligent software or system based on big data information, machine learning and deep learning technologies. The rapid development of science and technology as well as internet communication has enabled AI and big data to gradually apply to many fields of health care. The modern imaging medicine is one of the first areas that AI can play an important role and applications. As cross-sectional imaging, ultrasound (US) is well suitable for AI technology to standardize imaging protocols and improve diagnostic accuracy. This article reviews current AI technology …

Inactivated Rabies Virus-Based Ebola Vaccine Preserved By Vaporization Is Heat-Stable And Immunogenic Against Ebola And Protects Against Rabies Challenge., Drishya Kurup, Christine R. Fisher, Todd G. Smith, Tiago Abreu-Mota, Yong Yang, Felix R. Jackson, Nadia Gallardo-Romero, Richard Franka, Victor Bronshtein, Matthias J. Schnell

Department of Microbiology and Immunology Faculty Papers

BACKGROUND: Ebola virus (EBOV) is a highly lethal member of the Filoviridae family associated with human hemorrhagic disease. Despite being a sporadic disease, it caused a large outbreak in 2014-2016 in West Africa and another outbreak recently in the Democratic Republic of Congo. Several vaccine candidates are currently in preclinical and clinical studies but none are stable without cold chain storage.

METHODS: We used preservation by vaporization (PBV), a novel processing technology to heat-stabilize FiloRab1 (inactivated rabies-based Ebola vaccine), a candidate Ebola vaccine, and stored the vials at temperatures ranging from 4°C to 50°C for 10 days to 12 months. …

Effect On Nurse And Patient Experience: Overnight Use Of Blue-Depleted Illumination, Lorenzo Albala, Timothy Bober, Graham Hale, Benjamin Warfield, Micaela Langille Collins, Zachary Merritt, Eric Steimetz, Shmuel Nadler, Yair Lev, John Hanifin

Department of Neurology Faculty Papers

Background

Typical hospital lighting is rich in blue-wavelength emission, which can create unwanted circadian disruption in patients when exposed at night. Despite a growing body of evidence regarding the effects of poor sleep on health outcomes, physiologically neutral technologies have not been widely implemented in the US healthcare system.

Objective

The authors sought to determine if rechargeable, proximity-sensing, blue-depleted lighting pods that provide wireless task lighting can make overnight hospital care more efficient for providers and less disruptive to patients.

Design

Non-randomised, controlled interventional trial in an intermediate-acuity unit at a large urban medical centre.

Methods

Night-time healthcare providers abstained …

High-Fat Diet Fuels Prostate Cancer Progression By Rewiring The Metabolome And Amplifying The Myc Program., David P. Labbé, Giorgia Zadra, Meng Yang, Jaime M Reyes, Charles Y Lin, Stefano Cacciatore, Ericka M. Ebot, Amanda L. Creech, Francesca Giunchi, Michelangelo Fiorentino, Habiba Elfandy, Sudeepa Syamala, Edward D. Karoly, Mohammed Alshalalfa, Nicholas Erho, Ashley Ross, Edward M. Schaeffer, Ewan A. Gibb, Mandeep Takhar, Robert B. Den, Jonathan Lehrer, R. Jeffrey Karnes, Stephen J. Freedland, Elai Davicioni, Daniel E. Spratt, Leigh Ellis, Jacob D. Jaffe, Anthony V. DʼAmico, Philip W. Kantoff, James E. Bradner, Lorelei A. Mucci, Jorge E. Chavarro, Massimo Loda, Myles Brown

Department of Radiation Oncology Faculty Papers

Systemic metabolic alterations associated with increased consumption of saturated fat and obesity are linked with increased risk of prostate cancer progression and mortality, but the molecular underpinnings of this association are poorly understood. Here, we demonstrate in a murine prostate cancer model, that high-fat diet (HFD) enhances the MYC transcriptional program through metabolic alterations that favour histone H4K20 hypomethylation at the promoter regions of MYC regulated genes, leading to increased cellular proliferation and tumour burden. Saturated fat intake (SFI) is also associated with an enhanced MYC transcriptional signature in prostate cancer patients. The SFI-induced MYC signature independently predicts prostate cancer …