Digital Commons Network^™

Large-Scale Dna Demethylation Occurs In Proliferating Ovarian Granulosa Cells During Mouse Follicular Development, Tomoko Kawai, Joanne S Richards, Masayuki Shimada

Faculty, Staff and Students Publications

During ovarian follicular development, granulosa cells proliferate and progressively differentiate to support oocyte maturation and ovulation. To determine the underlying links between proliferation and differentiation in granulosa cells, we determined changes in 1) the expression of genes regulating DNA methylation and 2) DNA methylation patterns, histone acetylation levels and genomic DNA structure. In response to equine chorionic gonadotropin (eCG), granulosa cell proliferation increased, DNA methyltransferase (DNMT1) significantly decreased and Tet methylcytosine dioxygenase 2 (TET2) significantly increased in S-phase granulosa cells. Comprehensive MeDIP-seq analyses documented that eCG treatment decreased methylation of promoter regions in approximately 40% of the genes in granulosa …

Hematopoiesis Under Telomere Attrition At The Single-Cell Resolution, Natthakan Thongon, Feiyang Ma, Andrea Santoni, Matteo Marchesini, Elena Fiorini, Ashley Rose, Vera Adema, Irene Ganan-Gomez, Emma M Groarke, Fernanda Gutierrez-Rodrigues, Shuaitong Chen, Pamela Lockyer, Sarah Schneider, Carlos Bueso-Ramos, Guillermo Montalban-Bravo, Caleb A Class, Kelly A Soltysiak, Matteo Pellegrini, Ergun Sahin, Alison A Bertuch, Courtney D Dinardo, Guillermo Garcia-Manero, Neal S Young, Karen Dwyer, Simona Colla

Faculty, Staff and Students Publications

The molecular mechanisms that drive hematopoietic stem cell functional decline under conditions of telomere shortening are not completely understood. In light of recent advances in single-cell technologies, we sought to redefine the transcriptional and epigenetic landscape of mouse and human hematopoietic stem cells under telomere attrition, as induced by pathogenic germline variants in telomerase complex genes. Here, we show that telomere attrition maintains hematopoietic stem cells under persistent metabolic activation and differentiation towards the megakaryocytic lineage through the cell-intrinsic upregulation of the innate immune signaling response, which directly compromises hematopoietic stem cells' self-renewal capabilities and eventually leads to their exhaustion. …

High-Fructose, High-Fat Diet Alters Muscle Composition And Fuel Utilization In A Juvenile Iberian Pig Model Of Non-Alcoholic Fatty Liver Disease, Heather C Spooner, Stefani A Derrick, Magdalena Maj, Rodrigo Manjarín, Gabriella V Hernandez, Deepali S Tailor, Parisa S Bastani, Rob K Fanter, Marta L Fiorotto, Douglas G Burrin, Michael R La Frano, Angelos K Sikalidis, Jason M Blank

Children’s Nutrition Research Center Staff Publications

Non-alcoholic fatty liver disease (NAFLD) is a serious metabolic condition affecting millions of people worldwide. A “Western-style diet” has been shown to induce pediatric NAFLD with the potential disruption of skeletal muscle composition and metabolism. To determine the in vivo effect of a “Western-style diet” on pediatric skeletal muscle fiber type and fuel utilization, 28 juvenile Iberian pigs were fed either a control diet (CON) or a high-fructose, high-fat diet (HFF), with or without probiotic supplementation, for 10 weeks. The HFF diets increased the total triacylglycerol content of muscle tissue but decreased intramyocellular lipid (IMCL) content and the number of …

Perspectives Of Black And Hispanic Children Living In Under-Resourced Communities On Meal Preparation And Grocery Shopping Behaviors: Implications For Nutrition Education, Chishinga Callender, Denisse Velazquez, Meheret Adera, Jayna M Dave, Norma Olvera, Tzuan A Chen, Shana Alford, Debbe Thompson

Children’s Nutrition Research Center Staff Publications

Minority children living in under-resourced communities are at the greatest risk for obesity and poor diet quality. Child involvement in meal preparation may be a helpful strategy to improve diet quality. This paper explores minority children's perspectives regarding this. Eighteen children participated in a mixed methods study (online surveys, telephone interviews). Descriptive statistics were calculated for child demographic and psychosocial factors. Thematic analysis was used to code and analyze the interviews. Most children reported having cooking experience (83%) and cooking with family (94%) and exhibited high cooking self-efficacy (21.8 ± 2.9) and positive cooking attitudes (25.7 ± 4.4). Children reported …

Construct Validity For The Self-Reported Competency And Sub-Construct Associated Characteristics Of Romanian Physicians In Autism Spectrum Disorder, Mohammad H Rahbar, Iuliana Dobrescu, Shezeen Gillani, Manouchehr Hessabi, Sori Kim, Mihaela Stancu, Florina Rad

Faculty, Staff and Student Publications

BACKGROUND: Lack of physicians' knowledge regarding mental health, including Autism Spectrum Disorder (ASD) could have adverse effects on affected individuals' health and quality of life. The purpose of this study was to provide construct validity for a modified questionnaire in order to determine the self-reported competency for underlying sub-constructs in ASD, make inferences on perceived competence in ASD based on a sample of Romanian physicians, and identify physicians' characteristics associated with these sub-domains of competency.

METHODS: For this survey, we modified a questionnaire that was used in Pakistan and Turkey, and administered it to a sample of 383 practicing physicians …

Ms 224 Guide To Randall Wright Papers On Kazakhstan Health Care (1999-2000), Randall Wright

Manuscript Finding Aids

The Randall Wright papers on Kazakhstan health care are related to a Baylor College of Medicine and United Methodist Committee on Relief (UMCOR) conducted health care initiatives among populations in Kazakhstan and other regions the former Soviet Union. The initial assessment was related to the affects of above ground nuclear testing of weapons on regional populations. Randall Wright is a former administrator with Methodist Hospital and UMCOR. These papers are from materials he preserved from his work in the area in the 1990s. The collection includes photos, PowerPoint slides printed on paper, regional maps, correspondence, and memos. See more at …

A Community-Based Management Of Covid-19 In A Mobile Container Unit, Elena Petrova, Timothy Farinholt, Tejas P Joshi, Hannah Moreno, Mayar Al Mohajer, Shital M Patel, Joseph Petrosino, Sharmila Anandasabapathy

Faculty, Staff and Students Publications

Vaccine uptake is a multifactor measure of successful immunization outcomes that includes access to healthcare and vaccine hesitancy for both healthcare workers and communities. The present coronavirus disease (COVID-19) pandemic has highlighted the need for novel strategies to expand vaccine coverage in underserved regions. Mobile clinics hold the promise of ameliorating such inequities, although there is a paucity of studies that validate environmental infection in such facilities. Here, we describe community-based management of COVID-19 through a Smart Pod mobile clinic deployed in an underserved community area in the United States (Aldine, Harris County, TX, USA). In particular, we validate infection …

Evaluation Of Bone Formation On Orthopedic Implant Surfaces Using An Ex-Vivo Bone Bioreactor System, Rupak Dua, Hugh Jones, Philip C Noble

Faculty, Staff and Student Publications

Recent advances in materials and manufacturing processes have allowed the fabrication of intricate implant surfaces to facilitate bony attachment. However, refinement and evaluation of these new design strategies are hindered by the cost and complications of animal studies, particularly during early iterations in the development process. To address this problem, we have previously constructed and validated an ex-vivo bone bioreactor culture system that can maintain the viability of bone samples for an extended period ex-vivo. In this study, we investigated the mineralization of a titanium wire mesh scaffold under both static and dynamic culturing using our ex vivo bioreactor system. …

Nrf1 Association With Auts2-Polycomb Mediates Specific Gene Activation In The Brain, Sanxiong Liu, Kimberly A Aldinger, Chi Vicky Cheng, Takae Kiyama, Mitali Dave, Hanna K Mcnamara, Wukui Zhao, James M Stafford, Nicolas Descostes, Pedro Lee, Stefano G Caraffi, Ivan Ivanovski, Edoardo Errichiello, Christiane Zweier, Orsetta Zuffardi, Michael Schneider, Antigone S Papavasiliou, M Scott Perry, Jennifer Humberson, Megan T Cho, Astrid Weber, Andrew Swale, Tudor C Badea, Chai-An Mao, Livia Garavelli, William B Dobyns, Danny Reinberg

Faculty, Staff and Student Publications

The heterogeneous family of complexes comprising Polycomb repressive complex 1 (PRC1) is instrumental for establishing facultative heterochromatin that is repressive to transcription. However, two PRC1 species, ncPRC1.3 and ncPRC1.5, are known to comprise novel components, AUTS2, P300, and CK2, that convert this repressive function to that of transcription activation. Here, we report that individuals harboring mutations in the HX repeat domain of AUTS2 exhibit defects in AUTS2 and P300 interaction as well as a developmental disorder reflective of Rubinstein-Taybi syndrome, which is mainly associated with a heterozygous pathogenic variant in CREBBP/EP300. Moreover, the absence of AUTS2 or mutation in its …

Evaluating The Efficacy Of Automated Smoking Treatment For People With Hiv: Protocol For A Randomized Controlled Trial, Damon J Vidrine, Thanh C Bui, Michael S Businelle, Ya-Chen Tina Shih, Steven K Sutton, Lokesh Shahani, Diana Stewart Hoover, Kristina Bowles, Jennifer I Vidrine

Faculty, Staff and Student Publications

BACKGROUND: Smoking prevalence rates among people with HIV are nearly 3 times higher than those in the general population. Nevertheless, few smoking cessation trials targeting smokers with HIV have been reported in the literature. Efforts to develop and evaluate sustainable, low-cost, and evidence-based cessation interventions for people with HIV are needed. Given the widespread proliferation of mobile phones, the potential of using mobile health apps to improve the reach and efficacy of cessation interventions is promising, but evidence of efficacy is lacking, particularly among people with HIV.

OBJECTIVE: This study will consist of a 2-group randomized controlled trial to evaluate …

Gαi/O-Coupled Htr2c In The Paraventricular Nucleus Of The Hypothalamus Antagonizes The Anorectic Effect Of Serotonin Agents, Eun-Seon Yoo, Li Li, Lin Jia, Caleb C Lord, Charlotte E Lee, Shari G Birnbaum, Claudia R Vianna, Eric D Berglund, Kathryn A Cunningham, Yong Xu, Jong-Woo Sohn, Chen Liu

Children’s Nutrition Research Center Staff Publications

The anorexigenic effect of serotonergic compounds has largely been attributed to activation of serotonin 2C receptors (Htr2cs). Using mouse genetic models in which Htr2c can be selectively deleted or restored (in Htr2c-null mice), we investigate the role of Htr2c in forebrain Sim1 neurons. Unexpectedly, we find that Htr2c acts in these neurons to promote food intake and counteract the anorectic effect of serotonergic appetite suppressants. Furthermore, Htr2c marks a subset of Sim1 neurons in the paraventricular nucleus of the hypothalamus (PVH). Chemogenetic activation of these neurons in adult mice suppresses hunger, whereas their silencing promotes feeding. In …

Posttranslational Modifications In Pd-L1 Turnover And Function: From Cradle To Grave, Xinfang Yu, Wei Li, Ken H Young, Yong Li

Faculty, Staff and Students Publications

Programmed death-ligand 1 (PD-L1) is one of the most classic immune checkpoint molecules. Cancer cells express PD-L1 to inhibit the activity of effector T cells' cytotoxicity through programmed death 1 (PD-1) engagement in exposure to inflammatory cytokines. PD-L1 expression levels on cancer cells might affect the clinical response to anti-PD-1/PD-L1 therapies. Hence, understanding molecular mechanisms for regulating PD-L1 expression is essential for improving the clinical response rate and efficacy of PD-1/PD-L1 blockade. Posttranslational modifications (PTMs), including phosphorylation, glycosylation, ubiquitination, and acetylation, regulate PD-L1 stability, cellular translocation, and interaction with its receptor. A coordinated positive and negative regulation via PTMs is …

Can The Hunger Vital Sign™ Act As A Prescreen For Other Social Needs?, Richard Sheward, Charlotte Bruce, Deborah A. Frank, Sharon Coleman, Stephanie Ettinger De Cuba, Blair Robinson, Diana B. Cutts

Journal of Applied Research on Children: Informing Policy for Children at Risk

Background: Addressing health-related social needs is essential for improving health and reducing longstanding disparities. However, barriers to screening – including clinician and patient time burden of screening for multiple social needs – limit identification. To address this concern and promote the uptake of screening by clinicians, it is important that screening tools effectively and efficiently identify social needs’ presence and absence among patients.

Objective: This study evaluated whether a validated and widely implemented 2-question food insecurity screening tool, the Hunger Vital Sign™ (HVS™), has adequate negative predictive value to serve as a pre-screen for other social needs.

Methods: In 2007-2015, …

Strong Constraints From Cosine-100 On The Dama Dark Matter Results Using The Same Sodium Iodide Target, Govinda Adhikari, Estella B De Souza, Nelson Carlin, Jae Jin Choi, Seonho Choi, Mitra Djamal, Anthony C Ezeribe, Luis E França, Chang Hyon Ha, In Sik Hahn, Eunju Jeon, Jay Hyun Jo, Han Wool Joo, Woon Gu Kang, Matthew Kauer, Hyounggyu Kim, Hongjoo Kim, Kyungwon Kim, Sunghyun Kim, Sun Kee Kim, Won Kyung Kim, Yeongduk Kim, Yong-Hamb Kim, Young Ju Ko, Eun Kyung Lee, Hyunseok Lee, Hyun Su Lee, Hye Young Lee, In Soo Lee, Jaison Lee, Jooyoung Lee, Moo Hyun Lee, Seo Hyun Lee, Seung Mok Lee, Douglas Leonard, Bruno B Manzato, Reina H Maruyama, Robert J Neal, Stephen L Olsen, Byung Ju Park, Hyang Kyu Park, Hyeonseo Park, Kangsoon Park, Ricardo L C Pitta, Hafizh Prihtiadi, Sejin Ra, Carsten Rott, Keon Ah Shin, Andrew Scarff, Neil J C Spooner, William G Thompson, Liang Yang, Gyun Ho Yu

Faculty and Staff Publications

We present new constraints on dark matter interactions using 1.7 years of COSINE-100 data. The COSINE-100 experiment, consisting of 106 kg of tallium-doped sodium iodide [NaI(Tl)] target material, is aimed to test DAMA’s claim of dark matter observation using the same NaI(Tl) detectors. Improved event selection requirements, a more precise understanding of the detector background, and the use of a larger dataset considerably enhance the COSINE-100 sensitivity for dark matter detection. No signal consistent with the dark matter interaction is identified and rules out model-dependent dark matter interpretations of the DAMA signals in the specific context of standard halo model …

Mapk6-Akt Signaling Promotes Tumor Growth And Resistance To Mtor Kinase Blockade, Qinbo Cai, Wolong Zhou, Wei Wang, Bingning Dong, Dong Han, Tao Shen, Chad J Creighton, David D Moore, Feng Yang

Faculty, Staff and Students Publications

Mitogen-activated protein kinase 6 (MAPK6) is an atypical MAPK. Its function in regulating cancer growth remains elusive. Here, we reported that MAPK6 directly activated AKT and induced oncogenic outcomes. MAPK6 interacted with AKT through its C34 region and the C-terminal tail and phosphorylated AKT at S473 independent of mTORC2, the major S473 kinase. mTOR kinase inhibitors have not made notable progress in the clinic. Our identified MAPK6-AKT axis may provide a major resistance pathway. Besides repressing growth, inhibiting MAPK6 sensitized cancer cells to mTOR kinase inhibitors. MAPK6 overexpression is associated with decreased overall survival and the survival of patients with …

Ms 143 Guide To Vernie Stembridge Md Papers (1945-2000), Vernie A. Stembridge (1924-2000)

Manuscript Finding Aids

The Vernie A. Stembridge, MD papers contains professional papers, military records, reprints, administrative records, correspondence, awards, photographs, realia, oversized materials, personal papers, artifacts, yearbooks, textbooks, monographs, printed materials, memorabilia, ceritifactes, artwork, and audiovisual materials. See more at MS 143.

Ms 087 Guide To Connie Brady Nursing School Papers (1960-1964), Connie Brady

Manuscript Finding Aids

Connie Brady was a nursing student at the Shannon West Texas Memorial Hospital School of Nursing in San Angelo, Tom Green County, Texas, in 1960-1964. This collection includes a 1964 diary Brown wrote for a nursing course, school-related correspondence and papers, school publications, and nursing publications. See more at MS 087.

Ms 095 Guide To Raymond C. Anderson, Md Papers (1947-1949), Raymond C. Anderson (1918-2008)

Manuscript Finding Aids

The Raymond C. Anderson, MD, PhD papers comprises photocopies of Anderson's journal and the photographs he took during his time at the Atomic Bomb Casualty Commission. It is believed that Anderson's family still retains the original copies of the diary and photographs. See more at MS 095.

Ms 213 Guide To Teresa Hayes, Md, Phd Papers (1994-2002), Teresa Hayes

Manuscript Finding Aids

The Teresa Hayes, MD, PhD papers contains photographs, artifacts, documents, speeches, and books related to Dr. Hayes’ work with Dr. Armin Weinberg radiation effects and events projects and the United Methodist Health Initiative. in Kazakhstan and Russia. The collection equals about .5 cubic foot (1 box) and includes 286 photographs. Find out more from MS 213.

Ms 052 Guide To George J. Ehni, Md Papers (1940-1983), George Ethni (1914-1986)

Manuscript Finding Aids

George J. Ehni, MD papers contains documents relating to Dr. Ehni’s life and career in neurology, including correspondence, manuscripts, research materials, and reprints. Some of these materials appear to be related to lectures or talks given by Ehni. See more at MS 052.

Ms 218 Guide To The Reginald Burbank, Md Papers (1820-1973), Reginald Burbank (1888-1972)

Manuscript Finding Aids

The Reginald Burbank, MD papers (MS218) is 2.5 cubic feet and consists of 5 documents boxes. Dr. Burbank’s papers include institutional publications, medical research, translations, books, pamphlets, correspondence, letters, newspaper clippings, typed manuscripts, typed manuscripts (drafts), and medical journals that document his research and career. See more at MS 218.

Ms 208 Guide To The Joyce E. Yost, Phd Papers (1983-1997), Joyce E. Yost

Manuscript Finding Aids

The Joyce E. Yost, PhD papers consist of 7 boxes and contains newspaper clippings, correspondence, printed material, speeches, magazines, newsletters, manuals, books, studies, reports, reprints, prophylactic material, financial documents, and meeting minutes and materials that document Yost’s doctoral dissertation research. Collection includes information related to the Montrose Clinic and AIDS Foundation Houston (AFH), and other AIDS organizations, activist groups and programs, See more at MS 208.

Ms 142 Guide To The William H. Ellett Atomic Bomb Casualty Commission Papers, (1945-1992), William H. Ellett

Manuscript Finding Aids

The William H. Ellett Atomic Bomb Casualty Commission papers includes a 1945 topographical map of Nagasaki used in planning the atomic attack and a 1946 topographical map of Hiroshima that was used by the international damage assessment group. The collection also includes an unpublished manuscript of ABCC history written by John Z. Bowers, a former ABCC staffer. Ellett also included his personal copies of the "Life Span Study" reports. The size of the collection is one cubic foot (2 boxes and two oversize maps). See more at MS 142.

Ms 109 Guide To Kanellos D. Charalampous, Md Papers (1968-1981), Kanellos D. Charalampous

Manuscript Finding Aids

Kanellos D. Charalampous, MD papers contains reel-to-reel audiotapes of lectures for a course in social and community psychiatry with presentations by Titus Harris, Jack Ewalt, John Spiegal, Harry Brickman, Samuel Braun, Betty Caldwell, Eli Bower, David Sanders, Phyllis Rolfe Silverman, and other pioneers in psychiatry. See more at MS 109.

Ms 093 Guide To Marvin A. Kastenbaum, Ph.D., Papers (1950-1997), Marvin A. Kastenbaum (1926-2019)

Manuscript Finding Aids

The Marvin A. Kastenbaum, PhD, papers, MS 93, 1950-1997, contains materials related to the Atomic Bomb Casualty Commission (ABCC), including audio-visual materials, photographs, artifacts, personal cards, clippings, and statistical analyses compilied by the ABCC. Kastenbaum worked for 17 months as a statistician with the ABCC. Kastenbaum's first contribution to the archive in March 1994 was a set of photographs of ABCC employees. See more at MS 093.

Ms 005 Guide To Valliant Clinton Baird, Md Papers (1946-1978), Valliant Clinton Baird (1904-1986)

Manuscript Finding Aids

The Valliant Clinton Baird, MD, papers is 1/2 cubic feet and consists of 1 document box. It contains biographical information, personal and professional correspondence, official documents, news clippings, that detail the personal life, professional activities, medical related records and papers associated with the experiences of Dr. Baird company physician Exon Mobil, and also his contributions to the Houston Texas Medical Center. The materials are in good condition. Ink applied by typewriters is clearly legible in the documents. The records pertain to the geographic areas of New Orleans Louisiana and Houston, Texas. See more at MS 005.

Ms 030 Guide To L. Rodney Rodgers, Md Papers (1940-1994), L. Rodney Rogers (1920-2012)

Manuscript Finding Aids

The L. Rodney Rodgers, MD papers consists of announcements, correspondence, articles, handbooks, ethics papers, Harris County Medical society meeting records, reprints and other printed material related to L. Rodney Rodgers career in internal medicine. See more at MS 030.

Ms 035 Guide To Wataru W. Sutow, Md Papers (1929-1996), Wataru W. Sutow (1912-1981)

Manuscript Finding Aids

Watauru W. Sutow, MD papers, primarily cover the professional life of Dr. Sutow. The collection contains correspondence and memorandum, committee minutes and reports, drafts, manuscripts, and published professional papers; journal article reprints, personal correspondence and memorabilia; and a collection of slides and audio cassette tapes. The collection is in good condition. The papers span the years 1929-1996 with the bulk of material ranging from 1948 to 1981. The collection consists of 43 cubic feet (86 boxes, including 1 oversize box). See more at MS 035.

Ms 077 Guide To Griff Ross, Md Papers (1984), Griff T. Goss (1920-1985)

Manuscript Finding Aids

The Griff Ross, MD papers contains two bound volumes of the 1984 oral interview transcript with Dr. Ross. The first volume contains Dr. Ross's interview; the second contains interviews with four of his colleagues. In the interview, Dr. Ross discusses both his personal and professional life.The collection consists on one box equaling 0.25 cubic feet. Materials are in good condition. See more at MS 077.

Ms 090 Guide To Ivan F. Duff, Md Papers (1967-1993), Ivan F. Duff Md

Manuscript Finding Aids

The Ivan Frances Duff, MD, papers, MS 90, consists of Dr. Duff's work in the field of rheumatology and his professional work in China and in Japan. The collection covers the years 1966-1993. See more at MS 090.