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New Data On Distribution, Ecology, And Taxonomy Of Turkish Nitidulidae (Coleoptera), Saki̇ne Serap Avgin, Gloria Antonini, Andrzej Lason, Nicklas Jansson, Tuba Öncül Abacigi̇l, Saki̇n Vural Varli, Alessio De Biase, Paolo Audisio
New Data On Distribution, Ecology, And Taxonomy Of Turkish Nitidulidae (Coleoptera), Saki̇ne Serap Avgin, Gloria Antonini, Andrzej Lason, Nicklas Jansson, Tuba Öncül Abacigi̇l, Saki̇n Vural Varli, Alessio De Biase, Paolo Audisio
Turkish Journal of Zoology
This paper updates our present knowledge on the geographical distribution and host plant relationships of Turkish species of the sap- and pollen-beetle family Nitidulidae. Pria zenobia Jelínek, 1997 from eastern Mediterranean areas is synonymized with Pria angustula Cooper, 1982, from South Africa (syn. nov.). The unknown ovipositor of Xerogethes osellai Audisio & Jelínek, 2000, a species endemic to central Turkey, is described, and the first information on its biology is reported. Glischrochilus quadripunctatus (Linnaeus, 1758) (Tunceli Province) and Pityophagus quercus Reitter, 1877 (Balikesir Province) are first recorded for the Turkish fauna. The distributions of Glischrochilus hortensis (Geoffroy in Fourcroy, 1785), …
Sensitivity Of Soil Evaporation And Reference Evapotranspirationto Climatic Variables In South Korea, Mehmet Aydin, Yeong-Sang Jung, Jae E. Yang, Su-Jung Kim, Kyung-Dae Kim
Sensitivity Of Soil Evaporation And Reference Evapotranspirationto Climatic Variables In South Korea, Mehmet Aydin, Yeong-Sang Jung, Jae E. Yang, Su-Jung Kim, Kyung-Dae Kim
Turkish Journal of Agriculture and Forestry
The quantification of evapotranspiration and soil evaporation is crucial for agricultural water management. The FAO-56 Penman-Monteith and E-DiGOR models were used to compute reference evapotranspiration (Eto) and bare soil evaporation, respectively, at 17 meteorological stations of South Korea, from 1980 to 2009. The same soil parameters were assumed for all stations in order to compare actual soil evaporation (Ea) rates jointly dominated by atmospheric evaporative demand and soil water availability, as well as the size of rainfall events. The sensitivity of Penman-Monteith type equations to the major climatic variables was determined based on 1-year dataset. The long-term mean annual precipitation …
Effect Of Strainer Type, Spray Pressure, And Orifice Size On The Discharge Coefficient Of Standard Flat-Fan Nozzles, Bahadir Sayinci
Effect Of Strainer Type, Spray Pressure, And Orifice Size On The Discharge Coefficient Of Standard Flat-Fan Nozzles, Bahadir Sayinci
Turkish Journal of Agriculture and Forestry
The aim of this study was to determine the effect of nozzle strainer type (cylindrical, ball check, slotted, and cup screen type), spray pressure, and nozzle orifice size on the discharge coefficient (Cd) of standard flat-fan nozzles of different nominal sizes (from 02 to 06) and 110° spray angle. The flow rates measured for each nozzle orifice size and strainer type combinations were determined at five different spray pressures (2.0, 3.5, 5.0, 6.5, and 8.0 bars). In the study, size, shape, and area measurements concerned with nozzle geometry were performed and the differences between nozzles of different nominal sizes were …
Transcriptome Analysis Of Banana (Musa Balbisiana) Basedon Next-Generation Sequencing Technology, Suthanthiram Backiyarani, Subbaraya Uma, Marimuthu Somasundram Saraswathi, Asoor Santhanam Saravanakumar, Arumugam Chandrasekar
Transcriptome Analysis Of Banana (Musa Balbisiana) Basedon Next-Generation Sequencing Technology, Suthanthiram Backiyarani, Subbaraya Uma, Marimuthu Somasundram Saraswathi, Asoor Santhanam Saravanakumar, Arumugam Chandrasekar
Turkish Journal of Agriculture and Forestry
Banana (Musa spp.) is an important tropical fruit with high commercial potential. Musa balbisiana (B genome) is a progenitor of one of the most cultivated banana species and exhibits unique traits, including resistance or tolerance to many biotic and abiotic stresses. RNA sequencing of the Musa B genome would provide a vast array of transcriptomic information that could lead to the development of trait-specific markers and the discovery of new genes and regulatory sequences involved in resistance mechanisms. Thus, transcriptome sequencing was performed in Musa B genome accession Attikol using the Ion Torrent platform. This led to the generation of …
Incidence Of Microbial Infections Revealed By Assessing Nodulation Infield-Collected Insects From Adana Province, Hasan Tunaz, Mehmet Kubi̇lay Er, Ali̇ Arda Işikber
Incidence Of Microbial Infections Revealed By Assessing Nodulation Infield-Collected Insects From Adana Province, Hasan Tunaz, Mehmet Kubi̇lay Er, Ali̇ Arda Işikber
Turkish Journal of Agriculture and Forestry
Results of a field study designed to assess the extent of natural microbial infections in insects collected from agrarian fields surrounding Adana, Turkey, are reported. We identified and dissected specimens to assess the numbers of nodules. Formation of darkened melanotic nodules is the predominant cellular immune reaction to microbial and parasitic infection, and once formed, the nodules are permanently attached to internal surfaces. At least some nodules were found in 99% of 1200 examined specimens that were healthy in appearance. The number of nodules ranged approximately from 1 nodule/individual to >120 nodules/individual. We inferred that insects are regularly challenged by …
Short-Term Hydrological Responses To Silvicultural Treatments Withina Stream Buffer Zone: A Case Study, Ferhat Kara, Latif Kalin, Edward Francis Loewenstein
Short-Term Hydrological Responses To Silvicultural Treatments Withina Stream Buffer Zone: A Case Study, Ferhat Kara, Latif Kalin, Edward Francis Loewenstein
Turkish Journal of Agriculture and Forestry
The thinning of stream buffer zones (SBZs) is gaining popularity as a silvicultural management practice in order to reduce the risk of wildfire and insect hazard, provide economic return, and improve the effectiveness of SBZs. In this study, streamflow over a 1-year period was monitored at 2 small paired watersheds (treated vs. reference). The short-term impacts of a partial cutting within a SBZ as well as the relative effects of pastoral, forested, and clearcut areas on changes in downstream hydrology were examined. Upstream pastoral areas had a higher water yield than downstream forested sections during the no-harvest (calibration) period of …
Barley Molybdenum Cofactor Sulfurase (Mcsu): Sequencing, Modeling, And Its Comparison To Other Higher Plants, Ertuğrul Fi̇li̇z, Assaf Distelfeld, Tzion Fahima, Özge Karakaş Meti̇n, Eviatar Nevo, Song Weining, Ahu Altinkut Uncuoğlu
Barley Molybdenum Cofactor Sulfurase (Mcsu): Sequencing, Modeling, And Its Comparison To Other Higher Plants, Ertuğrul Fi̇li̇z, Assaf Distelfeld, Tzion Fahima, Özge Karakaş Meti̇n, Eviatar Nevo, Song Weining, Ahu Altinkut Uncuoğlu
Turkish Journal of Agriculture and Forestry
Molybdenum cofactor sulfurases (MCSUs) are important enzymes for plant development and response to environmental queues, including processes such as nitrogen metabolism and regulation of the abscisic acid levels in plant tissues. We cloned and sequenced MCSU gene from barley and performed in silico comparison with rice, tomato, and Arabidopsis. Physico-chemical properties and subcellular predictions were found to be similar in different plant species. All MCSUs had three critical domains: aminotransferase class-V (Pfam: PF00266), MOSC N-terminal beta barrel (Pfam: PF03476), and MOSC (Pfam: PF03473). Secondary structure analysis revealed that random coils were the most abundant, followed by α-helices and extended strands. …
Electrochemical Monitoring Of The Interaction Between Mitomycin C And Dna At Chitosan--Carbon Nanotube Composite Modified Electrodes, Pembe Ece Canavar, Ece Ekşi̇n, Kadri̇ye Arzum Erdem Gürsan
Electrochemical Monitoring Of The Interaction Between Mitomycin C And Dna At Chitosan--Carbon Nanotube Composite Modified Electrodes, Pembe Ece Canavar, Ece Ekşi̇n, Kadri̇ye Arzum Erdem Gürsan
Turkish Journal of Chemistry
Single-walled carbon nanotube (CNT) and chitosan composite (chitosan*CNT) based sensors were developed as DNA biosensors, and then they were applied for electrochemical investigation of the interaction between the anticancer drug mitomycin C (MC) and DNA. The oxidation signals of MC and guanine were monitored before and after the interaction process by differential pulse voltammetry (DPV). The DPV results were in good agreement with those of electrochemical impedance spectroscopy (EIS). Analytical parameters such as DNA concentration, MC concentration, and MC interaction time with DNA were optimized. The detection limits were 6.85 \mu g/mL for DNA and 11.01 \mu g/mL for MC.
Amperometric Biosensing Of Ethanol Based On Integration Of Alcohol Dehydrogenase With A Pt/Ppy--Pvs/Mb Electrode, Özlem Çolak, Fatma Arslan
Amperometric Biosensing Of Ethanol Based On Integration Of Alcohol Dehydrogenase With A Pt/Ppy--Pvs/Mb Electrode, Özlem Çolak, Fatma Arslan
Turkish Journal of Chemistry
A novel amperometric ethanol biosensor with alcohol dehydrogenase (ADH) and nicotinamide adenine dinucleotide (NAD^+) on polypyrrole--polyvinylsulfonate (PPy-PVS) film was accomplished. Meldola's blue (MB) was used as a mediator; it can facilitate the electron transfer that involves a chemical interaction between the electrode surface and NADH. Determination of ethanol was carried out by the oxidation of MB at --0.072 V vs. Ag/AgCl. The effects of pH and temperature were investigated and optimum parameters were found to be 7.0 and 40 °C, respectively. There are 2 linear parts at 1.0 \mu M--10.0 \mu M (R^2 = 0.993) and 0.01 mM--0.1 mM (R^2 …
Synthesis And Biological Evaluation Of New Pyridines Containing Imidazole Moiety As Antimicrobial And Anticancer Agents, Ikhlass Abbas, Sobhi Gomha, Mahmoud Elaasser, Mohammed Bauomi
Synthesis And Biological Evaluation Of New Pyridines Containing Imidazole Moiety As Antimicrobial And Anticancer Agents, Ikhlass Abbas, Sobhi Gomha, Mahmoud Elaasser, Mohammed Bauomi
Turkish Journal of Chemistry
The synthesis of a novel series of pyridine and bipyridine derivatives is described via one-pot multicomponent reaction of 5-acetylimidazole, malonitrile (or ethylcyanoacetate or diethylmalonate), substituted benzaldehyde (or terephthaldehyde), and ammonium acetate in good yields. The structures of all the new compounds were elucidated on the basis of elemental analysis and spectral data. The antimicrobial activities of the synthesized compounds were screened and the results showed that most of such compounds exhibit considerable activities. Furthermore, some of the newly synthesized compounds were screened for their anticancer activity against human breast cell line (MCF-7) and liver carcinoma cell line (HEPG2) in comparison …
Analysis Of Interactions Of Nitric Oxide And Polyamine Under Cadmium Stress In Wheat, Fatma Mutlu, Füsun Yürekli̇
Analysis Of Interactions Of Nitric Oxide And Polyamine Under Cadmium Stress In Wheat, Fatma Mutlu, Füsun Yürekli̇
Turkish Journal of Botany
Wheat cultivars chosen for their cadmium (Cd) tolerance (Sönmez-2001) and sensitivity (Quality) were grown in Hoagland solution for 20 days and then they were treated with 100 μM 2-(4-carboxyphenyl)-4,4,5,5-tetramethylylimidazoline-1-oxyl (cPTIO), a nitric oxide (NO) scavenger, or with 100 μM sodium nitroprusside (SNP), a NO donor, with and without 9 mM Cd. NO, free polyamines, and Cd levels were analysed by using leaf and root samples taken 24 and 72 h after treatment. There was a significant increase in NO level in the Cd+SNP treatment in cv. Quality cultivars at 24 and 72 h. The NO level recorded in cv. Quality …
Clonal Propagation And Synthetic Seed Production From Nodal Segments Ofcape Gooseberry (Physalis Peruviana L.), A Tropical Fruit Plant, Bahti̇yar Buhara Yücesan, Ali̇yu Mohammed, Merve Arslan, Ekrem Gürel
Clonal Propagation And Synthetic Seed Production From Nodal Segments Ofcape Gooseberry (Physalis Peruviana L.), A Tropical Fruit Plant, Bahti̇yar Buhara Yücesan, Ali̇yu Mohammed, Merve Arslan, Ekrem Gürel
Turkish Journal of Agriculture and Forestry
Physalis peruviana L. contains polyphenols and carotenoids with antiinflammatory and antioxidant activities used against diabetes. To establish an efficient regeneration system using nodal segments excised from 4-week-old germinated seedlings, direct plant regeneration, without additional rooting stage, was achieved on LS medium containing 0.5 mg/L 6-benzylaminopurine (BAP), kinetin (KIN), thidiazuron (TDZ), or gibberellic acid (GA3), alone or in combination with 0.25 mg/L indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA), after 2 weeks of incubation. The highest mean numbers of shoots and well-developed roots were obtained on LS medium containing solely 0.5 mg/L TDZ, producing 5.3 shoots and 3.3 roots per explant …
Synthesis, Characterization, Biological Studies, And Molecular Modeling Of Mixed Ligand Bivalent Metal Complexes Of Schiff Bases Based On $N$-Aminopyrimidine-2-One/2-Thione, Hati̇ce Gamze Soğukömeroğullari, Tuğba Taşkin Tok, Feyza Yilmaz, İsmet Berber, Mehmet Sönmez
Synthesis, Characterization, Biological Studies, And Molecular Modeling Of Mixed Ligand Bivalent Metal Complexes Of Schiff Bases Based On $N$-Aminopyrimidine-2-One/2-Thione, Hati̇ce Gamze Soğukömeroğullari, Tuğba Taşkin Tok, Feyza Yilmaz, İsmet Berber, Mehmet Sönmez
Turkish Journal of Chemistry
New mixed Schiff bases, Cu(II), Co(II), Ni(II), and Mn(II) complexes, were synthesized derived from 5-chloro-2-hydroxyacethophenone and 1-amino-5-benzoyl-4-phenyl-1H-pyrimidine-2-one/thione. These complexes were characterized by elemental analysis, magnetic measurements, molar conductivity, IR, electronic, NMR, and mass spectral studies. All the complexes showed nonelectrolytic behavior. Moreover, the newly synthesized mixed ligand complexes were evaluated for their in vitro antimicrobial efficiency against bacteria and yeast. The compound named Co(L$_{1}$L) had good antifungal activity against Candida species, but no profound antibacterial effect against bacterial strains. In addition, the ground state geometries of the complexes were optimized using a semi-empirical method at PM6 level, which is a …
Simultaneous Analysis Of Losartan, Its Active Metabolite, And Hydrochlorothiazide In Human Plasma By A Uplc-Ms/Ms Method, Priyanka A. Shah, Primal Sharma, Jaivik V. Shah, Mallika Sanyal, Pranav S. Shrivastav
Simultaneous Analysis Of Losartan, Its Active Metabolite, And Hydrochlorothiazide In Human Plasma By A Uplc-Ms/Ms Method, Priyanka A. Shah, Primal Sharma, Jaivik V. Shah, Mallika Sanyal, Pranav S. Shrivastav
Turkish Journal of Chemistry
A selective and sensitive ultra performance liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of losartan (LOS), EXP-3174, which is an active metabolite LOS carboxylic acid, and hydrochlorothiazide (HCTZ) in human plasma. Solid-phase extraction was carried out on Oasis HLB cartridges with 100 $\mu $L of plasma to give an extraction recovery in the range of 88.5%-102.5% for the three analytes. Chromatography on a BEH C18 column afforded baseline separation of all the analytes within 2.4 min using 1.0% formic acid in water and acetonitrile (15:85, v/v) as the mobile phase. Quantitation was performed with multiple reaction …
Synthesis Of Novel 1,2,4-Triazoles And Triazolo-Thiadiazines As Anticancer Agents, Thoraya Abd El-Reheem Farghaly, Magda Ahmad Abdallah, Huda Kamel Mmahmoud
Synthesis Of Novel 1,2,4-Triazoles And Triazolo-Thiadiazines As Anticancer Agents, Thoraya Abd El-Reheem Farghaly, Magda Ahmad Abdallah, Huda Kamel Mmahmoud
Turkish Journal of Chemistry
A new series of 7-arylazo-5$H$-3-(trifluoromethyl)-6-methyl-1,2,4-triazolo-[3,4-$b$]-1,3,4-thiadiazines was prepared by reaction of 4-amino-3-trifluoromethyl-5-mercapto-1,2,4-triazoles with $N$-aryl-2-oxo-propane hydrazonoyl chloride in dioxane under reflux in the presence of triethylamine. Furthermore, Schiff bases of 4-amino-5-mercapto-1,2,4-triazole derivatives were reacted with a variety of hydrazonoyl chlorides and gave the respective hydrazonothioates. In addition, the novel \textit{bis}-(1,2,4-triazole-3-thione) was reacted with the appropriate hydrazonoyl chloride in dioxane under reflux in the presence of triethylamine to give the corresponding \textit{bis}-(1,2,4-triazolethiohydrazonoate). The structures of the new compounds were established based on elemental and spectral data. The mechanism of the studied reaction was also discussed. Moreover, some of the new products were screened …
Phytoremediation Potential Of Landoltia Punctata On Petroleum Hydrocarbons, Özlem Erteki̇n, Taylan Kösesakal, Vesi̇le Selma Ünlü, Sönmez Dağli, Volkan Peli̇tli̇, Hüma Uzyol, Yasemi̇n Tuna, Oktay Külen, Bayram Yüksel, Selma Onarici, Bi̇rsen Cevher Keski̇n, Abdülrezzak Memon
Phytoremediation Potential Of Landoltia Punctata On Petroleum Hydrocarbons, Özlem Erteki̇n, Taylan Kösesakal, Vesi̇le Selma Ünlü, Sönmez Dağli, Volkan Peli̇tli̇, Hüma Uzyol, Yasemi̇n Tuna, Oktay Külen, Bayram Yüksel, Selma Onarici, Bi̇rsen Cevher Keski̇n, Abdülrezzak Memon
Turkish Journal of Botany
In this study, the potential of Landoltia punctata for petroleum phytoremediation was evaluated. L. punctata was treated with different concentrations of Batman crude oil (BCO). The plants were grown in nutrient solutions containing 0.5%, 1%, 2%, and 3% BCO by volume in a growth chamber for 7 days. The fresh weight of the plants in the presence of BCO at a concentration of 0.5% v/v increased by 60% relative to the initial fresh weight. Furthermore, in comparison to the control, plant growth was retarded by 72% and 91% at concentrations of 2% and 3%, respectively. When plants were grown in …
Synecological Structure Of The Lichen Synusiae Within Forest Natural Reserves From The Moldavian Plateau (Romania), Ioana Vicol
Synecological Structure Of The Lichen Synusiae Within Forest Natural Reserves From The Moldavian Plateau (Romania), Ioana Vicol
Turkish Journal of Botany
This study describes and assesses the influence of the particular features of microhabitats on the abundances of lichen species. The research was performed in 4 natural reserves: Seaca-Movileni, Badeana, Harboanca-Brahaşoaia, and Balteni (all within Vaslui county). The distribution of lichen species abundances was investigated in 20 cm × 20 cm sampling units at a height of 1 m above the ground. In the studied area 27 lichen species were sampled in 81 sampling units. Cluster analysis indicated both similarities and dissimilarities between the natural reserves investigated. Kruskal-Wallis tests pointed out significant differences regarding tree girths, depth of rhytidome crevices, and …
In Vitro Antiproliferative/Cytotoxic Activity Of 2,3'-Biindole Against Various Cancer Cell Lines, Sali̇h Ökten, Ramazan Erenler, Tuğba Kul Köprülü, Şaban Teki̇n
In Vitro Antiproliferative/Cytotoxic Activity Of 2,3'-Biindole Against Various Cancer Cell Lines, Sali̇h Ökten, Ramazan Erenler, Tuğba Kul Köprülü, Şaban Teki̇n
Turkish Journal of Biology
2,3'-Biindole (2) was synthesized via bromination of indole (1) with molecular bromine and underwent simultaneous dimerization. Antiproliferative and cytotoxic activity of 2 was investigated in vitro on C6 (rat brain tumor), HeLa (human cervix carcinoma), and HT29 (human colon carcinoma) cells lines by using BrdU cell proliferation ELISA and lactate dehydrogenase (LDH) assays. In contrast to 5-fluorouracil (5-FU), 2,3'-biindole (2) significantly inhibited proliferation of HeLa and HT29 cell lines. According to LDH assay, the cytotoxicity of compound 2 was low on HT29 cell lines and high on HeLa and C6 cell lines. Moreover, 2 did not cause any DNA laddering …
Cytotoxic Effects Of Various Lactic Acid Bacteria On Caco-2 Cells, Sevda Er, Ayşe Tansu Koparal, Meri̇h Kivanç
Cytotoxic Effects Of Various Lactic Acid Bacteria On Caco-2 Cells, Sevda Er, Ayşe Tansu Koparal, Meri̇h Kivanç
Turkish Journal of Biology
Probiotics are live microbial food supplements that can be considered a functional food. They benefit the health of a host animal by maintaining their intestinal microbial balance. Most probiotic microorganisms are lactic acid bacteria (LAB) such as Lactobacillus spp., Bifidobacterium spp., and Enterococcus spp. LAB have been reported to possess certain anticancer properties. The vast majority of studies on their anticancer effects have dealt with colorectal cancers, although there have also been some studies on breast and bladder cancers. Colon cancer is the fourth most common cause of cancer-related mortality in the world. The aim of this study was to …
Identification And Pathogenicity Of Bacteria In The Mediterranean Corn Borer Sesamia Nonagrioides Lefebvre (Lepidoptera: Noctuidae), Ardahan Eski̇, Fi̇li̇z Özkan Çakici, Mustafa Güllü, Hacer Muratoğlu, Zi̇hni̇ Demi̇rbağ, İsmai̇l Demi̇r
Identification And Pathogenicity Of Bacteria In The Mediterranean Corn Borer Sesamia Nonagrioides Lefebvre (Lepidoptera: Noctuidae), Ardahan Eski̇, Fi̇li̇z Özkan Çakici, Mustafa Güllü, Hacer Muratoğlu, Zi̇hni̇ Demi̇rbağ, İsmai̇l Demi̇r
Turkish Journal of Biology
Sesamia nonagrioides (Lep.: Noctuidae) is one of the most serious pests of corn in Turkey and other Mediterranean countries. Although various cultural, chemical, and biological methods are used to control this pest, its damage still continues in all Mediterranean countries. In this study, to find an effective bacterium that can be used as a biocontrol agent against S. nonagrioides, we isolated 15 bacteria from S. nonagrioides larvae and evaluated the larvicidal potency of all isolates on the pest. According to their morphological, physiological, biochemical, and molecular properties, the isolates were identified as Achromobacter insolitus (Sn1), Morganella morganii (Sn2), Klebsiella pneumoniae …
Cloning And Expression Of Chitinase A, B, And C (Chia, Chib, Chic) Genes From Serratia Marcescens Originating From Helicoverpa Armigera And Determining Their Activities, Mehtap Danişmazoğlu, İsmai̇l Demi̇r, Kazim Sezen, Hacer Muratoğlu, Remzi̇ye Nalçacioğlu
Cloning And Expression Of Chitinase A, B, And C (Chia, Chib, Chic) Genes From Serratia Marcescens Originating From Helicoverpa Armigera And Determining Their Activities, Mehtap Danişmazoğlu, İsmai̇l Demi̇r, Kazim Sezen, Hacer Muratoğlu, Remzi̇ye Nalçacioğlu
Turkish Journal of Biology
Three genes encoding chitinase A (chiA), B (chiB), and C (chiC) were amplified from a bacterium that was isolated from a naturally dead Helicoverpa armigera larva and identified as Serratia marcescens based on 16S rRNA gene sequence analysis (KF823633 accession number). The open reading frames (ORFs) were identified as 1692, 1500, and 1443 base pairs for chiA, chiB, and chiC genes, respectively. These sequences were submitted to the GenBank with accession numbers KF823630 (chiA), KF823631 (chiB), and KF823632 (chiC). Comparison of the deduced amino acid sequences with those of other bacterial chitinases revealed that the 3 chitinases contain the catalytic …
Bone Marrow Stem Cells Adapt To Low-Magnitude Vibrations By Altering Their Cytoskeleton During Quiescence And Osteogenesis, Levent Demi̇ray, Engi̇n Özçi̇vi̇ci̇
Bone Marrow Stem Cells Adapt To Low-Magnitude Vibrations By Altering Their Cytoskeleton During Quiescence And Osteogenesis, Levent Demi̇ray, Engi̇n Özçi̇vi̇ci̇
Turkish Journal of Biology
Application of mechanical vibrations is anabolic to bone tissue, not only by guiding mature bone cells to increased formation, but also by increasing the osteogenic commitment of progenitor cells. However, the sensitivity and adaptive response of bone marrow stem cells to this loading regimen has not yet been identified. In this study, we subjected mouse bone marrow stem cell line D1-ORL-UVA to daily mechanical vibrations (0.15 g, 90 Hz, 15 min/day) for 7 days, both during quiescence and osteogenic commitment, to identify corresponding ultrastructural adaptations on cellular and molecular levels. During quiescence, mechanical vibrations significantly increased total actin content and …
Endogenous Heat Shock Protein Groel Of A. Actinomycetemcomitans Preferentially Targets Primary Human Cd8+ T Cells, Meli̇s Kant, Bünyami̇n Akgül, Ayten Nalbant Aldanmaz
Endogenous Heat Shock Protein Groel Of A. Actinomycetemcomitans Preferentially Targets Primary Human Cd8+ T Cells, Meli̇s Kant, Bünyami̇n Akgül, Ayten Nalbant Aldanmaz
Turkish Journal of Biology
Apoptosis can be used to manipulate host cells by bacterial products such as bacterial heat shock proteins (Hsp). One of the virulence factors of periodontal pathogen Aggregatibacter actinomycetemcomitans is heat shock protein GroEL (AaGroEL), which has been shown to interact with host cells. AaGroEL (Hsp64) also has the potential to modulate immune system cells. In this study we used endogenous AaGroEL protein as an antigen to study bacterial Hsp-induced apoptosis in different immune system cells. Human peripheral blood mononuclear cells and cell lines were cultured with different doses (50-1000 ng/mL) of endogenous AaGroEL at various time points. Apoptosis of the …
Improvement Of Atropine Production By Different Biotic And Abiotic Elicitors In Hairy Root Cultures Of Datura Metel, Zahra Shakeran, Mehrnaz Keyhanfar, Gholamreza Asghari, Mustafa Ghanadian
Improvement Of Atropine Production By Different Biotic And Abiotic Elicitors In Hairy Root Cultures Of Datura Metel, Zahra Shakeran, Mehrnaz Keyhanfar, Gholamreza Asghari, Mustafa Ghanadian
Turkish Journal of Biology
Hairy roots induced by Agrobacterium rhizogenes are distinguished by their high growth rate, genetic stability, and lateral branching. Datura metel is an important medicinal plant whose hairy roots are used for the production of atropine, a tropane alkaloid applied as an anticholinergic and parasympathetic agent. To increase the yield of atropine, biotic (Bacillus cereus and Staphylococcus aureus) and abiotic (AgNO_3 and nanosilver) elicitors were added to the hairy root cultures of D. metel and then analyzed through high-performance liquid chromatography. All the elicitors influenced biomass accumulation and atropine production. Among the tested elicitors, nanosilver was most effective in enhancing the …
Genotoxicity Of Metaphase-Arresting Methods In Barley, Aslihan Temel, Nermi̇n Gözükirmizi
Genotoxicity Of Metaphase-Arresting Methods In Barley, Aslihan Temel, Nermi̇n Gözükirmizi
Turkish Journal of Biology
Amiprophos-methyl (APM) and colchicine (COL) are therapeutic agents and are also used in plant science. Cell cycle synchronization (CCS) is mediated by hydroxyurea (HU) and APM. The genotoxic effects of APM are unclear. In order to assess the genotoxicity of APM separately and with HU and then compare it with COL, we treated 2-day-old barley (Hordeum vulgare 'Bornova-92') seedlings with 8 µM APM for 2 h, 1.25 mM COL for 18 h, or 1.25 mM HU for 18 h and 4 µM APM for 2 h, with a final ice-cold water incubation. Following treatment, seedlings were recovered without any substance …
Biosurfactant Production By Pleurotus Ostreatus In Submerged And Solid-State Fermentation Systems, Zülfi̇ye Veli̇oğlu, Razi̇ye Öztürk Ürek
Biosurfactant Production By Pleurotus Ostreatus In Submerged And Solid-State Fermentation Systems, Zülfi̇ye Veli̇oğlu, Razi̇ye Öztürk Ürek
Turkish Journal of Biology
Biosurfactants are surface active molecules that are produced by microorganisms. Their positive features attract the attention of many researchers compared to their chemically synthesized counterparts. This research studied the production of a biosurfactant by Pleurotus ostreatus in submerged fermentation (SmF) and solid-state fermentation (SSF), with and without shaking. Oil spreading efficiency, emulsification index, and surface tension were assessed during the study. The highest emulsification index (E_{24}) values of SmF, SSF with shaking, and SSF without shaking were 60 ± 5.0%, 29 ± 2.5%, and 42 ± 4.0%, respectively, and surface tension values were 40.7 ± 0.9, 30.8 ± 0.8, and …
Optimization And Characterization Of Cold-Active Endoglucanase Produced By Aspergillus Terreus Strain Akm-F3 Grown On Sugarcane Bagasse, Abhas Kumar Maharana, Pratima Ray
Optimization And Characterization Of Cold-Active Endoglucanase Produced By Aspergillus Terreus Strain Akm-F3 Grown On Sugarcane Bagasse, Abhas Kumar Maharana, Pratima Ray
Turkish Journal of Biology
The psychrotolerant microfungus Aspergillus terreus strain AKM-F3 (GenBank Accession No. KF417464) was investigated for the production of cold-active endoglucanase using sugarcane bagasse. The effects of different process parameters were studied for hyperproduction. Optimum production was found at 15 °C with an initial pH of 5.0 at 120 h. For enzyme production, 1 mM ZnCl_2, 0.01% aspartate, 2% lactose, and 3% NaNO_3 were regarded as potent inducers. The final production gave an activity 3.764-fold higher than the unoptimized conditions. The molecular weight of purified endoglucanase was 55 kDa, showing a 3.464-fold purification increase with a 23.925% yield having 75.279 U/mg of …