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Latest Trends, Green Aspects, And Innovations In Liquid-Phase--Based Microextraction Techniques: A Review, Erkan Yilmaz, Mustafa Soylak
Latest Trends, Green Aspects, And Innovations In Liquid-Phase--Based Microextraction Techniques: A Review, Erkan Yilmaz, Mustafa Soylak
Turkish Journal of Chemistry
Liquid-phase microextraction (LPME) methods including single-drop microextraction (SDME), hollow-fiber LPME (HF-LPME), and dispersive liquid-liquid microextraction (DLLME) have in the very short time since their invention grabbed the attention of scientists. Up to now, LPME methods have shown important innovations for the extraction and preconcentration of both inorganic and organic trace analytes from different matrices. These LPME methods offer unique advantages such as high preconcentration factor for target analytes in a single step, low cost, simplicity, excellent preconcentration capability, sample cleanup and integration of steps, and combined use with almost every analytical measurement technique. We describe the milestones and the combined …
A Taxonomic Study Of The Genus Hellenocarum H.Wolff (Umbelliferae-Apioideae)Based On Morphology, Fruit Anatomy, And Molecular Data, Ekaterina Andreevna Zakharova, Eugene Vasilyevich Kljuykov, Galina Viktorovna Degtjareva, Tahir Samigullin, Uliana Alekseevna Ukrainskaya, Stephen R. Downie
A Taxonomic Study Of The Genus Hellenocarum H.Wolff (Umbelliferae-Apioideae)Based On Morphology, Fruit Anatomy, And Molecular Data, Ekaterina Andreevna Zakharova, Eugene Vasilyevich Kljuykov, Galina Viktorovna Degtjareva, Tahir Samigullin, Uliana Alekseevna Ukrainskaya, Stephen R. Downie
Turkish Journal of Botany
Phylogenetic relationships among the species of Hellenocarum and its close allies (Umbelliferae-Apioideae) were investigated using nuclear (ITS, ETS) and plastid (psbA-trnH intergenic spacer) DNA sequences. The results obtained were supplemented with an examination of morphology from herbarium and field-collected materials, as well as details of fruit anatomy obtained from light and scanning electron microscopy. According to the molecular data, Hellenocarum is not monophyletic. In most molecular analyses, the genus comprises 2 disparate lineages, with each lineage supported by distinct morphological characters (e.g., structure of underground organs, shape of the umbel, petal color). The first lineage includes Hellenocarum multiflorum (the type …
Dichoropetalum Alanyensis (Apiaceae), A New Species From South Anatolia, Turkey, Bi̇lgehan Bi̇lgi̇li̇, Mehmet Sağiroğlu, Meryem Şeker, Hayri̇ Duman
Dichoropetalum Alanyensis (Apiaceae), A New Species From South Anatolia, Turkey, Bi̇lgehan Bi̇lgi̇li̇, Mehmet Sağiroğlu, Meryem Şeker, Hayri̇ Duman
Turkish Journal of Botany
Dichoropetalum alanyensis Bilgili, Sağıroğlu and H.Duman, a new species from South Anatolia in Turkey (Antalya-Alanya), is described and illustrated. It is closely related to D. Chryseum (Boiss. And Heldr. Ex Boiss.) Pimenov and Kljuykov, from which it differs mainly by its habit, basal leaves and lobes, and mericarp features. Diagnostic morphological characters are discussed. Notes are also given on its ecology and conservation status, together with scanning electron micrographs of mericarp surface sculpturing pattern. ITS sequences were used for DNA fingerprinting. The data were analyzed with the SEAVIEW package. Standardized data were used to generate a dendrogram that revealed the …
Isolation And Expression Analysis Of Three Different Flowering Genes(Ttlfy, Ttap1, And Ttap2) From An Unusual Legume Species, Thermopsis Turcica, Süleyman Cenkci̇, Mustafa Kargioğlu, Alperen Dedeoğlu, Büşra Kahraman, Yaşar Karakurt
Isolation And Expression Analysis Of Three Different Flowering Genes(Ttlfy, Ttap1, And Ttap2) From An Unusual Legume Species, Thermopsis Turcica, Süleyman Cenkci̇, Mustafa Kargioğlu, Alperen Dedeoğlu, Büşra Kahraman, Yaşar Karakurt
Turkish Journal of Botany
LEAFY (LFY), APETALA1 (AP1), and APETALA2 (AP2) genes encode three different transcription factors that control and regulate flower initiation and development in Arabidopsis. By using 3'- and 5'-RACE analysis, we isolated and sequentially characterized TtLFY (a LEAFY-like gene), TtAP1 (a MADS-box-like gene), and TtAP2 (an AP2/ERBF-like gene) in Thermopsis turcica, an unusual endemic legume species with three free carpellated flower structure. Semiquantitative RT-PCR analysis for 18 different vegetative and reproductive tissues of T. turcica indicated TtLFY transcripts mainly in the shoot tips and young floral buds and TtAP1 transcripts in the sepals and petals; however, TtAP2 transcripts were detected in …
First Molecular Records Of Potentially Harmful Planktonic Dinoflagellatesfrom The Southern Black Sea, Özgür Baytut, Cem Tolga Gürkanli, Eda Deni̇z, İbrahi̇m Özkoç, Ari̇f Gönülol
First Molecular Records Of Potentially Harmful Planktonic Dinoflagellatesfrom The Southern Black Sea, Özgür Baytut, Cem Tolga Gürkanli, Eda Deni̇z, İbrahi̇m Özkoç, Ari̇f Gönülol
Turkish Journal of Botany
The aim of this study was to identify dinoflagellate species from the Black Sea (Samsun coast) using conventional and molecular phylogenetic methods. Water samples were collected using a plankton net with 22 µm pores. Dinoflagellate cells were documented under a light microscope and were then isolated for the single-cell PCR procedure. Phylogenetic inference showed that the isolates collected from the study area were affiliated with various harmful taxa: Dinophysis acuta Ehrenberg and Phalacroma rotundatum (Claparéde & Lachmann) Kofoid & Michener from dinophysioids, and Prorocentrum compressum (Bailey) Abé ex Dodge, P. cordatum (Ostenfeld) Dodge, and P. micans Ehrenberg from prorocentroids. In …
Arbuscular Mycorrhizal And Dark Septate Endophyte Fungal Associations In Asparagus, Muthukumar Thangavelu, Muthuraja Raji
Arbuscular Mycorrhizal And Dark Septate Endophyte Fungal Associations In Asparagus, Muthukumar Thangavelu, Muthuraja Raji
Turkish Journal of Botany
We studied the arbuscular mycorrhizal (AM) and dark septate endophyte (DSE) fungal associations in five species of pot-grown Asparagus (A. aethiopicus, A. densiflorus, A. setaceus, A. racemosus, and A. umbellatus). Root colonization by AM and DSE fungi and AM spore numbers in the soil were assessed. AM fungal diversity indices like species richness, Shannon-Weiner index, Simpson's index, evenness, and Jaccard's index were determined. Relationships among the fungal and soil variables were also examined. All the species of Asparagus examined were colonized by both AM and DSE fungi, and these associations have been reported for the first time in four species. …
Mechanisms Of Mrna Polyadenylation, Hizlan Hincal Ağuş, Ayşe Eli̇f Erson Bensan
Mechanisms Of Mrna Polyadenylation, Hizlan Hincal Ağuş, Ayşe Eli̇f Erson Bensan
Turkish Journal of Biology
mRNA 3'-end processing involves the addition of a poly(A) tail based on the recognition of the poly(A) signal and subsequent cleavage of the mRNA at the poly(A) site. Alternative polyadenylation (APA) is emerging as a novel mechanism of gene expression regulation in normal and in disease states. APA results from the recognition of less canonical proximal or distal poly(A) signals leading to changes in the 3' untranslated region (UTR) lengths and even in some cases changes in the coding sequence of the distal part of the transcript. Consequently, RNA-binding proteins and/or microRNAs may differentially bind to shorter or longer isoforms. …
Synthetic Access To Some New Benzothiazole-Based 1,3,4-Thiadiazole And 1,3-Thiazole Derivatives, Kamal M. Dawood, Eman A. Ragab, Korany A. Ali
Synthetic Access To Some New Benzothiazole-Based 1,3,4-Thiadiazole And 1,3-Thiazole Derivatives, Kamal M. Dawood, Eman A. Ragab, Korany A. Ali
Turkish Journal of Chemistry
1-(Benzothiazol-2-yl)-3-phenylthiourea\textbf{ 2} was prepared and treated with hydrazonoyl chlorides \textbf{3a}--\textbf{e }to yield the corresponding 5-(benzothiazol-2-ylimino)-1,3,4-thiadiazole derivatives \textbf{6a}--\textbf{e}, respectively. Reaction of the thiourea derivative \textbf{2} with ethyl 2-chloro-3-oxobutanoate \textbf{9} afforded the corresponding 2-(benzothiazol-2-ylimino)thiazole-5-carboxylate derivative \textbf{11}. The newly synthesized heterocyclic derivatives were confirmed from their elemental and spectral analyses.
Synthesis And Evaluation Of Some Novel Thiazoles And 1,3-Thiazines As Potent Agents Against The Rabies Virus, Magda Abdalla, Sobhi Gomha, Mohamad Abd Elaziz, Nany Serag
Synthesis And Evaluation Of Some Novel Thiazoles And 1,3-Thiazines As Potent Agents Against The Rabies Virus, Magda Abdalla, Sobhi Gomha, Mohamad Abd Elaziz, Nany Serag
Turkish Journal of Chemistry
A series of novel thiazoles and 1,3-thiazine derivatives were synthesized in good yield via reaction of ethyl 3-(1-(2-thiocarbamoylhydrazono)ethyl)-1,5-diphenyl-1$H$-pyrazole-4-carboxylate with hydrazonoyl halides and arylidenemalononitriles, respectively. The structure of the newly synthesized products was elucidated via elemental analysis, spectral data, and alternative routes whenever possible. Moreover, the antiviral screening of the products was evaluated and the results revealed that some of them have strong to moderate potency against the rabies virus compared with the reference drug.
Synthesis, ($E)$/($Z)$-Isomerization, And Dna Binding, Antibacterial, And Antifungal Activities Of Novel Oximes And $O$-Substituted Oxime Ethers, Hati̇ce Başpinar Küçük, Ayşe Sergüzel Yusufoğlu, Leyla Açik, Betül Aydin, Leyla Arslan
Synthesis, ($E)$/($Z)$-Isomerization, And Dna Binding, Antibacterial, And Antifungal Activities Of Novel Oximes And $O$-Substituted Oxime Ethers, Hati̇ce Başpinar Küçük, Ayşe Sergüzel Yusufoğlu, Leyla Açik, Betül Aydin, Leyla Arslan
Turkish Journal of Chemistry
A series of novel positional oximes (2a-2d), $O$-methyl oxime ethers (3a-3d), and $O$-benzyl oxime ethers (4a-4d) were synthesized in high yields starting from their corresponding methyl 3-, 4-, 6-, and 13-keto tetradecanoates. The synthesized compounds were characterized by $^{1}$H NMR, $^{13}$C NMR, FT-IR, mass, and elemental analyses for their structures and $(E)$/$(Z)$ isomerizations. Their DNA binding abilities were investigated in vitro by agarose gel electrophoresis. The antibacterial and antifungal activities were tested also in vitro against eleven bacterial strains and three fungal strains. The relationship between the structure and the mentioned biological activities was discussed. Compound 2a showed good antibacterial …
Speciation Of Trace Metals And Metalloids By Solid Phase Extraction With Spectrometric Detection: A Critical Review, Ali̇ Rehber Türker
Speciation Of Trace Metals And Metalloids By Solid Phase Extraction With Spectrometric Detection: A Critical Review, Ali̇ Rehber Türker
Turkish Journal of Chemistry
Considerable interest is continuing to be shown in the speciation of metals, metalloids, and organometals due to the dependence of their toxicity and mobility on their chemical form. This review focuses on the speciation analysis of metals, metalloids, and organometals by solid phase extraction before spectrometric detection, and all aspects of analytical speciation such as determination of oxidation states and organometallic compounds. Some applications of speciation of metals and metalloids are also presented and discussed in this review. Fractionation, which is used sometimes as speciation, and chromatographic detection are not discussed. This review covers approximately the last four years (2012-March …
Epigenetic Regulation Of Brca1 Expression And Its Role Inbreast Cancer Stem Cell Development, Elena Shestakova
Epigenetic Regulation Of Brca1 Expression And Its Role Inbreast Cancer Stem Cell Development, Elena Shestakova
Turkish Journal of Biology
The tumor suppressor breast cancer 1, early onset (BRCA1) plays an important role in maintaining the genome integrity, actively participating in DNA repair via homologous recombination. The level of functional final BRCA1 protein is precisely regulated in cells. Epigenetic regulation of BRCA1 gene expression is one of the most important mechanisms for BRCA1 level fine-tuning. In this review the main epigenetic mechanisms of BRCA1 expression regulation are presented, including DNA methylation, histone covalent modifications, and regulation with transcription factors. Another important function of BRCA1 emerging in recent years is its role in the regulation of breast cancer stem cell development. …
Induced Pluripotent Stem Cells: Methods, Disease Modeling, And Microenvironment For Drug Discovery And Screening, Suresh Palamadai Krishnan
Induced Pluripotent Stem Cells: Methods, Disease Modeling, And Microenvironment For Drug Discovery And Screening, Suresh Palamadai Krishnan
Turkish Journal of Biology
The raging ethical, religious, moral, political, and safety issues pertaining to the use of embryonic stem cells for therapy and research have prompted researchers to develop possibly better alternatives that are more efficacious and serve multiple purposes. Induced pluripotent stem cells (iPSCs) developed by multiple methods provide an unparalleled source of cells that could be differentiated into progeny cells. In cell-based disease models the progeny cells mimic, at least in part, the pathology of the studied disease and are used to improve the mechanistic understanding of the pathology. Furthermore, these cells could be used to test the efficacy and safety …
Glioblastoma Stem Cells: A Therapeutic Challenge, Erdoğan Pekcan Erkan, Ufuk Vurgun, Reşat Serhat Erbayraktar, Zübeyde Erbayraktar
Glioblastoma Stem Cells: A Therapeutic Challenge, Erdoğan Pekcan Erkan, Ufuk Vurgun, Reşat Serhat Erbayraktar, Zübeyde Erbayraktar
Turkish Journal of Biology
The outcome for glioblastoma patients remains extremely poor, despite the advances in surgical and medical fields. It is hypothesized that glioblastoma progression, as well as tumor recurrence, is driven by a small number of cells called cancer stem cells (CSCs), which are characterized by their ability of self-renewal and proliferation, giving rise to progeny of transformation into multiple neuroepithelial lineages. Understanding the biology of CSCs is likely to explain why existing treatment strategies fail to affect the relatively quiescent and resistant CSC compartment. Here, we review the current knowledge on CSCs in glial tumors. In addition, we discuss the importance …
Current Status Of Stem Cell Therapy: Opportunities And Limitations, Elena Rusu, Laura Georgiana Necula, Ana Iulia Neagu, Mihail Alecu, Cristian Stan, Radu Albulescu, Cristiana Pistol Tanase
Current Status Of Stem Cell Therapy: Opportunities And Limitations, Elena Rusu, Laura Georgiana Necula, Ana Iulia Neagu, Mihail Alecu, Cristian Stan, Radu Albulescu, Cristiana Pistol Tanase
Turkish Journal of Biology
Over recent years stem cells have stood out as a promising tool for regenerative medicine, providing alternative therapeutic solutions for a large number of diseases. Many clinical trials using stem cells or induced pluripotent stem cells are focused on the repair and regeneration of various tissues and organs in degenerative diseases, whose current treatment only succeeds in slowing down the progression of the disease. Although the preliminary results are interesting, further studies are required in order to evaluate the safety and benefits of stem cell therapy, considering the teratoma development and ethical considerations in embryonic stem cell cases or reprogramming-induced …
Epigenetic Regulation Of Specific Transcription Factors In Osteogenicdifferentiation Of Mesenchymal Stem Cells, Milad Zadi Heydarabad, Mousa Vatanmakanian, Mina Nikasa, Majid Farshdousti Hagh
Epigenetic Regulation Of Specific Transcription Factors In Osteogenicdifferentiation Of Mesenchymal Stem Cells, Milad Zadi Heydarabad, Mousa Vatanmakanian, Mina Nikasa, Majid Farshdousti Hagh
Turkish Journal of Biology
An abundance of experiments have been performed to clarify the differentiation process of mesenchymal stem cells (MSCs). Osteogenic differentiation and in vitro conditions favoring these cell lineages have attracted the attention of many researchers. Moreover, the gene expression profile during MSC differentiation toward its main specialized cells (bone, cartilage, and adipose cells) has been mostly understood. In the last decades another layer of investigation has attempted to clarify the epigenetic mechanisms underlying MSC differentiation into its specialized cells. It has been shown that as MSCs progress through the differentiation process, more nonspecific genes undergo DNA methylation to prevent differentiation into …
The Heart Of The Matter: Cardiac Stem Cells, Esra Çağavi̇, Arzuhan Koç, Sevi̇lay Şahoğlu Göktaş
The Heart Of The Matter: Cardiac Stem Cells, Esra Çağavi̇, Arzuhan Koç, Sevi̇lay Şahoğlu Göktaş
Turkish Journal of Biology
Cardiovascular diseases are the primary cause of death in the world. Pharmacological and surgical approaches are the main treatment options for heart disease; however, heart transplantation may be the only option for advanced heart failure patients despite its limited nature. Recent advances enabled stem cell-based therapies to become a promising treatment approach for injured or weakened cardiac tissue. With the identification of resident and heart-specific stem cells in early 2000, new avenues of research have been opened to understand heart development, disease, and regeneration. In this review article, different cardiac stem cell subpopulations are classified and defined based on the …
Myelin Disorders And Stem Cells: As Therapies And Models, Fatmagül İlayda Aydinli, Eşref Çeli̇k, Burcu Kurt Vatandaşlar, Bi̇lal Ersen Kerman
Myelin Disorders And Stem Cells: As Therapies And Models, Fatmagül İlayda Aydinli, Eşref Çeli̇k, Burcu Kurt Vatandaşlar, Bi̇lal Ersen Kerman
Turkish Journal of Biology
Myelin disorders burden millions of people around the world, yet existing therapies are inadequate to cure them. Current remedies commonly treat the symptoms with minimal to no effect on the actual cause of the disorder. The basis and/or the mechanism of demyelination is not known for many of the disorders either. In recent years, stem cells of variable origin have been used in clinical trials as transplant agents to restore the defective biochemical process or the damaged tissue. We summarize the outcomes of these trials for demyelination disorders. The capability of reprograming mature cells into stem cells equips researchers with …
Human Adipose-Derived Stem Cells Differentiation Into Epidermal Cells And Interaction With Human Keratinocytes In Coculture, Alexandra Gaspar, Daniel Constantin, Ana-Maria Seciu, Lucia Moldovan, Oana Craciunescu, Elena Ganea
Human Adipose-Derived Stem Cells Differentiation Into Epidermal Cells And Interaction With Human Keratinocytes In Coculture, Alexandra Gaspar, Daniel Constantin, Ana-Maria Seciu, Lucia Moldovan, Oana Craciunescu, Elena Ganea
Turkish Journal of Biology
Current procedures used for skin injury reepithelization have yet to improve. At present, stem cell-based therapies provide great potential in repairing different damaged tissues due to the multipotent character of these cells. This study aimed to investigate the in vitro differentiation capacity of human adipose-derived stem cells (ASCs) into epidermal cells and to evaluate their interaction with HaCaT keratinocytes in coculture experimental models that mimicked the living environment of human skin. The mesenchymal phenotype of isolated stem cells was indicated by their spindle-shaped morphology and the expression of specific surface markers CD73, CD90, and CD105. After 21 days of cultivation …
Comparison Of The Osteogenic Differentiation Capacity Of Adipose Tissuederived Mesenchymal Stem Cells From Humans And Rats, Seçi̇l Erden Tayhan, Şeyma Taşdemi̇r, Sai̇me İsmet Deli̇loğlu Gürhan, Erol Mi̇r
Comparison Of The Osteogenic Differentiation Capacity Of Adipose Tissuederived Mesenchymal Stem Cells From Humans And Rats, Seçi̇l Erden Tayhan, Şeyma Taşdemi̇r, Sai̇me İsmet Deli̇loğlu Gürhan, Erol Mi̇r
Turkish Journal of Biology
Mesenchymal stem cells (MSCs) can be found in many types of adult tissues such as the bone marrow, adipose, placenta, liver, and periosteum. Recently, adipose tissue-derived MSCs (ADMSCs) have become one the most preferred MSC types because of their fast proliferation rate, abundance, and high plasticity for variable cell types. It is known that ADMSCs are able to differentiate into various cells, including osteoblasts, so they are quite promising for orthopedic clinical trials. The present study aimed to compare the osteogenic differentiation conditions of MSCs from human adipose tissue (hADMSC) and those of MSCs from rat adipose tissue (rADMSC). Therefore, …
Baculoviral Vector Loaded Mesenchymal Stem Cells As Efficient Gene Therapy Tools For Cancer Treatment, Sedef Hande Aktaş, Hakan Akbulut, Ayşe Eser Elçi̇n, Mahmut Parmaksiz, Arzu Keski̇n Aktan, Arzu Çöleri̇ Ci̇han, Yaşar Murat Elçi̇n, Fi̇kri̇ İçli̇
Baculoviral Vector Loaded Mesenchymal Stem Cells As Efficient Gene Therapy Tools For Cancer Treatment, Sedef Hande Aktaş, Hakan Akbulut, Ayşe Eser Elçi̇n, Mahmut Parmaksiz, Arzu Keski̇n Aktan, Arzu Çöleri̇ Ci̇han, Yaşar Murat Elçi̇n, Fi̇kri̇ İçli̇
Turkish Journal of Biology
Insufficient targeting of the therapeutic genes to tumor cells is one of the major reasons for failure in cancer gene therapy. Mesenchymal stem cells (MSCs) seem to be a good candidate as a carrier for gene therapy because of its selective tumor tissue-homing properties. In the current study, we constructed baculoviral vectors (BVs) carrying cytosine deaminase (CD) (BV-CD) or green fluorescence protein (GFP) genes (BV-GFP) and tested the transduction efficiency of the vectors in tumor and mesenchymal stem cells. We also tested the in vivo efficacy of the BV-CD vector in a colon cancer model. Our results showed that the …
Mediterranean Water Shrew (Neomys Anomalus): Range Expansion Northward, Linas Balciauskas, Laima Balciauskiene, Uudo Timm
Mediterranean Water Shrew (Neomys Anomalus): Range Expansion Northward, Linas Balciauskas, Laima Balciauskiene, Uudo Timm
Turkish Journal of Zoology
We identified three individuals of the Mediterranean water shrew (Neomys anomalus) in Estonia, expanding the known distribution range of the species to the north by 500 km from the most northern location in Lithuania and over 700 km from Poland. The identification of the species in Estonia, the most northern known locality, was based on the position of mental foramen, height of coronoid process (>4.3 mm), and Libois index (
A New Record Of The Genus Camerotrombidium Thor, 1936(Acari: Microtrombidiidae) From Turkey, İbrahi̇m Karakurt, Sevgi̇ Sevsay
A New Record Of The Genus Camerotrombidium Thor, 1936(Acari: Microtrombidiidae) From Turkey, İbrahi̇m Karakurt, Sevgi̇ Sevsay
Turkish Journal of Zoology
A newly recorded species for the Turkish fauna, Camerotrombidium rasum Berlese, 1910, is given based on adult females and larvae obtained from an adult female by experimental rearing in the laboratory. The genus Camerotrombidium is recorded for the first time from Turkey.
Numerical Classification And Ordination Of The Floodplain Forests In The Euxine Region Of Turkey, Ali̇ Kavgaci, Erkan Yalçin, Hasan Korkmaz
Numerical Classification And Ordination Of The Floodplain Forests In The Euxine Region Of Turkey, Ali̇ Kavgaci, Erkan Yalçin, Hasan Korkmaz
Turkish Journal of Botany
In this study, the phytosociological structure of floodplain forests in the Euxine region of Turkey was examined from a large-scale perspective. For this goal, all of the published relevés were analyzed using the numerical classification and ordination techniques, which are widely used in phytosociological studies all over the world. In summary, a syntaxonomical scheme for floodplain forests with 11 communities (five associations and one alliance were newly identified) was suggested as follows: Fraxino angustifoliae - Ulmetum laevis Slavi 1952 allerietosum petiolatae Kavgacı et al. Subass. Nov. Hoc loco; Fraxino angustifoliae - Ulmetum laevis Slavi 1952 junglandetosum regiae Kavgacı et al. …
Two New Achillea L. (Asteraceae) Species From Turkey, Zeki̇ Aytaç, Hayri̇ Duman, Murat Eki̇ci̇
Two New Achillea L. (Asteraceae) Species From Turkey, Zeki̇ Aytaç, Hayri̇ Duman, Murat Eki̇ci̇
Turkish Journal of Botany
Two new Achillea species, Achillea adenii Aytaç & M.Ekici and Achillea baltai H.Duman & Aytaç sp. nov. of sect. Santolinoidea DC. (Asteraceae), are described and illustrated as new species. A. adenii grows at Babadağ in Muğla Province (southwestern Anatolia), while A. baltai grows at Aladağlar in Niğde Province. Diagnostic morphological characters, which are useful in discriminating the two new species from their close relatives, A. sintenisii Hub.-Mor., A. sipikorensis Hausskn. & Bornm., and A. armenorum Boiss. & Hausskn., are presented. Some notes on the ecology, pollen and seed morphology, conservation status, and distributions of the new species and a key …
Ultrastructural Observations In Somatic Embryogenesis Of Natural Tetraploid Trifolium Pratense L., Hati̇ce Çölgeçen, Hati̇ce Nurhan Büyükkartal, Ufuk Koca Çalişkan
Ultrastructural Observations In Somatic Embryogenesis Of Natural Tetraploid Trifolium Pratense L., Hati̇ce Çölgeçen, Hati̇ce Nurhan Büyükkartal, Ufuk Koca Çalişkan
Turkish Journal of Botany
Previous reports of plant regeneration of natural tetraploid T. pratense L. `Elçi' could be realized only through the apical meristem calli. In order to proceed to the production stage, other regeneration methods need to be tried. Aseptic seedlings were used for the production of somatic embryos through various 2,4-D and kinetin trials. Nonuniform external callus cells with translucent cytoplasm were observed in various developmental stages of somatic embryos. Beneath these cells, there were uniformly aligned, dark-stained embryo cells with dense cytoplasm. Despite the similar developmental stages and cell characteristics of zygotic and somatic embryos, the walls of somatic embryo cells …
Genetic Relationships Among The Genera Cicer L., Lathyrus L., Lens Mill., And Vicia L., Together With Similarity Of Lens Taxa Based On Morphological And Aflp Markers, Zeynep Yildizdoğan, Cengi̇z İkten, Nedi̇m Mutlu, Cengi̇z Toker
Genetic Relationships Among The Genera Cicer L., Lathyrus L., Lens Mill., And Vicia L., Together With Similarity Of Lens Taxa Based On Morphological And Aflp Markers, Zeynep Yildizdoğan, Cengi̇z İkten, Nedi̇m Mutlu, Cengi̇z Toker
Turkish Journal of Botany
The molecular characterization of the seven taxa in the genus Lens Mill. with Vicia montbretii has not been performed, and species of the genera Vicia L., Lathyrus L., and Cicer L. using AFLP markers have not been elucidated so far. The objectives of the present study were the assessment of (i) genetic relationships among the genera Lens, Vicia, Lathyrus, and Cicer based on AFLP markers, (ii) relationships within the genus Lens including the accessions of L. lamottei Czefr., L. tomentosus Ladiz., and L. odemensis Ladiz., based on morphological and AFLP data, and (iii) position of the species Vicia montbretii (Fisch. …
Comparison Of Rdna Regions (Its, Lsu, And Ssu) Of Some Aspergillus, Penicillium, And Talaromyces Spp., Rasi̇me Demi̇rel
Comparison Of Rdna Regions (Its, Lsu, And Ssu) Of Some Aspergillus, Penicillium, And Talaromyces Spp., Rasi̇me Demi̇rel
Turkish Journal of Botany
Aspergillus, Penicillium, and Talaromyces spp. are commonly found worldwide and include industrially and medicinally important members. These genera are deeply related with life from soil to health hazards via their industrial products, surface proteins, biodiversity, food spoilage, mycotoxins, etc. These three genera progressively encounter important taxonomic revisions, such as the one fungus-one name concept, and various methods are available to establish their evolutionary and phylogenetic relationships. Besides morphological, microscopic, and extrolite analyses, nucleotide sequence analysis is the most preferred method. In particular, rDNA regions, such as the internal transcribed spacer (ITS), large subunit (LSU), and small subunit (SSU), are commonly …
Significance Of Using A Nonlinear Analysis Technique, The Lyapunov Exponent, On The Understanding Of The Dynamics Of The Cardiorespiratory System In Rats, Tamer Zeren, Mustafa Özbek, Neci̇p Kutlu, Mahmut Akilli
Significance Of Using A Nonlinear Analysis Technique, The Lyapunov Exponent, On The Understanding Of The Dynamics Of The Cardiorespiratory System In Rats, Tamer Zeren, Mustafa Özbek, Neci̇p Kutlu, Mahmut Akilli
Turkish Journal of Medical Sciences
Background/aim: Pneumocardiography (PNCG) is the recording method of cardiac-induced tracheal air flow and pressure pulsations in the respiratory airways. PNCG signals reflect both the lung and heart actions and could be accurately recorded in spontaneously breathing anesthetized rats. Nonlinear analysis methods, including the Lyapunov exponent, can be used to explain the biological dynamics of systems such as the cardiorespiratory system. Materials and methods: In this study, we recorded tracheal air flow signals, including PNCG signals, from 3 representative anesthetized rats and analyzed the nonlinear behavior of these complex signals using Lyapunov exponents. Results: Lyapunov exponents may also be used to …
Study Of Mouse Induced Pluripotent Stem Cell Transplantation Intowistar Albino Rat Cochleae After Hair Cell Damage*, Mustafa Kürşat Gökcan, Selçuk Mülazi̇moğlu, Emre Ocak, Pinar Can, Murat Çalişkan, Ömer Beşalti, Serpi̇l Di̇zbay Sak, Gülşah Kaygusuz
Study Of Mouse Induced Pluripotent Stem Cell Transplantation Intowistar Albino Rat Cochleae After Hair Cell Damage*, Mustafa Kürşat Gökcan, Selçuk Mülazi̇moğlu, Emre Ocak, Pinar Can, Murat Çalişkan, Ömer Beşalti, Serpi̇l Di̇zbay Sak, Gülşah Kaygusuz
Turkish Journal of Medical Sciences
Background/aim: As the regeneration capacity of hair cells is limited, inner ear stem cell therapies hold promise. Effects of mouse induced pluripotent stem cells (IPSCs) on Wistar albino rats (WARs) with hearing impairment were investigated. Materials and methods: Thirty-five adult WARs with normal hearing were divided into 4 groups. Excluding the study group (n = 15), the other three groups served as control groups for ototoxicity and IPSC injection models. IPSC injections were performed via cochleostomy after a retroauricular approach. Auditory functions were evaluated with auditory brainstem responses (ABRs) before and after the injections. After a final hearing assessment the …