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Transmission Ft-Ir Spectroscopic Analysis Of Human Kidney Stones In The Hyderabad Region Of Pakistan, Muhammad Hussan Khaskheli, Syed Tufail Hussain Sherazi, Huma Mazhar Ujan, Sarfaraz Ahmed Mahesar
Transmission Ft-Ir Spectroscopic Analysis Of Human Kidney Stones In The Hyderabad Region Of Pakistan, Muhammad Hussan Khaskheli, Syed Tufail Hussain Sherazi, Huma Mazhar Ujan, Sarfaraz Ahmed Mahesar
Turkish Journal of Chemistry
The purpose of the present study was to investigate the composition of kidney stones removed from human patients. Seventeen samples were collected after surgery (5 females and 12 males; age range 2 to 60 years). The samples were analyzed for composition by transmission Fourier transform infrared (FT-IR) spectroscopy. The 17 analyzed samples of kidney stones comprised 29.4% calcium oxalate, 29.4% uric acid, 29.4% calcium oxalate (CaOx)-uric acid, 5.9% phosphate, and 5.9% calcium oxalate-phosphate stones. The results of the study indicated that transmission FT-IR spectroscopy could be used as an environmentally friendly technique for rapid screening of kidney stones.
Optimization Of A New Cloud Point Extraction Procedure For The Selective Determination Of Trace Amounts Of Total Iron In Some Environmental Samples, Celal Duran, Duygu Özdeş, Eli̇f Çelenk Kaya, Hali̇t Kanteki̇n, Volkan Numan Bulut, Mehmet Tüfekçi̇
Optimization Of A New Cloud Point Extraction Procedure For The Selective Determination Of Trace Amounts Of Total Iron In Some Environmental Samples, Celal Duran, Duygu Özdeş, Eli̇f Çelenk Kaya, Hali̇t Kanteki̇n, Volkan Numan Bulut, Mehmet Tüfekçi̇
Turkish Journal of Chemistry
A new, simple, and rapid cloud point extraction (CPE) procedure system, combined with flame atomic absorption spectrometry (FAAS), was developed for selective separation, preconcentration, and determination of trace amounts of total iron in some environmental samples. The complexing agent, N,N'-(2,2'-(ethane-1,2-diylbis(oxy)) bis(ethane-2,1-diyl))bis(2-chloroacetamide) (EDBOCA), is selective at pH 5.0 for only Fe(II) and Fe(III) ions in the presence of Cu(II), Pb(II), Cd(II), Mn(II), Co(II), Cr(III), Cr(VI), Ni(II), Zn(II), Al(III), Mo(VI), Pd(II), Pt(IV), Au(III), and V(V) ions. The procedure is based on the complexation of Fe(III) ions with EDBOCA reagent in the presence of Triton X-114 (TX-114) as a non-ionic surfactant. The optimum …
The Reproductive Performance Of Wild And Hatchery-Reared Flounder, Platichthys Flesus Luscus, In The Southern Black Sea Coast, İlhan Aydin, Temel Şahi̇n, Hamza Polat, Erdi̇nç Güneş
The Reproductive Performance Of Wild And Hatchery-Reared Flounder, Platichthys Flesus Luscus, In The Southern Black Sea Coast, İlhan Aydin, Temel Şahi̇n, Hamza Polat, Erdi̇nç Güneş
Turkish Journal of Zoology
The spawning period, total fecundity, egg size, and fertilization and hatching rates of wild and hatchery-reared flounder, Platichthys flesus luscus, are investigated in this report. Wild and hatchery-reared broodstock consisted of 18 females (452.8 ± 193.2 g) and 17 females (138.9 ± 47.4 g), respectively. The spawning period lasted 79 days, from 22 January to 10 April. Mean total fecundity was 285 ± 157.9 × 10^3 per female for wild broodstock while it was 115.6 ± 53.6 × 10^3 eggs for hatchery-reared broodstock. The fertilization and hatching rates were 38.2 ± 17.0% and 62.3 ± 24.1% for wild and 45.3 …
Quality Parameters And Total Phenolic Content In Tomato Fruits Regarding Cultivar And Microclimatic Conditions, Nina Kacjan Marsic, Lea Gasperlin, Veronika Abram, Maja Budic, Rajko Vidrih
Quality Parameters And Total Phenolic Content In Tomato Fruits Regarding Cultivar And Microclimatic Conditions, Nina Kacjan Marsic, Lea Gasperlin, Veronika Abram, Maja Budic, Rajko Vidrih
Turkish Journal of Agriculture and Forestry
The aim of this research was to evaluate the color, firmness, and total phenolic (TP) content in tomatoes according to cultivar and growing conditions. Cultivars with oval, elongated, round, and cherry-shaped fruits of determinate tomato were grown in Mediterranean (Dragonja Valley) and continental regions. Experiments in the continental region were conducted outdoors (Ljubljana-field) and under a low tunnel (Ljubljana-tunnel). Results indicated that the color and firmness were significantly influenced by the typology of the cultivars and by the maturity stage associated with the climatic conditions. Oval, elongated, and cherry fruits had darker and more intensely red fruit skins, with significantly …
Field Evaluation Of Effective Microorganisms (Em) Application For Growth, Nodulation, And Nutrition Of Mung Bean, Arshad Javaid, Rukhsana Bajwa
Field Evaluation Of Effective Microorganisms (Em) Application For Growth, Nodulation, And Nutrition Of Mung Bean, Arshad Javaid, Rukhsana Bajwa
Turkish Journal of Agriculture and Forestry
Effective microorganisms (EM) is a commercial biofertilizer that contains a mixture of co-existing beneficial microorganisms collected from natural environments. Predominantly it consists of species of photosynthetic and lactic acid bacteria, yeast, and actinomycetes. The present study was conducted to evaluate the effect of EM application on growth, nodulation, yield, and nutrient uptake in mung bean [Vigna radiata (L.) Wilczek] var. NIAB Mung 98 under field conditions. Field soil was amended with farmyard manure at 20 t ha^{-1}, Trifolium alexandrinum green manure at 20 t ha^{-1}, and recommended (NPK) and half (½ NPK) doses of chemical fertilizers. EM was applied in …
Bioactivities Of Jimsonweed Extract, Datura Stramonium L. (Solanaceae), Against Tribolium Castaneum (Coleoptera: Tenebrionidae), Habib Abbasipour, Mohammad Mahmoudvand, Fahimeh Rastegar, Mohammad Hossein Hosseinpour
Bioactivities Of Jimsonweed Extract, Datura Stramonium L. (Solanaceae), Against Tribolium Castaneum (Coleoptera: Tenebrionidae), Habib Abbasipour, Mohammad Mahmoudvand, Fahimeh Rastegar, Mohammad Hossein Hosseinpour
Turkish Journal of Agriculture and Forestry
In this research, the efficacy of plant extract from Datura stramonium L. (Solanaceae) was tested against the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), for its toxic and antifeedant activity. The dried and powdered plant was extracted with 150 mL of absolute ethanol (99%, Merck) using a rotary evaporator extractor at 45 °C. Experiments were designed to measure the nutritional indices, such as relative growth rate (RGR), relative consumption rate (RCR), efficiency of conversion of ingested food (ECI), and feeding deterrence index (FDI). Treatments were evaluated by flour disk bioassay in the dark at 27 ± 1 °C and …
Determination And Pathogenicity Of The Bacterial Flora Associated With The Spruce Bark Beetle, Ips Typographus (L.) (Coleoptera: Curculionidae: Scolytinae), Hacer Muratoğlu, Kazim Sezen, Zi̇hni̇ Demi̇rbağ
Determination And Pathogenicity Of The Bacterial Flora Associated With The Spruce Bark Beetle, Ips Typographus (L.) (Coleoptera: Curculionidae: Scolytinae), Hacer Muratoğlu, Kazim Sezen, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
The Eurasian spruce bark beetle, Ips typographus (L.) (Coleoptera: Curculionidae: Scolytinae), is one of the most serious pests of spruce trees. We identified 8 bacterial isolates from this pest using conventional bacteriological tests (API 20E and API 50CH strips, and VITEK system (bioMerieux) analysis) and 16S rRNA gene sequence analysis. Based on these studies, all isolates could be identified to the genus or species level as Bacillus sphaericus (It1), Acinetobacter sp. (It2), Kluyvera cryocrescens (It3), Acinetobacter sp. (It4), Vagococcus sp. (It5), Acinetobacter sp. (It6), Proteus vulgaris (It7), and Serratia liquefaciens (It8). We also evaluated the pathogenicity of these bacteria on …
The Effects Of The Composition Of Growth Medium And Fermentation Conditions On The Production Of Lipase By R. Delemar, Ünsal Açikel, Mehtap Erşan, Yeşi̇m Sağ Açikel
The Effects Of The Composition Of Growth Medium And Fermentation Conditions On The Production Of Lipase By R. Delemar, Ünsal Açikel, Mehtap Erşan, Yeşi̇m Sağ Açikel
Turkish Journal of Biology
Lipases (triacylglycerol hydrolases) are hydrolytic enzymes that can catalyze the hydrolysis of the ester bond of long-chain acylglycerols at the oil-water interface. The present study investigated the effects of inducers, surface-active materials, activators, and inhibitors in the fermentation medium on lipase activity in Rhizopus delemar. In the presence of certain commercial oils and tributyrin as an inducer, lipase activity decreased in the order of sunflower oil > soybean oil > hazelnut oil > corn oil > tributyrin > olive oil. In addition, the effects of the stirring and aeration rates on lipase activity were investigated. To investigate the effect of surface-active materials on lipase activity …
Effects Of Vesicular Arbuscular Mycorrhiza Glomus Intraradices On Photosynthetic Pigments, Antioxidant Enzymes, Lipid Peroxidation, And Chromium Accumulation In Maize Plants Treated With Chromium, Raziyeh Rahmaty, Jalil Khara
Effects Of Vesicular Arbuscular Mycorrhiza Glomus Intraradices On Photosynthetic Pigments, Antioxidant Enzymes, Lipid Peroxidation, And Chromium Accumulation In Maize Plants Treated With Chromium, Raziyeh Rahmaty, Jalil Khara
Turkish Journal of Biology
Contamination of soil and ground water by chromium (Cr), due to its wide industrial use, has become a serious source of concern over the past decade. In this study a glasshouse experiment was conducted to investigate the effects of the mycorrhizal fungus Glomus intraradices on Cr toxicity in maize plants. Half of the plants were inoculated with the arbuscular mycorrhizal fungus (AMF). Cr was supplied in the form of potassium dichromate at 0.00, 0.10, 0.25, and 0.50 mM through irrigation water in a sand culture. At the end of the experiment, it was observed that Cr had significantly decreased the …
The Effect Of Diazinon On Egg Fertility And Development In Drosophila Melanogaster, Ayla Karataş, Zafer Bahçeci̇, Ensar Başpinar
The Effect Of Diazinon On Egg Fertility And Development In Drosophila Melanogaster, Ayla Karataş, Zafer Bahçeci̇, Ensar Başpinar
Turkish Journal of Biology
This study investigated the effects of diazinon, an organic phosphoric insecticide, on some developmental features of Drosophila melanogaster. The aim was to determine if any developmental inhibition occurs when diazinon is applied and if so, to determine which developmental stages are affected. Therefore, diazinon was applied to Drosophila melanogaster eggs, larvae, and adults, and its effects were observed. During the experiments 3 concentrations with values lower than LC_{50} were utilized (6 × 10^{-5}, 6 × 10^{-6}, 6 × 10^{-7} ppm). Diazinon solution was applied to Drosophila melanogaster by means of nutrition, adding it to the culture medium. The results show …
Influence Of Process Parameters On The Production Of Detergent Compatible Alkaline Protease By A Newly Isolated Bacillus Sp. Y., Shivakumar Srividya, Majumdar Mala
Influence Of Process Parameters On The Production Of Detergent Compatible Alkaline Protease By A Newly Isolated Bacillus Sp. Y., Shivakumar Srividya, Majumdar Mala
Turkish Journal of Biology
Detergent compatible alkaline protease production by a newly isolated Bacillus sp.Y from laundry soil was studied under shake flask conditions in growth medium comprised (g/L): peptone, 1; NaCl, 5; skim milk, 100; Na_2CO_3, 4. Different environmental process parameters such as fermentation period, initial pH, incubation temperature, and nutritional parameters such as supplementation of different starch sources and nitrogen sources were standardized for the maximum yield of alkaline protease. Rice flour showed an almost 2.2-fold increase in protease activity followed by wheat flour, which showed a 1.85-fold increase over the basal media. However, supplementation of potato starch, corn starch, and ragi …
An Entomopathogenic Bacterium, Pseudomonas Putida, From Leptinotarsa Decemlineata, Hacer Muratoğlu, Zi̇hni̇ Demi̇rbağ, Kazim Sezen
An Entomopathogenic Bacterium, Pseudomonas Putida, From Leptinotarsa Decemlineata, Hacer Muratoğlu, Zi̇hni̇ Demi̇rbağ, Kazim Sezen
Turkish Journal of Biology
The Colorado Potato Beetle (CPB), Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae), is a pre-eminent pest on solanaceous crops worldwide, and much research has focused on the different components of its variable population dynamics. L. decemlineata has developed resistance to insecticides used for its control. Consequently, alternative methods are needed urgently for the control of this pest. In this study, in order to find a more effective and safer biological control agent against L. decemlineata, we isolated a new bacterial isolate (Ld4) from L. decemlineata, and tested it for insecticidal effects on L. decemlineata larvae. Also, the insecticidal effect of Ld4 was …
The Protective Effect Of Boric Acid On Aluminum-Induced Hepatotoxicity And Genotoxicity In Rats, Hasan Türkez, Fati̇me Geyi̇koğlu, Suat Çolak
The Protective Effect Of Boric Acid On Aluminum-Induced Hepatotoxicity And Genotoxicity In Rats, Hasan Türkez, Fati̇me Geyi̇koğlu, Suat Çolak
Turkish Journal of Biology
The efficacy of boric acid (BA) was examined on liver marker enzymes in aluminum (Al)-treated rats. Also, a liver micronucleus assay was performed to evaluate the genotoxicity in hepatocytes. With these aims, Sprague-Dawley rats were randomly separated into 10 groups of 5 animals. Aluminum chloride (5 mg/kg body weight (b.w.) AlCl3) and BA (3.25, 13, 36 and 58.5 mg/kg b.w.) alone were administered with injections to the experimental animals. Furthermore, the animals were also treated with Al for 4 consecutive days followed by BA exposure for 10 days. The rats were anesthetized after Al and BA injections and the levels …
Antagonistic Potential Of Marine Actinobacteria Against Fish And Shellfish Pathogens, Selvakumar Dharmaraj
Antagonistic Potential Of Marine Actinobacteria Against Fish And Shellfish Pathogens, Selvakumar Dharmaraj
Turkish Journal of Biology
A total of 94 marine actinobacterial isolates were found to be associated with 4 sponges: Callyspongia diffusa, Mycale mytilorum, Tedania anhelans, and Dysidea fragilis. Among the cultures 7 strains showed antagonism against fish and shellfish pathogens. Analysis of morphological, physiological, biochemical, and protein profile characteristics suggested that the actinobacterial strains belonged to the genus Streptomyces. The initial screening of the isolates by the spot inoculation method exhibited antibacterial activity against Aeromonas hydrophila. In vitro screening of the submerged culture extracts showed positive inhibition against the fish and shellfish pathogens Aeromonas hydrophila, Serratia sp., and Vibrio spp. The screening of bioactive …
Determination Of Antimicrobial And Antiproliferative Activities Of The Aquatic Moss Fontinalis Antipyretica Hedw., Fi̇li̇z Savaroğlu, Cansu Fi̇li̇k İşçen, Ayşe Pinar Öztopcu Vatan, Selda Kabadere, Semra İlhan, Ruhi̇ Uyar
Determination Of Antimicrobial And Antiproliferative Activities Of The Aquatic Moss Fontinalis Antipyretica Hedw., Fi̇li̇z Savaroğlu, Cansu Fi̇li̇k İşçen, Ayşe Pinar Öztopcu Vatan, Selda Kabadere, Semra İlhan, Ruhi̇ Uyar
Turkish Journal of Biology
The purpose of the current study was to investigate the possible antimicrobial and anticancer properties of the aquatic moss Fontinalis antipyretica Hedw. (Fontinalaceae). We first obtained 8 different extracts (methanol; chloroform; acetone; ethyl acetate A, B, C, and D) by two different extraction processes. The antimicrobial activity of these extracts was then assessed by using the well diffusion method against 8 bacterial and 7 fungal strains. The results of antimicrobial studies showed that extracts of chloroform, acetone, and ethyl acetate A and C were active against almost all the species tested. The most effective extract was C, which was then …
Levels Of Phenolic Compounds And Their Effects On Antioxidant Capacity Of Wild Vaccinium Arctostaphylos L. (Qare-Qat) Collected From Different Regions Of Iran, Tahereh Hasanloo, Roshanak Sepehrifar, Homa Hajimehdipoor
Levels Of Phenolic Compounds And Their Effects On Antioxidant Capacity Of Wild Vaccinium Arctostaphylos L. (Qare-Qat) Collected From Different Regions Of Iran, Tahereh Hasanloo, Roshanak Sepehrifar, Homa Hajimehdipoor
Turkish Journal of Biology
Antioxidant activities in the leaves and fruits of wild Vaccinium arctostaphylos (locally named Qare-Qat) from different regions of Iran were evaluated using the stable 1, 1-diphenyl-2-picrylhydrazyl free radical and ferric reducing/antioxidant power methods. Variations in antioxidant activity, total phenolic, anthocyanin, and flavonoid content in fruit samples among genotypes was much greater than the variation observed between growing seasons, indicating that genetics plays a more important role than growing season in V. arctostaphylos fruits. The data indicated that the fruits of the Kelardasht genotype had the highest total phenolic content [42.73 ± 1.5 mg gallic acid equivalents (GAE) g^{-1} DW], anthocyanin …
Changes In The Hemolymph Total Protein Of Galleria Mellonella (Lepidoptera: Pyralidae) After Parasitism And Envenomation By Pimpla Turionellae (Hymenoptera: Ichneumonidae), Olga Sak, Ekrem Ergi̇n, Fevzi̇ Uçkan, David B. Rivers, Ayli̇n Er
Changes In The Hemolymph Total Protein Of Galleria Mellonella (Lepidoptera: Pyralidae) After Parasitism And Envenomation By Pimpla Turionellae (Hymenoptera: Ichneumonidae), Olga Sak, Ekrem Ergi̇n, Fevzi̇ Uçkan, David B. Rivers, Ayli̇n Er
Turkish Journal of Biology
Venom from the endoparasitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) contains a mixture of biologically active components, which display potent paralytic, cytotoxic, and cytolytic effects towards hosts. Here, we further investigate if parasitism or envenomation by P. turionellae alters total protein of its host Galleria mellonella L. (Lepidoptera: Pyralidae). Various venom concentrations representing doses previously determined to yield host responses yet fall below the calculated LD99 were used for pupae and larvae. Parasitization was only assayed for host pupa since P. turionellae females normally parasitize host prepupae and pupae in nature. Hemolymph total protein concentration remained relatively steady at all doses …
Biochemical Identification And Numerical Taxonomy Of Aeromonas Spp. Isolated From Food Samples In Turkey, Belgi̇n Erdem, Ergi̇n Kari̇ptaş, Eli̇f Çi̇l, Kami̇l Işik
Biochemical Identification And Numerical Taxonomy Of Aeromonas Spp. Isolated From Food Samples In Turkey, Belgi̇n Erdem, Ergi̇n Kari̇ptaş, Eli̇f Çi̇l, Kami̇l Işik
Turkish Journal of Biology
Numerical taxonomy was used to analyze phenotypic data obtained from 126 new isolates of Aeromonas strains taken from red meat, raw chicken, minced meat, and fish samples. Each strain was tested for 86 characters but only the final data including 63 characters were analysed using the S_{SM} coefficients and the UPGMA clustering algorithm. At S_{SM} values of => 83%, the strains clustered into 10 aggregate groups consisting of 7 major (5 and up strains) and 3 minor (2-4 strains), and 5 single member clusters, each of which was identified as A. hydrophila, A. caviae, and A. sobria, respectively. It was …
Numerical Classification Of Streptomycetes Isolated From Karstic Caves In Turkey, Mustafa Yamaç, Kami̇l Işik, Nevzat Şahi̇n
Numerical Classification Of Streptomycetes Isolated From Karstic Caves In Turkey, Mustafa Yamaç, Kami̇l Işik, Nevzat Şahi̇n
Turkish Journal of Biology
In the present study, a total of 290 actinomycetes isolates were obtained from 19 karstic caves in Turkey. Out of these, 131 were chosen for numerical classification studies according to their morphology and pigmentation properties. Representative isolates were compared with the reference Streptomyces strains based on their various taxonomic characteristics such as biochemical, physiological, nutritional, and morphological, totalling 134 unit characters. The data were evaluated using simple matching coefficients (S_{SM}), and clustering achieved using the Complete algorithm by X-Taxon and NTSys-pc programs. The test strains were assigned to 9 major (5-11 strains), 18 minor (2-4 strains), and 31 single membered …
Influence Of Different Sterilization Methods On Callus Initiation And Production Of Pigmented Callus In Arnebia Densiflora Ledeb., Hati̇ce Çölgeçen, Ufuk Koca Çalişkan, Gülnur Toker
Influence Of Different Sterilization Methods On Callus Initiation And Production Of Pigmented Callus In Arnebia Densiflora Ledeb., Hati̇ce Çölgeçen, Ufuk Koca Çalişkan, Gülnur Toker
Turkish Journal of Biology
We analyzed the effects of sodium hypochlorite and Plant Preservative Mixture (PPM) on surface sterilization. We also examined the effects that the addition of an antibiotic-antimycotic solution to the culture medium had on callus induction. Explants were initially sterilized with different concentrations of sodium hypochlorite and cultured on MS media containing kinetin (0.29 \muM) and naphthalene acetic acid (NAA, 10 \muM). No calluses were produced, either because of contamination of the explants, or loss of explants as a result of the high levels of sodium hypochlorite. The application of PPM and antibiotics at different concentrations reduced contamination and led to …
Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu
Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu
Turkish Journal of Biology
This is the first report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. The isolated strain was cultivated for lipase production in shake flasks at 30 °C for 7 days. For lipase production, it was determined that the best carbon source was glucose and the best nitrogen source was peptone. Maximum biomass was produced at a concentration of 1.25 g/L, in 7 days. The optimum pH and temperature for activity of the enzyme were 8.5 and 40 °C, respectively. The lipase was stable at a pH range of 8.0-10.0 and …
Acid Proteinase Enzyme Activity In Candida Albicans Strains: A Comparison Of Spectrophotometry And Plate Methods, Sevi̇m Akçağlar, Beyza Ener, Okan Töre
Acid Proteinase Enzyme Activity In Candida Albicans Strains: A Comparison Of Spectrophotometry And Plate Methods, Sevi̇m Akçağlar, Beyza Ener, Okan Töre
Turkish Journal of Biology
In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. The proteinase enzyme activities of 30 C. albicans strains isolated from the bloodstream and sterile body fluids were investigated (15 colonizations, 15 infections). The spectrophotometric and plate methods were used to determine proteinase activity. The correlations between the 2 methods were compared. This study indicates …
Characterization Of Poly Phenol Oxidase In Two In Vitro Regenerated Cultivars Of Mucuna: Mucuna Pruriens L. And Mucuna Prurita H., Raghavendra Sathyanarayana, Vadlapudi Kumar, Chapeyil Kumaran Ramesh, Mahadevappa Parmesha, Mahaboob Habeebulla Moinuddin Khan
Characterization Of Poly Phenol Oxidase In Two In Vitro Regenerated Cultivars Of Mucuna: Mucuna Pruriens L. And Mucuna Prurita H., Raghavendra Sathyanarayana, Vadlapudi Kumar, Chapeyil Kumaran Ramesh, Mahadevappa Parmesha, Mahaboob Habeebulla Moinuddin Khan
Turkish Journal of Biology
Polyphenol oxidases (PPOs EC 1.14.18.1) were isolated from Mucuna pruriens and Mucuna prurita and confirmed as tyrosinases involved in L-DOPA production. PPOs were extracted by using a 0.05 M phosphate buffer, pH 7.0. The purified enzyme was resolved into a single band by PAGE, the enzyme was confirmed as PPO by activity staining, and SDS-PAGE analysis revealed that the purified PPO of both the species is a tetramer. Substrate specificity experiments were carried out with catechol, L-DOPA, L-tyrosine, and p-cresol. Of these, catechol was evaluated as the most suitable substrate based on the determined K_{m} and V_{max} values. The optimum …