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Full-Text Articles in Entire DC Network
Detection Of Infectious Bronchitis Virus In Tracheal Organ Cultures Using The Immunoperoxidase Technique, Ayşi̇n Şen, Gürsel Sönmez, Vi̇ldan Caner, M. Özgür Özyi̇ği̇t
Detection Of Infectious Bronchitis Virus In Tracheal Organ Cultures Using The Immunoperoxidase Technique, Ayşi̇n Şen, Gürsel Sönmez, Vi̇ldan Caner, M. Özgür Özyi̇ği̇t
Turkish Journal of Veterinary & Animal Sciences
The applicability of the immunoperoxidase (IP) technique in unfixed-fresh tracheal organ cultures (TOCs) was investigated for rapid detection of infectious bronchitis virus (IBV). For this purpose, TOCs prepared from one-day-old SPF chicks were inoculated with IBV M41 strain in serial dilutions. IBV antigen detected by IP technique in TOCs 18 h postinoculation. IP staining was observed before siliostasis in all dilutions. The IP technique was also applied to detect IBV antigen in formalin-fixed, paraffin-embedded sections of TOCs inoculated with IBV M41 strain. Viral antigen was also detected in these sections. Histopathological examinations of IBV M41 infected tracheal rings showed loss …
Studies On Early Diagnosis Of Rhodococcus Equi Infection By Elisa In Foals From Racehorse Breeding Studfarms In The Marmara Region, N. Yakut Özgür, Serkan İki̇z, Arzu Funda Bağcigi̇l, Babür Cari̇oğlu, Ömer Akay, Ati̇lla Ilgaz, Shinji Takai
Studies On Early Diagnosis Of Rhodococcus Equi Infection By Elisa In Foals From Racehorse Breeding Studfarms In The Marmara Region, N. Yakut Özgür, Serkan İki̇z, Arzu Funda Bağcigi̇l, Babür Cari̇oğlu, Ömer Akay, Ati̇lla Ilgaz, Shinji Takai
Turkish Journal of Veterinary & Animal Sciences
Due to the recent increasing mortality rates in 1- to 4-month-old Thoroughbred foals with Rhodococcus equi infection in Turkey, serum samples from 454 foals between 30 and 60 days on studfarms of the Marmara Region, where the Thoroughbred foal population is concentrated, were investigated by ELISA for early diagnosis of the infection. The Tween 20-extracted antigen of the R. equi ATCC 6939 strain was used in the ELISA. Two hundred and nine (46%) of the 454 foals were found to be ELISA-positive. Optical density (OD) values of 0.3 (positive limit) or higher were detected in 43% of foals aged 30-40 …
Identification Of Chicken Originated Campylobacter Coli And Campylobacter Jejuni By Polymerase Chain Reaction (Pcr), Hasan Basri̇ Ertaş, Burhan Çeti̇nkaya, Adi̇le Muz, Hasan Öngör
Identification Of Chicken Originated Campylobacter Coli And Campylobacter Jejuni By Polymerase Chain Reaction (Pcr), Hasan Basri̇ Ertaş, Burhan Çeti̇nkaya, Adi̇le Muz, Hasan Öngör
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to isolate Campylobacter species from the intestines and livers of chicken and to identify Campylobacter coli and Campylobacter jejuni by both conventional methods and Polymerase Chain Reaction (PCR). Four specific primers derived from the ceuE gene present in the genomes of C. coli and C. jejuni were used for PCR identification. In the examination of 150 intestine and liver samples by culture and PCR, 25 (16.6%) and 32 (21.3%) were identified as C. coli and C. jejuni, respectively. It was concluded that the PCR assay used in this study may successfully be applied for …
Cover And Contents
Turkish Journal of Veterinary & Animal Sciences
No abstract provided.
The Effects Of Bsa And Fcs On In Vitro Maturation Of Bitch Oocytes, Mi̇that Evecen, Alper Baran, Serhat Alkan, Ö. Banu Özdaş, Çağatay Tek
The Effects Of Bsa And Fcs On In Vitro Maturation Of Bitch Oocytes, Mi̇that Evecen, Alper Baran, Serhat Alkan, Ö. Banu Özdaş, Çağatay Tek
Turkish Journal of Veterinary & Animal Sciences
The effects of protein sources added to the medium for maturation of bitch oocytes were investigated. Ovaries from spayed bitches were transported to the laboratory in PBS at 38 ºC within an hour. Recovered oocytes were divided into three groups and left for 72 h maturation at 38.5 ºC under an atmosphere of 5% CO_2 and high humidity. Group 1 (Control): TCM 199 medium, Group 2 (BSA): TCM 199 medium supplemented with 0.3% BSA, Group 3 (FCS): TCM 199 medium supplemented with 5% FCS. At the end of the maturation period, all oocytes were denuded from cumulus cells by pipetting …
Detection Of Cattle Infected With Theileria Annulata In Fields By Nested Pcr, Ifat And Microscopic Examination Of Blood Smears, Zati̇ Vatansever, Serpi̇l Nalbantoğlu
Detection Of Cattle Infected With Theileria Annulata In Fields By Nested Pcr, Ifat And Microscopic Examination Of Blood Smears, Zati̇ Vatansever, Serpi̇l Nalbantoğlu
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to diagnose Theileria annulata infected cattle by microscopy, IFAT and nested PCR and to compare the sensitivity and specificity of these diagnostic methods. A total of 147 blood and sera samples were collected from healthy cattle in four localities of Polatlı district, Ankara, where tropical theileriosis is prevalent. Examination results revealed positivity rates of 31.3%, 44.9% and 61.2% for microscopy, IFAT and nested PCR, respectively. It was shown that nested PCR is more sensitive and specific when compared to microscopical examination and serological findings. It was also shown that nested PCR is more sensitive …
Ultrasonographic Evaluation Of Liver Damage And Obstruction Of The Biliary Tract In Experimentally Bile Duct Ligated Dogs, Remzi̇ Gönül, Yücel Meral, Abdullah Kayar, Utku Bakirel, Mehmet Erman Or, Gülbi̇n Şennazli, Aydin Gürel, Zi̇ya Aslan
Ultrasonographic Evaluation Of Liver Damage And Obstruction Of The Biliary Tract In Experimentally Bile Duct Ligated Dogs, Remzi̇ Gönül, Yücel Meral, Abdullah Kayar, Utku Bakirel, Mehmet Erman Or, Gülbi̇n Şennazli, Aydin Gürel, Zi̇ya Aslan
Turkish Journal of Veterinary & Animal Sciences
The liver damage and obstructions of the biliary tract were evaluated ultrasonographically in experimentally bile duct ligated dogs. Seven healthy dogs of different breeds were used. The common bile duct was double ligated at the duodenal region after clinical and ultrasonographic examinations. After that, clinical examinations, biochemical analyses and examinations of the liver, gallbladder and biliary tract were done ultrasonographically over 6 weeks. All dogs were necropsied and macroscopic and histologic alterations were determined at the end of the study. It was determined that the levels of serum ALT, ALP, GGT and T. Bilirubin were (p < 0.001-0.05) statistically significantly increased after the operation in all dogs. As a result of ultrasonographic examinations, it was determined that the bile ducts and gallbladder were distended, the neck of the gallbladder was larger and more tortuous than normal, hypoechoic sludge was prominent in the gallbladder, and perivascular, multifocal hyperechoic areas appeared in the liver parenchyma toward the end of the study. These alterations were confirmed macroscopically and microscopically at the end of the autopsy in all dogs. We consider that as well as blood serum parameters, ultrasonographic examinations are useful in the early diagnosis of biliary diseases and in differentiating them from hepatic diseases.
Effect Of Force Feeding With Various Energy Levels Of Diets On Fattening Performance And Fatty Liver Production Of Adult Geese, Ö. Hakan Muğlali, Ahmet Ergün, Cansu Ağca, Ayhan Güler, Kemal Küçükersan, Mehmet Orman, İlkay Yalçinkaya, Pinar Saçakli
Effect Of Force Feeding With Various Energy Levels Of Diets On Fattening Performance And Fatty Liver Production Of Adult Geese, Ö. Hakan Muğlali, Ahmet Ergün, Cansu Ağca, Ayhan Güler, Kemal Küçükersan, Mehmet Orman, İlkay Yalçinkaya, Pinar Saçakli
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to determine the effect of force feeding on the fattening performance, fatty liver production and some blood parameters of adult geese. The level of cholesterol, triglyceride, HDL and LDL in plasma lipids and the hepatic enzymes SGOT, SGPT and ALP were analysed during different periods of the study. While the initial plasma lipid fractions, cholesterol, triglyceride, HDL and LDL, of the control group, group I and group II were 245.42 ± 16.79, 370.14 ± 31.42, 61.42 ± 6.37 and 11.90 ± 1.90; 233.85 ± 8.54, 374.85 ± 37.98, 61.57 ± 4.38 and 10.42 ± 2.22; …
Periodical Determination Of Some Microbiological Characteristics Of Sucuk Samples Produced At Some Big Meat Plants In The City Of Afyon, Ahmet Hi̇lmi̇ Çon, Mebrure Doğu, Hüsnü Yusuf Gökalp
Periodical Determination Of Some Microbiological Characteristics Of Sucuk Samples Produced At Some Big Meat Plants In The City Of Afyon, Ahmet Hi̇lmi̇ Çon, Mebrure Doğu, Hüsnü Yusuf Gökalp
Turkish Journal of Veterinary & Animal Sciences
Some microbiological characteristics (TAMB, Enterobacteriaceae, yeast-mold, S. aureus, C. perfringens numbers) of sucuk samples, which were the products of the 5 major meat plants and marketed in the province of Afyon, were determined. Six samples were taken periodically from 5 different firms, so total of 30 samples were analysed, over 3 months. Minimum, maximum and average values for the different microbiological counts were as follows respectively, 3.0x10^4 CFU/g, 2.2x10^8 CFU/g and 2.9x107 CFU/g for TAMB;
Cloning And Expression Of The Gene Encoding Industrial \Alpha-Amylase From Bacillus Subtilis Orba97 In Escherichia Coli And Bacillus Subtilis Yb886, Numan Özcan
Turkish Journal of Veterinary & Animal Sciences
The \alpha-amylase encoding gene from Bacillus subtilis ORBA97 strain was cloned and expressed in Escherichia coli with the plasmid vector called pUC18 as well as in the \alpha-amylase negative Bacillus subtilis YB886 strain by using the pUB110 vector. The specific activity of the enzyme was determined to be 5.052 µmol (mg protein min)^{-1}. Zymogram analysis revealed no sign of protease degradation in the YB886 strain. The optimum temperature and pH of the enzyme were found to be 50°C and 5 respectively. Relative enzyme activity decreased to 36% of the original activity when it was subjected to a temperature of 90°C …
Investigation On Serum Calcium (Ca), Phosphorus (P) And Alkaline Phosphatase (Ap) Levels In Cows With Retained Placenta, Yaşar Akar, Hami̇t Yildiz, Hakan Keçeci̇, Muhterem Aydin
Investigation On Serum Calcium (Ca), Phosphorus (P) And Alkaline Phosphatase (Ap) Levels In Cows With Retained Placenta, Yaşar Akar, Hami̇t Yildiz, Hakan Keçeci̇, Muhterem Aydin
Turkish Journal of Veterinary & Animal Sciences
In this study, calcium (Ca), phosphorus (P) and alkaline phosphatase (AP) levels of the blood serum of cows with retained placenta (RP) were measured and the role of these parameters on the etiology of the RP was investigated. A total of 28 different ages and breeds of cows, of which 14 (group 1) had retained placenta and 14 (group 2) discharged fetal membranes (within 3-8 hours of delivery) were used as the material of the study. Additionally, these two groups were divided into two subgroups, according to the sexes of their calves, and three subgroups called groups A (3 years), …
A Study On The Determination Of Some Parameters Of The Scorpion Fish (Scorpaena Porcus Linne., 1758) Caught By Bottom Nets In The Area Of Sinop In Terms Of Fishery Biology, Habi̇l Uğur Koca
Turkish Journal of Veterinary & Animal Sciences
In the present research, which was carried out during the period of June 1996 to May 1997 in order to determine some population parameters of the scorpion fish (Scorpaena porcus Linne., 1758) in the Sinop region, a total of 633 individuals were investigated according to age, length, weight and sex composition, growth rate, the relationship of length-weight, Von Bertalanffy Growth Formula and the condition factor. The sex ratio of the stock was determined to be 43.76% female and 56.24% male and it was found that the fish were 1-6 years old. In the research, the relationship of length-weight was found …
The Effect Of Zinc On Body Weight And Some Serum Enzymes In Rats, Süleyman Korkut Tekeli̇, Nezi̇r Yaşar Toker, Fatma Tekeli̇
The Effect Of Zinc On Body Weight And Some Serum Enzymes In Rats, Süleyman Korkut Tekeli̇, Nezi̇r Yaşar Toker, Fatma Tekeli̇
Turkish Journal of Veterinary & Animal Sciences
100 male Wistar albino rats were used in this study. After the adaptation period, the rats were divided into 5 equal groups. The experimental period lasted 22 days. Food and water were given ad libitum. 0 ppm, 100 ppm, 500 ppm, 1,000 ppm and 2,000 ppm zinc were given by nasogastric probe to the control, experiment-1, experiment-2, experiment-3 and experiment-4 groups respectively, on days 1, 6, 11, 16, and 21 of the experimental period. The animals were weighed and blood samples were taken on days 2, 7, 12, 17, 22 of the experimental period. AST, ALT and ALP levels in …
An Investigation On Milk Yield And Reproductive Performance Of Holstein Friesian Cows In Kocas State Farm 2. Reproductive Traits, Serdar Duru, Erdoğan Tuncel
An Investigation On Milk Yield And Reproductive Performance Of Holstein Friesian Cows In Kocas State Farm 2. Reproductive Traits, Serdar Duru, Erdoğan Tuncel
Turkish Journal of Veterinary & Animal Sciences
This research was conducted to determine the characteristics of reproductive performance of Holstein Friesian cows raised at Kocas state farm in Central Anatolia. The material of the research was 362 dairy cows raised from 1988 to 1995 at Kocas State farm. Statistical analysis of records was conducted with the Minitab version 9.2 computer program. Significant differences between groups were determined by the method of Least Significant Difference (LSD). The general mean values of first breeding age, age at first calving, reproductive efficiency, calving interval, gestation length, dry period, service period and insemination number per conception were 18.04±0.10 months, 27.70±0.11 months, …
Survival Rate, Growth Characteristics And Some Body Measurements Of Akkaraman And Sakız (Chios) X Akkaraman F_1 Lambs, Necmetti̇n Ünal
Survival Rate, Growth Characteristics And Some Body Measurements Of Akkaraman And Sakız (Chios) X Akkaraman F_1 Lambs, Necmetti̇n Ünal
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to investigate the survival rate, growth characteristics and some body measurements of Akkaraman and Sakız (Chios) x Akkaraman F_1 lambs. The Akkaraman breed has a fat tail and is raised in Central Anatolia, while Sakız has a long, but low fat tail and is raised in coastal areas of Western Anatolia. In the study, fertility results of Akkaraman x Akkaraman and Sakız x Akkaraman (SA) were determined to be 83.67 and 75.44% for birth rates, and 1.12 and 1.16 for litter size, respectively. The survival rates of Akkaraman and SA F_1 lambs at weaning (90 …
Effect Of Oxytocin Administered In The Early Stage Of The Oestrous Cycle On The Length Of The Oestrous Cycle And Levels Of Serum Progesterone, Plasma A Vitamin And Ss-Carotene In Cows, Hami̇t Yildiz, Mehmet Çay
Effect Of Oxytocin Administered In The Early Stage Of The Oestrous Cycle On The Length Of The Oestrous Cycle And Levels Of Serum Progesterone, Plasma A Vitamin And Ss-Carotene In Cows, Hami̇t Yildiz, Mehmet Çay
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to analyse the effect of the application of oxytocine in the early stage of the oestrous cycle on the levels of serum progesterone and plasma vitamin A and ß-carotene during the cycle. Five Swiss-Brown cows were used during two oestrous cycles. Blood samples were collected during the first cycle starting after synchronization and then a total daily dose of 100 I.U. oxytocine was applied subcutanously as a half dose at 12 hour intervals on days on 4, 5 and 6 of the second cycle. During the same cycle, blood samples were again collected, from …
Some Microbiological And Chemical Properties Of Pastırma Produced Using Potassium Nitrate And Starter Culture, Muhammet İrfan Aksu, Mükerrem Kaya
Some Microbiological And Chemical Properties Of Pastırma Produced Using Potassium Nitrate And Starter Culture, Muhammet İrfan Aksu, Mükerrem Kaya
Turkish Journal of Veterinary & Animal Sciences
In this study, pastırma samples were produced using potassium nitrate and commercial starter culture (Staphylococcus carnosus + Lactobacillus pentosus). The meat for pastırma was dry cured with NaCl, glucose and saccarose together with KNO_3. The use of starter culture in pastırma production had a significant effect on counts of total aerobic mesophilic bacteria, Micrococcus/Staphylococcus and lactic acid bacteria. The maximum counts of microorganisms were found in the 2^{nd} stage of the drying process. There was no significant difference between yeast mould counts in control and starter added samples. Nitrite and nitrate residues were lower in commercial starter added samples than …
Cloning And Expression Of Bacteriophage T4 Lysozyme Gene (Gene E) In Escherichia Coli Via Pcr Amplification, Ali̇ İrfan Güzel, Numan Özcan, Hali̇l Kasap
Cloning And Expression Of Bacteriophage T4 Lysozyme Gene (Gene E) In Escherichia Coli Via Pcr Amplification, Ali̇ İrfan Güzel, Numan Özcan, Hali̇l Kasap
Turkish Journal of Veterinary & Animal Sciences
Lysozyme (EC 3.2.1.17) is an enzyme that catalyzes the hydrolysis of \beta(1-->4) glycosidic bonds between N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), which are present in peptidoglycan heteropolymers of the prokaryotic cell wall. The purpose of this study was the cloning and expression of the bacteriophage T4 lysozyme gene (gene e) via PCR amplification in Escherichia coli using the pUC18 and pRS416 vectors. PCR amplification is carried out by using the primers having Bam HI recognition sites at their 5' ends. After digesting the vectors and PCR product with Bam HI endonuclease, two recombinant plasmids were constructed by ligation reaction …
Lethal Chlorine Concentrations For The Pearl Oyster Pinctada Radiata (Leach, 1814), Müni̇r Zi̇ya Lugal Göksu, Fatma Çevi̇k, Özlem Findik
Lethal Chlorine Concentrations For The Pearl Oyster Pinctada Radiata (Leach, 1814), Müni̇r Zi̇ya Lugal Göksu, Fatma Çevi̇k, Özlem Findik
Turkish Journal of Veterinary & Animal Sciences
In this study, the lethal chlorine concentrations (LC) and residual chlorine concentrations in water for a fouling organism, the pearl oyster Pinctada radiata (Leach, 1814) [Avicula], were investigated during May 1999-April 2000. The study was carried out under laboratory conditions at 20ºC. Acute toxicity tests were conducted under a flow through system by using continuous application of chlorine according to bioassay test methods. Test solutions were made by using an appropriate amount of sodium hypochlorite (NaOCl^{-}). According to the results, the 24h-LC_{50} was 1.75 mgl^{-1} and the residual chlorine was 0.47 mgl^{-1} for LC_{50} of Pinctada radiata.
Estimate Of Variance Components With Different Methods For Growth Traits Of Lambs And Reproductive Characters Of Awassi And Red Karaman Ewes, Nuri̇ni̇sa Esenbuğa, Hayri̇ Dayioğlu
Estimate Of Variance Components With Different Methods For Growth Traits Of Lambs And Reproductive Characters Of Awassi And Red Karaman Ewes, Nuri̇ni̇sa Esenbuğa, Hayri̇ Dayioğlu
Turkish Journal of Veterinary & Animal Sciences
In this study, the effects of environmental factors on flock production characteristics of Awassi and Red Karaman ewes reared at the Research and Application Farm of the Agriculture Faculty were studied, and variance component estimates were made using different methods. Variance component estimates for both breeds for the number of pregnant ewes, ewes lambing, lambs born and lambs weaned per ewe mated, lambs born, lambs weaned, lambs surviving 120 and 210 days per ewe lambing, birth weight, weaning weight, daily weight gain until weaning, weight at the end of pasture and daily weight gain in pasture lambs were made using …
Some Mineral Substance Levels In Serum Of Sheep And Goat Grazing In Hatay Region, Suat Erdoğan, Yaşar Ergün, Zeynep Erdoğan, Tünay Kontaş
Some Mineral Substance Levels In Serum Of Sheep And Goat Grazing In Hatay Region, Suat Erdoğan, Yaşar Ergün, Zeynep Erdoğan, Tünay Kontaş
Turkish Journal of Veterinary & Animal Sciences
This study was carried out on 93 sheep and 92 goats which were all clinically healthy and raised in the pasture conditions in the Hatay region. Blood samples were collected from the jugular vein between June and July (1999) in Altınözü, Reyhanlı, Yayladağı, Samandağı and Dörtyol province. Serum samples were analysed by atomic absorption spectrophotometry for copper and zinc. Sodium and potassium levels were determined by flame photometry, calcium levels were quantified by Eppendorf photometry, magnesium was measured by spectrophotometry and selenium was determined by fluorometry. In the Hatay region, Mg 1.08±0.02 mmol/L, Ca 2.38±0.06 mmol/L, Na 149.07±2.06 mmol/L, K …
Caviar Production From Flathead Grey Mullet (Mugil Cephalus, Lin. 1758) And The Determination Of Its Chemical Composition And Roe Yield, Gülgün F. Ünal Şengör, Aykut Ci̇haner, Nuray Erkan, Özkan Özden, Candan Varlik
Caviar Production From Flathead Grey Mullet (Mugil Cephalus, Lin. 1758) And The Determination Of Its Chemical Composition And Roe Yield, Gülgün F. Ünal Şengör, Aykut Ci̇haner, Nuray Erkan, Özkan Özden, Candan Varlik
Turkish Journal of Veterinary & Animal Sciences
The aim of this research is to determine the yield and chemical composition of the caviar produce from flathead grey mullet (Mugil cephalus, Lin.1758) in the Sufa lagoon. Acording to the results of our research 21.98% of the fish with an average live weight of 1,243.63 ± 322.44 g and 49.11 ± 3.83 cm length contains with roe. Salting the caviar caused a weight loss of 35%. As a result of salting, the salt content of the product was raised to 4.61% and as a result of waxing this reached a maximum of 7.04%. In all chemical composition parameters salted …
Research On Various Testing Procedures And Different Methods Of Estimating Lactation Milk Yield In Dairy Cattle, Atti̇la Kaya, Can Uzmay, Yavuz Akbaş, İbrahi̇m Kaya, Sencer Tümer
Research On Various Testing Procedures And Different Methods Of Estimating Lactation Milk Yield In Dairy Cattle, Atti̇la Kaya, Can Uzmay, Yavuz Akbaş, İbrahi̇m Kaya, Sencer Tümer
Turkish Journal of Veterinary & Animal Sciences
This research was carried out to determine the effect of various milk sampling intervals and different methods on the accuracy of estimation of lactation milk yield in dairy cattle. Individual daily milk weights of 599 Holstein, Simmental and Brown Swiss lactations from the Aegean Agricultural Research Institute were used. It was assumed that test-day milk yields were obtained by regular and irregular intervals of 28 d and 56 d, with the irregular intervals having a standard deviation of 5 d. From test-day milk yields, 305 d milk yields were estimated by the Holland (Lactation yield = mean of test day …
Cover And Contents
Turkish Journal of Veterinary & Animal Sciences
No abstract provided.
Introduction Of The Precise Ts Measurement For Fisheries Acoustics, Kouichi Sawada, Yoshimi Takao, Yoichi Miyanohana, H. Tuncay Kinacigi̇l
Introduction Of The Precise Ts Measurement For Fisheries Acoustics, Kouichi Sawada, Yoshimi Takao, Yoichi Miyanohana, H. Tuncay Kinacigi̇l
Turkish Journal of Veterinary & Animal Sciences
Two methods to measure target strength (TS) are introduced and compared with each other. One is the suspension method, and the other is the split-beam method. Each of them is representative of control and in situ methods, respectively. In addition, a morphological investigation of a swimbladder in a pressure tank, and the development of an instrumented package of acoustic and optical systems, are also introduced. The former is to reveal the degree of TS variation by the vertical movement of fish, and the latter is to obtain precise TS and fish behavior by the echo trace analysis method.
Expression Of Subunits Of Ampa-Type (Ionotropic) Glutamate Receptors In The Rat Spinal Cord, Mümtaz Nazli, Zafer Soygüder, Ahmet Çarhan
Expression Of Subunits Of Ampa-Type (Ionotropic) Glutamate Receptors In The Rat Spinal Cord, Mümtaz Nazli, Zafer Soygüder, Ahmet Çarhan
Turkish Journal of Veterinary & Animal Sciences
The distribution of ionotropic glutamate receptor subunits (GluR1-4), considered a-amino-3-hydroxy-5-methyl-4-isaxole propionic acid type (AMPA-type), was investigated in the rat spinal cord by immunocytochemistry. Different distributions of the AMPA-type glutamate receptor subunits were observed in the spinal cord. Immunolabelling with antibodies to both GluR1 and GluR2/3 revealed intensive staining in the dorsal horn, while staining for GluR2/3 and GluR4 revealed dense motor neurones in the ventral horn. These results suggest that in the rat spinal cord AMPA-type glutamate receptors vary in composition according to the region where they are expressed.
Membrane Specific Carbonic Anhydrase (Ca-Iv) Expression In Bovine Lung: The Effects Of Alcoholic And Non-Alcoholic Drinks, Nazan Demi̇r, Hayrunni̇sa Nadaroğlu, Yaşar Demi̇r
Membrane Specific Carbonic Anhydrase (Ca-Iv) Expression In Bovine Lung: The Effects Of Alcoholic And Non-Alcoholic Drinks, Nazan Demi̇r, Hayrunni̇sa Nadaroğlu, Yaşar Demi̇r
Turkish Journal of Veterinary & Animal Sciences
Carbonic anhydrase (CA) (carbonate hydrolyase: E. C. 4.2.1.1) from bovine lung was purified by a new method and characterized. The purification level was 4306-fold. The optimum temperature for maximum enzyme activity was 37.5°C. The optimum pH was 7.4, varying between 3.5 and 10.0. SDS-polyacryamide gel electrophoresis (3-10% discontinuous SDS-PAGE) showed two distinct bands for CA-IV. The molecular weights of the enzymes were found to be approximately 54.000 and 29.000, respectively. V_{max} and K_{M} values were calculated with p-nitrophenyl acetate as substrate (166.7 µmol/L*min, 5.59*10_{-2} M, respectively). Changes in enzyme activity were determined in the presence of some chemicals: sulfanilamide, NaN_3, …
Incidence And Pathologic Studies On Liver Telangiectasis In Beef Cattle, Ayhan Atasever, Sevi̇l Atalay Vural, Şenay Berki̇n
Incidence And Pathologic Studies On Liver Telangiectasis In Beef Cattle, Ayhan Atasever, Sevi̇l Atalay Vural, Şenay Berki̇n
Turkish Journal of Veterinary & Animal Sciences
In this study, 3470 livers of beef cattle slaughtered by the Ankara Meat and Fish Company were inspected. In these animals, 82 livers had telangiectasis, and the incidence of lesions was 2.3%. They were characterized grossly by single or multiple red-brown foci, generally 1 to 5 mm in diameter, and occasionally larger. They were located on the parietal surface of the lobus dexter or rarely scattered throughout the organ. Microscopically, dilated sinusoids filled with erythrocytes were observed in initial lesions; the distortion, atrophy and necrosis of hepatocytic cordons were seen in advanced cases. There was no correlation between liver abscesses …
The Prevalence Of Bovine Viral Diarrhoea Virus (Bvdv) Infections In Cattle And Existence Of Persistently Infected Cattle In The Trakya Region, Seyyal Ak, İbrahi̇m Firat, H. Hakan Bozkurt, Veli̇ Gülyaz, Kemal Ak
The Prevalence Of Bovine Viral Diarrhoea Virus (Bvdv) Infections In Cattle And Existence Of Persistently Infected Cattle In The Trakya Region, Seyyal Ak, İbrahi̇m Firat, H. Hakan Bozkurt, Veli̇ Gülyaz, Kemal Ak
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the prevalence of BVDV infection and existence of persistently infected (PI) cattle in the Trakya region. In total, 260 samples of leukocytes were isolated from dairy cows (65 samples) and breeding bulls (65 samples) and also from cows and bulls in slaughterhouses (65 samples from each). After two blind passages in foetal bovine kidney (FBK) cell culture, they were screened for BVDV antigen with indirect immunoperoxidase labelling. For detecting PI cattle among BVDV positive live animals, new samples were taken and labelled, approximately two months after the first sampling. It was found …
Analyses Of Carbohydrates Of Apolipoprotein E In Vldl And Idl+Ldl Of Rat Serum, Tayfun Güldür, Yuan C. Lee
Analyses Of Carbohydrates Of Apolipoprotein E In Vldl And Idl+Ldl Of Rat Serum, Tayfun Güldür, Yuan C. Lee
Turkish Journal of Veterinary & Animal Sciences
Carbohydrate compositions of apolipoprotein E (apo E) isolated from rat serum very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) + low density lipoproteins (LDL) were analyzed and compared. Rat serum VLDL and IDL+LDL were isolated by a discontinuous density gradient ultracentrifugation for 7.9x107g.min. Following delipidization, apolipoproteins were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Apo E polypeptides were eluted by diffusion in 100 mM PBS buffer (pH 7) containing 0.05% SDS. Sugars were derivatized with 1-phenyl-3-methyl-5-pyrazolone (PMP) and subjected to high performance liquid chromatography (HPLC) analysis. N-acetylneuraminic acids of apo E were derivatized with 4,5-methylenedioxy-1,2-phenylene-diamine dihydrochloride …