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Isospora Species (I. Canaria, Isospora Sp.) In Canaries (Serinus Canarius, Linnaeus), Cem Ecmel Şaki̇, Edi̇p Özer
Isospora Species (I. Canaria, Isospora Sp.) In Canaries (Serinus Canarius, Linnaeus), Cem Ecmel Şaki̇, Edi̇p Özer
Turkish Journal of Veterinary & Animal Sciences
Of 64 canaries with diarrhea in Elazığ Province between May 2002 and May 2006, 18 (28.1%) had Isospora oocysts in their feces. It was determined that the oocysts, examined morphologically before and after sporulating, were Isospora canaria and Isospora sp. Among the infected animals, 8 (44.4%) were concurrently infected with both species. Total numbers of I. canaria oocysts were greater than those of Isospora sp. A total of 2 canaries showing severe clinical signs were necropsied. Liver, spleen, and lung preparations and peripheral blood smears of the necropsied animals were examined for Atoxoplasma, and no development forms were seen. For …
Hyperactivation Of The Hypothalamo-Pituitary-Adrenocortical Axis In Streptozotocin Diabetic Rats: Effect Of Tinospora Cordifolia (Willd.) And Insulin Therapy, Nagaraja Puranik, Sheela Devi Devi, Kararshaha Kammar
Hyperactivation Of The Hypothalamo-Pituitary-Adrenocortical Axis In Streptozotocin Diabetic Rats: Effect Of Tinospora Cordifolia (Willd.) And Insulin Therapy, Nagaraja Puranik, Sheela Devi Devi, Kararshaha Kammar
Turkish Journal of Medical Sciences
In the present study, we investigated the extent to which changes in corticosterone level and food and water intake behavior occur in diabetes and explored the effect of Tinospora cordifolia extracts and insulin therapy in streptozotocin-induced diabetic rats. Materials and methods: Female albino rats of the inbred Wistar strain (body weight: 180-210 g) were used in this study. Experimental diabetes was induced by injecting freshly prepared streptozotocin once at the dose of 55 mg/kg body weight intravenously in 0.1 M citrate buffer, pH 4.5. The drug was administered orally for 10 days and 30 days in different groups containing 6 …
Cytotoxic Effect Of Cigarette Smoke Condensate On Mice And Rat Mesenchymal Stem Cells And Hela Cells, Aftab Ahmad, Abdul Rauf Shakoori
Cytotoxic Effect Of Cigarette Smoke Condensate On Mice And Rat Mesenchymal Stem Cells And Hela Cells, Aftab Ahmad, Abdul Rauf Shakoori
Turkish Journal of Medical Sciences
Aim: To study the effect of cigarette smoke condensate (CSC) on HeLa cells and mesenchymal stem cells (MSCs) of mice (mMSC) and rats (rMSC). Cigarette smoking results in the deaths of millions of people annually. The constituents of cigarette smoke produce oxidants in the cells that result in inflammation, carcinogenesis, and apoptosis. Materials and methods: CSC was collected on filter paper and dissolved in dimethyl sulfoxide, and 10-100 µg/mL was added to HeLa cells, mMSCs, and rMSCs in a 96-well plate. The cytotoxic effect of CSC was measured after 48 h using a neutral red uptake assay. Images of the …
Investigation Of Genetic Variation In Tunisian Date Palm (Phoenix Dactylifera L.) Cultivars Using Issr Marker Systems And Their Relation With Fruit Characteristics, Hammadi Hamza, Mohamed Ali Benabderrahim, Mokhtar Elbekkay, Guasmi Ferdaous, Tebra Triki, Ali Ferchichi
Investigation Of Genetic Variation In Tunisian Date Palm (Phoenix Dactylifera L.) Cultivars Using Issr Marker Systems And Their Relation With Fruit Characteristics, Hammadi Hamza, Mohamed Ali Benabderrahim, Mokhtar Elbekkay, Guasmi Ferdaous, Tebra Triki, Ali Ferchichi
Turkish Journal of Biology
Phoenix dactylifera L. was introduced in Tunisia a very long time ago and plays an important socioeconomic role, especially in the south of the country. Genetic diversity and relationships among 26 cultivars were assessed with 7 ISSR primers. A total of 43 amplified bands were obtained. Principal component analyses based on Nei genetic distances showed no geographical separation with the exception of the Dhahbi cultivar, which has a limited geographical distribution. A group of cultivars that are also phonetically clustered was distinguished. These cultivars have a common maturity period and a common fruit consistency. The Mantel test emphasizes a significant …
Effects Of Arbuscular Mycorrhizal Inoculation On Biochemical Parameters In Capsicum Annuum Grown Under Long Term Salt Stress, Fazi̇let Özlem Çeki̇ç, Serpi̇l Ünyayar, İbrahi̇m Ortaş
Effects Of Arbuscular Mycorrhizal Inoculation On Biochemical Parameters In Capsicum Annuum Grown Under Long Term Salt Stress, Fazi̇let Özlem Çeki̇ç, Serpi̇l Ünyayar, İbrahi̇m Ortaş
Turkish Journal of Botany
Salt stress is an important environmental stress. Plants cope with salt stress with different strategies. In this study the effects of 2 different arbuscular mycorrhiza species (Glomus mosseae and G. intraradices) on some biochemical parameters in pepper plants (Capsicum annuum L. cv. Cumaovası) exposed to long term salt stress were studied. It was found that mycorrhizal inoculation increased RWC, P, total chlorophyll, and carotenoid content of pepper plants during salt application. The enzyme activities changed depending on the enzyme and salt stress application. The lowest MDA content was found in the plants inoculated with G. intraradices; however, there was no …
The Bryophyte Flora Of The Western Part Of The Küre Mountains (Bartın, Kastamonu), Turkey, Muhammet Ören, Güray Uyar, Tamer Keçeli̇
The Bryophyte Flora Of The Western Part Of The Küre Mountains (Bartın, Kastamonu), Turkey, Muhammet Ören, Güray Uyar, Tamer Keçeli̇
Turkish Journal of Botany
This study presents the bryophyte flora of the western part of the Küre Mountains, situated in A2 square in the grid system adopted by Henderson, and entirely within the Euro-Siberian phytogeographic region. A total of 1545 bryophyte specimens were collected from the research area in 2008 and 2009. According to the results of this study, 272 taxa belonging to 146 genera and 60 families were recorded. Of these, 2 taxa are hornworts, 53 taxa are liverworts, and 217 taxa are mosses. Among them, 38 taxa are new records for A2 and 4 taxa [Leiocolea badensis (Gottsche) Jörg., Cephaloziella rubella (Ness) …
Antioxidative And Physiological Responses Of Two Sunflower (Helianthus Annuus) Cultivars Under Peg-Mediated Drought Stress, Mehmet Cengi̇z Baloğlu, Musa Kavas, Gülsüm Aydin, Hüseyi̇n Avni̇ Öktem, Ayşe Meral Yücel
Antioxidative And Physiological Responses Of Two Sunflower (Helianthus Annuus) Cultivars Under Peg-Mediated Drought Stress, Mehmet Cengi̇z Baloğlu, Musa Kavas, Gülsüm Aydin, Hüseyi̇n Avni̇ Öktem, Ayşe Meral Yücel
Turkish Journal of Botany
Drought stress is one of the most important yield-reducing factors in crop production. Sunflower, an oilseed crop, is severely affected by abiotic stress. In this study, 2 sunflower cultivars (Musala and Aydın) were evaluated in terms of various biochemical and physiological responses under 2 different polyethylene glycol-mediated drought stress conditions. Stress-determining parameters such as malondialdehyde (MDA), hydrogen peroxide (H_{2}O_{2}), and proline contents were determined. Both cultivars showed similar responses at osmotic potentials of both -0.4 and -0.8 MPa. Aydın was less affected than Musala under these stress conditions. MDA, H_{2}O_{2}, and proline levels were similar at both -0.4 and -0.8 …
Identification Of 23 Specific Nucleotide Patterns In The Hn Gene Of Newcastle Disease Viruses Isolated From Iran, Majid Esmaelizad, Mehdi P. Ashtiani, Saber Jelokhani Niaraki, Khadije Hashemnejad
Identification Of 23 Specific Nucleotide Patterns In The Hn Gene Of Newcastle Disease Viruses Isolated From Iran, Majid Esmaelizad, Mehdi P. Ashtiani, Saber Jelokhani Niaraki, Khadije Hashemnejad
Turkish Journal of Biology
Newcastle disease is considered the most contagious poultry disease and may cause severe economic loss in the poultry industry. The present study was undertaken to focus on molecular and phylogenetic analyses of the hemagglutinin-neuraminidase (HN) gene in the Iranian isolates. The nucleotide sequence of the HN (780-903 nt) coding region of 6 Newcastle disease viruses (NDVs), isolated from various outbreaks in Iran, was determined and aligned with previously published sequences of 26 NDV isolates in GenBank; the resulting alignment was then analyzed in terms of genetic variation. The number of nucleotide sequence variations presented by each of the isolates indicated …
Properties Of Biological Activity Of Ten Wild Almond (Prunus Amygdalus L.) Species, Ali Jahanban Esfahlan, Rashid Jamei
Properties Of Biological Activity Of Ten Wild Almond (Prunus Amygdalus L.) Species, Ali Jahanban Esfahlan, Rashid Jamei
Turkish Journal of Biology
To compare the antioxidant and antiradical activity of phenolic extracts of different species of wild almond kernels (including the brown skin), 10 wild almond species were selected from Shahindezh and Qasemloo Valley in West Azarbaijan Province, Iran. The fruits of these almonds were collected, their kernels were separated from their hulls and shells and then ground, and methanolic extracts were prepared from the kernels. The total phenolic and flavonoid contents were determined using the Folin-Ciocalteu method and a colorimetric method, respectively. The extracts were also evaluated on their reducing power and their capacity to scavenge for DPPH radicals. Significant differences …
The Histopathologic Effects Of Morus Alba Leaf Extract On The Pancreas Of Diabetic Rats, Jamshid Mohammadi, Prakash R. Naik
The Histopathologic Effects Of Morus Alba Leaf Extract On The Pancreas Of Diabetic Rats, Jamshid Mohammadi, Prakash R. Naik
Turkish Journal of Biology
Many traditional treatments have been recommended in the alternative system of medicine for the treatment of diabetes mellitus. Mulberry (Morus alba) is a nontoxic natural therapeutic agent shown to possess hypoglycemic, hypotensive, and diuretic properties. The purpose of this study was to examine the histopathologic effects of Morus alba leaf extract on the pancreas of diabetic rats. The animals were treated with mulberry leaf extract at dosages of 400 and 600 mg/kg body weight for 35 days. The various parameters studied included blood glucose, the relative body weight of the pancreas, the diameter of islets, and the number of \beta …
Genetic Diversity Of Natural Cyclamen Alpinum Populations, Belgi̇n Göçmen Taşkin, Ni̇lden Vardareli̇, Ersi̇n Doğaç, Ramazan Mammadov, Vatan Taşkin
Genetic Diversity Of Natural Cyclamen Alpinum Populations, Belgi̇n Göçmen Taşkin, Ni̇lden Vardareli̇, Ersi̇n Doğaç, Ramazan Mammadov, Vatan Taşkin
Turkish Journal of Biology
The focus of this study was Cyclamen alpinum (formerly C. trochopteranthum). Habitat fragmentation, environmental degradation, and overharvesting of tubers have exerted pressure on native populations of this valuable ornamental species. Although the entire Cyclamen genus is in the Convention on International Trade in Endangered Species of Wild Fauna and Flora-Appendix II (CITES II), no species has yet been red-listed. Estimating the level and distribution of genetic variation in populations of rare and endemic species is important for conserving genetic diversity within a species in the context of well-developed conservation strategies. Currently, DNA markers are the most effective means used to …
A Study On The Characterization And Pathogenicity Of Bacteria From Lymantria Dispar L. (Lepidoptera: Lymantriidae), İsmai̇l Demi̇r, Emi̇ne Eryüzlü, Zi̇hni̇ Demi̇rbağ
A Study On The Characterization And Pathogenicity Of Bacteria From Lymantria Dispar L. (Lepidoptera: Lymantriidae), İsmai̇l Demi̇r, Emi̇ne Eryüzlü, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
Gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), is one of the most serious pests affecting fruit, forest, industrial, and foliage plants worldwide. The aim of this study was to isolate and characterize pathogenic bacteria from this pest and determine their pathogenicity. L. dispar larvae were collected from various populations in the forest areas of the Black Sea region of Turkey in May and June of 2008 and 2009, and 9 culturable bacterial isolates were obtained from these larvae. Morphological and physiological characteristics of bacterial isolates were determined by conventional and routine techniques, and biochemical properties and metabolic enzyme profiles were …
Colonization Of Mycobacterium Phlei In The Rhizosphere Of Wheat Grown Under Saline Conditions, Dilfuza Egamberdieva
Colonization Of Mycobacterium Phlei In The Rhizosphere Of Wheat Grown Under Saline Conditions, Dilfuza Egamberdieva
Turkish Journal of Biology
One of the natural reservoirs of potentially human-pathogenic bacteria is believed to be the rhizosphere. The aim of the present work was to test nontuberculous mycobacterium Mycobacterium phlei MbP18 for its ability to colonize the rhizosphere of wheat and to evaluate its effect on plant growth under saline conditions. In competitive wheat root tip colonization assays, M. phlei MbP18 showed poor competitive colonization of the wheat rhizosphere compared to the reference strain. The strain produced lipase, amylase, cellulase, and pectinase and grew well in the presence of high salt (up to 4% NaCl) and at high temperatures (up to 40 …
Biomarkers Of Petroleum Contamination In The Digestive Gland Of Perna Viridis (L.), Xivanand Narayan Verlecar, Karmabeer Jena, Somshekher Raghoba Desai, Priya Brata Das, Gagan Bihari Nityananda Chainy
Biomarkers Of Petroleum Contamination In The Digestive Gland Of Perna Viridis (L.), Xivanand Narayan Verlecar, Karmabeer Jena, Somshekher Raghoba Desai, Priya Brata Das, Gagan Bihari Nityananda Chainy
Turkish Journal of Biology
Oxidative stress responses were evaluated in the digestive gland tissue of Perna viridis exposed to water-accommodated fractions (WAFs) of petrol and diesel at 0.5% and 5% concentrations over 5-, 10-, and 15-day periods. Increased lipid peroxidation, protein carbonyl, DNA integrity, and hydrogen peroxide in WAF-exposed specimens are indicative of oxidative damage due to dissolved petroleum extracts. A consistent increase in superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, reduced glutathione, and ascorbic acid in WAF-exposed specimens signify the importance of antioxidants in protecting the cell against the oxidative damage arising from petroleum contamination. The responses of field specimens collected from …
Characterization Of Affinity Tag Features Of Recombinant Tetrahymena Thermophila Glutathione-S-Transferase Zeta For Tetrahymena Protein Expression Vectors, Cem Özi̇ç, Muhi̇tti̇n Arslanyolu
Characterization Of Affinity Tag Features Of Recombinant Tetrahymena Thermophila Glutathione-S-Transferase Zeta For Tetrahymena Protein Expression Vectors, Cem Özi̇ç, Muhi̇tti̇n Arslanyolu
Turkish Journal of Biology
Glutathione S-transferase (GST) is the one of most widely used affinity tags in biotechnology applications. The present study named a GST gene as the TtGSTz1 gene, characterized with the conserved glutathione binding motif (SSTSWRVRIAL) under a GST zeta subfamily from Tetrahymena thermophila. Phylogenetic analysis of the TtGstz1p protein sequence, with its orthologs from different GST classes, showed that it is a member of the unicellular GSTz monophyletic clade, which is positioned close to the bacterial GSTz clade. Seven codons of the TtGSTz1 gene were first engineered by introducing silent mutations (TAA > CAA or TAG > CAG) to the code for glutamine …
Phytase Production From Lactobacillus Brevis, Meli̇s Sümengen, Sadik Di̇nçer, Ayşenur Kaya
Phytase Production From Lactobacillus Brevis, Meli̇s Sümengen, Sadik Di̇nçer, Ayşenur Kaya
Turkish Journal of Biology
In this study, the phytase from Lactobacillus brevis strains isolated from fermented food products was produced and characterized. Phytase production of this strain was determined by production of clear zones around the colonies on the sodium phytate containing medium, after 48 h of incubation at 30 °C. The molecular weight of the phytase from Lactobacillus brevis was estimated as 73 kDa and 34 kDa on SDS-PAGE. The optimum activity of Lactobacillus brevis extracellular and intracellular enzymes emerged at 120 °C, pH 3.4 and at 110 °C, pH 3.4, respectively. The extracellular enzyme from Lactobacillus brevis was 91% stable for 5 …
Bioremediation Of Endosulfan-Contaminated Soil By Using Bioaugmentation Treatment Of Fungal Inoculant Aspergillus Niger, Tejomyee Sadashiv Bhalerao
Bioremediation Of Endosulfan-Contaminated Soil By Using Bioaugmentation Treatment Of Fungal Inoculant Aspergillus Niger, Tejomyee Sadashiv Bhalerao
Turkish Journal of Biology
The persistence of endosulfan and intermediate metabolite endosulfan sulfate in the environment and their toxic effects on biota necessitate their removal. This study investigated the bioaugmentation of endosulfan-contaminated soil by fungal inoculant Aspergillus niger ARIFCC 1053. The influence of bioaugmentation by A. niger on endosulfan-contaminated soil was evaluated with the help of change in pH and released chloride, and by thin layer chromatography and gas chromatography analysis. Its effects on soil functionality were monitored by estimating dehydrogenase and arylsulfatase enzyme activities. The endosulfan degradation reached an undetectable level on day 15. The pH of the medium was nearly neutral (6.9) …
Antihyperglycemic, Antioxidant And Cytotoxic Activities Of Alocasia Macrorrhizos (Linn.) Rhizomes Extract, Md Masudur Rahman, Md. Aslam Hossain, Saiful Alam Siddique, Kaishar Parvej Biplab, Md. Helal Uddin
Antihyperglycemic, Antioxidant And Cytotoxic Activities Of Alocasia Macrorrhizos (Linn.) Rhizomes Extract, Md Masudur Rahman, Md. Aslam Hossain, Saiful Alam Siddique, Kaishar Parvej Biplab, Md. Helal Uddin
Turkish Journal of Biology
Alocasia macrorrhizos (L.) (Araceae), a coarse and erect plant that is widely cultivated to be eaten as a vegetable throughout Bangladesh, has been reported to possess a number of medicinal properties. The antihyperglycemic, antioxidant, and cytotoxic effects of the methanol extract of A. macrorrhizos rhizomes were investigated. The extract was given at a single dose (250 and 500 mg/kg) in alloxan-induced hyperglycemic mice. The extract at 500 mg/kg produced a significant (P < 0.05) decrease in the blood glucose level (55.49%) at 8 h of treatment when compared with the control and was comparable with the reference drug, metformin. The extract was also subjected to antioxidant potentiality and brine shrimp lethality bioassays. The IC_{50} value of the extract was 693.0 \mug/mL and the LC_{50} was 188.14 \mug/mL.
Genetic Characterization Of Turkish Bread Wheat Landraces Based On Microsatellite Markers And Morphological Characters, Özlem Ateş Sönmezoğlu, Betül Bozmaz, Ahmet Yildirim, Nejdet Kandemi̇r, Nevzat Aydin
Genetic Characterization Of Turkish Bread Wheat Landraces Based On Microsatellite Markers And Morphological Characters, Özlem Ateş Sönmezoğlu, Betül Bozmaz, Ahmet Yildirim, Nejdet Kandemi̇r, Nevzat Aydin
Turkish Journal of Biology
Wheat landraces could be unique sources of favorable genes for agronomic traits like yield and insect, pest, and disease resistances, as well as quality characteristics that are important for breeding programs. Molecular marker systems offer great opportunities to characterize wheat accessions, which in particular are not morphologically identifiable. In this study, 20 bread wheat landraces collected from different regions of Turkey were characterized by using microsatellite markers (SSRs) and morphological characters. Seventeen morphological characters were used. Fifteen SSR primers were prescreened and the 7 most polymorphic primers were employed in characterization. The most polymorphic SSR loci were Xgwm 95 and …
Cardioprotective Effect Of Diazepam On Ischemia-Reperfused Isolated Hyperthyroid Rat Heart, Dareuosh Shackebaei, Atefeh Asadmobini, Mahvash Hesari, Maryam Vaezi, Siamak Shahidi
Cardioprotective Effect Of Diazepam On Ischemia-Reperfused Isolated Hyperthyroid Rat Heart, Dareuosh Shackebaei, Atefeh Asadmobini, Mahvash Hesari, Maryam Vaezi, Siamak Shahidi
Turkish Journal of Biology
Hyperthyroidism increases the vulnerability of the heart to ischemia-reperfusion (I/R) injury. Regarding the fact that diazepam also affects cardiac I/R injury, the current study was conducted to investigate the effect of diazepam on ischemia-reperfused isolated hyperthyroid rat heart. The animals were divided into 6 groups: the control, hyperthyroid, control diazepam (1 mg/kg), hyperthyroid diazepam (1 mg/kg), control diazepam (5 mg/kg), and hyperthyroid diazepam (5 mg/kg) groups. Langendorff-perfused rat hearts underwent 40 min of global ischemia and 45 min of reperfusion. Different cardiac parameters, including the left ventricular developed pressure, heart rate, coronary flow, and rate pressure product (RPP), were measured. …
Effect Of Cell-Conditioned Media On Biomass Production Of Leishmania Parasites, Melahat Bagirova, Rabi̇a Çakir Koç, Adi̇l Allahverdi̇yev, Meli̇ke Ersöz
Effect Of Cell-Conditioned Media On Biomass Production Of Leishmania Parasites, Melahat Bagirova, Rabi̇a Çakir Koç, Adi̇l Allahverdi̇yev, Meli̇ke Ersöz
Turkish Journal of Biology
A large amount of parasite biomass production necessitates a higher volume of culture media, and this further increases the overall cost. For this reason, novel and alternative culture supplementary materials have been continuously investigated. The aim of this study was therefore to evaluate the effect of cell-conditioned media, collected as waste material during routine cell culture, on the growth and development of Leishmania parasites in vitro. In this study, for the first time, we show that cell-conditioned medium obtained from MCF-7 culture increased the growth of Leishmania parasites in vitro. These results show that the metabolites that are present in …
Isolation, Culturing And Characterization Of Rat Adipose Tissue-Derived Mesenchymal Stem Cells: A Simple Technique, Mehmet Ni̇yaz, Özer Ayli̇n Gürpinar, Serdar Günaydin, Mehmet Ali̇ Onur
Isolation, Culturing And Characterization Of Rat Adipose Tissue-Derived Mesenchymal Stem Cells: A Simple Technique, Mehmet Ni̇yaz, Özer Ayli̇n Gürpinar, Serdar Günaydin, Mehmet Ali̇ Onur
Turkish Journal of Biology
In this study, our aim was to develop a new simple technique for isolation of mesenchymal stem cells from adipose tissue. For this purpose, mesenchymal stem cells were isolated from rat adipose tissue by using the primary explant culture technique. When the cells became confluent, they were passaged 4 times by using the standard trypsinization method with trypsin/EDTA solution. Cells at second passage were characterized by using immunofluorescence staining against CD13 and CD29 markers. The results showed that these cultured cells were positive for CD13 and CD29 markers. Flow cytometry analysis was also done against CD29, CD90, CD54, MHC Class …
Assessment Of Antioxidant, Antibacterial, Antimycobacterial, And Antifungal Activities Of Some Plants Used As Folk Remedies In Turkey Against Dermatophytes And Yeast-Like Fungi, Di̇dem Deli̇orman Orhan, Berri̇n Özçeli̇k, Sanem Hoşbaş, Meci̇t Vural
Assessment Of Antioxidant, Antibacterial, Antimycobacterial, And Antifungal Activities Of Some Plants Used As Folk Remedies In Turkey Against Dermatophytes And Yeast-Like Fungi, Di̇dem Deli̇orman Orhan, Berri̇n Özçeli̇k, Sanem Hoşbaş, Meci̇t Vural
Turkish Journal of Biology
Twenty-seven aqueous extracts obtained from 21 plants used in the treatment of respiratory tract infections as folk remedies in Turkey were investigated for their relative total phenolic contents and antioxidant, antibacterial, antimycobacterial, and antifungal activities. Antibacterial and antifungal activities against dermatophytes and yeast-like fungi were determined using the microdilution method. Antibacterial activity of all extracts was more pronounced against gram-positive bacteria than against gram-negative bacteria. These extracts also exhibited higher antifungal activity against Candida krusei than ketaconazole. Six extracts displayed antidermatophytic activity against Epidermophyton floccosum, while only 3 extracts were highly active against Trichophyton rubrum. The extracts showed strong activity …
Purification And Properties Of An Endoglucanase From Aspergillus Niger Vtcc-F021, Thi Hoa Pham, Dinh Thi Quyen, Ngoc Minh Nghiem
Purification And Properties Of An Endoglucanase From Aspergillus Niger Vtcc-F021, Thi Hoa Pham, Dinh Thi Quyen, Ngoc Minh Nghiem
Turkish Journal of Biology
An extracellular endoglucanase (EG) from Aspergillus niger VTCC-F021 was purified 2.09-fold to homogeneity with a yield of 18.4%. The enzyme had a molecular mass of 31 kDa and a specific activity of 14.122 U/mg protein. Optimum temperature was observed at 55 °C and optimum pH at 5. The enzyme was stable up to 50 °C and from pH 5 to 6 with residual activity >80% and 60%, respectively. The kinetic constants K_m and V_{max} determined for EG, with carboxyl methyl cellulose as a substrate, were 8.5815 mg CMC/mL and 20.121 U/mg protein, respectively. EDTA increased EG activity by 35% at …
Evaluation Of Antiinflammatory And Antinociceptive Effects Of Some Juniperus Species Growing In Turkey, Ni̇lüfer Orhan, Esra Akkol, Fatma Ergun
Evaluation Of Antiinflammatory And Antinociceptive Effects Of Some Juniperus Species Growing In Turkey, Ni̇lüfer Orhan, Esra Akkol, Fatma Ergun
Turkish Journal of Biology
Different parts and products of Juniperus species have been used as folk medicine for various infectious diseases, rheumatism, and wounds in Turkey. In order to evaluate this traditional usage, antiinflammatory and antinociceptive activities of leaf and berry extracts of 3 Juniperus species (J. excelsa, J. foetidissima, and J. sabina) were investigated. Additionally, antiinflammatory and antinociceptive activities of subextracts of J. oxycedrus subsp. oxycedrus (Joso) berries and leaves were evaluated. The p-benzoquinone-induced writhing test for antinociceptive activity and the carrageenan-induced hind paw edema model for antiinflammatory activity in mice were employed. Ethanol extract prepared from berries of J. foetidissima provided a …