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Synecological Structure Of The Lichen Synusiae Within Forest Natural Reserves From The Moldavian Plateau (Romania), Ioana Vicol
Synecological Structure Of The Lichen Synusiae Within Forest Natural Reserves From The Moldavian Plateau (Romania), Ioana Vicol
Turkish Journal of Botany
This study describes and assesses the influence of the particular features of microhabitats on the abundances of lichen species. The research was performed in 4 natural reserves: Seaca-Movileni, Badeana, Harboanca-Brahaşoaia, and Balteni (all within Vaslui county). The distribution of lichen species abundances was investigated in 20 cm × 20 cm sampling units at a height of 1 m above the ground. In the studied area 27 lichen species were sampled in 81 sampling units. Cluster analysis indicated both similarities and dissimilarities between the natural reserves investigated. Kruskal-Wallis tests pointed out significant differences regarding tree girths, depth of rhytidome crevices, and …
Cytotoxic Effects Of Various Lactic Acid Bacteria On Caco-2 Cells, Sevda Er, Ayşe Tansu Koparal, Meri̇h Kivanç
Cytotoxic Effects Of Various Lactic Acid Bacteria On Caco-2 Cells, Sevda Er, Ayşe Tansu Koparal, Meri̇h Kivanç
Turkish Journal of Biology
Probiotics are live microbial food supplements that can be considered a functional food. They benefit the health of a host animal by maintaining their intestinal microbial balance. Most probiotic microorganisms are lactic acid bacteria (LAB) such as Lactobacillus spp., Bifidobacterium spp., and Enterococcus spp. LAB have been reported to possess certain anticancer properties. The vast majority of studies on their anticancer effects have dealt with colorectal cancers, although there have also been some studies on breast and bladder cancers. Colon cancer is the fourth most common cause of cancer-related mortality in the world. The aim of this study was to …
Identification And Pathogenicity Of Bacteria In The Mediterranean Corn Borer Sesamia Nonagrioides Lefebvre (Lepidoptera: Noctuidae), Ardahan Eski̇, Fi̇li̇z Özkan Çakici, Mustafa Güllü, Hacer Muratoğlu, Zi̇hni̇ Demi̇rbağ, İsmai̇l Demi̇r
Identification And Pathogenicity Of Bacteria In The Mediterranean Corn Borer Sesamia Nonagrioides Lefebvre (Lepidoptera: Noctuidae), Ardahan Eski̇, Fi̇li̇z Özkan Çakici, Mustafa Güllü, Hacer Muratoğlu, Zi̇hni̇ Demi̇rbağ, İsmai̇l Demi̇r
Turkish Journal of Biology
Sesamia nonagrioides (Lep.: Noctuidae) is one of the most serious pests of corn in Turkey and other Mediterranean countries. Although various cultural, chemical, and biological methods are used to control this pest, its damage still continues in all Mediterranean countries. In this study, to find an effective bacterium that can be used as a biocontrol agent against S. nonagrioides, we isolated 15 bacteria from S. nonagrioides larvae and evaluated the larvicidal potency of all isolates on the pest. According to their morphological, physiological, biochemical, and molecular properties, the isolates were identified as Achromobacter insolitus (Sn1), Morganella morganii (Sn2), Klebsiella pneumoniae …
Genotoxicity Of Metaphase-Arresting Methods In Barley, Aslihan Temel, Nermi̇n Gözükirmizi
Genotoxicity Of Metaphase-Arresting Methods In Barley, Aslihan Temel, Nermi̇n Gözükirmizi
Turkish Journal of Biology
Amiprophos-methyl (APM) and colchicine (COL) are therapeutic agents and are also used in plant science. Cell cycle synchronization (CCS) is mediated by hydroxyurea (HU) and APM. The genotoxic effects of APM are unclear. In order to assess the genotoxicity of APM separately and with HU and then compare it with COL, we treated 2-day-old barley (Hordeum vulgare 'Bornova-92') seedlings with 8 µM APM for 2 h, 1.25 mM COL for 18 h, or 1.25 mM HU for 18 h and 4 µM APM for 2 h, with a final ice-cold water incubation. Following treatment, seedlings were recovered without any substance …
Antioxidant Activity Of Heme Oxygenase 1 In Brassica Juncea (L.) Czern.(Indian Mustard) Under Salt Stress, Khushbu Verma, Shubham Dixit, Gyan Singh Shekhawat, Afroz Alam
Antioxidant Activity Of Heme Oxygenase 1 In Brassica Juncea (L.) Czern.(Indian Mustard) Under Salt Stress, Khushbu Verma, Shubham Dixit, Gyan Singh Shekhawat, Afroz Alam
Turkish Journal of Biology
Antioxidant enzymes play a protective role in plants under oxidative stress. The present study explores the role of antioxidant system heme oxygenase1 (HO1) in Brassica juncea (L.) Czern. in salt stress conditions. B. juncea seedlings were treated with different NaCl concentrations (0-200 mM) for 5 days. Salt stress elicited the highest response at 150 mM NaCl concentration; at this level, gene expression of HO1 was also upregulated. Apart from HO1, ascorbate peroxidase (APX), peroxidase (POD), and glutathione reductase (GR) also showed their highest activity at 150 mM NaCl concentration. The study shows that HO1 is indispensable in B. juncea for …
S. Cerevisiae $\Beta$-Glucan Reduced Viability Of Mouse Hepatoma Cells In Vitro, Artur Javmen, Ausra Nemeikaite-Ceniene, Saulius Grigiskis, Irena Jonauskiene, Mark Rudenkov, Darius Kacianauskas, Mykolas Mauricas
S. Cerevisiae $\Beta$-Glucan Reduced Viability Of Mouse Hepatoma Cells In Vitro, Artur Javmen, Ausra Nemeikaite-Ceniene, Saulius Grigiskis, Irena Jonauskiene, Mark Rudenkov, Darius Kacianauskas, Mykolas Mauricas
Turkish Journal of Biology
$\beta$-Glucan is a natural polymer, which is widely studied due to its multiple immunomodulatory properties. In addition, recent findings indicate potent antitumor properties of $\beta$-glucan. Saccharomyces cerevisiae, baker's yeast, is one of the commonly used sources of $\beta$-1,3-glucan. The aim of this work was to investigate S. cerevisiae $\beta$-glucan immunomodulatory activity against cancer cells.In our experiments, BALB/c mice were fed with insoluble whole $\beta$-glucan particles, and then their blood was collected for experiments. MH-22a hepatoma cells were treated with the blood of mice fed with $\beta$-glucan, and tumor cell viability was investigated after the treatment.The obtained results demonstrated that leukocytes …
Optimization Of Multiplex Rt-Pcr For M1, M23, And M23x Splice Variants Of Aqp4 And Ss-Actin Transcripts In Dalton?S Lymphoma Mouse Tissues, Rajaneesh Kumar Gupta, Sukala Prasad
Optimization Of Multiplex Rt-Pcr For M1, M23, And M23x Splice Variants Of Aqp4 And Ss-Actin Transcripts In Dalton?S Lymphoma Mouse Tissues, Rajaneesh Kumar Gupta, Sukala Prasad
Turkish Journal of Biology
Multiplex reverse transcriptase-polymerase chain reaction (MRT-PCR) is a method of choice for efficient and simultaneous analysis of gene expression at the transcript level with limited amounts of tissues; however, it is less common than other methods due to certain limitations. Here, we describe the protocol for MRT-PCR-based semiquantitative analysis of all AQP4 transcripts simultaneously in Dalton's lymphoma (DL) using transcript-specific primers and outline the critical parameters. Aquaporin-4 (AQP4) water channels are associated with edema of tissues due to various diseases. The AQP4 transcript possesses alternative transcription initiation sites and produces three isoforms, AQP4.M1, AQP4.M23, and AQP4.M23X, which constitute heterotetrameric orthogonal …
Use Of In Vitro Propagation Of `Obla?Inska? Sour Cherry In Rootstock Breeding, Dusica Doric, Vladislav Ognjanov, Goran Barac, Mirjana Ljubojevic, Ankica Pranjic, Krunoslav Dugalic, Sezai̇ Erci̇şli̇
Use Of In Vitro Propagation Of `Obla?Inska? Sour Cherry In Rootstock Breeding, Dusica Doric, Vladislav Ognjanov, Goran Barac, Mirjana Ljubojevic, Ankica Pranjic, Krunoslav Dugalic, Sezai̇ Erci̇şli̇
Turkish Journal of Biology
Prunus cerasus L. `Obla?inska? sour cherry germplasm was established in vitrodirectly from in situ plants on different explant collection dates, enabling quick clonal multiplication and introduction to a rootstock breeding program. Rosette initiation of four investigated genotypes was possible from November to April on the medium containing Schenk and Hildebrandt (SH) macroelements, Murashige and Skoog (MS) microelements, and vitamins supplemented with (in mg L?1) 6-benzyladenine (BA), 0.5; indole-3-butyric acid (IBA), 0.01; gibberellic acid (GA3), 0.1; citric acid, 10; and L-ascorbic acid, 10. The lowest percent of contamination was noted in November and December, when dormant buds were used as an …
Storage Temperature Of Boar Semen And Its Relationship To Changes In Sperm Plasma Membrane Integrity, Mitochondrial Membrane Potential, And Oxidoreductive Capability, Dariusz Gaczarzewicz, Jan Udala, Malgorzata Piasecka, Barbara Blaszczyk, Tomasz Stankiewicz
Storage Temperature Of Boar Semen And Its Relationship To Changes In Sperm Plasma Membrane Integrity, Mitochondrial Membrane Potential, And Oxidoreductive Capability, Dariusz Gaczarzewicz, Jan Udala, Malgorzata Piasecka, Barbara Blaszczyk, Tomasz Stankiewicz
Turkish Journal of Biology
The aim of this study was to analyze changes in sperm plasma membrane integrity, mitochondrial activity, and extracellular environment during storage of boar semen at 5 °C, 16 °C, and 25 °C for 10 days. Progressive sperm motility, plasma membrane integrity (SYBR-14/PI-test and HOS test), aspartate aminotransferase (AAT), and mitochondrial activity (JC-1-test and NADH-dependent NBT assay), as well as pH and osmolality were assessed in nine ejaculates of Polish Landrace boars. The plasma membrane integrity of the semen stored at 5 °C was similar to that of the semen stored at 16 °C and 25 °C; however, an increase in …
Role Of Melatonin On Calcium Signaling And Mitochondrial Oxidativestress In Epilepsy: Focus On Trp Channels, Mustafa Naziroğlu
Role Of Melatonin On Calcium Signaling And Mitochondrial Oxidativestress In Epilepsy: Focus On Trp Channels, Mustafa Naziroğlu
Turkish Journal of Biology
Calcium ion (Ca2+) accumulation and excessive oxidative stress in the hippocampus and brain cortex have long been known as major contributors to the etiology of epilepsy. I have reviewed the role of Ca2+ signaling through cation channels and mitochondria-mediated oxidative stress on epilepsy in human and animals. A review of the relevant papers and results from recent studies were obtained from PubMed and the Science Citation Index. Current literature findings indicate that melatonin and agomelatine reduce activation of hippocampal transient receptor potential (TRP), glutamate receptors, and voltage-gated calcium channels that are critical for the development of abnormal Ca2+ homeostasis and …
Melatonin As A Stabilizer Of Mitochondrial Function: Role In Diseases And Aging, Cristina Carrasco, Ana B. Rodriguez, Jose A. Pariente
Melatonin As A Stabilizer Of Mitochondrial Function: Role In Diseases And Aging, Cristina Carrasco, Ana B. Rodriguez, Jose A. Pariente
Turkish Journal of Biology
It is now almost 60 years since the discovery of melatonin and new physiological functions of the indole continuously appear in the most recent studies worldwide. Experimental evidence emphasizes its importance as a stabilizer of the mitochondrial bioenergetics, which could be related to the prevention of development of aging and several diseases. In the next years, conscientious investigation about this topic should be undertaken by scientists of different research areas to achieve a better understanding of the molecular mechanisms implied. This will ultimately allow the development and clinical application of efficacious treatments.
Prooxidant Effects Of Melatonin: A Brief Review, Malwina S. Munik, Cem Ekmekçi̇oğlu
Prooxidant Effects Of Melatonin: A Brief Review, Malwina S. Munik, Cem Ekmekçi̇oğlu
Turkish Journal of Biology
Melatonin acts classically through the widely expressed G protein-coupled membrane receptors MT1 and MT2, respectively. The functional role of the MT receptors is not fully clear with multiple effects, such as effects on the circadian, reproductive, immune, cardiovascular, and intestinal systems, being suggested. In addition to receptor-mediated effects, melatonin also acts as a potent antioxidant and it is quite evident that melatonin and its metabolites efficiently reduce oxidative damage to proteins, lipids, and DNA and also exert protective effects on mitochondria. The potent antioxidant activity of melatonin stems from various complex mechanisms, including direct scavenging of radicals, stimulation of antioxidant …
Amelioration Of Subchronic Acrylamide Toxicity In Large Intestine Of Rats Byorganic Dried Apricot Intake, Mehmet Erman Erdemli̇, Zümrüt Doğan, Yilmaz Çi̇ğremi̇ş, Müslüm Akgöz, Eyüp Altinöz, Murat Geçer, Yusuf Türköz
Amelioration Of Subchronic Acrylamide Toxicity In Large Intestine Of Rats Byorganic Dried Apricot Intake, Mehmet Erman Erdemli̇, Zümrüt Doğan, Yilmaz Çi̇ğremi̇ş, Müslüm Akgöz, Eyüp Altinöz, Murat Geçer, Yusuf Türköz
Turkish Journal of Biology
Acrylamide (AA) has neurotoxic, mutagenic, and genotoxic effects in humans and experimental animals. Fruit consumption is important for human health, because fruits are the source of many nutrients such as vitamins, minerals, carotenoids, dietary fiber, and phytonutrients. Many agricultural products provide natural melatonin in the diet. At the onset of the study, rats were weighted and randomly divided into four groups each containing 10 rats as follows: group 1: control (fed with normal diet and normal drinking water); group 2: apricot (fed with a daily diet with 5% apricot and normal drinking water); group 3: AA (administered daily acrylamide at …
The Effects Of Agomelatine And Melatonin On Ecog Activity Of Absenceepilepsy Model In Wag/Rij Rats, Hati̇ce Aygün, Duygu Aydin, Sema İnanir, Fati̇h Eki̇ci̇, Mustafa Ayyildiz, Erdal Ağar
The Effects Of Agomelatine And Melatonin On Ecog Activity Of Absenceepilepsy Model In Wag/Rij Rats, Hati̇ce Aygün, Duygu Aydin, Sema İnanir, Fati̇h Eki̇ci̇, Mustafa Ayyildiz, Erdal Ağar
Turkish Journal of Biology
The aim of this study was to evaluate the effect of the melatonergic M1 and M2 receptor agonist and serotonergic 5-HT2C receptor antagonist agomelatine on the spike wave discharges (SWDs) seen in electrocorticographic (ECoG) recordings of WAG/Rij rats with absence epilepsy. Twenty-one WAG/Rij male rats were used in this study. Tripolar electrodes were placed on skulls and control ECoG activities were recorded. Experimental groups received normal saline (Group I: 1 mL, intraperitoneally (i.p)), agomelatine (Group II: 40 mg/kg, i.p), and melatonin (Group III: 40 mg/kg, i.p) injections for 7 days. Following this period, 2-h ECoG recordings were repeated. The number …
Melatonin Is A Potent Modulator Of Antioxidative Defense And Cellular Proliferation Against Aluminum Toxicity In Rats, Ni̇hal Ömür Bulan, Guner Sarikaya-Unal, Sevim Tunali, Pelin Arda Pi̇ri̇nççi̇, Refiye Yanardag
Melatonin Is A Potent Modulator Of Antioxidative Defense And Cellular Proliferation Against Aluminum Toxicity In Rats, Ni̇hal Ömür Bulan, Guner Sarikaya-Unal, Sevim Tunali, Pelin Arda Pi̇ri̇nççi̇, Refiye Yanardag
Turkish Journal of Biology
Aluminum is the most abundant metal on earth, known for its toxicity to humans and laboratory animals. Melatonin, produced by the pineal gland, has been shown to be an effective antioxidant. In this study, we investigated the possible protective role of melatonin in modulating toxicity and oxidative stress induced by chronic exposure to aluminum in the small intestine of rats. Wistar albino rats were divided into five groups. Group I served as the control and received only physiological saline, Group II served as the control for the melatonin group and received ethanol and physiological saline, Group III received melatonin, Group …
Melatonin Increased Vitamin C And Antioxidant Enzyme Values In The Plasma,Heart, Liver, And Kidney Of Adriamycin-Treated Rats, Ali̇ Zi̇ya Karakilçik, Muharrem Bi̇ti̇ren, Mustafa Zeri̇n, Haki̇m Çeli̇k, Nurten Aksoy
Melatonin Increased Vitamin C And Antioxidant Enzyme Values In The Plasma,Heart, Liver, And Kidney Of Adriamycin-Treated Rats, Ali̇ Zi̇ya Karakilçik, Muharrem Bi̇ti̇ren, Mustafa Zeri̇n, Haki̇m Çeli̇k, Nurten Aksoy
Turkish Journal of Biology
Adriamycin, an anticarcinogenic agent, causes excessive production of reactive oxygen species (ROS). These radicals may affect antioxidant defense systems. The present study was designed to investigate the effects of melatonin on antioxidant enzyme activities and vitamin C in Adriamycin-treated rats. In the current study, rats were divided into three groups: control, Adriamycin, and melatonin+Adriamycin groups. The control group was intraperitoneally injected physiological saline (0.9%) as a placebo. The Adriamycin and melatonin+Adriamycin groups were administered Adriamycin (25 mg/kg body weight). The melatonin+Adriamycin group was pretreated with melatonin (0.5 mg/kg body weight). Vitamin C concentration and catalase (CAT), superoxide dismutase (SOD), glutathione …
Dna Protective And Antioxidative Effects Of Melatonin In Streptozotocin-Induced Diabetic Rats, Seli̇m Sekki̇n, Eda Duygu İpek, Murat Boyacioğlu, Cavi̇t Kum, Ümi̇t Karademi̇r, Hande Sultan Yalinkilinç, Mehmet Onur Ak, Hulki̇ Başaloğlu
Dna Protective And Antioxidative Effects Of Melatonin In Streptozotocin-Induced Diabetic Rats, Seli̇m Sekki̇n, Eda Duygu İpek, Murat Boyacioğlu, Cavi̇t Kum, Ümi̇t Karademi̇r, Hande Sultan Yalinkilinç, Mehmet Onur Ak, Hulki̇ Başaloğlu
Turkish Journal of Biology
Diabetes mellitus is a chronic disease characterized by elevated blood sugar levels. In diabetic patients, oxidative stress induced by the presence of excessive free radicals is closely associated with chronic in?ammation, leading to potential tissue damage. Melatonin (MEL) is a compound synthesized by the pineal gland and a scavenger of free radicals. The aim of this study was to research the effects of MEL on oxidative stress and its DNA protective effects in streptozotocin-induced diabetic rats. In total, 32 rats were equally divided into four experimental groups: control, melatonin, diabetic, and diabetic + melatonin. A single dose of streptozotocin (60 …
Effects Of Photoperiod Variations And Alpha-Lipoic Acid Treatment On Melatonin,Cortisol, And Oxidative Stress Levels In The Blood Of Rats, Cahi̇de Çevi̇k, Recep Aslan
Effects Of Photoperiod Variations And Alpha-Lipoic Acid Treatment On Melatonin,Cortisol, And Oxidative Stress Levels In The Blood Of Rats, Cahi̇de Çevi̇k, Recep Aslan
Turkish Journal of Biology
This study aimed to investigate the effects of frequently changing photoperiods and alpha-lipoic acid treatment on melatonin, cortisol, oxidative markers, and some blood parameters. Eighty-four rats were randomly and equally divided into 7 groups: a control group, 3 prolonged light groups, and 3 prolonged dark groups. The study was completed in 2 stages, with 1- and 4-week periods, respectively. Blood samples were obtained at the end of the first and fourth weeks, and at the end of the dark period for the first and second stages. Serum aspartate aminotransferase and creatinine levels were significantly elevated in the prolonged dark olive …
A New Record For The Spider Fauna Of Iran: Olios Sericeus (Araneae: Sparassidae), Salman Shalchian Tabrizi, Shahrokh Pashaei Rad, Zeinab Hedayati
A New Record For The Spider Fauna Of Iran: Olios Sericeus (Araneae: Sparassidae), Salman Shalchian Tabrizi, Shahrokh Pashaei Rad, Zeinab Hedayati
Turkish Journal of Zoology
In this study, Olios sericeus (Kroneberg, 1875) is introduced as a new record to the fauna of the spiders of Iran. Photos and a drawing of the male specimen are presented and its zoogeographic distribution is discussed.
Alopecosa Psammophila Buchar, 2001 (Araneae: Lycosidae): Morphometricdata And First Record For Romania, Eva Hajnalka Sas Kovacs, Istvan Sas Kovacs, Istvan Urak
Alopecosa Psammophila Buchar, 2001 (Araneae: Lycosidae): Morphometricdata And First Record For Romania, Eva Hajnalka Sas Kovacs, Istvan Sas Kovacs, Istvan Urak
Turkish Journal of Zoology
The present work reports for the first time the occurrence of Alopecosa psammophila (Araneae: Lycosidae) in Romania. It was identified in the autumn of 2013 in 2 habitats located in northwestern Romania, at a Natura 2000 site. Both habitats are represented by open and dry sandy grasslands. Comparison of standard body measurements showed that males have longer legs than females, but the total body length of females is greater than that of males.
A New And Simple Technique For The Isolation Of Symbiotic Bacteria Associated With Entomopathogenic Nematodes (Heterorhabditidae And Steinernematidae), Derya Uluğ, Canan Hazir, Selçuk Hazir
A New And Simple Technique For The Isolation Of Symbiotic Bacteria Associated With Entomopathogenic Nematodes (Heterorhabditidae And Steinernematidae), Derya Uluğ, Canan Hazir, Selçuk Hazir
Turkish Journal of Zoology
This study describes a new, simple, and economical method for isolation of symbiotic bacteria from entomopathogenic nematodes. Isolation of the symbiotic bacteria from entomopathogenic nematodes is useful for mass production of the nematodes. In addition to traditional isolation methods from infected insect larvae or infective juveniles, we show a new method of isolating symbiotic bacteria from either gravid females or females in the endotokia matricida stage, producing symbiont colonies after a very short period of surface sterilization.
Plant Functional Types In Mediterranean Enclaves In Western Black Sea Region Of Turkey, Emi̇re Elmas, Hamdi̇ Güray Kutbay
Plant Functional Types In Mediterranean Enclaves In Western Black Sea Region Of Turkey, Emi̇re Elmas, Hamdi̇ Güray Kutbay
Turkish Journal of Botany
Mediterranean-type communities of the Sinop Peninsula were classified as plant functional types (PFTs) by the help of some vegetative and generative traits and 5 PFTs were determined. Some vegetative (specific leaf area (SLA), leaf texture, leaf P concentration, deciduousness, hairiness) and generative (reproductive phenology and fruit type) traits were found to be significant for discrimination of PFTs. It was found that PFT3 and PFT4 were associated with leaf texture, SLA, and leaf P concentration. PFT2 was associated with deciduousness, hairiness, and reproductive phenology, while PFT1 was only associated with fruit type. Generative traits were found to be associated with PFT1 …