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Adsorption Of Cellulase On Poly(2-Hydroxyethyl Methacrylate) Cryogels Containing Phenylalanine, Murat Yavuz, Oğuz Çakir, Zübeyde Baysal
Adsorption Of Cellulase On Poly(2-Hydroxyethyl Methacrylate) Cryogels Containing Phenylalanine, Murat Yavuz, Oğuz Çakir, Zübeyde Baysal
Turkish Journal of Chemistry
The aim of this study was to prepare a supermacroporous cryogel that can be used for the adsorption of cellulase. The macroporous cryogel of poly(2-hydroxyethyl methacrylate-N-methacryloyl-l-phenylalanine) [p(HEMA-MAPA)] was prepared by copolymerization of 2-hydroxyethyl methacrylate (HEMA) with a functional monomer of N-methacryloyl-l-phenylalanine (MAPA). The cryogel was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and swelling tests. The effects of several parameters such as medium pH, temperature, ionic strength, and flow rate on cellulase adsorption were also investigated. Maximum cellulase adsorption was observed at 25 $^{\circ}$C and pH 4.0. Furthermore, adsorbed cellulase was desorbed from the cryogel by using 1.0 …
Chromium Redox Speciation In Food Samples, Krystyna Pyrzynska
Chromium Redox Speciation In Food Samples, Krystyna Pyrzynska
Turkish Journal of Chemistry
Chromium is an element with important biological characteristics, depending on its different species. Cr(III) is considered as essential; however, the Cr(VI) form is classified as carcinogenic.~For this reason, speciation analysis in food samples is a very important question. The proposed data procedures for chromium redox speciation in the literature can be divided into those with the use of online hyphenated techniques and those where appropriate sample pretreatment is necessary. The strategies for nonchromatographic speciation are mainly based on selective liquid--liquid extraction procedures, coprecipitation, selective separation of chromium species using a solid phase extraction column, or complexation reactions. In this paper, …
Synthesis And Structural Properties Of $N$-3,4-(Dichlorophenyl)-3-Oxo-3- Phenyl-2-(Phenylcarbonyl)Propanamide And Its Cu(Ii) Complex, Ahmet Oral Sarioğlu, Tuğba Taşkin Tok, Mehmet Akkurt, Muhammad Nawaz Tahir, Mehmet Sönmez
Synthesis And Structural Properties Of $N$-3,4-(Dichlorophenyl)-3-Oxo-3- Phenyl-2-(Phenylcarbonyl)Propanamide And Its Cu(Ii) Complex, Ahmet Oral Sarioğlu, Tuğba Taşkin Tok, Mehmet Akkurt, Muhammad Nawaz Tahir, Mehmet Sönmez
Turkish Journal of Chemistry
A new N-carboxamide compound (3) was synthesized by the reaction of dibenzoylaceticacid-$N$-carboxyethyla\-mide (1) and 3,4-dichloroaniline (2). The $N$-(3,4-dichlorophenyl)-3-oxo-3-phenyl-2-(phenylcarbonyl) propanamide (3) subsequently reacted with Cu salt to produce its Cu(II) complex compound (4). The compounds were characterized by analytical and spectral methods. In addition, X-ray diffraction was performed to characterize and obtain detailed information about the structure of 3. The fully optimized geometries of compounds 3 and 4 were calculated at different basis sets by using the Gaussian09 (G09) software to investigate their 3D geometries and electronic structures. Comparisons between the calculated and experimental data including molecular structures, fundamental vibrational modes, …
Investigation Of Long-Term Water Absorption Behavior Of Carbon Fabric Reinforced Epoxy Composites Containing Hydrophobic Nanosilica, Nurten Eki̇n, Mehmet Gali̇p İçduygu, Ahmet Gültek
Investigation Of Long-Term Water Absorption Behavior Of Carbon Fabric Reinforced Epoxy Composites Containing Hydrophobic Nanosilica, Nurten Eki̇n, Mehmet Gali̇p İçduygu, Ahmet Gültek
Turkish Journal of Chemistry
hydrophobic filler containing epoxy matrix composites was investigated by keeping them in distilled water for 9 months. Aerosil R812 (8 nm) and Aerosil R972 (12 nm) were utilized at 3{\%} and 5{\%} w/w filler to resin ratio and the resin/filler mixture was combined with 12-ply carbon fabric to get 15 $\times $ 15 cm samples. Samples were molded under a hot press and for each sample 50 test samples obtained by cutting them with a water jet. The obtained samples were characterized by TG, FTIR, and SEM. The effect of water absorption was evaluated by measuring percentage water absorption and …
Mn-Doped Zns Quantum Dots As A Room-Temperature Phosphorescent Probe For Analysis Of Glutamic Acid In Foodstuffs, Hayri̇ye Eda Şatana Kara, Burak Demi̇rhan, Buket Er Demi̇rhan
Mn-Doped Zns Quantum Dots As A Room-Temperature Phosphorescent Probe For Analysis Of Glutamic Acid In Foodstuffs, Hayri̇ye Eda Şatana Kara, Burak Demi̇rhan, Buket Er Demi̇rhan
Turkish Journal of Chemistry
L-cysteine-capped Mn-doped ZnS quantum dots (QDs) were used for the determination of glutamic acid in foodstuffs. This method is based on measurement of the quenching of the phosphorescence intensity of the QDs after interacting with glutamic acid. A linear response was observed from 50 to 500 ng mL$^{-1}$ glutamic acid with a limit of detection of 6.79 ng mL$^{-1}$. Room temperature phosphorescence (RTP) intensity of the QDs was quenched rapidly upon the addition of the quencher and the reaction reached equilibrium within 2 min. The quenching mechanism of phosphorescence of Mn-doped ZnS QDs by glutamic acid is dynamic and the …
Production, Purification, And Characterization Of A Thermo-Alkali Stable And Metal-Tolerant Carboxymethylcellulase From Newly Isolated Bacillus Methylotrophicus Y37, Yonca Duman, Yonca Yüzügüllü Karakuş, Arzu Sertel, Fi̇kri̇ye Polat
Production, Purification, And Characterization Of A Thermo-Alkali Stable And Metal-Tolerant Carboxymethylcellulase From Newly Isolated Bacillus Methylotrophicus Y37, Yonca Duman, Yonca Yüzügüllü Karakuş, Arzu Sertel, Fi̇kri̇ye Polat
Turkish Journal of Chemistry
A carboxymethylcellulose (CMC)-degrading bacterium was isolated from soil, identified as Bacillus methylotrophicus according to the physiological properties and analyses of 16S rRNA and a partial sequence of the gyrase A (gyrA) gene, and named as B. methylotrophicus Y37. The CMCase enzyme was purified to homogeneity by 20.4-fold with 21.73% recovery using single-step hydrophobic interaction chromatography and biochemically characterized. CMCase showed a molecular weight of approximately 50 kDa as determined by SDS-PAGE. The activity profile of the CMCase enzyme exhibited optimum activity at 45 $^{\circ}$C and pH 5.0. The activity was highly stable at alkaline pH levels. More than 90% of …
Comparative Study For The Separation, Preconcentration, And Determination Of Copper And Cadmium In Real Samples By Using Two Different Ligands, Hami̇de Elvan Bayrak, Volkan Numan Bulut, Mehmet Tüfekçi̇, Hacer Bayrak, Celal Duran, Mustafa Soylak
Comparative Study For The Separation, Preconcentration, And Determination Of Copper And Cadmium In Real Samples By Using Two Different Ligands, Hami̇de Elvan Bayrak, Volkan Numan Bulut, Mehmet Tüfekçi̇, Hacer Bayrak, Celal Duran, Mustafa Soylak
Turkish Journal of Chemistry
Two new solid phase extraction methods have been developed for simultaneous separation and preconcentration of Cu(II) and Cd(II) in some food and environmental samples before their determination by flame atomic absorption spectrometry (FAAS). One of these methods is based on the absorption of the Cu(II) and Cd(II) complexes with [3-(cyanomethyl)-5-(4-methylphenyl)-4$H$-1,2,4-triazol-4-yl]carbamate (L1) and the other is based on the absorption of the Cu(II) and Cd(II) complexes with 4-(4-methylphenyl)-5-{[pyridine-4-yl-4$H$-1,2,4-triazole-3-yl)thio]methyl}-4$H$-1,2,4-triazole-3-thiol (L2) on Amberlite XAD-1180 resin. The metal complexes are retained on resin at pH 6.0 and eluted with 1.0 mol L$^{-1}$ HCl in acetone in both methods. After the optimum conditions were determined, …
Synthetic Protocols On 6$H$-Benzo[$C$]Chromen-6-Ones: A Review, Ofentse Mazimba
Synthetic Protocols On 6$H$-Benzo[$C$]Chromen-6-Ones: A Review, Ofentse Mazimba
Turkish Journal of Chemistry
6$H$-Benzo[$c$]chromen-6-ones serve as core structures of secondary metabolites and are of considerable pharmacological importance. Natural sources produce limited quantities, hence the need for synthetic procedures for 6$H$-benzo[$c$]chromen-6-ones, which are herein reviewed. The literature describes protocols such as the Suzuki coupling reactions for the synthesis of biaryl, which then undergoes lactonization, reactions of 3-formylcoumarin (chromenones) with 1,3-bis(silylenol ethers), radical mediated cyclization of arylbenzoates, metal or base catalyzed cyclization of phenyl-2-halobenzoates and 2-halobenzyloxyphenols, and benzoic acid coupling with benzoquinone using electrophilic metal-based catalyst. The efficient and simple procedures are those involving the reactions of Michael acceptor (chromenones and chalcones) with 1,3- and …
Immunohistochemical Identification Of Aquaporin 2 In The Kidneys Of Wild Boars (Sus Scrofa), Katarzyna Michalek, Ewa Czerniawska-Piatkowska, Marta Grabowska, Maria Laszczynska
Immunohistochemical Identification Of Aquaporin 2 In The Kidneys Of Wild Boars (Sus Scrofa), Katarzyna Michalek, Ewa Czerniawska-Piatkowska, Marta Grabowska, Maria Laszczynska
Turkish Journal of Biology
No abstract provided.
Eukaryotic Expression, Purification, Identification, And Tissuedistribution Of Porcine Pid1, Xiaoling Chen, Huan Wang, Bo Zhou, Zhiqing Huang, Gang Jia, Guangmang Liu, Hua Zhao
Eukaryotic Expression, Purification, Identification, And Tissuedistribution Of Porcine Pid1, Xiaoling Chen, Huan Wang, Bo Zhou, Zhiqing Huang, Gang Jia, Guangmang Liu, Hua Zhao
Turkish Journal of Biology
No abstract provided.
Effects Of A Western-Style Diet High In Cholesterol And Saturated Faton The Rabbit Exocrine Pancreas, María Alba Martínez Burgos, Maria Dolores Yago Torregrosa, Maria Dolores Mesa García, Maria Belen López Millán, Jesús Alcalá Bejarano Carrillo, Ana Rivas Velasco, Ángel Gil Hernández, Mariano Mañas Almendros, Emilio Martínez De Victoria Muñoz
Effects Of A Western-Style Diet High In Cholesterol And Saturated Faton The Rabbit Exocrine Pancreas, María Alba Martínez Burgos, Maria Dolores Yago Torregrosa, Maria Dolores Mesa García, Maria Belen López Millán, Jesús Alcalá Bejarano Carrillo, Ana Rivas Velasco, Ángel Gil Hernández, Mariano Mañas Almendros, Emilio Martínez De Victoria Muñoz
Turkish Journal of Biology
No abstract provided.
Development Of Therapeutic Proteins: Advances And Challenges, Muhammad Sajid Hamid Akash, Kanwal Rehman, Muhammad Tariq, Shuqing Chen
Development Of Therapeutic Proteins: Advances And Challenges, Muhammad Sajid Hamid Akash, Kanwal Rehman, Muhammad Tariq, Shuqing Chen
Turkish Journal of Biology
Therapeutic proteins have shown to be effective against a variety of diseases. Pharmaceutical companies are progressively focusing on research using proteins in search of novel effective therapeutics. The significant attention by researchers has resulted in considerable advances in the production and usage of therapeutic proteins in recent years. In the current study we focused on the understanding of therapeutic proteins and their impact on the human health care system. Advancements in the field of biotechnology have increased and facilitated the production of therapeutically significant proteins to combat various fatal diseases. Although protein-based therapeutics have taken center stage in drug discovery …
Changes In Gene Expression In Sirt3 Knockout Liver Cells, Randa Tao, Jamie Leclerc, Kübra Yildiz, Seong-Hoon Park, Barbara Jung, David Gius, Özkan Özden
Changes In Gene Expression In Sirt3 Knockout Liver Cells, Randa Tao, Jamie Leclerc, Kübra Yildiz, Seong-Hoon Park, Barbara Jung, David Gius, Özkan Özden
Turkish Journal of Biology
The sirtuin (SIRT) gene family is reported to regulate critical intracellular processes from aging to cellular metabolism and repair. SIRT3 knockout (SIRT3-/-) mice develop receptor positive mammary tumors starting at 13 months and SIRT3 expression is decreased in human breast cancer samples as well as several other diseases. It is established that carcinogenesis results from the accumulation of multiple aberrant genetic events including the activation of oncogenes and inactivation of tumor-suppressor genes. To determine the potential early genomic events that may play a role in the tumor-permissive phenotype observed in SIRT3-/- cells, we compared gene expression profile in SIRT3-/- and …
Immobilized Periphytic Cyanobacteria For Removal Of Nitrogenous Compounds And Phosphorus From Shrimp Farm Wastewater, Sanjoy Banerjee, Helena Khatoon, Mohamed Shariff, Fatimah Yusoff
Immobilized Periphytic Cyanobacteria For Removal Of Nitrogenous Compounds And Phosphorus From Shrimp Farm Wastewater, Sanjoy Banerjee, Helena Khatoon, Mohamed Shariff, Fatimah Yusoff
Turkish Journal of Biology
Cyanobacteria can be used to remove nitrogenous compounds from wastewater, but a major bottleneck in the process is the separation of cyanobacterial biomass from the treated water discharge, which may cause eutrophication. The current study assessed the suitability of three periphytic cyanobacteria (Geitlerinema sp., Gloeotrichia sp., and Lyngbya sp.) isolated from shrimp ponds. These cyanobacteria were immobilized by self-adhesion to polyvinyl chloride sheets, forming mats, and were screened for their efficacy to reduce nitrogenous compounds and phosphorus. Among the three isolates, Geitlerinema sp. showed the highest reduction rate (98%) and was further tested using shrimp pond wastewater. The results showed …
Antibacterial And Cytotoxic Properties Of Boron-Containing Dental Composite, Selami̇ Demi̇rci̇, Mustafa Sarp Kaya, Ayşegül Doğan, Şaban Kalay, Nergi̇s Özlem Altin, Ayşen Yarat, Serap Hati̇ce Akyüz, Fi̇kretti̇n Şahi̇n
Antibacterial And Cytotoxic Properties Of Boron-Containing Dental Composite, Selami̇ Demi̇rci̇, Mustafa Sarp Kaya, Ayşegül Doğan, Şaban Kalay, Nergi̇s Özlem Altin, Ayşen Yarat, Serap Hati̇ce Akyüz, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
Secondary dental caries are one of the major reasons for restoration replacements. Incorporating antimicrobial properties into dental materials would limit the initiation and progression of dental caries. In the current study, dental composites having 1%, 5%, and 10% (w/w) sodium pentaborate pentahydrate were prepared and analyzed for their mechanical properties, degree of monomer conversion (DC) rate, antibacterial effects against Streptococcus mutans, and biocompatibility with human dental pulp stem cells (hDPSCs). Incorporation of boron into the composites significantly decreased flexural strength and DC in a dose-dependent manner, but the value for 1% boron-containing composite still remained within acceptable levels. Compressive strength …
Whole-Genome Dna Methylation Analysis In Cotton (Gossypium Hirsutum L.) Under Different Salt Stresses, Xuke Lu, Xiaojie Zhao, Delong Wang, Zujun Yin, Junjuan Wang, Weili Fan, Shuai Wang, Tianbao Zhang, Wuwei Ye
Whole-Genome Dna Methylation Analysis In Cotton (Gossypium Hirsutum L.) Under Different Salt Stresses, Xuke Lu, Xiaojie Zhao, Delong Wang, Zujun Yin, Junjuan Wang, Weili Fan, Shuai Wang, Tianbao Zhang, Wuwei Ye
Turkish Journal of Biology
No abstract provided.
Diazoxide Attenuates Ros Generation And Exerts Cytoprotection Under Conditions Of Ros Overproduction In Rat Uterus Cells, Olga Vadzyuk, Sergiy Kosterin
Diazoxide Attenuates Ros Generation And Exerts Cytoprotection Under Conditions Of Ros Overproduction In Rat Uterus Cells, Olga Vadzyuk, Sergiy Kosterin
Turkish Journal of Biology
Reactive oxygen species (ROS) overproduction may severely affect cell functions and even provoke cell death. Diazoxide, an opener of ATP-sensitive potassium channels, induces pharmacological preconditioning in different types of cells. However, the target of action of diazoxide is not clear enough because this substance can activate ATP-sensitive potassium channels localized in mitochondria (mitoKATP) as well as in plasma membranes (KATP channels). It has not yet been established if diazoxide activates mitoKATP in uterus cells. Our objective was to examine the influence of diazoxide on ROS production and on the viability of rat uterus cells under oxidative stress conditions. Using an …
Ultrastructural Effects Of Lead Acetate On The Spleen Of Rats, Mesure Türkay, Hüseyi̇n Türker, Turan Güven
Ultrastructural Effects Of Lead Acetate On The Spleen Of Rats, Mesure Türkay, Hüseyi̇n Türker, Turan Güven
Turkish Journal of Biology
In this experiment, the ultrastructural effects of orally administered lead acetate on rat (Rattus rattus) spleen were studied. Rats weighing approximately 200-250 g were used in this experiment. They were divided into five groups, each one including five rats. Group I served as the normal control group while the others served as the experimental groups. The rats were exposed to oral administrations of lead acetate of 2.5 mg dissolved in 1 mL of drinking water per day. The administration lasted 96, 144, 336, and 432 h. The animals were anesthetized using chloroform inhalation and the peritoneum was stripped open and …
Purification And Characterization Of A Cyanide-Degrading Nitrilase Fromtrichoderma Harzianum Vsl291, Jorge Ricaño-Rodriguez, Mario Ramirez Lepe
Purification And Characterization Of A Cyanide-Degrading Nitrilase Fromtrichoderma Harzianum Vsl291, Jorge Ricaño-Rodriguez, Mario Ramirez Lepe
Turkish Journal of Biology
An intracellular nitrilase (Nit1) with cyanide-degrading activity was isolated from Trichoderma harzianum VSL291, cultivated on benzonitrile as the sole carbon source. Nit1 was purified to homogeneity by ion exchange and gel filtration chromatography with a recovery of 7.15% and a fold of 22.5. The molecular weight was estimated to be 47.7 kDa and the purified enzyme was sequenced with a system of liquid chromatography and mass spectrometry (LC-MS). The enzyme consists of 436 amino acids with a predicted molecular weight of 47.088 kDa. The sequence revealed conserved domains for a nitrilase super family such as putative active and binding sites …
Investigation Of Mode Of Action Of Dna Insecticides On Thebasis Of Ldmnpv Iap-3 Gene, Volodymyr V. Oberemok, Palmah M. Nyadar
Investigation Of Mode Of Action Of Dna Insecticides On Thebasis Of Ldmnpv Iap-3 Gene, Volodymyr V. Oberemok, Palmah M. Nyadar
Turkish Journal of Biology
This study investigates the mechanism of action of DNA insecticides, a novel preparation against gypsy moth (Lymantria dispar) based on the DNA fragments of the antiapoptotic gene of its nuclear polyhedrosis virus. In our experiments gypsy moth showed significant susceptibility to applied DNA insecticides on the basis of BIR and RING domains fragments of LdMNPV IAP-3, whereas common fruit fly and tobacco hornworm did not. PCR with the same fragments of BIR and RING domains of the LdMNPV IAP-3 gene as primers revealed parts of the genomes of gypsy moth, common fruit fly, and tobacco hornworm. Part of the gypsy …
Effects Of Long-Term Exposure Of Female Rats To Low Levels Of Lead: Ovary And Uterus Histological Architecture Changes, Eugenia Dumitrescu, Viorica Chiurciu, Florin Muselin, Roxana Popescu, Diana Brezovan, Romeo Teodor Cristina
Effects Of Long-Term Exposure Of Female Rats To Low Levels Of Lead: Ovary And Uterus Histological Architecture Changes, Eugenia Dumitrescu, Viorica Chiurciu, Florin Muselin, Roxana Popescu, Diana Brezovan, Romeo Teodor Cristina
Turkish Journal of Biology
The aim of the current study was to evaluate lead accumulation in the ovaries, fallopian tubes, and uterus and to take note of any consequent histo-architectural changes. The experiment involved a 12-month chronic exposure of 28 Wistar female rats at sexual maturity (221 ± 0.88 g/individual) to lead acetate in drinking water. The rats were divided into 4 groups based on the level of lead exposure: E1 at 0.050 mg L^{-1}, E2 at 0.100 mg L^{-1}, E3 at 0.150 mg L^{-1} , and a control group that received tap water. Lead level evaluation was performed by atomic absorption spectrometry at …
Enhancing The Expression Of Aspergillus Niger Ss-Mannanase In Pichia Pastoris By Coexpression Of Protein Disulfide Isomerase, Xiaoling Chen, Bo Zhou, Meng Xu, Zhiqing Huang, Gang Jia, Jiayun Qiao, Guangmang Liu
Enhancing The Expression Of Aspergillus Niger Ss-Mannanase In Pichia Pastoris By Coexpression Of Protein Disulfide Isomerase, Xiaoling Chen, Bo Zhou, Meng Xu, Zhiqing Huang, Gang Jia, Jiayun Qiao, Guangmang Liu
Turkish Journal of Biology
A gene encoding ß-mannanase from Aspergillus niger GIM3.452 was amplified and inserted into a pPIC9K vector. The resulting recombinant plasmid, pPIC9K-MAN, was transformed into Pichia pastoris GS115. One strain (GSKM-1) having the highest ß-mannanase activity of 26.6 U/mL was obtained. In order to increase the secretion of ß-mannanase in P. pastoris, we constructed a double recombinant yeast and made it coexpress protein disulfide isomerase. One strain (GSKZ\alphaM2) with the highest ß-mannanase activity of 40 U/mL was then obtained and used to optimize expression conditions. When the GSKZ\alphaM2 strain was induced under the optimized conditions (methanol concentration 1.5%, induction time 7 …
Estradiol Receptors Mediate Estradiol-Induced Inhibition Of Mitochondrialca^{2+} Efflux In Rat Caudate Nucleus And Brain Stem, Snjezana Petrovic, Maja Milosevic, Danijela Ristic-Medic, Natasa Velickovic, Dunja Drakulic, Ivana Grkovic, Anica Horvat
Estradiol Receptors Mediate Estradiol-Induced Inhibition Of Mitochondrialca^{2+} Efflux In Rat Caudate Nucleus And Brain Stem, Snjezana Petrovic, Maja Milosevic, Danijela Ristic-Medic, Natasa Velickovic, Dunja Drakulic, Ivana Grkovic, Anica Horvat
Turkish Journal of Biology
Our earlier studies found that in vitro estradiol modulates mitochondrial Ca2+ transport in discrete brain regions. The present study examined the role of estradiol receptors (ERs) in estradiol-induced inhibition of Ca^{2+} efflux from synaptosomal mitochondria isolated from rat caudate nuclei and brain stems. Radioactively labeled CaCl_2 (0.6?0.75 µCi ^45CaCl_{2}) was used for Ca^{2+} transport monitoring. The results revealed that in the presence of ER antagonist 7\alpha,17ß-[9[(4,4,5,5,5-pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-triene-3,17-diol (ICI 182,780) (1 µmol/L), the inhibitory effect of estradiol on mitochondrial Ca^{2+} efflux was more than 60% decreased, suggesting the involvement of ER in this mode of estradiol neuromodulatory action. When particular contributions of …
Two-Dimensional Gel-Based Serum Protein Profile Of Growing Piglets, Malgorzata Ozgo, Adam Lepczynski, Agnieszka Herosimczyk
Two-Dimensional Gel-Based Serum Protein Profile Of Growing Piglets, Malgorzata Ozgo, Adam Lepczynski, Agnieszka Herosimczyk
Turkish Journal of Biology
In the present study we have introduced a proteomic workflow, 2-dimensional electrophoresis coupled with matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) to create a 2-D map reflecting the characteristic pattern of serum proteins in growing piglets. Blood samples were collected from 6 piglets aged 50 days. Serum proteins were resolved at pH 3-10, followed by 12% SDS-PAGE. Reproducible protein spots (183) were picked from the gels and submitted for identification by peptide mass fingerprinting. As a result, 105 spots were positively identified, representing the products of 37 unique genes. Nearly half of the identified proteins (48.57%) were associated with …
Prokaryotic Expression, Purification, Polyclonal Antibody Preparation, And Tissue Distribution Of Porcine Six1, Meng Xu, Xiaoling Chen, Zhiqing Huang, Wanxue Wen, Shuai Chang, Xiaoyan Wang, Daiwen Chen, Bing Yu, Junqiu Luo, Guangmang Liu
Prokaryotic Expression, Purification, Polyclonal Antibody Preparation, And Tissue Distribution Of Porcine Six1, Meng Xu, Xiaoling Chen, Zhiqing Huang, Wanxue Wen, Shuai Chang, Xiaoyan Wang, Daiwen Chen, Bing Yu, Junqiu Luo, Guangmang Liu
Turkish Journal of Biology
Sine oculis homeobox 1 (Six1), a member of the Six homeoproteins, plays an important role in skeletal myogenesis and the specification of myofiber diversity. In this study, in order to scale up the production of recombinant porcine Six1 (pSix1), a pET-30a(+)-pSix1 plasmid was constructed and transformed into Escherichia coli BL21 (DE3). The recombinant pSix1 could be induced for efficient expression with 2 mM IPTG for 2 h at 30 °C, yielding approximately 4.6 mg/L. The protein was then purified and identified by western blot, and it was used for preparing its polyclonal antibody. The recombinant pSix1 was tagged with a …
A New Record Of Zerconid Mites (Acari, Mesostigmata, Zerconidae) From The Thrace Region Of Turkey, Mehmet Karaca, Raşi̇t Urhan
A New Record Of Zerconid Mites (Acari, Mesostigmata, Zerconidae) From The Thrace Region Of Turkey, Mehmet Karaca, Raşi̇t Urhan
Turkish Journal of Zoology
In this study, Prozercon martae Ujvári, 2010 is recorded for the first time from Turkey. On the basis of the samples collected from the Thrace region of Turkey, its morphological features are given with drawings.
Occurrence Of The Serpent Eel, Ophisurus Serpens (Linnaeus, 1758) (Osteichthyes: Ophichthidae), Close To The Bay Of İzmir (Aegean Sea, Turkey), Ali̇ Ulaş, Okan Akyol
Occurrence Of The Serpent Eel, Ophisurus Serpens (Linnaeus, 1758) (Osteichthyes: Ophichthidae), Close To The Bay Of İzmir (Aegean Sea, Turkey), Ali̇ Ulaş, Okan Akyol
Turkish Journal of Zoology
A specimen of the serpent eel, Ophisurus serpens, with a total length of 1917 mm was caught on 2 March 2014 by an angler on a boat off the coast of Karaburun, in front of the Sarpıncık lighthouse. This specimen is the second record for the central Aegean Sea since that of Aliağa, Çandarlı Bay, in August 1966.
Copidosoma Boucheanum Ratzeburg (Hym.: Encyrtidae): New Record From Iran, Hossein Lotfalizadeh, Mehri Bab-Morad
Copidosoma Boucheanum Ratzeburg (Hym.: Encyrtidae): New Record From Iran, Hossein Lotfalizadeh, Mehri Bab-Morad
Turkish Journal of Zoology
The encyrtid species Copidosoma boucheanum Ratzeburg (Hym.: Chalcidoidea, Encyrtidae) was identified and is reported for the first time from Iran. These specimens were reared from leafroller larvae (Lep.: Tortricidae) on Populus sp. as a polyembryonic endoparasitoid. Copidosoma species of Iran are listed with their distribution.
First Record Of The Genus Diarthrodella Klie, 1949 (Copepoda, Harpacticoida, Paramesochridae) From The Mediterranean Sea, With Description Of A New Species From Turkey, Serdar Sönmez, Suphan Karaytuğ, Serdar Sak
First Record Of The Genus Diarthrodella Klie, 1949 (Copepoda, Harpacticoida, Paramesochridae) From The Mediterranean Sea, With Description Of A New Species From Turkey, Serdar Sönmez, Suphan Karaytuğ, Serdar Sak
Turkish Journal of Zoology
Both sexes of Diarthrodella ergeneae sp. nov. (Copepoda, Harpacticoida, Paramesochridae), which were collected from the intertidal zone of Kurtpınarı Beach along the Mediterranean coast of Turkey, are described in detail. The new species is morphologically most similar to D. convexa Kunz, 1981; D. secunda secunda Kunz, 1954; and D. lancifera Kunz, 1981 according to the setal formula of the swimming legs, but D. ergeneae sp. nov. can easily be differentiated from D. convexa by the ratio of P1 exopod/enp-1, by the lack of an inner seta on P2 enp-2, and by the shape and armature of P5; from D. secunda …
The Genus Glyptholaspis Filipponi & Pegazzano (Acari: Macrochelidae) Of Kelkit Valley (Turkey), With First Description Of Male Of The Species G. Saprophila Mašán, Hasan Hüseyi̇n Özbek, Sali̇h Doğan, Durmuş Ali̇ Bal
The Genus Glyptholaspis Filipponi & Pegazzano (Acari: Macrochelidae) Of Kelkit Valley (Turkey), With First Description Of Male Of The Species G. Saprophila Mašán, Hasan Hüseyi̇n Özbek, Sali̇h Doğan, Durmuş Ali̇ Bal
Turkish Journal of Zoology
This study was carried out on the genus Glyptholaspis in Kelkit Valley, Turkey, during 2007-2012. Three species, G. americana (Berlese, 1888), G. confusa (Foa, 1900), and G. saprophila Masan, 2003, were recorded from Kelkit Valley. G. americana has been previously recorded from Turkey; G. confusa and G. saprophila are first records for the Turkish fauna. The male of G. saprophila is described for the first time in this study. Original drawings for all known species of the genus from Kelkit Valley are included. Additionally, an identification key to the Turkish species of Glyptholaspis is given here.