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An Ethnobotanical Survey In Selected Districts Of The Black Sea Region (Turkey), Emi̇ne Burcu Yeşi̇lyurt, Işil Şi̇mşek, Gali̇p Akaydin, Erdem Yeşi̇lada
An Ethnobotanical Survey In Selected Districts Of The Black Sea Region (Turkey), Emi̇ne Burcu Yeşi̇lyurt, Işil Şi̇mşek, Gali̇p Akaydin, Erdem Yeşi̇lada
Turkish Journal of Botany
The current study was carried out in selected districts of the Black Sea region of Turkey, in Trabzon (center), Çorum (İskilip town), Karabük (Yenice town), Amasya (Kapıkaya village), and Amasya (Belmebük village) in order to document the wild plants used by the local inhabitants, i.e. as a remedy or foodstuff or for other purposes. The data were collected from 352 individuals residing in these districts and volunteering to participate in the research through face-to-face interviewing. Depending on the data collected, the parts of the plants used, the purpose in using them, and the ways of preparation (simply cooking or decoction, …
Studies On The Floral Anatomy And Scent Chemistry Of Titan Arum(Amorphophallus Titanum, Araceae), Vijayasankar Raman, Nurhayat Tabanca, Betül Demi̇rci̇, Ikhlas Khan
Studies On The Floral Anatomy And Scent Chemistry Of Titan Arum(Amorphophallus Titanum, Araceae), Vijayasankar Raman, Nurhayat Tabanca, Betül Demi̇rci̇, Ikhlas Khan
Turkish Journal of Botany
Amorphophallus titanum (Becc.) Becc. ex Arcang., the titan arum, is popularly known for having the world's largest unbranched inflorescence. The enormous flower-like inflorescence is also called 'corpse flower' or 'carrion flower' due to the characteristic putrid odor of the bloom. The present study illustrates detailed anatomy and micromorphology of the inflorescence by light and scanning electron microscopy. Collenchyma was found to be the major tissue providing mechanical support in the spathe. The appendix consisted of a dense parenchymatous cortex enclosing a hollow center traversed by loose networks of aerenchyma. Anthocyanin pigments found in the epidermises and cortical tissues imparted a …
Determining Diurnal And Seasonal Changes In Melatonin Andtryptophan Contents Of Eggplant (Solanum Melongena L.), Ahmet Korkmaz, Gökçen Yakupoğlu, Şebnem Köklü, Yakup Cuci̇, Feri̇t Kocaçi̇nar
Determining Diurnal And Seasonal Changes In Melatonin Andtryptophan Contents Of Eggplant (Solanum Melongena L.), Ahmet Korkmaz, Gökçen Yakupoğlu, Şebnem Köklü, Yakup Cuci̇, Feri̇t Kocaçi̇nar
Turkish Journal of Botany
The objective of this study was to simultaneously determine changes in tryptophan and melatonin contents of eggplant seedlings at two different growth stages during a 24-h period. Variations in tryptophan and melatonin levels in different organs (roots, leaves, fruits, and seeds) and the distribution and accumulation of these molecules in these organs during various growth stages were also investigated. In eggplant seedlings, melatonin showed a strong peak at the beginning of the dark period, while tryptophan levels were at the lowest. Melatonin was also high in the middle of the light period and an inverse relationship was observed between melatonin …
Karyotype Analysis Of Some Taxa Of Dianthus Section Verruculosi (Caryophyllaceae, Sileneae), Derya Altay, Hali̇l Erhan Eroğlu, Ergi̇n Hamzaoğlu, Murat Koç
Karyotype Analysis Of Some Taxa Of Dianthus Section Verruculosi (Caryophyllaceae, Sileneae), Derya Altay, Hali̇l Erhan Eroğlu, Ergi̇n Hamzaoğlu, Murat Koç
Turkish Journal of Botany
The purpose of this study was to determine chromosome numbers and karyological features of six Dianthus taxa belonging to section Verruculosi from Anatolia, Turkey. The chromosome numbers were determined as 2n = 30 in all taxa. Karyological measurements were made in detail for the first time for all taxa. Chromosome counts were given for the first time for all taxa except D. cyri and D. strictus. A new chromosome number was determined for D. strictus. The intrachromosomal and interchromosomal karyotype asymmetries were estimated with MCA and CVCL values.
Zn-Use Efficiency For Optimization Of Symbiotic Nitrogenfixation In Chickpea (Cicer Arietinum L.), Igor Kryvoruchko
Zn-Use Efficiency For Optimization Of Symbiotic Nitrogenfixation In Chickpea (Cicer Arietinum L.), Igor Kryvoruchko
Turkish Journal of Botany
Zn deficiency is widespread in traditional areas of chickpea cultivation worldwide. It limits chickpea productivity and causes significant losses to the economies of the world?s largest chickpea exporters. This review may be of interest to researchers who would like to contribute to the improvement of chickpea cultivation on Zn-depleted soils in an environmentally sustainable manner, namely via identification of genotypes with superior symbiotic performance under Zn-limited conditions. The primary aim of the current work is to familiarize the readers with the biology and symbiotic characteristics of chickpea, and also to provide the necessary background on Zn as an essential nutrient …
Yuhina Nigrimenta Blyth (Zosteropidae) As A Bird Pollinator Ofbrandisia Hancei Hook.F. (Scrophulariaceae) During Winter, Yifan Qian, Yun Xiang Li, Xuemei Zhang, Qiu Mei Quan
Yuhina Nigrimenta Blyth (Zosteropidae) As A Bird Pollinator Ofbrandisia Hancei Hook.F. (Scrophulariaceae) During Winter, Yifan Qian, Yun Xiang Li, Xuemei Zhang, Qiu Mei Quan
Turkish Journal of Botany
Winter-flowering plants commonly attract an insufficient number of pollinators. Harsh climate conditions, such as low temperature, make it impossible for insects to evolve as reliable pollinators. Birds can buffer against changes in weather to be more reliable pollinators for these kinds of plants, such as Brandisia hancei Hook.f. (Scrophulariaceae), a shrub endemic to the Northeast Sichuan Basin in China. In this study, the flower visitors, flowering phenology, pollinators, breeding system, and nectar availability of B. hancei Hook.f. were investigated. Results show that the flowering period duration was approximately 3 months for the whole population and more than 10 days per …
Allelic Variations Of Glutenin Subunits And Their Associationwith Quality Traits In Bread Wheat Genotypes, Hüsnü Aktaş, Faheem Shehzad Baloch
Allelic Variations Of Glutenin Subunits And Their Associationwith Quality Traits In Bread Wheat Genotypes, Hüsnü Aktaş, Faheem Shehzad Baloch
Turkish Journal of Agriculture and Forestry
The present study was conducted to evaluate the genotype x environment interaction of the yield and quality traits for five bread wheat varieties commonly grown in the Southeastern Anatolia Region of Turkey and 20 advanced lines developed within the framework of the International Winter Wheat Improvement Project. We also determined the allelic pattern of the Glu-1 and Glu-3 loci of these genotypes and examined whether these loci had an effect on the quality traits. There was a significant variation among the genotypes and environments in terms of grain yield, protein content, sedimentation volume (SV), and the extensograph dough energy value …
Proteomics Analysis Of Mitochondrial Dysfunction Triggered By Complex Specific Electron Transport Chain Inhibitors Reveals Common Pathways Involving Protein Misfolding In An Sh-Sy5y In Vitro Cell Model, Betül Şahi̇n, Ahmet Tarik Baykal
Proteomics Analysis Of Mitochondrial Dysfunction Triggered By Complex Specific Electron Transport Chain Inhibitors Reveals Common Pathways Involving Protein Misfolding In An Sh-Sy5y In Vitro Cell Model, Betül Şahi̇n, Ahmet Tarik Baykal
Turkish Journal of Biology
Mitochondrial dysfunction has been previously identified in neurodegenerative diseases such as Alzheimer disease, Huntington disease, and Parkinson disease. Chemical inhibition of the mitochondrial electron transport chain (ETC) was shown to trigger symptoms in animal models similar to those observed in human neurodegenerative diseases. In order to understand the effect of mitochondrial dysfunction on the proteome level, LC-MSE-based bottom-up, label-free differential proteomics expression analysis was used to monitor protein level changes in SH-SY5Y neuroblastoma cells induced by ETC-specific inhibitors (MPTP, 3-NP, sodium azide, antimycin A, and oligomycin). A total of 379 proteins were identified across the sample set and 75 of …
Interaction Of Adipose-Derived Mesenchymal Stem Cells With Mcf-7 Cells In Vitro:A Study Emphasizing Signaling Molecule Expression And Transcriptional Changes, Qiong Wu, Chang-Xin Jin, Hui Chen, Xue-Yong Li, Yuejin Li
Interaction Of Adipose-Derived Mesenchymal Stem Cells With Mcf-7 Cells In Vitro:A Study Emphasizing Signaling Molecule Expression And Transcriptional Changes, Qiong Wu, Chang-Xin Jin, Hui Chen, Xue-Yong Li, Yuejin Li
Turkish Journal of Biology
Adipose-derived stem cells (ADSCs) promote metastasis of breast cancer cells that can differentiate into carcinoma-associated cells in tumor microenvironments. However, the precise mechanism is poorly understood. This study shows that interaction of ADSCs with breast cancer MCF-7 cells changes the level of metastasis-related functional proteins in MCF-7 cells, as well as that of oncogenes in ADSCs. ADSCs were isolated from adipose tissues of patients. The interaction of ADSCs with MCF-7 cells was performed by coculturing ADSCs (or MCF-7 cells) with exosomes derived from MCF-7 cells (or ADSCs). Exosomes were labeled by DiI. In cocultures, migration-related regulators in MCF-7 cells were …
Optimizations Needed For Lateral Flow Assay For Rapid Detection Of Pathogenic E. Coli, Di̇lek Çam, Hüseyi̇n Avni̇ Öktem
Optimizations Needed For Lateral Flow Assay For Rapid Detection Of Pathogenic E. Coli, Di̇lek Çam, Hüseyi̇n Avni̇ Öktem
Turkish Journal of Biology
Lateral flow assay (LFA), or the immunochromatographic strip test, is popular to use for rapid and sensitive immunoassays. Gold nanoparticles (GNPs), due to tunable optical characteristics and easy manipulation of size or shape, represent an attractive approach for LFA technology. Since most enterohemorrhagic infections result from water and food contaminations of Escherichia coli O157:H7, selective and rapid detection of this organism in environmental and biological complexes is necessary. In this study, optimized parameters of antibody (Ab)-based LFA for rapid detection of pathogenic E. coli O157:H7 are described. GNPs were used as visualizing agents. The measuring parameters include the Ab concentration …
Gelatin Positively Regulates The Immunosuppressive Capabilitiesof Adipose-Derived Mesenchymal Stem Cells, Imdad Ullah Khan, Yongseok Yoon, Wan Hee Kim, Oh-Kyeong Kweon
Gelatin Positively Regulates The Immunosuppressive Capabilitiesof Adipose-Derived Mesenchymal Stem Cells, Imdad Ullah Khan, Yongseok Yoon, Wan Hee Kim, Oh-Kyeong Kweon
Turkish Journal of Biology
The characteristics of adipose-derived mesenchymal stem cells (Ad-MSCs) can be selectively enhanced by altering the culture environment. We evaluated the effects of gelatin on Ad-MSCs when used in combination with culture media. Ad-MSCs were initially cultured in 0%, 0.5%, 1%, 2%, and 4% gelatin in Dulbecco's modified Eagle's medium (DMEM) to evaluate cell proliferation. The expression of inflammatory, antiinflammatory, antioxidant, and osteogenic markers was then assessed by rtPCR in Ad-MSCs cultured in 0.5% gelatin in DMEM (GMSCs), and without gelatin (MSCs), for 5 and 10 days. We found that 0.5% gelatin significantly increased the proliferation rate of Ad-MSCs after 24 …
Improved Insulin-Secreting Properties Of Pancreatic Islet Mesenchymalstem Cells By Constitutive Expression Of Pax4 And Mafa, Ayşegül Açiksari, Gökhan Duruksu, Erdal Karaöz
Improved Insulin-Secreting Properties Of Pancreatic Islet Mesenchymalstem Cells By Constitutive Expression Of Pax4 And Mafa, Ayşegül Açiksari, Gökhan Duruksu, Erdal Karaöz
Turkish Journal of Biology
For long-term treatment of diabetes type 1, transplantation of insulin-producing beta cells may be a promising method, but the limited number of islets for transplantation requires the development of different approaches. In this study, we aimed to generate beta-like insulin-producing cells. For this purpose, MafA, Pax4, and Ngn3 genes were transferred into pancreatic islet-derived mesenchymal stem cells, and the effect of their ectopic expressions on differentiation efficiency was examined. Stemness properties of pancreatic islet stem cells were characterized. The 3 genes were transfected by electroporation and expressed constitutively. The transfected cells were further stimulated to differentiate by using chemical induction. …
Influence Of Vermicompost Application On Soil Consistency Limits And Soil Compactibility, Serdar Sari, Ekrem Lütfi̇ Aksakal, İlker Angin
Influence Of Vermicompost Application On Soil Consistency Limits And Soil Compactibility, Serdar Sari, Ekrem Lütfi̇ Aksakal, İlker Angin
Turkish Journal of Agriculture and Forestry
Soil organic matter (SOM) is a composite indicator of soil quality. Recycling of organic wastes is one of the environmentally compatible and economically viable options to increase and/or maintain SOM content. Our study was conducted to evaluate the effects of vermicompost (0%, 0.5%, 1%, 2%, and 4% w/w) on the consistency limits and the Proctor compaction test parameters of three texturally different soils (sandy loam, loam, and clay) from similar climatic conditions and cropping management practices. Results showed that vermicompost increased the optimum moisture content (OMC) with an associated decrease in maximum dry soil bulk density (MBD). The increase in …
Chemical Composition And Morphometric Traits And Yield Ofcarrots Grown In Organic And Integrated Farming Systems, Natasa Sink, Maja Mikulic Petkovsek, Robert Veberic, Nina Kacjan Marsic
Chemical Composition And Morphometric Traits And Yield Ofcarrots Grown In Organic And Integrated Farming Systems, Natasa Sink, Maja Mikulic Petkovsek, Robert Veberic, Nina Kacjan Marsic
Turkish Journal of Agriculture and Forestry
Changes in chemical composition and quality traits of carrot roots (Daucus carota L.) grown in organic (ORG) and integrated (INT) farming systems were investigated during two successive years. Three carrot cultivars (Fanal, Rodelika, and Rolanka) were included. Determinations of sugars, organic acids, α- and ß-carotene, and vitamin C were performed with an HPLC system, and a liquid chromatography-mass spectrometry (LC-MS) system was used for the identification and quantification of phenolics. A higher yield of carrot in the ORG system was observed (42-44 t ha-1) as compared with the INT system (37-40 t ha-1). The impact of farming system on morphometric …
The Effects Of Different Plant Densities And Silage Corn Varieties On Silage Yield And Some Yield Parameters In No-Till Seeding, Mehmet Zahi̇d Malasli, Ali̇ Khatami̇, Ahmet Çeli̇k
The Effects Of Different Plant Densities And Silage Corn Varieties On Silage Yield And Some Yield Parameters In No-Till Seeding, Mehmet Zahi̇d Malasli, Ali̇ Khatami̇, Ahmet Çeli̇k
Turkish Journal of Agriculture and Forestry
In this study, the possibility of growing silage corn, which is used as an important nutrient in livestock and whose production is increasing in recent years, by no-till seeding method under Erzurum conditions was investigated. For this purpose, three silage corn varieties (DKC-5783, Prestige, and ADA-9510) suitable for Erzurum's climatic conditions in terms of vegetation period were selected from a large number of silage corn seeds widely grown throughout Turkey and their development conditions under no-till seeding conditions were investigated. A precision vacuum no-till seeder with double disk type furrow opener was used for silage corn seeding. Based on three …
The Jasmonate-Zim Domain Protein Ftjaz2 Interacts With The R2r3-Mybtranscription Factor Ftmyb3 To Affect Anthocyanin Biosynthesis In Tartary Buckwheat, Xiao-Peng Luo, Shuang-Jiang Li, Pan-Feng Yao, Cheng-Lei Li, Hui Chen, Qi Wu, Hai-Xia Zhao
The Jasmonate-Zim Domain Protein Ftjaz2 Interacts With The R2r3-Mybtranscription Factor Ftmyb3 To Affect Anthocyanin Biosynthesis In Tartary Buckwheat, Xiao-Peng Luo, Shuang-Jiang Li, Pan-Feng Yao, Cheng-Lei Li, Hui Chen, Qi Wu, Hai-Xia Zhao
Turkish Journal of Biology
Tartary buckwheat is a type of coarse cereal that contains a rich supply of flavonoids. When suffering from certain stresses, tartary buckwheat can respond by promoting the biosynthesis of anthocyanins. It is well established that the jasmonate (JA) signaling pathway plays a critical role in plant resistance to disease by stimulating the production of anthocyanins; furthermore, the genes encoding the jasmonate-ZIM domain (JAZ) proteins have been shown to be key players in this process. However, the role of JAZ proteins in tartary buckwheat is poorly understood. In this study, we aimed to isolate FtJAZs from tartary buckwheat and identify FtJAZs …
An In Vitro Human Skeletal Muscle Model: Coculture Of Myotubes,Neuron-Like Cells, And The Capillary Network, Ayşe Burcu Ertan, Hali̇me Kenar, Tahsi̇n Beyza Beyzadeoğlu, Fatma Neşe Kök, Gamze Köse
An In Vitro Human Skeletal Muscle Model: Coculture Of Myotubes,Neuron-Like Cells, And The Capillary Network, Ayşe Burcu Ertan, Hali̇me Kenar, Tahsi̇n Beyza Beyzadeoğlu, Fatma Neşe Kök, Gamze Köse
Turkish Journal of Biology
This study reports the generation of a new human muscle tissue equivalent from skeletal muscle-derived stem cells and human umbilical vein endothelial cells (HUVECs). Skeletal muscle stem cells were isolated by the preplate technique and differentiated into neuron-like cells that were positive for neuronal beta-tubulin3 and nestin and negative for the astrocyte marker glial fibrillary acidic protein (GFAP). Coculture of skeletal muscle stem cells with the HUVECs under optimized fetal bovine serum and media conditions resulted in formation of a capillary network among the multinucleated myotubes. The neuron-like cells derived from the human skeletal muscle stem cells were seeded onto …
A Novel Optical Flow-Based Representation For Temporal Video Segmentation, Samet Akpinar, Ferdanur Alpaslan
A Novel Optical Flow-Based Representation For Temporal Video Segmentation, Samet Akpinar, Ferdanur Alpaslan
Turkish Journal of Electrical Engineering and Computer Sciences
Temporal video segmentation is a field of multimedia research enabling us to temporally split video data into semantically coherent scenes. In order to develop methods challenging temporal video segmentation, detecting scene boundaries is one of the more widely used approaches. As a result, representation of temporal information becomes important. We propose a new temporal video segment representation to formalize video scenes as a sequence of temporal motion change information. The idea here is that some sort of change in the optical flow character determines motion change and cuts between consecutive scenes. The problem is eventually reduced to an optical flow-based …
Data Clustering Using Seed Disperser Ant Algorithm, Wen Liang Chang, Jeevan Kanesan, Anand Jayant Kulkarni, Harikrishnan Ramiah
Data Clustering Using Seed Disperser Ant Algorithm, Wen Liang Chang, Jeevan Kanesan, Anand Jayant Kulkarni, Harikrishnan Ramiah
Turkish Journal of Electrical Engineering and Computer Sciences
Nature-inspired optimization algorithms have become popular in the past decade. They have been applied to solve various kinds of problems. Among these would be data clustering, which has become popular in data mining in recent times due to the data explosion. In the last decade, many metaheuristic algorithms have been used to obtain improved data clustering optimization for solving data mining problems. In this paper, we applied the seed disperser ant algorithm (SDAA), which mimics the evolution of an Aphaenogaster senilis ant colony, and we introduced a modified SDAA that is a hybrid of K-means and SDAA for solving data …
On The Performance Of Newsworthy Meta-Heuristic Algorithms Based On Point Of View Fuzzy Modelling, Ci̇han Karakuzu
On The Performance Of Newsworthy Meta-Heuristic Algorithms Based On Point Of View Fuzzy Modelling, Ci̇han Karakuzu
Turkish Journal of Electrical Engineering and Computer Sciences
Heuristic algorithms are used for optimizing a large diversity of engineering problems. In this study, the performance of six commonly used and cited meta-heuristic algorithms (MhAs) is compared based on point of view fuzzy modelling. In this study, the simplest version of MhAs is specifically used to observe and evaluate their natural optimization performances. For the comparisons, dynamic system modelling using a neuro-fuzzy system (NFS) was studied. Obtained results were evaluated based on commonly used statistical metrics, and then each algorithm was ranked according to these metrics. Based on the computed average ranks, the best MhA among the six MhAs …
Power Search Algorithm (Psa) For Combined Economic-Emission Dispatch Problems Considering Valve Point Effects In Economic Load Dispatch, Prakash Arumugam, Ravichandran Coimbatore Subramanian
Power Search Algorithm (Psa) For Combined Economic-Emission Dispatch Problems Considering Valve Point Effects In Economic Load Dispatch, Prakash Arumugam, Ravichandran Coimbatore Subramanian
Turkish Journal of Electrical Engineering and Computer Sciences
Economic load dispatch has become the prime and major concern in power system networks. Use of nonrenewable fuels as an input to power systems results in toxic gas emission and depletion of resources for future generations. Allocation of optimal power to generators serves as a solution to this issue. The suggested power search algorithm (PSA) is a novel solution for the reduction of the fuel cost, emissions, and power loss. This algorithm searches its solution inside the search space from minimum to maximum in case of forward search. The global optimum solution is obtained with much less execution time compared …
Nitric Oxide In Cerebrospinal Fluid Of Central Nervous System Tuberculosis: Correlations With Culture, Antibody Response, And Cell Count, Kavitha Kumar, Prashant Giribhattanavar, Shripad Patil
Nitric Oxide In Cerebrospinal Fluid Of Central Nervous System Tuberculosis: Correlations With Culture, Antibody Response, And Cell Count, Kavitha Kumar, Prashant Giribhattanavar, Shripad Patil
Turkish Journal of Medical Sciences
Background/aim: The role of nitric oxide (NO) has been established in infection over the years. NO functions by inhibiting the growth of intracellular pathogens. The present study was undertaken to ascertain the role of NO in central nervous system (CNS) infection by Mycobacterium tuberculosis. Materials and methods: A total of 781 chronic meningitis cerebrospinal fluid (CSF) samples suspected of CNS tuberculosis (TB) were categorized based on M. tuberculosis culture positivity, anti-TB antibody response, and CSF cell count and were analyzed for NO. Results: We found that NO levels were positive in 10.88% of the CSF samples. Positivity for NO was …
Epidemiology And Molecular Characteristics Of Methicillin-Resistantstaphylococcus Aureus From Skin And Soft Tissue Infections In Shiraz, Iran, Samira Mehrshad, Masoud Haghkhah, Shahin Aghaei
Epidemiology And Molecular Characteristics Of Methicillin-Resistantstaphylococcus Aureus From Skin And Soft Tissue Infections In Shiraz, Iran, Samira Mehrshad, Masoud Haghkhah, Shahin Aghaei
Turkish Journal of Medical Sciences
Background/aim: Panton-Valentine leukocidin (PVL)-positive methicillin-resistant Staphylococcus aureus (MRSA) infections are increasing in some regions of Iran. The aim of the current study was to assess the epidemiology and molecular characteristics of S. aureus isolated from patients with skin infections in Shiraz, Iran. Materials and methods: Swab samples were obtained from patients admitted to the skin and burn units of hospitals. The medical records of each patient were collected via questionnaire. All staphylococcal isolates were collected and examined by conventional methods for detecting S. aureus strains. PCR was used to detect S. aureus harboring the mecA and pvl genes. Results: Out …
Physicochemical And Biochemical Parameters In Milk Of Serbian Breastfeeding Women, Slavica Sunaric, Marko Denic, Jelena Lalic, Tatjana Jovanovic, Ana Spasic, Jelena Zivkovic, Natasa Trutic, Gordana Kocic
Physicochemical And Biochemical Parameters In Milk Of Serbian Breastfeeding Women, Slavica Sunaric, Marko Denic, Jelena Lalic, Tatjana Jovanovic, Ana Spasic, Jelena Zivkovic, Natasa Trutic, Gordana Kocic
Turkish Journal of Medical Sciences
Background/aim: This study was undertaken to determine the changes and relationships between some important milk constituents as well as physical, rheological, and biochemical parameters of milk obtained from Serbian breastfeeding mothers. Materials and methods: Physicochemical and biochemical parameters and the concentrations of vitamins, uric acid, and minerals were determined during the three periods of lactation covering colostrum, transitional, and mature milk collected from 67 mothers who had a term-pregnancy. Results: Large interindividual variations regarding many parameters were found between mothers at the same period of lactation, but the average values were mostly in the expected and recommended ranges. For some …
The Role Of Oxidative Stress In α-Amanitin-Induced Hepatotoxicityin An Experimental Mouse Model, Zerri̇n Defne Dündar, Mehmet Ergi̇n, İbrahi̇m Kilinç, Tamer Çolak, Pembe Oltulu, Başar Cander
The Role Of Oxidative Stress In α-Amanitin-Induced Hepatotoxicityin An Experimental Mouse Model, Zerri̇n Defne Dündar, Mehmet Ergi̇n, İbrahi̇m Kilinç, Tamer Çolak, Pembe Oltulu, Başar Cander
Turkish Journal of Medical Sciences
Background/aim: This study aimed to evaluate oxidative stress markers of liver tissue in a mouse α-amanitin poisoning model with three different toxin levels. Materials and methods: The mice were randomly divided into Group 1 (control), Group 2 (0.2 mg/kg), Group 3 (0.6 mg/kg), and Group 4 (1.0 mg/kg). The toxin was injected intraperitoneally and 48 h of follow-up was performed before sacrifice. Results: Median superoxide dismutase activities of liver tissue in Groups 3 and 4 were significantly higher than in Group 1 (for both, P = 0.001). The catalase activity in Group 2 was significantly higher, but in Groups 3 …
The Role Of Outer Inflammatory Protein A (Oipa) In Vaccination Of Thec57bl/6 Mouse Model Infected By Helicobacter Pylori, Mohaddese Mahboubi, Tahereh Falsafi, Majid Sadeghizadeh, Fatemeh Mahjoub
The Role Of Outer Inflammatory Protein A (Oipa) In Vaccination Of Thec57bl/6 Mouse Model Infected By Helicobacter Pylori, Mohaddese Mahboubi, Tahereh Falsafi, Majid Sadeghizadeh, Fatemeh Mahjoub
Turkish Journal of Medical Sciences
Background/aim: Outer inflammatory protein A (OipA) is an important adhesin of Helicobacter pylori. Our goal was to assess the role of OipA in protection of C57BL/6 mice against H. pylori. Materials and methods: C57BL/6 mice were mucosally immunized with recombinant OipA protein, OipA + propolis, propolis, and phosphate-buffered saline. After vaccination, anti-OipA IgA was measured. Mice were challenged three times with 5 x 107 CFU of the H. pylori B19 strain. Two weeks later, bacterial colonization and inflammation in the stomach was analyzed using standard methods. Results: The CFU number in the OipA group was significantly (P < 0.05) lower than that of the control. The CFU number in the OipA + propolis group was higher than those of the OipA and propolis groups. IgA titers were significantly higher (P < 0.0001) in the OipA group compared to the control and OipA + propolis groups. Propolis did not play an adjuvant effect but it interfered with the efficient vaccine effect of OipA. Conclusion: Results show the effect of vaccination by OipA in protection of the mouse model and the importance of OipA in H. pylori pathogenesis. OipA may be proposed as a suitable oral vaccine candidate against H. pylori infection; however, further study is required to determine adjuvant or adverse effects of propolis toward OipA.
The Effect Of Kisspeptin On Spermatogenesis And Apoptosis In Rats, Ni̇lüfer Aytürk, Tüli̇n Firat, Aysel Kükner, Candan Özoğul, Fatma Töre, İsmai̇l Engi̇n Kandirali, Bayram Yilmaz
The Effect Of Kisspeptin On Spermatogenesis And Apoptosis In Rats, Ni̇lüfer Aytürk, Tüli̇n Firat, Aysel Kükner, Candan Özoğul, Fatma Töre, İsmai̇l Engi̇n Kandirali, Bayram Yilmaz
Turkish Journal of Medical Sciences
Background/aim: To study the effect of kisspeptin, a gonadotropin release stimulator, on the testicular tissue of the rat. Materials and methods: Four groups were formed as follows: control, Kiss-10 50 nmol administration for 1 day, Kiss-10 administration for 13 days, and one last group kept for 7 days following Kiss-10 applied for 13 days. Testicular tissues were stained with hematoxylin-eosin, periodic acid Schiff, Masson trichrome staining, terminal deoxynucleotidyl transferased UTP nick-end labeling, and Ki-67 immune staining. Serum testosterone levels were determined. Results: Serum testosterone level increased following acute application, while it was reduced by chronic treatment. Spermatogenic cells as stained …
Metoclopramide Inhibits Trigeminovascular Activation:Evidence For Effective Acute Attack Treatment In Migraine, Hacer Doğanay Aydin, Doğa Vuralli, Di̇dem Tuba Akçali, Hayrunni̇sa Bolay Belen
Metoclopramide Inhibits Trigeminovascular Activation:Evidence For Effective Acute Attack Treatment In Migraine, Hacer Doğanay Aydin, Doğa Vuralli, Di̇dem Tuba Akçali, Hayrunni̇sa Bolay Belen
Turkish Journal of Medical Sciences
Background/aim: Metoclopramide is an effective and commonly used medication in acute migraine treatment but an experimental evidence base is lacking. We aimed to investigate the antimigraine effect of metoclopramide in a migraine model and whether the analgesic effect of metoclopramide was likely to be D2 receptor-mediated. Materials and methods: Cortical spreading depression (CSD) was used to model migraine in adult male Wistar rats. Five CSDs were induced by pinprick. Metoclopramide (two different doses), raclopride, or 0.9% saline were administered 30 min before CSD induction. Two hours after the experiments, brain tissues were examined for c-fos activation. Results: In metoclopramide groups …
Performing Icsi With Commercial Microinjection Pipettes Enhanced Pregnancy Rates, Mohammad Ali Khalili, Iman Halvaei, Shahin Ghazali, Mohammad Hossein Razi
Performing Icsi With Commercial Microinjection Pipettes Enhanced Pregnancy Rates, Mohammad Ali Khalili, Iman Halvaei, Shahin Ghazali, Mohammad Hossein Razi
Turkish Journal of Medical Sciences
Background/aim: Many technical factors can affect intracytoplasmic sperm injection (ICSI) outcomes. The role of the injection micropipette could be of vital importance in ICSI programs. The main goal was to compare ICSI pregnancy outcomes between commercial and home-made injection micropipettes in a large population with male factor infertility. Materials and methods: Five-hundred and eleven ICSI cycles with severe male factor were included in this retrospective study. ICSI cycles were divided into two groups: A (home-made micropipettes, n = 267) and B (commercial micropipettes, n = 244). Rates of fertilization, embryo formation, and chemical and clinical pregnancies were compared between the …
Routine Assessment Of Occupational Exposure And Its Relation To Semen Quality In Infertile Men: A Cross-Sectional Study, Salima Daoud, Afifa Sellami, Mehdi Bouassida, Sahbi Kebaili, Leila Ammar Keskes, Tarek Rebai, Nozha Chakroun Feki
Routine Assessment Of Occupational Exposure And Its Relation To Semen Quality In Infertile Men: A Cross-Sectional Study, Salima Daoud, Afifa Sellami, Mehdi Bouassida, Sahbi Kebaili, Leila Ammar Keskes, Tarek Rebai, Nozha Chakroun Feki
Turkish Journal of Medical Sciences
Background/aim: Concerns about the detrimental effects of occupational and environmental exposure on male reproductive function have been raised by reports of declining sperm quality over the last decades. The aim of this study was to investigate the association between altered semen parameters and exposure to occupational risk factors as assessed by questionnaire. Materials and methods: We conducted a cross-sectional questionnaire-based study among a population of 2122 men who underwent andrological investigation for couple infertility. All participants were interviewed and their semen samples were analyzed. Information about medical history and occupational exposure was used to classify participants into exposed and unexposed …