Digital Commons Network^™

Evaluation Of A Direct Fed Microbial An An Enzymatically Hydrolyzed Yeast Product Fed Alone Or In Combination To Beef Steers Administered Ractopamine Hydrochloride 28 Days Prior To Harvest During Summer Months In The Northern Plains, Erin Gubbels, Forest Francis, Thomas G. Hamilton, Jason Griffin, Warren Rusche, Tom Rehberger, Elliot Block, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Single-sourced, newly weaned steers (n=256; initial BW=542 ± 3.7lb; n=64 steers/treatment; 8 steers/pen) were blocked by location in a 2×2 factorial arrangement of DFM (Certillus CP B1801 Dry; Bacillus subtilis, Lactobacillus plantarum; 28 g/steer·d-1) and YCW (Celmanax; 18 g/steer·d-1). Temperature-humidity index (THI) was calculated as: THI=0.81×ambient temperature+[relative humidity×(ambient temperature-14.40)]+46.40. On d-1 and 2 and d-21 and 22 on RH, respiration rate (RR) and panting scores (PS) were determined before and after AM and PM feedings (0700h, 1100h, 1400h, 1700h). RR (n=3 steers/pen) was calculated from: 600/seconds required for 10 flank movements. PS utilized this scoring system: 0 (not …

Effects Of On-Arrival Application Of A Modified-Live Respiratory And Clostridia Vaccination On Health, Growth Performance, And Antibody Titers Of Newly-Weaned Calves, Erin Gubbels, Santana Hanson, Tommy Norman, Thiago Ribeiro, Thomas G. Hamilton, Forest Francis, Jason Griffin, Warren Rusche, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Single-sourced, newly weaned steers (n=70; initial body weight (BW)=560±12.9lb) were allotted to 10 pens (n=5 pens/treatment with 7 steers/pen). Steers were blocked by BW in a randomized complete block design of VAC [vaccinated for IBR, BVD 1 and 2, PI3, and BRSV (Bovi-Shield Gold 5, Zoetis, Parsippany, NJ) and clostridial (Ultrabec 7/Somubac, Zoetis) upon arrival] or NOVAC (not vaccinated for IBR, BVD 1 and 2, PI3, and BRSV or clostridial species upon arrival). Steers were individually weighed on d 0 (arrival), 1, 21, and 42 for growth performance measures. Whole blood samples (10 mL) were collected (n=3 steers/pen …

Principles Of Holistic Resource Management: Investing In Self-Education, Pete Bauman

SDSU Beef Day 2022 Summary Publication

Study Description:

First developed as a concept for approaching grazing resources management by Alan Savory in the 1970’s as a means of combating poor grazing practices, the concept of Holistic Management has evolved in the modern era to Holistic Resource Management, or in some cases “Wholistic” Resource Management, suggesting a more inclusive approach to overall planning and assessment beyond developing a sustainable grazing plan.

In simplified terms, a complete Holistic Resource Management plan for an agricultural businesses ultimately embraces a core concept that centers on the original ‘Triple Bottom Line’ accounting philosophy where the three “P’s” of profit, people, and …

Use Of Pregnancy Associated Glycoproteins To Determine Fetal Age Throughout Gestation And Clearance Rate In Postpartum Beef Cattle, Adalaide C. Kline, Julie Walker, Taylor N. Andrews, Saulo Menegatti Zoca, Kaitlin M. Epperson, Lacey K. Quail, Jerica J.J. Rich, Jim R. Rhoades, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Previously identified pregnant females from four different herds and postpartum females from one herd were utilized. Blood samples were collected (n = 1,753; study 1) between d 28 and 285 of gestation and (heifers n = 418 and cows n = 657; study 2) once a week for up to 12 weeks after calving. Serum was tested in duplicate using a commercially available blood pregnancy test, IDEXX Alertys Pregnancy Test. In study 1, procedures were modified to allow PAG concentrations to fall within the detectible range of the assay. Data were analyzed using the MIXED procedure of SAS …

Comparison Of Lateral Flow To Other Pregnancy Determination Methods In Order To Determine Accuracy Of Pregnancy Status In Beef Cattle Pre And Postpartum, Adalaide C. Kline, Julie Walker, Taylor N. Andrews, Saulo Menegatti Zoca, Kaitlin M. Epperson, Lacey K. Quail, Jaclyn N. Ketchum, Jerica J.J. Rich, Jim R. Rhoades, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Blood samples were collected from six different Bos taurus herds between day 27 and 285 of gestation (heifers n = 1,205 and cows n = 1,539). Blood samples to determine PAG clearance interval were collected weekly postpartum for up to 12 weeks (heifers n = 418 and cows n = 657). Serum was tested using the lateral flow test and were read by two technicians who were blind to pregnancy status. Level of agreement between the tests were determined by Pearson’s correlation coefficients and Kappa scores. The MIXED procedure of SAS was used to evaluate the effect of …

Use Of Sperm In Vitro Capacitation And Flow Cytometry To Estimate Bull Fertility, Saulo Menegatti Zoca, Thomas W. Geary, Abigail L. Zezeski, Karl C. Kerns, Joseph C. Dalton, Bo R. Harstine, Matthew D. Utt, Robert A. Cushman, Julie Walker, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Frozen-thawed semen from five bulls previously identified as high (48.1% and 47.7%, bulls A and B, respectively), intermediary (45.5%, bull C) or low (43.1% and 40.7%, bulls D and E, respectively) fertility, based on pregnancy per AI, were evaluated with several laboratory measures. Measures included total motility, sperm plasma membrane integrity (viability), acrosome integrity, reactive oxygen species (ROS), mitochondrial membrane energy potential (mito-potential), zinc signatures (signatures 1 to 4), and CD9 protein populations at pre-wash, post-wash, h 0 (diluted with non-capacitation media), and at 0, 3, 6, and 24 h after dilution with capacitation media and incubation at …

Relationship Of Dag1 And Serpina5 Sperm Proteins With Bull Fertility, Saulo Menegatti Zoca, Julie Walker, Jerica J. J. Rich, Kaitlin M. Epperson, Taylor N. Andrews, Adalaide C. Kline, Jessica Nora Drum, M. Sofia Ortega, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Semen from 22 dairy bulls was used to evaluate the presence, localization, and quantification of DAG1 and SERPINA5 on sperm. Sperm motility parameters and viability was also evaluated for semen from each bull. Semen from 19 out of the 22 dairy bulls was used for in vitro embryo production (two Low-SCR and one High-SCR were not available for in vitro embryo production). Bulls were classified based on their sire conception rates (SCR) values as High-SCR (SCR > 1.0) or Low-SCR fertility (SCR < -4.0). Low fertility bulls were subdivided based on their blastocyst rate (BL) as High-BL (Low-SCR/High-BL BL ≥ 31%) or Low-BL (Low-SCR/Low-BL BL ≤ 26%), and High-SCR bulls were not subdivided. The GLM procedure in SAS was used with bull as a fixed effect to determine if variance was greater between bulls compared to within bull. Correlations were determined among DAG1 and SERPINA5 concentrations, percentage of tail labeled for SERPINA5, SCR, sperm total motility, progressive motility, and viability, and in vitro embryo produced cleavage rate (CL) and BL. The GLIMMIX procedure of SAS was used to evaluate the relationship of bull field fertility (High- and Low-SCR), and field and in vitro fertility (High-SCR, Low-SCR/High-BL, Low-SCR/Low-BL) classifications with sperm total (TMOT) and progressive (PROG) motility, viability, CL, BL, DAG1 and SERPINA5 relative concentration, and proportion of sperm tail labeled for SERPINA5. Both SERPINA5 and DAG1 were localized on the sperm head; however, SERPINA5 was also localized on the sperm tail. There was greater variance in concentration among bulls compared to within bull for both DAG1 (P < 0.01; 69.4 vs 49.1, respectively) and SERPINA5 (P < 0.01; 325.8 vs 285.4, respectively). There was a positive correlation between concentration of DAG1 and SERPINA5 (P = 0.01; r = 0.54). Concentrations of SERPINA5 were also correlated with CL (P = 0.04; r = 0.48), and percentage of sperm tail labeled for SERPINA5 was correlated with viability (P = 0.05; r = 0.44) and tended to be correlated with CL (P = 0.10; r = 0.39). There was no relationship between SCR or BL rate classifications and DAG1 (P ≥ 0.66), SERPINA5 (P ≥ 0.54), or percentage of sperm tail labeled for SERPINA5 (P ≥ 0.48).

Proteomic Analysis Of Epididymal And Ejaculated Sperm And Respective Fluids, Saulo Menegatti Zoca, Emmalee J. Northrop-Albrecht, Julie Walker, Robert A. Cushman, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Ejaculated and epididymal semen was collected from mature Angus bulls (n = 9), and then centrifuged to separate sperm and fluid. Fluids were collected and sperm pellets were resuspended in a high ionic solution and vortexed to remove loosely attached proteins. Sperm samples were centrifuged, and the supernatant was collected. Samples collected for protein analysis were snap frozen in liquid nitrogen and stored at -80 ºC. Protein analysis was performed by liquid chromatography with tandem mass spectrometry (LCMS/MS). Yearling Angus cross bulls (n = 40) were used for sperm cultures. Ejaculated (n = 20) and epididymal (n = …

Effect Of Estrous Synchronization With Natural Service Or Fixed-Timed Artificial Insemination Using Conventional Or Gender-Kkewed Semen In Beef Females On Calving Distribution And Post Weaning Calf Performance, Julie Walker, Jerica J.J. Rich, Warren Rusche, Matthew Diersen, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Within 10 herds, beef females (n = 1,620) were either: 1) not synchronized (NonSyn) and mated to bulls, 2) synchronized (7-d controlled internal drug release (CIDR)) and mated to bulls (SynNS) 3) synchronized (7-d CO-Synch plus CIDR) and artificially inseminated with conventional semen (SynAI), or 4) synchronized (7-d CO-Synch plus CIDR) and artificially inseminated with SEXED semen. Calving distributions (calves born from d 1 to 14, 1 to 21, 22 to 42, and 43 and greater) were determined by actual birthdates and calf gender was determined at birth. Over a two-year period, a subset of calves (n = …

Improving Heifer Development Programs Using Precision Technology And Ddgs, Anna Dagel, Hector Menendez Iii, Jameson R. Brennan

SDSU Beef Day 2022 Summary Publication

Study Description:

Heifers were stratified into two groups based on initial body weight and each group was randomly assigned to one of two treatments: a control group (n=30) and a precision group (n=30). Treatment groups are separated into different pastures to graze dormant native range from November to April. Groups are rotated monthly to reduce individual pasture influence. Heifers in the control group are supplemented as a group and bunk fed 5lb/head/day with dried distiller grain pellets (DDGS). Heifers within the precision group will be supplemented individually using the SmartFeed system (C-Lock Inc. Rapid City, SD) and offered 5lb/head/day DDGS …

Factors Influencing Price Of South Dakota Feeder Calves Fall 2021 And Winter 2022, Adele Harty, Olivia Amundson, Robin Salverson, Kiernan Brandt, Heather Gessner, Julie Walker, Hossein Moradi Rekabdarkolaee

SDSU Beef Day 2022 Summary Publication

Study Description:

Data was collected at Faith, Philip, Hub City (Aberdeen), Mitchell, and Ft. Pierre Livestock Auctions during the weeks of October 4, 11, 18, and November 1, 2021. Data was collected and analyzed on the approximately 116,000 head of calves that were marketed during that time frame. Data collected included: auction barn, date, seller id, sex, number of head per lot, average weight, hide color, uniformity information, vaccination data, creep feed, implants, branded programs, weaned, horns, mud score, price per hundredweight, price per head, and additional comments. Uniformity score and weighted average price per hundredweight was calculated for each …

Comparing The Impact Of Continuous And Virtually Fenced Rotational Grazing On Animal Behavior And Distribution, Logan Vandermark, Jameson Brennan, Krista Ehlert, Hector Menendez Iii

SDSU Beef Day 2022 Summary Publication

Study Description:

Six herds of yearling steers (n=127) will graze native summer pastures from May to August at the Cottonwood Field Station near Philip, South Dakota. Six pastures are divided into two groups: a continuous grazing (CG) treatment with a low, moderate, and high stocking density and a virtually fenced rotational (VFR) grazing system with the same stocking densities. VenceTM virtual fence collars were placed on all animals within the study and collected GPS data at 5-minute intervals. The CG collars are not actively managed. The VFR system is actively managed through the collars and rotated based on the amount …

Using Precision Technology To Measure Cattle Methane Emissions And Intake On Western South Dakota Rangelands, Lillian J. Mcfadden, Ken Olson, Krista Ehlert, Jameson Brennan, Amanda D. Blair, Hector Menendez Iii

SDSU Beef Day 2022 Summary Publication

Study Description:

Cattle DMI is an essential component of calculating cattle stocking rates, determining nutrient requirements, and evaluating feed efficiency. Cattle DMI and digestion of forages impact enteric greenhouse gas (GHG) emissions; a major public and environmental concern. Increased GHG levels indicate energy loss during the rumen fermentation process. Obtaining data for rangeland cattle DMI and GHG emissions is needed to understand and enhance individual animal performance and reduce negative environmental impacts. We will develop enteric emissions and DMI relationships by conducting three feeding trials using the GreenFeed and SmartFeed Pro (C-Lock Inc. Rapid City, SD). The GreenFeed will measure …

Effects Of Environmental Gradients On The Abundance And Diversity Of Helminths Across 7 Bison Herds In The Upper Midwest, Bradly Wehus-Tow, Jeff M. Martin, Jameson Brennan

SDSU Beef Day 2022 Summary Publication

Study Description:

Data were collected from Wind Cave National Park and 6 satellite bison herds across the upper Midwest ranging across South Dakota, Indiana, and Kansas with a MAP gradient ranging from 34 to 82 mm and a MAT gradient ranging from 9 to 13°C. Fecal samples were collected non-invasively from 50 freshly voided bison dung patties at each herd. Helminth eggs were recovered and identified using sugar floatation; Trichostrongyle-type eggs were hatched by coproculture to identify to species. Preliminary findings indicate that general abundance of trichostrongyle-type helminths increases by 2.3 EPG per 1°C and by 0.2 EPG per mm, …

Influence Of Beef Carcass Chilling Rate On Steak Case Life And Quality Traits, Trevor Dehaan, Lydia Hite, Erin Gubbels, Christina Elaine Bakker, Amanda D. Blair, Judson Grubbs, Keith Underwood

SDSU Beef Day 2022 Summary Publication

Study Description:

Twelve head of fed beef cattle were harvested at the SDSU Meat Laboratory over two days. Carcasses were allotted into two weight groups based on hot carcass weight (HCW): Heavyweight (HW) and Lightweight (LW). Data logging thermometers were placed in the left side of each carcass within the round, loin, rib, and chuck primals to track temperature decline. Carcass measurements including 12th rib fat thickness, ribeye area and marbling score were collected approximately 48 hours postmortem. Steaks from each primal were collected to measure Warner-Bratzler shear force (WBSF), objective and subjective color.

Serving Beef In Usda Child Nutrition Programs In South Dakota: Development Of An Faq, Anna Barr, Mikayla Hardy, Andrea Alma, Heather Hopwood, Joanna Studt

SDSU Beef Day 2022 Summary Publication

Study Description:

The project occurred in partnership with four organizations representing state, tribal, and federal affiliations. The authors collaborated to compile and answer questions. Questions that were not answered by the authors’ organizations were asked to the SD Animal Industry Board and USDA Food Safety and Inspection Service.

Comparison Of Phosphorus Removal And Recovery Methods And Proposed Design Of High Rate Algal Pond For Swine Wastewater, Taylor Fauth

Civil and Environmental Engineering Graduate Students Plan B Capstone Projects

High phosphorus concentrations in wastewater effluent can have harmful effects to aquatic environments and water quality. An excess of nutrients such as phosphorus in water bodies will cause eutrophication, which depletes the available oxygen within the water and is detrimental to aquatic life. Additionally, phosphorus leaching from wastewater discharge has negative impacts to groundwater quality. The depletion of phosphorus as a natural resource is also of concern as phosphorus is an essential product for agricultural and industrial production. Phosphorus is a non-renewable resource, and natural sources are becoming more difficult and expensive to access. Therefore, the removal and recycle of …

Characterizing Mouse Line-1 5’Utr Antisense Promoter Activities, Jada Nicole Tschetter

Schultz-Werth Award Papers

Mice are often the model of choice for genomic research because of their high similarity to the human genome and relatively easy maintenance. Thus, understanding the mechanisms and principles of their genome is vital to advancing our knowledge about human biology. Long interspersed elements type 1 (LINE-1s or L1s) are transposable elements within mammalian DNA. Promoter activities have been identified for the human L1 5’ untranslated region (5’UTR) in both the sense and antisense orientation, but it was previously unknown if mouse L1 5’UTRs have antisense promoter activity. The goal of my project was to uncover mouse L1 5’UTR promoter …

Non-Invasive Biomarkers For The Diagnosis Of Endometriosis And Polycystic Ovary Syndrome, Chandana Kamaraj

Biology and Microbiology Graduate Students Plan B Research Projects

Benign gynecological disorders can affect a high percentage of women of reproductive age, ages 15-44 years. These conditions can affect the lifestyle of the individual and can be associated with infertility. The gold standard to identify and diagnose endometriosis requires invasive surgical procedures, while the Rotterdam Criteria is used to identify and diagnose polycystic ovary syndrome. The purpose of this paper is to discuss, describe, and characterize potential non-invasive biomarkers that are present in various pathological stages of both endometriosis and polycystic ovary syndrome. These biomarkers include CA125, Serum galectin-9, hsa-miRNA-154-5p, miRNA-93, miRNA-320 and ET-1, miRNA-222, miRNA-146a, and miR-30c used …

Dairy Digest 2022 : A Hoofprint In Time, South Dakota State University Dairy Club

Dairy Digest

This is the 2022 Dairy Digest. The Diary Digest is published annually by the South Dakota State University Dairy Club. The Digest contains information and news about the activities and members of the SDSU Dairy Club for the academic year 2021 - 2022.

School Of Health And Consumer Sciences Free Communication Day: Research Abstracts, Spring 2022., Mary Beth Zwart, Elizabeth Droke, Hungling (Stella) Liu

Health and Nutritional Sciences Graduate Students Plan B Capstone Projects

This document contains Plan B abstracts presented by graduate students at the School of Health and Nutritional Sciences Free Communication Day: Research Dissemination held on May 3rd, 2022.

Project titles and presenter included:

• Development of Balanced Budget Bites and Feasibility Evaluation
  (Holly Hansen)
  
  
• The Effectiveness of Extracorporeal Shockwave Therapy on Reducing Pain and Improving Functionality in Athletes with Patellar Tendinopathy
  (Maranna Gunnerson)
  
  
• Community Perception of Small Town Outdoor Aquatic Facility: Case of the Huether Family Aquatic Center.
  (Logan Haak)
  
  
• The Gut Microbiome Profile of the American Indian and Alaska Native Population: A Narrative Review
  (Blair Groebner)
  
  
• The Prevalence of Myocarditis …

South Dakota State University Undergraduate Catalog 2022-2023, South Dakota State University

South Dakota State University Catalogs and Bulletins: 1935 to present

No abstract provided.

Evaluation Of The Effects Of Water Quality On Drinking Preferences Of Heifer Calves, Nirosh Senevirathne, Jill Anderson

Dairy Science and Food Science Faculty Publications: 2022 to Present

Drinking water quality requirements focus mainly on removing hazardous substances from the water supply, making it safe to drink and appropriate for livestock use. However, there is a concern that high mineral content in water may affect the drinking behavior of heifer calves, which may affect health and performance. In South Dakota, the primary water sources for agricultural use are surface or groundwater. Water quality varies between different regions of the state; in the eastern part of the state, the water is classified as “hard water” and calcium is the primary mineral found in the water. Our objective was to …

South Dakota State University Graduate Catalog 2022-2023, South Dakota State University

South Dakota State University Catalogs and Bulletins: 1935 to present

No abstract provided.

Regenerative Agriculture Effects On Invertebrate And Bird Communities And Insect-Provided Ecosystem Services, Alex Michels

Electronic Theses and Dissertations

Conventional agricultural practices can have unintended consequences on the environment and wildlife. Insects and birds are declining at rapid rates around the world, and the current conventional agricultural paradigm is a major driver through habitat loss and the intensification of production. Invertebrates in agroecosystems provide services to both farmers and the rest of society. Regenerative systems may promote the functioning of an agroecosystem by influencing invertebrate abundance, diversity, and ecosystem services and mitigate bird and insect declines through conservation practices that increase soil health, reduce disturbances, and increase biological diversity. Here I address knowledge gaps of the effects of regenerative …

Response Of Soil Biochemical And Physical Properties To Long Term Prairie Cordgrass And Kura Clover Intercropping System, Vaishnavi Varikuti

Electronic Theses and Dissertations

Prairie cordgrass (Spartina pectinata) (PCG) is a warm-season perennial grass that can be used as a biofuel feedstock and can be grown on marginal lands. Previous studies on intercropping of a perennial legume i.e., kura clover (Trifolium ambiguum) (KC) with PCG can improve soil biochemical properties, increase biomass production, mitigate greenhouse gas (GHG) emissions while reducing the chemical fertilizer requirement. However, there is a lack of evidence about the effects of PCG production on soil biochemical and physical properties during the cropping season and at deeper soil depths in reference to support plant growth, environmental implications and enhance the soil …

Eastern Redcedar (Juniperus Virginiana L.) Encroachment On South-Central South Dakota Rangelands: Impact On Plant Communities, Austin K. Domeier

Electronic Theses and Dissertations

Grasslands historically covered 46 million km2 of Earth’s surface representing nearly 42% of the living vegetation. Encroachment of woody species onto grasslands and savannas is a widely researched global phenomenon, with eastern redcedar (ERC) (Juniperus virginiana L.) trees being the most prominent woody encroacher in the Northern Great Plains (NGP) due to the lack of prescribed fire and planted ERC escaping from shelterbelts. This encroachment poses a threat to native plant communities in terms of their reproduction, regeneration, and diversity. ERC are fierce competitors and can establish in most soil types, are drought tolerant, and prolific seed reproducers. These qualities …

Design, Development, And Testing Of Near-Optimal Satellite Attitude Control Strategies, Giovanni Lavezzi

Electronic Theses and Dissertations

Advances in space technology and interest toward remote sensing mission have grown in the recent years, requiring the attitude control subsystems of observation satellites to increase their performances in terms of pointing accuracy and on-board implementability. Moreover, an increased interest in small satellite missions and the recent technological developments related to the CubeSats standard have drastically reduced the cost of producing and flying a satellite mission. In this context, the proposed research aims to improve the state of the art for satellite attitude control methodologies by proposing a near-optimal attitude control strategy, simulated in a high-fidelity environment. Two strategies are …

Effect Of Finishing System And Animal Age On Carcass Traits And Nutritional Profile Of Bison Bulls, Clay Newton

Electronic Theses and Dissertations

The objectives of this thesis project were to 1) evaluate the influence of grain- and grass-finishing systems on carcass characteristics, proximate composition, nutritional profile, and fatty acid composition of bison bull meat, and 2) evaluate the influence of animal age on carcass characteristics of bison bulls. For objective 1, bison bulls were allowed to graze native range in north-central Nebraska until approximately 26 mo of age, when they were randomly assigned to either grain-finishing (n = 98; in an open lot with ad libitum access to prairie hay, alfalfa hay and corn for 95 d prior to slaughter) or grass-finishing …

Living In A Gradient: The Influence Of Water Temperature Variation On Development, Settling Time And Survival Of Pallid Sturgeon Larvae In The Missouri River, Maria Elizabeth Erceg

Electronic Theses and Dissertations

Pallid Sturgeon (Scaphirhynchus albus) are a federally endangered species experiencing widespread reproduction and recruitment failures. Recruitment failure is hypothesized to be caused by habitat modifications made to the Missouri River that disrupt connectivity and alter temperature profiles downstream of constructed dams. Hypolimnetic releases from Missouri River dams affect the temperature downstream, creating colder conditions during the downstream drifting phase for dispersing larvae. Understanding the influence of water temperature changes on larval development is crucial for recovery efforts. In this study, we evaluated the effects of water temperature daily heating rate on energy use, settling behavior, and growth rate of endogenously …