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Monarch Butterfly (Danaus Plexippus) Oviposition Preferences And A Survey Of Fungal Pathogens On Native Milkweeds (Asclepias) Of The Northern Great Plains, Grace Damiano Jan 2024

Monarch Butterfly (Danaus Plexippus) Oviposition Preferences And A Survey Of Fungal Pathogens On Native Milkweeds (Asclepias) Of The Northern Great Plains, Grace Damiano

Electronic Theses and Dissertations

North American monarch butterfly (Danaus plexippus) populations are experiencing significant declines due to habitat loss caused by land use changes. Much of the American Midwest where milkweed, the only host plant of the monarch butterfly, was once much more abundant has been converted to a predominantly agricultural landscape. There are nineteen species of milkweed native to the northern Great Plains region occupied by South Dakota, North Dakota, and Minnesota. Some of these milkweed species may be preferable to monarch butterflies for different reasons, including morphological factors such as stem length, species-level chemical factors, and temporal factors. As monarch …


Evaluation Of Dietary Fiber Technologies In Sow Diets, Garrin Shipman Jan 2024

Evaluation Of Dietary Fiber Technologies In Sow Diets, Garrin Shipman

Electronic Theses and Dissertations

Various technologies and alternative ingredients are being developed to increase the nutrient value of fibrous feedstuffs, improve animal performance, and promote sustainable swine production. Fiber-degrading enzymes have been extensively studied in growing pigs, with few studies focused on gestating sows. When considering gestating sows have greater energy digestibility of fibrous feedstuffs than grower pigs, the efficacy of fiber-degrading enzymes needs to be determined for implementation in commercial sow diets. As fiber inclusion in gestation diets benefits sow performance, corn dried distiller grains with solubles (DDGS) are a candidate fiber source for gestation alternative energy ingredient. Functional fibers, such as yeast …


Effects Of Isoacids Supplementation In Lactating Cows’ Diet Varying In Forage Fiber Level On Performances, Feed Efficiency, And Milk Fatty Acids Profile, Sumon Ahmed Jan 2024

Effects Of Isoacids Supplementation In Lactating Cows’ Diet Varying In Forage Fiber Level On Performances, Feed Efficiency, And Milk Fatty Acids Profile, Sumon Ahmed

Electronic Theses and Dissertations

No abstract provided.


Evaluation Of Soybean Meal Versus Distillers Co-Products As A Protein Supplement For Finishing Beef Cattle: Effects On Growth Performance, Carcass Characteristics, And Efficiency Of Dietary Net Energy Utilization, Cassidy R. Ross Jan 2024

Evaluation Of Soybean Meal Versus Distillers Co-Products As A Protein Supplement For Finishing Beef Cattle: Effects On Growth Performance, Carcass Characteristics, And Efficiency Of Dietary Net Energy Utilization, Cassidy R. Ross

Electronic Theses and Dissertations

Two randomized complete block design experiments were conducted to: 1) examine the effects of soybean meal with or without additional soybean hulls in replacement of modified corn distillers grains plus (MDGS) solubles on growth performance efficiency of dietary net energy utilization and carcass traits responses in finishing beef steers, 2) determine if partial or complete replacement of dried distillers grains plus solubles (DDGS) with solvent-extracted soybean meal (SBM) in diets based upon high-moisture ensiled corn influences growth performance, efficiency of dietary net energy utilization, sera urea-N (SUN) concentrations, or carcass traits in finishing beef steers. Experiment 1 was a 118-d …


Removal Of Phosphorous, Copper And Zinc From Stormwater Using Powdered Activated Carbon (Pac) Based Water Treatment Residuals, Foysol Mahmud Jan 2024

Removal Of Phosphorous, Copper And Zinc From Stormwater Using Powdered Activated Carbon (Pac) Based Water Treatment Residuals, Foysol Mahmud

Electronic Theses and Dissertations

The main aim of this research was to evaluate the efficacy of Powdered Activated Carbon (PAC) based water treatment residual (WTR) in eliminating phosphorus (P), Copper (Cu), and Zinc (Zn) from stormwater. The characteristics of the PAC WTR were analyzed, and a series of batch and column tests were conducted to determine its capacity to remove P, Cu, and Zn from stormwater and synthetic solutions. The PAC WTR conformed to the Langmuir isotherm model, demonstrating P adsorption capacities of 3.51 mg/g at pH 5. Regarding Cu and Zn removal, the PAC WTR also adhered to the Langmuir isotherm model, showing …


Estimating South Dakota’S Capacity To Meet K-12 School Meal Fruit And Vegetable Requirements With State-Grown Produce, Bridgette Bienias Jan 2024

Estimating South Dakota’S Capacity To Meet K-12 School Meal Fruit And Vegetable Requirements With State-Grown Produce, Bridgette Bienias

Electronic Theses and Dissertations

Objective: Quantify South Dakota (SD)’s capacity to procure locally-sourced fruit and vegetables (FVs) for K-12 school meals.
Methods: Secondary analysis of 2017 SD agricultural census and school meal data. Yields were converted to edible cups of FVs using school meal guidelines provided by the U.S. Department of Agriculture. Student enrollment and meal redemptions were used to estimate the number of FV cups needed.
Results: Estimated SD FV production met minimum total K-12 cup requirements, except for total fruit (66%) and dark green vegetables (80%). Legumes supplied 77% of the total cups of vegetables produced in 2017, whereas dark green vegetables …


Abelson (Abl) Tyrosine Kinase Inhibitors Reduce Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Replication In Vitro, Jenna Parliament Jan 2024

Abelson (Abl) Tyrosine Kinase Inhibitors Reduce Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Replication In Vitro, Jenna Parliament

Electronic Theses and Dissertations

Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped, positive-sense, single-stranded RNA virus from the Arteriviridae family. PRRSV causes respiratory illness and reproductive failure in infected pigs. Outbreaks occurring in the United States and worldwide cause significant economic losses annually. Abelson (Abl) tyrosine kinase is a non-receptor tyrosine kinase, which plays a central role in regulating many cellular processes such as actin reorganization, cell proliferation and differentiation by transducing extracellular and intracellular signals. Abl inhibitors have been shown to restrict the entry of membrane fusion of enveloped viruses, such as severe acute respiratory syndrome coronavirus, Middle Eastern respiratory syndrome …


Evaluation Of Nutrient Removal From Modified Woodchips And Organic Matter Leached From Tree Leaves After Simulated Wildfire Thermal Alteration, Zengyue Wang Jan 2024

Evaluation Of Nutrient Removal From Modified Woodchips And Organic Matter Leached From Tree Leaves After Simulated Wildfire Thermal Alteration, Zengyue Wang

Electronic Theses and Dissertations

Nonpoint source pollution (NPS) from stormwater runoff, carrying a variety of contaminants, presents substantial challenges to water treatment processes and public health. Among the primary pollutants, phosphate and nitrate significantly contribute to the degradation of water quality, resulting in issues such as eutrophication and methemoglobinemia. Tree leaves, which are a major source of dissolved organic carbon (DOC) in forested watersheds and serve as a key fuel for wildfires, further exacerbate these challenges. Post-wildfire, the altered DOC is mobilized by precipitation, leading to stormwater runoff that impacts natural water bodies and complicates treatment processes, particularly through the formation of hazardous disinfection …


Nitrogen Fertilizer Placement And Rate Impacts Sunflower Seed Yield, Oil And Protein Content, Rojina Kafle Jan 2024

Nitrogen Fertilizer Placement And Rate Impacts Sunflower Seed Yield, Oil And Protein Content, Rojina Kafle

Electronic Theses and Dissertations

South Dakota accounts for about 36% of the total US commercial sunflower production and nitrogen is a major input. Costs related to N fertilizer demand economically optimum N rates. This study investigated the impact of the N fertilizer placement method (broadcast vs band) and rate (0, 28, 56, 84, 112, 140 kg N ha-1) on leaf chlorophyll content, head diameter, seed yield, seed oil, and protein concentration of three sunflower hybrids in 2022 and 2023. The experiment was conducted at Brookings (eastern SD- conventional till, 66 cm rainfall) using an oil-type hybrid and at central SD sites (no-till, 57 cm …


Reimagining Hunting Through Women's Decision Space, H. Nash Jackson Jan 2024

Reimagining Hunting Through Women's Decision Space, H. Nash Jackson

Electronic Theses and Dissertations

Approximately 14.4 million people hunt in the United States, yet only 3.1 million (22%) of these hunters are women. To enhance women’s participation, many state natural resource agencies aim to reduce barriers and create pathways for women to hunt. Previously, researchers surveyed women to understand and describe barriers to their full participation in hunting. However, this methodology often just quantifies the different barriers without describing the interconnectedness and how the complexity of barriers can impact women’s participation in hunting. This thesis instead took an inductive research approach with the goal of better understanding women’s perspectives when faced with complex barriers. …


Increasing Soy Products Inclusion In Swine Diets To Improve Growth Performance, Health, And Pork Quality, Ashir Foreman Atoo Jan 2024

Increasing Soy Products Inclusion In Swine Diets To Improve Growth Performance, Health, And Pork Quality, Ashir Foreman Atoo

Electronic Theses and Dissertations

Despite its excellent nutritional profile, soybean meal (SBM) is limited in nursery pig diets due to its anti-nutritional factors (ANFs), which young pigs tolerate poorly. In the first study, strategies to mitigate these effects included supplementation with enzymes, alone or with acidifiers. A total of 240 weaned pigs (5.9 ± 0.8 kg average body weight [BW]) were assigned in randomized complete block design to five dietary treatments: low, medium, or high SBM diets, high SBM with multienzyme, and high SBM with multienzyme plus acidifier. Diets were fed in three phases: phase 1 (0–1 weeks), phase 2 (1–3 weeks), and phase …


Translocations Of North American Bison Across Large Climatic Gradients: Impacts On Thermoregulation, Body Size, And Helmintic Infection, Hayden Wolfe Jan 2024

Translocations Of North American Bison Across Large Climatic Gradients: Impacts On Thermoregulation, Body Size, And Helmintic Infection, Hayden Wolfe

Electronic Theses and Dissertations

Every year, one-eighth of all North American bison (Bison bison) are translocated, often across large climatic gradients. Over the last 16 years bison have been translocated from Wind Cave National Park in South Dakota to establish six herds across the United States central grasslands in Iowa, Missouri, Illinois, Indiana, and Kansas. Influence of climatic gradients on herd-level thermoregulation, body size, and helmintic infection were evaluated. Overall, 1174 thermal images and 1050 fecal samples were collected between 2021 and 2023. Physiological heat flux was used to measure thermoregulation, asymptotic body mass (ABM) was used for body size, and helminth genera abundance …


Beef Hot Carcass Weight Influences Proteolysis, Clay J. Carlson Jan 2024

Beef Hot Carcass Weight Influences Proteolysis, Clay J. Carlson

Electronic Theses and Dissertations

The objective of this thesis was to determine the influence of beef hot carcass weight on proteolysis to better understand early postmortem proteolysis. Beef tenderness formation is influenced by various factors, such as temperature decline and proteolysis. Limited focus has been given to the effects of carcass weight on proteolysis. This study aimed to investigate the influence of increased beef hot carcass weight (HCW) on proteolysis of four muscles (serratus ventralis, longissimus thoracis, longissimus lumborum, and semitendinosus) during early postmortem aging times. Beef steers (n =12) were harvested and categorized into two weight groups: lightweight (LW; HCW = 349 ± …


Improving Stream Connectivity In South Dakota, Colton Rainier Curtis Jan 2024

Improving Stream Connectivity In South Dakota, Colton Rainier Curtis

Electronic Theses and Dissertations

Stream connectivity is crucial for fish movement and genetic diversity in fragmented landscapes. Road-crossings often act as barriers, disrupting hydrology and isolating fish populations. The Southeast Aquatic Resources Partnership (SARP) has developed rapid road-crossing assessment protocols and tools to prioritize restoration projects. However, managers lack tools for planning, directing, and initiating roadcrossing assessments. Managers also require modeling approaches to effectively identify and monitor road-crossings across dynamic stream networks. Presented here is a case study offering a roadmap for effective implementation of collaborative road-crossing assessments and prioritization of remediation projects, alongside an exploration of predictive modeling approaches to identify problematic crossings …


2,3,4 Trihydroxybenzoic Acid, A Plant Secondary Metabolite Inhibits Cancer Cell Growth, Mina Akter Jan 2024

2,3,4 Trihydroxybenzoic Acid, A Plant Secondary Metabolite Inhibits Cancer Cell Growth, Mina Akter

Electronic Theses and Dissertations

Nature offers many sources such as fruits, beverage crops, vegetables, herbs, spices, tea, dark chocolate, and wine, that are rich in hydroxybenzoic acids (HBAs). They are a prominent phenolic acids with excellent biochemical and antioxidant properties. Due to the vast dietary health benefits of HBAs such as anti-inflammatory, anti-allergenic, immunoregulatory, antimicrobial, antithrombotic, antidiabetic, anticancer properties and cardioprotective capabilities, they are attracting the attention of physicians and researchers alike to understand their mechanisms of action. It is anticipated that the understanding of the role of HBAs for their greater use in diet/supplements is beneficial to reverse common illnesses such as inflammation, …


Investigating The Role And Mechanism Of Host Cell Death In Porcine Reproductive And Respiratory Virus Infection, Michelle Steyn Jan 2024

Investigating The Role And Mechanism Of Host Cell Death In Porcine Reproductive And Respiratory Virus Infection, Michelle Steyn

Electronic Theses and Dissertations

No abstract provided.


Organizational Culture's Impact On Job Satisfaction: A Reconceptualization Of The Competing Values Framework, Isaac Thor Erickson Jan 2024

Organizational Culture's Impact On Job Satisfaction: A Reconceptualization Of The Competing Values Framework, Isaac Thor Erickson

Electronic Theses and Dissertations

Academic job satisfaction has been decreasing in the U.S. which has profound negative impacts such as burnout and decreased well-being of employees. One way that job satisfaction can be increased is through understanding and changing organizational cultures through better communication. The Competing Values Framework (CVF; Quinn & Rohrbaugh, 1983; Belasen & Frank, 2010; Lund, 2003) has been used to understand how organizational cultures and their communicative norms relate to job satisfaction. However, the CVF relies on categorical measures sitting on two axes that are not orthogonal. As such, the study posits breaking these two axes into four dimensions of organizational …


The Spatial Ecology Of Plains Spotted Skunks In South Dakota: Insights From Species Distribution, Resource Selection, And Co-Occurrence Models, Kara M. White Jan 2024

The Spatial Ecology Of Plains Spotted Skunks In South Dakota: Insights From Species Distribution, Resource Selection, And Co-Occurrence Models, Kara M. White

Electronic Theses and Dissertations

The plains spotted skunk (Spilogale interrupta) is a data-deficient small carnivore native to the central plains of North America that has experienced significant population declines. My dissertation investigates the species’ distribution, habitat associations, and interspecific interactions in South Dakota, providing crucial information for conservation actions. In Chapter 1, I developed ensemble distribution models on a broad scale, incorporating species location data from 1985–2020, environmental factors, and six predictive modeling algorithms. I identified key predictors including mean temperature diurnal range and proportion of area as pasture, and estimated ~31,300 km2 of potential habitat, predominately in eastern South Dakota. In Chapter 2, …


Delivery Of Gemcitabine, Stearoyl Gemcitabine And Paclitaxel Through Plga Nanoparticles And Its Effect On The Desmoplasia Of Pancreatic Cancer Spheroids, Rishabh Bahl Jan 2024

Delivery Of Gemcitabine, Stearoyl Gemcitabine And Paclitaxel Through Plga Nanoparticles And Its Effect On The Desmoplasia Of Pancreatic Cancer Spheroids, Rishabh Bahl

Electronic Theses and Dissertations

Pancreatic cancer is one of the deadliest cancers to treat. The primary cause for the drug resistance is the dense extracellular matrix (ECM) that surrounds the tumor and restricts blood supply. Gemcitabine (GEM) and paclitaxel (PTX) are drugs that are clinically used in the treatment of pancreatic cancer. GEM, however, is rapidly metabolized by cytidine deaminase (CDA) and eliminated from the body, and paclitaxel is a substrate for P- glycoprotein (P-gp) efflux receptors. Drug delivery using nanoparticles has been reported to help improve drug half-lives and bypass efflux receptors. This thesis aims to encapsulate PTX in PLGA nanoparticles (PTX-NP) and …


Longevity In Afrikaner Cattle: A Survival Analysis, Allison M. Bot Steffl Jan 2024

Longevity In Afrikaner Cattle: A Survival Analysis, Allison M. Bot Steffl

Electronic Theses and Dissertations

The Afrikaner breed of cattle is indigenous to South Africa and due to their hardiness was once the most popular breed amongst farmers. The objectives for this study were to: (1) estimate environmental effects (age at first calving, season) and (2) genetic variance affecting longevity in Afrikaner cattle using survival analysis and a Weibull model and (3) evaluate the effect of the Infusion Project on longevity utilizing a Cox model. For the first objective, age at first calving impacted longevity as younger age at first calving was associated with decreased longevity. Season of birth and year of birth also impacted …


Methods For Optimizing Detection And Evaluating Habitat Use Of North American River Otters In South Dakota, Jessica Speiser Jan 2024

Methods For Optimizing Detection And Evaluating Habitat Use Of North American River Otters In South Dakota, Jessica Speiser

Electronic Theses and Dissertations

North American river otters (Lontra canadensis) play a critical role as an apex predator in riverine ecosystems. A challenging species to survey using traditional methods, they remain understudied in non-forested regions, such as the Northern Great Plains, where habitat degradation and landscape fragmentation may reduce habitat quality and availability and disrupt landscape connectivity. This thesis aims to improve detectability and investigates the habitat use of river otters in South Dakota to better understand habitat needs of otters and improve their continued recovery and management. My first chapter provides a brief overview of river otters and their ecological roles in freshwater …


Respiratory Disease In Bighorn Sheep: A Varied Investigation Of Immunization, Transmission, And Detection, Katelin Frerichs Jan 2024

Respiratory Disease In Bighorn Sheep: A Varied Investigation Of Immunization, Transmission, And Detection, Katelin Frerichs

Electronic Theses and Dissertations

Respiratory disease has historically been recognized as a limiting factor to wild bighorn sheep (Ovis canadensis) herds throughout North America with epizootics resulting in large-scale die offs and reductions in recruitment. The primary etiologic agent associated with pneumonia outbreaks in bighorn sheep is Mycoplasma ovipneumoniae. This bacterial pathogen is frequently carried asymptomatically by domestic sheep (Ovis aries) and goats (Capra hircus); however, when these domestic hosts come in contact with and spread this pathogen to bighorns, the disease can cause epizootics and large-scale mortality. Domestic sheep strains are more commonly detected in bighorn sheep than domestic goat strains, and previous …


Isolation, Molecular Characterization, And Epidemiological Analysis Of An Emerging Avian Metapneumovirus Subgroup B In Us Poultry, Sumit Jangra Jan 2024

Isolation, Molecular Characterization, And Epidemiological Analysis Of An Emerging Avian Metapneumovirus Subgroup B In Us Poultry, Sumit Jangra

Electronic Theses and Dissertations

Avian metapneumovirus (aMPV) is one the most economically important poultry respiratory pathogens belonging to the family Pneumoviridae and genus Metapneumovirus, which can affect both domestic and wild birds. aMPVs are classified into four subgroups (A, B, C, and D) based on the level of genetic variations and antigenic differences. aMPV causes turkey rhinotracheitis (TRT) and swollen head syndrome (SHS) in turkeys and chickens respectively. Recently, outbreaks of respiratory disease in meat-type birds and a significant decline in egg production with respiratory signs in breeders were reported from different states in the USA. Isolating aMPV is challenging as it is a …


Evaluation Of A Novel Corn Fermented Protein Ingredient, Probiotics, And Postbiotics On Nursery Pig Growth Performance And Gut Health, Juan F. Castillo Zuniga Jan 2024

Evaluation Of A Novel Corn Fermented Protein Ingredient, Probiotics, And Postbiotics On Nursery Pig Growth Performance And Gut Health, Juan F. Castillo Zuniga

Electronic Theses and Dissertations

Weaning is a necessary step in pig production. After weaning, pigs are subjected to various nutritional, psychological, and environmental stresses. The weaning period is an important period for the intestinal development of the pig. During this time, digestion, immunity, metabolism, and various other aspects of the pig change rapidly. Therefore, providing high-quality feed ingredients is crucial to encourage the newly weaned pig to start consuming solid feed. Two independent studies were conducted to determine if the inclusion of: 1) corn fermented protein (CFP) or 2) probiotics or postbiotics impact nursery pigs' growth performance and gut health. The first experiment utilized …


Field Scale Evaluation Of A Flow-Through Filtration System With Mixed Media To Remove E. Coli From Stormwater, Ankur Debnath Jan 2024

Field Scale Evaluation Of A Flow-Through Filtration System With Mixed Media To Remove E. Coli From Stormwater, Ankur Debnath

Electronic Theses and Dissertations

The main goal of this research was to establish a field-scale stormwater filtration system which is flow-through and cost-effective in terms of construction and maintenance. The filter is constructed in a downstream drainage of a retention pond where the stormwater goes through the porous space of media so that it maintains low retention time with minimum disturbance in flow. The filter consists of two 20ft long sections with concrete channel and ripraps on the inlet & outlet side. 30% Steel chips and 70% steel slags in the size range of 6.00-12.5 mm have been used as media which has been …


The Relationship Between Soil Test Phosphorus And Soil Biology - A Natural Management Of Soil Phosphorus Through Conservation Practices, Clarence Frederick Winter Jan 2024

The Relationship Between Soil Test Phosphorus And Soil Biology - A Natural Management Of Soil Phosphorus Through Conservation Practices, Clarence Frederick Winter

Electronic Theses and Dissertations

Phosphorus (P) management is an ongoing challenge in modern agriculture. Due to the importance of P in agriculture, P fertilizer additions are made throughout the U.S. to avoid nutrient-depleted soil. However, the application of P is problematic, as an excess of P in an agricultural system elevates the risk of environmental degradation, and current synthetic P sources are a fleeting resource. Additionally, a majority of P found in agricultural soils are insoluble, in the form of secondary and primary P minerals, making this large pool of P unavailable to plants. Fortunately, soil fungi organisms, such as arbuscular mycorrhizal fungi (AMF) …


Identification And Characterization Of Three Novel Rumen Bacterial Species In Sheep Fed A Concentrate Diet, Reid Frederick Anema Jan 2024

Identification And Characterization Of Three Novel Rumen Bacterial Species In Sheep Fed A Concentrate Diet, Reid Frederick Anema

Electronic Theses and Dissertations

Sheep production in the United States is a popular venture for both small farmers and large enterprise owners, as there is a market for end products such as wool and meat. One major factor of raising sheep is nutrition as an adequate diet will allow an animal to reach its full genetic potential for growth. With that in mind, the composition of the diet must be formulated to benefit the animal. Ruminants have the ability to use a variety of plants that are often inedible for humans to create products that humans can consume. These varying feedstuffs, whether they include …


Anemia In Highly Prolific Sows: Implications On Piglet Outcomes, Farrowing Complications, And Longevity Considerations, Katlyn A. Mcclellan Jan 2024

Anemia In Highly Prolific Sows: Implications On Piglet Outcomes, Farrowing Complications, And Longevity Considerations, Katlyn A. Mcclellan

Electronic Theses and Dissertations

No abstract provided.


Ecophysiology Of Bradyrhizobium Diazoefficiens Usda 110, Armaan Kaur Sandhu Jan 2024

Ecophysiology Of Bradyrhizobium Diazoefficiens Usda 110, Armaan Kaur Sandhu

Electronic Theses and Dissertations

With increasing demand for soybean, maximizing plant yield is important yiel is dependent on nitrogen availability, but nitrogen fertilizers can meet the plant's needs but have severe environmental impacts. An economically and environmentally sustainable approach is to enhance nitrogen availability through Biological Nitrogen Fixation by symbiotic rhizobia, Bradyrhizobium, an efficient nitrogen fixer for soybean. This symbiosis begins with attachment of rhizobia to legume roots. Root and rhizobia surface properties play a significant role in this attachment. Another major factor affecting root colonization is bacterial competition ability. There is a lack of knowledge on the role of bacterial surface properties, competition …


Role Of Interferon Induced Transmembrane Protein 3 (Ifitm3) And Neural Precursor Cell Expressed Developmentally Down Regulated Protein 4 (Nedd4) In Seneca Virus A (Sva) Replication, Shamiq Aftab Jan 2024

Role Of Interferon Induced Transmembrane Protein 3 (Ifitm3) And Neural Precursor Cell Expressed Developmentally Down Regulated Protein 4 (Nedd4) In Seneca Virus A (Sva) Replication, Shamiq Aftab

Electronic Theses and Dissertations

In this study, we studied the role of IFITM3 in SVA replication in vitro by silencing the endogenous IFITM3 in NCI-H1299 cells. An average of 1.94-fold increase in SVA VP2 expression and an average of 4.4-fold increase in supernatant virus titer was observed after 24h SVA isolate SD15-26 infection. We have indeed observed an increase in viral mRNA copies by an average of 5-fold in IFITM3 silenced NCI-H1299 cells at 8 hpi indicating that IFITM3 restricts SVA replication post virus attachment and entry. A positive correlation between interferon-alpha induced IFITM3 upregulation and reduced SVA replication was observed. To further confirm …