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The Effects Of A Blend Of Essential Oils On Rumen Efficiency Of Lactating Dairy Cows, Kali Linville Jan 2017

The Effects Of A Blend Of Essential Oils On Rumen Efficiency Of Lactating Dairy Cows, Kali Linville

Electronic Theses and Dissertations

The objective of this study was to evaluate the effects of SS a blend of commercially available essential oils (EO) on rumen microbial efficiency and kinetics, and consequently, effects on production parameters of dairy cows fed a low-starch diet and in vitro analysis of varying doses of SS on ruminal fermentation at different stages of lactation. The study consisted of two experiments, the in vivo Experiment 1, was conducted on a commercial robotic dairy in southwest Minnesota outfitted with two Lely Astronaut A4robotic milking units (Goter’s Clay & Dairy Equipment of MN, INC., Pipestone, MN). One hundred-seven Holstein cows were …

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Effect Of Ultrasonication On Biofilm Forming Ability Of Common Dairy Sporeformers, Taghreed Almalki Jan 2017

Effect Of Ultrasonication On Biofilm Forming Ability Of Common Dairy Sporeformers, Taghreed Almalki

Electronic Theses and Dissertations

Sporeformers are common dairy contaminants, which are of a great concern to the dairy industry. Quality of dairy products is affected by these sporeformers such as Bacillus species, which are a significant cause of spoilage of dairy products. Thermal processes such as pasteurization have been used to inactivate pathogens, but some of the sporeformers and their endospores are resistant to such heat treatments. Beside thermal treatments, various new approaches are being developed to improve the quality of dairy products. Amongst these, ultrasonication is a promising non-thermal technique for the inactivation of thermoduric sporeformers and their endospores. Current study was carried …

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Cardiovascular Risk, Remote Ischemic Postconditioning, And Endothelial Ischemia-Reperfusion Injury, Brian A. Hemenway Jan 2017

Cardiovascular Risk, Remote Ischemic Postconditioning, And Endothelial Ischemia-Reperfusion Injury, Brian A. Hemenway

Electronic Theses and Dissertations

Remote ischemic postconditioning (rIpost) is a potent vasculoprotective stimulus that is thought to reduce reperfusion injury associated with heart disease. However, certain animal models of cardiometabolic risk factors such as dyslipidemia, diabetes, and hypertension suggest the beneficial effects of rIpost to lessen reperfusion injury may be diminished. These findings have not been studied in clinically relevant humans. The aim of this study was to determine whether the protective effects of rIpost are reduced in the context of raised cardiometabolic risk in humans. Seven participants with raised cardiometabolic risk burden (age:57±7 years; BMI: 31±4kgm2participated in this 2X2 randomized cross-over …

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The Indirect Effect Of Bvdv On Immune Suppression: The Role Of Infected Macrophages In Lymphocyte Apoptosis, Karim Abdelsalam Jan 2017

The Indirect Effect Of Bvdv On Immune Suppression: The Role Of Infected Macrophages In Lymphocyte Apoptosis, Karim Abdelsalam

Electronic Theses and Dissertations

BVDV is an important pathogen of cattle that affects both the dairy and beef industry causing severe economic losses. The main problem of BVDV is the immune suppression that is associated with lymphoid depletion shortly post infection. The mechanism of this immune suppression is not well understood. In this in vitro study, we sought to determine the indirect effect of BVDV infection in macrophages as a key player in the pathogenesis of BVD especially, the apoptotic effect induced on lymphocytes. We infected bovine monocyte-derived macrophages (MDM) with either high or low virulent strains of cytopathogenic or non-cytopathogenic strains of BVDV …

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An Evaluation Of Deer And Pronghorn Surveys In South Dakota, Kristopher W. Cudmore Jan 2017

An Evaluation Of Deer And Pronghorn Surveys In South Dakota, Kristopher W. Cudmore

Electronic Theses and Dissertations

To properly manage white-tailed deer (Odocoileus virginianus), mule deer (Odocoileus hemionus), and the American pronghorn antelope (Antilocapridae americana), wildlife managers must identify population perimeters, sample size, age and sex ratios of these three species. The primary objective of this study was to evaluate age and sex ratios and determine a minimum sample size for each study area for all deer species and pronghorn, in addition to deriving a methodology and population estimate for white-tailed deer in the Black Hills. Sample size needed for both species of deer and pronghorn ranged from 60-70 groups of does. Age ratios calculated from daylight …

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Effects Of Phase Feeding During Gestation On Gilt Performance, Offspring Quality And Robustness, Agatha Ampaire Jan 2017

Effects Of Phase Feeding During Gestation On Gilt Performance, Offspring Quality And Robustness, Agatha Ampaire

Electronic Theses and Dissertations

A total of 51 gilts in 6 blocks were randomly assigned to one of 3 feeding regimens: Constant (Constant-f), 2.21 kg/d of a standard diet from breeding to d 112 of gestation (1.7 g Lys/kcal ME; 3276 kcal ME/kg); Bump feeding (Bump-f), the standard diet at 2.21 kg/d from breeding to d 89 and 2.61 kg/d from d 90 - 112; Phase feeding (Phase-f ), 2.21 kg/d from breeding to d 89 (1.5 g Lys/kcal ME; 3275 kcal ME/kg) and 2.61 kg/d from d 90 - 112 (2.1 g Lys/Kcal ME; 3290 Kcal ME/kg) over 2 reproductive cycles. Sows received …

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Understanding The Impacts Of Ammonia Fiber Expansion (Afex™) Pretreatment And Densification On Densified Products Quality And The Bioproducts Yield Through Enzymatic Hydrolysis And Fast Pyrolysis, Vijay Sundaram Jan 2017

Understanding The Impacts Of Ammonia Fiber Expansion (Afex™) Pretreatment And Densification On Densified Products Quality And The Bioproducts Yield Through Enzymatic Hydrolysis And Fast Pyrolysis, Vijay Sundaram

Electronic Theses and Dissertations

Lignocellulosic biomass poses significant challenges during handling, transportation, and storage due to its low bulk density. Densification involves conversion of the low bulk density biomass into a highly compacted product which helps in improving the handling, transporting, and storage obstacles associated with biomass logistics. Besides the logistical challenges, the recalcitrant nature of the lignocellulosic biomass makes it even more challenging during the enzymatic hydrolysis. The carbohydrate components, cellulose and hemicellulose are not readily accessible by the enzymes during the hydrolysis process due to the presence of lignin. Pretreatment is the process to convert the native recalcitrant biomass in the form, …

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Factors Affecting Feedlots' Decisions On Cattle Marketing Options: A National Study, Charlotte Owusu-Smart Jan 2017

Factors Affecting Feedlots' Decisions On Cattle Marketing Options: A National Study, Charlotte Owusu-Smart

Electronic Theses and Dissertations

This thesis investigates the economic implication of changing slaughter volume patterns across the cash and contract markets in the US cattle industry. Weekly time series data that spans from November 2002 to December 2015 was used in the analysis. Empirical evidence suggests that Mandatory Price Reporting (MPR) played an important role in changing slaughter volumes across the cash and contract markets. Additionally, price of feeder cattle, cattle quality characteristics (Choice-Select spread and the percentage of animals grading choice or better) among others influence feedlots’ decision to use a particular marketing arrangement.

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Comprehensive Structural, Thermal And Toxicological Characterization Of 1-Ethyl-3-Methylimidazolium Alkylbenzenesulfonate Ionic Liquids, Hiranmayee Kandala Jan 2017

Comprehensive Structural, Thermal And Toxicological Characterization Of 1-Ethyl-3-Methylimidazolium Alkylbenzenesulfonate Ionic Liquids, Hiranmayee Kandala

Electronic Theses and Dissertations

Synthesis and characterization of 1-ethyl-3- methylimidazolium alkylbenzenesulfonate ionic liquids for extraction of lignin from prairie cord grass have been studied. The ionic liquids (ILs) 1-ethyl-3- methylimidazolium benzenesulfonate (EBS), 1-ethyl-3- methylimidazolium toluenesulfonate (ETS) and 1-ethyl-3- methylimidazolium xylenesulfonate (EXS) have been synthesized in this research. An extensive structural, physical, thermal and toxicological characterization has been performed to understand the behavior of these ionic liquids. The reaction yield for the synthesis of EBS, ETS, and EXS ionic liquids was determined to be 91.2 ± 1.5 %, 96.1 ± 0.7 %, 99.0 ± 0.5 % respectively. Spectral analysis using NMR and FTIR confirms the …

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Indexing Walleye Recruitment In Eastern South Dakota Natural Lakes, Todd M. Kaufman Jan 2017

Indexing Walleye Recruitment In Eastern South Dakota Natural Lakes, Todd M. Kaufman

Electronic Theses and Dissertations

Because of the popularity of Walleyes and the need for recurrent supplemental stockings, substantial resources are allocated to their management in eastern South Dakota. For nearly 20 years, fall night electrofishing has been used to monitor age-0 Walleye population parameters, and index future year-class strength in select waters but with limited evaluation. Additionally, knowledge of recruitment patterns and factors that influence those patterns were of interest. Thus, the objectives of this study were: 1) to determine the utility of fall electrofishing age-0 Walleyes to index future year-class strength; 2) to explore whether measures of fall age-0 body size or condition …

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The Relationship Between Recollection, Knowledge Transfer, And Student Attitudes Towards Chemistry, Oluwatobi Omobonike Odeleye Jan 2017

The Relationship Between Recollection, Knowledge Transfer, And Student Attitudes Towards Chemistry, Oluwatobi Omobonike Odeleye

Electronic Theses and Dissertations

Certain foundational concepts, including acid-base theory, chemical bonding and intermolecular forces (IMFs), appear throughout the undergraduate chemistry curriculum. The level of understanding of these foundational concepts influences the ability of students to recognize the relationships between sub-disciplines in chemistry. The purpose of this study was to investigate the relationship between student attitudes towards chemistry and their abilities to recollect and transfer knowledge of IMFs, a foundational concept, to their daily lives as well as to other classes. Data were collected using surveys, interviews and classroom observations, and analyzed using qualitative methods. The data show that while most students were able …

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Intranasal Immunization Of Pigs With Porcine Reproductive And Respiratory Syndrome Virus Like Particles Plus 2’, 3’ –Cgamp Vaccigrade™ Adjuvant Exacerbates Viremia After Virus Challenge, Alexandria Marie Van Noort Jan 2017

Intranasal Immunization Of Pigs With Porcine Reproductive And Respiratory Syndrome Virus Like Particles Plus 2’, 3’ –Cgamp Vaccigrade™ Adjuvant Exacerbates Viremia After Virus Challenge, Alexandria Marie Van Noort

Electronic Theses and Dissertations

Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically devastating virus that causes a persistent infection and spreads quickly. The virus causes abortions in sows and respiratory diseases in young piglets. Current PRRSV vaccines appear to provide limited cross-protection. A virus-like particles (VLPs) vaccine is made up of key PRRSV structural proteins, which could be cross protective among the different strains. We tested PRRSV VLPs as a vaccine candidate with a 2’, 3’-cGAMP VacciGradeTM adjuvant against pigs infected with PRRSV after intranasal immunization to evaluate the immune response and protective efficacy by the VLPs and adjuvant. The VLPs contain …

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Surveillance Of Emerging Livestock Viruses, Faten A. Okda Jan 2017

Surveillance Of Emerging Livestock Viruses, Faten A. Okda

Electronic Theses and Dissertations

Accurate and rapid diagnostic tests are essential for the management and control of emerging infectious diseases. Therefore, the overall goals of studies presented in this dissertation were to provide improved diagnostic and surveillance capabilities for several emerging viral diseases impacting the livestock industry and to provide insight into appropriate control strategies. Recent, severe outbreaks of porcine epidemic diarrhea virus (PEDV) in Asia and North America highlighted the need for well-validated diagnostic tests for identification of PEDV infected animals and evaluation of their immune status. PEDV was first detected in the U.S. in May 2013 and another swine coronavirus, porcine deltacoronavirus …

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Design, Synthesis And Biological Screening Of Novel Cucsinspired Estrone Analogues Towards Treatment Of Hepatocellular Carcinoma, Mater Hussen Mahnashi Jan 2017

Design, Synthesis And Biological Screening Of Novel Cucsinspired Estrone Analogues Towards Treatment Of Hepatocellular Carcinoma, Mater Hussen Mahnashi

Electronic Theses and Dissertations

Cucurbitacins (CUCS) are natural products with highly oxygenated tetracyclic triterpenes produced mostly by Cucurbitaceae family plant. They are known for their therapeutic efficiency with different biological activities, such as anti-inflammatory, hepatoprotective and anti-cancer. Hepatocellular carcinoma (HCC) is the third leading cause of death worldwide. Previous reports have shown the ability of CUCS to inhibit the growth of hepatocellular carcinoma cell lines (HepG-2) significantly. Structural activity relationship studies suggested the potential of the 23, 24 enone side chain of CUCS to bind to the Epidermal Growth Factor Receptor (EGFR). Due to the limited quantities of CUCS upon isolation and the challenges …

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Design, Synthesis And Biological Evaluation Of Novel Estradiol-Triazole Analogs Targeting Epidermal Growth Factor Receptors In Colorectal Cancer, Faez Alotaibi Jan 2017

Design, Synthesis And Biological Evaluation Of Novel Estradiol-Triazole Analogs Targeting Epidermal Growth Factor Receptors In Colorectal Cancer, Faez Alotaibi

Electronic Theses and Dissertations

Colorectal cancer is a life threatening and second cause of death from cancer in the United States. Several proteins (molecular targets) are highly expressed in Colorectal cancer (CRC). Among these molecular targets is Epidermal Growth Factor Receptors (EGFRs) which are overexpressed in cancer cells. Targeting and blocking the downstream signaling could lead to cease cell proliferation and division and hence control the spread of the disease. Molecular modeling is a powerful tool in drug discovery, which has been utilized to design many drug candidates that exhibit biological effect in many disease including cancer. Additionally, pharmacophores such as 1,2,3-triazole showed potency …

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Diaporthe, Soybean Cyst Nematode, And Soybean Aphid: An Evaluation Of Potential Interactions Occurring Among Pests On Soybean In South Dakota, John Philip Posch Jan 2017

Diaporthe, Soybean Cyst Nematode, And Soybean Aphid: An Evaluation Of Potential Interactions Occurring Among Pests On Soybean In South Dakota, John Philip Posch

Electronic Theses and Dissertations

Soybean Glycine max (L.) Merr., is an important crop grown in South Dakota, with an estimated production of $2.33 billion in 2015. However, soybean production in South Dakota is compromised by four pests, the fungal pathogens Diaporthe longicolla (Hobbs) Santos, Vrandecic, and Phillips and Diaporthe caulivora (Athow and Caldwell) Santos, Vrandecic, and Phillips, the soybean cyst nematode Heterodera glycines Ichinohe (SCN), and the soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae). Additionally, these pest can co-occur in fields within South Dakota and the implications associated with interactions among these pests are unknown. We hypothesized that both Diaporthe species would interact with …

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Funding Of Higher Education: Variations In State Funding, Impacts Of State Funding On Differential Tuition, And Variables Impacting Differential Tuition, Michael Holbeck Jan 2017

Funding Of Higher Education: Variations In State Funding, Impacts Of State Funding On Differential Tuition, And Variables Impacting Differential Tuition, Michael Holbeck

Electronic Theses and Dissertations

Public higher education in the United States has seen many changes since the Morrill Act of 1862. Specifically, the funding of higher education has changed greatly over the last half century, from very low tuition and relatively high state subsidies to an increased reliance on tuition to fund higher education. While this funding change has been the national trend, the impact on specific states and universities has varied greatly. This study examines the funding variation between university peers, normalized using state general funds per resident student FTE, to analyze the variation of state funding between states as well as the …

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Studying Microflora Of Semi-Hard Cheese, And Sporulation Within Contact Surface Biofilms, Dalia Khan Jan 2017

Studying Microflora Of Semi-Hard Cheese, And Sporulation Within Contact Surface Biofilms, Dalia Khan

Electronic Theses and Dissertations

The first study was conducted on commercial Italian semi-hard cheese samples that were analyzed microbiologically to understand the effect of fast and slow rate of cooling on cheese microflora during ripening process at different temperatures. A cheese plant noticed an active growth of non-starter lactic acid bacteria, especially the heterofermentative bacteria, in ripened cheese blocks, which weren’t fully cooled. For that, three cheese sample sets, each having 8 samples, were received from the cheese plant for microbiological analysis. The first set included a group of fast cooled cheese samples to 38°F, and the other group was slow cooled to 50°F. …

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Mitigating Risks Of Ochratoxin A Contamination In Oats, Bandana Dhungana Jan 2017

Mitigating Risks Of Ochratoxin A Contamination In Oats, Bandana Dhungana

Electronic Theses and Dissertations

Ochratoxin A (OTA) is a toxic metabolite produced by several species of fungal genera Penicillium and Aspergillus. The major OTA producer in temperate regions (i.e. U.S., Canada, Europe) is Penicillium verrucosum. Oat (Avena sativa), like any other cereal grain, can be contaminated with OTA when storage conditions are favorable for fungal growth. The presence of OTA exceeding the European Union maximum limit of 3 ppb in processed food has been reported in some samples of oat-based breakfast cereals from the US. The use of oat genotypes with limited OTA accumulation would be an effective way to …

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Invertebrate Prey Selectivity Of Channel Catfish (Ictalurus Punctatus) In Western South Dakota Prairie Streams, Erin D. Peterson Jan 2017

Invertebrate Prey Selectivity Of Channel Catfish (Ictalurus Punctatus) In Western South Dakota Prairie Streams, Erin D. Peterson

Electronic Theses and Dissertations

Benthic invertebrates are an important resource for aquatic consumers and should be considered in management of sportfish populations as a factor influencing fishery health. Our study seeks to draw a relationship between invertebrate prey availability and the Channel Catfish Ictalurus punctatus fishery of western South Dakota through diet analysis, but invertebrate surveys in the area have not focused on invertebrates as a prey source. Our objectives were to characterize patterns in availability of the invertebrate prey base in five major river basins, and to evaluate the effects of availability on Channel Catfish prey selectivity and condition. Invertebrates were collected at …

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Intra-Industry Trade In Agricultural Products Between The United States, Nafta And European Union Trading Partners, Shuvo Chakraborty Jan 2017

Intra-Industry Trade In Agricultural Products Between The United States, Nafta And European Union Trading Partners, Shuvo Chakraborty

Electronic Theses and Dissertations

This study provides an overview of trends and identifies country-specific determinants of intra-industry trade (IIT) between the United States (U.S.), the European Union (EU) and the North American Free Trade Agreement (NAFTA) trading partners. We analyze the food and live animal industry at the Standard International Trade Classification (SITC) revision 4 at the 4-digit level to calculate IIT and cover the period between 2007 and 2014. To determine the country-specific determinants of IIT, we used a Generalized Least Squares (GLS) random effect model. Results indicate that the size of an economy, the relative difference in level of economic development, the …

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Perimeter Cropping As A Means Of Reducing Insect Injury And Increasing Crop Yield, Brett L. Owens Jan 2017

Perimeter Cropping As A Means Of Reducing Insect Injury And Increasing Crop Yield, Brett L. Owens

Electronic Theses and Dissertations

The squash vine borer, Melittia cucurbitae Harris, (Lepidoptera: Sesiidae) is a destructive insect known to injure a broad range of cucurbit crops. Many studies have focused on insecticide management of this insect in squash (Cucurbita) and pumpkin (Cucurbita pepo Linnaeus) production; however, little work has been documented on organic approaches to reduce or eliminate injury from this pest to these crops in the Midwest. This study used trap cropping and pollinator mixtures to test whether a reduction of squash vine borer could be achieved in a specific cultivar of pumpkins, Cucurbita pepo ‘Howden’. The study was …

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Reagents And Tools To Identify Epizootic Hemorrhagic Disease Virus-2, Meghan Syrstad Jan 2017

Reagents And Tools To Identify Epizootic Hemorrhagic Disease Virus-2, Meghan Syrstad

Electronic Theses and Dissertations

The purpose of this project was to define monoclonal antibodies against viral proteins from Epizootic Hemorrhagic Disease Virus (EHDV) to be used in quality control of vaccines produced by an industrial partner, and development of tools to identify EHDV. EHDV is an Orbivirus, which also includes Bluetongue Virus and African Horse Sickness, which are all transmitted through arthropod vectors. EHDV causes significant morbidity and mortality in white-tailed deer, but has recently been found to infect cattle. EHDV recently caused significant outbreaks affecting both the farmed and wild cervid industry, however few reagents and tools exist to protect against this disease …

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Improvement Of Milling And Nutritional Quality Characteristics Of Oats Through Genomic Selection, Sudha Neupane Adhikari Jan 2017

Improvement Of Milling And Nutritional Quality Characteristics Of Oats Through Genomic Selection, Sudha Neupane Adhikari

Electronic Theses and Dissertations

Oats can lower cholesterol, reduce risks of type-2 diabetes, and help prevent heart diseases when consumed daily. Therefore, it is important to evaluate and select breeding lines with desirable milling and nutritional quality traits. Genomic selection, which uses genotyping data to predict the breeding value of an individual, is a promising method to increase genetic gain by selecting for quality traits earlier in the line development process. In this study, we collected phenotypic data for three nutritional traits (protein, β- glucan, and fat content) and five milling quality traits (percent plumps, percent thins, percent plump groat, groat percent and thousand …

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Soil Response To Cropping Sequences And Grazing Under Integrated Crop-Livestock System, Hanxiao Feng Jan 2017

Soil Response To Cropping Sequences And Grazing Under Integrated Crop-Livestock System, Hanxiao Feng

Electronic Theses and Dissertations

Integrated crop-livestock system (ICLS) is helpful in diversifying a farm for improving its long-term sustainability and economic benefits. In the United States, the ICLS has been increasing in recent years because of its economic and environmental benefits. However, the impacts of ICLS on soil quality is not well documented in North Dakota. The objective of our study is to assess the impacts of cropping sequences and cattle grazing on the selected soil properties in the crop diversity and livestock integration practice. This study site was established in 2010 at the Dickinson Research Extension Center, Dickinson, North Dakota. The study design …

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Analysis Of Novel Cyanide Antidote Dimethyl Trisulfide For Pharmacokinetic Studies, And Sulfur Mustard Metabolites For Identification Of Biomarker Of Inhaled Dose, Erica Manandhar Jan 2017

Analysis Of Novel Cyanide Antidote Dimethyl Trisulfide For Pharmacokinetic Studies, And Sulfur Mustard Metabolites For Identification Of Biomarker Of Inhaled Dose, Erica Manandhar

Electronic Theses and Dissertations

Cyanide poisoning by accidental or intentional exposure poses a severe health risk. The current FDA approved antidotes for cyanide poisoning can be effective, but each suffers from specific major limitations. Dimethyl trisulfide (DMTS), a sulfur donor that detoxifies cyanide by converting it into thiocyanate, is a promising next generation cyanide antidote. Although a validated analytical method to analyze DMTS is not currently available from any matrix, one will be vital for the approval of DMTS as a therapeutic agent against cyanide poisoning. Hence, a stir bar sorptive extraction (SBSE) gas chromatography – mass spectrometry (GC-MS) method was developed and validated …

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Evaluation Of Woodchip Bioreactors And Phosphorus Adsorption Media For Nutrient Removal From Subsurface Drainage Water, Utsav Thapa Jan 2017

Evaluation Of Woodchip Bioreactors And Phosphorus Adsorption Media For Nutrient Removal From Subsurface Drainage Water, Utsav Thapa

Electronic Theses and Dissertations

Increased subsurface drainage over the past few decades in eastern South Dakota contributed to agricultural water quality problems. Nutrient losses, primarily nitrate-N and dissolved P, from subsurface drainage, have been identified as major contributors to eutrophication in the Great Lakes and Gulf of Mexico. Denitrifying bioreactors and P adsorption structures are edge-of field practices that can be used to protect water quality in waters downstream of subsurface drainage systems. The objectives of this study were to evaluate the effectiveness of woodchip bioreactors and a P adsorption structure in removing nitrate-N and dissolved P from subsurface drainage water. Four woodchip bioreactors …

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Gesi Mainstreaming In Nepal: Representation Of A New Concept Or Re-Presentation Of An Old Concept In A New Package?, Umit Shrestha Jan 2017

Gesi Mainstreaming In Nepal: Representation Of A New Concept Or Re-Presentation Of An Old Concept In A New Package?, Umit Shrestha

Electronic Theses and Dissertations

This study is a process analysis of a newly implemented Gender Equality and Social Inclusion (GESI) mainstreaming process within Ministry of Urban Development in Nepal. The study aims to understand GESI mainstreaming process within theoretical underpinnings of gender mainstreaming and framing. In understanding, the implementation of the GESI mainstreaming process the research also aims to understand the location of the process within different approach towards gender and development. The study analyzes how does GESI Mainstreaming process differs from previous approach in gender and development by understanding whether it creates a new standard for men, women and other vulnerable groups of …

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Effects Of Root Isoflavonoids And Hairy Root Transformation On The Soybean Rhizosphere Bacterial Community Structure, Laura White Jan 2017

Effects Of Root Isoflavonoids And Hairy Root Transformation On The Soybean Rhizosphere Bacterial Community Structure, Laura White

Electronic Theses and Dissertations

Rhizodeposits play a key role in shaping rhizosphere microbial communities. In soybean, isoflavonoids are a key rhizodeposit component that aid in plant defense and enable symbiotic associations with rhizobia. However, it is uncertain if and how they influence rhizosphere microbial communities. Isoflavonoid biosynthesis was silenced via RNA interference of isoflavone synthase in soybean hairy root composite plants. Successive sonication was implemented to isolate soil fractions from 3 different rhizosphere zones at 1 and 3 weeks post planting. PCR amplicons from 16S rRNA gene variable regions V1- V3 and V3-V5 from these soil fractions were analyzed via denaturing gradient gel electrophoresis …

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Local And Systemic Actions Of Gnrh Relating To Estradiol And Associated Reproductive Parameters In Beef Cattle, Jerica J.J. Rich Jan 2017

Local And Systemic Actions Of Gnrh Relating To Estradiol And Associated Reproductive Parameters In Beef Cattle, Jerica J.J. Rich

Electronic Theses and Dissertations

In cattle, estradiol is responsible for modulating many mechanisms involved in successful reproduction. Specifically estradiol is the primary signal for the initiation of standing estrus (Allrich, 1994), and cattle expressing estrus prior to fixed time AI (FTAI) have been reported to have increased preovulatory estradiol concentrations (Perry and Perry, 2008) and increased pregnancy success compared to animals that did not exhibit estrus (Richardson et al., 2016). Gonadotropin releasing hormone, classically, stimulates estradiol production via the two-cell two-gonadotropin hypothesis (Fortune and Quirk, 1988), and GnRH administered systemically in small doses (5 μg) has been reported to elicit an LH pulse similar …

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