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The Promise And Pitfalls Of Genai In Education, Helen Crompton Jan 2024

The Promise And Pitfalls Of Genai In Education, Helen Crompton

STEMPS Faculty Publications

Since ChatGPT was made available to the public, artificial intelligence (AI) has had accelerated growth in the field of education at an unprecedented rate. This study examined the misuses and limitations of ChatGPT and similar generative AI as well as the affordances of these tools for teaching and learning. Using PRISMA protocol, this thematic systematic review examined extant literature from November 2022 to June 2024. The findings reveal five misuses and limitations trends in the literature of cheating, inaccuracies, plagiarism, bias, and data privacy. Affordances highlighted six areas of content creation, personalization, engagement, student coaching and assistance, assessment, and task …


Part 2: Virginia's Metropolitan Areas: Growth, Challenges, And The Road Ahead, Dragas Center For Economic Analysis And Policy, Old Dominion University Jan 2024

Part 2: Virginia's Metropolitan Areas: Growth, Challenges, And The Road Ahead, Dragas Center For Economic Analysis And Policy, Old Dominion University

State of the Commonwealth Reports

In this chapter, we survey the performance of Virginia’s metropolitan areas this decade. We first discuss population trends and the demographic challenges facing metro areas. We then delve into measures of economic activity, including taxable sales and Gross Domestic Product (GDP). We also discuss labor markets in each metro area. We conclude with thoughts about how Virginia can foster growth across its metropolitan areas.


Different Spectrum Of Space Radiation Induced Cognitive Impairments In Radiation-Naïve And Adapted Rats, Richard A. Britten, Arriyam S. Fesshaye, Alyssa Tidmore, Ella N. Tamgue, Paola A. Alvarado-Arriaga Jan 2024

Different Spectrum Of Space Radiation Induced Cognitive Impairments In Radiation-Naïve And Adapted Rats, Richard A. Britten, Arriyam S. Fesshaye, Alyssa Tidmore, Ella N. Tamgue, Paola A. Alvarado-Arriaga

Center for Integrative Neuroscience and Inflammatory Diseases (CINID) Faculty Publications

NASA's decision to resume manned deep space mission, first to the Moon and then Mars, necessitated a detailed assessment of the potential health effects that astronauts may experience on long-duration missions. Multiple studies suggest that there may be significant space radiation (SR)-induced impairment of neurocognitive processes, including advanced executive functions. However, given the multitude of SR-induced changes in the CNS, it is possible that completely different SR-induced sequelae will be induced in previously exposed individuals. Thus, current risk estimates are likely to be pertinent only for the early stages of a deep space mission, and even then only for astronauts …


Utility In Time Description In Priority Best-Worst Discrete Choice Models: An Empirical Evaluation Using Flynn's Data, Sasanka Adikari, Norou Diawara Jan 2024

Utility In Time Description In Priority Best-Worst Discrete Choice Models: An Empirical Evaluation Using Flynn's Data, Sasanka Adikari, Norou Diawara

Mathematics & Statistics Faculty Publications

Discrete choice models (DCMs) are applied in many fields and in the statistical modelling of consumer behavior. This paper focuses on a form of choice experiment, best-worst scaling in discrete choice experiments (DCEs), and the transition probability of a choice of a consumer over time. The analysis was conducted by using simulated data (choice pairs) based on data from Flynn's (2007) 'Quality of Life Experiment'. Most of the traditional approaches assume the choice alternatives are mutually exclusive over time, which is a questionable assumption. We introduced a new copula-based model (CO-CUB) for the transition probability, which can handle the dependent …


The Geometry Of Dynamic Time-Dependent Best-Worst Choice Pairs, Sasanka Adikari, Norou Diawara, Haim Bar Jan 2024

The Geometry Of Dynamic Time-Dependent Best-Worst Choice Pairs, Sasanka Adikari, Norou Diawara, Haim Bar

Mathematics & Statistics Faculty Publications

There has been increasing interest in best–worst discrete choice experiments (BWDCEs) in health economics, transportation research, and other fields over the last few years. BWDCEs have distinct advantages compared to other measurement approaches in discrete choice experiments (DCEs). A systematic study of best–worst (BW) choice pairs can be traced back to the 1990s. Recently, new ideas have been introduced to the subject. Calculating utility helps measure the attractiveness of BW choices. The goal of this paper is twofold. First, we extend the idea of the BW choice pair to include dynamic, time-dependent transition probability and capture utility at each time …


Advancing Environmental Engineering: The Role Of Artificial Intelligence In Sustainable Solutions - A Short Review, Amirreza Talaie, Hesam Kamyab, Ashkan Razmfarsa Jan 2024

Advancing Environmental Engineering: The Role Of Artificial Intelligence In Sustainable Solutions - A Short Review, Amirreza Talaie, Hesam Kamyab, Ashkan Razmfarsa

Management Faculty Publications

Artificial intelligence (AI) has emerged as a transformative force in environmental engineering, offering innovative solutions to complex environmental challenges. From air pollution monitoring and water resource management to waste management, climate change mitigation, and ecological preservation, AI is revolutionizing the way we address environmental issues. Machine learning, neural networks, and other AI technologies are enabling more accurate predictions, optimizing resource use, and improving conservation efforts. However, despite its many advantages, AI also faces challenges such as data availability, energy consumption, ethical concerns, and the need for transparency. This review explores the diverse applications of AI in environmental engineering, highlighting the …


(Re)Producing Orientalism: Industry Logic Of Chinese Mobile Game Re-Skins In The Global App Empire, Yizhou Xu Jan 2024

(Re)Producing Orientalism: Industry Logic Of Chinese Mobile Game Re-Skins In The Global App Empire, Yizhou Xu

Communication & Theatre Arts Faculty Publications

Through the case study of the mobile title Game of Sultans, this article examines the proliferation of iterative and copycat games through the practice of reskins in the mobile game industry. Based on year-long autoethnographic fieldwork working in a Chinese mobile game company, I provide an on-the-ground perspective of how Orientalist representations in reskinned games are instrumentalized through the roles of “cultural brokers” and the work of localization for Western markets. By taking a theoretical and technological understanding of reskins, I argue that reskinned games, as a form of industrial mimicry, while an important aspect of standardized game production, …


Age Of Sensing Empowered Holographic Isac Framework For Nextg Wireless Networks: A Vae And Drl Approach, Apurba Adhikary, Avi Deb Raha, Yu Qiao, Md. Shirajum Munir, Monishanker Halder, Choong Seon Hong Jan 2024

Age Of Sensing Empowered Holographic Isac Framework For Nextg Wireless Networks: A Vae And Drl Approach, Apurba Adhikary, Avi Deb Raha, Yu Qiao, Md. Shirajum Munir, Monishanker Halder, Choong Seon Hong

School of Cybersecurity Faculty Publications

This paper proposes an artificial intelligence (AI) framework that leverages integrated sensing and communication (ISAC), aided by the age of sensing (AoS) to ensure the timely location updates of the users for a holographic MIMO (HMIMO)- enabled wireless network. The AI-driven framework guarantees optimal power allocation for efficient beamforming by activating the minimal number of grids from the HMIMO base station. An optimization problem is formulated to maximize the sensing utility function, aiming to maximize the signal-to-interference-plus-noise ratio (SINR) of the received signal, beam-pattern gains to improve the sensing SINR of reflected echo signals and maximizing the evidence lower bound …


Reverse-Engineering Of Disinformation Campaigns During The War In Ukraine, Lora Pitman, Ava Baratz, Kelly Morgan, Marcy Alvarado Jan 2024

Reverse-Engineering Of Disinformation Campaigns During The War In Ukraine, Lora Pitman, Ava Baratz, Kelly Morgan, Marcy Alvarado

School of Cybersecurity Faculty Publications

Information operations have long been a part of warfare. Disinformation campaigns, in particular, are usually launched by states in order to mislead and confuse populations in adversarial countries, but also to obtain support for their actions from domestic audiences. These campaigns threaten human security, at the individual level, but also state- and even international security. The invasion of Ukraine by Russia came with a new wave of disinformation not only in Ukraine itself, but also in countries from various other continents. This paper studies the characteristics of the spread of disinformation from the first day of the war in February …


Image-To-Mesh Conversion Method For Multi-Tissue Medical Image Computing Simulations, Fotis Drakopoulos, Yixun Liu, Kevin Garner, Nikos Chrisochoides Jan 2024

Image-To-Mesh Conversion Method For Multi-Tissue Medical Image Computing Simulations, Fotis Drakopoulos, Yixun Liu, Kevin Garner, Nikos Chrisochoides

Computer Science Faculty Publications

Converting a three-dimensional medical image into a 3D mesh that satisfies both the quality and fidelity constraints of predictive simulations and image-guided surgical procedures remains a critical problem. Presented is an image-to-mesh conversion method called CBC3D. It first discretizes a segmented image by generating an adaptive Body-Centered Cubic mesh of high-quality elements. Next, the tetrahedral mesh is converted into a mixed element mesh of tetrahedra, pentahedra, and hexahedra to decrease element count while maintaining quality. Finally, the mesh surfaces are deformed to their corresponding physical image boundaries, improving the mesh’s fidelity. The deformation scheme builds upon the ITK open-source library …


Flexible Fitting Of Alphafold2-Predicted Models To Cryo-Em Density Maps Using Elastic Network Models: A Methodological Affirmation, Maytha Alshammari, Jing He, Willy Wriggers Jan 2024

Flexible Fitting Of Alphafold2-Predicted Models To Cryo-Em Density Maps Using Elastic Network Models: A Methodological Affirmation, Maytha Alshammari, Jing He, Willy Wriggers

Computer Science Faculty Publications

Motivation: This study investigates the flexible refinement of AlphaFold2 models against corresponding cryo-electron microscopy (cryo-EM) maps using normal modes derived from elastic network models (ENMs) as basis functions for displacement. AlphaFold2 generally predicts highly accurate structures, but 18 of the 137 models of isolated chains exhibit a TM-score below 0.80. We achieved a significant improvement in four of these deviating structures and used them to systematically optimize the parameters of the ENM motion model.

Results: We successfully refined four AlphaFold2 models with notable discrepancies: lipid-preserved respiratory supercomplex (TM-score increased from 0.52 to 0.69), flagellar L-ring protein (TM-score increased from 0.53 …


Autonomous Strike Uavs In Support Of Homeland Security Missions: Challenges And Preliminary Solutions, Meshari Aljohani, Ravi Mukkamala, Stephan Olariu Jan 2024

Autonomous Strike Uavs In Support Of Homeland Security Missions: Challenges And Preliminary Solutions, Meshari Aljohani, Ravi Mukkamala, Stephan Olariu

Computer Science Faculty Publications

Unmanned Aerial Vehicles (UAVs) are becoming crucial tools in modern homeland security applications, primarily because of their cost-effectiveness, risk reduction, and ability to perform a wider range of activities. This study focuses on the use of autonomous UAVs to conduct, as part of homeland security applications, strike missions against high-value terrorist targets. Owing to developments in ledger technology, smart contracts, and machine learning, activities formerly carried out by professionals or remotely flown UAVs are now feasible. Our study provides the first in-depth analysis of the challenges and preliminary solutions for the successful implementation of an autonomous UAV mission. Specifically, we …


Characterizing Climatic Socio-Environmental Tipping Points In Coastal Communities: A Conceptual Framework For Research And Practice, Julie Elizabeth Shortridge, Anamaria Bukvic, Molly Mitchell, Jesse Goldstein, Tom Allen Jan 2024

Characterizing Climatic Socio-Environmental Tipping Points In Coastal Communities: A Conceptual Framework For Research And Practice, Julie Elizabeth Shortridge, Anamaria Bukvic, Molly Mitchell, Jesse Goldstein, Tom Allen

Political Science & Geography Faculty Publications

The concept of climate tipping points in socio-environmental systems is increasingly being used to describe nonlinear climate change impacts and encourage social transformations in response to climate change. However, the processes that lead to these tipping points and their impacts are highly complex and deeply uncertain. This is due to numerous interacting environmental and societal system components, constant system evolution, and uncertainty in the relationships between events and their consequences. In the face of this complexity and uncertainty, this research presents a conceptual framework that describes systemic processes that could lead to tipping points socio-environmental systems, with a focus on …


To What Extent Are The United States And Nigeria Able To Balance Economic Growth Against Emission Reduction Goals, Bolu Ayankojo Jan 2024

To What Extent Are The United States And Nigeria Able To Balance Economic Growth Against Emission Reduction Goals, Bolu Ayankojo

Political Science & Geography Faculty Publications

The paper examines the link between economic growth and greenhouse gas emissions reduction, focusing on the United States and Nigeria. The study addresses the Nationally Determined Contribution (NDC) targets that ensure average surface temperatures do not exceed 1.5°C above preindustrial levels and are intended to achieve net-zero GHG emissions by 2050. It examines emission reduction strategies in five common sectors: electricity, transportation, industry, cooking, and building. The United States is ahead of Nigeria in achieving its emissions-reduction goals, but challenges persist in some sectors. The study suggests that economic prosperity can be achieved without relying solely on fossil fuels, but …


Recent Progress In Microrna Detection Using Integrated Electric Fields And Optical Detection Methods, Logeeshan Velmanickam, Dharmakeerthi Nawarathna Jan 2024

Recent Progress In Microrna Detection Using Integrated Electric Fields And Optical Detection Methods, Logeeshan Velmanickam, Dharmakeerthi Nawarathna

Electrical & Computer Engineering Faculty Publications

Low-cost, highly-sensitivity, and minimally invasive tests for the detection and monitoring of life-threatening diseases and disorders can reduce the worldwide disease burden. Despite a number of interdisciplinary research efforts, there are still challenges remaining to be addressed, so clinically significant amounts of relevant biomarkers in body fluids can be detected with low assay cost, high sensitivity, and speed at point-of-care settings. Although the conventional proteomic technologies have shown promise, their ability to detect all levels of disease progression from early to advanced stages is limited to a limited number of diseases. One potential avenue for early diagnosis is microRNA (miRNA). …


Ensemble Learning With Sleep Mode Management To Enhance Anomaly Detection In Iot Environment, Khawlah Harahsheh, Rami Al-Naimat, Malek Alzaqebah, Salam Shreem, Esraa Aldreabi, Chung-Hao Chen Jan 2024

Ensemble Learning With Sleep Mode Management To Enhance Anomaly Detection In Iot Environment, Khawlah Harahsheh, Rami Al-Naimat, Malek Alzaqebah, Salam Shreem, Esraa Aldreabi, Chung-Hao Chen

Electrical & Computer Engineering Faculty Publications

The rapid proliferation of Internet of Things (IoT) devices has underscored the critical need for energy-efficient cybersecurity measures. This presents the dual challenge of maintaining robust security while minimizing power consumption. Thus, this paper proposes enhancing the machine learning performance through Ensemble Techniques with Sleep Mode Management (ELSM) approach for IoT Intrusion Detection Systems (IDS). The main challenge lies in the high-power consumption attributed to continuous monitoring in traditional IDS setups. ELSM addresses this challenge by introducing a sophisticated sleep-awake mechanism, activating the IDS system only during anomaly detection events, effectively minimizing energy expenditure during periods of normal network operation. …


Skipresnet: Crop And Weed Recognition Based On The Improved Resnet, Wenyi Hu, Tian Chen, Chunjie Lan, Shan Liu, Lirong Yin Jan 2024

Skipresnet: Crop And Weed Recognition Based On The Improved Resnet, Wenyi Hu, Tian Chen, Chunjie Lan, Shan Liu, Lirong Yin

Electrical & Computer Engineering Faculty Publications

Weeds have a detrimental effect on crop yield. However, the prevailing chemical weed control methods cause pollution of the ecosystem and land. Therefore, it has become a trend to reduce dependence on herbicides; realize a sustainable, intelligent weed control method; and protect the land. In order to realize intelligent weeding, efficient and accurate crop and weed recognition is necessary. Convolutional neural networks (CNNs) are widely applied for weed and crop recognition due to their high speed and efficiency. In this paper, a multi-path input skip-residual network (SkipResNet) was put forward to upgrade the classification function of weeds and crops. It …


Advanced Nested Coaxial Thin-Film Zno Nanostructures Synthesized By Atomic Layer Deposition For Improved Sensing Performance, Pengtao Lin, Lari S. Zhang, Kai Zhang, Helmut Baumgart Jan 2024

Advanced Nested Coaxial Thin-Film Zno Nanostructures Synthesized By Atomic Layer Deposition For Improved Sensing Performance, Pengtao Lin, Lari S. Zhang, Kai Zhang, Helmut Baumgart

Electrical & Computer Engineering Faculty Publications

We report a new synthesis method for multiple-walled nested thin-film nanostructures by combining hydrothermal growth methods with atomic layer deposition (ALD) thin-film technology and sacrificial films, thereby increasing the surface-to-volume ratio to improve the sensing performance of novel ZnO gas sensors. Single-crystal ZnO nanorods serve as the core of the nanostructure assembly and were synthesized hydrothermally on fine-grained ALD ZnO seed films. Subsequently, the ZnO core nanotubes were coated with alternating sacrificial coaxial 3D wrap-around ALD Al2O3 films and ALD ZnO films. Basically, the center nanorod was coated with an ALD 3D wrap-around Al₂O₃ sacrificial layer to realize a nested …


Designing High-Performance Identity-Based Quantum Signature Protocol With Strong Security, Sunil Prajapat, Pankaj Kumar, Sandeep Kumar, Ashok Kumar Das, Sachin Shetty, M. Shamim Hossain Jan 2024

Designing High-Performance Identity-Based Quantum Signature Protocol With Strong Security, Sunil Prajapat, Pankaj Kumar, Sandeep Kumar, Ashok Kumar Das, Sachin Shetty, M. Shamim Hossain

VMASC Publications

Due to the rapid advancement of quantum computers, there has been a furious race for quantum technologies in academia and industry. Quantum cryptography is an important tool for achieving security services during quantum communication. Designated verifier signature, a variant of quantum cryptography, is very useful in applications like the Internet of Things (IoT) and auctions. An identity-based quantum-designated verifier signature (QDVS) scheme is suggested in this work. Our protocol features security attributes like eavesdropping, non-repudiation, designated verification, and hiding sources attacks. Additionally, it is protected from attacks on forgery, inter-resending, and impersonation. The proposed scheme benefits from the traditional designated …


Embedding Software Engineering In Mixed Methods: Computationally Enhanced Risk Communication, Ann Marie Reinhold, Madison H. Munro, Elizabeth A. Shanahan, Ross J. Gore, Barry C. Ezell, Clemente I. Izurieta Jan 2024

Embedding Software Engineering In Mixed Methods: Computationally Enhanced Risk Communication, Ann Marie Reinhold, Madison H. Munro, Elizabeth A. Shanahan, Ross J. Gore, Barry C. Ezell, Clemente I. Izurieta

VMASC Publications

Mixed methods research ameliorates many convergent research challenges within the contemporary sociotechnical landscape. We suggest the integration of software engineering in mixed methods studies is a critical step to address some of the remaining and persistent challenges. One such research challenge where software engineering is particularly well suited is in hazard preparedness—in particular, the creation of risk communication messages to mitigate or prevent harm. Computationally enhanced risk communication is convergent research that integrates software engineering and social science research for the benefit of protecting humans and infrastructure. To this end, we developed a mixed methods framework for the efficient construction …


Cyclegan-Gradient Penalty For Enhancing Android Adversarial Malware Detection In Gray Box Setting, Fabrice Setephin Atedjio, Jean-Pierre Lienou, Frederica F. Nelson, Sachin S. Shetty, Charles A. Kamhoua Jan 2024

Cyclegan-Gradient Penalty For Enhancing Android Adversarial Malware Detection In Gray Box Setting, Fabrice Setephin Atedjio, Jean-Pierre Lienou, Frederica F. Nelson, Sachin S. Shetty, Charles A. Kamhoua

VMASC Publications

Adversarial attacks pose significant threats to Android malware detection by undermining the effectiveness of machine learning-based systems. The rapid increase in Android apps complicates the management of malicious software that can compromise user defense solutions. Many current Android defense techniques rely on deep learning methods. Malicious users exploit GAN-based attacks to achieve adversarial attack transferability and deceive target models by crafting adversarial examples based on known models. We propose a new model based on a Cycle Generative Adversarial Network (CycleGAN) to detect GAN-based attacks. This model incorporates a gradient penalty to enhance the detection rate of the target model. Our …


A Defensive Strategy Against Android Adversarial Malware Attacks, Fabrice Setephin Atedjio, Jean-Pierre Lienou, Frederica F. Nelson, Sachin S. Shetty, Charles A. Kamhoua Jan 2024

A Defensive Strategy Against Android Adversarial Malware Attacks, Fabrice Setephin Atedjio, Jean-Pierre Lienou, Frederica F. Nelson, Sachin S. Shetty, Charles A. Kamhoua

VMASC Publications

Due to the popularity of Android mobile devices over the past ten years, malicious Android applications have significantly increased. Systems utilizing machine learning techniques have been successfully applied for Android malware detection to counter the constantly changing Android malware threats. However, attackers have developed new strategies to circumvent these systems by using adversarial attacks. An attacker can carefully craft a malicious sample to deceive a classifier. Among the evasion attacks, there is the more potent one, which is based on solid optimization constraints: the Carlini-Wagner attack. Carlini-Wagner is an attack that uses margin loss, which is more efficient than cross-entropy …


Designing A Blockchain-Empowered Telehealth Artifact For Decentralized Identity Management And Trustworthy Communication: Interdisciplinary Approach, Xueping Liang, Nabid Alam, Tahmina Sultana, Eranga Bandara, Sachin Shetty Jan 2024

Designing A Blockchain-Empowered Telehealth Artifact For Decentralized Identity Management And Trustworthy Communication: Interdisciplinary Approach, Xueping Liang, Nabid Alam, Tahmina Sultana, Eranga Bandara, Sachin Shetty

VMASC Publications

Background: Telehealth played a critical role during the COVID-19 pandemic and continues to function as an essential component of health care. Existing platforms cannot ensure privacy and prevent cyberattacks.

Objective: The main objectives of this study are to understand existing cybersecurity issues in identity management and trustworthy communication processes in telehealth platforms and to design a software architecture integrated with blockchain to improve security and trustworthiness with acceptable performance.

Methods: We improved personal information security in existing telehealth platforms by adopting an innovative interdisciplinary approach combining design science, social science, and computer science in the health care domain, with prototype …


Enhancing Cyber Resilience Through Traffic Generation Patterns In Complex Networks: A Study On Cascading Failures, Aymar Le Père Tchimwa Bouom, Jean-Pierre Lienou, Wilson Ejuh Geh, Frederica Nelson, Sachin Shetty, Charles Kamhoua Jan 2024

Enhancing Cyber Resilience Through Traffic Generation Patterns In Complex Networks: A Study On Cascading Failures, Aymar Le Père Tchimwa Bouom, Jean-Pierre Lienou, Wilson Ejuh Geh, Frederica Nelson, Sachin Shetty, Charles Kamhoua

VMASC Publications

Network resilience is the capacity of a network to maintain and restore its fundamental operations during or after a failure. This paper investigates the resilience of communication networks with heterogeneous nodes, with host nodes that generate and receive packets and routers that only forward packets. We focus on how traffic generation patterns, defined as the distribution of data packet creation across hosts, affect network resilience. While previous studies identified optimal host placements that balance traffic loads and enhance network performance, this research explores how traffic generation patterns influence network resilience, particularly during cascading failures, where the failure of one node …


Automated Classification Of Elementary Instructional Activities: Analyzing The Consistency Of Human Annotations, Jonathan K. Foster, Peter Youngs, Rachel Van Aswegen, Samarth Singh, Ginger S. Watson, Scott T. Acton Jan 2024

Automated Classification Of Elementary Instructional Activities: Analyzing The Consistency Of Human Annotations, Jonathan K. Foster, Peter Youngs, Rachel Van Aswegen, Samarth Singh, Ginger S. Watson, Scott T. Acton

VMASC Publications

Despite a tremendous increase in the use of video for conducting research in classrooms as well as preparing and evaluating teachers, there remain notable challenges to using classroom videos at scale, including time and financial costs. Recent advances in artificial intelligence could make the process of analyzing, scoring, and cataloguing videos more efficient. These advances include natural language processing, automated speech recognition, and deep neural networks. To train artificial intelligence to accurately classify activities in classroom videos, humans must first annotate a set of videos in a consistent way. This paper describes our investigation of the degree of inter-annotator reliability …


Listeria Adhesion Protein Orchestrates Caveolae-Mediated Apical Junctional Remodeling Of Epithelial Barrier For Listeria Monocytogenes Translocation, Rishi Drolia, Donald B. Bryant, Shivendra Tenguria, Zuri A. Jules-Culver, Jessie Thind, Breanna Amelunke, Donqi Liu, Nicholas L. F. Gallina, Krishna K. Mishra, Manalee Samaddar, Manoj R. Sawale, Dharmendra K. Mishra, Abigail D. Cox, Arun K. Bhunia Jan 2024

Listeria Adhesion Protein Orchestrates Caveolae-Mediated Apical Junctional Remodeling Of Epithelial Barrier For Listeria Monocytogenes Translocation, Rishi Drolia, Donald B. Bryant, Shivendra Tenguria, Zuri A. Jules-Culver, Jessie Thind, Breanna Amelunke, Donqi Liu, Nicholas L. F. Gallina, Krishna K. Mishra, Manalee Samaddar, Manoj R. Sawale, Dharmendra K. Mishra, Abigail D. Cox, Arun K. Bhunia

Biological Sciences Faculty Publications

The cellular junctional architecture remodeling by Listeria adhesion protein-heat shock protein 60 (LAP-Hsp60) interaction for Listeria monocytogenes (Lm) passage through the epithelial barrier is incompletely understood. Here, using the gerbil model, permissive to internalin (Inl) A/B-mediated pathways like in humans, we demonstrate that Lm crosses the intestinal villi at 48 h post-infection. In contrast, the single isogenic (lap− or ΔinlA) or double (lap−ΔinlA) mutant strains show significant defects. LAP promotes Lm translocation via endocytosis of cell-cell junctional complex in enterocytes that do not display luminal E-cadherin. In comparison, InlA facilitates …


Salmonella Detection In Food Using A Hek-Htlr5 Reported Cell-Based Sensor, Esma Eser, Victoria A. Felton, Rishi Drolia, Arun K. Bhunia Jan 2024

Salmonella Detection In Food Using A Hek-Htlr5 Reported Cell-Based Sensor, Esma Eser, Victoria A. Felton, Rishi Drolia, Arun K. Bhunia

Biological Sciences Faculty Publications

The development of a rapid, sensitive, specific method for detecting foodborne pathogens is paramount for supplying safe food to enhance public health safety. Despite the significant improvement in pathogen detection methods, key issues are still associated with rapid methods, such as distinguishing living cells from dead, the pathogenic potential or health risk of the analyte at the time of consumption, the detection limit, and the sample-to-result. Mammalian cell-based assays analyze pathogens’ interaction with host cells and are responsive only to live pathogens or active toxins. In this study, a human embryonic kidney (HEK293) cell line expressing Toll-Like Receptor 5 (TLR-5) …


Receptor-Targeted Next-Generation Probiotics Ameliorate Mammalian Colitis, Nicholas L. F. Gallina, Vignesh Nathan, Akshay Krishnakumar, Dongqi Liu, Rishi Drolia, Nicole Irrizary Tardi, Yang Fu, Manalee Samadar, Shivendra Tenguria, Alvin Cai, Ruth Eunice Centeno-Martinez, Timothy A. Johnson, Abigail Cox, Lavanya Reddivari, Bruce Applegate, Xingjian Bai, Luping Xu, Deepti Tanjore, Ramesh Vemulapalli, Rahim Rahimi, Arun K. Bhunia Jan 2024

Receptor-Targeted Next-Generation Probiotics Ameliorate Mammalian Colitis, Nicholas L. F. Gallina, Vignesh Nathan, Akshay Krishnakumar, Dongqi Liu, Rishi Drolia, Nicole Irrizary Tardi, Yang Fu, Manalee Samadar, Shivendra Tenguria, Alvin Cai, Ruth Eunice Centeno-Martinez, Timothy A. Johnson, Abigail Cox, Lavanya Reddivari, Bruce Applegate, Xingjian Bai, Luping Xu, Deepti Tanjore, Ramesh Vemulapalli, Rahim Rahimi, Arun K. Bhunia

Biological Sciences Faculty Publications

Introduction: a loss of intestinal barrier function, inflammation, and an elevated expression of epithelial heat shock protein 60 (Hsp60) are features of an inflamed bowel. Probiotics have been used to alleviate colitis-induced pathologies, but offer poor adhesion and adaptation to the diseased gut. We hypothesize that enhancing probiotic adhesion in the inflamed bowel may ameliorate such pathologies. Listeria adhesion protein (LAP; 94-kDa acetaldehyde alcohol dehydrogenase) aids Listeria attachment to the epithelial cells by interacting with the mammalian receptor Hsp60. Bioengineered Lactobacillus casei probiotics (BLPs) expressing LAP showed strong interaction with epithelial Hsp60, a high immunomodulatory response, and sustained epithelial barrier …


Ai And Ml-Based Risk Assessment Of Chemicals: Predicting Carcinogenic Risk From Chemical-Induced Genomic Instability, Ajay Vikram Singh, Preeti Bhardwaj, Peter Laux, Prachi Pradeep, Madleen Busse, Andreas Luch, Akihiko Hirose, Christopher J. Osgood, Michael W. Stacey Jan 2024

Ai And Ml-Based Risk Assessment Of Chemicals: Predicting Carcinogenic Risk From Chemical-Induced Genomic Instability, Ajay Vikram Singh, Preeti Bhardwaj, Peter Laux, Prachi Pradeep, Madleen Busse, Andreas Luch, Akihiko Hirose, Christopher J. Osgood, Michael W. Stacey

Biological Sciences Faculty Publications

Chemical risk assessment plays a pivotal role in safeguarding public health and environmental safety by evaluating the potential hazards and risks associated with chemical exposures. In recent years, the convergence of artificial intelligence (AI), machine learning (ML), and omics technologies has revolutionized the field of chemical risk assessment, offering new insights into toxicity mechanisms, predictive modeling, and risk management strategies. This perspective review explores the synergistic potential of AI/ML and omics in deciphering clastogen-induced genomic instability for carcinogenic risk prediction. We provide an overview of key findings, challenges, and opportunities in integrating AI/ML and omics technologies for chemical risk assessment, …


Abalones At Risk: A Global Red List Assessment Of Haliotis In A Changing Climate, Howard Peters, Gina M. Ralph, Laura Rogers-Bennett Jan 2024

Abalones At Risk: A Global Red List Assessment Of Haliotis In A Changing Climate, Howard Peters, Gina M. Ralph, Laura Rogers-Bennett

Biological Sciences Faculty Publications

There is increasing awareness that marine invertebrates such as abalones are at risk from the combined stressors of fishing and climate change. Abalones are an important marine fishery resource and of cultural importance to Indigenous and non-Indigenous people. A highly priced marine delicacy, they are inherently vulnerable: individuals are slow-growing and long-lived and successful reproduction requires dense assemblages. However, their global conservation status is poorly understood. Using IUCN Red List methodology, we assessed the extinction risk to all 54 species of abalone (genus Haliotis). Of the 21 fished commercially for human consumption either now and/or in the past, 15 …