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Comparative Study Of Selenium Requirements Of Three Phytoplankton Species: Gymnodinium Catenatum, Alexandrium Minutum (Dinophyta) And Chaetoceros Cf. Tenuissimus (Bacillariophyta, Martina A. Doblin, Susan I. Blackburn, Gustaaf M. Hallegraeff

OES Faculty Publications

This study investigated the selenium (Se) requirements of three phytoplankton species which commonly bloom in southern Australian estuaries. The present study showed that the toxic dinoflagellate Gymnodinium catenatum Graham had an obligate requirement for Se (IV) in culture. After two transfers (~ 4 weeks ≈ 7 generations) in Se-deficient seawater medium, this phytoplankton species exhibited a decline in growth rate (25%) and biomass yield (90%), while complete cessation of cell division occurred under prolonged (8 weeks ≈ 12 generations) Se starvation. Addition of 10^-9-10^-7 M H₂SeO₃ to nutrient-enriched seawater medium resulted in increased G.catenatum …

Limitation Of Algal Growth By Iron Deficiency In The Australian Subantarctic Region, Peter N. Sedwick, Giacomo R. Ditullio, David A. Hutchins, Philip W. Boyd, F. Brian Griffiths, A. Clive Crossley, Thomas W. Trull, Bernard Queguiner

OES Faculty Publications

In March 1998 we measured iron in the upper water column and conducted iron- and nutrient-enrichment bottle-incubation experiments in the open-ocean Subantarctic region southwest of Tasmania, Australia. In the Subtropical Convergence Zone (∼42°S, 142°E), silicic acid concentrations were low (< 1.5μM) in the upper water column, whereas pronounced vertical gradients in dissolved iron concentration (0.12-0.84 nM) were observed., presumably reflecting the interleaving of Subtropical and Subantarctic waters, and mineral aerosol input. Results of a bottle-incubation experiment performed at this location indicate that phytoplankton growth rates were limited by iron deficiency within the iron-poor layer of the euphotic zone. In the Subantarctic water mass (∼46.8°S, 142°E), low concentrations of dissolved iron (0.05-0.11nM) and silicic acid (< 1μM) were measured throughout the upper water column, and our experimental results indicate that algal growth was limited by iron deficiency. These observations suggest that availability of dissolved iron is a primary factor limiting phytoplankton growth over much of the Subantarctic Southern Ocean in the late summer and autumn.

Trans-Resveratrol Inhibits Calcium Influx In Thrombin-Stimulated Human Platelets, Yuliya Dobrydneva, Roy L. Williams, Peter F. Blackmore

Chemistry & Biochemistry Faculty Publications

1 The phytoestrogenic compound trans-resveratrol (trans-3,5,4'-trihydroxystilbene) is found in appreciable quantities in grape skins and wine. It has been shown that both products rich in trans-resveratrol and pure trans-resveratrol inhibit platelet aggregation both in vivo and in vitro. However the mechanism of this action still remains unknown.

2 An essential component of the aggregation process in platelets is an increase in intracellular free Ca²⁺ ([Ca²⁺]_i). Ca²⁺ must enter the cell from the external media through specific and tightly regulated Ca²⁺ channels in the plasma membrane. The objective …

An Iron-Based Ecosystem Model Of The Central Equatorial Pacific, Carrie L. Leonard, Charles R. Mcclain, Ragu Murtugudde, Eileen E. Hofmann, Lawrence W. Harding Jr.

CCPO Publications

The central and eastern equatorial Pacific region is characterized by lower than expected phytoplankton biomass and primary production given the relatively high ambient nitrate concentrations. These unusual conditions have spawned several field programs and laboratory experiments to determine why this high nitrate-low chlorophyll pattern persists in this region. To synthesize the results from these field programs, as well as providing additional evidence in support of the iron hypothesis, we developed a one-dimensional, nine-component ecosystem model of 0 degrees N 140 degrees W. The model components include two phytoplankton size fractions, two zooplankton size fractions, two detrital size fractions, dissolved iron, …

Modeling The Msx Parasite In Eastern Oyster (Crassostrea Virginica) Populations. Iii. Regional Application And The Problem Of Transmission, Eric N. Powell, John M. Klinck, Susan E. Ford, Eileen E. Hofmann, Stephen J. Jordon

CCPO Publications

A model of transmission for Haplosporidium nelsoni, the disease agent for MSX disease, is developed and applied to sites in Delaware Bay and Chesapeake Bay. The environmental factors that force the oyster population- H. nelsoni model are salinity, temperature, food, and total suspended solids. The simulated development of MSX disease was verified using 3 time series of disease prevalence and intensity: 1960 to 1970 and 1980 to 1990 for Delaware Bay, and 1980 to 1994 for Chesapeake Bay, and for a series of sites covering the salinity gradient in each bay. Additional simulations consider the implications of assumptions made …

Modeling The Msx Parasite In Eastern Oyster (Crassostrea Virginica) Populations. Ii. Salinity Effects, Michelle C. Paraso, Susan E. Ford, Eric N. Powell, Eileen E. Hofmann, John M. Klinck

CCPO Publications

An oyster population model coupled with a model for Haplosporidium nelsoni, the causative agent of the oyster disease MSX, was used with salinity time-series constructed from Delaware River flow measurements to study environmentally-induced variations in the annual cycle of this disease in Delaware Bay oyster populations. Model simulations for the lower Bay (high salinity) sire reproduced the annual cycle observed in lower Delaware Bay. Simulations at both upper Bay (low salinity) and lower Bay sites produced prevalences and intensities that were consistent with field observations. At all sites, low freshwater discharge resulted in increased disease levels, whereas high freshwater …

Modeling The Msx Parasite In Eastern Oyster (Crassostrea Virginica) Populations. I. Model Development, Implementation, And Verification, Susan Ford, Eric Powell, John Klinck, Eileen Hofmann

CCPO Publications

A mathematical model simulating the host-parasite-environmental interactions of eastern oysters (Crassostrea virginica) and the pathogen, Haplosporidium nelsoni, which causes MSX disease, has been developed. The model has 2 components. One replicates the infection process within the oyster and the other simulates transmission. The infection-development component relies on basic physiological processes of both host and parasite, modified by the environment, to reproduce the observed annual prevalence cycle of H. nelsoni. Equations describing these rates were constructed using data from long-term field observations, and field and laboratory experiments. In the model, salinity and temperature have direct effects upon …

Frontogenesis In The North Pacific Oceanic Frontal Zones--A Numerical Simulation, Michael S. Dinniman, Michele M. Rienecker

CCPO Publications

A primitive equation model [Geophysical Fluid Dynamics Laboratory's (GFDL's) MOM 2] with one degree horizontal resolution is used to simulate the seasonal cycle of frontogenesis in the subarctic frontal zone (SAFZ) and the subtropical frontal zone (STFZ) of the North Pacific Ocean. The SAFZ in the model contains deep (greater than 500 m in some places) regions with seasonally varying high gradients in temperature and salinity. The gradients generally weaken toward the east. The STFZ consists of a relatively shallow (less than 200 m in most places) region of high gradient in temperature that disappears in the summer/fall. The high …

Modification Of No, Po, And No/Po During Flow Across The Bering And Chukchi Shelves: Implications For Use As Arctic Water Mass Tracers, Lee W. Cooper, Glenn F. Cota, Lawrence R. Pomeroy, Jacqueline M. Grebmeier, Terry E. Whitledge

CCPO Publications

The NO and PO tracers (9[NO₃-] + 02 and 135[PO₄-] + 02, respectively,) and their derivative NO/PO have found increasing use in Arctic water mass analyses for identifying the specific basin or shelf areas from which surface waters originate, based upon assumed differences in Pacific- and Atlantic-derived content and basin-to-basin differences within the Arctic. Following shipboard sampling in June-September 1993 and May-June 1994, both north and south of Bering Strait, we have found evidence that Pacific-derived waters flowing north to Bering Strait do not necessarily have any unique NO, PO, or NO/PO identity that would permit …

Field Sampling And Necropsy Examination Of Fish, A. S. Kane, A. Baya, R. Reimschuessel, K. M. St. Pé, C. A. Poukish, C. P. Driscoll

Virginia Journal of Science

This paper presents an overview of observational and fish sampling tech­niques for investigating fish lesions, morbidity and mortality. These sam­pling techniques and investigations are much like detective work and require attention to detail, common sense, technical proficiency and experience. To solve the mystery of a fish kill, the investigator must use available evidence and clues to piece together a series of events that often have long since passed. The cause of these field events may be chemical, biological or physical; more often, it is some combination of these. An initial categorization approach may be used to reduce the great number …

Pcr And Fish Detection Extends The Range Of Pfiesteria Piscicida In Estuarine Waters, P. A. Rublee, J. Kempton, E. Schaefer, J.M. Burkholder, H. B. Glasgow Jr., David Oldach

Virginia Journal of Science

PCR and fluorescent in situ hybridization probes were used to assay for the presence of the dinoflagellate Pfiesteria piscicida in 170 estuarine water samples collected from New York to northern Florida. 20% of samples tested positive for the presence of P. piscicida, including sites where fish kills due to Pfiesteria have occurred and sites where there was no historical evidence of such events. The results extend the known range of P. piscicida northward to Long Island, New York. The results also suggest that P. piscicida is common, and normally benign, inhabitatant of estuarine waters of the eastern US.

Virginia's Pfiesteria Monitoring Program: Water Quality, Roger K. Everton, Wick T. Harlan, Jonathan W. Priest, Mark S. Alling

Virginia Journal of Science

During the summer and early fall of 1998, 34 estuarine stations in Virginia were sampled for Pfiesteria like organisms (PLOs) and associated water quality conditions. Stations were sampled either bimonthly (20 stations) or monthly (14 stations) from June to October 1998. At each station, a set of live and Lugol' s preserved samples were collected for presumptive counts of PLOs and water quality conditions were determined. Water quality parame­ters measured included standard field parameters, nutrients (total, dissolved, and particulate), chlorophyll a, and conventional water quality indicators. Although PLOs were relatively low in 1998 as compared to 1997, presump­tive PLO counts …

A Cohort Study To Determine The Epidemiology Of Estuary-Associated Syndrome, Elizabeth Turf, Lily Ingsrisawang, Megan Turf, J.D. Ball, Michael Stutts, John Taylor, Suzanne Jenkins

Virginia Journal of Science

From the Introduction:

Estuary-Associated Syndrome (EAS) is the name given to a potential illness characterized primarily by changes in an individual's cognitive abilities, including acute onset of memory loss or the sudden inability to solve simple problems. Other possible signs of illness include respiratory symptoms, skin rash, or gastrointestinal distress. This illness appears to arise following exposure to toxin produced by Pfiesteria piscicida, or other toxic dinoflagellates, that resides in estuary waters.

….

In order to learn more about this possible syndrome and to determine if a causal relationship exists between association to waters containing Pfiesteria or other toxic …

Monitoring Results For Pfiesteria Piscidida And Pfiesteria-Like Organisms From Virginia Waters In 1998, Harold G. Marshall, David W. Seaborn, Jennifer Wolny

Virginia Journal of Science

Results of an extensive 1998 monitoring program for the presence of Pfiesteria-like organisms (PLO) in Virginia estuaries indicate these dinoflagellates are widely distributed in both the water column, and as cysts in the sediment, however Pfiesteria piscicida was not detected at this time. The highest concentrations of PLO were in estuaries along the Virginia shore line of the Potomac River, and in western Chesapeake Bay estuaries from the Little Wicomico River to the Rappahannock River. The most common PLO included Cryptoperidiniopsis sp. and Gymnodinium galatheanum. The lowest PLO concentrations were at ocean side locations. PLO were also …

Growth And Feeding Studies On The Algal Feeding Stage Of A Pfiesteria-Like Dinoflagellate, David W. Seaborn, A. Michelle Seaborn, William M. Dunstan, Harold G. Marshall

Virginia Journal of Science

The dinoflagellate Cryptoperidiniopsis sp. was isolated from sediment samples taken from Virginia estuaries, and established in culture for subsequent growth and feeding studies. The maximum abundance, or yield, of Cryptoperidiniopsis is exponentially related to the concentration of algal prey and is saturated at about 4.00 X 10⁵ mL^-1. Salinity from 10-20 ppt and temperature between 15-25 C have no effect on the yield of this form of Cryptoperidiniopsis. Light intensity has a secondary effect in that the algal prey reproduces more quickly in higher light as they are being grazed. Growth rates of Cryptoperidiniopsis were …

Water Quality Relationships To Concentrations Of Pfiesteria-Like Organisms In Virginia Estuaries For 1998, Everett P. Weber, Harold G. Marshall

Virginia Journal of Science

A series of statistical analyses were performed to identify the relationship between abundance of dinoflagellates grouped as Pfiesteria-like organisms and a set of 25 water quality variables from May through October of 1998 at 41 estuarine locations. Although regions were identified in relation to seasonal density of cells present, there were no strong relationships to specific water quality variables. Factors that may have influenced these results included: a) several species were included in the group analyzed and this composite did not respond as a unit to changing environmental conditions; b) cell concentrations were low and there were a large …

Continental Runoff And Effects On The North Atlantic Ocean Subtropical Mode Water, Larry P. Atkinson, Chester E. Grosch

CCPO Publications

Interannual salinity variations in North Atlantic Subtropical Mode Water (STMW) are well known although the cause is less well understood. Attempts to model local salinity variation with local evaporation and precipitation have not been successful and some authors invoke advection of low salinity water as the cause. Examination of the STMW and North American river runoff data suggests that runoff may partly explain the salinity variations. It is known that low salinity water resulting from Mississippi River outflow is transported well past Cape Hatteras. Spearman Rank Correlation analysis and spectra and cross-spectra Fourier correlation analysis both show that river flow …

Modeling The Effects Of Doliolids On The Plankton Community Structure Of The Southeastern Us Continental Shelf, A. G. Edward Haskell, Eileen E. Hofmann, Gustav-Adolf Paffenhofer, Peter G. Verity

CCPO Publications

A model of the lower trophic levels that consists of a system of coupled ordinary differential equations was developed to investigate the time-dependent behavior of doliolid populations associated with upwelling features on the outer southeastern US continental shelf. Model equations describe the interactions of doliolids with two phytoplankton size fractions, five copepod developmental stages and a detrital pool. Additional equations describe nitrate and ammonia. Model dynamics are based primarily upon data obtained from field and laboratory experiments for southeastern US continental shelf plankton populations. Variations on a reference simulation, which represents average upwelling conditions without doliolids, were carried out to …

Foreward, Virginia Journal Of Science

Virginia Journal of Science

Foreward to the Winter 1999 issue of Virginia Journal of Science, discussing Pfiesteria Piscicida, written by Harold G. Marshall.

Interest Of Department Of Correctional Education Personnel In Attending Old Dominion University Via The Teletechnet Distance Education System, Dennis D. Traverse

OTS Master's Level Projects & Papers

This study will determine: 1. If Department of Correctional Education full-time academic teachers are interested in taking the courses required to complete the Master of Science in Education degree with a concentration in correctional education programs via the Old Dominion University TELETECHNET Distance Education Program; 2. What content should be included in the concentration courses for correctional education personnel.

A Study To Categorize Adult Education Literature Through Donlevy And Donlevy's Template Of Perspectives, Linda A. Mason

OTS Master's Level Projects & Papers

The following objectives were established to address this problem: 1. How effectively can adult education literature be categorized by using the Donlevy and Donlevy Template of Perspectives? 2. What recommendations can be made from these perspectives for the teaching of adult education principles?

Methods Of Teaching Acting, Eric Allen Frenck

OTS Master's Level Projects & Papers

The objective of this paper is to find the critical attribute required to teach acting to beginner-level students, whether it is a specific method of teaching, innovative acting drills used, or the type of classroom environment one must foster. The latter is strongly suspected, as the first two options above are somewhat empirical and possibly, if not readily, available and therefore easily taught to anyone desiring to learn to teach acting.

An Appraisal Of The Accelerated Medical Laboratory Technician Program At Thomas Nelson Community College, Christine T. Gillerlain

OTS Master's Level Projects & Papers

The goals of this study were to answer the following questions: 1. Does the accelerated MLT program provide the same educational experiences as the traditional MLT program? 2. Does a change to a more concentrated course schedule change the Navy MLT student's opinions about the program in which they are enrolled? 3. What are the major weaknesses and the major benefits of the accelerated program as indicated by the student? 4. How does the student performance compare in the accelerated MLT program with the traditional MLT program as measured by the final grades in Hematology, Blood Bank, Urinalysis, and Immunology?

Aneuploidy Frequencies In Semen Fractions From Ten Oligoasthenoteratozoospermic Patients Donating Sperm For Intracytoplasmic Sperm Injection, Jerome Pfeffer, Myung-Geol Pang, Stanton F. Hoegerman, Christopher J. Osgood, M. W. Stacey, Jacob Mayer, Sergio Oehninger, William G. Kearns

Biological Sciences Faculty Publications

Objective: To determine aneuploidy frequencies in pellet and swim-up semen fractions from 10 infertile men with severe oligoasthenoteratozoospermia (OAT) who were donating sperm for intracytoplasmic sperm injection and to determine whether the swim-up isolation method would successfully separate aneuploid from haploid sperm.

Design: Prospective study.

Setting: Infertility clinic and molecular genetics laboratory.

Patient(s): Ten patients with severe OAT.

Intervention(s): Cytogenetic analyses by fluorescence in situ hybridization to determine aneuploidy frequencies for chromosomes 1, 13, 18, 21, X, and Y in sperm from swim-up and pellet fractions.

Main Outcome Measure(s): Gametic aneuploidy was scored in sperm fractions separated by the swim-up …

Anomie, Deviant Behavior, And The Olympics, Brian K. Payne, Bruce L. Berg

Sociology & Criminal Justice Faculty Publications

Criminal and deviant behavior is known to occur in all places, settings, and times. The Olympics, both Summer and Winter, are not immune to deviant behavior. This paper focuses on the specific types of deviant and criminal acts arising out of the Olympic settings and the anomic factors that possibly lead to deviance in this particular arena. The way that athletes are conformists, innovators, ritualists, and retreatists is considered along with the way that norm confusion influences the Olympic event. Also considered is the role of norm saturation in confusing the way actors interpret appropriate behavior in this setting. Implications …

Monitoring Results For Pfiesteria Piscidida And Pfiesteria-Like Organisms From Virginia Waters In 1998, Harold G. Marshall, David W. Seaborn, Jennifer Wolny

Biological Sciences Faculty Publications

Results of an extensive 1998 monitoring program for the presence of Pfiesteria-like organisms (PLO) in Virginia estuaries indicate these dinoflagellates are widely distributed in both the water column, and as cysts in the sediment, however Pfiesteria piscicida was not detected at this time. The highest concentrations of PLO were in estuaries along the Virginia shore line of the Potomac River, and in western Chesapeake Bay estuaries from the Little Wicomico River to the Rappahannock River. The most common PLO included Cryptoperidiniopsis sp. and Gymnodinium galatheanum. The lowest PLO concentrations were at ocean side locations. PLO were also …

The Cause And Consequence Of Ontogenetic Changes In Social Aggregation In New Zealand Spiny Lobsters, Mark J. Butler Iv, Alistair B. Macdiarmid, John D. Booth

Biological Sciences Faculty Publications

Ontogenetic changes in the behavior, spatial distribution, or habitat use of a species are presumably adaptations to ecological forces that differ in their effect on various life stages. The New Zealand rock lobster Jasus edwardsii is one of several species of spiny lobster that exhibits dramatic ontogenetic shifts in sociality and spatial distribution, and we tested whether such changes are adaptive. We first surveyed several natural populations of J. edwardsii to document size-specific differences in aggregation. To determine if chemical cues discharged by conspecifics promote aggregation of certain ontogenetic stages, we tested the responsiveness of lobsters of 3 ontogenetic stages …

Growth And Feeding Studies On The Algal Feeding Stage Of A Pfiesteria-Like Dinoflagellate, David W. Seaborn, A. Michelle Seaborn, William M. Dunstan, Harold G. Marshall

Biological Sciences Faculty Publications

The dinoflagellate Cryptoperidiniopsis sp. was isolated from sediment samples taken from Virginia estuaries, and established in culture for subsequent growth and feeding studies. The maximum abundance, or yield, of Cryptoperidiniopsis is exponentially related to the concentration of algal prey and is saturated at about 4.00 X 10⁵ mL^-1. Salinity from 10-20 ppt and temperature between 15-25 C have no effect on the yield of this form of Cryptoperidiniopsis. Light intensity has a secondary effect in that the algal prey reproduces more quickly in higher light as they are being grazed. Growth rates of Cryptoperidiniopsis were …

Brain Sex: How The Media Report And Distort Brain Research, Janet M. Bing

English Faculty Publications

Testosterone gives men a particular advantage in that it is focusing and galvanizing a brain that is already, by its very structure, more focused than the female. Remember that the male brain is a tidier affair, each function in its special place . . . Biology, then, every bit as much as social conditioning, militates against a strongly feminine role in areas traditionally regarded as male preserves.

Nucleotide Sequence Of The Streptococcin A-Ff22 Lantibiotic Regulon: Model For Production Of The Lantibiotic Sa-Ff22 By Strains Of Streptococcus Pyogenes, Robert E. Mclaughlin, Joseph J. Ferretti, Wayne L. Hynes

Biological Sciences Faculty Publications

Streptococcin A-FF22 (SA-FF22) is a type AII linear lantibiotic produced by Streptococcus pyogenes strain FF22. Sequence analysis of an approximate 10 kb region of DNA showed it to contain nine open reading frames arranged in three operons responsible for regulation, biosynthesis and immunity of SA-FF22. This region is organized similarly to the Lactococcus lactis lacticin 481 region, however, unlike lacticin 481, a two-component regulatory system is essential for SA-FF22 production. located immediately downstream of the scn region is a putative transposase gene, the presence of which supports earlier data that indicated a mobile nature to this region. (©) 1999 Published …