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K+-Induced Smooth Muscle Calcium Sensitization Requires Rhoa Kinase (Rok) Translocation To Caveolae Which Is Inhibited In Non-Neuronal Cell Memory, Nicole Hairrell Urban

Theses and Dissertations in Biomedical Sciences

KC1 causes smooth muscle contraction by elevating intracellular free calcium ([Ca²⁺]i₎, while receptor stimulation activates an additional mechanism termed Ca²⁺- sensitization that can involve activation of ROK and PKC. However, recent studies support the hypothesis that KC1 may also increase Ca²⁺-sensitivity (36). Our data showed that the PKC inhibitor, GF-109203X, did not, while the ROK inhibitor, Y-27632, did inhibit KCl-induced tonic (5’) force and myosin light chain (MLC) phosphorylation in rabbit artery. Y-27632 also inhibited Bay K-8644- and ionomycin-induced MLC phosphorylation and force, but did not inhibit KCl-induced calcium entry or peak …

Discourse Of Evil: Speaking Terrorism To Silence, Galip Isen

Reconstruction: Studies in Contemporary Culture

[First paragraph]

That the impact of terrorism is equal to the reaction it elicits may be a truism, but one that deserves further probing as demonstrated by the way that reaction is put to use politically. Whichever of 150-strong definitions [1] is accepted, the consequential aspect of terrorism is ideational: the assault targets the concept and experience of normal in the lives and minds of ordinary people. Several writers argue that terrorism is generally inefficacious, strategically counterproductive and mostly unsuccessful as a method of attaining socio-political objectives [2]. However, overall public response to 11 September 2001 has been so profound …

Homeland (In)Security : Roots And Displacement, From New York, To Toronto, To Salt Lake City, Marusya Bociurkiw

Reconstruction: Studies in Contemporary Culture

[First paragraph]

On September 11, 2001, broadcast and print media around the world narrated the destruction of New York City's World Trade Center and the deaths of thousands of its occupants. This paper will examine the ways in which television and its appendages (the telephone, the internet, the newspaper) operated to organize the discursive meanings of this traumatic event. I will propose that between September 11 and February 2002's Winter Olympics in Salt Lake City, Utah, ideas about home, roots and rootedness (and their Others: foreignness, homelessness, nomadicism) operated as a discursive mapping of that which could otherwise not be …

The End Of Science Fiction: When Technological Extrapolation Hits A Wall Across The Future, James John Bell

Reconstruction: Studies in Contemporary Culture

[First paragraph]

Science fiction, as a genre of popular fiction, can trace its roots as far back as the 2nd century AD, where fantastical worlds were conjured up in order to comment on current beliefs. The Ultimate Encyclopedia of Science Fiction (1996) outlines a very specific ancestry:

[Science Fiction] is a descendant of the type of prose fiction sometimes referred to as Lucianic Satire (after Lucian of Samosata, a Greek writer of the 2nd century AD). Lucianic Satire -- also commonly known as Menippean Satire after an earlier writer, Menippus, whose works are now lost -- is a kind of …

On David Koplow's Smallpox: The Fight To Eradicate A Global Scourge, Lisa Donovan, David Alan Sapp

Reconstruction: Studies in Contemporary Culture

[First paragraph]

The world is free from the variola virus, more commonly known to us as the virus responsible for smallpox -- or at least it is almost free from it. Since the World Health Organization's (WHO) successful eradication campaign ended officially on December 9, 1979, smallpox no longer exists in its natural state, yet it does remain in securely isolated freezers in two locations: the Centers for Disease Control and Prevention facility in Atlanta, Georgia, and the Russian State Research Center of Virology and Biotechnology near Novosibirsk. In Smallpox: The Fight to Eradicate a Global Scourge, David Koplow …

Mechanical Properties Of The Integument Of The Common Gartersnake, Thamnophis Sirtalis (Serpentes: Colubridae), Gabriel Rivera

Biological Sciences Theses & Dissertations

The evolution of the ophidian feeding mechanism has involved substantial morphological restructuring associated with the ability to ingest relatively large prey. Previous studies examining the morphological consequences of macrophagy have concentrated on modifications of the skull and cephalic musculature. Although it is evident that macrophagy requires highly compliant skin, the mechanical properties of the ophidian integument have received limited attention, particularly in the context of feeding. I examined mechanical properties of skin along the body axis in male Thamnophis sirtalis (Colubridae). From each of 11 specimens, I examined nine circumferential strips of skin, each spanning 10 ventral scales, spaced at …

Geographic Variation In The Morphology Of Crotalus Horridus (Serpentes: Viperidae), John Robert Allsteadt

Biological Sciences Theses & Dissertations

The Timber Rattlesnake (Crotalus horridus) occurs in discontinuous populations throughout the eastern and central United States. The species exhibits high levels of polymorphism in morphological traits, especially in coloration and pattern. Previous studies recognized either distinct northern and southern subspecies or three regional morphs (northern, southern, and western), but conflicting data sets and limited geographic sampling of previous studies have left the relationships among those regional variants unclear. In this study, univariate and multivariate statistics, together with a geographic information system, were used to analyze geographic variation in 36 morphological characters recorded from 2,420 specimens of C. horridus …

Competency-Based Education: Implications For Curricular Development Of The Allied Dental Science Program At The Jordan University Of Science And Technology, Suhair Ref' At Obeidat

Dental Hygiene Master's Papers and Projects

Preface:

The content of this document represents a review of the best practices and principles in competency-based dental and allied dental education. This information is offered in the spirit of collegiality with the intent of improving the allied dental education in general and the dental hygiene education in particular in Jordan. Recommendations presented should serve as a basis for discussion and decision-making by all involved in the Allied Dental Science Program education and administration at the Jordan University of Science and Technology, including the Dean of the Applied Medical Sciences Department and the Allied Dental Science Program Director and Faculty.

Minutes Of The Council Meeting- November 2002, Virginia Journal Of Science

Virginia Journal of Science

Full proceedings of the Council Meeting of the Virginia Academy of Science at the Science Museum of Virginia, November 2, 2002.

Minutes Of The Virginia Academy Of Science Council Meeting- May 2002, Virginia Journal Of Science

Virginia Journal of Science

Full proceedings of the Council Meeting of the Virginia Academy of Science at Hampton University on May 24, 2002

An Unusually Colored Red-Backed Salamander (Plethodon Cinerus) From Northern Virginia, Joseph C. Mitchell, Thomas K. Pauley, C. Todd Georgel

Virginia Journal of Science

We describe an orange-pink, patternless, translucent morph of the Red-backed Salamander (Plethodon cinereus) found in northern Virginia on 27 September 2001. This is the first description of this unusual phenotype in this terrestrial salamander.

Necrology: Patrick F. Scanlon, Virginia Journal Of Science

Virginia Journal of Science

Dr. Patrick F. Scanlon, Professor of fisheries and wildlife sciences, died on Wednesday, March 4, 2003.

The Effects Of Intense Submicrosecond Electrical Pulses On Cells, Jingdong Deng, Karl H. Schoenbach, E. Stephen Buescher, Pamela S. Hair, Paula M. Fox, Stephen J. Beebe

Bioelectrics Publications

A simple electrical model for living cells predicts an increasing probability for electric field interactions with intracellular substructures of both prokaryotic and eukaryotic cells when the electric pulse duration is reduced into the submicrosecond range. The validity of this hypothesis was verified experimentally by applying electrical pulses (durations 100 μs– 60 ns, electric field intensities 3–150 kV/cm) to Jurkat cells suspended in physiologic buffer containing propidium iodide. Effects on Jurkat cells were assessed by means of temporally resolved fluorescence and light microscopy. For the longest applied pulses, immediate uptake of propidium iodide occurred consistent with electroporation as the cause of …

Hybridization Between The Watersnakes Nerodia Sipedon And Nerodia Fasciata, In The Carolinas: A Morphological And Molecular Approach, Konrad Mebert

Biological Sciences Theses & Dissertations

A few traditionally applied diagnostic characters of color pattern were compared with an additional set of morphological and genetic characters to evaluate differences between Nerodia sipedon and N. fasciata and to study the dynamics across their hybrid zone in the Carolinas. Many of the morphological characters exhibited significant interspecific differences, although only the number of dorsally complete crossbands (CBa) was diagnostic by itself. A discriminant function analysis of morphological characters was successful in separating both taxa. Species-specific nuclear markers, identified by the AFLP (Amplified Fragment Length Polymorphism) technique were nearly fixed and served as diagnostic markers. They revealed extensive introgression …

It Does Take A Village To Raise A Child, Rebecca J. Boyd

Sociology & Criminal Justice Theses & Dissertations

Utilizing Cullen's (1994) Social Support Theory and Hunter's (1985) three dimensions of social life, this study is a quantitative, cross-sectional secondary data analysis designed to examine the relationship between institutional social support and rates of juvenile crime for counties and cities in Virginia for the year 2000. Resting on the contention that an inverse, significant relationship exists between measures of institutional support and rates of juvenile property and violent crime, this study examines types of support provided by the institutions of family, school, and the government. These measures of institutional support include 1) familial support: median income, marriage support; 2) …

Male Reproductive Dynamics In The Caribbean Spiny Lobster Panulirus Argus, Jamie S. Heisig

Biological Sciences Theses & Dissertations

The population size structure of the Caribbean Spiny Lobster, Panulirus argus, in the Florida Keys, Florida (USA) has undergone a dramatic shift as a result of fishing pressure on the large individuals of the population. Male lobsters are affected most by fishing because of sexual dimorphism in size and other studies have shown that male size has direct impacts on reproductive output (i.e., number of fertilized eggs/clutch) that are independent of female size. To assess more precisely the impact of male size on fertilization success, I conducted laboratory experiments and field observations to examine various male reproductive attributes over …

Keys To The Mammals And Mammal Skulls Of The Northern Coastal Plain Of Virginia, John F. Pagels, A. Scott Bellows, Joseph C. Mitchell

Virginia Journal of Science

This publication is designed to function as a tool for the identification of mammal species that occur on the Coastal Plain of northern Virginia. We provide whole-body and skull keys to the 40 species that occur in this region. Baseline data for this work were collected during several studies conducted on Fort A.P. Hill, Caroline County. The intended audience includes interested naturalists, teachers, students, field biologists, and natural resource managers.

Adaptive Segmentation Techniques For Object Region Extraction From Images With Complex Lighting Environment, Deepthi P. Valaparla

Electrical & Computer Engineering Theses & Dissertations

Automated image segmentation of objects in complex lighting environments is a difficult task since it is not possible to pre-define a threshold to distinguish the object from the background. It is ascertained that an effective threshold citation for such objects depends on the negative binomial distribution of the object region with respect to the background lighting. Based on this concept, a novel adaptive threshold selection method for automatic segmentation of the lumen region in endoscopic images is presented in this thesis. More precise lumen region and boundary are obtained by an integrated neighborhood search based region growing procedure whose speed …

Expression Of Glutathione-S-Transferase Mu 1 (Gstm1) And Glutathione-S-Transferase Theta 1 (Gstt1) In Patients With Aplastic Anemia And Myelodysplastic Syndromes, Aaron Conrad Spivey

Theses and Dissertations in Biomedical Sciences

Aplastic Anemia is a disorder of the hematopoietic bone marrow stem cells that is frequently associated with drug or chemical exposures. Reduced ability to detoxify drugs or chemicals may result in an increased risk of disease. Glutathione S-transferases are Phase II enzymes, which help detoxify xenobiotics. Many patients who have developed aplastic anemia and myelodysplastic syndrome have the null genotype for GSTMI and/or GSTTI. For patients with GSTMI and GSTTI positive genotypes, non-expression of these enzymes may cause an increased risk in developing aplastic anemia or myelodysplastic syndrome. To test whether genotype positive patients have GSTMI or GSTTI null …

Real-Time Study Of Multidrug Resistance Mechanism In Pseudomonas Aeruginosa Using Nanoparticle Optics And Single Live Cell Imaging, Sophia Vasou Kyriacou

Chemistry & Biochemistry Theses & Dissertations

This thesis centers on the study of the xenobiotic efflux system in Pseudomonas aeruginosa, which is a ubiquitous bacterium. It resists many structurally and functionally diverse substrates due to expression of Mex-extrusion pumps, including MexAB-OprM, MexCD-OprJ, MexEF-OprN and MexXY-OprM systems. Despite extensive research, the structure and mechanism of multidrug resistance is unclear (1-9). For example, (i) how do MexA, MexB and OprM proteins assemble to extrude antibiotics? (ii) What is the antibiotic susceptibility of MexA, MexB, and OprM proteins? (iii) How do substrates cross the outer membrane of P. aeruginosa? (iv) Where are antibiotics accumulated inside the cell? This thesis …

Coherent Backscattering Of Light From An Ultra Cold Gas Of Rubidium-85 Atoms, Pasad B. Kulatunga

Physics Theses & Dissertations

This thesis reports on the experimental study of coherent radiative transport in an ultracold gas of ⁸⁵Rb atoms confined in a magneto-optic trap. Measurements are made of the polarization dependence of the spatial and spectral profile of light backscattered from the sample. The results shows an interferometric enhancement sensitive to coherent multiple scattering in the atomic gas, and strong variations with the polarization of the incident and detected light. Effects due to coherent enhancement of weak non-resonant transitions are also observed. Comparison of the measurements with realistic quantum Monte Carlo simulations of Kupriyanov, et al [1] yield very good …

Hyaluronate Lyase Gene Of Streptococcus Pyogenes: Molecular Characterization And Regulation, Sheryl Lynne Walton

Theses and Dissertations in Biomedical Sciences

Streptococcus pyogenes causes an assortment of diseases ranging from pharyngitis to necrotizing fasciitis. This bacterium has the ability to elaborate several extracellular products capable of causing tissue damage; one of which is a hyaluronate lyase. Little information is available regarding the regulation of streptococcal hyaluronidase. As such, the expression of hylA in the hyaluronate lyase-producing strain ATCC 10403 and in strains that do not produce detectable enzyme was analyzed by RT-PCR and quantitative PCR. Hyaluronate lyase transcript was detected throughout growth for all strains; however, expression of hylA in the enzymatically inactive strains, 71698 and SF370, was determined …

Science Comprehension: Effects Of Text Coherence And Reader Aptitudes, Kimberly G. Cottrell

Psychology Theses & Dissertations

There is a significant need to improve reading comprehension as typical textbooks, particularly science textbooks, are often too difficult for the average student to comprehend. The overall purpose of this study was to examine which reader aptitudes, specifically prior domain knowledge, reading ability, metacognitive reading strategy knowledge, and motivation, were most predictive of science text comprehension, and whether these relationships varied as a function of text coherence. Further, the purpose entailed which factors (reading ability, metacognitive reading strategy knowledge, and motivation) may assist low knowledge readers to compensate for knowledge deficits, particularly when reading low-coherence texts. There were 241 undergraduate …

Characterization Of Mouse Prostate Tumor-Infiltrating Leukocytes, Daniel Alan Holterman

Theses and Dissertations in Biomedical Sciences

Prostate cancer is the most common form of non-cutaneous cancer among men in the United States and the second most common cause of cancer-related death among men with approximately 189,000 new cases diagnosed and 30,200 deaths of the disease in 2002. Prostate cancer can be a treatable disease but once it becomes metastatic there are no acceptable therapies. For this reason, immunotherapy has been attempted but the results have been disappointing. The TRAMP model was used to evaluate specific interactions between an intact murine immune system and a prostate tumor that expressed a naturally processed weak tumor antigen. Vaccination trials …

The Ecological Ramifications Of Disease And Density In The Caribbean Spiny Lobster, Panulirus Argus, Donald C. Behringer Jr.

Biological Sciences Theses & Dissertations

In 1999, I discovered the first virus known to be pathogenic to any species of lobster. HLV-PA is a pathogenic herpes-like virus that infects juvenile Caribbean spiny lobster, Panulirus argus, in the waters off south Florida (USA), and it alters the behavior and ecology of this species in fundamental ways. Gross signs of HLV-PA infection are lethargy, morbidity, cessation of molting, and discolored, “milky” hemolymph that does not clot. HLV-PA infects the hemocytes of host lobsters, specifically the hyalinocytes and semi-granulocytes, but not the granulocytes. When hemolymph from infected donors was injected into healthy juvenile lobsters, 90% of the …

Clermont, Iowa: The Moon, Kyle Kuhn

English Theses & Dissertations

Although Clermont, Iowa is not defined in concrete terms, the stories give dimension to the town and make the reader feel as if he or she is a part of the community, overhearing a story at the grocery store or at the bowling alley. The stories work together to show the contradictions of rural life, the caring community that exists alongside the deceit and lies that drive many of its members.

The Quantitative Assessment Of Osteoinductivity Of Human Demineralized Bone Matrix And Cdna Array Analysis Of Osteogenic Differentiation In Human Periosteal Cells, Sittisak Honsawek

Theses and Dissertations in Biomedical Sciences

Demineralized bone matrix (DBM) has been extensively utilized in orthopeadic, periodontal, and maxillofacial applications. DBM comprises bone morphogenetic proteins (BMPs) that are essential regulators for endochondral bone formation. The current study was undertaken to establish the quantitative in vitro assays for determining the osteoinductive potential of human DBM and the use of cDNA gene expression array technology for examining gene expression profiles during osteoblast differentiation in human periosteal cells.

BMP-4 is one of the most osteoinductive factors and has been investigated for clinical applications. For this reason, BMP-4 was designated as the osteoinductive protein for use in the quantitative in …

Comparison Of Methods For Dna Isolation From Food Samples For Detection Of Shiga Toxin-Producing Escherichia Coli By Real-Time Pcr, Loree C. Heller, Carisa R. Davis, K. Kealy Peak, David Wingfield, Andrew C. Cannons, Philip T. Amuso, Jacqueline Cattani

Bioelectrics Publications

In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

Status And Trends In Water Quality And Living Resources In The Virginia Chesapeake Bay: James River (1985-2001), Daniel M. Dauer, Harold G. Marshall, Kent E. Carpenter, John R. Donat, Michael F. Lane

Status and Trends for Virginia Chesapeake Bay

This summary includes materials provided by Rick Hoffman of the Chesapeake Bay Program of the Virginia Department of Environmental Quality. Environmental information regarding other important conditions in Chesapeake Bay (e.g. submerged aquatic vegetation, fisheries, chemical contaminants) has been reported previously (Chesapeake Bay and its Tributaries: Results of Monitoring Programs And Status of Resources; 2002 Biennial Report of the Secretary of Natural Resources to The Virginia General Assembly).

The Virginia Chesapeake Bay and its tidal tributaries continue to show some environmental trends indicating progress toward restoration of a more balanced and healthy ecosystem. However, the Bay system remains degraded …

Status And Trends In Water Quality And Living Resources In The Virginia Chesapeake Bay: Rappahannock River (1985-2001), Daniel M. Dauer, Harold G. Marshall, Kent E. Carpenter, John R. Donat, Michael F. Lane

Status and Trends for Virginia Chesapeake Bay

This summary includes materials provided by Rick Hoffman of the Chesapeake Bay Program of the Virginia Department of Environmental Quality. Environmental information regarding other important conditions in Chesapeake Bay (e.g. submerged aquatic vegetation, fisheries, chemical contaminants) has been reported previously (Chesapeake Bay and its Tributaries: Results of Monitoring Programs And Status of Resources; 2002 Biennial Report of the Secretary of Natural Resources to The Virginia General Assembly).

The Virginia Chesapeake Bay and its tidal tributaries continue to show some environmental trends indicating progress toward restoration of a more balanced and healthy ecosystem. However, the Bay system remains degraded …