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Monarch, Jim Raper (Editor)

Monarch Magazine

Winter 2014 issue of Monarch, the Old Dominion University Magazine.

Subtitled: Nurturing Entrepreneurs

Krill, Climate, And Contrasting Future Scenarios For Arctic And Antarctic Fisheries, Margaret M. Mcbride, Padmini Dalpadado, Kenneth F. Drinkwater, Olav Rune Godø, Alistair J. Hobday, Anne B. Hollowed, Trond Kristiansen, Eugene J. Murphy, Patrick H. Ressler, Sam Subbey, Eileen E. Hofmann, Harald Loeng

CCPO Publications

Arctic and Antarcticmarine systems have incommon high latitudes, large seasonal changes in light levels, cold air and sea temperatures, and sea ice. In other ways, however, they are strikingly different, including their: age, extent, geological structure, ice stability, and foodweb structure. Both regions contain very rapidly warming areas and climate impacts have been reported, as have dramatic future projections. However, the combined effects of a changing climate on oceanographic processes and foodweb dynamics are likely to influence their future fisheries in very different ways. Differences in the life-history strategies of the key zooplankton species (Antarctic krill in the Southern Ocean …

Design And Study Of The Efflux Function Of The Egfp Fused Mexab-Oprm Membrane Transporter In Pseudomonas Aeruginosa Using Spectroscopy, Feng Ding, Kerry J. Lee, Ardeschir Vahedi-Faridi, Hiroshi Yoneyama, Christopher J. Osgood, Xiao-Hong Nancy Xu

Biological Sciences Faculty Publications

Multidrug membrane transporters (efflux pumps) can selectively extrude a variety of structurally and functionally diverse substrates (e.g., chemotoxics, antibiotics), leading to multidrug resistance (MDR) and ineffective treatment of a wide variety of diseases. In this study, we have designed and constructed a fusion gene (egfp-mexB) of N-terminal mexB with C-terminal egfp, inserted it into a plasmid vector (pMMB67EH), and successfully expressed it in the Δ MexB (MexB deletion) strain of Pseudomonas aeruginosato create a new strain that expresses MexA-(EGFP-MexB)-OprM. We characterized the fusion gene using gel electrophoresis and DNA sequencing, and determined its expression in live …

Philosophy, Medicine And Health Care - Where We Have Come From And Where We Are Going, Michael Loughlin, Robyn Bluhm, Jonathan Fuller, Stephen Burtow, Rose E. G. Upshur, Kirstin Borgerson, Maya J. Goldenberg, Elselijn Kingma

Philosophy Faculty Publications

The role of philosophy in discussions of clinical practice was once regarded by many as restricted to a very limited version of ‘medical ethics’, one that has been extensively criticized in the pages of this journal and elsewhere for being at once philosophically untenable and practically unhelpful [1–4]. While this uninspiring view of the nature and scope of applied philosophy has by no means been eradicated, over a number of years there has been a resurgence of interest in the philosophy of medicine and health care as an intellectually serious and practically significant enterprise. Controversies about evidence, value, clinical knowledge, …

Automated Identification Of Cell Type Specific Genes In The Mouse Brain By Image Computing Of Expression Patterns, Rongjian Li, Wenlu Zhang, Shuiwang Ji

Computer Science Faculty Publications

Background: Differential gene expression patterns in cells of the mammalian brain result in the morphological, connectional, and functional diversity of cells. A wide variety of studies have shown that certain genes are expressed only in specific cell-types. Analysis of cell-type-specific gene expression patterns can provide insights into the relationship between genes, connectivity, brain regions, and cell-types. However, automated methods for identifying cell-type-specific genes are lacking to date.

Results: Here, we describe a set of computational methods for identifying cell-type-specific genes in the mouse brain by automated image computing of in situ hybridization (ISH) expression patterns. We applied invariant image feature …

Tracing Beta Strands Using Strandtwister From Cryo-Em Density Maps At Medium Resolutions, Dong Si, Jing He

Computer Science Faculty Publications

Major secondary structure elements such as α helices and β sheets can be computationally detected from cryoelectron microscopy (cryo-EM) density maps with medium resolutions of 5–10 A˚ . However, a critical piece of information for modeling atomic structures is missing, because there are no tools to detect β strands from cryo-EM maps at medium resolutions. We propose a method, StrandTwister, to detect the traces of β strands through the analysis of twist, an intrinsic nature of a β sheet. StrandTwister has been tested using 100 β sheets simulated at 10 A˚ resolution and 39 β sheets computationally detected from cryo-EM …

Autologous Platelet Rich Plasma (Platelet Gel): An Appropriate Intervention For Salvaging Cardiac Myocytes Under Oxidative Stress After Myocardial Infarction, Barbara Y. Hargrave

Bioelectrics Publications

Background: The prompt restoration of blood flow (reperfusion) to the ischemic myocardium after an acute myocardial infarction is critical to the survival of non damaged heart tissue. However, reperfusion is responsible for additional myocardial damage. Our objective was to investigate the role of autologous platelet rich plasma or platelet gel prepared using nanosecond pulsed electric fields (nsPEFs) in improving left ventricular mechanical function after ischemic reperfusion.

Methods: The hearts of nine adult female New Zealand White rabbits were perfused using the Langendorff model. The hearts were exposed to global ischemia for 30 min and reperfused for 60 min. The hearts …

Microfluidic Electrical Sorting Of Particles Based On Shape In A Spiral Microchannel, John Dubose, Xinyu Lu, Saurin Patel, Shizhi Qian, Sang Woo Joo

Mechanical & Aerospace Engineering Faculty Publications

Shape is an intrinsic marker of cell cycle, an important factor for identifying a bioparticle, and also a useful indicator of cell state for disease diagnostics. Therefore, shape can be a specific marker in label-free particle and cell separation for various chemical and biological applications. We demonstrate in this work a continuous-flow electrical sorting of spherical and peanut-shaped particles of similar volumes in an asymmetric double-spiral microchannel. It exploits curvature-induced dielectrophoresis to focus particles to a tight stream in the first spiral without any sheath flow and subsequently displace them to shape-dependent flow paths in the second spiral without any …

Technological Literacy Courses In Pre-Service Teacher Education, Roger Skophammer, Philip A. Reed

STEMPS Faculty Publications

The goal of this study was to determine to what extent technological literacy courses were required in K-12 teacher education. A documents review of the appropriate course catalogs for initial teacher preparation was conducted. The documents review identified general education requirements and options for technological literacy courses, as well as requirements and options for these courses for English, social studies, mathematics, and science education majors. For this study, technological literacy was defined as "the ability to use, manage, assess, and understand technology" (ITEA, 2000/2002/2007, p. 9). This definition of literacy is broader than technology literacy associated with computer use and …

Perceptions Of The Counseling Profession: From Health Science Graduate Faculty And Students, Kaprea F. Johnson, Christianne Fowler, Karen Kott, Margaret Lemaster

Ellmer School of Nursing Faculty Publications

Counseling is considered a newer healthcare profession and possibly unknown to others. This study uses a post-positivist phenomenological approach exploring healthcare faculty and students' perceptions of counseling professionals after participating in a semester long interprofessional health promotions and maintenance course. Results indicated most professions are unaware of counselors, their scope of practice, and their benefit to interprofessional teams. Participants showed growth in their knowledge. Results indicate the importance of interprofessional education and need for advocacy awareness.

Exposure To Endocrine Disrupting Chemicals And Male Reproductive Health, Hueiwang Anna Jeng

Community & Environmental Health Faculty Publications

Endocrine disrupting chemicals (EDCs) can interfere with normal hormonal balance and may exert adverse consequences on humans. The male reproductive system may be susceptible to the effects of such environmental toxicants. This review discusses the recent progress in scientific data mainly from epidemiology studies on the associations between EDCs and male reproductive health and our understanding of possible mechanisms associated with the effects of EDCs on male reproductive health. Finally, the review provides recommendations on future research to enhance our understanding of EDCs and male reproductive health. The review highlights the need for (1) well-defined longitudinal epidemiology studies, with appropriately …

A Coupled Geochemical And Biogeochemical Approach To Characterize The Bioreactivity Of Dissolved Organic Matter From A Headwater Stream, Rachel L. Sleighter, Rose M. Cory, Louis A. Kaplan, Hussain A.N. Abdulla, Patrick G. Hatcher

Chemistry & Biochemistry Faculty Publications

The bioreactivity or susceptibility of dissolved organic matter (DOM) to microbial degradation in streams and rivers is of critical importance to global change studies, but a comprehensive understanding of DOM bioreactivity has been elusive due, in part, to the stunningly diverse assemblages of organic molecules within DOM. We approach this problem by employing a range of techniques to characterize DOM as it flows through biofilm reactors: dissolved organic carbon (DOC) concentrations, excitation emission matrix spectroscopy (EEMs), and ultrahigh resolution mass spectrometry. The EEMs and mass spectral data were analyzed using a combination of multivariate statistical approaches. We found that 45% …

Wavelength And Temperature-Dependent Apparent Quantum Yields For Photochemical Formation Of Hydrogen Peroxide In Seawater, David J. Kieber, Gary W. Miller, Patrick J. Neale, Kenneth Mopper

Chemistry & Biochemistry Faculty Publications

Wavelength and temperature-dependent apparent quantum yields (AQYs) were determined for the photochemical production of hydrogen peroxide using seawater obtained from coastal and oligotrophic stations in Antarctica, the Pacific Ocean at Station ALOHA, the Gulf of Mexico, and at several sites along the East Coast of the United States. For all samples, AQYs decreased exponentially with increasing wavelength at 25 °C, ranging from 4.6 × 10−4 to 10.4 × 10−4 at 290 nm to 0.17 × 10−4 to 0.97 × 10−4 at 400 nm. AQYs for different seawater samples were remarkably similar irrespective of expected differences in the composition and concentrations …

Synthesis And Characterization Of Ph Responsive D-Glucosamine Based Molecular Gelators, Navneet Goyal, Hari P.R. Mangunuru, Bargav Parikh, Sonu Shrestha, Guijun Wang

Chemistry & Biochemistry Faculty Publications

Small molecular gelators are a class of compounds with potential applications for soft biomaterials. Low molecular weight hydrogelators are especially useful for exploring biomedical applications. Previously, we found that 4,6-O-benzylidene acetal protected D-glucose and D-glucosamine are well-suited as building blocks for the construction of low molecular weight gelators. To better understand the scope of D-glucosamine derivatives as gelators, we synthesized and screened a novel class of N-acetylglucosamine derivatives with a p-methoxybenzylidene acetal protective group. This modification did not exert a negative influence on the gelation. On the contrary, it actually enhanced the gelation tendency for many derivatives. The …

Derivation Of Tropospheric Methane From Tccon Ch4 And Hf Total Column Observations, K. M. Saad, D. Wunch, P. Bernath, C. Boone, B. Connor, N. M. Deutscher, D.W.T. Griffith, R. Kivi, J. Notholt, C. Roehl, M. Schneider, V. Sherlock, P. O. Wennberg

Chemistry & Biochemistry Faculty Publications

The Total Carbon Column Observing Network (TCCON) is a global ground-based network of Fourier transform spectrometers that produce precise measurements of column-averaged dry-air mole fractions of atmospheric methane (CH₄). Temporal variability in the total column of CH₄ due to stratospheric dynamics obscures fluctuations and trends driven by tropospheric transport and local surface fluxes that are critical for understanding CH₄ sources and sinks. We reduce the contribution of stratospheric variability from the total column average by subtracting an estimate of the stratospheric CH₄ derived from simultaneous measurements of hydrogen fluoride (HF). HF provides a proxy for …

Anomia Treatment Platform As Behavioral Engine For Use In Research On Physiological Adjuvants To Neurorehabilitation, Diane Kendall, Anastasia Raymer, Miranda Rose, Joellen Gilbert, Leslie J. Gonzalez Rothi

Communication Disorders & Special Education Faculty Publications

The purpose of this study was to create a "behavioral treatment engine" for future use in research on physiological adjuvants in aphasia rehabilitation. We chose the behavioral target anomia, which is a feature displayed by many persons who have aphasia. Further, we wished to saturate the treatment approach with many strategies and cues that have been empirically reported to have a positive influence on aphasia outcome, with the goal being to optimize the potential for positive response in most participants. A single-subject multiple baseline design with replication across eight participants was employed. Four men and four women, with an average …

Multiplexing With Three-Primer Pcr For Rapid And Economical Microsatellite Validation, Salla Vartia, Patrick C. Collins, Thomas F. Cross, Richard D. Fitzgerald, David T. Gauthier, Philip Mcginnity, Luca Mirimin, Jens Carlsson

Biological Sciences Faculty Publications

The next generation sequencing revolution has enabled rapid discovery of genetic markers, however, development of fully functioning new markers still requires a long and costly process of marker validation. This study reports a rapid and economical approach for the validation and deployment of polymorphic microsatellite markers obtained from a 454 pyrosequencing library of Atlantic cod, Gadus morhua, Linnaeus 1758. Primers were designed from raw reads to amplify specific amplicon size ranges, allowing effective PCR multiplexing. Multiplexing was combined with a three-primer PCR approach using four universal tails to label amplicons with separate fluorochromes. A total of 192 primer pairs …

Global Priorities For Marine Biodiversity Conservation, Elizabeth R. Selig, Will R. Turner, Sebastian Troëng, Bryan P. Wallace, Kristin Kaschner, Ben G. Lascelles, Kent E. Carpenter, Russell A. Mittermeier

Biological Sciences Faculty Publications

In recent decades, many marine populations have experienced major declines in abundance, but we still know little about where management interventions may help protect the highest levels of marine biodiversity. We used modeled spatial distribution data for nearly 12,500 species to quantify global patterns of species richness and two measures of endemism. By combining these data with spatial information on cumulative human impacts, we identified priority areas where marine biodiversity is most and least impacted by human activities, both within Exclusive Economic Zones (EEZs) and Areas Beyond National Jurisdiction (ABNJ). Our analyses highlighted places that are both accepted priorities for …

Modelling Cholera In Periodic Environments, Drew Posny, Jin Wang

Mathematics & Statistics Faculty Publications

We propose a deterministic compartmental model for cholera dynamics in periodic environments. The model incorporates seasonal variation into a general formulation for the incidence (or, force of infection) and the pathogen concentration. The basic reproduction number of the periodic model is derived, based on which a careful analysis is conducted on the epidemic and endemic dynamics of cholera. Several specific examples are presented to demonstrate this general model, and numerical simulation results are used to validate the analytical prediction.

Ehrlichia And Spotted Fever Group Rickettsiae Surveillance In Amblyomma Americanum In Virginia Through Use Of A Novel Six-Plex Real-Time Pcr Assay, David N. Gaines, Darwin J. Operario, Suzanne Stroup, Ellen Stromdahl, Chelsea Wright, Holly Gaff, James Broyhill, Joshua Smith, Douglas E. Norris, Tyler Henning, Agape Lucas, Eric Houpt

Biological Sciences Faculty Publications

The population of the lone star tick Amblyomma americanum has expanded in North America over the last several decades. It is known to be an aggressive and nondiscriminatory biter and is by far the most common human-biting tick encountered in Virginia. Few studies of human pathogen prevalence in ticks have been conducted in our state since the mid-twentieth century. We developed a six-plex real-time PCR assay to detect three Ehrlichia species (E. chaffeensis, E. ewingii, and Panola Mountain Ehrlichia) and three spotted fever group Rickettsiae (SFGR; R. amblyommii, R. parkeri, and R. rickettsii …

Hidden Diversity In Sardines: Genetic And Morphological Evidence For Cryptic Species In The Goldstripe Sardinella, Sardinella Gibbosa (Bleeker, 1849), Rey C. Thomas, Demian A. Willette, Kent E. Carpenter, Mudjekeewis D. Santos

Biological Sciences Faculty Publications

Cryptic species continue to be uncovered in many fish taxa, posing challenges for fisheries conservation and management. In Sardinella gibbosa, previous investigations revealed subtle intra-species variations, resulting in numerous synonyms and a controversial taxonomy for this sardine. Here, we tested for cryptic diversity within S. gibbosa using genetic data from two mitochondrial and one nuclear gene regions of 248 individuals of S. gibbosa, collected from eight locations across the Philippine archipelago. Deep genetic divergence and subsequent clustering was consistent across both mitochondrial and nuclear markers. Clade distribution is geographically limited: Clade 1 is widely distributed in the central Philippines, while …

Basin Isolation And Oceanographic Features Influencing Lineage Divergence In The Humbug Damselfish (Dascyllus Aruanus) In The Coral Triangle, J. M. Raynal, E. D. Crandall, P. H. Barber, G. N. Mahardika, M. C. Lagman, Kent E. Carpenter

Biological Sciences Faculty Publications

The Coral Triangle is a hotspot for marine species diversity as well as for intraspecific genetic diversity. Here, we used nuclear RAG2 and mitochondrial D-Loop genes to identify deep genetic divergence among Dascyllus aruanus (Linnaeus, 1758) populations across relatively short scales within the Coral Triangle. Mitochondrial clades different by >20 mutational steps were completely isolated from one another across the distance between Java and the Lesser Sunda Islands, and also showed frequency differences in the eastern and western Philippines. Evidence for population structure in the Sulu Sea and at the Lesser Sunda Islands was also identified. Our results suggest that …

Quantitative Factors Proposed To Influence The Prevalence Of Canine Tick-Bourne Disease Agents In The United States, Roger W. Stich, Byron L. Blagburn, Dwight D. Bowman, Christopher Carpenter, M. Roberto Cortinas, Sidney A. Ewing, Desmond Foley, Holly Gaff, Graham J. Hickling, R. Ryan Lash

Biological Sciences Faculty Publications

The Companion Animal Parasite Council hosted a meeting to identify quantifiable factors that can influence the prevalence of tick-borne disease agents among dogs in North America. This report summarizes the approach used and the factors identified for further analysis with mathematical models of canine exposure to tick-borne pathogens.

So, You Want To Use Next Generation Sequencing In Marine Systems? Insight From The Pan Pacific Advanced Studies Institute, D. A. Willette, F. W. Allendorf, P. H. Barber, D. J. Barshis, K. E. Carpenter, E. D. Crandall, W. A. Cresko, I. Fernandez-Silva, M. V. Matz, E. Meyer, M. D. Santos, L. W. Seeb, J. E. Seeb

Biological Sciences Faculty Publications

The emerging field of next-generation sequencing (NGS) is rapidly expanding capabilities for cutting edge genomic research, with applications that can help meet marine conservation challenges of food security, biodiversity loss, and climate change. Navigating the use of these tools, however, is complex at best. Furthermore, applications of marine genomic questions are limited in developing nations where both marine biodiversity and threats to marine biodiversity are most concentrated. This is particularly true in Southeast Asia. The first Pan-Pacific Advanced Studies Institute (PacASI) entitled "Genomic Applications to Marine Science and Resource Management in Southeast Asia" was held in July 2012 in Dumaguete, …

Comparison Of Phenology And Pathogen Prevalence, Including Infection With The Ehrlichia Muris-Like (Eml) Agent, Of Ixodes Scapularis Removed From Soldiers In The Midwestern And Northeastern United States Over A 15 Year Period (1997-2012), Ellen Stromdahl, Sarah Hamer, Sarah Jenkins, Lynne Sloan, Phillip Williamson, Erik Foster, Robyn Nadolny, Chad Elkins, Mary Vince, Bobbi Pritt

Biological Sciences Faculty Publications

Background: Since 1997, human-biting ticks submitted to the Department of Defense Human Tick Test Kit Program (HTTKP) of the US Army Public Health Command have been tested for pathogens by PCR. We noted differences in the phenology and infection prevalence among Ixodes scapularis ticks submitted from military installations in different geographic regions. The aim of this study was to characterize these observed differences, comparing the phenology and pathogen infection rates of I. scapularis submitted from soldiers at two sites in the upper Midwest ( Camp Ripley, MN, and Ft. McCoy, WI) and one site in the northeastern US (Ft. Indiantown …

Host Behavior Alters Spiny Lobster-Viral Disease Dynamics: A Simulation Study, Thomas W. Dolan Iii, Mark J. Butler Iv, Jeffrey D. Shields

Biological Sciences Faculty Publications

Social behavior confers numerous benefits to animals but also risks, among them an increase in the spread of pathogenic diseases. We examined the trade-off between risk of predation and disease transmission under different scenarios of host spatial structure and disease avoidance behavior using a spatially explicit, individual-based model of the host pathogen interaction between juvenile Caribbean spiny lobster (Panulirus argus) and Panulirus argus Virus 1 (PaV1). Spiny lobsters are normally social but modify their behavior to avoid diseased conspecifics, a potentially effective means of reducing transmission but one rarely observed in the wild. We found that without lobster avoidance of …

Phylogeography Unplugged: Comparative Surveys In The Genomic Era, Brian W. Bowen, Kartik Shanker, Nina Yasuda, Maria Celia Malay, Sophie Von Der Heyden, Gustav Paulay, Luiz A. Rocha, Kimberly A. Selkoe, Paul H. Barber, Suzanne T. Williams, Harilaos A. Lessios, Eric D. Crandall, Giacomo Bernardi, Christopher P. Meyer, Kent E. Carpenter, Robert J. Toonen

Biological Sciences Faculty Publications

In March 2012, the authors met at the National Evolutionary Synthesis Center (NESCent) in Durham, North Carolina, USA, to discuss approaches and cooperative ventures in Indo-Pacific phylogeography. The group emerged with a series of findings: (1) Marine population structure is complex, but single locus mtDNA studies continue to provide powerful first assessment of phylogeographic patterns. (2) These patterns gain greater significance/power when resolved in a diversity of taxa. New analytical tools are emerging to address these analyses with multi-taxon approaches. (3) Genome-wide analyses are warranted if selection is indicated by surveys of standard markers. Such indicators can include discordance between …

Eat It: Sex, Food And Women's Writing [Book Review], Marc Ouellette

English Faculty Publications

Simply put, Eat It: Sex, Food and Women's Writing surpasses its rather immodest claims. This is no mean feat, for the editors allow that they have collected short stories, nonfiction shorts and poetry that, as the back claims offers, hinge "on the carnal." More than that, the gathered works purportedly address the ways in which experiencing food entails nothing short of "power, biology, social obligation, experimentation, nourishment, pain and pleasure." The authors treat the topics, ranging from the politics of potatoes to tricks for field dressing deer, with a blend of seriousness and humour befitting the material. What becomes clear …

Evolution Of The Freshwater Sardinella, Sardinella Tawilis (Clupeiformes: Clupeidae), In Taal Lake, Philippines And Identification Of Iits Marine Sister-Species, Sardinella Hualiensis, Demian Willette, Kent E. Carpenter, Mudjekeewis Santos

Biological Sciences Faculty Publications

We identify the sister species of the world's only freshwater sardinella, Sardinella tawilis (Herre, 1927) of Taal Lake, Philippines as the morphologically-similar marine Taiwanese sardinella Sardinella hualiensis (Chu and Tsai, 1958). Evidence of incomplete lineage sorting and a species tree derived from three mitochondrial genes and one nuclear gene indicate that S. tawilis diverged from S. hualiensis in the late Pleistocene. Neutrality tests, mismatch distribution analysis, sequence diversity indices, and species tree analysis indicate populations of both species have long been stable and that the divergence between these two lineages occurred prior to the putative 18th century formation of Taal …

[Review Of The Book Rare Birds Of North America, By N.G. Howell, Ian Lewington & Will Russell], Natasha D.G. Hagemeyer, Eric L. Walters

Biological Sciences Faculty Publications

No abstract provided.