Digital Commons Network^™

Recombinant Gelonin: Purification And Functional Validation For Electroporation-Assisted Delivery, Eleni Zivla, Giedrė Šilkūnienė, Andrei G. Pakhomov, Mantas Šilkūnas, Olga N. Pakhomova

Knowledge and Creativity Expo

Type I ribosome-inactivating proteins, such as gelonin, are enzymatically potent toxins but are effectively non-toxic under physiological conditions because they are unable to cross the plasma membrane[1]. Pulsed electric fields (PEF) overcome this delivery barrier by transiently permeabilizing the plasma membrane, allowing direct cytosolic entry. This enhances gelonin cytotoxicity by several thousand-fold, highlighting gelonin’s potential as an electrochemotherapy candidate[2]. Here, we present a recombinant gelonin (rGel) expression platform in E. coli with HPLC-based purification that enables reproducible protein production and structure-guided engineering.

The mature gelonin sequence (residues 47–297) was cloned into pET-32a(+) with an N-terminally fused Trx-6×His tag and TEV …

Comparative Effect Of Mineral, Organic, And Mixed Sunscreens Formulations On The Life History Of A Benthic Marine Invertebrate, Jacob Anthony Fox, Courtney Lott, Araceli Rodriguez-Romero, Gloria Massamba N'Siala

Knowledge and Creativity Expo

Mineral and organic sunscreens are emerging anthropogenic pollutants in coastal marine waters and have been shown to affect marine invertebrates via genotoxicity, endocrine disruption, oxidative stress, and impacts on life-history traits, such as reduced survival, growth, and fecundity. Most experiments expose marine organisms to isolated UV filters, with fewer studies testing complete formulations, co-occurring exposure to organic and mineral sunscreens, or the modulating influence of format type on toxicity. In this experiment, we tested the impact of organic sunscreens (3% Avobenzone, 15% Homosalate, 5% Octinoxate, and 10% Octocylene) and 24% zinc oxide mineral sunscreens on the survival and fecundity of …

Effects Of Alk2 Deficiency On T Cell Fate And Regulatory T Cell Populations, Jalyn Reeves, Afia Salsabil Alam

Knowledge and Creativity Expo

Regulatory T cells (Tregs) are central to immune tolerance, and their lineage stability is established during thymic development and maintained in the periphery through tightly regulated signaling networks. Members of the BMP receptor family have been implicated in T-cell differentiation, yet the specific role of the type I receptor ALK2 in shaping cell fate and sustaining the regulatory program remains unclear. To examine how ALK2 contributes to T-cell development, we generated ALK2-knockout murine chimeras and analyzed lymphoid populations from the thymus, spleen, and peripheral tissues. Thymocytes and lymphocytes were isolated and stained with antibody panels to identify developmental subsets and …

Predator-Prey Responses Of The Clamworm Alitta Succinea To Direct Interaction With Crabs And Chemical Cues, Shyann Mcclain, Gloria M. Massamba N'Siala

Knowledge and Creativity Expo

Predator–prey interactions in marine benthic systems can have important indirect effects mediated through environmental signaling. Predators can reveal their presence through the release of chemical cues, influencing the activity and feeding rates of other potential prey through non-consumptive effects, which may ultimately shape energy allocation and survival in targeted populations. Along the Atlantic coast of the United States, predatory crabs overlap spatially with polychaete prey such as the clamworm Alitta succinea, creating consistent predation pressure. This study will set up the experimental system needed to examine the physiological responses of clamworms to both direct and indirect predation cues under controlled …

Seasonal Activity Pattern Of Ambrosia Beetles In Southeast Virginia, Umme H. Akter, Sajrim Chowdhury, Deborah A. Waller

Knowledge and Creativity Expo

Ambrosia beetles (Curculionidae: Scolytinae) are wood-boring insects that influence forest ecosystems through fungal symbioses, rapid host colonization, and invasive species dynamics. Despite their ecological importance, seasonal activity patterns remain poorly characterized in southeastern Virginia. This study integrates field trapping and statistical modeling to examine species-specific seasonal activity across two forested sites in Norfolk, Virginia. Beetles were monitored using ethanol and essential oil-baited bottle traps alongside ethanol-soaked bolt traps. Overall abundance peaked in April, indicating strong early-season emergence. Ethanol-baited traps captured 3-7 times more beetles than essential oil traps, and negative binomial generalized linear models confirmed bait type as a significant …

Protocol For Egg Mass Preservation And Its Efficacy In The Marine Annelids Ophryotrocha Labronica, Brennan Sinclair, Gloria Massamba-N'Siala

Knowledge and Creativity Expo

Accurate measurement of egg size is essential for understanding reproductive investment and developmental patterns in Ophryotrocha labronica, but standard protocols require imaging fresh eggs, limiting sample throughput. This study tested whether short‑term preservation in 4% formalin alters egg size and developed a workflow to support more efficient developmental observations. Egg masses were divided into subsamples and measured after 0, 2, and 7 days of preservation. Using a linear mixed‑effects model, we analyzed 224 eggs from 25 masses to evaluate the effect of preservation time while accounting for variation among clutches. Preservation time had no significant effect on egg size, …

Methods Of Inducing Cellular Senescence In Lung Fibroblasts, Sara Palega, Jennifer Q. Zhou, Shan Zhu, Yan Y. Sanders

Knowledge and Creativity Expo

Methods of Inducing Cellular Senescence in Lung Fibroblasts

Sara Palega¹, Jennifer Q Zhou², Shan Zhu², Yan Y Sanders²

¹VHS Biomedical Sciences PhD program, ²Department of Biomedical and Translational Sciences, Macon & Joan Brock Virginia Health Sciences at Old Dominion University (ODU)

Introduction: Idiopathic Pulmonary Fibrosis (IPF) is the most prevalent and severe of interstitial lung diseases. This chronic and progressive condition lacks treatment options and is ultimately fatal without a lung transplant. IPF arises from aberrant wound healing that leads to excessive collagen deposition, inflammation, and cystic alveolar growths which makes …

Methane Consumption Rates By Coastal Virginia Beach Microbes, James R. Bailey Ii

Knowledge and Creativity Expo

This study measures whether microbial communities in the shallow coastal waters of Virginia Beach are capable of consuming methane in the water column. Using methane enrichment incubations and gas chromatography, we found no measurable methane oxidation of our samples over multi-day periods. These results suggest that these coastal waters may act as net sources of atmospheric methane.

Using Spices To Mitigate The Detrimental Effects Of The Red Imported Fire Ant Solenopsis Invicta Buren (Formicidae) In Virginia, Karson Mchendry, Deborah Waller

Knowledge and Creativity Expo

The Red Imported Fire Ant, Solenopsis Invicta Buren, first arrived in southeastern Virginia in 1989 and rapidly spread throughout the area. These invasive ants displace native ant species and prey on vulnerable invertebrate and vertebrate species. Fire ant workers are very aggressive and readily bite and sting humans. Although the ant colonies can be eliminated with pesticide applications, chemical control can have detrimental effects on humans and on the environment. In the present study, we investigated various spices including cinnamon, nutmeg and turmeric as repellents to foraging workers. The use of spices to control ant activity promises to be an …

Trapping Ambrosia Beetles (Curculionidae) With Ethanol-Soaked Tree Bolts In Southeastern Virginia., Sajrim Chowdhury, Umme H. Akter, Deborah A. Waller

Knowledge and Creativity Expo

Native and invasive ambrosia beetles infest a wide variety of tree species in southeastern Virginia and cause significant damage. We investigated trapping ambrosia beetles by using ten different tree species as lures. One objective of the study was to determine the most effective lure for each species of ambrosia beetle related to tree species and the diameter of the bolt. Sections of tree limbs of two different diameters and 25cm in length were soaked in ethanol and hung from trees in several natural locations, both near water and away from water. Beetles attracted to the lures bored into the bolts …

Establishing Aquarium Systems And Care Protocols For Marine Invertebrates In The Department Of Biological Sciences At Odu, Magdalene R. Hamlin, Gloria Massamba N'Siala Ph.D.

Knowledge and Creativity Expo

Maintaining live animals in lab settings is useful for establishing a stock of specimens available for experimental studies without relying on continuous collection efforts. Previous aquarium systems utilized in the aquatic facility of the Department of Biological Sciences at ODU were suited for research on freshwater and marine vertebrates and were not designed for the care of marine invertebrates. This project aims to establish methods and protocols suitable for maintaining various marine invertebrates in a control laboratory setting. In the first stage of the project, the existing recirculation system and lab space will be reorganized and restructured to suit the …

The Influence Of Wind Speed On Phytoplankton Bloom Timing In Antarctica's 5 Regions, Majesti L. Cummings, Jessie S. Turners

Knowledge and Creativity Expo

Phytoplankton blooms form the foundation of the Antarctic marine food web and play a critical role in regional carbon cycling. Recent observations along the West Antarctic Peninsula indicate shifts in bloom phenology, the seasonal timing of biological events in nature, with accumulation seasons occurring later in recent decades. This study investigates how different variability in spring wind speed influences phytoplankton bloom start dates and peak dates across five ecological regions of the West Antarctic Peninsula.

Bloom timing is derived from 25 years of satellite-based chlorophyll-a observations, including annual start date and peak date. Wind speed data derived from atmospheric reanalysis …

How Much Does Shape Matter: Investigating The Impact Of Marine Particle Morphological Features On In-Situ Settling Velocities Using Pca And Various Ml Models, Huanqing Huang, Alexander B. Bochdansky

Knowledge and Creativity Expo

Particle settling velocity serves as an essential component in ocean biological pump, as it determines particle retention time in the water column. Stokes’ law has been widely used to predict particle settling velocities by particle size and excess density in aquatic environments. However, an increasing number of studies suggest that Stokes’ law fits poorly in the size-velocity relationship of observations on small oceanic particles. Here, we present a series of novel approaches to investigate the relative contribution of settling velocities by the particle shape and optical densities using machine learning (ML) models and principal component analysis (PCA), based on 3906 …

Mitochondrial Transfer And Induced Ros As A Therapeutic Strategy In Idh2-Mutant Chondrosarcoma, Vegas R. Bedder, Caleb Wyckoff, Chris Osgood, Gavin A. Vasquez, Michael Stacey

Knowledge and Creativity Expo

Chondrosarcoma (CS) are common bone cancers that produce cartilaginous tumors and are extremely refractive to chemo-and-radiation therapies, leaving very limited treatment options. They contain mutations in the isocitrate dehydrogenase genes, IDH1 or IDH2. Either IDH1 or IDH2 mutations are present in individual tumors, but not both. They both convert alphaketoglutarate (αKG) into the potent oncometabolite D-2-hydroxyglutarate (D2HG). D2HG can be transported from the tumors to cells of the tumor microenvironment (TME) where it can alter metabolic and epigenetic profiles in recipient cells. The process of α-KG to D2HG conversion occurs in the cytoplasm of IDH1 mutant cells, and in the …

Human Subject Studies For The Alignment Of Llm-As-A-Judge Evaluation Metric For Science News, Gabriel Vega Osborne

Knowledge and Creativity Expo

Science news has become an important vehicle to disseminate scientific breakthroughs, discoveries, and technological innovations. With the advancement of large language models and related AI models, it is possible to automatically generate science news from scientific papers, extending the reader population from domain scientists to a broader scope. However, how to evaluate the quality of the generated news warrants research. Traditional token based metrics have been shown to fail to evaluate the semantics and nuances of science news. Inspired by the fact that a major goal of science news is to educate readers with new knowledge, we thus propose knowledge …

Who Feeds On Whom? Identifying Ticks Feeding On Mammal, Brenna G. Albright, Christina Espada, Mykenna Zettle, Abbey Jones, David T. Gauthier, Holly Gaff

Knowledge and Creativity Expo

Study of tick-host association is critical to understanding host-pathogen ecology. Collection of ticks from mammalian hosts is difficult because of permitting, cost, and time considerations, particularly for medium and large mammals. The ODU Tick Research Team has been collecting ticks from mammals since 2010 using a combination of serendipitous sampling from roadkill and donations from veterinarians, hunters, and wildlife rehabilitators. In this work, we quantify the association between Ixodes scapularis and Ixodes keiransi and various mammalian hosts in Virginia. Ixodes scapularis (deer tick or blacklegged tick) is the primary vector of the Lyme disease bacterium (Borrelia burgdorferi s.s.) in …

The Phoenix Trauma Response Model (Ptrm): An Integrative And Innovative Approach To Trauma Recovery And Growth, Latesha S. Holloman, Lauren B. Robins, Tammie Willis, Courtney V. Morris

Journal of Human Services Scholarship and Interprofessional Collaboration

Trauma is increasingly understood as a developmentally consequential experience that shapes identity, regulation, and relational functioning across the lifespan. Although trauma-informed care, resilience theory, posttraumatic growth, and narrative approaches have expanded understanding of recovery, few frameworks articulate a structured developmental progression that integrates prevention, intervention, and intergenerational transformation within a unified model. The Phoenix Trauma Response Model (PTRM) addresses this gap by introducing a cyclical, phase-based framework organized around four interrelated processes: Recognition, Reframing, Reclamation, and Replication of Strength. Central to the model is the construct of conversion potential, defined as the structured capacity to translate distress into purposeful …

Aquatic Interrelations In Malika Mokeddem’S Novel N’Zid (2001), Laura C. Klein

Green Humanities: A Journal of Ecological Thought in Literature, Philosophy & the Arts

This article offers an ecological analysis of Malika Mokeddem's novel N'Zid (2001), moving beyond postcolonial and anthropocentric interpretations. It centers on the protagonist Nora's individuation, arguing this process is intrinsically linked to her evolving relationship with the aquatic environment from which she emerges. The article aims to situate N'Zid within an ecological perspective, particularly relevant in the Anthropocene, an era compelling a reassessment of humanity's place. Echoing Bruno Latour, addressing inter-relationality is essential as the stable physical framework of the Moderns becomes unstable. This view necessitates rethinking environmental issues and encourages a scientific interpretation of the novel, drawing on neurosciences, …

In The Science Library, Madeleine Wattenberg

Green Humanities: A Journal of Ecological Thought in Literature, Philosophy & the Arts

No abstract provided.

Volume 5 - Water (Full)

Green Humanities: A Journal of Ecological Thought in Literature, Philosophy & the Arts

No abstract provided.

Critical Design And The Robotic Imaginary: Design Criticism And Critical Robots, Jörgen Behrendtz

The Journal of Sociotechnical Critique

This paper brings critical design and design criticism to critical robot studies. Critical design can be described as an umbrella term for approaches making use of speculative designs to highlight and challenge narrow assumptions, preconceptions or even taken-for-granted truths about the role products play (in a wide sense) in everyday life. Design criticism refers to a reasoned procedure that investigates emancipatory and/or limiting potentials of designs, theorizes the roles of affordances, materials and embodied perception as epistemological resources in media interaction, and clarifies connections between specific design choices and their associated experiential qualities, seductive powers, ideological underpinnings, material operations, and …

A Case Of Listeria Monocytogenes Bacteremia In A Middle-Aged Woman With A History Of Primary Biliary Cholangitis, Caleb Smack, Jared T. Lausen, Aaron Mills

Eastern Virginia Medical Journal

In this report, we discuss the case of a patient with Listeria monocytogenes bacteremia likely resulting from primary biliary cholangitis (PBC) induced cirrhosis. We aim to bolster the body of literature on Listeria bacteremia and establish PBC as a risk factor for this rare complication. Our patient, who presented with sepsis, was subsequently found to have Listeria bacteremia and received intravenous antibiotic treatment of ampicillin for 3 total weeks with complete resolution of her disease. This is the first case suggesting that cirrhosis as a result of PBC may be an independent risk factor for Listeria bacteremia. In patients at …

A. Vs. I In Ai: Is There A Threshold To "Engineered" Intelligence?, Joshit Mohanty

Engineering Management & Systems Engineering Faculty Publications

Despite artificial intelligence reshaping the world, its development generates uncertainties regarding future capabilities. AI simultaneously exists as an artifact of engineering design and as autonomous intelligence, creating an observer-participant feedback loop. This paper proposes that embodied AI faces a bandwidth-limited intelligence threshold T_h that it arises from B = min(C_sens,C_Act). However, Shannon capacity measures bits while intelligence operates on concepts, necessitating a dual-channel model separating physical bandwidth B_io from representational capacity B_rep. Intelligence emerges as multi-dimensional rather than scalar, with components exhibiting different bandwidth dependencies. Surpassing T_h requires either new sensing methods expanding B, enhanced representational frameworks, or reconceptualization within …

Part Ii: Industrial Information Integration Review 2020-2025, Jinzhi Li

Information Technology & Decision Sciences Faculty Publications

Industrial Information Integration Engineering (IIIE) has become increasingly essential for improving operational efficiency and harmonizing heterogeneous industrial systems through advanced digital integration approaches. Fueled by rapid advancements in Industry 4.0 technologies—including digital twins, artificial intelligence, immersive interfaces, and IoT infrastructures—IIIE is substantially transforming traditional enterprise architecture and integration frameworks. This systematic review synthesizes recent developments and emerging trends, with particular attention to the accelerating adoption of digital twins and the deepening convergence between operational technologies (OT) and information technologies (IT) across multiple sectors. While notable progress has been made, significant challenges persist, especially in developing resilient integration architectures and fully …

Legislative Debate-Attributed Suicidality Among Lgbtq+ Adults: The Buffering Effect Of Community Belongingness, Keith J. Watts, Shawndaya S. Thrasher, Laneshia R. Conner, Nicole Campbell, Louis G. Baser, Dekeitra Griffin, Sydney P. Howard, Missy Spears, Justin X. Moore

Counseling & Human Services Faculty Publications

Background: In recent years, the sociopolitical landscape in the United States has shifted due to an increase in state-level legislation regarding LGBTQ+ rights, a trend that has been particularly pronounced in the Commonwealth of Kentucky. While the mental health impacts of enacted laws are increasingly documented, a critical gap remains in understanding the psychological toll of the legislative debates themselves—the prolonged periods of public discourse surrounding the restriction of rights. Methods: Utilizing data from the 2025 Queer Kentucky Survey (N = 817), this exploratory study examined the association between LGBTQ+ community belongingness and acute suicidality attributed specifically to anti-LGBTQ+ legislative …

Sleepiness In Pregnancy And Adverse Perinatal Outcomes, Rachel Friedlander, Xiaoning Huang, Phyllis Zee, Sadiya S. Khan, Philip Greenland, Francesca L. Facco, Judith Chung, William A. Grobman, Shalini Manchanda, Uma M. Reddy, George R. Saade, Robert M. Silver, Lynn M. Yee

Department of Obstetrics & Gynecology Faculty Publications

Background

Sleep disorders in pregnancy have been associated with adverse pregnancy outcomes including hypertensive disorders of pregnancy, preterm birth, and gestational diabetes mellitus, yet these findings are largely from retrospective studies. Using data from a prospective study that used validated tools to assess sleepiness in pregnancy, we aimed to assess the association of sleepiness in pregnancy with adverse pregnancy outcomes.

Methods

In this secondary analysis of data from a large, diverse prospective cohort of nulliparous individuals conducted at eight United States medical centers, those with self-reported sleep data were included. Sleepiness was evaluated in early (6-13 weeks) and mid-pregnancy (22-29 …

Proof-Of-Concept Development And Preclinical Evaluation Of A Microarray Patch Platform For Codelivery Of Multiple Broadly Neutralizing Antibodies For Hiv Prevention, M. Melissa Peet, Vivek Agrahari, Parbeen Singh, Lam Luong Hoai Nguyen, Louise A. Ouattara, Eun Sung Yang, Carolina Herrera, Amarendra Pegu, Theodore C. Pierson, Thanh D. Nguyen, Gustavo F. Doncel

CONRAD Publications

Broadly neutralizing antibodies (bnAbs) are advancing as a preventative or treatment option for HIV infection, but currently must be administered parenterally, typically as a large injection or infusion, an invasive method of delivery, especially for the pediatric population. Here, we present proof-of-concept data on a minimally invasive microneedle (MN) patch platform codelivering three bnAbs, PGT121, VRC07-523LS, and PGDM1400LS, from separate MN patch units or pixels. Each 1 cm² patch pixel, fabricated via solvent casting, contained similar to 2 mg of one bnAb. In ex vivo porcine skin tests, the MNs effectively penetrated the skin. When tested in vitro in PBS …

Sglt2 Inhibitors In Cardio-Oncology: A Systematic Review And Meta-Analysis, Luigi Spadafora, Marco Bernardi, Gianmarco Sarto, Beatrice Simeone, Erica Rocco, Salvatore Carbone, Laura Adelaide Dalla Vecchia, Roberto Franco Enrico Pedretti, Valentina Valentine, Rachele Di Mario, Maurizio Forte, Giacomo Frati, Antonio Abbate, Michele Golino, Francesco Versaci, Mariangela Peruzzi, Pierre Sabouret, Giuseppe Biondi Zoccai, Sebastiano Sciarretta

EVMS School of Health Professions Faculty Publications

Background

According to available evidence, sodium-glucose cotransporter-2 inhibitors (SGLT2i) may confer cardioprotection in patients with cancer undergoing chemotherapy.

Objectives

The objective of the study was to evaluate the impact of SGLT2i on all-cause mortality and heart failure (HF) outcomes in this population.

Methods

We searched PubMed, Cochrane CENTRAL, and Embase through August 2025 for studies of SGLT2i in adult patients with cancer. Random-effects models were used to pool effects for all-cause mortality and an HF composite (new-onset HF and/or HF hospitalization) in cancer patients >18 years. Meta-regression tested effect modification by beta-blockers, statins, angiotensin-converting enzyme inhibitors/angiotensin receptor blockers, age, and …

Turnover Time In Neuroangiography Suites: Comparison Between Cases Performed At An Ambulatory Neurosurgery Center Versus A Tertiary Hospital, Pui Man Rosalind Lai, Vinay Jaikumar, Erika L. Leasher, Varun Sridhar, Gustavo M. Cortez, Aimee C. Degaetano, Elad I. Levy

EVMS School of Health Professions Faculty Publications

Background:

Efficient turnover time (TOT) and adherence to first-start time (FST) in operating and angiography suites are critical for optimizing workflow, reducing costs, and maximizing patient satisfaction. Hospitals face challenges in maintaining these metrics due to operational constraints and unexpected emergent cases. Our goal was to compare TOT, FST, and total admission time for elective cases performed in angiography suites at an ambulatory neurosurgery center (ANSC) versus a tertiary hospital (TH).

Methods:

We conducted a retrospective analysis of data for patients who underwent elective diagnostic or interventional neuroendovascular procedures at the TH or ANSC from January 2024 through October 2024. …

Implementation And Clinical Utility Of Ultra-Low-Field Portable Magnetic Resonance Imaging For Postprocedural Neurological Evaluation In Ambulatory Neurosurgery: Illustrative Cases, Devan Patel, Vinay Jaikumar, Taysia P. T. Morioka, Laz Rifkin, Kenneth S. Jacoby, Jaims Lim, Anais Andrade, Aimee C. Degaetano, Pui Man Rosalind Lai, Elad I. Levy

EVMS School of Health Professions Faculty Publications

BACKGROUND

Elective endovascular neurosurgical procedures are increasingly performed in ambulatory neurosurgery centers, enabled by advances in catheter technology, safety of conscious sedation, and refined patient selection. Although complication rates are low, rapid evaluation of postprocedural neurological deficits remains critical. Conventional MRI is often impractical in outpatient or procedural settings, whereas ultra-low-field portable MRI (ULF-pMRI) systems (such as Swoop) allow bedside imaging with favorable diagnostic performance.

OBSERVATIONS

Two women in their 60s developed acute neurological deficits at an ambulatory neurosurgery center (ANSC) after diagnostic cerebral angiography in one case and elective internal carotid artery flow diversion in the other. In both …