Digital Commons Network^™

Tick Genome Assembled: New Opportunities For Research On Tick-Host-Pathogen Interactions, Jose De La Fuente, Robert M. Waterhouse, Daniel E. Sonenshine, R. Michael Roe, Jose M. Ribeiro, David B. Sattelle, Catherine A. Hill

Biological Sciences Faculty Publications

As tick-borne diseases are on the rise, an international effort resulted in the sequence and assembly of the first genome of a tick vector. This result promotes research on comparative, functional and evolutionary genomics and the study of tick-host-pathogen interactions to improve human, animal and ecosystem health on a global scale.

Geospatial Approaches To Support Pelagic Conservation Planning And Adaptive Management, L. M. Wedding, Sara M. Maxwell, D. Hyrenbach, D. C. Dunn, J. J. Roberts, D. Briscoe, E. Hines, P. N. Halpin

Biological Sciences Faculty Publications

Place-based management in the open ocean faces unique challenges in delineating boundaries around temporally and spatially dynamic systems that span broad geographic scales and multiple management jurisdictions, especially in the 'high seas'. Geospatial technologies are critical for the successful design of pelagic conservation areas, because they provide information on the spatially and temporally dynamic oceanographic features responsible for driving species distribution and abundance in the open ocean, the movements of protected species, and the spatial patterns of distribution of potential threats. Nevertheless, there are major challenges to implementing these geospatial approaches in the open ocean. This Theme Section seeks to …

The Sea Is Rising… But Not Onto The Policy Agenda: A Multiple Streams Approach To Understanding Sea Level Rise Policies, Juita-Elena (Wie) Yusuf, Katharine Neill, Burton St. John Iii, Ivan K. Ash, Kaitrin Mahar

School of Public Service Faculty Publications

There has been little policy effort to address sea level rise in coastal states in the US. It is important to examine, at the state level, how the multitude of different (and changing) actors with different preferences and perspectives contribute to such inertia. This study examines state-level legislative inaction with regards to sea level rise. Using Kingdon's multiple streams framework, we draw a picture of the policy landscape in Virginia as one where the problem of sea level rise is perceived as a low priority, with little consensus on achievable policy solutions, and is politically controversial. We find that policy …

Red List Of Marine Bony Fishes Of The Eastern Central Atlantic, Beth Polidoro, Gina Ralph, Kyle Strongin, Michael Harvey, Kent Carpenter, Titus Ayo Adeofe, Rachel Arnold, Paul Bannerman, Jean Noel Bibang Bi Nguema, Jack Buchanan, Khairdine Mohamed Abdallahi Camara, Youssouf Hawa Camara, Kadiatou Cissoko, Bruce B. Collette, Mia T. Comeros-Raynal, Godefroy De Bruyne, Madeleine Diouf, Roger Djiman, Mathieu Ducrocq, Ofer Gon, Antony S. Harold, Heather Harwell, Craig Hilton-Taylor, Andrew Hines, P. Alexander Hulley, Tomio Iwamoto, Steen Knudsen, Jean De Dieu Lewembe, Christi Linardich, Ken Lindeman, Ebou Mass Mbye, Jean Egard Mikolo, Vanda Monteiro, Jean Bernard Mougoussi, Thomas Munroe, Jean Hervé Mve Beh, Francis K.E. Nunoo, Caroline Pollock, Stuart Poss, Richmond Quartey, Barry Russell, Alphonse Sagna, Catherine Sayer, Aboubacar Sidibe, William Smith-Vaniz, Emilie Stump, Mor Sylla, Luis Tito De Morais, Jean-Christophe Vié, Akanbi Williams

Biological Sciences Faculty Publications

The Red List of marine bony fishes of the Eastern Central Atlantic (ECA) is a review of the conservation status of all native marine bony fishes in ECA according to the global Categories and Criteria of the IUCN Red List of Threatened Species. It identifies those species that are threatened with extinction at the global level and occur within the ECA region. This comprehensive assessment, which is the first of its kind in the ECA, aims to provide improved knowledge of species presence and extinction risk status for the purposes of guiding conservation actions and improved policies for these species …

Selective Mutation Accumulation: A Computational Model Of The Paternal Age Effect, Eoin C. Whelan, Alexander C. Nwala, Christopher Osgood, Stephan Olariu

Biological Sciences Faculty Publications

Motivation: As the mean age of parenthood grows, the effect of parental age on genetic disease and child health becomes ever more important. A number of autosomal dominant disorders show a dramatic paternal age effect due to selfish mutations: substitutions that grant spermatogonial stem cells (SSCs) a selective advantage in the testes of the father, but have a deleterious effect in offspring. In this paper we present a computational technique to model the SSC niche in order to examine the phenomenon and draw conclusions across different genes and disorders.

Results: We used a Markov chain to model the probabilities of …

Hsp90 Inhibition Suppresses Nf-Κb Transcriptional Activation Via Sirt-2 In Human Lung Microvascular Endothelial Cells, Gagan S. Thangjam, Charalampos Birmpas, Nektarios Barabutis, Betsy W. Gregory, Mary Ann Clemens, Joseph R. Newton, David Fulton, John D. Catravas

Bioelectrics Publications

The ability of anti-heat shock protein 90 (Hsp90) drugs to attenuate NF-κB-mediated transcription is the major basis for their anti-inflammatory properties. While the molecular mechanisms underlying this effect are not clear, they appear to be distinct in human endothelial cells. We now show for the first time that type 2 sirtuin (Sirt-2) histone deacetylase binds human NF-κB target gene promoter and prevents the recruitment of NF-κB proteins and subsequent assembly of RNA polymerase II complex in human lung microvascular endothelial cells. Hsp90 inhibitors stabilize the Sirt-2/promoter interaction and impose a “transcriptional block,” which is reversed by either inhibition or downregulation …

A Flexible Pet-Based Wearable Sensor For Arterial Pulse Waveform Measurement, Dan Wang, Dean Krusienski, Zhili Hao

Mechanical & Aerospace Engineering Faculty Publications

In light of the need of health monitoring, the paper presents a flexible polyethylene terephthalate (PET)-based wearable sensor for arterial pulse waveform measurement. The sensor encompasses a polydimethylsiloxane (PDMS) microstructure embedded with an electrolyte-enabled 5×1 transducer array, which spans 6mm and has a spatial resolution of 1.5mm. A pulse signal exerts a deflection on the microstructure and is recorded as a resistance change by a transducer at the site of the pulse. An untrained individual can easily align the sensor on a targeted artery with a negligible margin and then acquire the arterial pulse waveform continuously and non-invasively. This sensor …

Gender Differences In Vo2 And Hr Kinetics At The Onset Of Moderate And Heavy Exercise Intensity In Adolescents, Nicola Lai, Alessandro Martis, Alfredo Belfiori, Fatima Tolentino-Silva, Melita M. Nasca, James Strainic, Marco E. Cabrera

Electrical & Computer Engineering Faculty Publications

The majority of the studies on VO₂ kinetics in pediatric populations investigated gender differences in prepubertal children during submaximal intensity exercise, but studies are lacking in adolescents. The purpose of this study was to test the hypothesis that gender differences exist in the VO₂ and heart rate (HR) kinetic responses to moderate (M) and heavy (H) intensity exercise in adolescents. Twenty-one healthy African-American adolescents (9 males, 15.8 ± 1.1 year; 12 females, 15.7 ± 1 year) performed constant work load exercise on a cycle ergometer at M and H. The VO₂ kinetics of the male group was …

Stem In General Education: Does Mathematics Competence Influence Course Selection, Mary C. Enderson, John Ritz

STEMPS Faculty Publications

Many students enroll in college programs to prepare for their future careers. All are required to complete general studies courses. At one university, technology and STEM courses fulfill a part of the natural science and technology general education requirements. This study uses a survey design to explore why 332 students chose to enroll in a STEM technology course. Results found most enroll because their advisor suggests the course, it meets a general education major requirement, and the course is offered at a convenient time. Fewer enroll in the course because they would like to find out more about STEM fields, …

Comparing High School Students' And Adults' Perceptions Of Technology, Henry L. (Hal) Harrison Ii, Philip A. Reed

STEMPS Faculty Publications

This study compared high school student’s perceptions of technology and technological literacy to those perceptions of the general public. Additionally, individual student groups were compared statistically to determine significant differences between the groups. The ITEA/Gallup Poll instrument was used to survey high school student’s perceptions of technology in the study. The student population in question consisted of three subgroups: students enrolled in a standards-based technology education courses, students enrolled in a Project Lead the Way (PLTW) Principles of Engineering pre-engineering course, and students enrolled in a general education course (language arts, mathematics, or science). In addition, each student group’s perceptions …

Towards A Persuasive Design Pattern For A Gamified M-Learning Environment, Ferial Khaddage, Christoph Lattemann, Helen Crompton

Teaching & Learning Faculty Publications

New innovative technologies create opportunities for persuasive engagement. Persuasive technology is all about software, systems and applications designed to hook, reinforce, change and shape the attitudes of the learners without using coercion or deception. Persuasive design pattern if applied effectively and efficiently may influence learners’ attitudes towards the learning task, and may hook them to the specific learning activity offered via a gamified mobile application. In this paper the concept of persuasive design pattern in gamified m-learning platforms should be introduced to the scientific community. Persuasive design in a gamified mobile learning environment is described and persuasive set of design …

Contributors, W. Scott Howard

Reconstruction: Studies in Contemporary Culture

Information about contributors to articles in volume 16, issue 1 of Reconstruction.

Intracellular Ros Mediates Gas Plasma-Facilitated Cellular Transfection In 2d And 3d Cultures, Dehui Xu, Biqing Wang, Yujing Xu, Zeyu Chen, Qinjie Cui, Yanjie Yang, Hailan Chen, Michael G. Kong

Bioelectrics Publications

This study reports the potential of cold atmospheric plasma (CAP) as a versatile tool for delivering oligonucleotides into mammalian cells. Compared to lipofection and electroporation methods, plasma transfection showed a better uptake efficiency and less cell death in the transfection of oligonucleotides. We demonstrated that the level of extracellular aqueous reactive oxygen species (ROS) produced by gas plasma is correlated with the uptake efficiency and that this is achieved through an increase of intracellular ROS levels and the resulting increase in cell membrane permeability. This finding was supported by the use of ROS scavengers, which reduced CAP-based uptake efficiency. In …

Use Of Dynamic Visualizations For Engineering Technology, Industrial Technology, And Science Education Students: Implications On Ability To Correctly Create A Sectional View Sketch, Petros J. Katsioloudis, Daniel Dickerson, Vukica Jovanovic, Mildred V. Jones

Engineering Technology Faculty Publications

Spatial abilities, specifically visualization, play a significant role in the achievement in a wide array of professions including, but not limited to, engineering, technical, mathematical, and scientific professions. However, there is little correlation between the advantages of spatial ability as measured through the creation of a sectional-view sketch between engineering technology, industrial technology, and science education students. A causal-comparative study was selected as a means to perform the comparative analysis of spatial visualization ability. This study was done to determine the existence of statistically significant difference between engineering technology, industrial technology, and science education students’ ability to correctly create a …

Race, Ethnicity, Psychosocial Factors, And Telomere Length In A Multicenter Setting, Shannon M. Lynch, M. K. Peek, Nandita Mitra, Krithika Ravichandran, Charles Branas, Elaine Spangler, Wenting Zhou, Electra D. Paskett, Sarah Gehlert, Cecilia Degraffinreid, Timothy R. Rebbeck, Harold Riethman

School of Medical Diagnostics & Translational Sciences Publications

Background

Leukocyte telomere length(LTL) has been associated with age, self-reported race/ethnicity, gender, education, and psychosocial factors, including perceived stress, and depression. However, inconsistencies in associations of LTL with disease and other phenotypes exist across studies. Population characteristics, including race/ethnicity, laboratory methods, and statistical approaches in LTL have not been comprehensively studied and could explain inconsistent LTL associations.

Methods

LTL was measured using Southern Blot in 1510 participants from a multi-ethnic, multi-center study combining data from 3 centers with different population characteristics and laboratory processing methods. Main associations between LTL and psychosocial factors and LTL and race/ethnicity were evaluated and then …

Nanosecond Pulsed Platelet-Rich Plasma (Nsprp) Improves Mechanical And Electrial Cardiac Function Following Myocardial Reperfusion Injury, Barbara Y. Hargrave, Frency Varghese, Nektarios Barabutis, John Catravas, Christian Zemlin

School of Medical Diagnostics & Translational Sciences Publications

Ischemia and reperfusion (I/R) of the heart is associated with biochemical and ionic changes that result in cardiac contractile and electrical dysfunction. In rabbits, platelet-rich plasma activated using nanosecond pulsed electric fields (nsPRP) has been shown to improve left ventricular pumping. Here, we demonstrate that nsPRP causes a similar improvement in mouse left ventricular function. We also show that nsPRP injection recovers electrical activity even before reperfusion begins. To uncover the mechanism of nsPRP action, we studied whether the enhanced left ventricular function in nsPRP rabbit and mouse hearts was associated with increased expression of heat-shock proteins and altered mitochondrial …

Modeling Early Stage Bone Regeneration With Biomimetic Electrospun Fibrinogen Nanofibers And Adipose-Derived Mesenchymal Stem Cells, Michael P. Francis, Yas M. Moghaddam-White, Patrick C. Sachs, Matthew J. Beckman, Stephen M. Chen, Gary L. Bowlin, Lynne W. Elmore, Shawn E. Holt

School of Medical Diagnostics & Translational Sciences Publications

The key events of the earliest stages of bone regeneration have been described in vivo although not yet modeled in an in vitro environment, where mechanistic cell-matrix-growth factor interactions can be more effectively studied. Here, we explore an early-stage bone regeneration model where the ability of electrospun fibrinogen (Fg) nanofibers to regulate osteoblastogenesis between distinct mesenchymal stem cells populations is assessed. Electrospun scaffolds of Fg, polydioxanone (PDO), and a Fg:PDO blend were seeded with adipose-derived mesenchymal stem cells (ASCs) and grown for 7-21 days in osteogenic differentiation media or control growth media. Scaffolds were analyzed weekly for histologic and molecular …

Accessible Bioprinting: Adaptation Of A Low-Cost 3d-Printer For Precise Cell Placement And Stem Cell Differentiation, John A. Reid, Peter A. Mollica, Garett D. Johnson, Roy C. Ogle, Robert D. Bruno, Patrick C. Sachs

School of Medical Diagnostics & Translational Sciences Publications

The precision and repeatability offered by computer-aided design and computer-numerically controlled techniques in biofabrication processes is quickly becoming an industry standard. However, many hurdles still exist before these techniques can be used in research laboratories for cellular and molecular biology applications. Extrusion-based bioprinting systems have been characterized by high development costs, injector clogging, difficulty achieving small cell number deposits, decreased cell viability, and altered cell function post-printing. To circumvent the high-price barrier to entry of conventional bioprinters, we designed and 3D printed components for the adaptation of an inexpensive 'off-the-shelf' commercially available 3D printer. We also demonstrate via goal based …

Enhanced Osseous Integration Of Human Trabecular Allografts Following Surface Modification With Bioactive Lipids, Tiffany Wang, Jack Krieger, Cynthia Huang, Anusuya Das, Michael P. Francis, Roy Ogle, Edward Botchwey

School of Medical Diagnostics & Translational Sciences Publications

In this study, we used extracellular matrix (ECM) gels and human bone allograft as matrix vehicles to deliver the sphingolipid growth factor FTY720 to rodent models of tibial fracture and a critical-sized cranial defect. We show that FTY720 released from injectable ECM gels may accelerate callous formation and resolution and bone volume in a mouse tibial fracture model. We then show that FTY720 binds directly to human trabecular allograft bone and releases over 1 week in vitro. Rat critical-sized cranial defects treated with FTY720-coated grafts show increases in vascularization and bone deposition, with histological and micro-computed topography (microCT) evidence of …

Deep Models For Brain Em Image Segmentation: Novel Insights And Improved Performance, Ahmed Fakhry, Hanchuan Peng, Shuiwang Ji

Computer Science Faculty Publications

Motivation: Accurate segmentation of brain electron microscopy (EM) images is a critical step in dense circuit reconstruction. Although deep neural networks (DNNs) have been widely used in a number of applications in computer vision, most of these models that proved to be effective on image classification tasks cannot be applied directly to EM image segmentation, due to the different objectives of these tasks. As a result, it is desirable to develop an optimized architecture that uses the full power of DNNs and tailored specifically for EM image segmentation.

Results: In this work, we proposed a novel design of DNNs for …

A New Way To Estimate The Potential Unmet Need For Infertility Services Among Women In The United States, Arthur L. Greil, Kathleen S. Slauson-Blevins, Stacy Tiemeyer, Julia Mcquilan, Karina M. Shreffler

Sociology & Criminal Justice Faculty Publications

Background: Fewer than 50% of women who meet the medical/behavioral criteria for infertility receive medical services. Estimating the number of women who both meet the medical/behavioral criteria for infertility and who have pro-conception attitudes will allow for better estimates of the potential need and unmet need for infertility services in the United States.

Methods: The National Survey of Fertility Barriers was administered by telephone to a probability sample of 4,712 women in the United States. The sample for this analysis was 292 women who reported an experience of infertility within 3 years of the time of the interview. Infertile women …

Rcd+: Fast Loop Modeling Server, José R. López-Blanco, Alejandro J. Canosa-Valis, Yaohang Li, Pablo Chacón

Computer Science Faculty Publications

Modeling loops is a critical and challenging step in protein modeling and prediction. We have developed a quick online service (http://rcd.chaconlab.org) for ab initio loop modeling combining a coarse-grained conformational search with a full-atom refinement. Our original Random Coordinate Descent (RCD) loop closure algorithm has been greatly improved to enrich the sampling distribution towards near-native conformations. These improvements include a new workflow optimization, MPI-parallelization and fast backbone angle sampling based on neighbor-dependent Ramachandran probability distributions. The server starts by efficiently searching the vast conformational space from only the loop sequence information and the environment atomic coordinates. The generated closed loop …

Alcohol Expectancies Mediate The Relationship Between Age Of First Intoxication And Drinking Outcomes In College Binge Drinkers, Amy L. Stamates, Cathy Lau-Barraco, Ashley Linden-Carmichael

Psychology Faculty Publications

Background: While prior research has shown that age of first intoxication (AI) is associated with negative alcohol outcomes, limited research has examined factors accounting for this relationship. Alcohol expectancies, or beliefs about the effects of alcohol, may explain such associations as both positive and negative expectancies have been shown to be key predictors of drinking outcomes.

Objective: The present study examined expectancies as mediators between early AI and alcohol-related outcomes.

Method: Data collection occurred in 2012 and 2013. Participants were college students (N = 562, 65.8% women) who completed an online survey including measures of alcohol use history, alcohol expectancies, …

The Course And Interrelationship Of Maternal And Paternal Perinatal Depression, James F. Paulson, Sharnail D. Bazemore, Janice H. Goodman, Jenn A. Leiferman

Psychology Faculty Publications

The aims of the study were to describe course of depression in both mothers and fathers from the third trimester of pregnancy through 6 months postpartum and to examine the relationship between maternal and paternal depression. Hypotheses were as follows: (a) Depressive symptoms would be correlated between parents and (b) earlier depressive symptoms in one parent would predict later increases in depression in the other. Eighty cohabitating primiparous couples were recruited from prenatal OBGYN visits and community agencies and enrolled during pregnancy, between 28-week gestation and delivery. Participants completed measures of depression on four occasions: baseline and 1, 3, and …

Early Adverse Experiences And Health: The Transition To College, Kelly B. Filipkowski, Kristin E. Heron, Joshua M. Smyth

Psychology Faculty Publications

Objective: This study cross-sectionally and prospectively examined the impact of adversity experienced prior to college on the health and well-being of students adjusting to their first college semester. Methods: Two-hundred sixteen (216) first-year students completed measures of adverse life experiences, perceived stress, physical symptoms, and health-related behaviors during the first 2 weeks of college entry and again at the end of the first semester. Results: Reported adversity prior to college predicted greater perceived stress and physical symptoms at college entry and an increase in physical symptoms over the semester; perceived stress mediated the prospective changes. Early adversity …

Initiation And Retention In Couples Outpatient Treatment For Parents With Drug And Alcohol Use Disorders, Abbly L. Braitman, Michelle L. Kelley

Psychology Faculty Publications

The focus of the current study was to identity mental health, relationship factors, substance use related problems, and individual factors as predictors of couples-based substance abuse treatment initiation and attendance. Heterosexual couples with children that met study criteria were invited to attend 12 sessions of outpatient behavioral couples therapy. Men were more likely to initiate treatment if they had a higher income, had greater relationship satisfaction, were initiating treatment for alcohol use disorder only, were younger when they first suspected a problem, and had higher depression but lower hostility or phobic anxiety. Men attended more treatment sessions if they reported …

Acceptability Of Aggression Among Children Who Reside With Substance-Abusing Parents: The Influence Of Behavioral Dysregulation, Exposure To Neighborhood Violence, And Interparental Violence, Michelle L. Kelley, Abby L. Braitman, Robert J. Milletich, Brittany F. Hollis, Rachel E. Parsons, Tyler D. White, Cassie Patterson, Brianna Haislip, J. Matthew Henson

Psychology Faculty Publications

The present study examined how interparental violence, neighborhood violence, behavioral regulation during parental conflict, and age predicted beliefs about the acceptability of aggression and the acceptance of retaliation against an aggressive peer among youths. Participants were 110 families (mothers, fathers, and children) in which one or both parents met criteria for substance use disorder. Results of a bootstrapped multivariate regression model revealed higher exposure to neighborhood violence predicted greater acceptability of general aggression, whereas higher father-to-mother violence perpetration predicted lower acceptability of general aggression. Higher exposure to neighborhood violence, behavioral dysregulation during parental conflict, and older child age predicted greater …

Ultra-Compact Raman Spectrometer For Planetary Explorations, Derek Davis, James Hornef, John Lucas, Hani Elsayed-Ali, M. Nural Abedin

Electrical & Computer Engineering Faculty Publications

To develop a compact Raman spectroscopy system with features that will make it suitable for future space missions which require surface landing. Specifically, this system will be appropriate for any mission in which planetary surface samples need to be measured and analyzed.

Are We Missing Important Areas In Pelagic Marine Conservation? Redefining Conservation Hotspots In The Ocean, Dana K. Briscoe, Sara M. Maxwell, Raphael Kudela, Larry B. Crowder

Biological Sciences Faculty Publications

The protection of biodiversity is one of the most important goals in terrestrial and marine conservation. Marine conservation approaches have traditionally followed the example of terrestrial initiatives. However, patterns, processes, habitats, and threats differ greatly between the 2 systems - and even within the marine environment. As a result, there is still a lack of congruence as to how to best identify and prioritize conservation approaches moving from the static terrestrial and nearshore realm into a more fluid, 3-dimensional pelagic realm. To address this problem, we investigate how the conservation science literature has been used to inform and guide management …

A Novel Method Of Microsatellite Genotyping-By-Sequencing Using Individual Combinatorial Barcoding, Salla Vartia, Jose L. Villanueva-Cañas, John Finarelli, Edward D. Farrell, Patrick C. Collins, Graham M. Hughes, Jeanette E.L. Carlson, David T. Gauthier, Philip Mcginnity, Thomas F. Cross, Richard D. Fitzgerald, Luca Mirimin, Fiona Crispie, Paul D. Cotter, Jens Carlsson

Biological Sciences Faculty Publications

This study examines the potential of next-generation sequencing based 'genotyping-by-sequencing' (GBS) of microsatellite loci for rapid and cost-effective genotyping in large-scale population genetic studies. The recovery of individual genotypes from large sequence pools was achieved by PCR-incorporated combinatorial barcoding using universal primers. Three experimental conditions were employed to explore the possibility of using this approach with existing and novel multiplex marker panels and weighted amplicon mixture. The GBS approach was validated against microsatellite data generated by capillary electrophoresis. GBS allows access to the underlying nucleotide sequences that can reveal homoplasy, even in large datasets and facilitates cross laboratory transfer. GBS …