Digital Commons Network^™

Aberrant Epigenomic Modulation Of Glucocorticoid Receptor Gene (Nr3c1) In Early Life Stress And Major Depressive Disorder Correlation: Systematic Review And Quantitative Evidence Synthesis, Laurens Holmes Jr., Emily Shutman, Chinacherem Chinaka, Kerti Deepika, Lavisha Palaez, Kirk W. Dabney

Community & Environmental Health Faculty Publications

Early life stress (ELS) induced by psychological trauma, child maltreatment, maternal separation, and domestic violence predisposes to psycho-behavioral pathologies during adulthood, namely major depressive disorder (MDD), anxiety, and bipolar affective disorder. While environmental data are available in illustrating this association, data remain to be established on the epigenomic underpinning of the nexus between ELS and MDD predisposition. Specifically, despite the observed aberrant epigenomic modulation of the NR3C1, a glucocorticoid receptor gene, in early social adversity and social threats in animal and human models, reliable scientific data for intervention mapping in reducing social adversity and improving human health is required. We …

Implication Of Spiritual Network Support System In Epigenomic Modulation And Health Trajectory, Laurens Holmes Jr., Chinacherem Chinaka, Hikma Elmi, Kerti Deepika, Lavisha Palaez, Michael Enwere, Olumuyiwa T. Akinola, Kirk W. Dabnet

Community & Environmental Health Faculty Publications

With challenges in understanding the multifactorial etiologies of disease and individual treatment effect heterogeneities over the past four decades, much has been acquired on how physical, chemical and social environments a ffect human health, predisposing certain subpopulations to adverse health outcomes, especially the socio-environmentally disadvantaged (SED). Current translational data on gene and adverse environment interaction have revealed how adverse gene-environment interaction, termed aberrant epigenomic modulation, translates into impaired gene expression via messenger ribonucleic acid (mRNA) dysregulation, reflecting abnormal protein synthesis and hence dysfunctional cellular differentiation and maturation. The environmental influence on gene expression observed in most literature includes physical, chemical, …

Label-Free Microrna Optical Biosensors, Meimei Lai, Gymama Slaughter

Bioelectrics Publications

MicroRNAs (miRNAs) play crucial roles in regulating gene expression. Many studies show that miRNAs have been linked to almost all kinds of disease. In addition, miRNAs are well preserved in a variety of specimens, thereby making them ideal biomarkers for biosensing applications when compared to traditional protein biomarkers. Conventional biosensors for miRNA require fluorescent labeling, which is complicated, time-consuming, laborious, costly, and exhibits low sensitivity. The detection of miRNA remains a big challenge due to their intrinsic properties such as small sizes, low abundance, and high sequence similarity. A label-free biosensor can simplify the assay and enable the direct detection …

Seaflow Data V1, High-Resolution Abundance, Size And Biomass Of Small Phytoplankton In The North Pacific, François Ribalet, Chris Berthiaume, Annette Hynes, Jarred Swalwell, Michael Carlson, Sophie Clayton, Gwenn Hennon, Camille Poirier, Eric Shimabukuro, Angelicque White, E. Virginia Armhurst

OES Faculty Publications

SeaFlow is an underway flow cytometer that provides continuous shipboard observations of the abundance and optical properties of small phytoplankton (μm in equivalent spherical diameter, ESD). Here we present data sets consisting of SeaFlow-based cell abundance, forward light scatter, and pigment fluorescence of individual cells, as well as derived estimates of ESD and cellular carbon content of picophytoplankton, which includes the cyanobacteria Prochlorococcus, Synechococcus and small-sized Crocosphaera (μm ESD), and picophytoplankton and nanophytoplankton (2–5 μm ESD). Data were collected in surface waters (≈5 m depth) from 27 oceanographic cruises carried out in the Northeast Pacific Ocean between 2010 and 2018. …

Closing The Oxygen Mass Balance In Shallow Coastal Ecosystems, Matthew H. Long, Jennie E. Rheuban, Daniel C. Mccorkle, David J. Burdige, Richard C. Zimmerman

OES Faculty Publications

The oxygen concentration in marine ecosystems is influenced by production and consumption in the water column and fluxes across both the atmosphere-water and benthic-water boundaries. Each of these fluxes has the potential to be significant in shallow ecosystems due to high fluxes and low water volumes. This study evaluated the contributions of these three fluxes to the oxygen budget in two contrasting ecosystems, a Zostera marina (eelgrass) meadow in Virginia, U.S.A., and a coral reef in Bermuda. Benthic oxygen fluxes were evaluated by eddy covariance. Water column oxygen production and consumption were measured using an automated water incubation system. Atmosphere-water …

A New 30 Meter Resolution Global Shoreline Vector And Associated Global Islands Database For The Development Of Standardized Ecological Coastal Units, Roger Sayre, Suzanne Noble, Sharon Hamann, Rebecca Smith, Dawn Wright, Sean Breyer, Kevin Butler, Keith Van Graafeiland, Charlie Frye, Deniz Karagulle, Dabney Hopkins, Drew Stephens, Kevin Kelly, Zeenatul Basher, Devon Burton, Jill Cress, Karina Atkins, D. Paco Van Sistine, Beverly Friesen, Revecca Allee, Tom Allen, Peter Aniello, Irawan Asaad, Mark John Costello, Kathy Goodin, Peter Harris, Maria Kavanaugh, Helen Lillis, Elonora Manca, Frank Muller-Karger, Bjorn Nyberg, Rost Parsons, Jusrin Saarinen, Jack Steiner, Adam Reed

Political Science & Geography Faculty Publications

A new 30-m spatial resolution global shoreline vector (GSV) was developed from annual composites of 2014 Landsat satellite imagery. The semi-automated classification of the imagery was accomplished by manual selection of training points representing water and non-water classes along the entire global coastline. Polygon topology was applied to the GSV, resulting in a new characterisation of the number and size of global islands. Three size classes of islands were mapped: continental mainlands (5), islands greater than 1 km² (21,818), and islands smaller than 1 km² (318,868). The GSV represents the shore zone land and water interface boundary, and …

Multiple Cytosolic Dna Sensors Bind Plasmid Dna After Transfection, Nina Semenova, Masa Bosnjak, Katarina Znidar, Maja Cemazar, Loree Heller

Bioelectrics Publications

Mammalian cells express a variety of nucleic acid sensors as one of the first lines of defense against infection. Despite extensive progress in the study of sensor signaling pathways during the last decade, the detailed mechanisms remain unclear. In our previous studies, we reported increased type I interferon expression and the upregulation of several proposed cytosolic DNA sensors after transfection of several tumor cell types with plasmid DNA (pDNA). In the present study, we sought to reveal the early events in the cytosolic sensing of this nucleic acid in a myoblast cell line. We demonstrated that DNA-dependent activator of interferon …

The Flow Of Family Transitions Of Australian Families, Shana Pribesh, Matthew Usevitch, Elizabeth Koch Sigler, Kaijsa Angerhofer Heninger, Yuanyuan Yue, Mikaela J. Dufur, Jonathan A. Jarvis

Educational Leadership & Workforce Development Faculty Publications

Family structure disruption has been linked to negative child educational and health outcomes (Perales et al. 2016). Australia has relatively stable families, but income disparities between Australians are widening, and single-parent families make up a large proportion of families living in poverty. Cohabitation is also common in Australia with approximately three-quarters of marriages preceded by cohabitation. If substantial family structure churning affects Australian children this may expose a need for special policy interventions aimed at family creation and dissolution to ameliorate the negative effects of such stressful experiences. To highlight family structures and transitions Australian children experience, we use Sankey …

How Oxygen Availability Affects The Antimicrobial Efficacy Of Host Defense Peptides: Lessons Learned From Studying The Copper-Binding Peptides Piscidins 1 And 3, Adenrele Oludiran, David S. Courson, Malia D. Stuart, Anwar R. Radwan, John C. Putsma, Myriam L. Cotten, Erin B. Purcell

Chemistry & Biochemistry Faculty Publications

The development of new therapeutic options against Clostridioides difficile (C. difficile) infection is a critical public health concern, as the causative bacterium is highly resistant to multiple classes of antibiotics. Antimicrobial host-defense peptides (HDPs) are highly effective at simultaneously modulating the immune system function and directly killing bacteria through membrane disruption and oxidative damage. The copper-binding HDPs piscidin 1 and piscidin 3 have previously shown potent antimicrobial activity against a number of Gram-negative and Gram-positive bacterial species but have never been investigated in an anaerobic environment. Synergy between piscidins and metal ions increases bacterial killing aerobically. Here, we …

Dynamics Of Trophoblast Differentiation In Peri-Implantation–Stage Human Embryos, Rachel C. West, Hao Ming, Deirdre M. Logsdon, Jiangwen Sun, Sandeep K. Rajput, Rebecca A. Kile, William B. Schoolcraft, R. Michael Roberts, Rebecca L. Krisher, Zongliang Jiang, Ye Yuan

Computer Science Faculty Publications

Single-cell RNA sequencing of cells from cultured human blastocysts has enabled us to define the transcriptomic landscape of placental trophoblast (TB) that surrounds the epiblast and associated embryonic tissues during the enigmatic day 8 (D8) to D12 peri-implantation period before the villous placenta forms. We analyzed the transcriptomes of 3 early placental cell types, cytoTB (CTB), syncytioTB (STB), and migratoryTB (MTB), picked manually from cultured embryos dissociated with trypsin and were able to follow sublineages that emerged from proliferating CTB at the periphery of the conceptus. A unique form of CTB with some features of STB was detectable at D8, …

Evaluating Resilience: Insights From Florida, Hannah Torres

October 11, 2019: Measuring and Evaluating Resilience

No abstract provided.

Monarch Science Observer, Volume 1, College Of Sciences, Old Dominion University

College of Sciences Newsletter

Fall 2019, issue of Monarch Science Observer, ODU Colleges of Sciences Newsletter.

Circulation, Volume 25, No. 1, Center For Coastal Physical Oceanography, Old Dominion University, Tal Ezer

CCPO Circulation

Fall 2019 issue of CCPO Circulation featuring the article, "The International Reach of CCPO in Ocean Modeling and Education: Reflections from a Recent Trip to China," by Tal Ezer.

Stream Assessment On The Impact Of Agricultural Activity In The Dry River, Va, Heston Anderson, Nate Bickford

Virginia Journal of Science

Stream bioassessments using macroinvertebrate population dynamics is a technique that determines water quality in natural aquatic environments based on the taxa found at the site.  The aim of this study is to determine if agricultural activity in Rockingham County, VA has an impact on water quality in Dry River.  Stream quality was evaluated by sampling and identifying macroinvertebrate taxa at various sites above and below disturbances.  Each macroinvertebrate was ranked from 1-10 based on pollution tolerance or intolerance using the Biological Monitoring Working Party Index.  The results in this study indicate that agricultural activity does impact the water quality in …

Mercury Concentrations In Bat Guano From Caves And Bat Houses In Florida And Georgia, Amy E. Edwards, Jenise L. Swall, Charles H. Jagoe

Virginia Journal of Science

Previous studies have indicated that several pollutants are bioaccumulating in insectivorous bats, including the heavy metal mercury. This has resulted in an increased presence of mercury in bat waste (guano). In this study, we collected bat guano from ten caves in Florida and Georgia and two bat houses in Florida and analyzed the samples for mercury concentrations (ppm). Since the predominant bat species using caves (Myotis austroriparius) versus bat houses (Tadarida braziliensis) were different, the objective of this study was to make statistical comparisons of the mercury concentrations among caves, between caves and bat houses, and between …

Paper-Based Flexible Electrode Using Chemically-Modified Graphene And Functionalized Multiwalled Carbon Nanotube Composites For Electrophysiological Signal Sensing, Md Faruk Hossain, Jae Sang Heo, John Nelson, Insoo Kim

Bioelectrics Publications

Flexible paper-based physiological sensor electrodes were developed using chemically-modified graphene (CG) and carboxylic-functionalized multiwalled carbon nanotube composites (f@MWCNTs). A solvothermal process with additional treatment was conducted to synthesize CG and f@MWCNTs to make CG-f@MWCNT composites. The composite was sonicated in an appropriate solvent to make a uniform suspension, and then it was drop cast on a nylon membrane in a vacuum filter. A number of batches (0%~35% f@MWCNTs) were prepared to investigate the performance of the physical characteristics. The 25% f@MWCNT-loaded composite showed the best adhesion on the paper substrate. The surface topography and chemical bonding of the proposed CG-f@MWCNT …

Modeling Social Learning: An Agent-Based Approach, Erika G. Ardiles Cruz

Computational Modeling & Simulation Engineering Theses & Dissertations

Learning is the process of acquiring or modifying knowledge, behavior, or skills. The ability to learn is inherent to humans, animals, and plants, and even machines are provided with algorithms that could mimic in a restricted way the processes of learning. Humans learn from the time they are born until they die because of a continuous process of interaction between them and their environment. Behavioral Psychology Theories and Social Learning Theories study behavior learned from the environment and social interactions through stimulus-response. Some computer approaches to modeling human behavior attempted to represent the learning and decision-making processes using agent-based models. …

Demonstration Of Visible And Near Infrared Raman Spectrometers And Improved Matched Filter Model For Analysis Of Combined Raman Signals, Alexander Matthew Atkinson

Electrical & Computer Engineering Theses & Dissertations

Raman spectroscopy is a powerful analysis technique that has found applications in fields such as analytical chemistry, planetary sciences, and medical diagnostics. Recent studies have shown that analysis of Raman spectral profiles can be greatly assisted by use of computational models with achievements including high accuracy pure sample classification with imbalanced data sets and detection of ideal sample deviations for pharmaceutical quality control. The adoption of automated methods is a necessary step in streamlining the analysis process as Raman hardware becomes more advanced. Due to limits in the architectures of current machine learning based Raman classification models, transfer from pure …

The Effect Of Standardised Learning Diaries On Self-Regulated Learning, Calibration Accuracy And Academic Achievement, Avanelle Joseph-Edwards

STEMPS Theses & Dissertations

The online learning environment is a dynamic yet complex learning modality. Students are physically separated from their peers, they grapple with feelings of isolation, and they may be unable to self-regulate their learning. Studies have shown that self-regulation is related to academic achievement and student metacognitive monitoring in online settings. The present study investigated the effects of a standardized diaries on students’ self-regulatory behaviors, calibration accuracy and academic achievement within an online learning environment. Using this self-monitoring and evaluation tool, forty online graduate students enrolled in a research methods course at a southeastern university in the United States participated in …

Global Conservation Status And Threat Patterns Of The World’S Most Prominent Forage Fishes (Teleostei, Clupeiformes), Tiffany L. Birge

Biological Sciences Theses & Dissertations

Conserving biodiversity is one of the greatest ethical responsibilities and challenges humans face. Understanding the conservation status of taxonomic groups provides a systematic way to prioritize efforts to combat biodiversity loss. The 405 species within the order Clupeiformes are the herrings, shads, sardines, anchovies, menhadens and relatives that include many of the most important marine forage fishes. These small, schooling fishes are economically, ecologically and culturally significant globally. Despite their contribution to global fisheries and our increasing reliance on these fishes for food and industrial commodities, they are generally poorly known with limited information regarding basic biology and population trends. …

Numerical Modeling And Field Investigation Of Nearshore Nonlinear Wave Propagation, Elham Sharifineyestani

Civil & Environmental Engineering Theses & Dissertations

First, a phase-resolving frequency-domain wave model that solves nonlinear wave-wave interactions is improved to account for wave dissipation and modulations over viscoelastic mud layer. Model results show satisfactory agreement with laboratory measurements. The model is then used to investigate the combined effect of mud viscoelasticity and nonlinear wave-wave interactions on surface wave evolution using cnoidal and random wave simulations. In general, qualitative measures such as shape of cnoidal waves or pattern of variation in H_rms of random waves are dictated by direct mud-induced damping which, due to resonance effects, has a substantially different structure over viscoelastic mud compared to …

A Comparison Of Multi-Attribute Utility Theory, The Analytic Hierarchy Process, The Analytic Network Process, And New Hybrid Approaches For A Case Study Involving Radon, Jesse Ray Toepfer

Engineering Management & Systems Engineering Theses & Dissertations

This dissertation focuses on the use of three new combinational hybrid approaches to solve a rational decision problem. Even though Multi-Attribute Utility Theory (MAUT) and the Analytic Hierarchy Process (AHP), and the Analytic Network Process (ANP), are all long established theories, their practical applications continue to grow and generate new knowledge. As a case study approach, there exists a knowledge gap concerning the use of these MCDM methods in the area of environmental remediation, and more especially, in situations that involve radioactive materials. From this, and as borne out by the literature review discussed herein, a problem statement is thus …

Electrohydrodynamic Simulations Of The Deformation Of Liquid-Filled Capsules, Pai Song

Mathematics & Statistics Theses & Dissertations

A comprehensive two- and three-dimensional framework for the electrohydrodynamic simulation of deformable capsules is provided. The role of a direct current (DC) electric field on the deformation and orientation of a liquid-filled capsule is thoroughly considered numerically. This framework is based on lattice Boltzmann method for the fluid, finite element method for the membrane structure of the capsule, fast immersed interface method for the electric field and immersed boundary method being used to consider the fluid-structure-electric interaction. Under the effect of electric field, two different types of equilibrium states, prolate or oblate are obtained. The numerical algorithm is also applied …

Molecular Systematics Of Isoëtes (Isoëtaceae) In Eastern North America, Peter William Schafran

Biological Sciences Theses & Dissertations

Isoëtes (Isoëtaceae, Isoetales, Lycopodiophyta) is a cosmopolitan genus of aquatic lycophytes, occurring on every continent except Antarctica. Of approximately 200 total taxa, about half are in a clade of species mostly occurring in North and South America. Eastern North America accounts for 22% of global taxonomic diversity, containing 32 fertile taxa and 16 named hybrids. This taxonomic diversity is built upon relatively little morphological difference, and even combined with phylogenetic analysis using several nuclear and chloroplast DNA markers, no well-resolved systematic treatment within this clade exists.

This study aims to clarify the relationships between all species and subspecies of Isoëtes …

Circum-Arctic Mineralogy & Pan-Arctic Chronostratigraphy Of Late Pleistocene Sediments: Developing A Comprehensive Age Model For The Western Arctic Ocean Using Unique Ice-Rafted Signals, Wesley Blake Myers

OES Theses and Dissertations

To improve understanding of geographic mineral distribution from the circum-Arctic Ocean, samples from the Arctic periphery were collected and analyzed for (semi-) quantitative mineral composition. Most samples were collected from the North American region of the Arctic Ocean, a region which has had limited mineral investigation. In addition, more than 1000 published clay mineral data points were gathered to provide the most comprehensive clay mineral distribution map to date. The identification of a smectite source within the Canadian Arctic may reduce the usefulness of this mineral as a unique provenance signal for the Eurasian region. Smectite speciation may be useful …

The Weight Of Words: A Content Analysis Of Rhetoric In Online News Articles Reporting On Sex Crimes, Julie Snell

Sociology & Criminal Justice Theses & Dissertations

The purpose of this study is to examine the rhetoric that is used in online news articles about sexual assault to see if discriminatory language is prevalent when reporting on different races. A content analysis of 100 online news articles was conducted to answer two research questions: 1) In what ways, if any, does the media use discriminatory rhetoric in online news articles when reporting on sexual assault offenders who are, or appear to be, racial minorities? 2) In what ways, if any, does the media use discriminatory rhetoric in online news articles when reporting on sexual assault victims who …

Pacts 1.0: A Crowdsourced Reporting Standard For Paleoclimate Data, D. Khider, J. Emile-Geay, N. P. Mckay, Y. Gil, D. Garijo, V. Ratnakar, M. Alonso-Garcia, S. Bertrand, O. Bothe, P. Brewer, A. Bunn, M. Chevalier, L. Comas-Bru, J. Hertzberg, Y. Zhou

OES Faculty Publications

The progress of science is tied to the standardization of measurements, instruments, and data. This is especially true in the Big Data age, where analyzing large data volumes critically hinges on the data being standardized. Accordingly, the lack of community-sanctioned data standards in paleoclimatology has largely precluded the benefits of Big Data advances in the field. Building upon recent efforts to standardize the format and terminology of paleoclimate data, this article describes the Paleoclimate Community reporTing Standard (PaCTS), a crowdsourced reporting standard for such data. PaCTS captures which information should be included when reporting paleoclimate data, with the goal of …

The Diversity And Distribution Of Spiders (Arachnida: Araneae) Along An Outdoor – Indoor Habitat Gradient: Preliminary Findings From Piedmont Virginia, William Kish, Sujan Henkanaththegedara

Virginia Journal of Science

Although the United States supports a considerable diversity of spiders, some aspects of spider habitat use and niche specialization are poorly documented. Specifically, little attention has been given to explore how urban development affects the diversity and abundance of arthropods. We sampled spiders along an outdoor – indoor habitat gradient at Longwood University to understand the impact of urbanization on species diversity and abundance. We found 50 taxa of spiders belonging to 43 genera and 16 families. Overall, the most abundant spider family across three sampling sites was Araneidae (orb-weavers; 18.2%) followed by Lycosidae (wolf spiders; 14.8%), Salticidae (jumping spiders; …

Acute Systemic Inflammatory Response To Lipopolysaccharide Stimulation In Pigs Divergently Selected For Residual Feed Intake, Haibo Liu, Kristina M. Feye, Yet T. Nguyen, Anoosh Rakhshandeh, Crystal L. Loving, Jack C. M. Sekkers, Nicholas K. Gabler, Christopher K. Tuggle

Mathematics & Statistics Faculty Publications

Background: It is unclear whether improving feed efficiency by selection for low residual feed intake (RFI) compromises pigs’ immunocompetence. Here, we aimed at investigating whether pig lines divergently selected for RFI had different inflammatory responses to lipopolysaccharide (LPS) exposure, regarding to clinical presentations and transcriptomic changes in peripheral blood cells.

Results: LPS injection induced acute systemic inflammation in both the low-RFI and high-RFI line (n = 8 per line). At 4 h post injection (hpi), the low-RFI line had a significantly lower (p= 0.0075) mean rectal temperature compared to the high-RFI line. However, no significant differences in complete blood count …

A Delphi Study: Retention Of Women In Leadership Positions In Stem Disciplines, Kimberly T. Luthi

Educational Leadership & Workforce Development Theses & Dissertations

This Delphi study explores barriers and support systems that impact women’s professional advancement in STEM disciplines. There were 20 expert panelists who committed to participate in the study and 15 panelists completed the four rounds of the study after attrition. The panelists were selected based on specific criteria including educational background, diversity within STEM disciplines, experience as a former or current female administrator who served at two-year degree offering institutions, leadership and membership within women’s advocacy organizations in STEM and related workforce education fields, and depth of knowledge and understanding of the research questions. Through the four rounds of the …