Digital Commons Network^™

Blue Carbon Science, Management And Policy Across A Tropical Urban Landscape, Daniel A. Friess, Yasmine M. Gatt, Tze Kwan Fung, Jahson B. Alemu I, Natasha Bhatia, Rebecca Case, Siew Chin Chua, Danwei Huang, Valerie Kwan, Kiah Eng Lim, Yudhishthra Nathan, Yan Xiang Ow, Daniel Saavedra-Hortua, Taylor M. Sloey, Erik S. Yando, Hassan Ibrahim, Lian Pin Koh, Jun Yu Puah, Serena Lay-Ming Teo, Karenne Tun, Lynn Wei Wong, Siti Maryam Yaakub

Biological Sciences Faculty Publications

The ability of vegetated coastal ecosystems to sequester high rates of “blue” carbon over millennial time scales has attracted the interest of national and international policy makers as a tool for climate change mitigation. Whereas focus on blue carbon conservation has been mostly on threatened rural seascapes, there is scope to consider blue carbon dynamics along highly fragmented and developed urban coastlines. The tropical city state of Singapore is used as a case study of urban blue carbon knowledge generation, how blue carbon changes over time with urban development, and how such knowledge can be integrated into urban planning alongside …

Estimating The Distribution Of Oryzomys Palustris, A Potential Key Host In Expanding Rickettsial Tick-Borne Disease Risk, Catherine A. Lippi, Samuel Canfield, Christina Espada, Holly D. Gaff, Sadie J. Ryan

Biological Sciences Faculty Publications

Increasingly, geographic approaches to assessing the risk of tick-borne diseases are being used to inform public health decision-making and surveillance efforts. The distributions of key tick species of medical importance are often modeled as a function of environmental factors, using niche modeling approaches to capture habitat suitability. However, this is often disconnected from the potential distribution of key host species, which may play an important role in the actual transmission cycle and risk potential in expanding tick-borne disease risk. Using species distribution modeling, we explore the potential geographic range of Oryzomys palustris, the marsh rice rat, which has been …

The Coral Bleaching Automated Stress System (Cbass): A Low-Cost, Portable System For Standardized Empirical Assessments Of Coral Thermal Limits, Nicholas R. Evensen, Katherine E. Parker, Thomas A. Oliver, Stephen R. Palumbi, Cheryl A. Logan, James S. Ryan, Courtney N. Klepac, Gabriela Perna, Mark E. Warner, Christian R. Voolstra, Daniel J. Barshis

Biological Sciences Faculty Publications

Ocean warming is increasingly affecting marine ecosystems across the globe. Reef-building corals are particularly affected by warming, with mass bleaching events increasing in frequency and leading to widespread coral mortality. Yet, some corals can resist or recover from bleaching better than others. Such variability in thermal resilience could be critical to reef persistence; however, the scientific community lacks standardized diagnostic approaches to rapidly and comparatively assess coral thermal vulnerability prior to bleaching events. We present the Coral Bleaching Automated Stress System (CBASS) as a low-cost, open-source, field-portable experimental system for rapid empirical assessment of coral thermal thresholds using standardized temperature …

Variation In Establishment Success For American Mistletoe [Phoradendron Leucarpum (Raf.) Reveal & M.C. Johnst. (Viscaceae)] Appears Most Likely To Predict Its Distribution In Virginia And North Carolina, United States, Nicholas P. Flanders, Christopher P. Randle, Eric L. Walters, Lytton J. Musselman

Biological Sciences Faculty Publications

Dispersal limitation and variation in habitat suitability may determine an association of American mistletoe [Phoradendron leucarpum (Raf.) Reveal & M.C. Johnst. (Viscaceae)] with forested wetlands in Virginia and North Carolina, United States. Here, we first tested the alternative hypothesis that variation in host availability drives this habitat relationship. We used a generalized linear model to show a positive effect of forested wetland habitat on American mistletoe occurrence after accounting for both variation in host availability and differences among regions in host use. We then used seed sowing experiments to quantify how light availability and flood regime determine the viability …

Adjusting For Gene-Specific Covariates To Improve Rna-Seq Analysis, Hyeongseon Jeon, Kyu-Sang Lim, Yet Nguyen, Dan Nettleton

Mathematics & Statistics Faculty Publications

Summary

This paper suggests a novel positive false discovery rate (pFDR) controlling method for testing gene-specific hypotheses using a gene-specific covariate variable, such as gene length. We suppose the null probability depends on the covariate variable. In this context, we propose a rejection rule that accounts for heterogeneity among tests by employing two distinct types of null probabilities. We establish a pFDR estimator for a given rejection rule by following Storey's q-value framework. A condition on a type 1 error posterior probability is provided that equivalently characterizes our rejection rule. We also present a suitable procedure for selecting a tuning …

Preservice Secondary Science Teachers' Reflections In Using Modeling & Simulation Applications As Instructional Tools For Learning, Mary C. Enderson, Elizabeth Langran (Ed.), Paula Christensen (Ed.), Jarrod Sanson (Ed.)

Teaching & Learning Faculty Publications

Preparing preservice teachers to construct learning environments integrating technological tools is a challenge that higher education continues to tackle. One way to address this challenge is to have preservice teachers integrate modeling & simulation (M&S) tools while teaching, reflect on the experience, and attempt to identify ways to make improvements in instructional practices. This case study of five preservice secondary science teachers, enrolled in a course along with student teaching, was designed to study M&S tools integrated into instruction and how preservice teachers thought about the experience. Participants taught at least one lesson integrating M&S tools where they were to …

Enhancing The Conformational Stability Of The Cl-Par-4 Tumor Suppressor Via Site-Directed Mutagenesis, Samjhana Pandey, Krishna K. Raut, Antoine Baudin, Lamya Djemri, David S. Libich, Komala Ponniah, Steven M. Pascal

Chemistry & Biochemistry Faculty Publications

Intrinsically disordered proteins play important roles in cell signaling, and dysregulation of these proteins is associated with several diseases. Prostate apoptosis response-4 (Par-4), an approximately 40 kilodalton proapoptotic tumor suppressor, is a predominantly intrinsically disordered protein whose downregulation has been observed in various cancers. The caspase-cleaved fragment of Par-4 (cl-Par-4) is active and plays a role in tumor suppression by inhibiting cell survival pathways. Here, we employed site-directed mutagenesis to create a cl-Par-4 point mutant (D313K). The expressed and purified D313K protein was characterized using biophysical techniques, and the results were compared to that of the wild-type (WT). We have …

Enrollment Management Strategies At Rural Community Colleges Resulting From The Pandemic, Robin C. Daniel, Mitchell R. Williams, Kim E. Bullington

Educational Leadership & Workforce Development Faculty Publications

Higher education institutions around the world were impacted by the COVID-19 pandemic that began in early 2020. Because U.S. community colleges focus on two-year degrees and workforce development, they were affected differently than their four-year counterparts. This study examined how academic administrators at different rural community colleges in Virginia, United States, perceived enrollment management practices that were implemented or changed due to the pandemic. This sequential explanatory study's first phase was quantitative and measured mid- to senior-level administrators' views of how the pandemic affected their colleges' enrollment management practices. The second qualitative phase resulted in five themes surrounding COVID-related enrollment …

Expanding Our Grasp Of Two-Component Signaling In Clostridioides Difficile, Orlando Berumen Alvarez, Erin B. Purcell

Chemistry & Biochemistry Faculty Publications

The intestinal pathogen Clostridioides difficile encodes roughly 50 TCS, but very few have been characterized in terms of their activating signals or their regulatory roles. A. G. Pannullo, B. R. Zbylicki, and C. D. Ellermeier (J Bacteriol 205:e00164-23, 2023, https://doi.org/10.1128/jb.00164-23) have identified both for the novel C. difficile TCD DraRS. DraRS responds to antibiotics that target lipid-II molecules in the bacterial cell envelope, and regulates the production of a novel glycolipid necessary for bacitracin and daptomycin resistance in C. difficile.

Pursuing Professional Learning By Using Social Media: How Do Instructional Designers Apply Self-Regulated Learning?, Pauline Salim Muljana, Tian Luo

STEMPS Faculty Publications

The instructional design and technology field are dynamic, requiring instructional designers to stay abreast through timely professional learning. Social media offers characteristics to collapse the time, geographical, and financial limitations of informal professional learning, but challenges exist. Continuous professional learning requires proactive actions, wherein self-regulated learning (SRL) plays an important role. How-ever, not all professionals know the effective strategies to promote SRL skills. This study examines instructional designers' (N = 17) experiences of professional learning on social media through an SRL lens. Data collected through semi-structured inter-views were analyzed using thematic analysis. Findings include SRL strategies con-ducted by instructional designers …

Universal Design For Learning Implementation In Higher Education: Survey Of Faculty And Instructional Designers, Breanne A. Kirsch, Tian Luo

STEMPS Faculty Publications

Universal design for learning (UDL) is an inclusive design framework. Faculty and instructional designers were surveyed to investigate UDL implementation in higher education. This survey was based on one used by Westine et al. (2019). Results about how faculty and instructional designers learn about UDL, how instructional designers train and support faculty in UDL, and why and how participants choose to employ UDL are shared. The results from this study have provided new findings in relation to faculty and instructional designer UDL implementation efforts in higher education that have implications for training and supporting faculty with UDL application efforts.

Improving Reliability In Assessing Integrative Learning Using Rubrics: Does Group Norming Help?, Lanah Stafford, Erin Cousins, Linda Bol, Megan Mize

STEMPS Faculty Publications

Integrative learning is an important outcome for graduates of higher education. Therefore, it should be well-defined and assessed reliably. The American Association of Colleges & Universities has developed a rubric to define and assess integrative learning, but it has low reliability. This pilot study examines whether this rubric's reliability can be improved by training users on how to use the rubric in a group setting rather than individually. Twelve faculty were trained to score undergraduate ePortfolios using the Integrative and Applied Learning VALUE Rubric. Half of the faculty were trained in an individual setting and half in a group setting …

Application Of Mixture Models For Doubly Inflated Count Data, Monika Arora, N. Rao Chaganty

Mathematics & Statistics Faculty Publications

In health and social science and other fields where count data analysis is important, zero-inflated models have been employed when the frequency of zero count is high (inflated). Due to multiple reasons, there are scenarios in which an additional count value of k > 0 occurs with high frequency. The zero- and k-inflated Poisson distribution model (ZkIP) is more appropriate for such situations. The ZkIP model is a mixture distribution with three components: degenerate distributions at 0 and k count and a Poisson distribution. In this article, we propose an alternative and computationally fast expectation–maximization (EM) algorithm to obtain the parameter …

Biocybersecurity And Deterrence: Hypothetical Rwandan Considerations, Issah Samori, Gbadebo Odularu, Lucas Potter, Xavier-Lewis Palmer

Community & Environmental Health Faculty Publications

Digitalization and sustainability are popular words within modern disciplines as practitioners each look toward the future of their respective fields. Specifically for the African continent, which is making great strides in developmental targets, those two terms are central to core aspects of policy initiatives that may foster cooperation across its varied lands and nations. One of the underlying challenges that confront Africa is a lack of strong regional integration across socioeconomic and political programs; there is value in African regions having more regional connectedness. We assess the rate of regional integration and development in Africa and discuss how to alleviate …

Advancing Estuarine Shoreline Change Analysis Using Small Uncrewed Autonomous Systems, Thomas R. Allen, Devon Eulie, Mariko Polk, George Mcleod, Robert Stuart, Alexandra Garnand, A. J. Manning (Editor)

Political Science & Geography Faculty Publications

Estuarine shorelines face the threats of accelerating sea-level rise, recurrent storms, and disruptions of natural sediment and ecological adjustments owing to historic human interventions. The growing availability and technical capability of uncrewed systems (UxS), including remote or autonomous aerial and surface vessels, provide new opportunities to study and understand estuarine shoreline changes. This chapter assesses the state of the technology, interdisciplinary science and engineering literature, and presents case studies from the Chesapeake Bay, Virginia, and coastal North Carolina, USA, that demonstrate new insights into coastal geomorphic processes and applications to managing complex and dynamic estuarine shorelines. These technologies enhance the …

Racialization And International Security, Richard W. Maass

Political Science & Geography Faculty Publications

Racialization—the processes that infuse social and political phenomena with racial identities and implications—is an assertion of power, a claim of purportedly inherent differences that has saturated modern diplomacy, order, and violence. Despite the field's consistent interest in power, international security studies in the United States largely omitted racial dynamics from decades of debates about international conflict and cooperation, nuclear proliferation, power transitions, unipolarity, civil wars, terrorism, international order, grand strategy, and other subjects. A new framework lays conceptual bedrock, links relevant literatures to major research agendas in international security, cultivates interdisciplinary dialogues, and charts promising paths to consider how overt …

Speculative Futures On Chatgpt And Generative Artificial Intelligence (Ai): A Collective Reflection From The Educational Landscape, Aras Bozkurt, Junhong Xiao, Sarah Lambert, Angelica Pazurek, Helen Crompton, Suzan Koseoglu, Robert Farrow, Melissa Bond, Chrissi Nerantzi, Sarah Honeychurch, Maha Bali, Jon Dron, Kamran Mir, Bonnie Stewart, Eamon Costello, Jon Mason, Christian M. Stracke, Enilda Romero-Hall, Apostolos Koutropoulos, Cathy Mae Toquero, Lenandlar Singh, Ahmed Tlili, Kyungmee Lee, Mark Nichols, Ebba Ossiannilsson, Mark Brown, Valerie Irvine, Juliana Elisa Raffaghelli, Gema Santos-Hermosa, Orna Farrell, Taskeen Adam, Ying Li Thong, Sunagul Sani-Bozkurt, Ramesh C. Sharma, Stefan Hrastinski, Petar Jandrić

Teaching & Learning Faculty Publications

While ChatGPT has recently become very popular, AI has a long history and philosophy. This paper intends to explore the promises and pitfalls of the Generative Pre-trained Transformer (GPT) AI and potentially future technologies by adopting a speculative methodology. Speculative future narratives with a specific focus on educational contexts are provided in an attempt to identify emerging themes and discuss their implications for education in the 21st century. Affordances of (using) AI in Education (AIEd) and possible adverse effects are identified and discussed which emerge from the narratives. It is argued that now is the best of times to define …

Identification Of Proteins Involved In Cell Membrane Permeabilization By Nanosecond Electric Pulses (Nsep), Giedre Silkuniene, Uma Mangalanathan, Alessandra Rossi, Peter A. Mollica, Andrei G. Pakhomov, Olga N. Pakhomova

Bioelectrics Publications

The study was aimed at identifying endogenous proteins which assist or impede the permeabilized state in the cell membrane disrupted by nsEP (20 or 40 pulses, 300 ns width, 7 kV/cm). We employed a LentiArray CRISPR library to generate knockouts (KOs) of 316 genes encoding for membrane proteins in U937 human monocytes stably expressing Cas9 nuclease. The extent of membrane permeabilization by nsEP was measured by the uptake of Yo-Pro-1 (YP) dye and compared to sham-exposed KOs and control cells transduced with a non-targeting (scrambled) gRNA. Only two KOs, for SCNN1A and CLCA1 genes, showed a statistically significant reduction in …

Opioid Use Disorder Prediction Using Machine Learning Of Fmri Data, A. Temtam, Liangsuo Ma, F. Gerard Moeller, M. S. Sadique, K. M. Iftekharuddin, Khan M. Iftekharuddin (Ed.), Weijie Chen (Ed.)

Electrical & Computer Engineering Faculty Publications

According to the Centers for Disease Control and Prevention (CDC) more than 932,000 people in the US have died since 1999 from a drug overdose. Just about 75% of drug overdose deaths in 2020 involved Opioid, which suggests that the US is in an Opioid overdose epidemic. Identifying individuals likely to develop Opioid use disorder (OUD) can help public health in planning effective prevention, intervention, drug overdose and recovery policies. Further, a better understanding of prediction of overdose leading to the neurobiology of OUD may lead to new therapeutics. In recent years, very limited work has been done using statistical …

Ict Security Tools And Techniques Among Higher Education Institutions: A Critical Review, Miko Nuñez, Xavier-Lewis Palmer, Lucas Potter, Chris Jordan Aliac, Lemuel Clark Velasco

Electrical & Computer Engineering Faculty Publications

Higher education institutions (HEIs) are increasingly relying on digital technologies for classroom and organizational management, but this puts them at higher risk for information and communication (ICT security attacks. Recent studies show that HEIs have experienced more security breaches in ICT security composed of both cybersecurity an information security. A literature review was conducted to identify common ICT security practices in HEIs over the last decade. 11 journal articles were profiled and analyzed, revealing threats to HEIs’ security and protective measures in terms of organizational security, technological security, physical security, and standards and frameworks. Security tools and techniques were grouped …

Non-Traditional Adult Learners After Covid-19: Applying National Standards For Online Teaching In Human Service Education, Chaniece Winfield, Kathryn Hughes, Jessica L. Huffman

Counseling & Human Services Faculty Publications

After the COVID-19 pandemic, the field of higher education increased its attention on quality distance learning for traditional and non-traditional adult learners. While the world saw an increase in online education enrollment as a result of the pandemic, non-traditional adult learners have historically relied on this method of education delivery to support their success in the collegiate setting. As the field of human service education supports the offering of distance learning instruction, it is vital for human service educators to consider the National Standards for Quality Online Teaching when educating adult learners. The authors of this article provide an overview …

The Effect Of Metalation On Antimicrobial Piscidins Imbedded In Normal And Oxidized Lipid Bilayers, Ana Dreab, Craig A. Bayse

Chemistry & Biochemistry Faculty Publications

Metalation of the N-terminal Amino Terminal Cu(II)- and Ni(II)-binding (ATCUN) motif may enhance the antimicrobial properties of piscidins. Molecular dynamics simulations of free and nickelated piscidins 1 and 3 (P1 and P3) were performed in 3 : 1 POPC/POPG and 2.6 : 1 : 0.4 POPC/POPG/aldo-PC bilayers (POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine: POPG, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol; aldo-PC, 1-palmitoyl-2-(9′-oxo-nonanoyl)-sn-glycero-3-phosphocholine) bilayer models. Nickel(II) binding decreases the conformation dynamics of the ATCUN motif and lowers the charge of the N-terminus to allow it to embed deeper in the bilayer without significantly changing the overall depth due to interactions of the charged half-helix …

Employee Green Behavior As The Core Of Environmentally Sustainable Organizations, Hannes Zacher, Cort W. Rudolph, Ian M. Katz

Psychology Faculty Publications

Environmental sustainability has become an ethical and strategic imperative for organizations, and more and more employees are interested, encouraged, or instructed to act in environmentally sustainable ways. Consequently, organizational scholars have increasingly studied individual-level antecedents of employee pro-environmental or employee green behavior (EGB). We argue that, to advance this literature and to inform effective interventions, research should investigate how EGB, as a compound performance domain, is associated with antecedents and consequences at multiple levels (i.e., individual, team, work context, organization, society). Accordingly, we pursue three interrelated goals with this review. We first present a comprehensive review of research on EGB, …

Navigating Identity Uncertainty: Identity Distress During The Covid-19 Pandemic, Alan Meca, Kelsie K. Allison, Julia Passini, Taryn Veniegas, Bethany Cruz, Linda G. Castillo, Seth J. Schwartz, Byron L. Zamboanga, Minas Michikyan, Melissa Bessaha, Pamela C. Regan, Kaveri Subrahmanyam, John Bartholomew, Brandy Piña-Watson, Miguel Ángel Cano, Charles R. Martinez Jr.

Psychology Faculty Publications

The long-term effects of the COVID-19 pandemic have only recently begun to be explored. Among college students, who were faced with sudden and unprecedented changes and challenges, it is likely that COVID-19 detrimentally impacted the establishment of a sense of self, a key developmental task of the college years. However, no research has examined the relationships among COVID-19 related worries, identity distress, and psychological and academic adjustment. To address these gaps in the current study, we examined the prevalence of identity distress, the relationship between COVID-19 related worries and identity distress, and the direct and indirect associations between COVID-19 related …

Trauma Exposure And Transdiagnostic Distress: Examining Shared And Ptsd-Specific Associations, Michael L. Crowe, Sage E. Hawn, Erika J. Wolf, Terence M. Keane, Brian P. Marx

Psychology Faculty Publications

Dimensional models of psychopathology suggest that the causes and consequences of psychopathology are attributable to a combination of syndrome specific and transdiagnostic features. There is considerable evidence that trauma exposure confers risk for a wide range of psychiatric conditions, yet no previous work has specifically examined the higher-order effects of trauma exposure within a structural model. We examined transdiagnostic and PTSD-specific associations with multiple forms of trauma exposure within a nation-wide sample (N = 1,649; 50% female) of military Veterans over-selected for posttraumatic stress disorder (PTSD). A higher-order Distress variable was estimated using PTSD, major depressive disorder (MDD), and generalized …

Ptsd And Alcohol Use Disorders Predict The Pace Of Cellular Aging, Sage E. Hawn, Xiang Zhao, Mark W. Miller, Sara Wallander, Christine Govan, Anjanette Stone, Steven A. Schichman, Mark W. Logue, Erika J. Wolf

Psychology Faculty Publications

Advanced epigenetic age is associated with psychopathology and may help to explain the link between psychopathology and physical health morbidity and mortality. Using a longitudinal sample of 171 trauma-exposed Veterans, we modeled the rate of change in epigenetic age across two time points (averaging 5.58 years apart) using two epigenetic age algorithms (GrimAge and Horvath) and tested associations with posttraumatic stress disorder (PTSD), alcohol use disorder (AUD), and depression. Results showed that PTSD (β = .199) and AUD (β = .186) were associated with a quickened pace of epigenetic aging over time (ps < .021). Results replicate and extend prior work and offer foundational support for identifying interventions that slow the pace of biological aging among those with psychopathology.

Criterion Validity Of Protective Behavioral Strategies For Alcohol Consumption Among College Students, Abby L. Braitman, Amy Stamates, Melissa Colangelo, Sarah J. Ehlke, Jordan Ortman, Kristin E. Heron, Kate B. Carey

Psychology Faculty Publications

Background: Protective behavioral strategies (PBS), or behaviors used to reduce harm associated with alcohol use, are often associated with lower levels of alcohol consumption, lower engagement in high-risk drinking behaviors, and fewer alcohol-related consequences. Although the majority of studies have found significant associations between higher PBS use and lower consumption or consequences, some studies have found nonsignificant or even positive associations. One explanatory hypothesis is that the mixed findings are due to differential content in PBS measures. Objectives: The current study examined the criterion validity of two widely-used PBS measures, the PBSS and the SQ. In a multi-institution online …

Dfhic: A Dilated Full Convolution Model To Enhance The Resolution Of Hi-C Data, Bin Wang, Kun Liu, Yaohang Li, Jianxin Wang

Computer Science Faculty Publications

Motivation: Hi-C technology has been the most widely used chromosome conformation capture(3C) experiment that measures the frequency of all paired interactions in the entire genome, which is a powerful tool for studying the 3D structure of the genome. The fineness of the constructed genome structure depends on the resolution of Hi-C data. However, due to the fact that high-resolution Hi-C data require deep sequencing and thus high experimental cost, most available Hi-C data are in low-resolution. Hence, it is essential to enhance the quality of Hi-C data by developing the effective computational methods.

Results: In this work, we propose …

Intergenic Transcription In In Vivo Developed Bovine Oocytes And Pre-Implantation Embryos, Saurav Ranjitkar, Mohammad Shiri, Jiangwen Sun, Xiuchun Tian

Computer Science Faculty Publications

Background

Intergenic transcription, either failure to terminate at the transcription end site (TES), or transcription initiation at other intergenic regions, is present in cultured cells and enhanced in the presence of stressors such as viral infection. Transcription termination failure has not been characterized in natural biological samples such as pre-implantation embryos which express more than 10,000 genes and undergo drastic changes in DNA methylation.

Results

Using Automatic Readthrough Transcription Detection (ARTDeco) and data of in vivo developed bovine oocytes and embryos, we found abundant intergenic transcripts that we termed as read-outs (transcribed from 5 to 15 kb after TES) and …

Hashes Are Not Suitable To Verify Fixity Of The Public Archived Web, Mohamed Aturban, Martin Klein, Herbert Van De Sompel, Sawood Alam, Michael L. Nelson, Michele C. Weigle

Computer Science Faculty Publications

Web archives, such as the Internet Archive, preserve the web and allow access to prior states of web pages. We implicitly trust their versions of archived pages, but as their role moves from preserving curios of the past to facilitating present day adjudication, we are concerned with verifying the fixity of archived web pages, or mementos, to ensure they have always remained unaltered. A widely used technique in digital preservation to verify the fixity of an archived resource is to periodically compute a cryptographic hash value on a resource and then compare it with a previous hash value. If the …