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Life History Of Stranded Bottlenose Dolphins (Tursiops Truncatus) In Virginia, Margaret Cook Lynott

Biological Sciences Theses & Dissertations

Little is known about the life history and overall ecology of bottlenose dolphins that strand in Virginia. In this study, I examined archived life history samples and stranding data from bottlenose dolphins found in Virginia waters to (1) develop growth curves based on ages from sectioned teeth, (2) document characteristics of male and female reproductive organs, and (3) define the relationship between age/length and presence of neonatal characteristics during the first year of life. The Gompertz growth model provided the best fit of growth data for stranded T. truncatus in Virginia. Length at birth and asymptotic length for males were …

Nonosecond Pulsed Electric Field Induced Changes In Dielectric Properties Of Biological Cells, Jie Zhuang

Electrical & Computer Engineering Theses & Dissertations

Nanosecond pulsed electric field induced biological effects have been a focus of research interests since the new millennium. Promising biomedical applications, e.g. tumor treatment and wound healing, are emerging based on this principle. Although the exact mechanisms behind the nanosecond pulse-cell interactions are not completely understood yet, it is generally believed that charging along the cell membranes (including intracellular membranes) and formation of membrane pores trigger subsequent biological responses, and the number and quality of pores are responsible for the cell fate. The immediate charging response of a biological cell to a nanosecond pulsed electric field exposure relies on the …

Synergistic Effect Of Subnanosecond Pulsed Electric Fields And Temperature On The Viability Of Biological Cells, James Thomas Camp

Electrical & Computer Engineering Theses & Dissertations

Pulsed electric fields have been used to induce a biological response in cells, and at sufficient energy, can cause cell death. By reducing the pulse duration from presently used nanosecond to subnanosecond ranges, the electric field can be delivered to biological tissue non-invasively by the use of an antenna instead of electrodes, such as needles. Studies have previously been completed in which the aim was to determine the energy density (electric field strength, number of pulses) required to induce cell death with 800 ps pulses. Based on this data, it was concluded that for pulse durations of 200 ps, with …

Direct Current Electrokinetic Particle Transport In Micro/Nano-Fluidics, Ye Ai

Mechanical & Aerospace Engineering Theses & Dissertations

Electrokinetics has been widely used to propel and manipulate particles in micro/nano-fluidics. The first part of this dissertation focuses on numerical and experimental studies of direct current (DC) electrokinetic particle transport in microfluidics, with emphasis on dielectrophoretic (DEP) effect. Especially, the electrokinetic transports of spherical particles in a converging-diverging microchannel and an L-shaped microchannel, and cylindrical algal cells in a straight microchannel have been numerically and experimentally studied. The numerical predictions are in quantitative agreement with our own and other researchers' experimental results. It has been demonstrated that the DC DEP effect, neglected in existing numerical models, plays an important …

Estimating The Impact Of Human Papillomavirus (Hpv) Vaccination On Hpv Prevalence And Cervical Cancer Incidence In Mali, Leree Tracy, Holly Gaff, Colleen Burgess, Samba Sow, Patti E. Gravitt, J. Kathleen Tracy

Biological Sciences Faculty Publications

Human papillomavirus vaccines have potential to reduce cervical cancer incidence and mortality; however, cultural and economic barriers may hinder success in developing countries. We assessed impact of a single vaccine campaign in Mali with use of mathematical modeling. Our model shows that decreases in the prevalence of Human papillomavirus infection are proportional to achieved vaccination coverage.

The Innovation Paradox: How Innovation Products Threaten The Innovation Process, Scott Francisco

Reconstruction: Studies in Contemporary Culture

Innovation is a growing trend and real concern for the economy, environment, human health and national security. Innovation outputs are prolific, while continuous research advances strategies for innovation success. Little if any research, however, connects the products>/i> and the process of innovation, and explores the impact each is having on the other. This paper proposes that the products of innovation today, particularly ICT devices, networks, databases and analytics, are themselves attacking the foundations of innovation process and skills. Research on these core innovation skills, creativity, risk-taking and mental development find them best cultivated in a hands-on, socio-spatial environments. This …

A Test Of The Area Of Refuge Hypothesis In Indo-Pacific Marine Biogeography, Jonnell C. Sanciangco

Biological Sciences Theses & Dissertations

Many hypotheses have been proposed to explain the marine biogeographic patterns of species richness of the Indo-Pacific. One of these hypotheses suggests that available habitat area is the main predictor of species richness. This is the basis for the area of refuge hypothesis that attempts to explain the global maxima of species richness found in the Coral Triangle, the area encompassing mostly the Philippines, Indonesia, and Malaysia. This species-area effect also has habitat heterogeneity as a potential component. The purpose of this study is to test the effects of habitat availability predictors, such as shallow water area and coastline length …

Light Environment And Seasonal Dynamics Of Microalgae In The Annual Sea Ice At Terra Nova Bay, Ross Sea, Antarctica, L. Lazzara, I. Nardello, C. Ermanni, O. Mangoni, V. Saggiomo

OES Faculty Publications

We investigated the physical conditions of the Spring pack ice environment at Terra Nova Bay to understand their influence on the structure and physiology of sympagic microalgae. Bio-optical methods were used to study the availability and spectral quality of solar radiation, both inside and underneath the ice cover. Pack ice thickness was around 2.5 m, with a temperature between -2 and -7°C. On average, only 1.4% of surface PAR penetrated to the bottom ice and less than 0.6% below platelet ice level. Surface UV-B radiation under the bottom ice was 0.2-0.4%. Biomass concentrations up to 2400 mg Chl a m- …

A Study Of The Seasonal Composition And Abundance Of Phytoplankton And Autotrophic Picoplankton In A Brackish Water Lake In Portsmouth Virginia, Jennifer Leigh Wolny

Biological Sciences Theses & Dissertations

The phytoplankton and autotrophic picoplankton populations of Hoffler Lake, a brackish-water lake in Portsmouth, Virginia, were monitored from May 1997 through May 1998, Analyses of the phytoplankton community using the Utennohl method showed a dominance of Chlorophytes (61-88% of the total abundance) throughout the year, including a winter bloom of Chlamydomonas snowii (maximum concentration of 2.5 x 101 cells/L). Subdominants were Cyanobacteria (10-33% of the total abundance) whose composition included several species of Anabaena, Lyngbya, and a fall bloom of Microcoleus sp. Diatoms, dinoflagellates, and cryptophytes played a secondary role in the phytoplankton community of Hoffler Lake. …

Developmental And Hormonal Regulation Of The Expression Of Cytosolic Phospholipase A2 Protein Levels In Baboon Placenta, Kathleen Elizabeth Dunstan

Chemistry & Biochemistry Theses & Dissertations

Cytosolic phospholipase A₂ ( cPLA₂) specifically catalyzes the release of arachidonate from membrane phospholipids, thus initiating synthesis of prostaglandins, thromboxanes, and other eicosanoids. The human and nonhuman primate placenta actively synthesize high levels of eicosanoids which play both an autocrine and paracrine role in placental function. Previous studies in Dr. Miriam D. Rosenthal's laboratory have shown that cPLA₂ mRNA levels are developmentally regulated. The present study was designed to determine whether placental cPLA₂ protein levels increased during gestation. Since the marked increases in estrogen levels during normal pregnancy have been shown to enhance morphological and …

Velocity Estimation Via A Neural Network Enhanced By Classical Detection Algorithms, Zeki Berk Hamşioğlu

Electrical & Computer Engineering Theses & Dissertations

The goal of this research is to show how to solve a velocity estimation problem using a neural network connected to an array of sensors. Motivated by biological studies involving insect vision, the neural network utilized is a member of a class of shunting neural networks. When an object moves across the face of the sensor array, the neural network's pulse response is first temporally located using classical M-ary detection techniques. Both the deterministic and stochastic cases are considered. Then the network's pulse response is post-processed via an existing velocity estimation algorithm based on a Volterra series model of the …

Microwave Plasma Chemical Vapor Deposited Diamond Tips For Scanning Tunneling Microscopy, Sacharia Albin, Jianli Zheng, John B. Cooper, Weihai Fu, Arnel C. Lavarias

Electrical & Computer Engineering Faculty Publications

Diamond microparticles were grown on etched tungsten wires using a microwave plasma-assisted chemical vapor deposition process. The apexes on cubo-octahedral particles bound by {100} and {111} facets were effectively used as tunneling tips for scanning tunneling microscopy. The atomically resolved surface image of highly oriented pyrolytic graphite was acquired. Tunneling characteristics revealed a higher electron emission from the diamond tips than that from the platinum–iridium tips. The same diamond tips were used to produce surface indentation and its image. © 1997 American Institute of Physics.

Diamond For Fiber-Optic Raman Spectroscopy, Jianli Zheng

Electrical & Computer Engineering Theses & Dissertations

Raman spectroscopy is a powerful technique for molecular structural studies and concentration measurement. Being a single beam technique, the Raman intensity depends on the optical power of the excitation source, optical alignment of the system, detector response as well as species concentration. To eliminate the unwanted variations in the system and get the concentration information of the species, intensity referencing is necessary. The existing referencing methods either use a constant Raman band of the sample, or add a chemically inert material with known concentration into the sample. Unfortunately, the former method is not always applicable and the latter is not …

Phylogenetic Diversity Of The Bacterial Community From A Microbial Mat At An Active, Hydrothermal Vent System, Loihi Seamount, Hawaii, Craig L. Moyer, Fred C. Dobbs, David M. Karl

OES Faculty Publications

The phylogenetic diversity of small-subunit rRNA genes associated,vith the domain Bacteria was examined (by using previously defined operational taxonomic units [C. L. Moyer, F. C. Dobbs, and D. M. Karl, Appl. Environ. Microbiol. 60:871-879, 1994]; those for Pele's Vents Bacteria are hereafter abbreviated PVB OTUs) with samples from a microbial mat at an active, deep-sea hydrothermal vent system. A cluster of phylogenetically related PVB OTUs (OTUs 2, 3, 6, and 8) was closely affiliated with Thiovulum sp. contained within the epsilon subclass of the class Proteobacteria and accounted for 60.5% of the small-subunit rRNA bacterial clone library from Pele's Vents. …

Chesapeake Bay Phytoplankton: I. Composition, Harold G. Marshall

Biological Sciences Faculty Publications

A list of 708 Phytoplankton taxa from 10 algal divisions has been compiled for the Chesapeake Bay and is based on the analysis of 2384 water samples over three decades of study.

The Isolation And Identification Of Polyphenolics From Grape Seeds And Their Activity Towards Specific Cell Lines, Joseph A. Reddy

Chemistry & Biochemistry Theses & Dissertations

Grape seed extract is being marketed in France for cardiovascular use and skin care. The active constituents of both these drugs are certain polyphenols called procyanidins. A similar seed extract has now been obtained from Virginia Chardonnay grape seeds. Ten of the major components in this extract have been isolated in their pure form by the semi-preparative HPLC separation on a YMC ODS-AQ (Octadecylsilane-AQ) column. The ODS-Aq column has been shown to have a better resolving capacity compared to the Spherisorb ODS (Octadecylsilane) column previously described in the literature. This has been attributed to the ODSAQ support which has both …

Phytoplankton Relationships To Water Quality In Lake Drummond And Two Drainage Ditches, Christine G. Phillips, Harold G. Marshall

Biological Sciences Faculty Publications

A twelve-month phytoplankton study was conducted in Lake Drummond and Washington and Jericho Ditches from December 1988 to November 1989. Four dominant phytoplankton groups were identified at these sites. These were the Bacillariophyceae, Cyanophyceae, Cryptophyceae and an autotrophic picoplankton component. Over the past 20 years there has been a decrease in the mean pH levels of Lake Drummond and the replacement of one its former major components, the Chlorophyceae, by the Cyanophyceae. Based on water quality analysis results and species diversity indices, Lake Drummond is classified as in an early eutrophic stage of development.

Embryonic Chicken Fibroblast Collagen Binding Proteins: Distribution, Role In Substratum Adhesion, And Relationship To Integrins, Roy C. Ogle, A. Jeannette Potts, Marchall Yacoe, Charles D. Little

School of Medical Diagnostics & Translational Sciences Publications

Collagen binding proteins (CBP) are hydrophobic, cell surface polypeptides, isolated by collagen affinity chromatography. Antibodies to CBPs inhibit the attachment of embryonic chicken heart fibroblasts to native type I collagen fibrils in a dose-dependent manner. The CBP antibodies also induce rounding and detachment of cells adherent to a planar substratum. This process of antibody-mediated substratum detachment resulted in a clustering of CBP and cell-associated extracellular matrix at the cell surface, and the rearrangement of filamentous actin. Other functional studies showed that cells grown within a three-dimensional gel of type I collagen cannot be immunostained at the cell surface with CBP …

Spectroscopic Study Of Decomposition Products Of Gossypol, David Bernard Hankins

Chemistry & Biochemistry Theses & Dissertations

Basic research into reproductive physiology has begun to identify possible new approaches to controlling fertility. Gossypol, a natural polyphenol compound isolated from cottonseed oil, has been shown to be an effective male antifertility agent. The decomposition products of gossypol which are thought to possess more potent antifertility effects than the parent compound gossypol have been studied extensively in more recent research. The purpose of this research is to develop techniques and methods which could be used to identify decomposition products of gossypol. A model decomposition reaction by which a smaller molecule can be isolated in a pure state has been …

The Effect Of Intuition On Creativity As Discussed By Several Individuals Of Creative Genius, Mary Jean Campbell

Institute for the Humanities Theses

When the term intuition came into psychological parlance less than a century ago, the argument began over whether intuition could affect creativity, and whether it, in fact, existed at all as part of cognitive process. This paper will begin with a discussion of the meaning of intuition and some of the implications of that term, address the difficulties inherent in relying on self-report for information, and then turn to the statements of several well-known creative individuals about the role of intuition or intuition-related phenomena in their work.

The selection of creative individuals will be limited to those who have attained …

A Model Of The Population Dynamics Of The Blue Crab In Chesapeake Bay, Betty Springer Hester

OES Theses and Dissertations

This study has particular application to the blue crab fisheries in Chesapeake Bay, an economically important industry whose successful management has been hindered by relatively poor understanding of the population dynamics. Recent biological studies have indicated a systematic spawning behavior which seems designed for ejection of larvae seaward from the bay entrance where they spend their pre-metamorphosis stages in the neuston. A physical mechanism for retention of the larvae in sufficient proximity to the bay entrance for their return at the proper time which involves the action of wind stress in shallow waters is proposed. Since the supply of blue …

Aspects Of Larval Ecology Of Squilla Empusa (Crustacea, Stomatopoda) In Chesapeake Bay, Steven G. Morgan

OES Faculty Publications

Larvae of Squilla empusa were collected from the plankton and were laboratory-reared in 16 combinations of temperature and salinity to determine their tolerances. Larvae survived longer and molted more frequently when reared at 25%, and 20° or 25° C, which corresponds to the natural conditions of Chesapeake Bay when the larvae were collected.

A 2 year planktonic survey conducted in the lower region of the bay by the Virginia Institute of Marine Sciences was compared with a survey made at the bay mouth in 1976. The seasonal occurrence of Squilla empusa larvae extended from the last week of July until …

Ocean Chlorophyll Studies From A U-2 Aircraft Platform, Hongsuk H. Kim, Charles R. Mcclain, Lamdin R. Blaine, William D. Hart, Larry P. Atkinson, James A. Yoder

CCPO Publications

Chlorophyll gradient maps of large ocean areas were generated from U-2/OCS data obtained over test sites in the Pacific and the Atlantic Oceans. The delineation of oceanic features using the upward radiant intensity relies on an analysis method which presupposes that radiation backscattered from the atmosphere and the ocean surface can be properly modeled by using a measurement made at 778 nm. The calculation of atmospheric radiance was performed by using a method developed by J.V. Dave. An estimation of the chlorophyll concentration is performed by properly ratioing radiances measured at 472 and 548 nm after removing the atmospheric effects. …

Seasonal Composition Of Zooplankton Of Lower Chesapeake Bay And Virginia Coastal Waters, Elizabeth W. Atkinson

Biological Sciences Theses & Dissertations

Abstract unavailable.

A Physical Hydrographic Study Of The Lafayette River, E. Glenn White

OES Theses and Dissertations

A basic investigation of the physical ~properties, temperature, density and current velocity, in the Lafayette River is presented. Graphs of the observations indicata a difference in salinity, density and current velocity- distributions after moderate to heavy rains from the graphs during a dry period. The changes in distribution indicate a change in the classification of the estuary from a type B/C, partially mixed estuary, to a type D, sectionally homogeneous estuary. The estuary is predominantly a well-mixed or sectionally homogeneous estuary.

Changes in salinity, temperature, density and current velocity over tidal cycles are discussed as well as tidal height data. …

Diurnal Distribution Of Phytoplankton From A Single Station At The Mouth Of The James River, Harold G. Marshall

Biological Sciences Faculty Publications

Eight sets of water samples were taken to a depth of 36 ft. during a 24-hr. period. A homogeneity was found in the vertical distribution of the total phytoplankton in samples from 7 of the 8 hydro-casts. Skeletonema costatum and Asterionella japonica were the most numerous phytoplankters in every sample, with the majority of their counts each exceeding 500, 000 cells per liter.

The Distribution Of Phytoplankton Along A 140 Mile Transect In The Chesapeake Bay, Harold G. Marshall

Biological Sciences Faculty Publications

The composition of phytoplankton progressively changed in the surface samples taken between Norfolk, Virginia and Cambridge, Maryland. These was an inverse relationship between the numbers of phytoplankters and zooplankters in the transect samples. Vertical stratification of the plankton was studied at two 24-hr, stations at the Great Wicomlco and Patuxent Rivers. The vertical centers of zooplankton and phytoplankton populations fluctuated over the study period and evidence is given to support a grazing phenomenon. Large concentrations of nannoplankters were found in all the plankton samples taken and is attributed to the collection procedure using the Van Dorn sampler.

The Voyager, 1947, Norfolk Division Of The College Of William And Mary

University Yearbooks

1947 yearbook of the Norfolk Division of the College of William and Mary (now, Old Dominion University)