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Articles 3811 - 3840 of 7452
Full-Text Articles in Entire DC Network
Motherhood Redefined: An Interpretative Phenomenological Analysis Of The Lived Experiences Of Lesbian Mothers And The Sociopolitical Conflicts That Shape Their Narratives, Melissa Miller-Munoz
Motherhood Redefined: An Interpretative Phenomenological Analysis Of The Lived Experiences Of Lesbian Mothers And The Sociopolitical Conflicts That Shape Their Narratives, Melissa Miller-Munoz
Department of Conflict Resolution Studies Theses and Dissertations
This study explored the lives of lesbian mothers with children conceived through insemination or the adoption process in an effort to understand the conflicts they encountered along their journey to motherhood and how they managed those conflicts. The qualitative study included in-depth interviews conducted with six participants. The participants’ ages ranged between 25-60 years old. Interview results were analyzed to explore participants’ narratives in regards to their experiences, relationships, identities and transformation into motherhood. This research highlights significant ongoing developments in the field of lesbian, gay, bisexual, transgender and queer (LGBTQ) studies and seeks to intersect the boundaries between interpersonal …
A Phenomenological Study: Foster Care Youth Aging Out Of The System, Guy Thompson
A Phenomenological Study: Foster Care Youth Aging Out Of The System, Guy Thompson
Theses and Dissertations
The purpose of this applied dissertation was to explore the lived experiences of teens aging out of foster care, in an effort to better understand their needs. While the child welfare system is geared towards family preservation, reunification, and adoption, most young adults transitioning from the foster care system are not reunited with family members or are only reunited as a last resort. Unfortunately, every year, thousands are leaving the support of the foster care system, unprepared. Youth departing the child welfare system are not gradually transitioned into becoming self-sufficient. They do not have an effective support system available if …
Social Worker Experiences Serving At-Risk Youth With Emotional And Behavioral Disabilities, Sherriese S. Anderson
Social Worker Experiences Serving At-Risk Youth With Emotional And Behavioral Disabilities, Sherriese S. Anderson
Theses and Dissertations
This dissertation was a qualitative study of school social workers who worked with special needs adolescent youth with emotional and behavioral disabilities in a public high school. This study centered on the experiences of 10 social workers in a public high school in New Jersey. All 10 school social workers were interviewed individually to uncover (a) how they perceived the school social worker's role within the public school setting, (b) the lived experiences of school social workers who worked with special needs youth with emotional and behavioral disabilities, and (c) why they desired to continue working with at-risk special needs …
Data Collection Challenges And Recommendations For Early Career Researchers, Marylen Rimando, Andrea M. Brace, Apophia Namageyo-Funa, Tiffany L. Parr, Diadrey-Anne Sealy, Teaniese L. Davis, Lourdes M. Martinez, Richard W. Christiana
Data Collection Challenges And Recommendations For Early Career Researchers, Marylen Rimando, Andrea M. Brace, Apophia Namageyo-Funa, Tiffany L. Parr, Diadrey-Anne Sealy, Teaniese L. Davis, Lourdes M. Martinez, Richard W. Christiana
The Qualitative Report
Data collection is critical to the social research process. When implemented correctly, data collection enhances the quality of a social research study. However, doctoral students and early career researchers may encounter challenges with data collection. This article reports on the data collection challenges in dissertation research encountered by doctoral students enrolled in a public health program at a southeastern United States urban university. Each doctoral student shared at least one challenge and how it affected the data collection process. Additionally, the doctoral students shared how the identified challenges were addressed or suggested recommendations. Understanding these experiences of doctoral students is …
Qualitative Contributions To A Randomized Controlled Trial Addressing Hiv/Aids-Stigma In Medical Students, Melissa Marzán-Rodríguez, Nelson Varas-Diaz, Torsten Neilands
Qualitative Contributions To A Randomized Controlled Trial Addressing Hiv/Aids-Stigma In Medical Students, Melissa Marzán-Rodríguez, Nelson Varas-Diaz, Torsten Neilands
The Qualitative Report
Specialized training for healthcare professionals (HCP) in order to reduce HIV/AIDS related stigma must be part of a public health model for HIV/AIDS. Tested interventions to reduce HIV/AIDS related stigma among HCP have been mostly absent from these efforts. A qualitative approach was used to assess stigma reduction within a traditional randomized controlled design in order to better understand how our current stigma intervention worked and was understood by 2nd year medical students. After conducting a quantitative follow up survey one-year post intervention we conducted 20 in-depth qualitative interviews with a subsample of our intervention group participants as part of …
The Our Florida Reefs Coastal Use Survey: An Online Survey To Support Stakeholder Management Recommendations For Southeast Florida, Amanda Rose Costaregni
The Our Florida Reefs Coastal Use Survey: An Online Survey To Support Stakeholder Management Recommendations For Southeast Florida, Amanda Rose Costaregni
HCNSO Student Theses and Dissertations
Coral reefs are an important resource world-wide. Unfortunately, coral reef conditions are declining in many areas due to both global and local stressors. The objective of this study was to survey stakeholders in southeast Florida to better understand reef use in the region. Stakeholders spatially identified where and how often they conducted their activities. These data were compiled and analyzed in GIS to determine spatial use patterns. Both location and intensity of use were analyzed to determine which areas may be under greater stress from recreational activities. It was found that reef use was not evenly distributed in the region …
Natural Vs. Anthropogenic Sedimentation: Does Reducing A Local Stressor Increase Coral Resilience To Climate Change?, Francesca Fourney
Natural Vs. Anthropogenic Sedimentation: Does Reducing A Local Stressor Increase Coral Resilience To Climate Change?, Francesca Fourney
HCNSO Student Theses and Dissertations
Corals face serious worldwide population declines due to global climate change in combination with direct anthropogenic impacts. Global climate change is difficult to manage locally, but policy makers can regulate the magnitude of local stressors affecting reefs. The objective of this experiment is to investigate if reducing sedimentation will enable reef corals to better endure global climate change. It has been shown that some coral species can handle climate change stress when provided with additional energy resources. Here I tested if the capacity of corals to cope with climate change can be improved when their ability to feed and photosynthesize …
Direct Phenotypic Screening In Mice: Identification Of Individual, Novel Antinociceptive Compounds From A Library Of 734 821 Pyrrolidine Bis-Piperazines, Richard A. Houghten, Michelle L. Ganno, Jay P. Mclaughlin, Colette T. Dooley, Shainnel O. Eans Torrey Pines Institute For Molecular Studies, Radleigh Santos, Travis Lavoi, Adel Nefzi, Greg Welmaker, Marc A. Giulianotti, Lawrence Toll
Direct Phenotypic Screening In Mice: Identification Of Individual, Novel Antinociceptive Compounds From A Library Of 734 821 Pyrrolidine Bis-Piperazines, Richard A. Houghten, Michelle L. Ganno, Jay P. Mclaughlin, Colette T. Dooley, Shainnel O. Eans Torrey Pines Institute For Molecular Studies, Radleigh Santos, Travis Lavoi, Adel Nefzi, Greg Welmaker, Marc A. Giulianotti, Lawrence Toll
Mathematics Faculty Articles
The hypothesis in the current study is that the simultaneous direct in vivo testing of thousands to millions of systematically arranged mixture-based libraries will facilitate the identification of enhanced individual compounds. Individual compounds identified from such libraries may have increased specificity and decreased side effects early in the discovery phase. Testing began by screening ten diverse scaffolds as single mixtures (ranging from 17 340 to 4 879 681 compounds) for analgesia directly in the mouse tail withdrawal model. The “all X” mixture representing the library TPI-1954 was found to produce significant antinociception and lacked respiratory depression and hyperlocomotor effects using …
A Comparison Of Infaunal Community Structure Between Pre- And Post Construction Sampling Of Artificial Fdot Rock- Pile Reefs In Broward County, Florida, Joan Lorraine Guerra
A Comparison Of Infaunal Community Structure Between Pre- And Post Construction Sampling Of Artificial Fdot Rock- Pile Reefs In Broward County, Florida, Joan Lorraine Guerra
HCNSO Student Theses and Dissertations
Artificial reefs (ARs) are generally created with the intention of increasing local fish populations, biodiversity and corresponding habitat productivity, most often in support of commercial and recreational uses (e.g., diving and fishing). Numerous studies have investigated the communities that develop on artificial reefs. By contrast, far less research has focused on the surrounding infaunal communities, which represent critical trophic resources for many species that populate both natural and artificial reefs, and which may be affected both by AR deployment and the organisms that subsequently recruit to them. This study compared infaunal organism abundances at four sites between the inner and …
Genomic Legacy Of The African Cheetah, Acinonyx Jubatus, Pavel Dobrynin, Shiping Liu, Gaik Tamazian, Zijun Xiong, Andrey A. Yurchenko, Ksenia Krasheninnikova, Sergey Kliver, A. Schmidt-Kunzel, Klaus-Peter Koepfli, Warren E. Johnson, Lukas F. K. Kuderna, Raquel Garcia-Perez, Marc De Manuel, Ricardo Godinez, Aleksey Komissarov, Alexey Makunin, Vladimir Brukhin, Weilin Qiu, Long Zhou, Fang Li, Jian Yi, Carlos A. Driscoll, Agostinho Antunes, T. K. Oleksyk, Eduardo Eizirik, Polina Perelman, Melody E. Roelke, David E. Wildt, Mark Diekhans, Tomas Marques-Bonet, Laurie Marker, Jong Bhak, Jun Wang, Guojie Zhang, Stephen J. O'Brien
Genomic Legacy Of The African Cheetah, Acinonyx Jubatus, Pavel Dobrynin, Shiping Liu, Gaik Tamazian, Zijun Xiong, Andrey A. Yurchenko, Ksenia Krasheninnikova, Sergey Kliver, A. Schmidt-Kunzel, Klaus-Peter Koepfli, Warren E. Johnson, Lukas F. K. Kuderna, Raquel Garcia-Perez, Marc De Manuel, Ricardo Godinez, Aleksey Komissarov, Alexey Makunin, Vladimir Brukhin, Weilin Qiu, Long Zhou, Fang Li, Jian Yi, Carlos A. Driscoll, Agostinho Antunes, T. K. Oleksyk, Eduardo Eizirik, Polina Perelman, Melody E. Roelke, David E. Wildt, Mark Diekhans, Tomas Marques-Bonet, Laurie Marker, Jong Bhak, Jun Wang, Guojie Zhang, Stephen J. O'Brien
Biology Faculty Articles
Background
Patterns of genetic and genomic variance are informative in inferring population history for human, model species and endangered populations.
Results
Here the genome sequence of wild-born African cheetahs reveals extreme genomic depletion in SNV incidence, SNV density, SNVs of coding genes, MHC class I and II genes, and mitochondrial DNA SNVs. Cheetah genomes are on average 95 % homozygous compared to the genomes of the outbred domestic cat (24.08 % homozygous), Virunga Mountain Gorilla (78.12 %), inbred Abyssinian cat (62.63 %), Tasmanian devil, domestic dog and other mammalian species. Demographic estimators impute two ancestral population bottlenecks: one >100,000 years …
Connectivity Of Coastal And Oceanic Ecosystems: Pelagic Habitat Use By Juvenile Reef Fishes In The Gulf Of Mexico, Katie Bowen
Connectivity Of Coastal And Oceanic Ecosystems: Pelagic Habitat Use By Juvenile Reef Fishes In The Gulf Of Mexico, Katie Bowen
HCNSO Student Theses and Dissertations
The assemblage structure, abundance, biomass, and vertical distribution of juvenile reef fishes in the offshore pelagic habitat of the northern Gulf of Mexico are described as part of the NOAA-supported Offshore Nekton Sampling and Analysis Program. The results presented here are from a 3-month, continuous sampling series in 2011 in which discrete depth strata from 0 to 1500 m were sampled using a 10-m2 MOCNESS midwater trawl. This is the first study to examine pelagic juvenile reef fish distributions across the entire oceanic northern Gulf of Mexico seaward of the continental shelf break after the Deepwater Horizon Oil Spill. …
Feeding Ecology Of The Invasive Lionfish (Pterois Spp.) And Comparison With Two Native Species: Schoolmaster Lutjanus Apodus (Walbaum, 1792) And Graysby Cephalopholis Cruentata (Lacepède, 1802), Jesse J. Secord
HCNSO Student Theses and Dissertations
Predator satiation and prey-size preference were determined for locally caught lionfish, schoolmaster, and graysby, all co-occurring predatory fishes in the Florida coral reef ecosystem. Individuals were evaluated by exposing them to wild-caught killifish over a gradient of four size classes (20 mm to 60 mm, in 10 mm increments). Preference trials extended over a 2 hr time period and were filmed to determine the order in which each prey item was consumed. Satiation was evaluated by exposing the predators to an equal number of excess prey items for 24 hrs and evaluating consumed prey weight. Lionfish and schoolmaster showed a …
Faunal Composition And Distribution Of Pelagic Larval And Juvenile Flatfishes (Teleostei: Pleuronectiformes) In The Northern Gulf Of Mexico: Connectivity Between Coastal And Oceanic Epipelagic Ecosystems, Lacey Malarky
HCNSO Student Theses and Dissertations
Pleuronectiformes (flatfishes) occur throughout the global oceans, and have high ecological and commercial importance in some areas. Though much is known regarding life history, abundance, and distribution for the benthic adult stage of flatfish species, much less is known about the pelagic larval phases of flatfishes in the open ocean. Taxonomic uncertainty and limited sampling in the oceanic Gulf of Mexico contribute to data gaps with respect to the distribution of early life history stage of flatfishes in this region. Knowledge of the faunal composition, abundance and distribution of larval flatfishes, such as members of Bothus, which have extended …
The Effects Of Petroleum Pollutants On Sea Urchins Reproduction And Development, Kellie C. Pelikan
The Effects Of Petroleum Pollutants On Sea Urchins Reproduction And Development, Kellie C. Pelikan
HCNSO Student Theses and Dissertations
Disturbances, such as mass pollution events, threaten the health of vulnerable ecosystems. Recent media attention has focused on the devastating mass oil spills, but daily petroleum input from recreational and commercial ship bilge release has been overlooked. The focus of this study was the effect of petroleum products found in bilge water on fertilization success and larval viability of two sea urchin species, Lytechinus variegatus and Eucidaris tribuloides. Unlike other pollutant studies that have focused on sperm characteristics and concentrations, I chose to examine how egg integrity was compromised by petroleum products. Scanning electron microscopy revealed eggs were degraded when …
Evaluation Of Frictional Forces Between Brackets Of Different Types At Various Angulations And An Arch Wire: With And Without Pulsating Vibration, Benjamin M. Christman
Evaluation Of Frictional Forces Between Brackets Of Different Types At Various Angulations And An Arch Wire: With And Without Pulsating Vibration, Benjamin M. Christman
Student Theses, Dissertations and Capstones
Objective: The objective of this study was to determine the effect of pulsating vibration on the sliding resistance between orthodontic brackets and stainless steel wires. Brackets were placed at two different angulations (0° and 5°) to simulate leveling of a tipped tooth during tooth movement. Pulsating vibration was delivered via the AcceleDent device.
Background: Friction is defined as a force that retards or resists the relative motion of two objects in contact, and its direction is tangential to the common boundary of the two surfaces in contact. This has been of interest to the orthodontist since the mid-20th century. Since …
Habitat Utilization And Vertical Distribution Of The Great Barracuda Sphyraena Barracuda (Edwards 1771) In The Western North Atlantic Using Electronic Archival Tags, Noah R. Hansen, David W. Kerstetter
Habitat Utilization And Vertical Distribution Of The Great Barracuda Sphyraena Barracuda (Edwards 1771) In The Western North Atlantic Using Electronic Archival Tags, Noah R. Hansen, David W. Kerstetter
Marine & Environmental Sciences Faculty Articles
The Great Barracuda Sphyraena barracuda is a large predatory teleost commonly seen in the tropics of the Western North Atlantic. Using pop-up satellite archival tags (PSATs), two large Great Barracuda (101 and 104 cm FL) were tagged off South Florida for a 15-day deployment period. Great Barracuda 88094 traveled 471 km minimum straight-line distance (MSLD) over the deployment duration, while Great Barracuda 88095 traveled 1231 km MSLD. Great barracuda 88094 achieved a maximum depth of 145.2 m, while 88095 to a maximum depth of 186.9 m, although such movements were for short time durations. The data obtained indicate significant differences …
Semi-Automated Object-Based Classification Of Coral Reef Habitat Using Discrete Choice Models, Steven Saul, Samuel J. Purkis
Semi-Automated Object-Based Classification Of Coral Reef Habitat Using Discrete Choice Models, Steven Saul, Samuel J. Purkis
Marine & Environmental Sciences Faculty Articles
As for terrestrial remote sensing, pixel-based classifiers have traditionally been used to map coral reef habitats. For pixel-based classifiers, habitat assignment is based on the spectral or textural properties of each individual pixel in the scene. More recently, however, object-based classifications, those based on information from a set of contiguous pixels with similar properties, have found favor with the reef mapping community and are starting to be extensively deployed. Object-based classifiers have an advantage over pixel-based in that they are less compromised by the inevitable inhomogeneity in per-pixel spectral response caused, primarily, by variations in water depth. One aspect of …
The Current Volume 26 : Issue 14, Nova Southeastern University
The Current Volume 26 : Issue 14, Nova Southeastern University
Student Newspapers
No abstract provided.
Southeast Florida Large Coral Assessment 2015, Brian K. Walker, Katelyn Klug
Southeast Florida Large Coral Assessment 2015, Brian K. Walker, Katelyn Klug
Marine & Environmental Sciences Faculty Reports
The 2013 nearshore mapping project conducted by Walker and Klug expanded the previous knowledge on the amount, location, and species type of ecologically important large (>2 m) coral colonies in southeast Florida. They discovered over 110 previously undocumented large corals of which 60 were dead and 50 were still alive; 40 of the living corals were larger than 2 m wide and up to 5 m in diameter. Because these corals are the largest and oldest organisms on our reefs, they deserve special attention.
Currently there is unprecedented disease and bleaching in the northern portion of the Florida Reef …
The Mechanisms Of Moral Disengagement In George W. Bush’S “War On Terror” Rhetoric, Stefan Cartledge, Lorraine Bowman-Grieve, Marek Palasinski
The Mechanisms Of Moral Disengagement In George W. Bush’S “War On Terror” Rhetoric, Stefan Cartledge, Lorraine Bowman-Grieve, Marek Palasinski
The Qualitative Report
Despite considerable literature on the Bush administration’s war on terrorism rhetoric, little attention has been paid to its discourse of moral disengagement, leaving an important and still relevant gap that this paper aims to address. Rather than approaching this gap in terms of an archival historical analysis that is disconnected from the present, it proposes an exploratory revisit of the rhetoric that the benefits of hindsight might enrich and, we argue, aid in understanding connections to the current post-invasion turmoil and the gradual ISIS takeover. Having subjected nineteen presidential speeches to qualitative content analysis, we identified a number of moral …
Investigating The Role Of The Host Multidrug Resistance Associated Protein Transporter Family In Burkholderia Cepacia Complex Pathogenicity Using A Caenorhabditis Elegans Infection Model, Pietro Tedesco, Marco Visone, Ermenegilda Parrilli, Maria Luisa Tutino, Elena Perrin, Isabel Maida, Renato Fani, Francesco Ballestriero, Radleigh Santos, Clemencia Pinilla, Elia Di Schiavi, George Tegos, Donatella De Pascale
Investigating The Role Of The Host Multidrug Resistance Associated Protein Transporter Family In Burkholderia Cepacia Complex Pathogenicity Using A Caenorhabditis Elegans Infection Model, Pietro Tedesco, Marco Visone, Ermenegilda Parrilli, Maria Luisa Tutino, Elena Perrin, Isabel Maida, Renato Fani, Francesco Ballestriero, Radleigh Santos, Clemencia Pinilla, Elia Di Schiavi, George Tegos, Donatella De Pascale
Mathematics Faculty Articles
This study investigated the relationship between host efflux system of the non-vertebrate nematode Caenorhabditis elegans and Burkholderia cepacia complex (Bcc) strain virulence. This is the first comprehensive effort to profile host-transporters within the context of Bcc infection. With this aim, two different toxicity tests were performed: a slow killing assay that monitors mortality of the host by intestinal colonization and a fast killing assay that assesses production of toxins. A Virulence Ranking scheme was defined, that expressed the toxicity of the Bcc panel members, based on the percentage of surviving worms. According to this ranking the 18 Bcc strains were …
A Comparison Of Macroinfaunal Community Structure Between Artificial Concrete Boulder Reefs And Adjacent Natural Reefs In Broward County, Florida, Amber C. Metallo
A Comparison Of Macroinfaunal Community Structure Between Artificial Concrete Boulder Reefs And Adjacent Natural Reefs In Broward County, Florida, Amber C. Metallo
HCNSO Student Theses and Dissertations
Relatively little is known about either the biological (i.e., predation) or physical (i.e., current, sedimentation) effects that artificial reefs may have on surrounding benthic infaunal communities. Following deployment of artificial reefs (concrete boulders) between the first and second reefs off Fort Lauderdale, Florida, on 30 October 2009, sediment cores were taken at 4 distances along three replicate 10-m transects on 13 and 26 September 2013, and 24 and 25 May 2014 at each of four artificial reef sites and four of their adjacent natural reef sites using SCUBA. Infauna (>0.5mm) were extracted from the sediment and identified to the …
Pelagic Habitat Use By Juvenile Reef Fishes In The Gulf Of Mexico, Katie Bowen, Tracey Sutton
Pelagic Habitat Use By Juvenile Reef Fishes In The Gulf Of Mexico, Katie Bowen, Tracey Sutton
Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures
The assemblage composition, abundance, frequency of occurrence, and vertical distribution of juvenile reef fishes in the offshore pelagic habitat of the northern Gulf of Mexico are described. This study, a component of the NOAA-supported Offshore Nekton Sampling and Analysis Program, is the first to examine juvenile reef fish distributions across the oceanic northern Gulf of Mexico after the Deepwater Horizon oil spill. Results presented here are derived from a 3-month, spring/summer research cruise in 2011 on the M/V Meg Skansi. A 10-m2 MOCNESS midwater trawl was used to sample 45 stations from the surface to a depth of …
The Current Volume 26 : Issue 13, Nova Southeastern University
The Current Volume 26 : Issue 13, Nova Southeastern University
Student Newspapers
No abstract provided.
Field Recording Or Field Observation?: Audio Meets Method In Qualitative Research, Sam Smiley
Field Recording Or Field Observation?: Audio Meets Method In Qualitative Research, Sam Smiley
The Qualitative Report
The field observation, an ethnographic practice of collecting data and information about a given social setting and situation is often used in preliminary research to have an understanding of the community one is researching. However, from an artist/musician's perspective, the field observation has many commonalities with techniques used in audio field recording. How can field recording be used in parallel with field observations to explore and understand a community through art? This essay will begin with a comparison of field observations and field recordings as methods in their own disciplines, and continue with the concept of “attention” in art, music, …
Exploring Classroom Practices In Collectivist Cultures Through The Lens Of Hofstede’S Model, Amrita Kaur, Mohammad Noman
Exploring Classroom Practices In Collectivist Cultures Through The Lens Of Hofstede’S Model, Amrita Kaur, Mohammad Noman
The Qualitative Report
This study explores beliefs and classroom practices of teachers from collectivist nations through the lens of Hofstede’s cultural dimensions. In this grounded theory study, an in-depth investigation of the ways in which six teachers from five different collectivist countries described their beliefs and classroom practices was carried out. Through the constant comparative method, the two authors grouped the findings into categories of beliefs and practices which were then examined through the lens of the six dimensions of Hofstede’s model of cultural difference. Six categories of classroom practices, Pedagogy, Interaction, Student Role, Teacher Support, Differential Assessment, and Behavioral Management and three …
Species Specific Microcalcification In Reef Building Caribbean Corals In Ocean Acidification Conditions, Ashley M. Dungan
Species Specific Microcalcification In Reef Building Caribbean Corals In Ocean Acidification Conditions, Ashley M. Dungan
HCNSO Student Theses and Dissertations
Coral reefs are one of the most economically important ecosystems on the planet. Despite their great contribution to the world economy, anthropogenic influence via carbon dioxide emissions is leading to unprecedented changes with concerns about subsequent negative impacts on reefs. Surface ocean pH has dropped 0.1 units in the past century; in spite of this rapid shift in oceanic chemistry, it is unclear if individual species or life stages of Caribbean stony corals will be more sensitive to ocean acidification (OA). Examined is the relationship between CO2-induced seawater acidification, net calcification, photosynthesis, and respiration in three model Caribbean …
Intrinsic Challenges In Ancient Microbiome Reconstruction Using 16s Rrna Gene Amplification, Kirsten Ziesemer, Allison Mann, Krithivasan Sankaranarayanan, Hannes Schroeder, Andrew T. Ozga, Bernd W. Brandt, Egija Zaura, Andrea Waters-Rist, Menno Hoogland, Domingo C. Salazar-Garcia, Mark Aldenderfer, Camilla Speller, Jessica Hendy, Darlene A. Weston, Sandy J. Macdonald, Gavin H. Thomas, Matthew J. Collins, Cecil M. Lewis Jr., Corinne Hofman, Christina Warinner
Intrinsic Challenges In Ancient Microbiome Reconstruction Using 16s Rrna Gene Amplification, Kirsten Ziesemer, Allison Mann, Krithivasan Sankaranarayanan, Hannes Schroeder, Andrew T. Ozga, Bernd W. Brandt, Egija Zaura, Andrea Waters-Rist, Menno Hoogland, Domingo C. Salazar-Garcia, Mark Aldenderfer, Camilla Speller, Jessica Hendy, Darlene A. Weston, Sandy J. Macdonald, Gavin H. Thomas, Matthew J. Collins, Cecil M. Lewis Jr., Corinne Hofman, Christina Warinner
Biology Faculty Articles
To date, characterization of ancient oral (dental calculus) and gut (coprolite) microbiota has been primarily accomplished through a metataxonomic approach involving targeted amplification of one or more variable regions in the 16S rRNA gene. Specifically, the V3 region (E. coli341–534) of this gene has been suggested as an excellent candidate for ancient DNA amplification and microbial community reconstruction. However, in practice this metataxonomic approach often produces highly skewed taxonomic frequency data. In this study, we use non-targeted (shotgun metagenomics) sequencing methods to better understand skewed microbial profiles observed in four ancient dental calculus specimens previously analyzed by amplicon …
The Genome Russia Project: Closing The Largest Remaining Omission On The World Genome Map, T. K. Oleksyk, Vladimir Brukhin, Stephen J. O'Brien
The Genome Russia Project: Closing The Largest Remaining Omission On The World Genome Map, T. K. Oleksyk, Vladimir Brukhin, Stephen J. O'Brien
Biology Faculty Articles
We are witnessing the great era of genome exploration of the world, as genetic variation in people is being detailed across multiple varied world populations in an effort unprecedented since the first human genome sequence appeared in 2001. However, these efforts have yet to produce a comprehensive mapping of humankind, because important regions of modern human civilization remain unexplored. The Genome Russia Project promises to fill one of the largest gaps, the expansive regions across the Russian Federation, informing not just medical genomics of the territories, but also the migration settlements of historic and pre-historic Eurasian peoples.
Bacterial Community Dynamics In Marine Sponge Cinachyrella Kuekenthali Under Irradiance And Antibiotics, Nidhi Vijayan
Bacterial Community Dynamics In Marine Sponge Cinachyrella Kuekenthali Under Irradiance And Antibiotics, Nidhi Vijayan
HCNSO Student Theses and Dissertations
The Marine sponge Cinachyrella sp used in this study are commonly found in offshore South Florida and Caribbean waters and appeared to be resilient in closed system aquaculture. Marine sponges host diverse bacterial symbionts that are distinct compared to bacteria found in ambient seawater, however the roles of a large fraction of the bacterial community in marine sponges are unknown. Comparison of symbiotic to aposymbiotic (bacteria-free) sponges could provide information about interactions (metabolic and physiologic) between the bacteria and sponge. In this study, a single Cinachyrella kuekenthali individual was subsectioned into explants (N=240) in order to provide identical bacterial communities …