Digital Commons Network^™

Survival And Development Of Zebra Longwing, Heliconius Charithonia, On Native And Non-Native Passion Vines, Passiflora Sp., In South Florida, Cassidy Loucks, Celina Nelson-Jordan

Undergraduate Student Symposium

Zebra longwing butterflies (Heliconius charithonia) are distributed throughout Central and South America, and the southernmost regions of the United States, including Florida. These heliconian butterflies not only consume nectar, but are also known to feed on pollen, resulting in a longer lifespan than most butterflies. In their larval stage, H. charithonia feed exclusively on Passiflora spp, however preferred host species for H. charithonia eggs and overall larval performance across Passion vine species is not well documented. We examined these criteria of zebra longwings from egg to adulthood on two native passion vines to Florida, corky stem (Passiflora …

The Effect Of Fluorinated Substitution Of Meso Phenyl Porphyrins On Porphyrin Basicity, Harsh Chheda, Rushi Patel

Undergraduate Student Symposium

The purpose of this study was to determine the effects of fluorinated substitution of meso phenyl porphyrins on porphyrin basicity. The protonation of meso-Tetra(2,3,4-trifluoromethylphenyl) porphyrin (TF) and meso-Tetra(pentafluorophenyl) porphyrin (PF) will be studied through UV vis spectroscopy. Protonation is achieved by titration of each porphyrin in toluene with trifluoroacetic acid (TFA). The presence of a wavelength shift from the free base Soret to the protonated Soret will indicate the formation of a dication. The data from the UV spectra will be used to calculate the pK_a values associated with each porphyrin. Based on the results, the average pK_{a …}

The Halls Of Power, Janay Joseph, Graciel Quezada, Bianca Vucetich

Undergraduate Student Symposium

“The Halls of Power” is a documentary short film about the life and work of Elijah Manley, a young politician and activist in South Florida. The main story focuses on Elijah Manley’s life and political activism as the youngest person to ever run for the Florida State Legislature. The second storyline will follow other young activists and organizers within the community. The goal of the documentary is to inform the audience about this activist and demonstrate how one can get involved in politics. We hope to encourage the young demographic to be more engaged with their local politics. Because politics …

The Impact Of Post-Traumatic Stress On Symptom Presentation Of Women With Gulf War Illness, Nandan Shastry, Esha Sultana

Undergraduate Student Symposium

Gulf War Illness (GWI) is a multi-symptomatic disorder characterized by fatigue, muscle pain, cognitive problems, gastrointestinal issues, etc. affecting an estimated 30% of the ~750,000 returning military Veterans of the 1990–1991 Persian Gulf War. Female Veterans deployed to combat in this war report medical symptoms, involving cognition and respiratory troubles, at twice the rate compared to nondeployed female Veterans of the same era. The heterogeneity of GWI symptom presentation complicates diagnosis as well as the identification of effective treatments. This is exacerbated by the presence of comorbidities which may be alleviated by defining subgroups of the illness, such as an …

The Teacher, Amari Russell

Undergraduate Student Symposium

“The Teacher” is a short film about a professor who gets harassed by one of his former students. The film is directed by Amari Russell. Another crew member/actor includes Adam Deross.

The Universal Mass Function And Its Applicability To Organic Molecules, Gabrielle Biltgen-Morrissette

Undergraduate Student Symposium

The ability to predict the yield when synthesizing an organic molecule is a challenging issue in organic chemistry and a major obstacle when planning a multi-step organic synthesis. To choose the most time, money, and waste efficient way it will be beneficial to be able to predict the yield of the product. The Universal Mass Function (UMF) states that cosmic objects on all scales are in direct relation to each other. It shows that massive objects are much rarer than objects with lower masses. The following research aims to examine if the UMF theory is applicable to the field of …

Tree Canopy Biodiversity In An Urban Forestry Setting On Nova Southeastern University's Main Campus, Katja Kramers, Mackenzie Soligon, Amber Orr, James Puentes

Undergraduate Student Symposium

Nova Southeastern University's (NSU) 314-acre main campus has many lush green spaces comprised of native, landscape, and educational garden areas. Understanding the biodiversity of the canopy and the overall composition of native and invasive species is important for measuring ecological health of the urban ecosystem, and to guide responsible grounds management practices. As the environment and landscape at NSU develops, it is important to keep records of these environmental changes over time. The purpose of the research is to investigate the biodiversity of the campus' green spaces and document the native species on campus. 10 zones were randomly selected from …

Understanding The Tradeoff Between Atp Metabolism And Growth In E. Coli, Naziba Nuha, Julia Gumirov, Ariane Kalifa

Undergraduate Student Symposium

The inoculum effect (IE) is a phenomenon in which the minimal inhibitory concentration (MIC) of an antibiotic increases with the density of a bacterial population. IE has been observed for nearly all antibiotics, and has been shown to decrease antibiotic efficacy, prolong the recovery period and increase mortality rates. Despite its ubiquity in the clinic, a mechanism to explain IE for bacteria that expresses antibiotic resistant elements, such as β-lactamases, has yet to be identified. Interesting enough, recent work has shown that interactions between growth rate and metabolism, as determined through ATP production, affect IE for bacteria that lack a …

Using Social Media To Educate And Increase Awareness About The Harmful Effects Of Tobacco And Its Relationship With Covid-19, Rylee Azia Gardner, Tina Takla

Undergraduate Student Symposium

Tobacco use is the leading cause of deaths, diseases, and health care costs in our society. The Area Health Education Center (AHEC) Program at Nova Southeastern University launched a pilot social media campaign in June 2021 to encourage widespread distribution of information on the harmful effects of tobacco use across multiple social media platforms (Facebook, Instagram, Twitter, and LinkedIn). This initiative is student driven and faculty supported. Its goals are to increase social consciousness about the harmful effects of tobacco use through the widespread sharing of messaging and to motivate tobacco users to seek readily available cessation services provided through …

How The Molecular Vibrations Of Water May Prevent Skin Cancer, Ramson Munoz Morales, Jenna Handal

Undergraduate Student Symposium

Studies have shown that when DNA absorbs ultraviolet light it can become structurally damaged leading to a mutation and possibly skin cancer. However, exposing skin to sunlight rarely leads to a mutation. This indicates that after the ultraviolet light is absorbed by the DNA the energy from the light can either cause a structural change to the DNA (a mutation) or be dissipated in a way that does not damage the DNA. Absorption of ultraviolet light causes molecular vibrations in DNA and these vibrations are what likely leads to structural changes in the DNA. One way the structural changes could …

Molecular Biomarkers Of Ptsd: A Case For Measuring Psychotherapy Efficacy And Mtdna Cn Involvement, Ariana Ali, Aidan Kunju

Undergraduate Student Symposium

Post Traumatic Stress Disorder (PTSD) is a psychiatric disorder that occurs in individuals who witness or experience a traumatic event. Many PTSD treatments have a focus on direct alterations of emotion and thought responses due to trauma. Some of the leading psychotherapy treatments for ptsd include Cognitive Behavioral Therapy (CBT), Cognitive Processing Therapy (CPT), and Prolonged Exposure (PE). Eye Movement Desensitization and Realization (EMDR) Therapy has greater focus on altering memory storage in the brain. EMDR differs from other psychotherapies in that the reprocessing of trauma engages both hemispheres of the brain via bilateral stimulation. EMDR along with other psychotherapies …

Structural Identification Of Secondary Metabolites From Bacteria, Vijay Patel, Mark Sourial, Alana Bertrand

Undergraduate Student Symposium

Chronic misuse and abuse of antibiotics over the last century has led to an end of the golden age of antibiotics. However, we could turn to bacteria to find a solution to the current antibiotic resistance crisis. Bacteria are engaged in an endless arms race against each other, and humans can use the compounds bacteria produce as antibiotics. Our team isolated bacteria from local soil samples and aimed to identify and characterize these microbes. The two bacterial strains we were able to identify were Pseudomonas fulva and Plesiomonas shigelloides. P. fulva is an aerobic proteobacterium which produces secondary metabolites …

Covid-19 Vaccine Hesitancy In Pregnant Women From Marginalized Communities, Leona Markose

Undergraduate Student Symposium

Vaccination is known to be one of the most successful public health measures currently available that decreases numbers of mortality and morbidity of various diseases across the globe. Despite the success that has been found in controlling infectious disease through herd immunity from vaccinations, there is a rising problem especially prevalent among developed countries of a voluntarily under vaccinated population that do not believe these methods are safe or successful.

This delay in acceptance or refusal of vaccines despite availability of services is known as vaccine hesitancy. The COVID-19 pandemic sparked vaccine hesitancy throughout the United States, with an overabundance …

Elucidating The Function Of 3l1w, A Functionally Unknown Enzyme From Enterococcus Faecalis V583, Michael Egozi, Ethan Depry

Undergraduate Student Symposium

As of February 2022, there were 4057 protein structures with unknown functions in the Protein Data Bank. In all biological organisms there are a variety of enzymes that speed up key biochemical pathways. Despite having a known sequence and structure, many enzymes remain uncharacterized. One relevant enzyme identified is 3L1W, whose function has yet to be elucidated. The P.O.I. was isolated and imaged via Xray crystallography from the vancomycin resistant strain E. faecalis V583.

The purpose of this research was to provide an accurate characterization of 3L1W and its substrates, thus directing our approach of combining in silico and …

Evaluation Of Mdm2 Regulation On Aurkb Expression In Lung Cancer, Karen Kameo, Alexandra Carbone

Undergraduate Student Symposium

Objective. This study was conducted to analyze the molecular mechanism of the MDM2 regulation on AURKB in lung cancer cells. Background. Lung cancer is the leading cause of cancer death within the United States, with the 5-year survival rate at about 22%, indicating the need to develop different drugs and treatment strategies. This study focuses on understanding the mechanisms linking MDM2 and AURKB to develop new treatment strategies against lung cancer. Methods. The H446 cells were treated with RG7388 (2 μM), an MDM2 inhibitor, and Barasertib (50 nM), an AURKB inhibitor, including a combination of the two drugs for 24 …

Investigating Polymer Metal Chelation Via Atomic Absorption Spectroscopy, Meha Pandya, Agustin Olivares

Undergraduate Student Symposium

Heavy metal contaminants in water are known to disrupt ecological systems and cause detrimental biophysical and physiological effects in living systems. Prominent metallic environmental contaminants include lead, cadmium, and zinc. Removing heavy metal contaminants is essential to enhance water quality and reduce heavy metal toxicity. Chelating polymers that contain functional groups such as carboxyls and amines, offer an inexpensive and efficient way to remove metals from solution. The purpose of our study is to develop a protocol to quantify metal binding efficiency of various polymers via Atomic Absorption Spectroscopy (AAS). Alginate and Chitosan, two bio derived polymers with known chelating …

Metanalysis Of Genome Editing Using Crispr/Cas9 Technology Towards Hiv-1 Treatment, Nitya Alaparthy

Undergraduate Student Symposium

Genome editing, also called gene editing is a new branch of research that aims to explore means to modify genes of living organisms. Genome editing appears to have a promising future for researchers as it will further help to understand the gene function and develop ways to treat genetic or acquired diseases. Moreover, gene editing such as CRISPR/Cas9 technology can aid in manipulating the genome sequence, thereby, advancing treatments that will improve patients’ life span and quality of life. CRISPR/Cas9 technology supports three main mechanisms for genome editing, including plasmids-based methods, the direct intracellular distribution of the Cas9 mRNA, and …

A Preliminary Survey Of Dog-In-Prison Programs Across The Us, Isabella Defilippis, Janan Abushaban, Mariya Baig, Ariel Cherry, Naziha Mustafa, Trini Somasundaram, Ashesh Soni

Undergraduate Student Symposium

The molecular effects of canine-human bonding have not been well-studied, but both species are deeply driven to seek out prosocial interactions. We therefore expect to see quantitative changes in both physiological biomarkers (e.g. cell-level phenotypes linked to psychiatric disease) and animal behavior on both sides of the ancient (>15,000 year old) symbiosis between dogs and humans when dogs and people form interspecies dyads. A compelling context for studying this involves "dog-in-prison" programs, in which incarcerated humans help to train canines for future lives as companionship or service animals. Notably, both the incarcerated humans and orphaned/stray dogs within such programs …

A Computational Analysis Of Structural Analogs Of Colchicineas Potential Microtubule Polymerization Inhibitors, Isadora Rocha De Abreu, Allison Barkdull

Undergraduate Student Symposium

Microtubules are structures important for cell survival and cell function. They are responsible for the formation of the cytoskeleton and mitotic spindles, they aid in cell movement, intracellular transport, amongst other functions. Microtubules are composed of the tubulin protein made of alpha-beta dimer proteins. During polymerization tubulin proteins experience phases of dynamic instability, characterized by microtubule growth and shrinkage at varying rates. Microtubule growth can be inhibited by compounds such as colchicine, a known microtubule destabilizing agent, that binds to tubulin inhibiting microtubule polymerization. Colchicine has limited clinical use due to its toxicity and low solubility. In this study we …

An Episodic Study Of Hospital Charges For Hospital Admissions And Readmissions In 2017 In The United States For Degenerative Nerve Systems Disorders (Drg = 056), Esha Sultana

Undergraduate Student Symposium

Understanding hospital charges upon admission as well as readmissions is critical for value-based care. The extant literature focuses on patient-specific, disease-specific, and hospital-specific factors to explain the variance in hospital charges across the United States. Studies have mainly examined hospital charges upon admissions but there has been lack of empirical research on examining hospital charges upon readmissions. This has resulted in ambiguity in understanding the primary determinants of hospital charges upon readmissions. To address this gap, the federal agency AHRQ developed the HCUP hospital readmissions database that produces nationally representative information about hospital readmissions. This study utilizes the AHRQ 2017 …

Benefits Of Stem Mentorship: Introducing Microbiology Research To High School Students, Tassnime Sebaei, Katrina Maktaz, Varsha Chigurupati

Undergraduate Student Symposium

Bacteria Unearthed is an NSU initiative to bring together high school and college students to participate in Tiny Earth: an international consortia of researchers searching for novel microorganisms and antibiotics to combat antibiotic drug resistance. Our group developed a STEM mentor program as a catalyst for students to significantly increase academic performance. The program allows high schoolers to become part of the global research community. These high school students enrolled in their microbiology classes are supported by undergraduate students who have experience working with Tiny Earth. Through this experiential learning, it is expected that there will be improvements in …

Comparative Study Of Homocoupling Reactions To Create 2,2'-Bipyridine Adducts, Ethan Depry, Hoa Le

Undergraduate Student Symposium

The project focuses on investigating the synthesis of 2,2’-bipyridine adducts for potential use as ligands in novel organometallic ruthenium complexes. The bipyridine building blocks, based on their excellent bidentate ligands with complexing abilities, will be incorporated into ruthenium complexes in a collaborative project, increasing the photosensitivity of these complexes, and opening the doors for many applications, ranging from synthetic photovoltaics to anti-cancer activity.

The aim of this study was to optimize the preparation of the 2,2’-bipyridine adducts. Prior studies conducted in our lab have compared several homo and cross coupling reactions. It was determined that homocoupling offers a more efficient …

Effect Of β-Lactamase Expression And Varying Metabolism On The Inoculum Effect, Kielan Singh, Vijay Patel

Undergraduate Student Symposium

Infections with antibiotic resistant bacteria pose a substantial challenge to the field of medicine. Accordingly, it is of the utmost importance that we understand the mechanisms by which bacteria resist antibiotics towards the development of novel drugs and treatment paradigms. One mechanism by which a population of bacteria can resist an antibiotic is the inoculum effect (IE). During IE, the initial density of the population determines the efficacy of the antibiotic; highly dense populations of bacteria require more antibiotics to kill the population as compared to less dense populations. One mechanism that has been proposed to explain IE is the …

Caribbean Reef-Building Coral-Symbiodiniaceae Network: Identifying Symbioses Critical For System Stability In A Changing Climate, Mackenzie Magyar, Brady Estrada, Timothy Swain Ph.D.

Undergraduate Student Symposium

Coral reef bleaching events across the globe are increasing following intensifying marine heatwaves and higher ocean temperatures. The frequency and intensity of bleaching events are expected to rise over the next 100 years. An increase in coral mortality has been observed in the Caribbean between 1970-2012. Our objective is to produce a network of all Caribbean hermatypic coral-Symbiodiniaceae associations and analyze it in the context of increased temperatures and coral bleaching events. The available scientific literature was explored and inspected to evaluate whether these papers included relevant data for building the network, including the coral host, host depth, sample location, …

Deficits In Gait Biomechanics In Runners On A University Team: Implications For Preventative Sport Healthcare, W. Zachary Dean

Undergraduate Student Symposium

Sport healthcare providers in team-based settings conduct functional testing to identify injury risk. Results are used in team-based injury prevention programs. This process is different than care for a patient once they become injured. Gait analyses are a functional test for runners. Therefore, we aimed to describe the bilateral running biomechanics in a university cross country team with emphasis on variables linked to running-related injury (RRI). Twenty-seven male (n=10) and female (n=17) distance runners (age, 18-23yrs; height, 1.82±0.57m; mass, 58.4±6.8 kg) from a single university team participated in this descriptive study. Gait was assessed during a treadmill run at a …

Effect Of Periodontitis On Inducing Late Onset Alzheimer's Disease (Load) Pathology In Mice, Neha Gupta, Courtney Mai, Miguel Cuello

Undergraduate Student Symposium

Alzheimer’s disease is the most common form of dementia; affecting approximately 50 million people worldwide. Recent evidence indicates poor dental hygiene may accelerate Alzheimer’s degeneration. Experimenters used a mouse model of periodontitis induced by P. gingivalis and investigated the implications of poor dental health on Alzheimer’s Disease and neuroinflammation, as well as memory skills, in relation to aging, gender, and condition. All the mice in the model were the same age, and a total of 8 mice with Alzheimer’s Disease and 8 mice who had Alzheimer’s Disease with inflammation with an equivalent number of males and females were utilized. Experimenters …

Endoparasite Community Of Common Wood-Warblers (Family Parulidae) In Southeast Florida, Skylar Olsen

Undergraduate Student Symposium

Wood-warblers are small insectivorous passerine birds in the family Parulidae. They are among the first migratory birds in North America to begin their southward migration during the early autumn months and southeast Florida serves as a wintering ground. Although wood-warblers are common and widespread, knowledge of their endoparasite community composition is severely underrepresented in current literature. Carcasses were opportunistically collected from wildlife rescue centers in southeast Florida. The digestive tract of 90 birds representing 5 common wood-warbler species were examined for endoparasites: Common Yellowthroats (Geothlypis trichas), Ovenbirds (Seiurus aurocapilla), Black-and--White Warblers (Mniotilta varia), Palm Warblers (Setophaga palmarum), and American Redstarts …

Heavy Metal Contaminants In The Sciaenids From Lake Sabine, Katerina Sawickij

Undergraduate Student Symposium

Lake Sabine is a shallow, estuarine lake situated on the border between Texas and Louisiana with outflow to the Gulf of Mexico. The Louisiana side is a protected natural area while the Texas side is susceptible to pollution from several commercial industries as well as maintenance dredging. This study determined the concentrations of metal(loids) within commercially and economically important fish tissues and compared them intra- and inter-specifically using known diets and a range of sizes. Specimens were collected from Lake Sabine from April through October 2018 from four sciaenid fishes: Atlantic Croaker Micropogonias undulatus, Red Drum Sciaenops ocellatus, …

Induction Of Necroptosis In Mcf-7 Breast Cancer Cells Through Caspase-Independent Mechanisms Following Saha Treatment, Grace Waldron

Undergraduate Student Symposium

Currently, many anti-cancer drugs are known to induce programmed cell death (apoptosis) through activation of intrinsic or extrinsic pathways. However, many types of cancer cells have acquired resistance to apoptosis-mediated cell death. Therefore, simultaneous induction of other forms of cell death in addition to apoptosis of cancer cells is becoming an attractive strategy. For this purpose, we initially explored the role of XIAP in caspase-dependent and caspase-independent mechanisms of cell death. Our western blot data revealed that RIPK3 and MLKL levels were significantly elevated in MCF-7 cells following SAHA treatment. During induction of SAHA-induced cell death, the XIAP levels were …

How Non-Profit Organizations Can Create A Hybrid Social Media Strategy Using Paid And Organic Posts, Samantha Carl Selorio

Undergraduate Student Symposium

The emergence of social media has changed the methods and approaches for communication between health agencies and their community. As such, non-profit organizations must learn to use social media to better promote their mission. Organizations can engage and capture the attention of their online target audience via paid posts. Broward Regional Health Planning Council (BRHPC), a non-profit organization, provides an analysis of how integrating social media tools has increased its social media presence. BRHPC already had an online presence on the social media sites Facebook, Twitter, Instagram, and LinkedIn. To increase the number of people in BRHPC’s intended audience who …