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Next Generation Sequencing Methods For Coastal Zone Water Quality Monitoring, Catherine Iskrenko Aug 2018

Next Generation Sequencing Methods For Coastal Zone Water Quality Monitoring, Catherine Iskrenko

HCNSO Student Capstones

When analyzing the water quality of the coastal zone, culture-based techniques have been utilized most often to identify Fecal Indicator Bacteria in samples. Since the advent of the Sanger Method for DNA sequencing, other techniques have arisen that provide significantly more information on the microorganisms in sample, but they are still not the mainstream for water quality analysis. This capstone reviews and compares culture-based techniques, DNA sequencing, RNA sequencing, qPCR for biomarker, and 16S rDNA sequencing to highlight their merits and shortcomings for analyzing environmental water samples. The technique presented that provides the broadest range of information (including the identification …


Inclusion For A Student With Vision Impairment: “They Accept Me, Like, As In I Am There, But They Just Won’T Talk To Me.”, Jill L. Opie, Jane Southcott Aug 2018

Inclusion For A Student With Vision Impairment: “They Accept Me, Like, As In I Am There, But They Just Won’T Talk To Me.”, Jill L. Opie, Jane Southcott

The Qualitative Report

We explore the experiences of Nick, a secondary school student with vision impairment in an Australian mainstream school in this study, and we particularly focus on whether he perceived his education as inclusive. We have used Interpretative Phenomenological Analysis in this single individual case as this approach explores our participant’s understandings which may be revealed by close examination of mindful experiences. The “gem” spoken by Nick (pseudonym), our 16-year old participant, was “They accept me, like as in I am there, but they just won’t talk to me.” This statement summarises his sense of not belonging, of being other, and …


Medication Exposure Patterns In Primary Care Patients Prescribed Pharmacogenetically Actionable Opioids, Mitchell R. Knisely, Janet S. Carpenter, Marion E. Broome, Ann M. Holmes, Diane Von Ah, Todd Skaar, Claire Burke Draucker Aug 2018

Medication Exposure Patterns In Primary Care Patients Prescribed Pharmacogenetically Actionable Opioids, Mitchell R. Knisely, Janet S. Carpenter, Marion E. Broome, Ann M. Holmes, Diane Von Ah, Todd Skaar, Claire Burke Draucker

The Qualitative Report

Current approaches to assessing medication exposure fail to capture the complexity of the phenomenon and the context in which it occurs. This study’s purpose was to develop a typology of subgroups of patients who share common patterns of medication exposure. To create the typology, we used an exemplar sample of 30 patients in a large public healthcare system who had been prescribed the pharmacogenetically actionable opioids codeine or tramadol. Data related to medication exposure were drawn from large data repositories. Using a person-oriented qualitative approach, eight subgroups of patients who shared common patterns of medication exposure were identified. The subgroups …


From Contemplation To Action: Mechanisms Of Change In The Mentoring Academy, Linda S. Behar-Horenstein, Huibin Zhang Aug 2018

From Contemplation To Action: Mechanisms Of Change In The Mentoring Academy, Linda S. Behar-Horenstein, Huibin Zhang

The Qualitative Report

Mentoring is fundamental to the professional development of research scientists in academic health centers (AHC). Qualified mentors can support the development of competencies considered most significant in training research scientists. Yet AHC faculty may have little preparation in and knowledge of how to mentor. Emerging AHC mentor academies provide educational environments whereby faculty can learn the art and practice of mentoring. However, little is known about their effectiveness. Using the Transtheoretical Change Model (TTM), this study explored how 23 mentors used newly learned information to change their communication styles and develop shared expectations with mentees. Based on an inductive analysis …


Microbial Ecology Of South Florida Surface Waters: Examining The Potential For Anthropogenic Influences, Chase P. Donnelly Aug 2018

Microbial Ecology Of South Florida Surface Waters: Examining The Potential For Anthropogenic Influences, Chase P. Donnelly

HCNSO Student Theses and Dissertations

South Florida contains one of the largest subtropical wetlands in the world, and yet not much is known about the microbes that live in these surface waters. These microbes play an important role in chemical cycling and maintaining good water quality for both human and ecosystem health. The hydrology of Florida’s surface waters is tightly regulated with the use of canal and levee systems run by the US Army Corps of Engineers and The South Florida Water Management District. These canals run through the Everglades, agriculture, and urban environments to control water levels in Lake Okeechobee, the Water Conservation Areas, …


Using Expert Interviews Within Modes In Online And Offline Spaces To Extend Comprehensive Literature Review Processes, Alana Morris, Anthony J. Onwuegbuzie, Hannah R. Gerber Aug 2018

Using Expert Interviews Within Modes In Online And Offline Spaces To Extend Comprehensive Literature Review Processes, Alana Morris, Anthony J. Onwuegbuzie, Hannah R. Gerber

The Qualitative Report

In this article, we explore a 7-step process for conducting a comprehensive literature review (CLR). Specifically, after describing each of the steps, we explain the importance of expanding the search beyond traditional databases through 5 multimodal tasks that Onwuegbuzie and Frels (2016) refer to as MODES (Media, Observations, Documents, Experts, and Secondary Data), which can be undertaken separately, or which may interact with each other. Then, we highlight and provide an exemplar process for the Experts task, which motivates researchers to identify, to seek out, and to interview 1 or more experts associated with their research question(s). Furthermore, we illustrate …


Discovering Meaning-Making In A Child Custody Context: The Combined Benefits Of Using Van Manen's Phenomenology And A Critical Lens, Beth Archer-Kuhn Aug 2018

Discovering Meaning-Making In A Child Custody Context: The Combined Benefits Of Using Van Manen's Phenomenology And A Critical Lens, Beth Archer-Kuhn

The Qualitative Report

Phenomenology as a methodology reveals lived experience (van Manen, 2017); as such, it is an excellent methodology to help us understand the parent perceptions and experiences of child custody decision-making. Qualitative researchers have explored the ways in which lived experience is influenced by issues of culture and gender (Kall & Zeiler, 2014). In this paper, I explain how the key tenets of phenomenology, epoché and reduction, revealed the importance of custody status for participants, and also discuss how differences in gender was rendered invisible in the initial analysis. The flexible nature of phenomenology allowed me to use a critical lens …


Interpersonal Hardiness As A Critical Contributing Factor To Persistence Among International Women In Doctoral Programs: A Trioethnographic Study, Eraldine S. Williams-Shakespeare 2153760, Joyce E. Bronteng, Adhwaa Alahmari Aug 2018

Interpersonal Hardiness As A Critical Contributing Factor To Persistence Among International Women In Doctoral Programs: A Trioethnographic Study, Eraldine S. Williams-Shakespeare 2153760, Joyce E. Bronteng, Adhwaa Alahmari

The Qualitative Report

Women in PhD programs, in particular minority and international women, are especially at risk for drop-out (Castro, Garcia, Cavazos, & Castro, 2011; Haynes et al., 2012). This initial part of a longitudinal trioethnography captures the experiences of three international women in a doctoral program, highlighting the challenges, support systems, and coping mechanisms they engage with in the process of completing their degrees. Discoveries include the identification of “Interpersonal Hardiness” as the potential vehicle which could ensure our success.


Researching The Mechanisms Of Gossip In Organizations: From Fly On The Wall To Fly In The Soup, Dominique J. Darmon Jul 2018

Researching The Mechanisms Of Gossip In Organizations: From Fly On The Wall To Fly In The Soup, Dominique J. Darmon

The Qualitative Report

In this paper, I explored how to research a sensitive topic such as gossip in organizations and used a narrative approach to illustrate the methodological and ethical issues that come up when considering a variety of research methods. I first attempted to conduct an ethnographic research on a project group from a Dutch university undergoing a major change. At the very beginning of the project, as a participant observer, I struggled to remain an outsider, or a “fly on the wall.” But as issues of power came into play and access became increasingly problematic, I moved towards the role of …


Parasitic Indicators Of Foraging Strategies In Wading Birds, Sarah Gumbleton Jul 2018

Parasitic Indicators Of Foraging Strategies In Wading Birds, Sarah Gumbleton

HCNSO Student Theses and Dissertations

Feeding ecology and trophic interactions of six species of wading birds were explored through a combined analysis of stable isotope profiles and endoparasite communities. Stable isotopes broadly characterize the feeding preferences and geographic information, while parasite communities reflect long-term trends in feeding ecology. Deceased birds were obtained from four South Florida wildlife rehabilitation organizations. Of the 81 birds dissected, 73 contained parasites. Parasites were predominately found within the gastrointestinal tract. Host and range extensions were noted for several parasite taxa. Bird host species had a significant effect on the parasite community (P=0.001) while wildlife center location and maturity status did …


Building Community In An Online Graduate Program: Exploring The Role Of An In-Person Orientation, Sharla Berry Jul 2018

Building Community In An Online Graduate Program: Exploring The Role Of An In-Person Orientation, Sharla Berry

The Qualitative Report

Orientations help students transition into academic programs. At orientation, students learn how to navigate the university, and access support services, and build rapport with faculty, staff and other students. Few studies have explored the role of orientation in online programs. This qualitative case study explores the ways in which a three-day, in-person orientation impacted students’ sense of community in one online graduate program. Findings indicate that providing online students with unstructured, in-person opportunities for interaction helped them develop a sense of community.


On The Same Page: A Formal Process For Training Multiple Interviewers, Carolyn Sattin-Bajaj Jul 2018

On The Same Page: A Formal Process For Training Multiple Interviewers, Carolyn Sattin-Bajaj

The Qualitative Report

The increased utilization of qualitative methodologies as part of mixed-method health and social science research has highlighted the need for training procedures for every stage of qualitative data collection and analysis. Yet, few group training models exist for collecting reliable, valid qualitative interview data. This article presents a multi-stage, collaborative interview training process for a large team of research assistants. The training program combines insights and techniques used in both structured and semi-structured interviewing. It also includes ongoing instruction and feedback prior to and during data collection in an effort to ensure consistency and reliability. In the article, I describe …


Applications Of Click Chemistry In The Development Of Hiv Protease Inhibitors, Mukesh Madan Mudgal, Nagaraju Birudukota, Mayur A. Doke Jul 2018

Applications Of Click Chemistry In The Development Of Hiv Protease Inhibitors, Mukesh Madan Mudgal, Nagaraju Birudukota, Mayur A. Doke

Chemistry and Physics Faculty Articles

Acquired Immunodeficiency Syndrome (AIDS) has been devastating for millions of people around the world. Inhibition of the human immunodeficiency virus (HIV) protease is among the most important approaches for the therapeutic intervention in HIV infection. Since the discovery of the HIV-1 protease, this enzyme has been considered as a key target for the inhibition of viral replication. A large body of research has been done to develop an effective HIV-1 protease inhibitor. There are to date 10 HIV-1 protease inhibitor drugs approved by the Food and Drug Administration (FDA) that have improved the survival and quality of life of HIV …


Visual Inspection Of Sequential Data: A Research Instrument For Qualitative Data Analysis, Alaa A. Aldahdouh Jul 2018

Visual Inspection Of Sequential Data: A Research Instrument For Qualitative Data Analysis, Alaa A. Aldahdouh

The Qualitative Report

Sequence analysis has been widely used to investigate the patterns of similarities and differences of sequential data in biology and sociology. However, the debate on the usage of sequence analysis in social sciences has not been settled yet. Among a long list, sequence analysis methods have been criticized for ignoring the qualitative information behind the sequences. This paper presents a new instrument for inspecting sequential data visually in qualitative studies. The method includes building a hierarchical tree of relations among the categories which is then used to recode the categories systematically. The recoding process is meant to give meaning to …


Elderly Patients’ Perception Of Pain Management After Open And Reduction Internal Fixation Surgery, Sharon M. Whyte-Daley Jul 2018

Elderly Patients’ Perception Of Pain Management After Open And Reduction Internal Fixation Surgery, Sharon M. Whyte-Daley

The Qualitative Report

Little is known about pain and pain management in older adults who experience open reduction and internal fixation (ORIF) surgery. This qualitative descriptive phenomenological study explored two research questions: (a) What are the perceptions of pain and pain management in patients between 65 and 75 years of age, 48 hours after ORIF surgery in a community hospital? (b) What are the perceptions of adaptation after ORIF? A pilot study included four patients in two units of a Southern California hospital, followed by open ended, semi-structured interviews with 10 participants. Four themes emerged: (a) elderly patients experience different patterns of pain …


Analytical “Bake-Off” Of Whole Genome Sequencing Quality For The Genome Russia Project Using A Small Cohort For Autoimmune Hepatitis, Daria V. Zhernakova, Sergei Kliver, Nikolay Cherkasov, Gaik Tamazian, Mikhail Rotkevich, Ksenia Krasheninnikova, Igor Evsyukov, Sviatoslav Sidorov, Pavel Dobrynin, Andrey A. Yurchenko, Valentin Shimansky, Irina V. Shcherbakova, Andrey S. Glotov, David L. Valle, Minzhong Tang, Emilia Shin, Kathleen B. Schwarz, Stephen James O'Brien Jul 2018

Analytical “Bake-Off” Of Whole Genome Sequencing Quality For The Genome Russia Project Using A Small Cohort For Autoimmune Hepatitis, Daria V. Zhernakova, Sergei Kliver, Nikolay Cherkasov, Gaik Tamazian, Mikhail Rotkevich, Ksenia Krasheninnikova, Igor Evsyukov, Sviatoslav Sidorov, Pavel Dobrynin, Andrey A. Yurchenko, Valentin Shimansky, Irina V. Shcherbakova, Andrey S. Glotov, David L. Valle, Minzhong Tang, Emilia Shin, Kathleen B. Schwarz, Stephen James O'Brien

Biology Faculty Articles

A comparative analysis of whole genome sequencing (WGS) and genotype calling was initiated for ten human genome samples sequenced by St. Petersburg State University Peterhof Sequencing Center and by three commercial sequencing centers outside of Russia. The sequence quality, efficiency of DNA variant and genotype calling were compared with each other and with DNA microarrays for each of ten study subjects. We assessed calling of SNPs, indels, copy number variation, and the speed of WGS throughput promised. Twenty separate QC analyses showed high similarities among the sequence quality and called genotypes. The ten genomes tested by the centers included eight …


Spatiotemporal Change In The Benthic Community Of Southeast Florida, Nicholas P. Jones Jul 2018

Spatiotemporal Change In The Benthic Community Of Southeast Florida, Nicholas P. Jones

HCNSO Student Theses and Dissertations

High-latitude reefs have been postulated as refugia, centers for resilience or the first areas to undergo re-organization under climate change. The Southeast Florida Reef Tract (SEFRT) is a high-latitude reef system (>25 °N) running parallel to the highly urbanized coastline of southeast Florida. With a benthic community comprised of a mixture of coral reef associated assemblages, the SEFRT is towards the northern limit of stony coral cover due to temperature constraints. This study analyzed spatial variations in benthic cover, spatiotemporal changes in the benthic community and the impact of spatial and temporal fluctuations in temperature on benthic cover on …


A Motherwork Challenge To Dominant Discourse: A Review Of Immigration, Motherhood, And Parental Involvement: Narratives Of Communal Agency In The Face Of Power Asymmetry, Katie D. Scott Jul 2018

A Motherwork Challenge To Dominant Discourse: A Review Of Immigration, Motherhood, And Parental Involvement: Narratives Of Communal Agency In The Face Of Power Asymmetry, Katie D. Scott

The Qualitative Report

In Lilian Cibils dissertation-turned-book, Immigration, Motherhood and Parental Involvement: Narratives of Communal Agency in the Face of Power Asymmetry (2017), the stories of seven Mexican immigrant mothers provide insight into what motherhood looks like outside the mainstream ideology of parental involvement. Using a critical feminist lens, Cibils employs the concept of motherwork as an alternative to a cultural deficit approach for understanding Mexican immigrant motherhood.


Understanding The Perceived Effectiveness Of Applying The Visitor Experience And Resource Protection (Verp) Framework For Recreation Planning: A Multi-Case Study In U.S. National Parks, Jessica Fefer, Sandra M. De Urioste-Stone, John Daigle, Linda Silka Jul 2018

Understanding The Perceived Effectiveness Of Applying The Visitor Experience And Resource Protection (Verp) Framework For Recreation Planning: A Multi-Case Study In U.S. National Parks, Jessica Fefer, Sandra M. De Urioste-Stone, John Daigle, Linda Silka

The Qualitative Report

The Visitor Experience and Resource Protection (VERP) framework is a planning framework developed by the U.S. National Park Service (NPS) to help guide visitor use planning and decision-making in U.S. national parks. The research reported here highlights the perceptions of park practitioners about major successes and challenges associated with visitor management and recreation planning using the VERP framework. We used a qualitative multiple case study design to explore three (3) national parks that have applied the framework. We conducted 16 semi-structured interviews with park managers, park planners, and recreation scientists, and used thematic coding to categorize the data to capture …


Effects Of Web-Based Instruction And Patient Preferences On Patient-Reported Outcomes And Learning For Women With Advanced Ovarian Cancer: A Randomized Controlled Trial, Sue V. Petzel, Rachel Isaksson Vogel, Julie Cragg, Molly Mcclellan, Daniel Chan, Julie Jacko, François Sainfort, Melissa A. Geller Jul 2018

Effects Of Web-Based Instruction And Patient Preferences On Patient-Reported Outcomes And Learning For Women With Advanced Ovarian Cancer: A Randomized Controlled Trial, Sue V. Petzel, Rachel Isaksson Vogel, Julie Cragg, Molly Mcclellan, Daniel Chan, Julie Jacko, François Sainfort, Melissa A. Geller

HCBE Faculty Articles

A randomized controlled trial was conducted of a web-based intervention to improve advanced care planning in women with ovarian cancer. A secondary analysis of 35 randomized women focused on changes in distress and knowledge about ovarian cancer through distress monitoring and information tailored to patients' cognitive coping style (monitoring, blunting). Pre-/postresults indicated the Intervention group demonstrated lower distress (p = 0.06); blunting was associated with lower depression (p = 0.04); knowledge in both groups was unchanged. Women in the Intervention vs. Control group reported their family was less likely to be upset by cancer information (p = 0.0004). This intervention …


Being Māori And Pākehā: Methodology And Method In Exploring Cultural Hybridity, Niki Grennell-Hawke (Ngai Tahu, Ngai Mutunga), Keith Tudor Dr Jul 2018

Being Māori And Pākehā: Methodology And Method In Exploring Cultural Hybridity, Niki Grennell-Hawke (Ngai Tahu, Ngai Mutunga), Keith Tudor Dr

The Qualitative Report

This article addresses the first author’s experience of identifying as both Māori and Pākehā in Aotearoa New Zealand. Based on her own research using both kaupapa research theory and heuristic research method, and supervised by the second author, the article describes her negotiation of the experience of being a hybrid cultural subject and object, of belonging and not belonging. The article extends the practice and understanding of cross-cultural research on a number of levels: the intrapsychic (i.e., within the principal investigator herself), the interpersonal (i.e., between the researcher and supervisor), and the methodological (i.e., between an indigenous and a Western …


Perceived Stressors Of Hospitalized Patients’ Family In Cardiac Care Unites: A Qualitative Content Analysis, Parand Pourghane, Maryam Rajab Pour Nikfam, Abbas Ebadi Jul 2018

Perceived Stressors Of Hospitalized Patients’ Family In Cardiac Care Unites: A Qualitative Content Analysis, Parand Pourghane, Maryam Rajab Pour Nikfam, Abbas Ebadi

The Qualitative Report

The present study attempts to justify the factors inducing stress in the families of patients hospitalized in cardiac intensive care units in the east of Guilan province in Iran. The study aims at gaining an accurate understanding on these stressors for appropriate planning directed at removing or decreasing these tension-inducing components. The present study is a qualitative study based upon a conventional content analysis approach. The study population was selected through purposive sampling (28 family members of cardiac patients), and the data were gathered through semi-structured interviews. Data analysis was performed as per the stages recommended by Graneheim and Lundman …


Ethical And Methodological Issues Resulting From Recording Lapses In Qualitative Research, Jason D. Reynolds (Taewon Choi), Minsun Lee Jul 2018

Ethical And Methodological Issues Resulting From Recording Lapses In Qualitative Research, Jason D. Reynolds (Taewon Choi), Minsun Lee

The Qualitative Report

Technological difficulties, such as recording lapses and dropped calls, during interviews for qualitative research lead to important ethical and methodological considerations. Using case examples from our own experiences with recording lapses, we walk through some of the questions to consider, including relational ethics and how changes in the data affect the validity of our findings. We argue that how ethical and methodological issues raised by recording lapses are to be handled largely depends on the paradigm in which each study is situated, ranging from postpositivist to constructivist to critical theory. However, we recommend that: (a) participants should be informed about …


Com Outlook Summer-Fall 2018, College Of Osteopathic Medicine Jul 2018

Com Outlook Summer-Fall 2018, College Of Osteopathic Medicine

COM Outlook

No abstract provided.


Perspective Volume 6: Number 2, Summer-Fall 2018, College Of Health Care Sciences Jul 2018

Perspective Volume 6: Number 2, Summer-Fall 2018, College Of Health Care Sciences

Perspectives

No abstract provided.


Infuse!_Volume 2_Number 1_2018, College Of Nursing Jul 2018

Infuse!_Volume 2_Number 1_2018, College Of Nursing

Infuse! College of Nursing Magazine

No abstract provided.


July 2018 Student Handbook, Nova Southeastern University Jul 2018

July 2018 Student Handbook, Nova Southeastern University

NSU Student Handbooks

No abstract provided.


Nsu Sharks Rx-Summer, 2018, Nova Southeastern University Jul 2018

Nsu Sharks Rx-Summer, 2018, Nova Southeastern University

NSU Sharks Rx

No abstract provided.


Southeast Florida Large (>2 Meter) Diseased Coral Colony Intervention Summary Report, Brian K. Walker, Alysha Brunelle Jun 2018

Southeast Florida Large (>2 Meter) Diseased Coral Colony Intervention Summary Report, Brian K. Walker, Alysha Brunelle

Marine & Environmental Sciences Faculty Reports

No abstract provided.


The Roles We Played: Exploring Intimacy In Research, Kathleen M. Alley Jun 2018

The Roles We Played: Exploring Intimacy In Research, Kathleen M. Alley

The Qualitative Report

Intimate relationships can serve as catalysts impelling us to deeply interact with others, and, consequently helping us to develop a greater understanding of ourselves, those with whom we come into contact, and the wider world. This manuscript describes the challenges and constraints I faced when engaged in qualitative research with an intimate other. I borrow from Dr. Carolyn Ellis’ (2007) concept of relational ethics, which requires researchers to: (a) act from their hearts and minds, (b) acknowledge interpersonal bonds to others, and (c) take responsibility for actions and their consequences. Power is a part of intimate relationships, so exploring and …