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Relation Between Fine And Gross Motor Skills And Observation Of Sociocultural Factors Across The First Year Of Infancy, Erika Garcia Mora

LSU Doctoral Dissertations

The development of independent arms-free sitting, which frees an infant’s arms to explore objects in their environment (Soska & Adolph, 2014) is observed to propagate an increase in the frequency of manual and manipulatory actions (Marcinowski, et al., 2019; Mlincek, et. al., 2022). Active object exploration provides the infant with more sensory information of the fundamental characteristics of objects in their environment. Thus, this dissertation examined the quality and quantity of object exploration across seated postures.

Additionally, the literature claiming that infants raised in low-income households having fewer resources for effective development is being countered by considering a more holistic …

Using Machine Learning To Measure Changes In Immunity Due To Climate Change In The Fall Armyworm, Spodoptera Frugiperda, Nathaniel T. Haulk

LSU Master's Theses

Object detection is a recent development that allows for the detection of multiple objects in photographs. Numerous machine learning algorithms have been developed for object detection. Here we sought to determine which algorithm performed best when relating to an organism's health and immunological response. The fall armyworm (Spodoptera frugiperda) is a major agricultural pest causing millions of dollars in damage to farmlands across the globe. With their recent spread into Africa and Asia, the need for understanding how best to control population outbreaks via biocontrol has increased. While viruses are a major biocontrol agent for controlling insect pests, we …

Shifting Taxonomic And Functional Community Composition Of Rivers Under Land Use Change, Kai Chen, Stephen R. Midway, Brandon K. Peoples, Beixin Wang, Julian D. Olden

Faculty Publications

Land use intensification has led to conspicuous changes in plant and animal communities across the world. Shifts in trait-based functional composition have recently been hypothesized to manifest at lower levels of environmental change when compared to species-based taxonomic composition; however, little is known about the commonalities in these responses across taxonomic groups and geographic regions. We investigated this hypothesis by testing for taxonomic and geographic similarities in the composition of riverine fish and insect communities across gradients of land use in major hydrological regions of the conterminous United States. We analyzed an extensive data set representing 556 species and 33 …

The Future Of Cyanobacteria Toxicity In Estuaries Undergoing Pulsed Nutrient Inputs: A Case Study From Coastal Louisiana, Sibel Bargu, Matthew Hiatt, Kanchan Maiti, Paul Miller, John R. White

Faculty Publications

Harmful cyanobacteria blooms (cyanoHABs) are a global phenomenon, especially in calm, warm, and nutrient-rich freshwater and estuarine systems. These blooms can produce various potent toxins responsible for animal poisoning and human health problems. Nutrient-rich freshwater pulsed into estuaries affects turbidity, water temperature, salinity, and nutrient concentrations and ratios at irregular intervals, creating a highly dynamic habitat. However, the underlying processes that lead to the selective development of cyanoHABs for certain species and the fate of their toxins are still uncertain. This paper draws upon the rich body of research available for one such system, the Lake Pontchartrain Estuary, Louisiana, to …

Limnological Response Of Las Curias Reservoir, San Juan, Puerto Rico: Successful Management Of The Invasive Aquatic Fern, Salvinia Molesta, Xavier A. García-López, Jorge R. Ortiz-Zayas, Rodrigo Díaz, Aurelio Castro-Jiménez, Charles F. Wahl

Faculty Publications

The anthropogenic deterioration of aquatic ecosystems affects water resources due to agricultural malpractices, pollution from domestic septic tanks, recreational activities, and poor watershed management, among other factors. This study examines the management of Las Curias Reservoir, San Juan, Puerto Rico, after the 2016 arrival of the invasive aquatic fern Salvinia molesta. In September 2019, a community-led initiative introduced the Cyrtobagous salviniae weevil, an effective biological control agent for S. molesta, and commenced a mechanical removal campaign using an aquatic harvester. Limnological sampling (September 2019 to September 2022) and drone flights were employed to measure physicochemical and floating plant cover changes, …

A Phoenix Glimmers Within The Ashes: Generalized Defensive Traits Suggest Hope For Plants Under Attack By Invasive Pests, Todd D. Johnson, Justin G.A. Whitehill

Faculty Publications

No abstract provided.

Effect Of Type Of Mulch On Microbial Food Safety Risk On Cucumbers Irrigated With Contaminated Water, Juan Moreira, Kevin Mccarter, Julysa Abril Benitez, Kathryn Fontenot, Joan M. King, Achyut Adhikari

Faculty Publications

Mulches are used to block light and retain soil moisture which may affect the survival of bacterial pathogens on soil. This study examined the effectiveness of different types of mulches to minimize microbial risk from contaminated water used for irrigation of cucumbers. A production bed of 120 ft2 with 18 beds (30 ft long) covered with five different types of mulch (paper, paper with fertilizer incorporated (PF), maize-based mulch, biodegradable plastic covering, and conventional plastic) including three beds with no cover was planted with Dasher 2 Variety cucumber. Soil samples from each bed were collected for the first five weeks …

Filamentous Pathogen Effectors Enter Plant Cells Via Endocytosis, Haixia Wang, Ely Oliveira-Garcia, Petra C. Boevink, Nicholas J. Talbot, Paul R.J. Birch, Barbara Valent

Faculty Publications

Recent findings demonstrate that cytoplasmic effectors from fungal and oomycete pathogens enter plant cells via clathrin-mediated endocytosis (CME). This raises several questions: Does effector secretion pathway facilitate host uptake? How is CME triggered in host cells? How are the effectors released from endosomal compartments to reach diverse subcellular destinations?

A Membrane Protein Of The Rice Pathogen Burkholderia Glumae Required For Oxalic Acid Secretion And Quorum Sensing, Asif Iqbal, George Nwokocha, Vijay Tiwari, Inderjit K. Barphagha, Anne Grove, Jong Hyun Ham, William T. Doerrler

Faculty Publications

Bacterial panicle blight is caused by Burkholderia glumae and results in damage to rice crops worldwide. Virulence of B. glumae requires quorum sensing (QS)-dependent synthesis and export of toxoflavin, responsible for much of the damage to rice. The DedA family is a conserved membrane protein family found in all bacterial species. B. glumae possesses a member of the DedA family, named DbcA, which we previously showed is required for toxoflavin secretion and virulence in a rice model of infection. B. glumae secretes oxalic acid as a “common good” in a QS-dependent manner to combat toxic alkalinization of the growth medium …

Effects Of Ingested Essential Oils And Propolis Extracts On Honey Bee (Hymenoptera: Apidae) Health And Gut Microbiota, Allyson Martin Ewert, Michael Simone-Finstrom, Quentin Read, Claudia Husseneder, Vincent Ricigliano

Faculty Publications

Managed honey bee (Hymenoptera: Apidae: Apis mellifera Linnaeus) hives require frequent human inputs to maintain colony health and productivity. A variety of plant natural products (PNPs) are delivered via feeding to control diseases and reduce the use of synthetic chemical treatments. However, despite their prevalent use in beekeeping, there is limited information regarding the impact of ingested PNPs on bee health. Here, we tested the effects of different essential oils and propolis extracts on honey bee life span, nutrient assimilation, xenobiotic detoxification, and gut microbiota abundance. Brazilian propolis extract lengthened worker life span, while the other PNPs (Louisiana propolis extract, …

Weight And Protozoa Number But Not Bacteria Diversity Are Associated With Successful Pair Formation Of Dealates In The Formosan Subterranean Termite, Coptotermes Formosanus, Junyan Chen, Garima Setia, Li Hsiang Lin, Qian Sun, Claudia Husseneder

Faculty Publications

New colonies of Formosan subterranean termites are founded by monogamous pairs. During swarming season, alates (winged reproductives) leave their parental colony. After swarming, they drop to the ground, shed their wings, and male and female dealates find suitable nesting sites where they mate and become kings and queens of new colonies. The first generation of offspring is entirely dependent on the nutritional resources of the founder pair consisting of the fat and protein reserves of the dealates and their microbiota, which include the cellulose-digesting protozoa and diverse bacteria. Since termite kings and queens can live for decades, mate for life …

Minimal Shift Of Eastern Wild Turkey Nesting Phenology Associated With Projected Climate Change, Wesley W. Boone, Christopher E. Moorman, Adam J. Terando, David J. Moscicki, Bret A. Collier, Michael J. Chamberlain, Krishna Pacifici

Faculty Publications

Climate change may induce mismatches between wildlife reproductive phenology and temporal occurrence of resources necessary for reproductive success. Verifying and elucidating the causal mechanisms behind potential mismatches requires large-scale, longer-duration data. We used eastern wild turkey (Meleagris gallopavo silvestris) nesting data collected across the southeastern U.S. over eight years to investigate potential climatic drivers of variation in nest initiation dates. We investigated climactic relationships with two datasets, one inclusive of successful and unsuccessful nests (full dataset) and another of just successful nests (successfully hatched dataset), to determine whether successfully hatched nests responded differently to weather changes than all nests did. …

Community-Wide Correlations Between Species Richness, Abundance And Population Genomic Diversity In A Freshwater Biodiversity Hotspot, Jamie R. Bucholz, Garrett W. Hopper, Irene Sánchez González, Taylor E. Kelley, Colin R. Jackson, Ryan C. Garrick, Carla L. Atkinson, Jeffrey D. Lozier

Faculty Publications

Understanding patterns of diversity across macro (e.g. species-level) and micro (e.g. molecular-level) scales can shed light on community function and stability by elucidating the abiotic and biotic drivers of diversity within ecological communities. We examined the relationships among taxonomic and genetic metrics of diversity in freshwater mussels (Bivalvia: Unionidae), an ecologically important and species-rich group in the southeastern United States. Using quantitative community surveys and reduced-representation genome sequencing across 22 sites in seven rivers and two river basins, we surveyed 68 mussel species and sequenced 23 of these species to characterize intrapopulation genetic variation. We tested for the presence of …

Labeling Effects Of Initial Juvenile Justice System Processing Decision On Youth Interpersonal Ties*, Zachary R. Rowan, Adam Fine, Laurence Steinberg, Paul J. Frick, Elizabeth Cauffman

Faculty Publications

The juvenile justice system can process youth in myriad ways. Youth who are formally processed, relative to being informally processed, may experience more public and harsh sanctions that label youth more negatively as “deviant.” Drawing on labeling theory, the current study evaluates the relative effect of formal justice system processing on the interpersonal dynamics of youth peer networks. Using data from the Crossroads Study, a multisite longitudinal sample of first-time adolescent offenders, the current study applies augmented inverse probability weighting and generalized mixed-effects models to estimate the effects of formal processing on friendship selection processes of homophily and withdrawal and …

Adipocyte Stat5 (Signal Transducer And Activator Of Transcription 5) Is Not Required For Glucocorticoid-Induced Metabolic Dysfunction, Innocence Harvey, Allison J. Richard, Tamra M. Mendoza, Jacqueline M. Stephens

Division of Basic Science Faculty Publications

Excess glucocorticoid (GC) signaling in adipose tissue is a key driver of insulin resistance and hepatic steatosis, but underlying mechanisms have not been fully elucidated. Signal transducer and activator of transcription 5 (STAT5) signaling in adipocytes has also been implicated in the progression of similar metabolic disturbances. Although STAT5 has been shown to interact with the glucocorticoid receptor (GR) in many cell types including adipocytes, the relevance of the STAT5/GR complex has not been investigated in adipocytes. Adult male and female adipocyte-specific STAT5 knockout (STAT5AKO) and floxed mice were given corticosterone (CORT) or vehicle in their drinking water for 1 …

Omics-Driven Investigation Of The Biology Underlying Intrinsic Submaximal Working Capacity And Its Trainability, Monalisa Hota, Jacob L. Barber, Jonathan J. Ruiz-Ramie, Charles S. Schwartz, Do Thuy Uyen Ha Lam, Prashant Rao, Michael Y. Mi, Daniel H. Katz, Jeremy M. Robbins, Clary B. Clish, Robert E. Gerszten, Mark A. Sarzynski, Sujoy Ghosh, Claude Bouchard

Division of Basic Science Faculty Publications

Submaximal exercise capacity is an indicator of cardiorespiratory fitness with clinical and public health implications. Submaximal exercise capacity and its response to exercise programs are characterized by heritability levels of about 40%. Using physical working capacity (power output) at a heart rate of 150 beats/min (PWC150) as an indicator of submaximal exercise capacity in subjects of the HERITAGE Family Study, we have undertaken multi-omics and in silico explorations of the underlying biology of PWC150 and its response to 20 wk of endurance training. Our goal was to illuminate the biological processes and identify panels of genes associated with human variability …

An Agent-Based Model To Quantify Energetics, Movement And Habitat Selection Of Mid-Continent Mallards In The Mississippi Alluvial Valley, Florian G. Weller, Elisabeth B. Webb, Sean Fogenburg, William S. Beatty, Dylan Kesler, Robert H. Blenk, Kevin M. Ringelman, Matt L. Miller, Céline Arzel, John M. Eadie

Faculty Publications

The dynamics of wintering waterfowl populations at the landscape scale are the result of complex interactions of environmental, behavioral and energetic drivers. Agent-based models provide a method to directly link these factors in a spatially explicit framework and allow the emergence of patterns from the aggregation of individual agent actions. We adapted the Spatially-explicit Waterbird Agent-based Model Program (SWAMP), originally developed for waterfowl in central California, to simulate a basin-scale population of mallards (A. platyrhynchos) wintering in the Mississippi Alluvial Valley over a four-month period (November–February). Simulated agents move within the landscape, foraging on areas made available based on a …

Validity Of An Artificial Intelligence-Based Application To Identify Foods And Estimate Energy Intake Among Adults: A Pilot Study, Chloe P. Lozano, Emma N. Canty, Sanjoy Saha, Stephanie T. Broyles, Robbie A. Beyl, John W. Apolzan, Corby K. Martin

Clinical Research Faculty Publications

Background: The commercial application Openfit allows for automatic identification and quantification of food intake through short video capture without a physical reference marker. There are no known peer-reviewed publications on the validity of this Nutrition Artificial Intelligence (AI). Objectives: To test the validity of Openfit to identify food automatically and semiautomatically (with user correction), test the validity of Openfit at quantifying energy intake (kcal) automatically and semiautomatically, and assess satisfaction and usability of Openfit. Methods: During a laboratory-based visit, adults (7 male and 17 female), used Openfit to automatically and semiautomatically record provided meals, which were covertly weighed. Foods logged …

Characterizing The Role Of Hydraulic Retention Time On Nitrate Removal Indices In Denitrifying Bioreactors By Nonlinear Models, Yuchuan Fan, Jie Zhuang, Michael Essington, Xi Zhang, Guanghui Hua, Jehangir Bhadha, Shaopan Xia, Xuanyu Lu, Jaehoon Lee

Faculty Publications

Denitrifying bioreactors (DNBRs) are a sustainable and cost-effective practice commonly used at the edge of fields to reduce nitrate from agricultural runoff. The hydraulic retention time (HRT) is a crucial variable that affects nitrate removal rate (NRR, g N m−3 d−1), nitrate removal efficiency (NRE, %), and nitrate concentration reduction per length (Nrd, mg N L−1 m−1). In this study, two nonlinear models, the developed Michaelis-Menten (MM) model and the Mitscherlich (MT) model, were developed to characterize the relationship between nitrate removal indices (NRR, NRE, and Nrd) and HRT. This study first utilizes nonlinear models to quantitatively understand the relationship …

Responses Of Winter Wheat Yield And Water Productivity To Sowing Time And Plastic Mulching In The Loess Plateau, Lihong Wu, Hao Quan, Lina Wu, Xi Zhang, Hao Feng, Dianyuan Ding, Kadambot H.M. Siddique

Faculty Publications

On the Loess Plateau, unfavorable late-sowing conditions often arise due to late harvests from the previous season or excessive rainfall during the sowing season, which can delay seed germination, reduce tiller numbers, and decrease winter wheat yields. Few studies have explored whether plastic mulching (PM) can mitigate the adverse effects of late sowing. Consequently, we conducted a 3-year field experiment from 2017 to 2020 on the Loess Plateau combining two mulching conditions [PM and no mulching (NPM)] and three sowing times (normal, 10-day late, and 20-day late sowing). We investigated the combined influence of sowing time and mulching conditions on …

Quantitative Trait Loci, Candidate Genes, And Breeding Lines To Improve Salt Tolerance At The Flowering And Seedling Stages In Rice, Rajat Pruthi, Sandeep Chapagain, Sapphire Coronejo, Lovepreet Singh, Prasant Kumar Subudhi

Faculty Publications

Salinity is a major environmental constraint that limits growth and productivity of rice all over the world. Although rice plants are vulnerable to salt stress at both seedling and flowering stages, genetic dissection of salt tolerance at the flowering stage is challenging due to non-uniformity of stress application at the appropriate growth stage. The present study was conducted to identify quantitative trait loci (QTLs) and candidate genes associated with the flowering stage salt tolerance and compare with those associated with the seedling stage using a high-resolution genetic map. A population of advanced generation introgression lines with uniform flowering time was …

Investigating Conspecific Csrv1 Transmission In Callinectes Sapidus, Julie A. Lively, Matthew I. Spitznagel, Eric J. Schott, Hamish J. Small

Faculty Publications

A reo-like virus, CsRV1, is found in blue crabs, Callinectes sapidus, from every North American location assessed, including Chesapeake Bay and the Atlantic and Gulf coasts, USA and associated with blue crabs in softshell production. CsRV1-associated crab mortality is prevalent in captive crabs, but it is still unknown how CsRV1 is transmitted. The purpose of this study was to examine the role that conspecific predation or scavenging may play in per os transmission in single exposure and repeated exposure experiments. For viruses without cell culture propagation, repeated exposure experiments have the challenge of presenting the virus consistently during the experiment …

Low Movement, Deep-Learned Sitting Patterns, And Sedentary Behavior In The International Study Of Childhood Obesity, Lifestyle And The Environment (Iscole), Paul R. Hibbing, Jordan A. Carlson, Chelsea Steel, Mikael Anne Greenwood-Hickman, Supun Nakandala, Marta M. Jankowska, John Bellettiere, Jingjing Zou, Andrea Z. Lacroix, Arun Kumar, Peter T. Katzmarzyk, Loki Natarajan

Population and Public Health Science Faculty Publications

Background/Objectives: Sedentary behavior (SB) has both movement and postural components, but most SB research has only assessed low movement, especially in children. The purpose of this study was to compare estimates and health associations of SB when derived from a standard accelerometer cut-point, a novel sitting detection technique (CNN Hip Accelerometer Posture for Children; CHAP-Child), and both combined. Methods: Data were from the International Study of Childhood Obesity, Lifestyle, and the Environment (ISCOLE). Participants were 6103 children (mean ± SD age 10.4 ± 0.56 years) from 12 countries who wore an ActiGraph GT3X+ accelerometer on the right hip for approximately …

Gamification For Consumer Loyalty: An Exploration Of Unobserved Heterogeneity In Gamified Esports Social Live Streaming, Tyreal Yizhou Qian, Bo Yu, Robbie Matz, Lei Luo, Chenglong Xu

Faculty Publications

Esports has been boosted by the proliferation of gamified social live streaming (SLS) platforms over the past few years. While previous research has recognized the complexity of gamification, limited scholarship has examined gamification effectiveness in terms of consumer loyalty in esports. Through the lens of technology acceptance model (TAM), the current study surveyed esports consumers of two SLS platforms (n = 583) to explore the relationships between gamification dimensions, TAM determinants, and SLS platform loyalty. We examined the structural model on both aggregate and segment-specific levels and revealed the existence of unobserved heterogeneity in the model's paths and observations. The …

Bariatric Surgery Alters Mitochondrial Function In Gut Mucosa, Robert C. Ross, Elizabeth C. Heintz, Elizabeth R.M. Zunica, R. Leigh Townsend, Amanda E. Spence, Philip R. Schauer, John P. Kirwan, Christopher L. Axelrod, Vance L. Albaugh

Division of Basic Science Faculty Publications

Background: The obesity pandemic has worsened global disease burden, including type 2 diabetes, cardiovascular disease, and cancer. Metabolic/bariatric surgery (MBS) is the most effective and durable obesity treatment, but the mechanisms underlying its long-term weight loss efficacy remain unclear. MBS drives substrate oxidation that has been linked to improvements in metabolic function and improved glycemic control that are potentially mediated by mitochondria—a primary site of energy production. As such, augmentation of intestinal mitochondrial function may drive processes underlying the systemic metabolic benefits of MBS. Herein, we applied a highly sensitive technique to evaluate intestinal mitochondrial function ex vivo in a …

Access, Interest, And Barriers To Incorporation Of Birth Doula Care In The United States, Amanda W. Mitchell, Joshua R. Sparks, Robbie A. Beyl, Abby D. Altazan, S. Ariel Barlow, Leanne M. Redman

Clinical Research Faculty Publications

Doula care improves maternal care, yet barriers exist to incorporating doula care. The purpose of this study was to evaluate interest and barriers to doula care. Overall, 508 women, 26–35 years of age (54.5%), White/Caucasian (89.8%), and married (88.6%), completed this study. Most reported ≥1 previous birth (97.6%). Respondents would “feel comfortable” (73.2%) and “more confident” (54.9%) with doula care at birth, and 57.9% reported their provider would be supportive of doula care. Only 39.0% expressed benefits to doula care during pregnancy compared to 72.6% at birth and 68.1% during postpartum. Most would hire a doula if health insurance covered …

Circular-Swat For Deep Learning Based Diagnostic Classification Of Alzheimer's Disease: Application To Metabolome Data, Taeho Jo, Junpyo Kim, Paula Bice, Kevin Huynh, Tingting Wang, Matthias Arnold, Peter J. Meikle, Peter J. Meikle, Corey Giles, Rima Kaddurah-Daouk, Andrew J. Saykin, Kwangsik Nho, Alexandra Kueider-Paisley, P. Murali Doraiswamy, Colette Blach, Arthur Moseley, Will Thompson, Lisa St John-Williams, Siamak Mahmoudiandehkhordi, Jessica Tenenbaum, Kathleen Welsh-Balmer, Brenda Plassman, Shannon L. Risacher, Gabi Kastenmüller, Xianlin Han, Rebecca Baillie, Rob Knight, Pieter Dorrestein, James Brewer, Emeran Mayer, Jennifer Labus, Pierre Baldi, Arpana Gupta, Oliver Fiehn

Clinical Research Faculty Publications

Background: Deep learning has shown potential in various scientific domains but faces challenges when applied to complex, high-dimensional multi-omics data. Alzheimer's Disease (AD) is a neurodegenerative disorder that lacks targeted therapeutic options. This study introduces the Circular-Sliding Window Association Test (c-SWAT) to improve the classification accuracy in predicting AD using serum-based metabolomics data, specifically lipidomics. Methods: The c-SWAT methodology builds upon the existing Sliding Window Association Test (SWAT) and utilizes a three-step approach: feature correlation analysis, feature selection, and classification. Data from 997 participants from the Alzheimer's Disease Neuroimaging Initiative (ADNI) served as the basis for model training and validation. …

Erratum To ‘P23-075-23 Development & Validating Of A Food Literacy Behavior Questionnaire For Adults With Type 2 Diabetes Mellitus’ [Current Developments In Nutrition 7s1 (2023) 100184] (Current Developments In Nutrition (2023) 7(S1), (S2475299123249094), (10.1016/J.Cdnut.2023.100184)), Sarah Ludwig, Melissa Cater, Georgianna Tuuri

Faculty Publications

The American Society of Nutrition regrets that the author order of this abstract is incorrect. The author order is currently listed as: Georgianna Tuuri, Melissa Cater, Sarah Ludwig The author order should be: Sarah Ludwig, Melissa Cater, Georgianna Tuuri The Society would like to apologise for any inconvenience caused.

Links Between Optical And X-Ray Light In Scorpius X-1, Alexander B. Igl, R. I. Hynes, C. T. Britt, K. S. O'Brien, V. J. Mikles

Faculty Publications

We observed the low-mass X-ray binary Sco X-1 for 12 nights simultaneously using the Rossi X-Ray Timing Explorer and the Otto Struve Telescope at McDonald Observatory at 1-s time-resolution. This is among the most comprehensive simultaneous X-Ray/optical data sets of Sco X-1. Evidence of reprocessing was observed in the form of nine positive, near-zero lag peaks in the cross-correlation function, eight of which were relatively small, and took the shape of piecewise exponential functions. These peaks were initially identified by eye, after which a computational identification scheme was developed to confirm their significance. Based on their short lags (less than …

Expanding Non-Invasive Approaches For Fish-Health Monitoring: A Survey Of The Epidermal Mucous Metabolomes Of Phylogenetically Diverse Freshwater Fish Species, Drew R. Ekman, Marina G. Evich, Jonathan D. Mosley, Jon A. Doering, Kellie A. Fay, Gerald T. Ankley, Timothy W. Collette

Faculty Publications

There is a pressing need for more-holistic approaches to fisheries assessments along with growing demand to reduce the health impacts of sample collections. Metabolomic tools enable the use of sample matrices that can be collected with minimal impact on the organism (e.g., blood, urine, and mucus) and provide high-throughput, untargeted biochemical information without the requirement of a sequenced genome. These qualities make metabolomics ideal for monitoring a wide range of fish species, particularly those under protected status. In the current study, we surveyed the relative abundances of 120 endogenous metabolites in epidermal mucus across eight freshwater fish species belonging to …