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Louisiana State University

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Progenitor Cell Isolation From Mouse Epididymal Adipose Tissue And Sequencing Library Construction, Qianglin Liu, Chaoyang Li, Yuxia Li, Leshan Wang, Xujia Zhang, Buhao Deng, Peidong Gao, Mohammad Shiri, Fozi Alkaifi, Junxing Zhao, Jacqueline M. Stephens, Constantine A. Simintiras, Joseph Francis, Jiangwen Sun, Xing Fu Dec 2023

Progenitor Cell Isolation From Mouse Epididymal Adipose Tissue And Sequencing Library Construction, Qianglin Liu, Chaoyang Li, Yuxia Li, Leshan Wang, Xujia Zhang, Buhao Deng, Peidong Gao, Mohammad Shiri, Fozi Alkaifi, Junxing Zhao, Jacqueline M. Stephens, Constantine A. Simintiras, Joseph Francis, Jiangwen Sun, Xing Fu

Faculty Publications

Here, we present a protocol to isolate progenitor cells from mouse epididymal visceral adipose tissue and construct bulk RNA and assay for transposase-accessible chromatin with sequencing (ATAC-seq) libraries. We describe steps for adipose tissue collection, cell isolation, and cell staining and sorting. We then detail procedures for both ATAC-seq and RNA sequencing library construction. This protocol can also be applied to other tissues and cell types directly or with minor modifications. For complete details on the use and execution of this protocol, please refer to Liu et al. (2023).1

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Quantization Of Spherically Symmetric Loop Quantum Gravity Coupled To A Scalar Field And A Clock: The Asymptotic Limit, Rodolfo Gambini, Jorge Pullin Dec 2023

Quantization Of Spherically Symmetric Loop Quantum Gravity Coupled To A Scalar Field And A Clock: The Asymptotic Limit, Rodolfo Gambini, Jorge Pullin

Faculty Publications

We continue our work on the study of spherically symmetric loop quantum gravity coupled to two spherically symmetric scalar fields, one which acts as a clock. As a consequence of the presence of the latter, we can define a true Hamiltonian for the theory. The spherically symmetric context allows to carry out precise detailed calculations. Here we study the theory for regions of large values of the radial coordinate. This allows us to define in detail the vacuum of the theory and study its quantum states, yielding a quantum field theory on a quantum space time that makes contact with …

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Mixed Method Study On Community College Instruction In Blended Social Science Courses, Andrea L. B. Eggenberger Dec 2023

Mixed Method Study On Community College Instruction In Blended Social Science Courses, Andrea L. B. Eggenberger

LSU Doctoral Dissertations

Community colleges have teaching faculty whose primary focus is educating their students and providing service to their institution. Community college teaching research has focused on innovation, students’ expectations, and the use of active learning techniques to foster student success. Research on blended courses has focused on instructional practices, learner engagement, and the benefits of blended learning for the community college student population. This study explores the relationship between student’s perceptions of the work in a blended developmental psychology course at two community colleges in Louisiana and their professors’ instructional decisions for their blended courses. The multiple-case study uses a convergent …

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The Translational Oscillation In Oocyte And Early Embryo Development, Rajan Iyyappan, Daria Aleshkina, Hao Ming, Michal Dvoran, Kianoush Kakavand, Denisa Jansova, Edgar Del Llano, Lenka Gahurova, Alexander W. Bruce, Tomas Masek, Martin Pospisek, Filip Horvat, Michal Kubelka, Zongliang Jiang, Andrej Susor Dec 2023

The Translational Oscillation In Oocyte And Early Embryo Development, Rajan Iyyappan, Daria Aleshkina, Hao Ming, Michal Dvoran, Kianoush Kakavand, Denisa Jansova, Edgar Del Llano, Lenka Gahurova, Alexander W. Bruce, Tomas Masek, Martin Pospisek, Filip Horvat, Michal Kubelka, Zongliang Jiang, Andrej Susor

Faculty Publications

Translation is critical for development as transcription in the oocyte and early embryo is silenced. To illustrate the translational changes during meiosis and consecutive two mitoses of the oocyte and early embryo, we performed a genome-wide translatome analysis. Acquired data showed significant and uniform activation of key translational initiation and elongation axes specific to M-phases. Although global protein synthesis decreases in M-phases, translation initiation and elongation activity increases in a uniformly fluctuating manner, leading to qualitative changes in translation regulation via the mTOR1/4F/eEF2 axis. Overall, we have uncovered a highly dynamic and oscillatory pattern of translational reprogramming that contributes to …

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Environmental Dna (Edna) Removal Rates In Streams Differ By Particle Size Under Varying Substrate And Light Conditions, Elise D. Snyder, Jennifer L. Tank, Pedro F.P. Brandão-Dias, Kyle Bibby, Arial J. Shogren, Aaron W. Bivins, Brett Peters, Erik M. Curtis, Diogo Bolster, Scott P. Egan, Gary A. Lamberti Dec 2023

Environmental Dna (Edna) Removal Rates In Streams Differ By Particle Size Under Varying Substrate And Light Conditions, Elise D. Snyder, Jennifer L. Tank, Pedro F.P. Brandão-Dias, Kyle Bibby, Arial J. Shogren, Aaron W. Bivins, Brett Peters, Erik M. Curtis, Diogo Bolster, Scott P. Egan, Gary A. Lamberti

Faculty Publications

The use of environmental DNA (eDNA) as a sampling tool offers insights into the detection of invasive and/or rare aquatic species and enables biodiversity assessment without traditional sampling approaches, which are often labor-intensive. However, our understanding of the environmental factors that impact eDNA removal (i.e., how rapidly eDNA is removed from the water column by the combination of decay and physical removal) in flowing waters is limited. This limitation constrains predictions about the location and density of target organisms after positive detection. To address this question, we spiked Common Carp (Cyprinus carpio) eDNA into recirculating mesocosms (n = 24) under …

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Comparison Of Concentration And Extraction Workflows For Qpcr Quantification Of Inti1 And Vana In Untreated Wastewater, Yawen Liu, Wendy Smith, Metasebia Gebrewold, Xinhong Wang, Stuart L. Simpson, Aaron Bivins, Warish Ahmed Dec 2023

Comparison Of Concentration And Extraction Workflows For Qpcr Quantification Of Inti1 And Vana In Untreated Wastewater, Yawen Liu, Wendy Smith, Metasebia Gebrewold, Xinhong Wang, Stuart L. Simpson, Aaron Bivins, Warish Ahmed

Faculty Publications

Quantitative polymerase chain reaction (qPCR) measurement of antibiotic resistance genes (ARGs) in untreated municipal wastewater may prove useful in combating the antimicrobial resistance crisis. However, harmonizing and optimizing qPCR-based workflows is essential to facilitate comparisons across studies, and includes achieving highly-effective ARG capture through efficient concentration and extraction procedures. In the current study, combinations of sample volume, membrane types and DNA extraction kits within filtration and centrifugation-based workflows were used to quantify 16S ribosomal RNA (16S rRNA), class 1 integron-integrase gene (intI1) and an ARG encoding resistance to vancomycin (vanA) in untreated wastewater sampled from three wastewater treatment plants (WWTPs). …

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Error In Weighted Analyses And Reported Prevalence Rates, Amanda E. Staiano Dec 2023

Error In Weighted Analyses And Reported Prevalence Rates, Amanda E. Staiano

Population and Public Health Science Faculty Publications

No abstract provided.

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The Concept Of Land Bridge Marshes In The Mississippi River Delta And Implications For Coastal Restoration, John W. Day, Robert R. Twilley, Angelina Freeman, Brady Couvillion, Tracy Quirk, Navid Jafari, Giulio Mariotti, Rachael Hunter, Charles Norman, G. Paul Kemp, John R. White, Ehab Meselhe Dec 2023

The Concept Of Land Bridge Marshes In The Mississippi River Delta And Implications For Coastal Restoration, John W. Day, Robert R. Twilley, Angelina Freeman, Brady Couvillion, Tracy Quirk, Navid Jafari, Giulio Mariotti, Rachael Hunter, Charles Norman, G. Paul Kemp, John R. White, Ehab Meselhe

Faculty Publications

Louisiana has high coastal wetland loss rates due to natural processes such as subsidence and anthropogenic activities such as construction of river levees and dams, pervasive alteration of surface hydrology by local industries such as oil and gas, and navigation. With the exception of the Atchafalaya River discharge area, most of Louisiana's marsh coastline is retreating and coastal marshes are degrading. In the inactive degrading delta regions, there exists a previously uncharacterized landform referred to colloquially as coastal ‘land bridge’ marshes. Land bridge marshes are saline or brackish marshes fronting large estuarine bays or lakes with sufficient fetch and wave …

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A 30-Year Dataset Of Co2 In Flowing Freshwaters In The United States, Timothy R. Toavs, Caleb T. Hasler, Cory D. Suski, Stephen R. Midway Dec 2023

A 30-Year Dataset Of Co2 In Flowing Freshwaters In The United States, Timothy R. Toavs, Caleb T. Hasler, Cory D. Suski, Stephen R. Midway

Faculty Publications

Increasing atmospheric carbon dioxide (CO2) concentrations have been linked to effects in a wide range of ecosystems and organisms, with negative effects of elevated CO2 documented for marine organisms. Less is known about the dynamics of CO2 in freshwaters, but the potential exists for freshwater organisms to be challenged by elevated CO2. In flowing freshwaters CO2 exhibits more variability than in lakes or the ocean, yet spatiotemporally extensive direct measures of CO2 in freshwater are rare. However, CO2 can be estimated from pH, temperature, and alkalinity—commonly collected water quality metrics. We used data from the National Water Quality Monitoring Council …

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Creelcat, A Catalog Of United States Inland Creel And Angler Survey Data, Nicholas A. Sievert, Abigail J. Lynch, Holly S. Embke, Ashley Robertson, Mitchel Lang, Anna L. Kaz, Matthew D. Robertson, Stephen R. Midway, Lyndsie Wszola, Craig P. Paukert Dec 2023

Creelcat, A Catalog Of United States Inland Creel And Angler Survey Data, Nicholas A. Sievert, Abigail J. Lynch, Holly S. Embke, Ashley Robertson, Mitchel Lang, Anna L. Kaz, Matthew D. Robertson, Stephen R. Midway, Lyndsie Wszola, Craig P. Paukert

Faculty Publications

The United States Inland Creel and Angler Survey Catalog (CreelCat) contains a national compilation of angler and creel survey data collected by natural resource management agencies across the United States (including Washington, D.C. and Puerto Rico). These surveys are used to help inform the management of recreational fisheries, by collecting information about anglers including what they are catching and harvesting, the amount of effort they expend, their angling preferences, and demographic information. As of May 1, 2023, CreelCat houses over 14,729 surveys from 33 states, Puerto Rico, and Washington, D.C., comprising 235 data fields across 8 tables. These tables contain …

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Global Responses To The Covid-19 Pandemic By Recreational Anglers: Considerations For Developing More Resilient And Sustainable Fisheries, J. Robert Britton, Adrian C. Pinder, Josep Alós, Robert Arlinghaus, Andy J. Danylchuk, Wendy Edwards, Kátia M.F. Freire, Casper Gundelund, Kieran Hyder, Ivan Jarić, Robert Lennox, Wolf Christian Lewin, Abigail J. Lynch, Stephen R. Midway, Warren M. Potts, Karina L. Ryan, Christian Skov, Harry V. Strehlow, Sean R. Tracey, Jun Ichi Tsuboi, Paul A. Venturelli, Jessica L. Weir, Marc Simon Weltersbach, Steven J. Cooke Dec 2023

Global Responses To The Covid-19 Pandemic By Recreational Anglers: Considerations For Developing More Resilient And Sustainable Fisheries, J. Robert Britton, Adrian C. Pinder, Josep Alós, Robert Arlinghaus, Andy J. Danylchuk, Wendy Edwards, Kátia M.F. Freire, Casper Gundelund, Kieran Hyder, Ivan Jarić, Robert Lennox, Wolf Christian Lewin, Abigail J. Lynch, Stephen R. Midway, Warren M. Potts, Karina L. Ryan, Christian Skov, Harry V. Strehlow, Sean R. Tracey, Jun Ichi Tsuboi, Paul A. Venturelli, Jessica L. Weir, Marc Simon Weltersbach, Steven J. Cooke

Faculty Publications

The global COVID-19 pandemic resulted in many jurisdictions implementing orders restricting the movements of people to inhibit virus transmission, with recreational angling often either not permitted or access to fisheries and/or related infrastructure being prevented. Following the lifting of restrictions, initial angler surveys and licence sales suggested increased participation and effort, and altered angler demographics, but with evidence remaining limited. Here, we overcome this evidence gap by identifying temporal changes in angling interest, licence sales, and angling effort in world regions by comparing data in the ‘pre-pandemic’ (up to and including 2019); ‘acute pandemic’ (2020) and ‘COVID-acclimated’ (2021) periods. We …

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Atmospheric Flash Drought In The Caribbean, Craig A. Ramseyer, Paul W. Miller Dec 2023

Atmospheric Flash Drought In The Caribbean, Craig A. Ramseyer, Paul W. Miller

Faculty Publications

Despite the intensifying interest in flash drought both within the United States and globally, moist tropical landscapes have largely escaped the attention of the flash drought community. Because these ecozones are acclimatized to receiving regular, near-daily precipitation, they are especially vulnerable to rapid-drying events. This is particularly true within the Caribbean Sea basin where numerous small islands lack the surface and groundwater resources to cope with swiftly developing drought conditions. This study fills the tropical flash drought gap by examining the pervasiveness of flash drought across the pan-Caribbean region using a recently proposed criterion based on the evaporative demand drought …

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Nursery Origin Of Yellowfin Tuna In The Western Atlantic Ocean: Significance Of Caribbean Sea And Trans-Atlantic Migrants, Jay R. Rooker, Michelle Zapp Sluis, Larissa L. Kitchens, Michael A. Dance, Brett Falterman, Jessica M. Lee, Hui Liu, Nathaniel Miller, Hilario Murua, Alexandra M. Rooker, Eric Saillant, John Walter, R. J. David Wells Dec 2023

Nursery Origin Of Yellowfin Tuna In The Western Atlantic Ocean: Significance Of Caribbean Sea And Trans-Atlantic Migrants, Jay R. Rooker, Michelle Zapp Sluis, Larissa L. Kitchens, Michael A. Dance, Brett Falterman, Jessica M. Lee, Hui Liu, Nathaniel Miller, Hilario Murua, Alexandra M. Rooker, Eric Saillant, John Walter, R. J. David Wells

Faculty Publications

Natural geochemical markers in the otolith of yellowfin tuna (Thunnus albacares) were used to establish nursery-specific signatures for investigating the origin of fish captured in the western Atlantic Ocean (WAO). Two classes of chemical markers (trace elements, stable isotopes) were used to first establish nursery-specific signatures of age-0 yellowfin tuna from four primary production zones in the Atlantic Ocean: Gulf of Mexico, Caribbean Sea, Cape Verde, and Gulf of Guinea. Next, mixture and individual assignment methods were applied to predict the origin of sub-adult and adult yellowfin tuna from two regions in the WAO (Gulf of Mexico, Mid Atlantic Bight) …

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Soil Legacy Nutrients Contribute To The Decreasing Stoichiometric Ratio Of N And P Loading From The Mississippi River Basin, Zihao Bian, Hanqin Tian, Shufen Pan, Hao Shi, Chaoqun Lu, Christopher Anderson, Wei Jun Cai, Charles S. Hopkinson, Dubravko Justic, Latif Kalin, Steven Lohrenz, Steven Mcnulty, Naiqing Pan, Ge Sun, Zhuonan Wang, Yuanzhi Yao, Yongfa You Dec 2023

Soil Legacy Nutrients Contribute To The Decreasing Stoichiometric Ratio Of N And P Loading From The Mississippi River Basin, Zihao Bian, Hanqin Tian, Shufen Pan, Hao Shi, Chaoqun Lu, Christopher Anderson, Wei Jun Cai, Charles S. Hopkinson, Dubravko Justic, Latif Kalin, Steven Lohrenz, Steven Mcnulty, Naiqing Pan, Ge Sun, Zhuonan Wang, Yuanzhi Yao, Yongfa You

Faculty Publications

Human-induced nitrogen–phosphorus (N, P) imbalance in terrestrial ecosystems can lead to disproportionate N and P loading to aquatic ecosystems, subsequently shifting the elemental ratio in estuaries and coastal oceans and impacting both the structure and functioning of aquatic ecosystems. The N:P ratio of nutrient loading to the Gulf of Mexico from the Mississippi River Basin increased before the late 1980s driven by the enhanced usage of N fertilizer over P fertilizer, whereafter the N:P loading ratio started to decrease although the N:P ratio of fertilizer application did not exhibit a similar trend. Here, we hypothesize that different release rates of …

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Identifying Healthy Individuals With Alzheimer’S Disease Neuroimaging Phenotypes In The Uk Biobank, Tiago Azevedo, Richard A.I. Bethlehem, David J. Whiteside, Nol Swaddiwudhipong, James B. Rowe, Pietro Lió, Timothy Rittman, Gail Li, Eric C. Petrie, Elaine R. Peskind, Teresa Villena, Walter Martinez, Carl Sadowsky, Athena Lee, Lisa Ravdin, Michael Lin, Gloria Chiang, Norman Relkin, Robert B. Santulli, Mary L. Hynes, Marc Seltzer, Laura A. Flashman, Karen Anderson, Karen Blank, Godfrey D. Pearlson, Smita Kittur, Stephen Correia, Paul Malloy, Stephen Salloway, Steven Potkin, Paul Newhouse, Angelica Garcia, Jiong Shi, Joseph Masdeu Dec 2023

Identifying Healthy Individuals With Alzheimer’S Disease Neuroimaging Phenotypes In The Uk Biobank, Tiago Azevedo, Richard A.I. Bethlehem, David J. Whiteside, Nol Swaddiwudhipong, James B. Rowe, Pietro Lió, Timothy Rittman, Gail Li, Eric C. Petrie, Elaine R. Peskind, Teresa Villena, Walter Martinez, Carl Sadowsky, Athena Lee, Lisa Ravdin, Michael Lin, Gloria Chiang, Norman Relkin, Robert B. Santulli, Mary L. Hynes, Marc Seltzer, Laura A. Flashman, Karen Anderson, Karen Blank, Godfrey D. Pearlson, Smita Kittur, Stephen Correia, Paul Malloy, Stephen Salloway, Steven Potkin, Paul Newhouse, Angelica Garcia, Jiong Shi, Joseph Masdeu

Clinical Research Faculty Publications

Spotting people with dementia early is challenging, but important to identify people for trials of treatment and prevention. We used brain scans of people with Alzheimer’s disease, the commonest type of dementia, and applied an artificial intelligence method to spot people with Alzheimer’s disease. We used this to find people in the Healthy UK Biobank study who might have early Alzheimer’s disease. The people we found had subtle changes in their memory and thinking to suggest they may have early disease, and we also found they had high blood pressure and smoked for longer. We have demonstrated an approach that …

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Refining The Diagnosis Of Gestational Diabetes Mellitus: A Systematic Review And Meta-Analysis, Ellen C. Francis, Camille E. Powe, William L. Lowe, Sara L. White, Sara L. White, Denise M. Scholtens, Jiaxi Yang, Yeyi Zhu, Cuilin Zhang, Marie France Hivert, Marie France Hivert, Soo Heon Kwak, Arianne Sweeting, Paul W. Franks, Stephen S. Rich, Robert Wagner, Tina Vilsbøll, Kimberly K. Vesco, Miriam S. Udler, Tiinamaija Tuomi, Emily K. Sims, Jennifer L. Sherr, Robert K. Semple, Rebecca M. Reynolds, Maria J. Redondo, Leanne M. Redman, Richard E. Pratley, Rodica Pop-Busui, Toni I. Pollin, Wei Perng, Ewan R. Pearson, Susan E. Ozanne, Katharine R. Owen, Richard Oram Dec 2023

Refining The Diagnosis Of Gestational Diabetes Mellitus: A Systematic Review And Meta-Analysis, Ellen C. Francis, Camille E. Powe, William L. Lowe, Sara L. White, Sara L. White, Denise M. Scholtens, Jiaxi Yang, Yeyi Zhu, Cuilin Zhang, Marie France Hivert, Marie France Hivert, Soo Heon Kwak, Arianne Sweeting, Paul W. Franks, Stephen S. Rich, Robert Wagner, Tina Vilsbøll, Kimberly K. Vesco, Miriam S. Udler, Tiinamaija Tuomi, Emily K. Sims, Jennifer L. Sherr, Robert K. Semple, Rebecca M. Reynolds, Maria J. Redondo, Leanne M. Redman, Richard E. Pratley, Rodica Pop-Busui, Toni I. Pollin, Wei Perng, Ewan R. Pearson, Susan E. Ozanne, Katharine R. Owen, Richard Oram

Clinical Research Faculty Publications

Background: Perinatal outcomes vary for women with gestational diabetes mellitus (GDM). The precise factors beyond glycemic status that may refine GDM diagnosis remain unclear. We conducted a systematic review and meta-analysis of potential precision markers for GDM. Methods: Systematic literature searches were performed in PubMed and EMBASE from inception to March 2022 for studies comparing perinatal outcomes among women with GDM. We searched for precision markers in the following categories: maternal anthropometrics, clinical/sociocultural factors, non-glycemic biochemical markers, genetics/genomics or other -omics, and fetal biometry. We conducted post-hoc meta-analyses of a subset of studies with data on the association of maternal …

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Utility And Precision Evidence Of Technology In The Treatment Of Type 1 Diabetes: A Systematic Review, Laura M. Jacobsen, Jennifer L. Sherr, Elizabeth Considine, Angela Chen, Sarah M. Peeling, Margo Hulsmans, Sara Charleer, Marzhan Urazbayeva, Mustafa Tosur, Selma Alamarie, Maria J. Redondo, Korey K. Hood, Peter A. Gottlieb, Pieter Gillard, Jessie J. Wong, Irl B. Hirsch, Richard E. Pratley, Lori M. Laffel, Chantal Mathieu, Paul W. Franks, Stephen S. Rich, Robert Wagner, Tina Vilsbøll, Kimberly K. Vesco, Miriam S. Udler, Tiinamaija Tuomi, Arianne Sweeting, Emily K. Sims, Robert K. Semple, Rebecca M. Reynolds, Leanne M. Redman, Rodica Pop-Busui, Toni I. Pollin, Wei Perng Dec 2023

Utility And Precision Evidence Of Technology In The Treatment Of Type 1 Diabetes: A Systematic Review, Laura M. Jacobsen, Jennifer L. Sherr, Elizabeth Considine, Angela Chen, Sarah M. Peeling, Margo Hulsmans, Sara Charleer, Marzhan Urazbayeva, Mustafa Tosur, Selma Alamarie, Maria J. Redondo, Korey K. Hood, Peter A. Gottlieb, Pieter Gillard, Jessie J. Wong, Irl B. Hirsch, Richard E. Pratley, Lori M. Laffel, Chantal Mathieu, Paul W. Franks, Stephen S. Rich, Robert Wagner, Tina Vilsbøll, Kimberly K. Vesco, Miriam S. Udler, Tiinamaija Tuomi, Arianne Sweeting, Emily K. Sims, Robert K. Semple, Rebecca M. Reynolds, Leanne M. Redman, Rodica Pop-Busui, Toni I. Pollin, Wei Perng

Clinical Research Faculty Publications

Background: The greatest change in the treatment of people living with type 1 diabetes in the last decade has been the explosion of technology assisting in all aspects of diabetes therapy, from glucose monitoring to insulin delivery and decision making. As such, the aim of our systematic review was to assess the utility of these technologies as well as identify any precision medicine-directed findings to personalize care. Methods: Screening of 835 peer-reviewed articles was followed by systematic review of 70 of them (focusing on randomized trials and extension studies with ≥50 participants from the past 10 years). Results: We find …

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A Scalable, Virtual Weight Management Program Tailored For Adults With Type 2 Diabetes: Effects On Glycemic Control, John W. Apolzan, Jessica Gokee Larose, Stephen D. Anton, Robbie A. Beyl, Frank L. Greenway, Edmond P. Wickham, Autumn Lanoye, Melissa N. Harris, Corby K. Martin, Tiffany Bullard, Gary D. Foster, Michelle I. Cardel Dec 2023

A Scalable, Virtual Weight Management Program Tailored For Adults With Type 2 Diabetes: Effects On Glycemic Control, John W. Apolzan, Jessica Gokee Larose, Stephen D. Anton, Robbie A. Beyl, Frank L. Greenway, Edmond P. Wickham, Autumn Lanoye, Melissa N. Harris, Corby K. Martin, Tiffany Bullard, Gary D. Foster, Michelle I. Cardel

Clinical Research Faculty Publications

Background: The objective was to test the efficacy of a scalable, virtually delivered, diabetes-tailored weight management program on glycemic control in adults with type 2 diabetes (T2D). Methods: This was a single arm, three-site clinical trial. Participants had baseline HbA1c between 7–11% and BMI between 27–50 kg/m2. Primary outcome was change in HbA1c at 24 weeks. Secondary outcomes were changes in body weight, waist circumference, the Diabetes Distress Scale (DDS), quality of life (IWQOL-L), and hunger (VAS). Generalized linear effects models were used for statistical analysis. Results: Participants (n = 136) were 56.8 ± 0.8 y (Mean ± SEM), 36.9 …

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Uncovering The Environmental Conditions Required For Phyllachora Maydis Infection And Tar Spot Development On Corn In The United States For Use As Predictive Models For Future Epidemics, Richard W. Webster, Camila Nicolli, Tom W. Allen, Mandy D. Bish, Kaitlyn Bissonnette, Jill C. Check, Martin I. Chilvers, Maíra R. Duffeck, Nathan Kleczewski, Jane Marian Luis, Brian D. Mueller, Pierce A. Paul, Paul P. Price, Alison E. Robertson, Tiffanna J. Ross, Clarice Schmidt, Roger Schmidt, Teryl Schmidt, Sujoung Shim, Darcy E.P. Telenko, Kiersten Wise, Damon L. Smith Dec 2023

Uncovering The Environmental Conditions Required For Phyllachora Maydis Infection And Tar Spot Development On Corn In The United States For Use As Predictive Models For Future Epidemics, Richard W. Webster, Camila Nicolli, Tom W. Allen, Mandy D. Bish, Kaitlyn Bissonnette, Jill C. Check, Martin I. Chilvers, Maíra R. Duffeck, Nathan Kleczewski, Jane Marian Luis, Brian D. Mueller, Pierce A. Paul, Paul P. Price, Alison E. Robertson, Tiffanna J. Ross, Clarice Schmidt, Roger Schmidt, Teryl Schmidt, Sujoung Shim, Darcy E.P. Telenko, Kiersten Wise, Damon L. Smith

Faculty Publications

Phyllachora maydis is a fungal pathogen causing tar spot of corn (Zea mays L.), a new and emerging, yield-limiting disease in the United States. Since being first reported in Illinois and Indiana in 2015, P. maydis can now be found across much of the corn growing regions of the United States. Knowledge of the epidemiology of P. maydis is limited but could be useful in developing tar spot prediction tools. The research presented here aims to elucidate the environmental conditions necessary for the development of tar spot in the field and the creation of predictive models to anticipate future tar …

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The Influence Of Salinity And Vegetation Texture On The Ecological Roles Of Insects In Tidal Marshes In Louisiana, Benjamin G. Aker, Claudia Husseneder, Lane D. Foil Dec 2023

The Influence Of Salinity And Vegetation Texture On The Ecological Roles Of Insects In Tidal Marshes In Louisiana, Benjamin G. Aker, Claudia Husseneder, Lane D. Foil

Faculty Publications

Tidal marsh insect communities are influenced by both salinity and aspects of vegetation texture (vegetation diversity, plant density, and architectural structure). These factors affect the abundances and intra- and interguild interactions of these insects and should result in broad-scale variation in the distribution and ecological functioning of tidal marsh insect communities along these gradients. However, this assumption has not been tested within Gulf Coast marshes, and the insect communities and their ecological roles are not well known. This study identifies how family-level insect biodiversity varies by salinity and which factors are most important in affecting the distribution of insect functional …

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A Survey On Mosquito Control Knowledge And Insecticide Use In New Orleans, La, 2020–2021, Erin R. Cloherty, Janet C. Mcallister, James A. Ottea, Kristen Healy, Claudia Riegel Dec 2023

A Survey On Mosquito Control Knowledge And Insecticide Use In New Orleans, La, 2020–2021, Erin R. Cloherty, Janet C. Mcallister, James A. Ottea, Kristen Healy, Claudia Riegel

Faculty Publications

Mosquitoes are a known public nuisance and can vector various diseases. Historically, New Orleans, LA, has long been acquainted with the burden of mosquito-borne diseases, such as malaria and yellow fever in the 20th century and West Nile virus in the 21st century. Government mosquito control awareness campaigns have been around for decades as has the use of organophosphate and pyrethroid insecticides by mosquito abatement districts. However, few data are available on public perception of mosquito control and public usage of insecticides to kill mosquitoes in New Orleans. We conducted a survey from August 2020 to July 2021 to evaluate …

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Droid: Dose-Ranging Approach To Optimizing Dose In Oncology Drug Development, Beibei Guo, Ying Yuan Dec 2023

Droid: Dose-Ranging Approach To Optimizing Dose In Oncology Drug Development, Beibei Guo, Ying Yuan

Faculty Publications

In the era of targeted therapy, there has been increasing concern about the development of oncology drugs based on the “more is better” paradigm, developed decades ago for chemotherapy. Recently, the US Food and Drug Administration (FDA) initiated Project Optimus to reform the dose optimization and dose selection paradigm in oncology drug development. To accommodate this paradigm shifting, we propose a dose-ranging approach to optimizing dose (DROID) for oncology trials with targeted drugs. DROID leverages the well-established dose-ranging study framework, which has been routinely used to develop non-oncology drugs for decades, and bridges it with established oncology dose-finding designs to …

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Discovery Of A New Highly Pathogenic Toxin Involved In Insect Sepsis, Yuan Zhang, Hao Li, Fang Wang, Chang Liu, Gadi V.P. Reddy, Hu Li, Zhihong Li, Yucheng Sun, Zihua Zhao Dec 2023

Discovery Of A New Highly Pathogenic Toxin Involved In Insect Sepsis, Yuan Zhang, Hao Li, Fang Wang, Chang Liu, Gadi V.P. Reddy, Hu Li, Zhihong Li, Yucheng Sun, Zihua Zhao

Faculty Publications

Insect sepsis is a severe consequence that arises from the invasion of the hemocoel by symbionts of entomopathogenic nematodes and bacteria. In the present study, we unveiled the heightened virulence of the entomopathogenic nematode Steinernema feltiae and the entomopathogenic bacteria Xenorhabdus bovienii, which operate symbiotically, against the wax moth Galleria mellonella. Maximum mortality was observed at 25°C while the optimal infestation efficiency was 20 nematodes per host. After infestation, G. mellonella displayed rapid darkening and softening, accompanied by an escalated esterase activity at 9 h. The X. bovienii, released by S. feltiae, underwent substantial proliferation and discharged toxins that attacked …

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A Chromosome Scale Assembly Of The Tarnished Plant Bug, Lygus Lineolaris (Palisot De Beauvois), Genome, O. P. Perera, Surya Saha, James Glover, Katherine A. Parys, K. Clint Allen, Snejana Grozeva, Ryan Kurtz, Gadi V.P. Reddy, J. Spencer Johnston, Mark Daly, Thomas Swale Dec 2023

A Chromosome Scale Assembly Of The Tarnished Plant Bug, Lygus Lineolaris (Palisot De Beauvois), Genome, O. P. Perera, Surya Saha, James Glover, Katherine A. Parys, K. Clint Allen, Snejana Grozeva, Ryan Kurtz, Gadi V.P. Reddy, J. Spencer Johnston, Mark Daly, Thomas Swale

Faculty Publications

Objective: The tarnished plant bug (TPB), Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae), is a pest damaging many cultivated crops in North America. Although partial transcriptome data are available for this pest, a genome assembly was not available for this species. This assembly of a high-quality chromosome-length genome of TPB is aimed to develop the genetic resources that can provide the foundation required for advancing research on this species. Results: The initial genome of TPB assembled with paired-end nucleotide sequences generated with Illumina technology was scaffolded with Illumina HiseqX reads generated from a proximity ligated (HiC) library to obtain a …

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Taxonomic Profiling Of Nasutitermes Takasagoensis Microbiota To Investigate The Role Of Termites As Vectors Of Bacteria Linked To Ironwood Tree Decline In Guam, Garima Setia, Junyan Chen, Robert Schlub, Claudia Husseneder Dec 2023

Taxonomic Profiling Of Nasutitermes Takasagoensis Microbiota To Investigate The Role Of Termites As Vectors Of Bacteria Linked To Ironwood Tree Decline In Guam, Garima Setia, Junyan Chen, Robert Schlub, Claudia Husseneder

Faculty Publications

The ironwood tree (Casuarina equisetifolia, family Casuarinaceae), an indigenous agroforestry species in Guam, has been threatened by ironwood tree decline (IWTD) since 2002. Formation of bacterial ooze by the wilt pathogen from the Ralstonia solanacearum species complex and wetwood bacteria (primarily Klebsiella species) has been linked to IWTD. In addition, termite infestation of trees was statistically associated with IWTD. Termites are known carriers of a diverse microbiome. Therefore, we hypothesized that termites could be vectors of bacteria linked to IWTD. To investigate the potential role of termites as pathogen vectors, we employed next-generation 16S rRNA gene sequencing to describe the …

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Right Frontal Hd-Tdcs Reveals Causal Involvement Of Time Perception Networks In Temporal Processing Of Concepts, Karim Johari, Fatemeh Tabari, Rutvik H. Desai Dec 2023

Right Frontal Hd-Tdcs Reveals Causal Involvement Of Time Perception Networks In Temporal Processing Of Concepts, Karim Johari, Fatemeh Tabari, Rutvik H. Desai

Faculty Publications

Evidence suggests that perceptual and action related features of concepts are grounded in the corresponding sensory-motor networks in the human brain. However, less is known about temporal features of event concepts (e.g., a lecture) and whether they are grounded in time perception networks. We examined this question by stimulating the right dorsolateral prefrontal cortex (rDLPFC)—a part of time perception network—using HD-tDCS and subsequently recording EEG while participants performed semantic and time perception tasks. Semantic tasks were composed of event noun duration judgment (EDur), object noun size judgement (OSize), event (EVal) and object noun valence judgement. In the time perception task, …

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A Repeated-Measures Study Of Relational Turbulence And Transition Processing Communication During The Summer Of Covid-19 (Summer 2020), Kellie St Cyr Brisini, Denise Haunani Solomon Dec 2023

A Repeated-Measures Study Of Relational Turbulence And Transition Processing Communication During The Summer Of Covid-19 (Summer 2020), Kellie St Cyr Brisini, Denise Haunani Solomon

Faculty Publications

Drawing upon relational turbulence theory and the experiencing life transitions model, this study examined communication and relationship qualities as married couples experienced work-family changes during the COVID-19 pandemic. Sixty-four American married, different-sex partners affected by job-related changes due to COVID-19 reported their relationship qualities and their own and their spouse’s engagement in transition processing communication weekly for 10 weeks. Hypotheses addressed associations between relationship qualities and communication experiences both averaged across the 10 weeks and indexed by within-week deviations from those averages, controlling for the previous week’s reports. Husbands’ and wives’ own attempts—and also their perceptions of their spouse’s attempts—to …

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Linking Stress To The Emergence Of Relational Turbulence In Marriage, Kellie St Cyr Brisini, Denise Haunani Solomon, Miriam Brinberg Dec 2023

Linking Stress To The Emergence Of Relational Turbulence In Marriage, Kellie St Cyr Brisini, Denise Haunani Solomon, Miriam Brinberg

Faculty Publications

The assumption that stress negatively impacts marital relationships is widely accepted; however, the majority of research has focused on marital satisfaction as the outcome of interest. Relational turbulence is a quality of romantic associations on par with—but distinct from—satisfaction, in which partners conceptualize their relationship as chaotic or tumultuous. This paper draws on relational turbulence theory (RTT) and stress spillover research to propose that day-to-day stress corresponds with perceptions of relational uncertainty and interdependence, which contributes to increases in relational turbulence. We evaluated these assumptions using data from 64 heterosexual married partners who experienced work-related disruptions due to the COVID-19 …

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Building Resilience To Extreme Weather And Climate Events In The Rural Water And Wastewater Sectors, Nathan P. Kettle, Sarah F. Trainor, Renee Edwards, Donald Antrobus, Curt Baranowski, Tina Buxbaum, Kevin Berry, Michael Brubaker, Kristine L. De Long, Steve Fries, Davin Holen, Barry Keim, Danielle Meeker, Harry Penn, Cheryl Rosa, Rick Thoman, John Walsh, Jian Zhang Dec 2023

Building Resilience To Extreme Weather And Climate Events In The Rural Water And Wastewater Sectors, Nathan P. Kettle, Sarah F. Trainor, Renee Edwards, Donald Antrobus, Curt Baranowski, Tina Buxbaum, Kevin Berry, Michael Brubaker, Kristine L. De Long, Steve Fries, Davin Holen, Barry Keim, Danielle Meeker, Harry Penn, Cheryl Rosa, Rick Thoman, John Walsh, Jian Zhang

Faculty Publications

Extreme weather and climate events pose significant risks to rural water and wastewater systems. We examine the vulnerability of the water sector to weather and climate extremes in rural, predominantly Indigenous and underserved coastal areas and analyze how networks support resilience. Drawing on the analysis of 39 web-based questionnaire responses and 19 interviews with rural water and wastewater managers and service providers in southern Louisiana and western Alaska, this article reports a range of interrelated historical, environmental, and social factors that influence vulnerability to extreme weather events. Formal and informal social networks serve multiple roles in building resilience. These roles …

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Mechanical And Thermal Properties Of Mud Dauber Nests Under Atmospheric Drying, Joon S. Park, Hai Lin, Hussein Alqrinawi Dec 2023

Mechanical And Thermal Properties Of Mud Dauber Nests Under Atmospheric Drying, Joon S. Park, Hai Lin, Hussein Alqrinawi

Faculty Publications

Mud dauber wasps construct soil nests to protect their offspring from predators, extreme temperatures, and rainwater. The mechanical and thermal properties of these nests are important for the reproductive success of mud daubers. The previous study showed that the high densities and strengths of mud dauber nests were due to the repetitive tapping and atmospheric drying used by mud daubers during nest construction. This study investigated the effect of atmospheric drying on the mechanical and thermal properties of mud dauber nests. The soil shrinkage curve, elastic modulus, suction stress characteristic curve, soil water retention curve, shear strength, and thermal conductivity …

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