Digital Commons Network^™

Channel Expansion In The Ligand-Binding Domain Of The Glucocorticoid Receptor Contributes To The Activity Of Highly Potent Glucocorticoid Analogues, Wesley B. Seaton, Susan J. Burke, Alexander R. Fisch, William A. Schilletter, Mary Grace A. Beck, Gabrielle A. Cassagne, Innocence Harvey, Molly S. Fontenot, J. Jason Collier, Shawn R. Campagna

Division of Basic Science Faculty Publications

Glucocorticoids (GCs) act through the glucocorticoid receptor (GR) and are commonly used as anti-inflammatory and immunosuppressant medications. Chronic GC use has been linked with unwanted complications such as steroid-induced diabetes mellitus (SIDM), although the mechanisms for these effects are not completely understood. Modification of six GC parent molecules with 2-mercaptobenzothiazole resulted in consistently less promoter activity in transcriptional activation assays using a 3xGRE reporter construct while constantly reducing inflammatory pathway activity. The most selective candidate, DX1, demonstrated a significant reduction (87%) in transactivation compared to commercially available dexamethasone. DX1 also maintained 90% of the anti-inflammatory potential of dexamethasone while simultaneously …

Functional And Multi-Omics Effects Of An Optimized Crispr-Mediated Furin Depletion In U937 Monocytes, Ruiming Chua, Lijin Wang, Roshni Singaraja, Sujoy Ghosh

Division of Basic Science Faculty Publications

The pro-protein convertase FURIN (PCSK3) is implicated in a wide range of normal and pathological biological processes such as infectious diseases, cancer and cardiovascular diseases. Previously, we performed a systemic inhibition of FURIN in a mouse model of atherosclerosis and demonstrated significant plaque reduction and alterations in macrophage function. To understand the cellular mechanisms affected by FURIN inhibition in myeloid cells, we optimized a CRISPR-mediated gene deletion protocol for successfully deriving hemizygous (HZ) and nullizygous (NZ) FURIN knockout clones in U937 monocytic cells using lipotransfection-based procedures and a dual guide RNA delivery strategy. We observed differences in monocyte and macrophage …

Pregnane X Receptor Knockout Mitigates Weight Gain And Hepatic Metabolic Dysregulation In Female C57bl/6 J Mice On A Long-Term High-Fat Diet, Lidya H. Gebreyesus, Sora Choi, Prince Neequaye, Mattia Mahmoud, Mia Mahmoud, Malvin Ofosu-Boateng, Elizabeth Twum, Daniel O. Nnamani, Lijin Wang, Nour Yadak, Sujoy Ghosh, Frank J. Gonzalez, Maxwell A. Gyamfi

Division of Basic Science Faculty Publications

Obesity is a significant risk factor for several chronic diseases. However, pre-menopausal females are protected against high-fat diet (HFD)-induced obesity and its adverse effects. The pregnane X receptor (PXR, NR1I2), a xenobiotic-sensing nuclear receptor, promotes short-term obesity-associated liver disease only in male mice but not in females. Therefore, the current study investigated the metabolic and pathophysiological effects of a long-term 52-week HFD in female wild-type (WT) and PXR-KO mice and characterized the PXR-dependent molecular pathways involved. After 52 weeks of HFD ingestion, the body and liver weights and several markers of hepatotoxicity were significantly higher in WT mice than in …

Tai Chi Counteracts Age-Related Somatosensation And Postural Control Declines Among Older Adults, Teng Zhang, Li Li, Jan M. Hondzinski, Min Mao, Wei Sun, Qipeng Song

Faculty Publications

Objective: To investigate the effect of a 16-week Tai Chi practice on strength, tactile sensation, kinesthesia, and static postural control among older adults of different age groups. Methods: This is a quasi-experimental study. Thirteen participants aged 60–69 years (60–69yr), 11 aged 70–79 years (70–79yr), and 13 aged 80–89 years (80–89yr) completed 16 weeks of 24-form Tai Chi practice. Their ankle and hip peak torque, tactile sensation, ankle and knee kinesthesia, and the root mean square of the center of pressure (Cop-RMS) were measured before (week 0) and after (week 17) practice. Results: 80–89yr showed less ankle plantar/dorsiflexion and hip abduction …

Human Milk Components And The Infant Gut Microbiome At 6 Months: Understanding The Interconnected Relationship, Maryam Kebbe, Kartik Shankar, Leanne M. Redman, Aline Andres

Clinical Research Faculty Publications

Background: Human milk oligosaccharides have been shown to relate to the infant gut microbiome. However, the impact of other human milk components on infant gut bacterial colonization remains unexplored. Objectives: Our cross-sectional analysis aimed to investigate associations between human milk components (energy, macronutrients, free amino acids, inflammatory markers, and hormones) and infant gut microbiome diversity and composition (phylum, family, and genus) at 6 mo of age. Methods: Human milk and infant stool samples were collected at 6 mo postpartum. The infant gut microbiome was profiled using 16S rRNA sequencing. Linear regression models were performed to examine associations, adjusting for pregravid …

Prevalence And Predictors Of Female Sexual Dysfunction Among Sexually Active Women In The Diabetes Prevention Program Outcomes Study, Priyanka Gupta, Lindsay Doherty, Marinella Temprosa, Rodica Pop-Busui, Kishore M. Gadde, Prachi Singh, Arthur H. Owora, Hunter Wessells, Aruna V. Sarma

Clinical Research Faculty Publications

Objective: To determine the burden and identify correlates of female sexual dysfunction (FSD) among women with prediabetes (PreD) and type 2 diabetes (T2D) enrolled in the Diabetes Prevention Program (DPP) Outcomes Study (DPPOS). Methods: The DPPOS visit included the Female Sexual Function Index (FSFI) to determine sexual function. Of 1464 participants, 1320 (90%) completed the (FSFI) and 426 were sexually active. A backward selection multivariable logistic regression model estimated the odds of FSD for sociodemographic, clinical, and diabetes-related covariates. Results: One hundred and eighty-five (43%) had a score of ≤26.55 and met the criteria for FSD. After adjustment for DPP …

Embryonic Exposure To Benzotriazole Ultraviolet Stabilizer 327 Alters Behavior Of Rainbow Trout Alevin, Andreas N.M. Eriksson, Justin Dubiel, Lauren Zink, Zhe Lu, Jon A. Doering, Steve Wiseman

Faculty Publications

Benzotriazole ultraviolet (UV) stabilizers (BUVSs) are used in great quantities during industrial production of a variety of consumer and industrial goods. As a result of leaching and spill, BUVSs are detectable ubiquitously in the environment. As of May 2023, citing concerns related to bioaccumulation, biomagnification, and environmental persistence, (B)UV(S)-328 was recommended to be listed under Annex A of the Stockholm Convention on Persistent Organic Pollutants. However, a phaseout of UV-328 could result in a regrettable substitution because the replacement chemical(s) could cause similar or unpredicted toxicity in vivo, relative to UV-328. Therefore, the influence of UV-327, a potential replacement of …

Evaluation Of Organic And Synthetic Herbicide Applications On Weed Suppression In A Conventional Cropping System In Louisiana, Caitlin Denux, Aixin Hou, Lisa Fultz

Faculty Publications

Synthetic herbicides, with their varying modes of action, are well known for their efficiency in the suppression and control of weed species in U.S. agriculture. However, the consequences of using synthetic herbicides without attention to the surrounding environment produce chemical run-off, changes in soil health and soil health conditions, and create herbicide-resistant weeds. These outcomes have encouraged growers to seek alternative methods for their weed management programs or farming operations. Organic production systems and organic pesticides have helped address these challenges related to sustainability and environmental health. However, the use of organic herbicides in a conventional cropping system is not …

A Joint Fermi-Gbm And Swift-Bat Analysis Of Gravitational-Wave Candidates From The Third Gravitational-Wave Observing Run, C. Fletcher, J. Wood, R. Hamburg, P. Veres, C. M. Hui, E. Bissaldi, M. S. Briggs, E. Burns, W. H. Cleveland, M. M. Giles, A. Goldstein, B. A. Hristov, D. Kocevski, S. Lesage, B. Mailyan, C. Malacaria, S. Poolakkil, A. Von Kienlin, C. A. Wilson-Hodge, M. Crnogorčević, J. Delaunay, A. Tohuvavohu, R. Caputo, S. B. Cenko, S. Laha, T. Parsotan, R. Abbott, H. Abe, F. Acernese, K. Ackley, N. Adhikari, R. X. Adhikari, V. K. Adkins

Faculty Publications

We present Fermi Gamma-ray Burst Monitor (Fermi-GBM) and Swift Burst Alert Telescope (Swift-BAT) searches for gamma-ray/X-ray counterparts to gravitational-wave (GW) candidate events identified during the third observing run of the Advanced LIGO and Advanced Virgo detectors. Using Fermi-GBM onboard triggers and subthreshold gamma-ray burst (GRB) candidates found in the Fermi-GBM ground analyses, the Targeted Search and the Untargeted Search, we investigate whether there are any coincident GRBs associated with the GWs. We also search the Swift-BAT rate data around the GW times to determine whether a GRB counterpart is present. No counterparts are found. Using both the Fermi-GBM Targeted Search …

Magnetic Properties Of B Doped Mn-Ga-C Based Alloys, I. Dubenko, A. Oli, J. Duncan, A. Granovsky, R. Razhabov, M. Hill, Yu Koshkid'ko, S. Stadler, S. Talapatra, N. Ali

Faculty Publications

We present results on the structural and magnetic properties of the B-doped Mn60Ga20C20-xBx system with x = 0, 1, 2, 3, 4 and 5. A ball milling technique followed by pressing the components, and annealing under an ultra-high purity argon atmosphere for 2 h was employed to fabricate the samples. According to the XRD diffraction data, all samples can be described as single-phase compounds (i.e., the Mn3Ga cubic phase in the Pm–3 m space group) where impurity phases did not exceed 5 %. The compounds with x < 5 show similar physical behaviors as the parent, Mn3Ga2C2, which can be generally characterized by a low temperature transition from low to high magnetization states. High boron concentrations were found to completely suppress the antiferromagnetic state. The (x-TC) phase diagram at 10 K has been constructed and discussed. The magnetocaloric measurements result in magnetic entropy changes of 3.0 Jkg^-1k⁻¹ at 5 T and ΔTad by direct measurement of about …

Biochar-Based Materials As Remediation Strategy In Petroleum Hydrocarbon-Contaminated Soil And Water: Performances, Mechanisms, And Environmental Impact, Zhuo Wei, Yi Wei, Yang Liu, Shuai Niu, Yaxi Xu, Jong Hwan Park, Jim J. Wang

Faculty Publications

Petroleum contamination is considered as a major risk to the health of humans and environment. Biochars as low-cost and eco-friendly carbon materials, have been widely used for the removal of petroleum hydrocarbon in the environment. The purpose of this paper is to review the performance, mechanisms, and potential environmental toxicity of biochar, modified biochar and its integration use with other materials in petroleum contaminated soil and water. Specifically, the use of biochar in oil-contaminated water and soil as well as the factors that could influence the removal ability of biochar were systematically evaluated. In addition, the modification and integrated use …

Teenage Blues: Predictors Of Depression Among Adolescents In Nigeria, Adefunke Dadematthews, Chukwuemeka Nzeakah, Lucky Onofa, Oluwagbemiga Dadematthews, Temitope Ogundare

Faculty Publications

Background Depressive disorders, with a prevalence of 15-21%, are among the most common disorders in children and adolescents, and increases the risk of suicide, the second leading cause of death in children aged 10 to 19. Aim To determine the prevalence and correlates of depressive disorders among senior students attending secondary schools in Abeokuta. Method The study was conducted in five schools randomly selected from a representative sample and was carried out in 2 phases. In the first phase, students were selected via systematic random sampling and given consent forms and GHQ-12 to administer to the parents. In the second …

Impacts Of Extreme Weather Events On Mortgage Risks And Their Evolution Under Climate Change: A Case Study On Florida, Raffaella Calabrese, Timothy Dombrowski, Antoine Mandel, R. Kelley Pace, Luca Zanin

Faculty Publications

We develop an additive Cox proportional hazard model with time-varying covariates, including spatio-temporal characteristics of weather events, to study the impact of weather extremes (heavy rains and tropical cyclones) on the probability of mortgage default and prepayment. We compare the survival model with a flexible logistic model and an extreme gradient boosting algorithm. We estimate the models on a portfolio of mortgages in Florida, consisting of 69,046 loans and 3,707,831 loan-month observations with localization data at the five-digit ZIP code level. We find a statistically significant and non-linear impact of tropical cyclone intensity on default as well as a significant …

Building Better Writing Skills In Students With Emotional And Behavioral Disorders: About The Special Series, Paul Mooney, Joseph B. Ryan

Faculty Publications

No abstract provided.

The Association Between Diet Quality And Glycemic Outcomes Among People With Type 1 Diabetes, Melanie B. Gillingham, Martin Chase Marak, Michael C. Riddell, Peter Calhoun, Robin L. Gal, Susana R. Patton, Peter G. Jacobs, Jessica R. Castle, Mark A. Clements, Francis J. Doyle, Michael R. Rickels, Corby K. Martin

Clinical Research Faculty Publications

Background: The amount and type of food consumed impacts the glycemic response and insulin needs of people with type 1 diabetes mellitus (T1DM). Daily variability in consumption, reflected in diet quality, may acutely impact glycemic levels and insulin needs. Objective: Type 1 Diabetes Exercise Initiative (T1DEXI) data were examined to evaluate the impact of daily diet quality on near-term glycemic control and interaction with exercise. Methods: Using the Remote Food Photography Method, ≤8 d of dietary intake data were analyzed per participant. Diet quality was quantified with the Healthy Eating Index-2015 (HEI), where a score of 100 indicates the highest-quality …

Effects Of Wind On The Stability Of Explosive Eruption Plumes, Tyler Paladino, Shannon Kobs Nawotniak, Bryan Nicholson, Suniti Karunatillake

Faculty Publications

Explosive volcanic eruptions can produce eruption plumes and pyroclastic density currents that can severely damage life and property. While many factors influence eruption plume stability, including mass eruption rate, grain size distribution, and environmental factors, the role of wind is complicated, with evidence for wind as both a stabilizing and destabilizing factor. Here we use the Active Tracer High-resolution Atmospheric Model (ATHAM) to simulate 980 plumes with differing eruptive parameters, atmospheric profiles, and wind conditions to isolate how and when wind can lead to column collapse. We analyze these simulations via a novel method that provides stability percentage via an …

Theoretical Boundaries Of Annual Flood Risk For Single-Family Homes Within The 100-Year Floodplain, Ayat Al Assi, Rubayet Bin Mostafiz, Carol J. Friedland, Robert V. Rohli

Faculty Publications

Special flood hazard areas (SFHAs), defined as having an annual probability of occurrence of 1 percent or above, are used by U.S. Federal Emergency Management Agency (FEMA) to demarcate areas within which flood insurance purchase is required to secure a mortgage. However, quantifying flood risk within SFHAs can be challenging due to the lack of modeled flood depth data for all return periods. To address this issue, this research quantifies flood risk indicated by average annual loss (AAL) within the A Zone—the subset of the SFHA where wave heights can potentially range from 0 to 3 feet. The methodology resolves …

Cyber Attacks Against Industrial Control Systems, Adam Kardorff

LSU Master's Theses

Industrial Control Systems (ICS) are the foundation of our critical infrastructure, and allow for the manufacturing of the products we need. These systems monitor and control power plants, water treatment plants, manufacturing plants, and much more. The security of these systems is crucial to our everyday lives and to the safety of those working with ICS. In this thesis we examined how an attacker can take control of these systems using a power plant simulator in the Applied Cybersecurity Lab at LSU. Running experiments on a live environment can be costly and dangerous, so using a simulated environment is the …

The Politics Of Non-State Provisioning In The Middle East And North Africa, E Celeste Cavell

Honors Capstones

No abstract provided.

Development Of A 3d-Printed Device To Study How Bacteria Alters The 3d Migration Of Breast Cancer Cells, Jack Bedell

Honors Capstones

No abstract provided.

Electrochemical Reduction Of Co2: A Common Acetyl Path To Ethylene, Ethanol Or Acetate, Monsuru Dauda, John Hendershot, Mustapha Bello, Junghyun Park, Alvaro Loaiza Orduz, Nicholas Lombardo, Orhan Kizilkaya, Phillip Sprunger, Anthony Engler, Craig Plaisance, John Flake

Faculty Publications

Ethylene is well known as the primary product of CO2 reduction at Cu electrocatalysts using zero-gap membrane electrode assembly cells with gas diffusion cathodes. Other types of Cu electrocatalysts including oxide-derived Cu, CuSn and CuSe yield relatively more C2 oxygenates; however, the mechanisms for C2 product selectivity are not well established. This work considers selectivity trends of Cu-P0.065, Cu-Sn0.03, and Cu2Se electrocatalysts made using a standard one pot synthesis method. Results show that Cu-P0.065 electrocatalysts (Cuδ+ = 0.13) retain ethylene as a primary product with relatively higher Faradaic efficiencies (FE = 43% at 350 mA cm−2) than undoped Cu electrocatalysts …

Interactive Effects Of Co2, Temperature, And Nitrate Limitation On The Growth And Physiology Of Marine Cyanobacterium Synechococcus Sp. Ccmp 1334, Alyssa K. Sharbaugh

LSU Master's Theses

The marine cyanobacterium Synechococcus sp. CCMP 1334 was grown in a continuous culture system on a 12:12 h light:dark cycle at all combinations of low and high pCO2 (400 and 1000 ppmv, respectively), nitrate availability (nitrate-limited and nutrient-replete conditions), and temperatures of 21°C, 24°C, 28°C, 32°C, and 35°C. The maximum median nutrient-replete growth rate was ~1.15 d−1 at 32 –35°C. Median growth rates at 1000 ppmv pCO2 were higher than those at 400 ppmv at all temperatures, but most of the differences were statistically insignificant. Carbon:nitrogen ratios were independent of pCO2 at a fixed relative growth rate but decreased with …

Supply And Distribution Of Flow And Sediment In River And Delta System, Kazi M. Sadid

LSU Doctoral Dissertations

River deltas are valuable ecosystems that have been essential in supporting human populations and the economy across the world for centuries. However, climate change and human activities have caused significant land loss in delta areas. A prime example of a region that has suffered severe land loss is Louisiana in the United States. Coastal restoration is therefore essential for the livelihoods of its residents. To address this issue, the Lower Mississippi River (LMR) channel bar has been regularly dredged to rebuild degraded marsh areas. Additionally, sediment diversions off LMR have been designed to build marsh areas sustainably. This dissertation investigates …

Gut Microbiota And Blood Metabolites Related To Fiber Intake And Type 2 Diabetes, Zheng Wang, Brandilyn A. Peters, Bing Yu, Megan L. Grove, Tao Wang, Xiaonan Xue, Bharat Thyagarajan, Martha L. Daviglus, Eric Boerwinkle, Gang Hu, Yasmin Mossavar-Rahmani, Carmen R. Isasi, Rob Knight, Robert D. Burk, Robert C. Kaplan, Qibin Qi

Population and Public Health Science Faculty Publications

BACKGROUND: Consistent evidence suggests diabetes-protective effects of dietary fiber intake. However, the underlying mechanisms, particularly the role of gut microbiota and host circulating metabolites, are not fully understood. We aimed to investigate gut microbiota and circulating metabolites associated with dietary fiber intake and their relationships with type 2 diabetes (T2D). METHODS: This study included up to 11 394 participants from the HCHS/SOL (Hispanic Community Health Study/Study of Latinos). Diet was assessed with two 24-hour dietary recalls at baseline. We examined associations of dietary fiber intake with gut microbiome measured by shotgun metagenomics (350 species/85 genera and 1958 enzymes; n=2992 at …

The Power Of Social Capital: A Case Study Of The Louisiana State University Agcenter’S Agricultural Leadership Development Program, Andrew Schade

LSU Master's Theses

The Louisiana State University Agricultural Center’s Agricultural Leadership Development Program (LSU AgCenter ALDP) is a two-year nonformal education program for participants connected to various aspects of the agricultural and natural resources sectors. The purpose of the study was to describe the perspectives of graduates of the LSU AgCenter ALDP since 2005 regarding their self-reported outcomes after completing the program. The study used a qualitative, instrumental case study design, based on 12 semi-structured interviews, observation, and document analysis. The study employed adult learning theory and social capital theory as the theoretical frameworks to understand the impact of the program on participants. …

Role Of Electronegativity In Environmentally Persistent Free Radicals (Epfrs) Formation On Zno, Syed Monjur Ahmed, Reuben A. Oumnov, Orhan Kizilkaya, Randall W. Hall, Phillip T. Sprunger, Robert L. Cook

Faculty Publications

Environmentally persistent free radicals (EPFRs), a group of emerging pollutants, have significantly longer lifetimes than typical free radicals. EPFRs form by the adsorption of organic precursors on a transition metal oxide (TMO) surface involving electron charge transfer between the organic and TMO. In this paper, dihalogenated benzenes were incorporated to study the role of electronegativity in the electron transfer process to obtain a fundamental knowledge of EPFR formation mechanism on ZnO. Upon chemisorption on ZnO nanoparticles at 250 °C, electron paramagnetic resonance (EPR) confirms the formation of oxygen adjacent carbon-centered organic free radicals with concentrations between 10¹⁶ and 10 …

17O-Excess In Tropical Cyclones Reflects Local Rain Re-Evaporation More Than Moisture Source Conditions, Chijun Sun, Timothy Shanahan, Shaoneng He, Adriana Bailey, Jesse Nusbaumer, Jun Hu, Aubrey Hillman, Erika Ornouski, Jacob Warner, Kristine Delong

Faculty Publications

17O-excess is a relatively new water isotope parameter that could potentially provide useful information about the hydrological cycle. Previous works focusing on 17O-excess in polar regions suggest that it primarily tracks moisture source relative humidity, but little is known about how to interpret 17O-excess data in lower latitudes. Here we present quasi-hourly triple oxygen isotope data of precipitation collected from two tropical cyclones in Texas and Louisiana in 2020 to understand the impacts of environmental and meteorological processes on the 17O-excess of low-to mid-latitude precipitation. We find that at both hourly timescales and the event scale, 17O-excess is strongly correlated …

An Analysis And Ontology Of Teaching Methods In Cybersecurity Education, Sarah Buckley

LSU Master's Theses

The growing cybersecurity workforce gap underscores the urgent need to address deficiencies in cybersecurity education: the current education system is not producing competent cybersecurity professionals, and current efforts are not informing the non-technical general public of basic cybersecurity practices. We argue that this gap is compounded by a fundamental disconnect between cybersecurity education literature and established education theory. Our research addresses this issue by examining the alignment of cybersecurity education literature concerning educational methods and tools with education literature.

In our research, we endeavor to bridge this gap by critically analyzing the alignment of cybersecurity education literature with education theory. …

Mentorship Within Developmental Networks: A Phenomenological Study Of Black Women Tenured Associate Professors And Their Path Toward Full Professor, Jenifer F. Godfrey

LSU Doctoral Dissertations

The problem of inadequate representation of Black women in tenure track faculty positions is both pronounced and longstanding. With Black women making up less than 2% of full professors, this is particularly true at institutions categorized as R1 – Doctoral Universities – Very high research activity by The Carnegie Classification of Institutions of Higher Education. College faculty have long been the focus of scholarly research. However, studies focused on mentorship to be promoted to full professor for associate professors generally, and Black women associate professors specifically, are lacking. Using a conceptual framework that defined mentorship broadly as developmental networks and …

Her Seat At The Table: A Phenomenological Study Exploring The Lived Experiences Of Black Women Education Policymakers, Vickey R. Silas

LSU Doctoral Dissertations

ABSTRACT

This transcendental phenomenological study explored the lived experiences of Black women leaders as education policymakers to understand the essence of their journey to leadership positions and the impetus behind their decision-making. Despite progress, Black women remain marginalized and underrepresented in policymaking leadership roles and decision-making spaces (Griffin et. al, 2013). They face challenges that are intersectional like gender and race. My research question was what are the lived experiences of Black women education policymakers and how do these experiences impact their decision-making? While employing a transcendental phenomenological approach, I acknowledged my biases through reflexivity and bracketed them while also …