Digital Commons Network^™

Edwardsiella Ictaluri T3ss Effector Esen Modulates Expression Of Host Genes Involved In The Immune Response, Lidiya P. Dubytska, Ranjan Koirala, Azhia Sanchez, Ronald Thune

Faculty Publications

The type III secretion system (T3SS) effector EseN is encoded on the Edwardsiella ictaluri chromosome and is homologous to a family of T3SS effector proteins with phosphothreonine lyase activity. Previously we demonstrated that E. ictaluri invasion activates extracellular signal-regulated kinases 1 and 2 (ERK1/2) early in the infection, which are subsequently inactivated by EseN. Com-parative transcriptomic analysis showed a total of 753 significant differentially expressed genes in head-kidney-derived macrophages (HKDM) infected with an EseN mutant (∆EseN) compared to HKDM infected with wild-type (WT) strains. This data strongly indicates classical activation of macrophages (the M1 phenotype) in response to E. ictaluri …

Conditional Quantum Plasmonic Sensing, Fatemeh Mostafavi, Zeinab Jafari, Michelle L.J. Lollie, Chenglong You, Israel De Leon, Omar S. Magaña-Loaiza

Faculty Publications

The possibility of using weak optical signals to perform sensing of delicate samples constitutes one of the main goals of quantum photonic sensing. Furthermore, the nanoscale confinement of electromagnetic near fields in photonic platforms through surface plasmon polaritons has motivated the development of highly sensitive quantum plasmonic sensors. Despite the enormous potential of plasmonic platforms for sensing, this class of sensors is ultimately limited by the quantum statistical fluctuations of surface plasmons. Indeed, the fluctuations of the electromagnetic field severely limit the performance of quantum plasmonic sensing platforms in which delicate samples are characterized using weak near-field signals. Furthermore, the …

Levels And Correlates Of Objectively Measured Sedentary Behavior In Young Children: Sunrise Study Results From 19 Countries, Katharina E. Kariippanon, Kar A.R.H.A.U. Chong, Xanne Janssen, Simone A. Tomaz, Evelyn H.C. Ribeiro, Nyaradzai Munambah, Cecilia H.S. Chan, Pw Prasad Chathurangana, Catherine E. Draper, Asmaa El Hamdouchi, Alex A. Florindo, Hongyan Guan, Amy M.Y.S. Ha, Mohammad Sorowar Hossain, Dong Hoon Kim, Thanh Van Kim, Denise C.L. Koh, Marie Löf, Bang Nguyen Pham, Bee E.E.K. Poh, John J. Reilly, Amanda E. Staiano, Adang Suherman, Chiaki Tanaka, Hong K.I.M. Tang, Mark S. Tremblay, E. Kipling Webster, V. Pujitha Wickramasinghe, Jyh Y.H.E. Wong, Anthony D. Okely

Population and Public Health Science Faculty Publications

Purpose There is a paucity of global data on sedentary behavior during early childhood. The purpose of this study was to examine how device-measured sedentary behavior in young children differed across geographically, economically, and sociodemographically diverse populations, in an international sample. Methods This multinational, cross-sectional study included data from 1071 children 3-5 yr old from 19 countries, collected between 2018 and 2020 (pre-COVID). Sedentary behavior was measured for three consecutive days using activPAL accelerometers. Sedentary time, sedentary fragmentation, and seated transport duration were calculated. Linear mixed models were used to examine the differences in sedentary behavior variables between sex, country-level …

The Dual Nature Of The Nucleolus, Alan Tartakoff, Patrick Dimario, Eduard Hurt, Brian Mcstay, Vikram Govind Panse, David Tollervey

Faculty Publications

The nucleolus is best known for housing the highly ordered assembly line that produces ribosomal subunits. The >100 ribosome assembly factors in the nucleolus are thought to cycle between two states: an operative state (when integrated into subunit assembly intermediates) and a latent state (upon release from intermediates). Although it has become commonplace to refer to the nucleolus as “being a multilayered condensate,” and this may be accurate for latent factors, there is little reason to think that such assertions pertain to the operative state of assembly factors.

Ghost (Hi)Stories: Fiction As Alternative History In Brodber, Valdés, Cisneros, And Condé, Kristina Suzette Gibby

LSU Doctoral Dissertations

This dissertation analyzes the role that female ghosts play in recuperating memory and filling the gaps of official history in the following four contemporary novels: Erna Brodber’s Louisiana (1994), Zoé Valdés’s Te di la vida entera (1996), Sandra Cisneros’s Caramelo: or, Puro Cuento (2002), and Maryse Condé’s Victoire, les saveurs et les mots: récit (2006). The ghosts in these novels disrupt a linear temporality and present a matriarchal mode of remembering, leading readers to reconsider the past outside of the dominant historical discourse. In this way, the novels become alternative histories that oppose the monologic historical paradigm and recuperate marginalized …

Geographical Distribution, Allele Frequency, And Inheritance Of Cry1a.105 And Cry2ab2 Resistance In Helicoverpa Zea In The Southeastern Region Of The United States, Wenbo Yu

LSU Doctoral Dissertations

The corn earworm, Helicoverpa zea (Boddie), is a target pest of Bacillus thuringiensis (Bt) maize and cotton in the U.S. Previous studies have reported that this pest has developed field resistance to pyramided Cry1A/Cry2A maize and cotton in certain areas of the southern United States. The objectives of this study were to 1) determine the current status and distribution of the resistance to Cry1A.105 and Cry2Ab2 in H. zea in the southeastern United States; 2) estimate the resistance allele frequencies to the two Bt proteins in H. zea field populations in the region, and 3) assess the inheritance of the …

Amide-Based Electrolyte Battery, Daniel Kuroda, Kristen Fulfer, Kaylee Theresa Woodard

College of Science Patents

An energy storage apparatus is described and claimed herein comprising, generally, a battery housing enclosing a negative electrode, a positive electrode, and an electrolyte, wherein the electrolyte comprises a salt dissolved in either an amide-based solvent. In various embodiments, the amide-based solvent is a tertiary amide. Moreover, the energy storage apparatus may be a lithium ion battery that comprises an electrolyte with a lithium salt dissolved in a tertiary amide.

Readymades & Repertoires: Artifact-Mediated Improvisation In Tabletop Role-Playing Games, Philip Tchernavskij, Andrew M. Webb, Hayden Gemeinhardt, Wendy E. Mackay

Faculty Publications

Game masters (GMs) are creative practitioners who plan and orchestrate tabletop role-playing games. Through an interview study, we investigate how eight expert game masters adapt everyday technologies and materials as creativity support tools (CSTs) for improvisational and collaborative play. We integrate theories of improvisational and distributed creativity with the human-artifact model, which provides an activity-theoretical vocabulary for analyzing the mediating relationships between specialist practitioners and their tools. We show how GMs prepare and deploy readymade artifacts: analog and digital CSTs that flexibly mediate recurring creative tasks in their practice, such as improvising narrative elements, facilitating smooth play, and creating aesthetic …

Integration Of Solar + Storage Installation Upstream In Modular Construction, Ondrej Labik

LSU Master's Theses

High initial costs, mainly due to inefficient construction processes, remains the greatest barrier for residential adoption of solar+storage (SPS) system. Modular construction, having a factory-controlled environment and control over the home design, is well suited to address these issues (e.g., efficiencies, waste, inventory control, quality). Furthermore, these areas have great potential in modular construction to address the high initial cost issue. This study focuses on the integration of SPS installation upstream in modular housing. Although moving the SPS installation process into the factory has many barriers (e.g., including change of scope of work at some workstations without affecting the whole …

Bloch Spectra For High Contrast Elastic Media, Jayasinghage Ruchira Nirmali Perera

LSU Doctoral Dissertations

The primary goal of this dissertation is to develop analytic representation formulas and power series to describe the band structure inside periodic elastic crystals made from high contrast inclusions. We use source free modes associated with structural spectra to represent the solution operator of the Lame' system inside phononic crystals. Then we obtain convergent power series for the Bloch wave spectrum using the representation formulas. An explicit bound on the convergence radius is given through the structural spectra of the inclusion array and the Dirichlet spectra of the inclusions. Sufficient conditions for the separation of spectral branches of the dispersion …

The Nexus Of The Indoor Co2 Concentration And Ventilation Demands Underlying Co2-Based Demand-Controlled Ventilation In Commercial Buildings: A Critical Review, Xing Lu, Zhihong Pang, Yangyang Fu, Zheng O'Neill

Faculty Publications

The carbon dioxide (CO2)-based demand-controlled ventilation (DCV) has attracted prompt attention from the Heating, Ventilation, and Air-Conditioning (HVAC) industry since its very first invention. Since then, it has been penetrating from simple single-zone systems to complex system configurations in commercial buildings. While there has accumulated a large number of research on DCV applications, the most recent review paper on this topic was dated back to 2001 and inevitably missed a lot of recent revolutionary technologies. Therefore, to understand the opportunities and challenges associated with the CO2-based DCV, this study presents a timely review on the revolutions of the CO2-based DCV …

Hypothalamic Estrogen Signaling And Adipose Tissue Metabolism In Energy Homeostasis, Valeria C. Torres Irizarry, Yuwei Jiang, Yanlin He, Pingwen Xu

Division of Basic Science Faculty Publications

Obesity has become a global epidemic, and it is a major risk factor for other metabolic disorders such as type 2 diabetes and cardiometabolic disease. Accumulating evidence indicates that there is sex-specific metabolic protection and disease susceptibility. For instance, in both clinical and experimental studies, males are more likely to develop obesity, insulin resistance, and diabetes. In line with this, males tend to have more visceral white adipose tissue (WAT) and less brown adipose tissue (BAT) thermogenic activity, both leading to an increased incidence of metabolic disorders. This female-specific fat distribution is partially mediated by sex hormone estrogens. Specifically, hypothalamic …

Interindividual Variation In Maximum Aerobic Metabolism Varies With Gill Morphology And Myocardial Bioenergetics In Gulf Killifish, Bernard B. Rees, Jessica E. Reemeyer, Brian A. Irving

Faculty Publications

This study asked whether interindividual variation in maximum and standard aerobic metabolic rates of the Gulf killifish, Fundulus grandis, correlates with gill morphology and cardiac mitochondrial bioenergetics, traits reflecting critical steps in the O2 transport cascade from the environment to the tissues. Maximum metabolic rate (MMR) was positively related to body mass, total gill filament length and myocardial oxygen consumption during maximum oxidative phosphorylation (multiple R2=0.836). Standard metabolic rate (SMR) was positively related to body mass, total gill filament length and myocardial oxygen consumption during maximum electron transport system activity (multiple R2=0.717). After controlling for body mass, individuals with longer …

Regional Human Resource Development In Asean: An Institutional Theory Perspective, Oleksandr Tkachenko, Oliver S. Crocco, Loi A. Nguyen, Victoria Jonathan

Faculty Publications

With the growing influence of intergovernmental organizations such as the European Union and the Association of Southeast Asian Nations (ASEAN), the scope and complexity of human resource development (HRD) activities have expanded. Informed by an institutional theory perspective, we examined the evolution of HRD as well as HRD-related programs in ASEAN to illuminate the mechanisms and conditions shaping HRD in ASEAN. Our primary sources were archival data from ASEAN from 1967–2021 and relevant publications of international development agencies that feature HRD-related activities in the region. Our analysis suggests that the establishment of an intergovernmental organization (i.e., ASEAN), with its continuing …

Mitochondrial Uncoupling Attenuates Sarcopenic Obesity By Enhancing Skeletal Muscle Mitophagy And Quality Control, Wagner S. Dantas, Elizabeth R.M. Zunica, Elizabeth C. Heintz, Bolormaa Vandanmagsar, Z. Elizabeth Floyd, Yongmei Yu, Hisashi Fujioka, Charles L. Hoppel, Kathryn P. Belmont, Christopher L. Axelrod, John P. Kirwan

Division of Basic Science Faculty Publications

Background: Sarcopenic obesity is a highly prevalent disease with poor survival and ineffective medical interventions. Mitochondrial dysfunction is purported to be central in the pathogenesis of sarcopenic obesity by impairing both organelle biogenesis and quality control. We have previously identified that a mitochondrial-targeted furazano[3,4-b]pyrazine named BAM15 is orally available and selectively lowers respiratory coupling efficiency and protects against diet-induced obesity in mice. Here, we tested the hypothesis that mitochondrial uncoupling simultaneously attenuates loss of muscle function and weight gain in a mouse model of sarcopenic obesity. Methods: Eighty-week-old male C57BL/6J mice with obesity were randomized to 10 weeks of high …

Genetic Variation Among The Salinity Tolerant Breeding Lines Identified From Two Multi-Parent Advanced Generation Introgression Line Populations In Rice (Oryza Sativa), Sandeep Chapagain, Jonathan Concepcion, Rajat Pruthi, Lovepreet Singh, Adam Famoso, Prasant K. Subudhi

Faculty Publications

Salinity is a major environmental constraint affecting rice production in both irrigated and non-irrigated areas and threatening global food security. Development of salt-tolerant rice varieties with desirable agronomic traits is a logical approach to maintain high yield in saline areas. Here, we report development of two multi-parent advanced generation introgression line (MAGIL) rice populations in two US cultivar backgrounds and their physiological and morphological evaluation under salinity stress at germination and seedling stages. From the initial screening of 180 MAGILs in sand culture, 36 tolerant-to-moderately salt-tolerant lines were selected and further confirmed in hydroponics experiment. The selected MAGILs showed lower …

A Soft Syntactic Foam Actuator With High Recovery Stress, Actuation Strain, And Energy Output, Siavash Sarrafan, Xiaming Feng, Guoqiang Li

Faculty Publications

Novel syntactic foams were prepared by incorporating three types of glass microbubbles in a crosslinked cis poly(1,4-butadiene) (cPBD) matrix. The as-prepared semi-crystalline polymer-based syntactic foams displayed a clear two-way shape memory effect (2W-SME) with remarkable strain actuation of about 56% expansion when the temperature drops from 60 °C to − 40 °C, and about 40% contraction when the temperature rises from − 40 °C to 60 °C, comparable to most pure two-way shape memory polymers. Compared to the pure cPBD, which was actuated under 0.32 MPa tensile stress, the foams were actuated under a much larger external tensile stress of …

Co2 Zonal Injection Rate Allocation And Plume Extent Evaluation Through Wellbore Temperature Analysis, Refaat G. Hashish, Mehdi Zeidouni

Faculty Publications

Temperature analysis during a pause in injection operations, known as warmback analysis, has been used in the petroleum industry for evaluating the injection conformance and estimating the location of the flooded front in applications, such as waterflooding oil reservoirs. In this work, methods are introduced to extend the application of temperature warmback analysis to estimate the zonal CO2 injection rate and zonal CO2 plume extent during geologic CO2 storage in a saline aquifer. First, novel analytical solutions are developed to model transient temperature in the aquifer during the injection and subsequent shut-in periods considering two-phase flow (gaseous CO2 and aqueous …

Effects Of Bromocriptine On Glucose And Insulin Dynamics In Normal And Insulin Dysregulated Horses, Caroline M.M. Loos, Kristine L. Urschel, Eric S. Vanzant, Erin L. Oberhaus, Adam D. Bohannan, James L. Klotz, Kyle R. Mcleod

Faculty Publications

The objectives of the study were to study the effects of the synthetic ergot alkaloid (EA), bromocriptine, on glucose and lipid metabolism in insulin dysregulated (ID, n = 7) and non-ID (n = 8) mares. Horses were individually housed and fed timothy grass hay and two daily concentrate meals so that the total diet provided 120% of daily DE requirements for maintenance. All horses were given intramuscular bromocriptine injections (0.1 mg/kg BW) every 3 days for 14 days. Before and after 14 days of treatment horses underwent a combined glucose-insulin tolerance test (CGIT) to assess insulin sensitivity and a feed …

Whole-Genome Sequencing Of Kmr3 And Oryza Rufipogon-Derived Introgression Line Il50-13 (Chinsurah Nona 2/Gosaba 6) Identifies Candidate Genes For High Yield And Salinity Tolerance In Rice, Shashi Rekha Thummala, Haritha Guttikonda, Shrish Tiwari, Rajeshwari Ramanan, Niranjan Baisakh, Sarla Neelamraju, Satendra K. Mangrauthia

Faculty Publications

The genomes of an elite rice restorer line KMR3 (salinity-sensitive) and its salinity-tolerant introgression line IL50-13, a popular variety of coastal West Bengal, India, were sequenced. High-quality paired-end reads were obtained for KMR3 (147.6 million) and IL50-13 (131.4 million) with a sequencing coverage of 30X-39X. Scaffolds generated from the pre-assembled contigs of each sequenced genome were mapped separately onto the reference genome of Oryza sativa ssp. japonica cultivar Nipponbare to identify genomic variants in terms of SNPs and InDels. The SNPs and InDels identified for KMR3 and IL50-13 were then compared with each other to identify polymorphic SNPs and InDels …

Characterization Of Gas Hydrate Formation And Its Impact On Slope Stability In Offshore Environments, Sulav Dhakal

LSU Doctoral Dissertations

Natural gas hydrates (NGH) are predominantly methane hydrates formed at high pressure and low temperature conditions typically in submarine sediments and permafrost. This dissertation is focused on the submarine methane hydrates formed by upward migration of free and dissolved gas. The methane gas stored within natural hydrates has more energy potential than all the combined oil and gas resources around the world. Numerical simulation of fluid flow, heat transport, and geomechanical stresses is used to characterize and evaluate the saturation, distribution, reservoir quality, and geohazards associated with hydrate-bearing sediments. Coupled thermo-hydro-mechanical (THM) simulation of hydrate formation, dissociation, and slope stability …

Creating Musical Scores Inspired By The Intersection Of Human Speech And Music Through Model-Based Cross Synthesis, William Alexander Thompson Iv

LSU Doctoral Dissertations

This research addresses the development of machine learning techniques used to create musical scores and performances that are inspired by the intersection of speech and music. Machine learning models are created from MIDI files that are transcribed from datasets of musical audio recordings and human speech audio recordings. Through the creation of succinct models, model based cross synthesis is possible. Models trained on musical MIDI data are asked to replicate MIDI data that approximate human speech. Alternatively, models that have been trained on MIDI data that approximate speech are asked to replicate musical MIDI data. The product of these developed …

Lifecycle Reactive Transport Modeling For Assessing Integrity In Offshore Energy Systems, Temitope Ayokunle Ajayi

LSU Doctoral Dissertations

Subsurface leaks and spills are a matter of significant concern to the oil and gas industry because of their impact on health, safety and environment. It is thus important to study and assess the integrity of our energy systems and the physico-chemical processes that affect them.

To avoid leakage of fluids from wellbores, barrier materials are used for sealing formation fluids and creating a single flow path through the well. These barrier materials are constantly subjected to subsurface fluid fluxes and temperature, pressure and chemical conditions over the extended periods the wells remain underground. This makes it imperative to assess …

A Comparative Content Analysis Of How Print Media Framed The Heroin Epidemic And The Opioid Crisis, Quincy Hodges

LSU Doctoral Dissertations

This dissertation examined how the news media covered the Opioid Crisis of the 21st century compared to how the news media covered the Heroin Epidemic of the 1970s. A content analysis of six U.S. newspapers – the New York Times, the Washington Post, The Columbus Dispatch, The Detroit Free Press, The Atlanta Journal-Constitution, and the New Orleans Times-Picayune indicated that the print news media covered the Heroin Epidemic and the Opioid Crisis drastically different. The news media framed the Opioid Crisis as a public health issue, focusing coverage on rogue doctors and health professionals, and how unethical pharmaceutical companies took …

A New Insight Into Fungal Cell Wall Architecture By Functional Genomics And Solid-State Nmr Along With Recent Advancements In Dynamic Nuclear Polarization For Analyzing Biomolecules, Arnab Chakraborty

LSU Master's Theses

This dissertation summarizes the findings related to the way by which supramolecular architecture of fungal cell wall changes with genetic mutation, dispensing genes responsible for biosynthesis of cell wall polysaccharides. This is necessary because without perfect picture of how supramolecular assembly changes with genetic mutation it is hard to assess new anti-fungal targets. Alongside this we have highlighted how recent advancement into Dynamic Nuclear Polarization (DNP) methods improved characterization of biomolecules both in case of labeled and unlabeled samples.

First study utilized Solid-state NMR (SSNMR) which is a non-destructive technique hence enabled us for the first time to deduce how …

The Impact Of Second-Year Membership Experiences On Retention Among Panhellenic Sororities, Elizabeth Richardson Newell

LSU Doctoral Dissertations

ABSTRACT

Sorority membership retention is a newly emerging research topic with little to no existing information on what motivates members to remain. There are countless studies concerning college student retention in general, but there have yet to be studies that focus specifically on sorority women and their membership experiences. One major research question drove this study: What factors and/or experiences influence a second year National Panhellenic Conference (NPC) sorority member’s decision to actively participate in their sorority?

Using a theoretical framework developed from the social integration retention model proposed by Braxton, Hirschy, and McClendon in 2011, this study explored what …

Unifying Text And Music: Applying Shurtleff's Twelve Guideposts To Operatic Arias For Soprano, Jamey J. Wright

LSU Doctoral Dissertations

In the present culture of operatic performance, artistry is measured not only by singers’ musical interpretation and vocal technique, but also by their ability to communicate compelling characterization. This monograph explores a helpful technique for dramatic interpretation in opera. The discipline of musical performance provides helpful guides to characterization through elements such phrasing, rhythm, harmony, and instrumentation. Techniques borrowed from the spoken theater, particularly those pioneered by Constantin Stanislavski, include the Magic If, objectives, actions, and tactics. The literature on auditioning for the theater is useful for synthesizing these techniques for singers, because audition techniques emphasize compelling characterization with …

Transitioning From Traditional In-Person Studio-Based Education Of Apparel Design Courses To Online Education During The Covid-19 Pandemic, Divya Desai

LSU Doctoral Dissertations

Due to the COVID-19 pandemic and subsequent lockdowns many post-secondary educational institutions all-around the world, including the USA, were forced to transition their teaching methods from a traditional in-person to an online teaching format starting in the spring of 2020. This research however is focused on the transitioning of university-level studio-based Apparel Design (AD) courses to an online teaching format during the spring and fall 2020 semesters. Teaching AD courses that are studio-based and that particularly require hands-on skills like hand rendering, draping, sewing, or pattern drafting, are challenging to be taught online as they require in person step-by-step guidance …

Grotesque Masculinities In The Works Of Harry Crews, Barry Hannah, And Padgett Powell, Matt Brandon Blasi

LSU Doctoral Dissertations

“Grotesque Masculinities in the Works of Harry Crews, Barry Hannah, and Padgett Powell” explores how these authors use the grotesque to complicate, distort, and criticize hegemonic white Southern masculinity as represented in contemporary American literature. In “Grotesque Masculinities,” I argue that the presence of the grotesque mode in these author’s works offers a unique critical perspective by which to better understand how masculinity is constructed by and for white Southern men in literature, and how alternative configurations of identity are not only possible, but necessary to decenter whiteness and heteronormativity as dominant categories. Using what sociologists refer to as body-reflexive …

The Prime Aesthetic: Exploratory Research On The Relationship Between The Imprinting Of Aesthetic Dress Preferences And The Sensitive Period Of Emerging Adulthood, Jeremy Bernardoni

LSU Doctoral Dissertations

The purpose of this research was to explore the theoretical concept of Prime Aesthetic across generational cohorts through the investigation of individual aesthetic dress preferences (ADP), which are formed during the Prime of Life, and their potential influence on present-day ADP. The Prime Aesthetic concept is defined as a theoretical concept representative of the cumulative ADP imprinted from all influences during an individual’s Prime of Life. The Prime Aesthetic was derived from Holbrook and Schindler’s (1989) study on the development of musical tastes during late adolescence and early adulthood and linked to theories on imprinting during sensitive periods (Bornstein, 1989) …