Digital Commons Network^™

Development Of The Cell-Free Protein Biomanufacturing Platform For The Therapeutic Human Protein Production And The Topical Delivery Applications Using The Nanosized Hydrogel System, Jeehye Kim

LSU Doctoral Dissertations

Proteins are highly structural polymers, performing an enormous range of roles within organisms. Unlike synthetic polymers, proteins have a significant hierarchical ordering that can be disrupted by many environmental factors, which limits the scale-up manufacturing capability as well as the utilization of proteins as therapeutic agents. In this study, the Cell-Free Protein Synthesis (CFPS) system was developed to enhance the production capacity and applications of the therapeutic proteins. The CFPS is an advanced biosynthetic and genetic-engineered system. It enables the efficient production of proteins when the conventional cell-based protein synthesis systems cannot be applied, and when sophisticated control is required …

Rapid Spectral Variability Of A Giant Flare From A Magnetar In Ngc 253, O. J. Roberts, P. Veres, M. G. Baring, M. S. Briggs, C. Kouveliotou, E. Bissaldi, G. Younes, S. I. Chastain, J. J. Delaunay, D. Huppenkothen, A. Tohuvavohu, P. N. Bhat, E. Göğüş, A. J. Van Der Horst, J. A. Kennea, D. Kocevski, J. D. Linford, S. Guiriec, R. Hamburg, C. A. Wilson-Hodge, E. Burns

Faculty Publications

Magnetars are neutron stars with extremely strong magnetic fields (1013 to 1015 gauss)1,2, which episodically emit X-ray bursts approximately 100 milliseconds long and with energies of 1040 to 1041 erg. Occasionally, they also produce extremely bright and energetic giant flares, which begin with a short (roughly 0.2 seconds), intense flash, followed by fainter, longer-lasting emission that is modulated by the spin period of the magnetar3,4 (typically 2 to 12 seconds). Over the past 40 years, only three such flares have been observed in our local group of galaxies3–6, and in all cases the extreme intensity of the flares caused the …

Ceo Political Ideology: Implications For Firm Innovation, Samira Fallah

LSU Doctoral Dissertations

In this dissertation, I examine the influence of CEO political ideology on firm’s innovation strategy and outcomes. In my theorizing, I draw on upper-echelons theory that underscores the role of executives’ personal orientations on their decisions as well as the scholarship in political psychology that demonstrates the different values and psychological needs of liberals and conservatives. Given liberals’ lower need to manage uncertainty and their openness to new experiences, I propose that a firm with a more liberal CEO is likely to develop more innovations, pursue exploratory innovations and develop innovations with extreme quality (high and low). I also build …

A Microscale Approach To Investigate Breast Cancer Cellular Communication And Migration, Sharif Mohammad Mizanur Rahman

LSU Doctoral Dissertations

Metastatic breast cancer significantly decreases patient survival, with the majority of deaths caused by secondary metastasis. Cancer metastasis is a multi-step process that starts with the detachment of a cancer cell from the primary tumor and ends with establishing a secondary tumor at a distal location. This work's focus was to use an interdisciplinary microscale approach to study how cancer cells respond to neighboring cells and how they migrate in response to chemical gradients. Two distinct devices, a microfluidic device, and a 3D-printed plate insert, were developed to perform co-culture studies to elucidate the impact of ASCs on cancer cell …

Small Molecule Inhibitors Of The Bacterioferritin (Bfrb)-Ferredoxin (Bfd) Complex Kill Biofilm-Embedded Pseudomonas Aeruginosa Cells, Anabel Soldano, Huili Yao, Achala N.D. Punchi Hewage, Kevin Meraz, Joel K. Annor-Gyamfi, Richard A. Bunce, Kevin P. Battaile, Scott Lovell, Mario Rivera

Faculty Publications

© 2020 American Chemical Society. Bacteria depend on a well-regulated iron homeostasis to survive adverse environments. A key component of the iron homeostasis machinery is the compartmentalization of Fe3+ in bacterioferritin and its subsequent mobilization as Fe2+ to satisfy metabolic requirements. In Pseudomonas aeruginosa Fe3+ is compartmentalized in bacterioferritin (BfrB), and its mobilization to the cytosol requires binding of a ferredoxin (Bfd) to reduce the stored Fe3+ and release the soluble Fe2+. Blocking the BfrB-Bfd complex in P. aeruginosa by deletion of the bfd gene triggers an irreversible accumulation of Fe3+ in BfrB, concomitant cytosolic iron deficiency and significant impairment …

Censorship Concerns In College Media: A Multiple Case Study Analysis On The Silencing Of Student Journalists, Haley Nicole Matlock

LSU Doctoral Dissertations

The aim of this qualitative study was to conduct a multiple case study that provides an analysis of censorship concerns at campus newspapers affiliated with public, four-year universities. Eighteen individuals from seven institutions participated in interviews. Interviewees consisted of former and current student journalists and advisers who worked at university publications where allegations of censorship have occurred within the last decade. The Student Press Law Center routinely investigates claims of censorship and provides pro bono legal counsel to student journalists (Zagier, 2011). While courts commonly sided with students in disagreements regarding free speech, Hazelwood v Kuhlmeier (1988) scaled back freedoms …

Are We There Yet? Educators As Lgbtq Advocates And Book Clubs As Professional Development, Alexis Marie Egan

LSU Doctoral Dissertations

This dissertation examines Rainbow Advocates: An Educator’s Book Club, a book club study designed for educators wherein they read three works of LGBTQ young adult literature in Summer 2020. Purposes of the book club study include a consideration of how participating educators’ experiences with LGBTQ youth, literary materials, and training compare to past research. This dissertation includes an assessment of their readiness to handle LGBTQ issues in school sites and an evaluation of book clubs and reading LGBTQ young adult literature as a method of professional development for educators. In doing so, this dissertation draws on reader responses as per …

Mutant Tp53 Interacts With Bcar1 To Contribute To Cancer Cell Invasion, Alvin Kunyao Guo, Yoko Itahana, Veerabrahma Pratap Seshachalam, Hui Ying Chow, Sujoy Ghosh, Koji Itahana

Division of Basic Science Faculty Publications

Background: Mutant TP53 interacts with other proteins to produce gain-of-function properties that contribute to cancer metastasis. However, the underlying mechanisms are still not fully understood. Methods: Using immunoprecipitation and proximity ligation assays, we evaluated breast cancer anti-estrogen resistance 1 (BCAR1) as a novel binding partner of TP53R273H, a TP53 mutant frequently found in human cancers. The biological functions of their binding were examined by the transwell invasion assay. Clinical outcome of patients was analysed based on TP53 status and BCAR1 expression using public database. Results: We discovered a novel interaction between TP53R273H and BCAR1. We found that BCAR1 translocates from …

Characterization Of Common Phytoplankton On The Louisiana Shelf, M L. Parsons, A L. Brandt, R. Eugene Turner, W L. Morrison, M L. Parsons

Faculty Publications

Phytoplankton and accompanying environmental data (temperature, salinity, secchi depth, stratification, and inorganic nutrients) were analyzed from 672 surface water samples (0 to 1.5 m depth) collected from 95 stations located on the Louisiana shelf between April 1990 and August 2011. Phytoplankton were identified to the lowest practical taxonomic unit from glutaraldehyde-preserved samples using epifluorescent microscopy and reported as cells L-1. Twenty-six phytoplankton taxa (primarily diatoms) that were 8 mu m in size, identified to genuslevel resolution and ranked in the top 20 in at least one of three separate categories (average abundance; frequency of occurrence; and bloom frequency) were used …

Early Diagenesis In The Hypoxic And Acidified Zone Of The Northern Gulf Of Mexico: Is Organic Matter Recycling In Sediments Disconnected From The Water Column?, C. Rabouille, B. Lansard, S.M. Owings, N.N. Rabalais, B. Bombled, E. Metzger, J. Richirt, E.M. Eitel, A.D. Boever, J.S. Beckler, M. Taillefert

Faculty Publications

No abstract provided.

Lsu General Catalog 2021-2022, Louisiana State University And Agricultural And Mechanical College

LSU General Catalog

The LSU General Catalog describes all undergraduate and graduate departments and programs with degree requirements and courses offered for each one. The LSU General Catalog includes information on registration and financial aid as well as academic services offered to all students.

A Study Of The Effects Of The Covid-19 Pandemic On College Student Academic Dishonesty, Drake Fromenthal

Honors Capstones

No abstract provided.

Louisiana Agriculture Winter, 2021, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

Absorption Spectroscopy Of Quantum Black Holes With Gravitational Waves, Ivan Agullo, Vitor Cardoso, Adrián Del Rio, Michele Maggiore, Jorge Pullin

Faculty Publications

The observation of electromagnetic radiation emitted or absorbed by matter was instrumental in revealing the quantum properties of atoms and molecules in the early XX century, and constituted a turning-point in the development of the quantum theory. Quantum mechanics changes dramatically the way radiation and matter interact, making the probability of emission and absorption of light strongly frequency dependent, as clearly manifested in atomic spectra. In this essay, we advocate that gravitational radiation can play, for the quantum aspects of black holes, a similar role as electromagnetic radiation did for atoms, and that the advent of gravitational-wave astronomy can bring …

Anomalies In The Cosmic Microwave Background And Their Non-Gaussian Origin In Loop Quantum Cosmology, I Agullo, D Kranas, V Sreenath

Faculty Publications

Anomalies in the cosmic microwave background (CMB) refer to features that have been observed, mostly at large angular scales, and which show some tension with the statistical predictions of the standard ?CDM model. In this work, we focus our attention on power suppression, dipolar modulation, a preference for odd parity, and the tension in the lensing parameter A ( L ). Though the statistical significance of each individual anomaly is inconclusive, collectively they are significant, and could indicate new physics beyond the ?CDM model. In this article, we present a brief, but pedagogical introduction to CMB anomalies and propose a …

Spitzer's Last Look At Extragalactic Explosions: Long-Term Evolution Of Interacting Supernovae, T Szalai, O D. Fox, R G. Arendt, E Dwek, T Szalai, G C. Clayton, A V. Filippenko, J Johansson, P L. Kelly, K Krafton, A P. Marston, J C. Mauerhan, D Y. Van, Sd

Faculty Publications

Here we present new, yet final, mid-infrared (mid-IR) data for supernovae (SNe) based on measurements with the Spitzer Space Telescope. Comparing our recent 3.6 and 4.5 mu m photometry with previously published mid-IR and further multiwavelength data sets, we were able to draw some conclusions about the origin and heating mechanism of the dust in these SNe or in their environments, as well as about possible connection with circumstellar matter (CSM) originating from pre-explosion mass-loss events in the progenitor stars. We also present new results regarding both certain SN classes and single objects. We highlight the mid-IR homogeneity of SNe …

Gaia Gral: Gaia Dr2 Gravitational Lens Systems. Vi. Spectroscopic Confirmation And Modeling Of Quadruply Imaged Lensed Quasars, D Stern, S G. Djorgovski, A Krone-Martins, D Sluse, D Stern, C Ducourant, R Teixeira, J Surdej, C Boehm, B R. Den, D O. J, D R. D, G A. A, G R. L, J A. Mj, K L. P, L L. J, J F. Campion, A Mahabal, F Mignard, T Murphy, A Nierenberg, S Scarano, J Simon, J Slezak, C Spindola-Duarte, J Wambsganss

Faculty Publications

Combining the exquisite angular resolution of Gaia with optical light curves and WISE photometry, the Gaia Gravitational Lenses group (GraL) uses machine-learning techniques to identify candidate strongly lensed quasars, and has confirmed over two dozen new strongly lensed quasars from the Gaia Data Release 2. This paper reports on the 12 quadruply imaged quasars identified by this effort to date, which is a similar to 20% increase in the total number of confirmed quadruply imaged quasars. We discuss the candidate selection, spectroscopic follow-up, and lens modeling. We also report our spectroscopic failures as an aid for future investigations.

The Stormy Present: Conservatism And The Problem Of Slavery In Northern Politics, Christian R. Esh

Civil War Book Review

In The Stormy Present, Adam I. P. Smith argues antebellum northerners shared a conservative "disposition" that compelled them to defend the Union "bequeathed to them by the Founding" from the threats of a radical southern Slave Power.

Biology And Management Of The Rice Stink Bug (Hemiptera: Pentatomidae) In Rice, Oryza Sativa (Poales: Poaceae), Santhi Bhavanam, Blake Wilson, Bryce Blackman, Michael Stout

Faculty Publications

The rice stink bug, Oebalus pugnax (Fabricius), is the most important late-season insect pest of rice in the southern United States. Nymphs and adults feed on rice grains, resulting in losses in yield and grain quality. Aspects of the biology, ecology, life history, and chemical ecology have been investigated in southern rice producing states but several outstanding questions remain. Standard economic threshold levels (ETLs) based on sweep net sampling for rice stink bug have been established depending on grain development stage and geographic location. However, recent studies on the relationship between rice stink bug densities, feeding damage, and its economic …

Dominance And Fitness Costs Of Insect Resistance To Genetically Modified Bacillus Thuringiensis Crops, Fangneng Huang

Faculty Publications

Evolution of resistance to genetically modified Bacillus thuringiensis (Bt) crops in pest populations is a major threat to the sustainability of the technology. Incidents of field resistance that have led to control problems of Bt crops or significantly reduced susceptibility of individual Bt proteins in pyramided plants have increased dramatically across the world, especially in recent years. Analysis of globally published data showed that 61.5% and 60.0% of the cases of resistance with major alleles that allowed homozygous resistant genotypes to survival on Bt crops were functionally non-recessive and did not involve fitness costs, respectively. Dominance levels (DFLs) measured on …

S21rs Sgcr No. 25 (Black Caucus Constitution), Javin Bowman

Student Senate Enrolled Legislation

A Concurrent Resolution

To establish the LSU Student Government Black Caucus

S21rs Sgcr No. 33 (Constitutional Referendum), Alexandra Basse

Student Senate Enrolled Legislation

A Concurrent Resolution

To place the 2021 Constitution on the Spring 2021 Ballot

Matchless Organization: The Confederate Army Medical Department, Dale C. Smith

Civil War Book Review

Hasegawa shows that phenomena endemic to the Confederacy’s government and culture—namely material want and overwrought independence—hampered attempts to systematize and rationalize the Confederate Army’s medical department. Reviewer Dale C. Smith believes “anyone with a serious interest in the War of the Rebellion medicine should read it.”

Distinguishing Relational Turbulence, Marital Satisfaction, And Parenting Stress As Predictors Of Ineffective Arguing Among Parents Of Children With Autism, Kellie St Cyr Brisini, Denise Haunani Solomon

Faculty Publications

Relational turbulence theory suggests that relational turbulence causes pervasive patterns of dysfunctional communication in romantic relationships, such as an inability to resolve conflicts. Other research traditions link marital conflict to marital satisfaction and also exposure to stress. This study assesses the discriminant validity of relational turbulence by examining its association with reports of ineffective arguing, above and beyond the associations with marital satisfaction and parenting stress. The study tests the theoretical claims within the high stress context of parents raising children with autism spectrum disorder (ASD). Married, parents of children with ASD (N = 298) completed measures of parenting stress …

Siah2 Is Expressed In Adipocyte Precursor Cells And Interacts With Ebf1 And Zfp521 To Promote Adipogenesis, Thanh N. Dang, Jessica L. Taylor, Gail Kilroy, Yongmei Yu, David H. Burk, Z. Elizabeth Floyd

Division of Basic Science Faculty Publications

Objective: Expression of zinc finger protein 423 (ZFP423), a key proadipogenic transcription factor in adipocyte precursor cells, is regulated by interaction of the proadipogenic early B-cell factor 1 (EBF1) and antiadipogenic ZFP521. The ubiquitin ligase seven-in-absentia homolog 2 (SIAH2) targets ZFP521 for degradation. This study asked whether SIAH2 is expressed in adipocyte precursor cells and whether SIAH2 interacts with ZFP521 and EBF1 to regulate ZFP521 protein levels during adipogenesis. Methods: SIAH2 expression in precursor cells was assessed in primary cells and tissues from wild-type and SIAH2 null mice fed a control or high-fat diet. Primary cells, 3T3-L1 preadipocytes, and HEK293T …

Effects Of Caloric Restriction On Human Physiological, Psychological, And Behavioral Outcomes: Highlights From Calerie Phase 2, James L. Dorling, Stephan Van Vliet, Kim M. Huffman, William E. Kraus, Manjushri Bhapkar, Carl F. Pieper, Tiffany Stewart, Sai Krupa Das, Susan B. Racette, Susan B. Roberts, Eric Ravussin, Leanne M. Redman, Corby K. Martin

Clinical Research Faculty Publications

Caloric restriction (CR) is a strategy that attenuates aging in multiple nonhuman species. The Comprehensive Assessment of Long-term Effects of Reducing Intake of Energy (CALERIE) trials are part of a research program aiming to test the effects of CR on aging and longevity biomarkers in humans. Building on CALERIE phase 1, CALERIE phase 2 (CALERIE 2) was the largest study to date to assess sustained CR in healthy humans without obesity. In a 24-month randomized controlled trial comprising 218 participants at baseline, CALERIE 2 showed that moderate CR, 11.9% on average, induced improvements in aging-related biomarkers without adversely affecting psychological …

Constrained Learning Of Task-Related And Spatially-Coherent Dictionaries From Task Fmri Data, Sreekrishna Ramakrishnapillai, Harris R. Lieberman, Jennifer C. Rood, Stefan M. Pasiakos, Kori Murray, Preetham Shankapal, Owen T. Carmichael

Clinical Research Faculty Publications

Dictionary learning and sparse coding techniques overcome limitations of traditional voxel-level analyses of task-based functional magnetic resonance imaging (fMRI) data by identifying broader temporal and spatial patterns of brain activity. However, prior applications of these methods to task-related fMRI data are not simultaneously optimized to find temporal patterns of activity that change in concert with changes in task conditions and spatial patterns that leverage existing neuroscience knowledge. In this study we present a new sparse dictionary learning method that uses prior knowledge of the temporal pattern of task conditions and the locations of brain regions hypothesized to be involved in …

Christian Nationalism And Views Of Immigrants In The United States: Is The Relationship Stronger For The Religiously Inactive?, Samuel Stroope, Heather M. Rackin, Paul Froese

Faculty Publications

Previous research has shown that Christian nationalism is linked to nativism and immigrant animus, while religious service attendance is associated with pro-immigrant views. The findings highlight the importance of distinguishing between religious ideologies and practices when considering how religion affects politics. Using a national sample of U.S. adults, we analyze immigrant views by measuring levels of agreement or disagreement that undocumented immigrants from Mexico are “mostly dangerous criminals.” We find that Christian nationalism is inversely related to pro-immigrant views for both the religiously active and inactive. However, strongly pro-immigrant views are less likely and anti-immigrant views are more likely among …

Eat Clean And Safe Food: A Food-Based Dietary Guideline For The Elderly In South Africa, Makenzie Miller, Wilna Oldewage-Theron, Carin Napier

Faculty Publications

As the population of elderly individuals in South Africa (SA) grows, there is a need to promote the continued health of these persons as they progress through the life cycle. Food Based Dietary Guidelines (FBDGs) for the SA elderly were developed to address this need. These thirteen guidelines for the elderly collectively offer a basis of health practices that the elderly can follow to ensure that they are taking the right steps toward maintaining their health. While the guideline ‘Eat clean and safe food’ is not included in the current SA FBDGs, this recommendation is of particular importance to the …

Solopreneur Digital Ecosystems: Genesis, Lineage And Preliminary Categorization, Gabriele Piccoli, Biagio Palese, Joaquin Rodriguez

Faculty Publications

This paper traces the genesis and lineage of solopreneur digital ecosystems. These ecosystems, fostered by a digital environment that is infrastructural, combinatorial and servitized, are enabling the rise to prominence of the solopreneur. We theorize solopreneur digital ecosystems as the latest incarnation of systems beyond firm control, with digital platforms and digital marketplaces as their principal enablers. In an effort to compare them from the perspective of the solopreneur, we categorize solopreneur digital ecosystems on three dimensions: algorithmic control, commoditization, and lock-in. Our work contributes a framework that solopreneurs can use to identify ecosystems in which they can optimally invest …