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Genome-Wide Analysis Of The Human Alu Yb-Lineage., Anthony B. Carter, Abdel Halim Salem, Dale J. Hedges, Catherine Nguyen Keegan, Beth Kimball, Jerilyn A. Walker, W. Scott Watkins, Lynn B. Jorde, Mark A. Batzer Jan 2004

Genome-Wide Analysis Of The Human Alu Yb-Lineage., Anthony B. Carter, Abdel Halim Salem, Dale J. Hedges, Catherine Nguyen Keegan, Beth Kimball, Jerilyn A. Walker, W. Scott Watkins, Lynn B. Jorde, Mark A. Batzer

Faculty Publications

The Alu Yb-lineage is a 'young' primarily human-specific group of short interspersed element (SINE) subfamilies that have integrated throughout the human genome. In this study, we have computationally screened the draft sequence of the human genome for Alu Yb-lineage subfamily members present on autosomal chromosomes. A total of 1,733 Yb Alu subfamily members have integrated into human autosomes. The average ages of Yb-lineage subfamilies, Yb7, Yb8 and Yb9, are estimated as 4.81, 2.39 and 2.32 million years, respectively. In order to determine the contribution of the Alu Yb-lineage to human genomic diversity, 1,202 loci were analysed using polymerase chain reaction …

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Negotiating The Interregnum The Political Works Of Davenant And Tatham, Chad Thomas Jan 2004

Negotiating The Interregnum The Political Works Of Davenant And Tatham, Chad Thomas

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

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Feeding And Maturation By Soybean Looper (Lepidoptera: Noctuidae) Larvae On Soybean Affected By Weed, Fungus, And Nematode Pests, Carol H. Carter-Wientjes, John S. Russin, David J. Boethel, James L. Griffin, Edward C. Mcgawley Jan 2004

Feeding And Maturation By Soybean Looper (Lepidoptera: Noctuidae) Larvae On Soybean Affected By Weed, Fungus, And Nematode Pests, Carol H. Carter-Wientjes, John S. Russin, David J. Boethel, James L. Griffin, Edward C. Mcgawley

Faculty Publications

Feeding and maturation by the soybean looper, Pseudoplusia includens (Walker) (Lepidoptera: Noctuidae), were investigated in a 2-yr study on 'Davis' soybean, Glycine max (L.) Merr., grown alone and combined with the weed hemp sesbania, Sesbania exaltata (Raf.) Rybd. ex. A. W. Hill, the root-knot nematode, Meloidogyne incognita (Kofoid & White) Chitwood, and the charcoal rot fungus, Macrophomina phaseolina (Tassi) Goid. Of the three pests, hemp sesbania had the greatest effects on plant growth and insect feeding and maturation. When fed foliage from soybean stressed by hemp sesbania, soybean looper larvae remained longer in feeding stages, consumed more foliage, and showed …

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Site-Directed Mutagenesis Studies Of E. Coli Biotin Carboxylase, Valerie Melissa Sloane Jan 2004

Site-Directed Mutagenesis Studies Of E. Coli Biotin Carboxylase, Valerie Melissa Sloane

LSU Doctoral Dissertations

Acetyl-CoA carboxylase is an essential enzyme in all plants, animals, and bacterie, where it catalyzes the committed step in fatty acid synthesis. The Escherichia coli version of the enzyme has three functional components that dissociate readily: biotin carboxylase, carboxyltransferase, and a biotin carboxyl carrier protein. The biotin carboxylase component catalyzes the ATP-dependent carboxylation of biotin, using bicarbonate as the carboxylate source. Biotin carboxylase is a member of a functionally diverse superfamily of proteins known as the ATP-grasp enzymes. In the first study, four residues of biotin carboxylase, Lys 116, Lys 159, His 209, and Glu 276, were selected for site-directed …

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Against Biopoetics: On The Use And Misuse Of The Concept Of Evolution In Contemporary Literary Theory, Bradley Bankston Jan 2004

Against Biopoetics: On The Use And Misuse Of The Concept Of Evolution In Contemporary Literary Theory, Bradley Bankston

LSU Doctoral Dissertations

This dissertation is a critical assessment of "biopoetics:" a new literary theory that attempts to import ideas from evolutionary science to the study of literature. Borrowing from the field of evolutionary psychology, the biopoeticists argue that some literary forms and themes are particularly valuable because they result from our innate and evolved cognitive structure; they also attempt to create a normative aesthetic from the idea that evolution is progressive. In its first half, this study examines the claims of evolutionary psychology and their application by the biopoeticists; in the second half, it examines the idea that evolution is progressive, and …

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The Influence Of State Merit-Based Aid On Access And Educational Experiences: An Exploration Of The Louisiana Tuition Opportunity Program For Students (Tops), Roderick L. Smothers Jan 2004

The Influence Of State Merit-Based Aid On Access And Educational Experiences: An Exploration Of The Louisiana Tuition Opportunity Program For Students (Tops), Roderick L. Smothers

LSU Doctoral Dissertations

Access to higher education at the beginning of the new millennium faces unprecedented challenges. Although many of those challenges have been identified and addressed through research and federal and state policies, there still exists an inherent gap in the current and potential future success of today’s college students. This is particularly true for minority and lower-income students. Some of the identified challenges include: lack of financial assistance, persisting through and graduating from college, pre-college preparation, socioeconomic status, and family support/motivation. This study focuses primarily on the financial barriers that impede the process of providing equal access to all students, which …

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Mothering Modes: Analyzing Mother Roles In Novels By Twentieth-Century United States Women Writers, Preselfannie Whitfield Mcdaniels Jan 2004

Mothering Modes: Analyzing Mother Roles In Novels By Twentieth-Century United States Women Writers, Preselfannie Whitfield Mcdaniels

LSU Doctoral Dissertations

For this dissertation, the following novels have been chosen as examples of the many issues that are involved in mothering in United States society: Chapter 1: Dorothy Allison’s Bastard Out of Carolina and Toni Morrison’s Beloved, Chapter 2: Toni Morrison’s Song of Solomon and Dorothy West’s The Wedding, Chapter 3: Amy Tan’s The Kitchen God’s Wife and Christina García’s Dreaming in Cuban, and Chapter 4: Betty Smith’s A Tree Grows in Brooklyn and Paule Marshall’s Brown Girl, Brownstones. For this study, the term “mothering” is specifically related to the rearing of children by the female parent. Rearing is defined as …

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Soils Of Regeneration: Exploring Conceptualizations Of The Natural World As A Context For An Ecologically-Sensitive Curriculum, Emily A. Demoor Jan 2004

Soils Of Regeneration: Exploring Conceptualizations Of The Natural World As A Context For An Ecologically-Sensitive Curriculum, Emily A. Demoor

LSU Doctoral Dissertations

David Orr (1994) asserts that the ecological crisis is a crisis of education. This study explores the relationship between the ecological crisis and education by examining the role that language plays in shaping perceptions of the natural world. Toward this end it analyzes narratives of science, literature and other disciplines that conceptualize the natural world as object and as subject. It evaluates how particular metaphors used in reference to the natural world enhance or impede ecological understanding and the cultivation of responsibility and stewardship and considers ways in which these conceptualizations might be used as a basis for new curriculum …

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Leptin In Horses: Influences Of Body Condition, Gender, Insulin Insensitivity, Feeding, And Dexamethasone, Joshua Allen Cartmill Jan 2004

Leptin In Horses: Influences Of Body Condition, Gender, Insulin Insensitivity, Feeding, And Dexamethasone, Joshua Allen Cartmill

LSU Doctoral Dissertations

The experiments described herein were designed to answer questions that arose from initially attempting to determine whether treatment with dexamethasone increased concentrations of leptin in geldings. Dexamethasone treatment did in fact increase leptin concentrations in mares, geldings, and stallions. Additional experiments were designed to determine leptin's interaction, not only with the adrenal axis, but with the thyroid axis, the growth hormone/insulin-like growth factor-1 axis, as well as glucose and insulin metabolism in geldings, mares, and stallions. During the course of these experiments, differences in leptin concentrations in the horse were attributed to degree of body condition, gender, and feeding time. …

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The Diagnosis Of Dementia In Persons With Mental Retardation: Validating Methods Of Assessment, Joanne Bielecki Jan 2004

The Diagnosis Of Dementia In Persons With Mental Retardation: Validating Methods Of Assessment, Joanne Bielecki

LSU Doctoral Dissertations

The usual assessment instruments for the diagnosis of dementia are often difficult to use among individuals with mental retardation. This study investigates whether a modified method based on Visser et al. (1997) can identify a dementing process. Ninety individuals diagnosed with severe and profound mental retardation were studied. One half of the participants were diagnosed with Down's syndrome. The participants were equally assigned to one of three groups based on perceived risk of dementia. A differential prevalence design was used. Both cross sectional and longitudinal analyses were utilized in this study. Results indicate that the Visser et al. (1997) method …

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Molecular, Genetic And Physiological Characterization Of A Chlamydomonas Reinhardtii Insertional Mutant, James Edward,Iv Adams Jan 2004

Molecular, Genetic And Physiological Characterization Of A Chlamydomonas Reinhardtii Insertional Mutant, James Edward,Iv Adams

LSU Doctoral Dissertations

Photosynthetic microorganisms must acclimate to environmental conditions that may lead to photo-oxidative stress, such as low CO2 environments or high light intensities. Chlamydomonas reinhardtii, a unicellular, green alga with a Carbon Concentrating Mechanism (CCM), effectively accumulates inorganic carbon (Ci) to levels higher than external concentrations. The CCM concentrates inorganic carbon around Rubisco to increase the CO2 fixation efficiency of C. reinhardtii. Photorespiration, the water-water cycle, the xanthophyll cycle, and the CCM are adaptations that also prevent the over-reduction of photosystems and thus photoinhibition by dissipating the energy from the absorption of excess photons. In an effort …

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Faunal Succession Of Necrophilous Insects Associated With High-Pofile Wildlife Carcasses In Louisiana, Erin Jean Grindley Watson Jan 2004

Faunal Succession Of Necrophilous Insects Associated With High-Pofile Wildlife Carcasses In Louisiana, Erin Jean Grindley Watson

LSU Doctoral Dissertations

The same entomological criteria used in estimating time of death for humans are applicable for deceased wildlife. Necrophilous insects associated with animal carcasses can provide wildlife law enforcement with valuable information necessary for postmortem interval estimation, and ultimately, to incriminate poachers. The purpose of this research was twofold: to establish species composition, faunal succession patterns, and species and life stage interactions of necrophilous insects associated with three wildlife species; and to identify new methods of evaluating succession patterns using statistical measures. Twenty-one large vertebrate carcasses were monitored throughout decomposition in a mixed flatwood forest in East Baton Rouge Parish, LA …

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The Relation Between Knowledge Of Adhd And Treatment Acceptability In A Multi-Disciplinary Pediatric Clinic, Rebecca Owen Currier Jan 2004

The Relation Between Knowledge Of Adhd And Treatment Acceptability In A Multi-Disciplinary Pediatric Clinic, Rebecca Owen Currier

LSU Doctoral Dissertations

Behavior modification and medication have been proven to be the most effective interventions for children with ADHD. For these treatments to be effective, utilization of mental health care services as well as compliance with treatment recommendations is necessary. There has been shown lower care utilization among minorities for the treatment of behavioral disorders. In addition, lack of adherence among these populations to ADHD treatment is not explained by SES, parenting stress, or family coping. An alternative explanation may be parental knowledge of ADHD and opinions of commonly used treatments. The present investigation examined the effect of a brief knowledge intervention …

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Adult Attachment Styles, Children's Self-Competence, And Children's Cognitive Ability: An Ecological Study, Elizabeth Benchea Block Jan 2004

Adult Attachment Styles, Children's Self-Competence, And Children's Cognitive Ability: An Ecological Study, Elizabeth Benchea Block

LSU Doctoral Dissertations

This study investigated children's perceived self-competence and its relationship to the social antecedent of adult attachment and the outcome of children's cognitive ability. Utilizing a predominantly African American sample, 154 mothers, 80 fathers and 205 children were included in this cross-sectional study of second and fourth grade children. Regression analyses indicated that the exploratory relationship between adult attachment as a predictor of children's self-competence was upheld with both second and fourth grade children of participating mothers and fathers. More specifically, maternal attachment was a significant predictor of second grade children's perceived physical competence, social acceptance, and maternal acceptance. Maternal attachment …

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Synthesis, Characterization And Study Of Novel Reagents For The Detection Of Saccharides And Amino Acids, Nadia Nadette St. Luce Jan 2004

Synthesis, Characterization And Study Of Novel Reagents For The Detection Of Saccharides And Amino Acids, Nadia Nadette St. Luce

LSU Doctoral Dissertations

The design of synthetic receptors for the recognition and sensing of saccharides and amino acids is currently a major challenge. This is due to inherent structural similarity and a lack of chromophoric or fluorophoric properties of these compounds. The synthesis and study of novel detection agents for bioactive molecules as well as mechanistic studies are presented herein. The elucidation of the mechanism by which tetraarylboronic acid resorcinarene interact with sugar molecules and promote a solution color change is explored. This vast collaborative study, reveals that DMSO solutions of boronic acid functionalized resorcinarene macrocycles afford visual color changes upon heating or …

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A New Broad Spectrum Disinfectant Suitable For The Food Industry, Giovanna Aita Dequeiroz Jan 2004

A New Broad Spectrum Disinfectant Suitable For The Food Industry, Giovanna Aita Dequeiroz

LSU Doctoral Dissertations

A unique biocide composition (patent pending) that is formed from a hydrogen peroxide and sodium hypochlorite mixture was investigated. A biocidal "complex" is formed by adding the peroxide to the hypochlorite in an amount so that the weight ratio of the peroxide to the hypochlorite is no less than 1:10. The chemical structure of this biocidal "complex" is uncertain but we postulate that it is a semi-stable complex, whose stability is disrupted by heat, acid, U/V exposure and the presence of organic matter (i.e., microbes) The antimicrobial activity of the biocidal "complex" is most likely a combination effect between oxidation …

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Modulation Of Gabaergic Signaling In Retinal Amacrine Cells, Brian Kevin Hoffpauir Jan 2004

Modulation Of Gabaergic Signaling In Retinal Amacrine Cells, Brian Kevin Hoffpauir

LSU Doctoral Dissertations

The intricate circuitry formed by amacrine cells in the inner plexiform layer (IPL) of the retina suggests that these interneurons play a major role in shaping the visual message. The majority of amacrine cells in the vertebrate retina are GABAergic. Thus, elucidating how GABAergic signaling is modulated in the IPL is critical in order to understand how the visual message is processed in the retina. The results presented here suggest that GABAergic signaling between amacrine cells can be modulated by the activation of metabotropic glutamate receptor 5 (mGluR5) or by the production of the second messenger, nitric oxide (NO). A …

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Influence Of The Marsh Edge On The Structure And Trophic Ecology Of The Fish And Macroinvertebrate Community In A Louisiana Estuarine Ecosystem, Guillermo Duque Jan 2004

Influence Of The Marsh Edge On The Structure And Trophic Ecology Of The Fish And Macroinvertebrate Community In A Louisiana Estuarine Ecosystem, Guillermo Duque

LSU Doctoral Dissertations

Habitat degradation of saltmarsh ecosystems reduces the suitability of the marsh as permanent environments for resident species and seasonal nursery grounds for transient fauna. This study was conducted in Terrebonne-Timbalier bays near Cocodrie, Louisiana. Fishes and macroinvertebrates were collected using a drop sampler in marsh-edge and open-water habitat types, in three locations (i.e., pond, channel, and bay), and four seasons (Fall of 2000, Winter of 2000-2001, and Spring and Summer of 2001). The specific objectives of this study were: (1) to determine the difference in composition and structure of fish and macroinvertebrate communities, (2) to establish the food habits of …

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"I Pray It Happens In My Lifetime": The Life History Of Clara Byrd Glasper, A Black Woman Educator Fighting For Educational Equality, Carol Marie Miller Jan 2004

"I Pray It Happens In My Lifetime": The Life History Of Clara Byrd Glasper, A Black Woman Educator Fighting For Educational Equality, Carol Marie Miller

LSU Doctoral Dissertations

The purpose of this study is to write the life history of a black woman educator is order to enhance our understandings of educational activism and social change in the Deep South. African American educators have been marginalized and under represented in their communities despite the roles they have played in ameliorating educational inequalities. The life history of seventy-year-old Clara Byrd Glasper’s activism as a black woman educator is one example. Life history, as a method of research, reveals an individual’s life experiences from their perspective and provides the appropriate methodology to explore the following research questions: In the context …

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Dna Binding Properties Of Histone-Like Protein Hu From Deinoccus Radiodurans Suggest Involvement In Dna Recombination, Sharmistha Ghosh Jan 2004

Dna Binding Properties Of Histone-Like Protein Hu From Deinoccus Radiodurans Suggest Involvement In Dna Recombination, Sharmistha Ghosh

LSU Doctoral Dissertations

The Histone-like protein HU is ubiquitous in eubacteria. Usually with a length of ~90 amino acids, they are predominantly homodimeric, with sequence and structural homology. Escherichia coli HU is involved in DNA repair and recombination. The crystal structure of Anabaena HU shows that it binds DNA with prolines intercalating into the DNA backbone, introducing two kinks at a spacing of 9 bp and bending the DNA through a variable angle of 105-140°. Deinococcus radiodurans is a gram positive mesophile, capable of reconstituting its genome from 1000-2000 double strand breaks incurred due to exposure to environmental extremes. In the first study, …

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The Use Of Altrenogest To Control Reproductive Function In Beef Cattle, Clarence Edward Ferguson Jan 2004

The Use Of Altrenogest To Control Reproductive Function In Beef Cattle, Clarence Edward Ferguson

LSU Doctoral Dissertations

There has been great controversy involving progesterone (P4) levels during early pregnancy in cattle. The objectives of these experiments were to determine the effect of an early low dose administration of P4 or altrenogest (ALT) on pregnancy rates in repeat breeder (RPB) females, if an increase in pregnancy rates could result from a direct effect of P4 on the embryo and if ALT could support pregnancy in the absence of a functional CL. Firstly, ALT was evaluated for use as a progestin in cattle by synchronizing estrus in beef heifers. There were no differences in the …

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Synthesis And Characterization Of Superparamagnetic Silica--Homopolypeptide Composite Particles, Sibel Turksen Jan 2004

Synthesis And Characterization Of Superparamagnetic Silica--Homopolypeptide Composite Particles, Sibel Turksen

LSU Doctoral Dissertations

Hierarchical construction and characterization of core-shell composite particles of interest are presented. The research described shows interest in new types of polymers that provide chirality and responsiveness which have been ignored for so long. The general synthetic design described in this document can be readily used as a model for other systems. Core-shell composite systems of interest consist of silica or silica-coated cobalt cores grafted with a homopolypeptide shell, mostly poly(epsilon-carbobenzyloxy-L-lysine) or poly(gamma-benzyl-L-glutamate). Core particles were decorated with amino groups through a silylation reaction. The amino groups initiated the polymerization (with attachment) of N-carboxyanhydride (NCA) monomers, resulting in a homopolypeptide …

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Transfer Functions Of Streptomyces Plasmid Pij101: Insights Into A Novel Bacterial Conjugation System, Matthew John Ducote Jan 2004

Transfer Functions Of Streptomyces Plasmid Pij101: Insights Into A Novel Bacterial Conjugation System, Matthew John Ducote

LSU Doctoral Dissertations

For Streptomyces plasmid pIJ101 to mediate DNA transfer, only the clt locus and the Tra protein are required, though three non-essential spread functions increase the radial distance of plasmid dissemination among recipients after the initial lateral transfer event. clt is a contiguous 54-bp sequence within an intrinsically curved plasmid region that contains inverted and direct repeats; biochemical and genetic methods described here indicate that single-stranded cleavage reminiscent of most conjugative plasmids does not occur at clt. clt therefore likely promotes plasmid transfer by interacting with the pIJ101 Tra protein or host-encoded factors in a transient, novel DNA-processing event or by …

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The Influence Of Ethnic Embeddedness On The Gender Role Attitudes And Division Of Household Labor Of Married Hispanic Women, Susana Guerra Jan 2004

The Influence Of Ethnic Embeddedness On The Gender Role Attitudes And Division Of Household Labor Of Married Hispanic Women, Susana Guerra

LSU Doctoral Dissertations

In this study I examine the effects of both network structure and network function on the gender role attitudes and division of household labor among Hispanic women. Using a representative sub-sample drawn from the first wave of the National Study of Families and Households, I determine to what extent network processes help explain the gender role attitudes and behaviors of Hispanic women. Specifically, I focus on how embeddedness within a Hispanic community, as well as a woman’s level of social support exchange with kin and non-kin help explain her current gender role attitudes and household labor allocation. I found that …

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Content Analysis Of The Leading General Academic Databases, Kelly D. Blessinger, Maureen Olle-Lajoie Jan 2004

Content Analysis Of The Leading General Academic Databases, Kelly D. Blessinger, Maureen Olle-Lajoie

Faculty Publications

Title lists from the three leading general academic databases were compared to evaluate journal content. Some of the elements that were analyzed include subject coverage, the quality of titles provided, accessibility issues, and trends over time for these databases. While database title lists are now readily available on the Internet for comparison, as our article illustrates, these lists need further evaluation for proper assessment.

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The Syntheses Of Novel Indicators And Materials For Chiral Separation, Rolanda Johnson Jan 2004

The Syntheses Of Novel Indicators And Materials For Chiral Separation, Rolanda Johnson

LSU Doctoral Dissertations

The colorimetric properties of resorcinarene solutions had not been fully investigated since von Baeyer's initial synthesis. We find that the solutions containing resorcinarene macrocycles develop color upon heating or standing. In the presence of saccharides, these solutions exhibit significant color changes which are readily observable by visual inspection. We determined that the solution color is due to macrocycle ring opening and oxidation. The optical responses to saccharides are due to complexation of the sugar with the acyclic chromophores. We have applied these mechanistic insights towards the challenging problems of the visual detection of saccharides and other important biologically analytes. In …

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An Evaluation Of Reference Evapotranspiration Models In Louisiana, Royce Landon Fontenot Jan 2004

An Evaluation Of Reference Evapotranspiration Models In Louisiana, Royce Landon Fontenot

LSU Master's Theses

Daily and monthly output from seven evapotranspiration models (FAO-24 Radiation, FAO-24 Blaney-Criddle, Hargreaves-Samani, Priestly-Taylor, Makkink, and Turc) have been tested against reference evapotranspiration data computed by the FAO-56 Penman-Monteith model to assess the accuracy of each model in estimating grass-reference evapotranspiration in Louisiana. Models were compared at eight stations of the Louisiana Agriclimate Information System using data from December 2002 to November 2003. Comparisons were also made using three composite regions: statewide, inland, and coastal. A pan evaporation to reference evapotranspiration model (FAO-24 Pan Evaporation) was also tested against daily grass reference evapotranspiration from the FAO-56 Penman-Monteith model using data …

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Estimation Of Living Body Weight Based On Measurements Of Anterior Superior Iliac Spine Breadth And Stature, Jaime A. Suskewicz Jan 2004

Estimation Of Living Body Weight Based On Measurements Of Anterior Superior Iliac Spine Breadth And Stature, Jaime A. Suskewicz

LSU Master's Theses

Standard identification criteria for creating a decedent’s biological profile typically include ancestry, sex, age, and stature, but not body weight. Body weight information may not only assist in creating a more complete biological profile but may also provide insight into other forensic considerations, such as taphonomy and body transport and disposal. The current study seeks to establish multiple regression equations for the prediction of living body weight in skeletal remains. Specifically, the measurements of anterior superior iliac spine (ASIS) breadth and stature are assessed with regard to living weight. Research was carried out on both a skeletal sample and a …

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Tissue Interactions With Lasers And Liquid Nitrogen: A Novel Cryopreservation Method, Deepak Kandra Jan 2004

Tissue Interactions With Lasers And Liquid Nitrogen: A Novel Cryopreservation Method, Deepak Kandra

LSU Master's Theses

The importance of the imposed cooling rate in cryopreserving native cells and tissues has been long recognized in the field of cryobiology. When biological tissues are subjected to cooling rates in excess of thousands of degree C per minute, the characteristic structural and physical manifestations of the ice formed are such that the traditional damage due to ice formation at lower cooling rates are suppressed. Hence, achieving high cooling rates in tissues and cells of biologically relevant sizes (mm’s and cm’s) has been a long standing research problem. In the present study, we present a novel technique to achieve high …

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Chromosomal Localization Of A Proinsulin Transgene Inserted With A Transposon-Based Vector Into Japanese Quail, Coturnix Coturnix, Lacey R. Mcnally Jan 2004

Chromosomal Localization Of A Proinsulin Transgene Inserted With A Transposon-Based Vector Into Japanese Quail, Coturnix Coturnix, Lacey R. Mcnally

LSU Doctoral Dissertations

The overall goals of this research were to develop a reproducible method of detecting stable DNA insertion into Japanese quail and provide a method for gene location on avian chromosomes. This research resulted in the development of a different method of obtaining chromosome spreads in Japanese quail, the establishment of primed in situ hybridization as a method for the chromosomal gene detection in birds, development of Teflon-coated coverslip slides to facilitate laser microdissection of 0.5 ƒÝm samples, and chromosomal identification of proinsulin transgene insertions by laser microdissection and nucleotide sequence from G2 Japanese quail. The 28S rDNA was found on …

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