Digital Commons Network^™

Optimization Of The Self-Healing Efficiency Of Bacterial Concrete Using Impregnation Of Three Different Precursors Into Lightweight Aggregate, Omar Omar

LSU Master's Theses

Concrete is the most broadly used construction material; thus, developing sustainable concrete is essential to decrease greenhouse gas (GHG) emissions from concrete production. Implementation of self-healing concrete technologies is a promising approach to enhance the durability and sustainability of the transportation infrastructure. Among these technologies, bacterial concrete has the potential to seal microcracks through microbial induced calcite precipitation (MICP). Bacterial protection is essential to ensure the viability of this technology due to concrete’s harsh environment. Additionally, the success of this technology depends on the presence of an adequate mineral precursor compound and nutrient for the bacteria. As such, the main …

Navigating Political Discussions Online: The Role Safe Spaces Play In Women's Political Participation On Twitter, Ayla Oden

LSU Doctoral Dissertations

This study analyzes how women navigate political discussions online. Social media breeds high-conflict spaces where threats and incivility are more likely to persist. To avoid ad hominem attacks or harassment from online, male users, women may be more likely to use “safe spaces” online, including stereotypically feminine conversations regarding “women’s issues.” The purpose of this study is to analyze how women discuss politics online and whether there are differences in the issues discussed by men and women on Twitter. Using three studies, I examined these differences and their relationship to the threats women face online and their political behavior offline. …

Rats Lacking Ucp1 Present A Novel Translational Tool For The Investigation Of Thermogenic Adaptation During Cold Challenge, Jaycob D. Warfel, Carrie M. Elks, David S. Bayless, Bolormaa Vandanmagsar, Allison C. Stone, Samuel E. Velasquez, Paola Olivares-Nazar, Robert C. Noland, Sujoy Ghosh, Jingying Zhang, Randall L. Mynatt

Division of Basic Science Faculty Publications

Aim: Valuable studies have tested the role of UCP1 on body temperature maintenance in mice, and we sought to knockout Ucp1 in rats (Ucp1−/−) to provide insight into thermogenic mechanisms in larger mammals. Methods: We used CRISPR/Cas9 technology to create Ucp1−/− rats. Body weight and adiposity were measured, and rats were subjected to indirect calorimetry. Rats were maintained at room temperature or exposed to 4°C for either 24 h or 14 days. Analyses of brown and white adipose tissue and skeletal muscle were conducted via histology, western blot comparison of oxidative phosphorylation proteins, and qPCR to compare mitochondrial DNA levels …

Bayesian Order Constrained Adaptive Design For Phase Ii Clinical Trials Evaluating Subgroup-Specific Treatment Effect, Mu Shan, Beibei Guo, Hao Liu, Qian Li, Yong Zang

Faculty Publications

The “one-size-fits-all’’ paradigm is inappropriate for phase II clinical trials evaluating biotherapies, which are often expected to have substantial heterogeneous treatment effects among different subgroups defined by biomarker. For these biotherapies, the objective of phase II clinical trials is often to evaluate subgroup-specific treatment effects. In this article, we propose a simple yet efficient Bayesian adaptive phase II biomarker-guided design, referred to as the Bayesian-order constrained adaptive design, to detect the subgroup-specific treatment effects of biotherapies. The Bayesian order constrained adaptive design combines the features of the enrichment design and sequential design. It starts with a “all-comers” stage, and subsequently …

Mathematics Library News, Aaron Lercher

Faculty Publications

Mathematics Library News is an LSU Libraries newsletter for members of the Mathematics and Experimental Statistics Departments, published roughly once each semester.

Niche Specialization And Community Niche Space Increase With Species Richness In Filter-Feeder Assemblages, Irene Sánchez González, Garrett W. Hopper, Jamie R. Bucholz, Megan E. Kubala, Jeffrey D. Lozier, Carla L. Atkinson

Faculty Publications

Ecological theory posits that higher species richness should be associated with greater exploitation of resources and niche packing resulting from either increasing species niche overlap or specialization of species' niches. Research evaluating niche theory in animals tends to focus on organisms among functional feeding guilds, while resource partitioning might be more critical within functional groups. Freshwater mussels (Family: Unionidae) are a diverse and imperiled group of animals that are ideal models to test niche occupancy due to their functional similarity as filter-feeders and their occurrence in spatially and temporally stable multispecies aggregations. We evaluated the relationship between species richness and …

Tnfα Effects On Adipocytes Are Influenced By The Presence Of Lysine Methyltransferases, G9a (Ehmt2) And Glp (Ehmt1), Ashley A. Able, Allison J. Richard, Jacqueline M. Stephens

Division of Basic Science Faculty Publications

Impaired adipocyte function contributes to systemic metabolic dysregulation, and altered fat mass or function increases the risk of Type 2 diabetes. EHMTs 1 and 2 (euchromatic histone lysine methyltransferases 1 and 2), also known as the G9a-like protein (GLP) and G9a, respectively, catalyze the mono- and di-methylation of histone 3 lysine 9 (H3K9) and also methylate nonhistone substrates; in addition, they can act as transcriptional coactivators independent of their methyltransferase activity. These enzymes are known to contribute to adipocyte development and function, and in vivo data indicate a role for G9a and GLP in metabolic disease states; however, the mechanisms …

Latino Paradox Or Black Exception? Race, Ethnicity, And Crime In The 21st Century, Edward S. Shihadeh, Raymond E. Barranco

Faculty Publications

George Floyd’s murder by a Minnesota police officer sparked outrage, protests, and a re-evaluation of racial inequities in America. Within criminology, we argue, that re-evaluation should include the Latino Paradox, the idea that Latino communities are an exception—a paradox—in that, while they face economic deprivation, they also possess a magical something that makes them resistant to social problems like crime. Unfortunately, this compels the more delicate question; what is the deficiency in Black communities that makes them so vulnerable to crime? However, as we argue here, the Latino Paradox forces a false comparison. Its assumptions with respect to crime are …

Amazonian Birds In More Dynamic Habitats Have Less Population Genetic Structure And Higher Gene Flow, Oscar Johnson, Camila C. Ribas, Alexandre Aleixo, Luciano N. Naka, Michael G. Harvey, Robb T. Brumfield

Faculty Publications

Understanding the factors that govern variation in genetic structure across species is key to the study of speciation and population genetics. Genetic structure has been linked to several aspects of life history, such as foraging strategy, habitat association, migration distance, and dispersal ability, all of which might influence dispersal and gene flow. Comparative studies of population genetic data from species with differing life histories provide opportunities to tease apart the role of dispersal in shaping gene flow and population genetic structure. Here, we examine population genetic data from sets of bird species specialized on a series of Amazonian habitat types …

Effect Of Exercise Training On Insulin-Stimulated Glucose Disposal: A Systematic Review And Meta-Analysis Of Randomized Controlled Trials, Candida J. Rebello, Dachuan Zhang, John P. Kirwan, Adam C. Lowe, Carlante J. Emerson, Chelsea L. Kracht, Lori C. Steib, Frank L. Greenway, William D. Johnson, Justin C. Brown

Division of Basic Science Faculty Publications

Background and objective: The effect of exercise training on whole-body insulin sensitivity has not been systematically summarized. We aimed to summarize the data from randomized controlled trials evaluating the effect of exercise training on insulin action, in adults. Subjects: MEDLINE, EMBASE, and CENTRAL databases were searched until January 2021. Randomized controlled trials lasting ≥4 weeks, including adults, and evaluating the effect of exercise on insulin-stimulated glucose disposal measured using the hyperinsulinemic euglycemic clamp, were included. Methods: Three reviewers extracted summary data from published trials. The primary outcome was insulin-stimulated glucose disposal. Standardized weighted mean differences (SMD) in glucose disposal between …

Handling And Restraint Induce A Significant Increase In Plasma Corticosterone In Hispaniolan Amazon Parrots (Amazona Ventralis), Shelby N. Parks, Thomas N. Tully, Aspen L. Settle, Christine R. Lattin

Faculty Publications

OBJECTIVE: To measure baseline plasma corticosterone levels in Hispaniolan Amazon parrots (Amazona ventralis) and assess the effects of handling and restraint on corticosterone levels over 1 hour, reflective of what parrots might experience during veterinary care. ANIMALS: 10 male and 12 female Hispaniolan Amazon parrots. PROCEDURES: Each parrot was removed from its cage and wrapped in a towel for restraint similar to that performed in a clinical setting. An initial baseline blood sample was collected in < 3 minutes upon entrance into the parrot room, after which blood samples were taken every 15 minutes for 1 hour (a total of 5 blood samples). An enzyme-linked immunoassay was validated for Hispaniolan Amazon parrots and used to determine concentrations of plasma corticosterone. RESULTS: On average, parrots showed a significant increase in corticosterone between baseline samples and all subsequent postrestraint time points (average baseline corticosterone ± SD: 0.51 ± 0.65 ng/mL). Females, on average, displayed significantly higher corticosterone levels than males after 30, 45, and 60 minutes of restraint (P = .016, P = .0099, and P = .015, respectively). Birds with feather-destructive behavior did not have significantly higher corticosterone levels than birds without the condition (P = .38). CLINICAL RELEVANCE: Understanding the physiological stress response in companion psittacine birds during routine handling will allow clinicians to better evaluate how this may affect the patient's condition and diagnostic test results. Assessing how corticosterone correlates to behavioral conditions such as feather-destructive behavior will provide clinicians with the potential to develop treatment options.

Improvement Of Existing Stage-Top Incubator For Cell Imaging (Stici), Alyson Goree

Honors Capstones

No abstract provided.

Characterization Of Leptin Receptor Expressing Cells In The Cortex And Hippocampus Of The Mouse Brain, Cade Canepa

Honors Capstones

No abstract provided.

The Intersectionality Of Race And Gender And Its Relationship To School Belonging And Student Mental Health, Kennedi Boatner

Honors Capstones

No abstract provided.

The Evolution Of Pop Poetry: The Legacy Of Mina Loy, Sylvia Plath, And Anne Sexton's Contributions To Female-Centered Art And Expression, Madeline Ballentine

Honors Capstones

No abstract provided.

Investigating The Role Of The Drosophila Melanogaster Beaf Protein In Mediating Long Distance Chromatin Looping, Oluwatobi Ojemakinde

Honors Capstones

No abstract provided.

Assessing The Application Of Heat Map Technology In Microscopic Analysis Of Saw Marks On Bone, Ian Plunkett

Honors Capstones

No abstract provided.

Differential Expression Analysis Of Dorsal And Ventral Retinas Of The Four-Eyed Fish Anableps Anableps, Andrea Matus

Honors Capstones

No abstract provided.

Regenerative Medicine For Tendon/Ligament Injuries: De Novo Equine Tendon/Ligament Neotissue Generation And Application, Takashi Taguchi

LSU Doctoral Dissertations

Tendon and ligament injuries are debilitating conditions across species. Poor regenerative capacities of these tissues limit restoration of original functions. The first study evaluated the effect of cellular administration on tendon/ligament injuries in horses using meta-analysis. The cellular administration was effective in restoring ultrasonographic echogenicity and increasing vascularity during early phase of healing. Additionally, it improved microstructural organization of healed tissue in terms of cellularity and fiber alignment. However, the study did not support its use for increasing rate of return to performance, expression/deposition of tendon-specific genes/proteins, or mechanical properties.

The findings led to the second study that engineered implantable …

Decolonial Queer Poetic, Aparajita Dutta

LSU Doctoral Dissertations

Queer bodies, produced by poetry, conjure dissident bodies who exist as citizens within the scope of the nation. In this analysis, I take into consideration Indian queer poetry, from both India and the diaspora, because bodies produced through this poetry reveal intersectional identities of queerness like race, and caste, that will help us understand how non-normative bodies exist along with other forms of marginalized identities like caste and race. In absence of academic scholarship on Indian queer poetry’s connection to the construction of nationhood, I aim to survey Indian queer poetry from mainland India and the Northern American diaspora, and …

Integrating Germplasm Repositories Into Oyster Aquaculture Systems At The Pathway, Center, And Network Levels, Sarah R. Bodenstein

LSU Doctoral Dissertations

Germplasm repositories offer many potential benefits to commercial farm operations, including preserving genetic resources and increasing production capabilities. Aquaculture industries have yet to take advantage of repository storage despite decades of research evaluating cryopreservation protocols. Eastern oysters (Crassostrea virginica), for example, are a useful research species when addressing this problem. Oysters are an economically important aquaculture species and a high-throughput cryopreservation method for oysters already exists. To address the gap between protocol research and commercial application a different approach is necessary that can analyze the requirements of repository development at multiple organizational levels. The goal of this dissertation was to …

Universal Screening For Student Mental Health: Selection Of Norming Sample As A Determinate Of Risk Status, Meagan Zeitvogel

LSU Master's Theses

The prevalence of student mental health concerns has increased the need for universal mental health screening. In turn, students may have increased access to services and support. Some screeners determine risk status by comparing student scores to norming samples that can be based solely on age (i.e., combined-gender) or on age and gender (i.e., separate-gender). Combined-gender norming samples assign risk status based on typical emotional and behavioral functioning across development. Separate-gender norming samples assign risk status based on developmental and biological differences between boys and girls. Currently, there is no research determining if students’ risk status changes based on norming …

Functional Domains Of The Hsv-1 Ul37 Protein In Virus Infectivity And Retrograde Transport In Neurons, Carolyn Clark

LSU Doctoral Dissertations

Herpes simplex virus type 1 is large alphaherpesvirus with 20 envelope proteins and 23 tegument proteins. Virus entry is via fusion of the viral envelope with cellular membranes mediated by a complex of viral glycoproteins. Glycoprotein B (gB) is the only fusogenic glycoprotein, while other viral glycoproteins and membrane proteins including gD, gH, gL, gK and UL20 membrane protein modulate gB’s fusogenicity. Previously, Our laboratory developed the HSV-1 (VC2) vaccines strain with partial deletions in the amino termini of gK (ΔgK(31–68)) and the UL20 membrane protein (ΔUL20(4–22)). VC2 enters epithelial cells only via endocytosis followed by release of the capsid …

Kinetic And Chemical Effects Of Clays And Other Fillers In The Preparation Of Epoxy-Vinyl Ether Composites Using Radical-Induced Cationic Frontal Polymerization, Brecklyn R. Groce, Emma E. Lane, Daniel P. Gary, Douglas T. Ngo, Dylan T. Ngo, Fahima Shaon, Jorge A. Belgodere, John A. Pojman

Faculty Publications

Addition of fillers to formulations can generate composites with improved mechanical properties and lower the overall cost through a reduction of chemicals needed. In this study, fillers were added to resin systems consisting of epoxies and vinyl ethers that frontally polymerized through a radical-induced cationic frontal polymerization (RICFP) mechanism. Different clays, along with inert fumed silica, were added to increase the viscosity and reduce the convection, results of which did not follow many trends present in free-radical frontal polymerization. The clays were found to reduce the front velocity of RICFP systems overall compared to systems with only fumed silica. It …

Injectable Antioxidant And Oxygen-Releasing Lignin Composites To Promote Wound Healing, Swathi Balaji, Walker D. Short, Benjamin W. Padon, Jorge A. Belgodere, Sarah E. Jimenez, Naresh T. Deoli, Anna C. Guidry, Justin C. Green, Tanuj J. Prajapati, Fayiz Farouk, Aditya Kaul, Dongwan Son, Olivia S. Jung, Carlos E. Astete, Myungwoong Kim, Jangwook P. Jung

Faculty Publications

The application of engineered biomaterials for wound healing has been pursued since the beginning of tissue engineering. Here, we attempt to apply functionalized lignin to confer antioxidation to the extracellular microenvironments of wounds and to deliver oxygen from the dissociation of calcium peroxide for enhanced vascularization and healing responses without eliciting inflammatory responses. Elemental analysis showed 17 times higher quantity of calcium in the oxygen-releasing nanoparticles. Lignin composites including the oxygen-generating nanoparticles released around 700 ppm oxygen per day at least for 7 days. By modulating the concentration of the methacrylated gelatin, we were able to maintain the injectability of …

Development, Survival, And Fecundity Of Helicoverpa Zea (Lepidoptera: Noctuidae) On Five Weedy Host Plants Common Across The Mid-South Usa, James P. Glover, Maribel Portilla, Gadi V.P. Reddy

Faculty Publications

Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) is a highly mobile and polyphagous pest known to feed on over 300 cultivated and wild host plant species. Larvae cause significant economic damage to several commercial row crops over multiple generations each growing season. The objective of this study was to compare biological fitness of H. zea on 5 weedy host plants commonly found across the mid-south US. Crimson clover (Trifolium incarnatum L.), white clover (Trifolium repens L.), hairy vetch (Vicia villosa Roth), kudzu (Pueraria montana [Lour.] Merr. (all Fabaceae), and honeysuckle (Lonicera japonica Thunb.; Caprifoliaceae) were evaluated as primary food sources for H. …

The Reality Of Teaching English Virtually: Esl Teachers' Perspectives And Experiences During The Covid-19 National Pandemic, Natalia Guerrero

LSU Doctoral Dissertations

This study examined the dilemma ESL teachers experienced as the educational system shifted from the usual modus operandi of in-person lessons to the uncharted virtual learning environment (VLE). ESL teachers, in one of the largest urban districts in Louisiana, accumulated additional roles and responsibilities that were unique to the teachers of the English learner (EL) population enrolled at their schools.

Data collected to answer the research questions were the product of single and focus group’s interviews with five ESL elementary and middle school teachers in Freedom District. State and district emergency response to COVID-19 guidelines, along with instructional artifacts, were …

The Characterization Of Selected Α- And Β-Carbonic Anhydrases Of Arabidopsis Thaliana, Hiruni Weerasooriya

LSU Doctoral Dissertations

Carbonic anhydrases (CAs) are zinc-metalloenzymes that interconvert two inorganic carbon (Ci) species, CO₂ and HCO₃^-. In Arabidopsis thaliana, there are eight αCA genes, six βCA genes, three γCA genes, and two γCA-like genes. The majority of CA research in plants has focused on finding a link between CA activity and photosynthesis rates. Since the CA genes are expressed in different plant tissues and multiple CA isoforms are distributed among various organelles of the plant cell, I hypothesize that CAs facilitate CO₂ diffusion among cell compartments and maintain Ci pools for carbon-requiring reactions by interconverting …

Optimization Of Host Cell-Compatible, Antimicrobial Peptides Effective Against Biofilms And Clinical Isolates Of Drug-Resistant Bacteria, Jenisha Ghimire, Robert J. Hart, Anabel Soldano, Charles H. Chen, Shantanu Guha, Joseph P. Hoffmann, Kalen M. Hall, Leisheng Sun, Benjamin J. Nelson, Timothy K. Lu, Jay K. Kolls, Mario Rivera, Lisa A. Morici, William C. Wimley

Faculty Publications

Here, we describe the continued synthetic molecular evolution of a lineage of host-compatible antimicrobial peptides (AMP) intended for the treatment of wounds infected with drug-resistant, biofilm-forming bacteria. The peptides tested are variants of an evolved AMP called d-amino acid CONsensus with Glycine Absent (d-CONGA), which has excellent antimicrobial activities in vitro and in vivo. In this newest generation of rational d-CONGA variants, we tested multiple sequence-structure-function hypotheses that had not been tested in previous generations. Many of the peptide variants have lower antibacterial activity against Gram-positive or Gram-negative pathogens, especially variants that have altered hydrophobicity, secondary structure potential, or spatial …

Assessing The Nutritional Value Of Dubia Roaches (Blaptica Dubia) As A Feeder Insect In Reptiles, Elise Barras

LSU Master's Theses

Dubia roaches (Blaptica dubia) have emerged on the market as a high protein and low-fat alternative to other feeder insects for captive reptiles. However, much like other insects, dubia roaches naturally have an inverse calcium to phosphorus (Ca:P) ratio. Offering insects to reptiles that are deficient in calcium can lead to nutritional disease (e.g., nutritional secondary hyperparathyroidism). For dubia roaches to be a valuable feeder insect, their Ca:P ratio must be corrected. Several studies have assessed their nutritional composition, but no studies have manipulated the calcium content of these insects. Using a high calcium (8.3% calcium, dry matter) …