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Highly Water-Soluble Thermally Responsive Poly(Thiophene)-Based Brushes, Sreelatha S. Balamurugan, Grigor B. Bantchev, Yuming Yang, Robin L. Mccarley Aug 2005

Highly Water-Soluble Thermally Responsive Poly(Thiophene)-Based Brushes, Sreelatha S. Balamurugan, Grigor B. Bantchev, Yuming Yang, Robin L. Mccarley

Faculty Publications

(Figure Presented) A brush with success: Responsive brushes composed of poly(N-isopropylacrylamide) grafted onto a poly(thiophene) backbone display the highest water solubility ever reported for a neutral poly(thiophene) as well as temperature-dependent optical properties and water solubilities (see picture). The graft polymer exhibits shifts in the π-π* transition of the poly(thiophene) backbone near the collapse temperature of the poly(N-isopropylacrylamide) chains. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

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A Mechanism For Assembly Of Complexes Of Vitronectin And Plasminogen Activator Inhibitor-1 From Sedimentation Velocity Analysis, Kenneth H. Minor, Christine R. Schar, Grant E. Blouse, Joseph D. Shore, Daniel A. Lawrence, Peter Schuck, Cynthia B. Peterson Aug 2005

A Mechanism For Assembly Of Complexes Of Vitronectin And Plasminogen Activator Inhibitor-1 From Sedimentation Velocity Analysis, Kenneth H. Minor, Christine R. Schar, Grant E. Blouse, Joseph D. Shore, Daniel A. Lawrence, Peter Schuck, Cynthia B. Peterson

Faculty Publications

Plasminogen activator inhibitor-1 (PAI-1) and vitronectin are cofactors involved in pathological conditions such as injury, inflammation, and cancer, during which local levels of PAI-1 are increased and the active serpin forms complexes with vitronectin. These complexes become deposited into surrounding tissue matrices, where they regulate cell adhesion and pericellular proteolysis. The mechanism for their co-localization has not been elucidated. We hypothesize that PAI-1-vitronectin complexes form in a stepwise and concentration-dependent fashion via 1:1 and 2:1 intermediates, with the 2:1 complex serving a key role in assembly of higher order complexes. To test this hypothesis, sedimentation velocity experiments in the analytical …

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Mobilization Of The Iron Centre In Isca For The Iron-Sulphur Cluster Assembly In Iscu, Baojin Ding, Edward S. Smith, Huangen Ding Aug 2005

Mobilization Of The Iron Centre In Isca For The Iron-Sulphur Cluster Assembly In Iscu, Baojin Ding, Edward S. Smith, Huangen Ding

Faculty Publications

The biogenesis of iron-sulphur clusters requires the co-ordinated delivery of both iron and sulphur. It is now clear that sulphur in iron-sulphur clusters is derived from L-cysteine by cysteine desulphurases. However, the iron donor for the iron-sulphur cluster assembly still remains elusive. Our previous studies indicated that Escherichia coli IscA, a member of the iron-sulphur cluster assembly machinery, is an iron-binding protein that can provide iron for the iron-sulphur cluster assembly in a proposed scaffold IscU. To determine how the iron centre in IscA is transferred for the iron-sulphur cluster assembly in IscU, we explore the mobility of the iron …

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Altering The Tropism Of Lentiviral Vectors Through Pseudotyping, James Cronin, Xian Yang Zhang, Jakob Reiser Aug 2005

Altering The Tropism Of Lentiviral Vectors Through Pseudotyping, James Cronin, Xian Yang Zhang, Jakob Reiser

Faculty Publications

The host range of retroviral vectors including lentiviral vectors can be expanded or altered by a process known as pseudotyping. Pseudotyped lentiviral vectors consist of vector particles bearing glycoproteins (GPs) derived from other enveloped viruses. Such particles possess the tropism of the virus from which the GP was derived. For example, to exploit the natural neural tropism of rabies virus, vectors designed to target the central nervous system have been pseudotyped using rabies virus-derived GPs. Among the first and still most widely used GPs for pseudotyping lentiviral vectors is the vesicular stomatitis virus GP (VSV-G), due to the very broad …

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From The Margins Of The Genome: Mobile Elements Shape Primate Evolution, Dale J. Hedges, Mark A. Batzer Aug 2005

From The Margins Of The Genome: Mobile Elements Shape Primate Evolution, Dale J. Hedges, Mark A. Batzer

Faculty Publications

As is the case with mammals in general, primate genomes are inundated with repetitive sequence. Although much of this repetitive content consists of "molecular fossils" inherited from early mammalian ancestors, a significant portion of this material comprises active mobile element lineages. Despite indications that these elements played a major role in shaping the architecture of the genome, there remain many unanswered questions surrounding the nature of the host-element relationship. Here we review advances in our understanding of the host-mobile element dynamic and its overall impact on primate evolution. © 2005 Wiley Periodicals, Inc.

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Phylogeny And Comparative Biogeography Of Pionopsitta Parrots And Pteroglossus Toucans, Jessica R. Eberhard, Eldredge Bermingham Aug 2005

Phylogeny And Comparative Biogeography Of Pionopsitta Parrots And Pteroglossus Toucans, Jessica R. Eberhard, Eldredge Bermingham

Faculty Publications

Studies of Neotropical birds, and their distributions and areas of endemism, in particular, have been central in the formulation of hypotheses proposed to explain the high species diversity in the Neotropics. We used mtDNA sequence data (ATPase 6 and 8, COI, and cyt b) to reconstruct the species-level phylogenies for two genera, Pionopsitta (Aves: Psittacidae) and Pteroglossus (Aves: Ramphastidae), compare our results with previous morphology-based phylogenetic analyses, and estimate the absolute timing of lineage and biogeographic divergences. Both the Pionopsitta and Pteroglossus phylogenies support a hypothesis of area relationships in which a divergence of the Serra do Mar (Atlantic Forest, …

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Species Limits Within The Mexican Garter Snakes Of The Thamnophis Godmani Complex, Douglas A. Rossman, Frank T. Burbrink Jul 2005

Species Limits Within The Mexican Garter Snakes Of The Thamnophis Godmani Complex, Douglas A. Rossman, Frank T. Burbrink

Occasional Papers of the Museum of Natural Science, Louisiana State University

The highly variable Thamniphis godmani complex of southern Mexico, comprised of four apparently allopatric populations, was examined by using 28 morphological characters scored on 214 specimens. Character values were tested with univariate and multivariate statistics to determine if populations are morphologically distinct. The results suggest that the four populations represent independently evolving lineages. Four species, three previously undescribed, are recognized in the T. godmani complex. A lectotype is designated for T. godmani. For each species, a diagnosis description of holotype or lectotype, summary of interspecific variation, and statement of distribution are provided. Comparisons are made with T. errans, alleged to …

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Loss Of Outer Membrane Proteins Without Inhibition Of Lipid Export In An Escherichia Coli Yaet Mutant, William T. Doerrler, Christian R.H. Raetz Jul 2005

Loss Of Outer Membrane Proteins Without Inhibition Of Lipid Export In An Escherichia Coli Yaet Mutant, William T. Doerrler, Christian R.H. Raetz

Faculty Publications

Escherichia coli yaeT encodes an essential, conserved outer membrane (OM) protein that is an ortholog of Neisseria meningitidis Omp85. Conflicting data with TV. meningitidis indicate that Omp85 functions either in assembly of OM proteins or in export of OM lipids. The role of YaeT in E. coli was investigated with a new temperature-sensitive mutant harboring nine amino acid substitutions. The mutant stops growing after 60 min at 44 °C. After 30 min at 44 °C, incorporation of [35S]methionine into newly synthesized OM proteins is selectively inhibited. Synthesis and export of OM phospholipids and lipopolysaccharide are not impaired. OM protein levels …

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Negative Cooperativity Of Uric Acid Binding To The Transcriptional Regulator Hucr From Deinococcus Radiodurans, Steven P. Wilkinson, Anne Grove Jul 2005

Negative Cooperativity Of Uric Acid Binding To The Transcriptional Regulator Hucr From Deinococcus Radiodurans, Steven P. Wilkinson, Anne Grove

Faculty Publications

Members of the MarR family of winged helix transcriptional regulators have been shown to regulate multidrug and oxidative stress response, pathogenesis, and catabolism of aromatic compounds. Many respond to anionic lipophilic compounds in their capacity to bind DNA, and the co-crystal structure of MarR bound to salicylate revealed two ligand-binding pockets, SAL-A and SAL-B. The MarR homolog, HucR, from Deinococcus radiodurans has been shown to repress expression of a predicted uricase, and DNA-binding by HucR is antagonized by uric acid, the substrate of uricase. We provide a biochemical investigation of DNA-binding and uric acid-binding by HucR. Equilibrium analytical ultracentrifugation indicates …

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Enhancement Of Calcium-Dependent Afterpotentials In Oxytocin Neurons Of The Rat Supraoptic Nucleus During Lactation, Ryoichi Teruyama, William E. Armstrong Jul 2005

Enhancement Of Calcium-Dependent Afterpotentials In Oxytocin Neurons Of The Rat Supraoptic Nucleus During Lactation, Ryoichi Teruyama, William E. Armstrong

Faculty Publications

The firing pattern of oxytocin (OT) hormone synthesizing neurons changes dramatically immediately before each milk ejection, when a brief burst of action potentials is discharged. OT neurons possess intrinsic currents that would modulate this burst. Our previous studies showed the amplitude of the Ca2+-dependent afterhyperpolarization (AHP) following spike trains is significantly larger during lactation. In the present study we sought to determine which component of the AHP is enhanced, and whether the enhancement could be related to changes in whole-cell Ca2+ current or the Ca2+ transient in identified OT or vasopressin (VP) neurons during lactation. We confirmed, with whole-cell current-clamp …

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V-Atpase Expression During Development Of Artemia Franciscana Embryos: Potential Role For Proton Gradients In Anoxia Signaling, Joseph A. Covi, Steven C. Hand Jul 2005

V-Atpase Expression During Development Of Artemia Franciscana Embryos: Potential Role For Proton Gradients In Anoxia Signaling, Joseph A. Covi, Steven C. Hand

Faculty Publications

Under anoxia, Artemia franciscana embryos downregulate metabolic processes and approach an ametabolic state. Entrance into this quiescent state is accompanied by a profound acidification of the intracellular space, and more than two decades of research now clearly demonstrates that this acidification is critical to metabolic downregulation in anoxic embryos. However, the proximal mechanisms responsible for the pH shift remain largely unidentified. Here, we report evidence demonstrating expression of the V-ATPase in encysted embryos and present an argument for its involvement in the intracellular acidification induced by anoxia. We identified a single B-subunit cDNA sharing the greatest degree of sequence similarity …

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V-Atpase Inhibition Prevents Recovery From Anoxia In Artemia Franciscana Embryos: Quiescence Signaling Through Dissipation Of Proton Gradients, Joseph A. Covi, W. Dale Treleaven, Steven C. Hand Jul 2005

V-Atpase Inhibition Prevents Recovery From Anoxia In Artemia Franciscana Embryos: Quiescence Signaling Through Dissipation Of Proton Gradients, Joseph A. Covi, W. Dale Treleaven, Steven C. Hand

Faculty Publications

The metabolic downregulation critical for long-term survival of Artemia franciscana embryos under anoxia is mediated, in part, by a progressive intracellular acidification. However, very little is known about the mechanisms responsible for the pH transitions associated with exposure to, and recovery from, oxygen deprivation. In the present study, we demonstrate with 31P-NMR that incubation of intact embryos with the V-ATPase inhibitor bafilomycin A1 severely limits intracellular alkalinization during recovery from anoxia without affecting the restoration of cellular nucleotide triphosphate levels. Based on these data, it appears that oxidative phosphorylation and ATP resynthesis can only account for the first 0.3 pH …

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The Regulation Of Fatty Acid Synthase By Stat5a, Jessica C. Hogan, Jacqueline M. Stephens Jul 2005

The Regulation Of Fatty Acid Synthase By Stat5a, Jessica C. Hogan, Jacqueline M. Stephens

Faculty Publications

Growth hormone (GH) diminishes adipose tissue mass in vivo and decreases expression and activity of fatty acid synthase (FAS) in adipocytes. GH and prolactin (PRL) are potent activators of STAT5 and exert adipogenic and antiadipogenic effects in adipocytes. In this study, we demonstrate that GH and PRL decrease the mRNA and protein levels of FAS in 3T3-L1 adipocytes. We present evidence that indicates that FAS is repressed at the level of transcription. In addition, PRL responsiveness was shown to exist between -1,594 and -700 of the rat FAS promoter. Moreover, responsiveness to PRL was abolished with mutation of a site …

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Ethnicity, Education, And Blood Pressure In Cuba, Pedro Ordunez, Jose Luis Bernal Munoz, Alfredo Espinosa-Brito, Luis Carlos Silva, Richard S. Cooper Jul 2005

Ethnicity, Education, And Blood Pressure In Cuba, Pedro Ordunez, Jose Luis Bernal Munoz, Alfredo Espinosa-Brito, Luis Carlos Silva, Richard S. Cooper

Faculty Publications

The causes of variation in hypertension risk by ethnicity and educational level are not well understood. To gain further insight into this issue in a nonindustrialized country, a population-based sample of 1,667 persons aged 15-74 years was recruited in Cienfuegos, Cuba. In this 2001-2002 study, interviewers classified 29% of participants as Black or mulatto and 71% as White. Educational attainment was stratified at the median number of school years. Compared with White women, non-White women had higher blood pressures (3.0/1.7, systolic blood pressure/ diastolic blood pressure) and a higher prevalence of hypertension (24%, 95% confidence interval: 20, 28 vs. 15%, …

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Early-Stage Folding In Proteins (In Silico) Sequence-To-Structure Relation, Michal Brylinski, Leszek Konieczny, Patryk Czerwonko, Wiktor Jurkowski, Irena Roterman Jun 2005

Early-Stage Folding In Proteins (In Silico) Sequence-To-Structure Relation, Michal Brylinski, Leszek Konieczny, Patryk Czerwonko, Wiktor Jurkowski, Irena Roterman

Faculty Publications

A sequence-to-structure library has been created based on the complete PDB database. The tetrapeptide was selected as a unit representing a well-defined structural motif. Seven structural forms were introduced for structure classification. The early-stage folding conformations were used as the objects for structure analysis and classification. The degree of determinability was estimated for the sequence-to-structure and structure-to-sequence relations. Probability calculus and informational entropy were applied for quantitative estimation of the mutual relation between them. The structural motifs representing different forms of loops and bends were found to favor particular sequences in structure-to-sequence analysis. © 2005 Hindawi Publishing Corporation.

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Effects Of Leukemia Inhibitory Factor On 3t3-L1 Adipocytes, Jessica C. Hogan, Jacqueline M. Stephens Jun 2005

Effects Of Leukemia Inhibitory Factor On 3t3-L1 Adipocytes, Jessica C. Hogan, Jacqueline M. Stephens

Faculty Publications

Leukemia inhibitory factor (LIF) is a member of the gp130 cytokine family and signals through the receptor complex of gp130 and the LIF receptor (LIFR) to activate the JAK/STAT signaling cascade. Since LIF activates STATs 1 and 3 in adipocytes, we examined the effects of LIF on 3T3-L1 adipocytes. Our studies clearly demonstrate that LIF treatment had minimal effects on adipocyte differentiation as judged by marker gene expression, but did inhibit triacylglyceride (TAG) accumulation during adipogenesis. Acute treatment with LIF resulted in increased expression of suppressors of cytokine signaling-3 (SOCS3) and CCAAT/enhancer-binding protein-δ (C/EBPδ) mRNA in 3T3-L1 adipocytes. Moreover, the …

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Boron Neutron Capture Therapy Of Cancer: Current Status And Future Prospects, Rolf F. Barth, Jeffrey A. Coderre, M. Graça H. Vicente, Thomas E. Blue Jun 2005

Boron Neutron Capture Therapy Of Cancer: Current Status And Future Prospects, Rolf F. Barth, Jeffrey A. Coderre, M. Graça H. Vicente, Thomas E. Blue

Faculty Publications

Background: Boron neutron capture therapy (BNCT) is based on the nuclear reaction that occurs when boron-10 is irradiated with low-energy thermal neutrons to yield high linear energy transfer α particles and recoiling lithium-7 nuclei. Clinical interest in BNCT has focused primarily on the treatment of high-grade gliomas and either cutaneous primaries or cerebral metastases of melanoma, most recently, head and neck and liver cancer. Neutron sources for BNCT currently are limited to nuclear reactors and these are available in the United States, Japan, several European countries, and Argentina. Accelerators also can be used to produce epithermal neutrons and these are …

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Subzero Water Permeability Parameters And Optimal Freezing Rates For Sperm Cells Of The Southern Platyfish, Xiphophorus Maculatus, D. Pinisetty, C. Huang, Q. Dong, T. R. Tiersch, R. V. Devireddy Jun 2005

Subzero Water Permeability Parameters And Optimal Freezing Rates For Sperm Cells Of The Southern Platyfish, Xiphophorus Maculatus, D. Pinisetty, C. Huang, Q. Dong, T. R. Tiersch, R. V. Devireddy

Faculty Publications

This study reports the subzero water transport characteristics (and empirically determined optimal rates for freezing) of sperm cells of live-bearing fishes of the genus Xiphophorus, specifically those of the southern platyfish Xiphophorus maculatus. These fishes are valuable models for biomedical research and are commercially raised as ornamental fish for use in aquariums. Water transport during freezing of X. maculatus sperm cell suspensions was obtained using a shape-independent differential scanning calorimeter technique in the presence of extracellular ice at a cooling rate of 20°C/min in three different media: (1) Hanks' balanced salt solution (HBSS) without cryoprotective agents (CPAs); (2) HBSS with …

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Nrg1, A Cc-Nb-Lrr Protein, Together With N, A Tir-Nb-Lrr Protein, Mediates Resistance Against Tobacco Mosaic Virus, Jack R. Peart, Pere Mestre, Rui Lu, Isabelle Malcuit, David C. Baulcombe May 2005

Nrg1, A Cc-Nb-Lrr Protein, Together With N, A Tir-Nb-Lrr Protein, Mediates Resistance Against Tobacco Mosaic Virus, Jack R. Peart, Pere Mestre, Rui Lu, Isabelle Malcuit, David C. Baulcombe

Faculty Publications

In animals and plants, innate immunity is regulated by nucleotide binding domain and leucine-rich repeat (NB-LRR) proteins that mediate pathogen recognition and that activate host-cell defense responses [1, 2]. Plant NB-LRR proteins, referred to as R proteins, have amino-terminal domains that contain a coiled coil (CC) or that share similarity with animal Toll and interleukin 1 receptors (TIR) [3]. To investigate R protein function, we are using the TIR-NB-LRR protein N that mediates resistance against tobacco mosaic virus (TMV) [4] through recognition of the TMV p50 protein. Here, we describe N requirement gene 1 (NRG1), a novel N-resistance component that …

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Alu Retrotransposition-Mediated Deletion, Pauline A. Callinan, Jianxin Wang, Scott W. Herke, Randall K. Garber, Ping Liang, Mark A. Batzer May 2005

Alu Retrotransposition-Mediated Deletion, Pauline A. Callinan, Jianxin Wang, Scott W. Herke, Randall K. Garber, Ping Liang, Mark A. Batzer

Faculty Publications

Alu repeats contribute to genomic instability in primates via insertional and recombinational mutagenesis. Here, we report an analysis of Alu element-induced genomic instability through a novel mechanism termed retrotransposition-mediated deletion, and assess its impact on the integrity of primate genomes. For human and chimpanzee genomes, we find evidence of 33 retrotransposition-mediated deletion events that have eliminated approximately 9000 nucleotides of genomic DNA. Our data suggest that, during the course of primate evolution, Alu retrotransposition may have contributed to over 3000 deletion events, eliminating approximately 900 kb of DNA in the process. Potential mechanisms for the creation of Alu retrotransposition-mediated deletions …

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Phytochrome Evolution In Green And Nongreen Plants, Sarah Mathews May 2005

Phytochrome Evolution In Green And Nongreen Plants, Sarah Mathews

Faculty Publications

Photoreceptors are critical molecules that function at the interface between organism and environment. Plants use specific light signals to determine their place in time and space, allowing them to synchronize their growth, metabolism, and development to the environments in which they occur. Thus, innovation in light sensing mechanisms is expected to coincide with adaptation and diversification. Three studies involving the well-characterized phytochrome photoreceptor system in plants indicate that much work is yet needed to test this expectation. In early diverging dowering plants, episodic positive selection influenced the evolution of phytochrome A, but little of the functional data needed to link …

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Studies On The Subcellular Localization Of The Porphycene Cpo, David Kessel, Mary Conley, M. Graça H. Vicente, John J. Reiners May 2005

Studies On The Subcellular Localization Of The Porphycene Cpo, David Kessel, Mary Conley, M. Graça H. Vicente, John J. Reiners

Faculty Publications

This study was designed to provide more detailed information on the subcellular sites of binding of the porphycene, termed 9-capronyloxytetrakis (methoxyethyl) porphycene (CPO), with a fluorescence resonance energy transfer (FRET) technique. The proximity of CPO to two fluorescent probes was determined: nonyl acridine orange (NAO), a dye with specific affinity for the mitochondrial lipid cardiolipin, and dihexaoxacarbocyanine iodide (DiOC 6), an agent that labels the endoplasmic reticulum (ER). FRET spectra indicated energy transfer between DiOC 6 and CPO but no significant transfer between NAO and CPO. These results confirm data obtained by fluorescence microscopy, suggesting a similar pattern of subcellular …

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Characterization Of The Domain-Domain Interactions And Dna Binding In The Saccharomyces Cervisiae Hmcb Protein, Hmo1, Kevin Thomas Bauerle May 2005

Characterization Of The Domain-Domain Interactions And Dna Binding In The Saccharomyces Cervisiae Hmcb Protein, Hmo1, Kevin Thomas Bauerle

Honors Capstones

No abstract provided.

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Under The Genomic Radar: The Stealth Model Of Alu Amplification, Kyudong Han, Jinchuan Xing, Hui Wang, Dale J. Hedges, Randall K. Garber, Richard Cordaux, Mark A. Batzer May 2005

Under The Genomic Radar: The Stealth Model Of Alu Amplification, Kyudong Han, Jinchuan Xing, Hui Wang, Dale J. Hedges, Randall K. Garber, Richard Cordaux, Mark A. Batzer

Faculty Publications

Alu elements are the most successful SINEs (Short INterspersed Elements) in primate genomes and have reached more than 1,000,000 copies in the human genome. The amplification of most Alu elements is thought to occur through a limited number of hyperactive "master" genes that produce a high number of copies during long evolutionary periods of time. However, the existence of long-lived, low-activity Alu lineages in the human genome suggests a more complex propagation mechanism. Using both computational and wet-bench approaches, we reconstructed the evolutionary history of the AluYb lineage, one of the most active Alu lineages in the human genome. We …

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The Manganese-Stabilizing Protein Is Required For Photosystem Ii Assembly/Stability And Photoautotrophy In Higher Plants, Xiaoping Yi, Myriam Mcchargue, Susan Laborde, Laurie K. Frankel, Terry M. Bricker Apr 2005

The Manganese-Stabilizing Protein Is Required For Photosystem Ii Assembly/Stability And Photoautotrophy In Higher Plants, Xiaoping Yi, Myriam Mcchargue, Susan Laborde, Laurie K. Frankel, Terry M. Bricker

Faculty Publications

Interfering RNA was used to suppress the expression of two genes that encode the manganese-stabilizing protein of photosystem II in Arabidopsis thaliana, MSP-1 (encoded by psbO-1, At5g66570), and MSP-2 (encoded by psbO-2, At3g50820). A phenotypic series of transgenic plants was recovered that expressed high, intermediate, and low amounts of these two manganese-stabilizing proteins. Chlorophyll fluorescence induction and decay analyses were performed. Decreasing amounts of expressed protein led to the progressive loss of variable fluorescence and a marked decrease in the fluorescence quantum yield (F v/Fm) in both the absence and the presence of dichloromethylurea. This result indicated that the amount …

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High-Frequency Phase Variation Of Vibrio Vulnificus 1003: Isolation And Characterization Of A Rugose Phenotypic Variant, Brenda L. Grau, Margaret C. Henk, Gregg S. Pettis Apr 2005

High-Frequency Phase Variation Of Vibrio Vulnificus 1003: Isolation And Characterization Of A Rugose Phenotypic Variant, Brenda L. Grau, Margaret C. Henk, Gregg S. Pettis

Faculty Publications

The marine bacterium Vibrio vulnificus is a human pathogen that can spontaneously switch between virulent opaque and avirulent translucent phenotypes. Here, we document an additional form, the rugose variant, which produces copious biofilms and which may contribute both to pathogenicity of V. vulnificus and to its survival under adverse environmental conditions. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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Differential Dna Binding And Protection By Dimeric And Dodecameric Forms Of The Ferritin Homolog Dps From Deinococcus Radiodurans, Anne Grove, Steven P. Wilkinson Apr 2005

Differential Dna Binding And Protection By Dimeric And Dodecameric Forms Of The Ferritin Homolog Dps From Deinococcus Radiodurans, Anne Grove, Steven P. Wilkinson

Faculty Publications

Bacterial iron storage proteins such as ferritin serve as intracellular iron reserves. Members of the DNA protection during starvation (Dps) family of proteins are structurally related to ferritins, and their function is to protect the genome from iron-induced free radical damage. Some members of the Dps family bind DNA and are thought to do so only as fully assembled dodecamers. We present the cloning and characterization of a Dps homolog encoded by the radiation-resistant eubacterium Deinococcus radiodurans and show that DNA binding does not require its assembly into a dodecamer. D. radiodurans Dps-1, the product of gene DR2263, adopts a …

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Regionally Isolated Populations Of An Imperiled Caribbean Coral, Acropora Palmata, Iliana B. Baums, Margaret W. Miller, Michael E. Hellberg Apr 2005

Regionally Isolated Populations Of An Imperiled Caribbean Coral, Acropora Palmata, Iliana B. Baums, Margaret W. Miller, Michael E. Hellberg

Faculty Publications

The movements of larvae between marine populations are difficult to follow directly and have been the subject of much controversy, especially in the Caribbean. The debate centres on the degree to which populations are demographically open, such that depleted populations can be replenished by recruitment from distant healthy populations, or demographically closed and thus in need of local management. Given the depressed state of many tropical reef populations, the understanding of these movements now bears critically on the number, placement, and size of marine reserves. Most genetic analyses assume that dispersal patterns have been stable for thousands of generations, thus …

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Egf-Stimulation Activates The Nuclear Localization Signal Of Shp-1, Dandan He, Xi Song, Lijun Liu, David H. Burk, G. Wayne Zhou Apr 2005

Egf-Stimulation Activates The Nuclear Localization Signal Of Shp-1, Dandan He, Xi Song, Lijun Liu, David H. Burk, G. Wayne Zhou

Faculty Publications

Protein tyrosine phosphatase SHP-1 plays a critical role in the regulation of a variety of intracellular signaling pathways. SHP-1 is predominantly expressed in the cells of hematopoietic origin, and is recognized as a negative regulator of lymphocyte development and activation. SHP-1 consists of two Src homology 2 (SH2) domains and one protein tyrosine phosphatase (PTP) domain followed by a highly basic C-terminal tail containing tyrosyl phosphorylation sites. It is unclear how the C-terminal tail regulates SHP-1 function. We report the examination of the subcellular localization of a variety of truncated or mutated SHP-1 proteins fused with enhanced green fluorescent protein …

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Functional Genomics Of Carboxyltransferase, Nathan R. Ranney Apr 2005

Functional Genomics Of Carboxyltransferase, Nathan R. Ranney

Honors Capstones

No abstract provided.

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