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Improved Cryopreservation Of Sperm Of Paddlefish (Polyodon Spathula), Ákos Horváth, Béla Urbányi, Steven D. Mims, William B. Bean, Boris Gomelsky, Terrence R. Tiersch Dec 2006

Improved Cryopreservation Of Sperm Of Paddlefish (Polyodon Spathula), Ákos Horváth, Béla Urbányi, Steven D. Mims, William B. Bean, Boris Gomelsky, Terrence R. Tiersch

Faculty Publications

Experiments were performed to improve protocols for sperm cryopreservation of paddlefish (Polyodon spathula), a species for which there has been limited study. The first experiment was conducted to investigate the effects of two extenders (modified Tsvetkova's extender: mT and modified Hanks'balanced salt solution: mHBSS) in combination with methanol (MeOH) and dimethyl sulfoxide in two concentrations (5 and 10%) on the postthaw motility and fertilization rates of cryopreserved sperm. The highest postthaw motility (85 ± 5%) was observed when sperm were frozen using mT extender with 10% MeOH as cryoprotectant. Extenders (P = 0.0018) and cryoprotectants (P = 0.0040) each had …

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Emergence Of Primate Genes By Retrotransposon-Mediated Sequence Transduction, Jinchuan Xing, Hui Wang, Victoria P. Belancio, Richard Cordaux, Prescott L. Deininger, Mark A. Batzer Nov 2006

Emergence Of Primate Genes By Retrotransposon-Mediated Sequence Transduction, Jinchuan Xing, Hui Wang, Victoria P. Belancio, Richard Cordaux, Prescott L. Deininger, Mark A. Batzer

Faculty Publications

Gene duplication is one of the most important mechanisms for creating new genes and generating genomic novelty. Retrotransposon-mediated sequence transduction (i.e., the process by which a retrotransposon carries flanking sequence during its mobilization) has been proposed as a gene duplication mechanism. L1 exon shuffling potential has been reported in cell culture assays, and two potential L1-mediated exon shuffling events have been identified in the genome. SVA is the youngest retrotransposon family in primates and is capable of 3′ flanking sequence transduction during retrotransposition. In this study, we examined all of the full-length SVA elements in the human genome to assess …

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Establishment Of Cecidochares Connexa (Diptera: Tephritidae) In Guam And Its Effect On The Growth Of Chromolaena Odorata (Asteraceae), Z. T. Cruz, R. Muniappan, G. V.P. Reddy Nov 2006

Establishment Of Cecidochares Connexa (Diptera: Tephritidae) In Guam And Its Effect On The Growth Of Chromolaena Odorata (Asteraceae), Z. T. Cruz, R. Muniappan, G. V.P. Reddy

Faculty Publications

The gall fly Cecidochares connexa (Macquart) (Diptera: Tephritidae) was imported from Indone ia into Guam in 1998. It was field-established at one location in north central Guam, and its spatial and temporal spread in Guam was monitored. The biology of the gall fly is presented. No relationship between the number of larvae in a gall and the dimensions of the gall (length and width) existed. Three pupae per gall was the most frequent density with a range of 1-7. The sex ratio of flies emerging from galls was 1:1 irrespective of number of larvae per gall. C. connexa-induced galls established …

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Electrophile-Induced Ether Transfer: A New Approach To Polyketide Structural Units, Kai Liu, Richard E. Taylor, Rendy Kartika Nov 2006

Electrophile-Induced Ether Transfer: A New Approach To Polyketide Structural Units, Kai Liu, Richard E. Taylor, Rendy Kartika

Faculty Publications

A strategically novel approach to the formation of syn-1,3-diol mono- and diethers through electrophilic activation of homoallylic alkoxymethyl ethers has been developed. The resulting polyketide-like synthetic fragments are generated in good yield and with excellent stereocontrol. A chairlike transition state is proposed to account for the high stereoselectivity. Varying the conditions of the reaction workup results in the efficient generation of mono- and diether containing structural units common to polyketide natural products. © 2006 American Chemical Society.

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A Molecular Phylogenetic Analysis Of Strombid Gastropod Morphological Diversity, Jared M. Latiolais, Michael S. Taylor, Kaustuv Roy, Michael E. Hellberg Nov 2006

A Molecular Phylogenetic Analysis Of Strombid Gastropod Morphological Diversity, Jared M. Latiolais, Michael S. Taylor, Kaustuv Roy, Michael E. Hellberg

Faculty Publications

The shells of strombid gastropods show a wide variety of forms, ranging from small and fusiform to large and elaborately ornamented with a strongly flared outer lip. Here, we present the first species-level molecular phylogeny for strombids and use the resulting phylogenetic framework to explore relationships between species richness and morphological diversity. We use portions of one nuclear (325 bp of histone H3) and one mitochondrial (640 bp of cytochrome oxidase I, COI) gene to infer relationships within the two most species-rich genera in the Strombidae: Strombus and Lambis. We include 32 species of Strombus, representing 10 of 11 extant …

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Estradiol Interactions With Dopamine Antagonists In Mares: Prolactin Secretion And Reproductive Traits, K. K. Kelley, D. L. Thompson, W. A. Storer, P. B. Mitcham, R. M. Gilley, P. J. Burns Nov 2006

Estradiol Interactions With Dopamine Antagonists In Mares: Prolactin Secretion And Reproductive Traits, K. K. Kelley, D. L. Thompson, W. A. Storer, P. B. Mitcham, R. M. Gilley, P. J. Burns

Faculty Publications

Two experiments studied the effects of pretreatment with estradiol benzoate before treatment with a dopamine antagonist on prolactin secretion and reproductive traits in mares during (1) the seasonal anovulatory period and (2) the normal breeding season. Experiment 1 was performed in winter with 17 mares selected for low follicular activity. Nine mares received estradiol benzoate injections every other day for a total of 10 injections; 8 mares received similar injections of vehicle. Ten days after onset of injections, all mares were placed on daily injections of sulpiride (250 mg) for 35 days or until ovulation. Plasma prolactin concentrations were higher …

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An Organic White Light-Emitting Fluorophore, Youjun Yang, Mark Lowry, Corin M. Schowalter, Sayo O. Fakayode, Jorge O. Escobedo, Xiangyang Xu, Huating Zhang, Timothy J. Jensen, Frank R. Fronczek, Isiah M. Warner, Robert M. Strongin Nov 2006

An Organic White Light-Emitting Fluorophore, Youjun Yang, Mark Lowry, Corin M. Schowalter, Sayo O. Fakayode, Jorge O. Escobedo, Xiangyang Xu, Huating Zhang, Timothy J. Jensen, Frank R. Fronczek, Isiah M. Warner, Robert M. Strongin

Faculty Publications

The synthesis of new benzo[a]- and [b]xanthene dye frameworks is described. A unique benzo[a]xanthene, seminaphtho[a]fluorone (SNAFR-1), is studied in a variety of media. The optimization of solution parameters and excitation wavelengths allows SNAFR-1 to display red, green, and blue emission bands of approximately equal intensities and also to produce white light. Ratiometric red (anion) and green (neutral) emissions are observed upon varying solution pH. A pH-independent violet-blue emission band is due to the addition of nucleophiles to the benzylic carbon of SNAFR-1. © 2006 American Chemical Society.

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Siamese, A Plant-Specific Cell Cycle Regulator, Controls Endoreplication Onset In Arabidopsis Thaliana, Michelle L. Churchman, Matthew L. Brown, Naohiro Kato, Viktor Kirik, Martin Hülskamp, Dirk Inzé, Lieven De Veylder, Jason D. Walker, Ui Zheng, David G. Oppenheimer, Taylor Gwin, Jason Churchman, John C. Larkin Nov 2006

Siamese, A Plant-Specific Cell Cycle Regulator, Controls Endoreplication Onset In Arabidopsis Thaliana, Michelle L. Churchman, Matthew L. Brown, Naohiro Kato, Viktor Kirik, Martin Hülskamp, Dirk Inzé, Lieven De Veylder, Jason D. Walker, Ui Zheng, David G. Oppenheimer, Taylor Gwin, Jason Churchman, John C. Larkin

Faculty Publications

Recessive mutations in the SIAMESE (SIM) gene of Arabidopsis thaliana result in multicellular trichomes harboring individual nuclei with a low ploidy level, a phenotype strikingly different from that of wild-type trichomes, which are single cells with a nuclear DNA content of ∼16C to 32C. These observations suggested that SIM is required to suppress mitosis as part of the switch to endoreplication in trichomes. Here, we demonstrate that SIM encodes a nuclear-localized 14-kD protein containing a cyclin binding motif and a motif found in ICK/KRP (for interactors of Cdc2 kinase/Kip-related protein) cell cycle inhibitor proteins. Accordingly, SIM was found to associate …

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The Saccharomyces Cerevisiae Rna Polymerase Iii Recruitment Factor Subunits Brf1 And Bdp1 Impose A Strict Sequence Preference For The Downstream Half Of The Tata Box, Nick D. Tsihlis, Anne Grove Nov 2006

The Saccharomyces Cerevisiae Rna Polymerase Iii Recruitment Factor Subunits Brf1 And Bdp1 Impose A Strict Sequence Preference For The Downstream Half Of The Tata Box, Nick D. Tsihlis, Anne Grove

Faculty Publications

Association of the TATA-binding protein (TBP) with its cognate site within eukaryotic promoters is key to accurate and efficient transcriptional initiation. To achieve recruitment of Saccharomyces cerevisiae RNA polymerase III, TBP is associated with two additional factors, Brf1 and Bdp1, to form the initiation factor TFIIIB. Previous data have suggested that the structure or dynamics of the TBP-DNA complex may be altered upon entry of Brf1 and Bdp1 into the complex. We show here, using the altered specificity TBP mutant TBPm3 and an iterative in vitro selection assay, that entry of Brf1 and Bdp1 into the complex imposes a strict …

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Geographic Variation In Clonal Structure In A Reef-Building Caribbean Coral, Acropora Palmata, Iliana B. Baums, Margaret W. Miller, Michael E. Hellberg Nov 2006

Geographic Variation In Clonal Structure In A Reef-Building Caribbean Coral, Acropora Palmata, Iliana B. Baums, Margaret W. Miller, Michael E. Hellberg

Faculty Publications

Species that build the physical structure of ecosystems often reproduce clonally, both in terrestrial (e.g., grasses, trees) and marine (e.g., corals, seagrasses) environments. The degree of clonality may vary over a species' range in accordance with the relative success of sexual and asexual recruitment. High genotypic (clonal) diversity of structural species may promote the species diversity and resilience of ecosystems in the face of environmental extremes. Conversely, low genotypic diversity may indicate an asexual strategy to maintain resources and genetic variation during population decline. Here, we use microsatellite markers to assess geographic variation in clonality in the coral Acropora palmata …

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Voluntary Participation And Informed Consent International Genetic Research, Patricia A. Marshall, Clement A. Adebamowo, Adebowale A. Adeyemo, Temidayo O. Ogundiran, Mirjana Vekich, Teri Strenski, Jie Zhou, T. Elaine Prewitt, Richard S. Cooper, Charles N. Rotimi Nov 2006

Voluntary Participation And Informed Consent International Genetic Research, Patricia A. Marshall, Clement A. Adebamowo, Adebowale A. Adeyemo, Temidayo O. Ogundiran, Mirjana Vekich, Teri Strenski, Jie Zhou, T. Elaine Prewitt, Richard S. Cooper, Charles N. Rotimi

Faculty Publications

Objectives. We compared voluntary participation and comprehension of informed consent among individuals of African ancestry enrolled in similarly designed genetic studies of hypertension in the United States and Nigeria. Methods. Survey questionnaires were used to evaluate factors associated with voluntariness (the number of people volunteering) and understanding of the study's genetic purpose. A total of 655 individuals (United States: 348; Nigeria: 307) were interviewed after participation in the genetic studies. Results. Most US respondents (99%), compared with 72% of Nigerian respondents, reported being told the study purpose. Fewer than half of the respondents at both sites reported that the study …

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Common Variants In The Enpp1 Gene Are Not Reproducibly Associated With Diabetes Or Obesity, Helen N. Lyon, Jose C. Florez, Todd Bersaglieri, Richa Saxena, Wendy Winckler, Peter Almgren, Ulf Lindblad, Tiinamaija Tuomi, Daniel Gaudet, Xiaofeng Zhu, Richard Cooper, Kristin G. Ardlie, Mark J. Daly, David Altshuler, Leif Groop, Joel N. Hirschhorn Nov 2006

Common Variants In The Enpp1 Gene Are Not Reproducibly Associated With Diabetes Or Obesity, Helen N. Lyon, Jose C. Florez, Todd Bersaglieri, Richa Saxena, Wendy Winckler, Peter Almgren, Ulf Lindblad, Tiinamaija Tuomi, Daniel Gaudet, Xiaofeng Zhu, Richard Cooper, Kristin G. Ardlie, Mark J. Daly, David Altshuler, Leif Groop, Joel N. Hirschhorn

Faculty Publications

The common missense single nucleotide polymorphism (SNP) K121Q in the ectoenzyme nucleotide pyrophosphate phosphodiesterase (ENPP1) gene has recently been associated with type 2 diabetes in Italian, U.S., and South-Asian populations. A three-SNP haplotype, including K121Q, has also been associated with obesity and type 2 diabetes in French and Austrian populations. We set out to confirm these findings in several large samples. We genotyped the haplotype K121Q (rs1044498), rs1799774, and rs7754561 in 8,676 individuals of European ancestry with and without type 2 diabetes, in 1,900 obese and 930 lean individuals of European ancestry from the U.S. and Poland, and in 1,101 …

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Purification And Preconcentration Of Genomic Dna From Whole Cell Lysates Using Photoactivated Polycarbonate (Ppc) Microfluidic Chips, Małgorzata A. Witek, Shawn D. Llopis, Abigail Wheatley, Robin L. Mccarley, Steven A. Soper Oct 2006

Purification And Preconcentration Of Genomic Dna From Whole Cell Lysates Using Photoactivated Polycarbonate (Ppc) Microfluidic Chips, Małgorzata A. Witek, Shawn D. Llopis, Abigail Wheatley, Robin L. Mccarley, Steven A. Soper

Faculty Publications

We discuss the use of a photoactivated polycarbonate (PPC) microfluidic chip for the solid-phase, reversible immobilization (SPRI) and purification of genomic DNA (gDNA) from whole cell lysates. The surface of polycarbonate was activated by UV radiation resulting in a photo-oxidation reaction, which produced a channel surface containing carboxylate groups. The gDNA was selectively captured on this photoactivated surface in an immobilization buffer, which consisted of 3% polyethylene glycol, 0.4 M NaCl and 70% ethanol. The methodology reported herein is similar to conventional SPRI in that surface-confined carboxylate groups are used for the selective immobilization of DNA; however, no magnetic beads …

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Spatial Confidentiality And Gis: Re-Engineering Mortality Locations From Published Maps About Hurricane Katrina, Andrew J. Curtis, Jacqueline W. Mills, Michael Leitner Oct 2006

Spatial Confidentiality And Gis: Re-Engineering Mortality Locations From Published Maps About Hurricane Katrina, Andrew J. Curtis, Jacqueline W. Mills, Michael Leitner

Faculty Publications

Background: Geographic Information System (GIS) can provide valuable insight into patterns of human activity. Online spatial display applications, such as Google Earth, can democratise this information by disseminating it to the general public. Although this is a generally positive advance for society, there is a legitimate concern involving the disclosure of confidential information through spatial display. Although guidelines exist for aggregated data, little has been written concerning the display of point level information. The concern is that a map containing points representing cases of cancer or an infectious disease, could be re-engineered back to identify an actual residence. This is …

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Liana Habitat Associations And Community Structure In A Bornean Lowland Tropical Forest, Saara J. Dewalt, Kalan Ickes, Reuben Nilus, Kyle E. Harms, David F.R.P. Burslem Oct 2006

Liana Habitat Associations And Community Structure In A Bornean Lowland Tropical Forest, Saara J. Dewalt, Kalan Ickes, Reuben Nilus, Kyle E. Harms, David F.R.P. Burslem

Faculty Publications

Lianas (woody vines) contribute substantially to the diversity and structure of most tropical forests, yet little is known about the importance of habitat specialization in maintaining tropical liana diversity and the causes of variation among forests in liana abundance and species composition. We examined habitat associations, species diversity, species composition, and community structure of lianas at Sepilok Forest Reserve, Sabah, Malaysia in northeastern Borneo among three soil types that give rise to three distinct forest types of lowland tropical rain forest: alluvial, sandstone hill, and kerangas (heath) forest. Alluvial soils are more nutrient rich and have higher soil moisture than …

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Louisiana Agriculture Fall, 2006, Lsu Agcenter Oct 2006

Louisiana Agriculture Fall, 2006, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

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Phytochrome-Mediated Development In Land Plants: Red Light Sensing Evolves To Meet The Challenges Of Changing Light Environments, Sarah Mathews Oct 2006

Phytochrome-Mediated Development In Land Plants: Red Light Sensing Evolves To Meet The Challenges Of Changing Light Environments, Sarah Mathews

Faculty Publications

Phytochromes are photoreceptors that provide plants with circadian, seasonal, and positional information critical for the control of germination, seedling development, shade avoidance, reproduction, dormancy, and sleep movements. Phytochromes are unique among photoreceptors in their capacity to interconvert between a red-absorbing form (absorption maximum of ∼660 nm) and a far-red absorbing form (absorption maximum of ∼730 nm), which occur in a dynamic equilibrium within plant cells, corresponding to the proportions of red and far-red energy in ambient light. Because pigments in stems and leaves absorb wavelengths below about 700 nm, this provides plants with an elegant system for detecting their position …

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Thermal Stability Landscape For Klenow Dna Polymerase As A Function Of Ph And Salt Concentration, Allison J. Richard, Chin Chi Liu, Alexandra L. Klinger, Matthew J. Todd, Tara M. Mezzasalma, Vince J. Licata Oct 2006

Thermal Stability Landscape For Klenow Dna Polymerase As A Function Of Ph And Salt Concentration, Allison J. Richard, Chin Chi Liu, Alexandra L. Klinger, Matthew J. Todd, Tara M. Mezzasalma, Vince J. Licata

Faculty Publications

The thermal denaturation of Klenow DNA polymerase has been characterized over a wide variety of solution conditions to obtain a relative stability landscape for the protein. Measurements were conducted utilizing a miniaturized fluorescence assay that measures Tm based on the increase in the fluorescence of 1,8-anilinonaphthalene sulfonate (ANS) when the protein denatures. The melting temperature (Tm) for Klenow increases as the salt concentration is increased and as the pH is decreased. Klenow's Tm spans a range of over 20 °C, from 40 to 62 °C, depending upon the solution conditions. The landscape reconciles and extends previously measured Tm values for …

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Integrin Structures And Conformational Signaling, Bing Hao Luo, Timothy A. Springer Oct 2006

Integrin Structures And Conformational Signaling, Bing Hao Luo, Timothy A. Springer

Faculty Publications

Integrins are cell adhesion molecules that play critical roles in development, wound healing, hemostasis, immunity and cancer. Advances in the past two years have shed light on the structural basis for integrin regulation and signaling, especially on how global conformational changes between bent and extended conformations relate to the inter-domain and intra-domain shape shifting that regulates affinity for ligand. The downward movements of the C-terminal helices of the α I and β I domains and the swing-out of the hybrid domain play pivotal roles in integrin conformational signaling. Experiments have also shown that integrins transmit bidirectional signals across the plasma …

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Interplay Of Isca And Iscu In Biogenesis Of Iron-Sulfur Clusters, Juanjuan Yang, Jacob P. Bitoun, Huangen Ding Sep 2006

Interplay Of Isca And Iscu In Biogenesis Of Iron-Sulfur Clusters, Juanjuan Yang, Jacob P. Bitoun, Huangen Ding

Faculty Publications

Increasing evidence suggests that sulfur in ubiquitous iron-sulfur clusters is derived from L-cysteine via cysteine desulfurases. In Escherichia coli, the major cysteine desulfurase activity for biogenesis of iron-sulfur clusters has been attributed to IscS. The gene that encodes IscS is a member of an operon isc-SUA, which also encodes two highly conserved proteins: IscU and IscA. Previous studies suggested that both IscU and IscA may act as the iron-sulfur cluster assembly scaffold proteins. However, recent evidence indicated that IscA is an iron-binding protein that can provide iron for the iron-sulfur cluster assembly in IscU (Ding, H., Harrison, K., and Lu, …

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Effects Of Rice Panicle Age On Quantitative And Qualitative Injury By The Rice Stink Bug (Hemiptera: Pentatomidae), Dilipkumar T. Patel, Michael J. Stout, James R. Fuxa Sep 2006

Effects Of Rice Panicle Age On Quantitative And Qualitative Injury By The Rice Stink Bug (Hemiptera: Pentatomidae), Dilipkumar T. Patel, Michael J. Stout, James R. Fuxa

Faculty Publications

Greenhouse experiments were conducted to evaluate the effects of panicle age on quantitative and qualitative injury caused by rice stink bug, Oebalus pugnax pugnax (Fab.), infestations on rice, Oryza sativa L. The effects were measured at two infestation levels (one and two bugs per panicle) and compared with an undamaged control. Percentage of empty grains and average weight of filled grains (quantitative injury) and percentage of pecky rice (qualitative injury) were evaluated at grain maturity. Regardless of infestation level, insect feeding during anthesis and the early milk stage of grain development (first 8 d after anthesis) caused substantially higher numbers …

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Adult Phyllophaga Ephilida Host Plant Feeding Preference, Aboubacar Diagne, Richard N. Story, Abner M. Hammond Sep 2006

Adult Phyllophaga Ephilida Host Plant Feeding Preference, Aboubacar Diagne, Richard N. Story, Abner M. Hammond

Faculty Publications

Limited biological information about Phyllophaga ephilida, a major sweet potato pest in Louisiana, is available. In 2001 and 2002, a study was conducted in the laboratory to investigate the feeding preference of adult Phyllophaga ephilida (Say) (Coleoptera: Scarabaeidae) for the foliage of eight woody plant species: water oak (Quercus nigra L.), live oak (Quercus virginiana Mill.), red maple (Acer rubrum L.), slash pine (Pinus caribaea Morelet), pecan (Carya illinoensis (Wangenh) K. Koch), sweetgum (Liquidambar styraciflua L.), southern magnolia (Magnolia grandiflora L.), and American elm (Ulmus americana L.). Beetles were placed in an arena with the eight host plants and allowed …

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Mesostructured Silica Chemically Doped With RuIi As A Superior Optical Oxygen Sensor, Bingfu Lei, Bin Li, Haoran Zhang, Shaozhe Lu, Zhuhong Zheng, Wenlian Li, Yue Wang Sep 2006

Mesostructured Silica Chemically Doped With RuIi As A Superior Optical Oxygen Sensor, Bingfu Lei, Bin Li, Haoran Zhang, Shaozhe Lu, Zhuhong Zheng, Wenlian Li, Yue Wang

Faculty Publications

Novel oxygen sensors consisting of a [Ru(bpy)2phen]2+ (bpy: 2,2′-bipyridyl, phen: phenathroline) portion covalently grafted to a mesostructured silica-based network are prepared in situ via a sol-gel approach with the help of cetyltrimethylammonium-bromide (CTAB) surfactant. 1,10-Phenanthroline covalently grafted to 3-(triethoxysilyl)propyl isocyanate is used as not only the sol-gel precursor but also as the second ligand of the Ru(bpy)2d2 · 2H2O complex to prepare the sol-gel-derived mesostructured silicates for an oxygen sensor. For comparison purposes, the oxygen sensors in which [Ru(bpy)2phen]Q2 is conventionally physically incorporated into the matrix are also prepared. Elemental analysis, NMR, Fourier transform IR, UV-vis electronic absorption, luminescence-intensity quenching …

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The Psbq Protein Is Required In Arabidopsis For Photosystem Ii Assembly/Stability And Photoautotrophy Under Low Light Conditions, Xiaoping Yi, Stefan R. Hargett, Laurie K. Frankel, Terry M. Bricker Sep 2006

The Psbq Protein Is Required In Arabidopsis For Photosystem Ii Assembly/Stability And Photoautotrophy Under Low Light Conditions, Xiaoping Yi, Stefan R. Hargett, Laurie K. Frankel, Terry M. Bricker

Faculty Publications

RNA interference was used to simultaneously suppress the expression of the two genes that encode the PsbQ proteins of Photosystem II (PS II) in Arabidopsis thaliana, psbQ-1 (At4g21280) and psbQ-2 (At4g05180). Two independent PsbQ-deficient plant lines were examined. These plant lines produced little detectable PsbQ protein. Under normal growth light conditions, the wild type and mutant plants were visually indistinguishable. Additionally, analysis of steady state oxygen evolution rates and chlorophyll fluorescence characteristics indicated little alteration of photosynthetic capacity in the mutant plants. No loss of other PS II proteins was evident. Interestingly, flash oxygen yield analysis performed on thylakoid membranes …

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Post-Thaw Amendment Of Cryopreserved Sperm For Use In Artificial Insemination Of A Viviparous Fish, The Green Swordtail Xiphophorus Helleri, Qiaoxiang Dong, Changjiang Huang, Terrence R. Tiersch Sep 2006

Post-Thaw Amendment Of Cryopreserved Sperm For Use In Artificial Insemination Of A Viviparous Fish, The Green Swordtail Xiphophorus Helleri, Qiaoxiang Dong, Changjiang Huang, Terrence R. Tiersch

Faculty Publications

Sperm cryopreservation protocols have been developed for live-bearers such as the green swordtail Xiphophorus helleri and the platyfish Xiphophorus couchianus. Despite the high post-thaw motility (∼ 75%) obtained in both species, the requirements of sperm storage within the female reproductive tract coupled with the process of internal fertilization place functional demands upon cryopreserved sperm samples far beyond those of oviparous species. The purpose of this study was to facilitate the artificial insemination process with cryopreserved sperm of X. helleri through evaluation of parameters related to sperm quality after thawing. Specifically, this study evaluated the effects on motility for fresh and …

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Proteomic And Genetic Approaches To Identifying Defence-Related Proteins In Rice Challenged With The Fungal Pathogen Rhizoctonia Solani, Joohyun Lee, Terry M. Bricker, Michael Lefevre, Shannon R.M. Pinson, James H. Oard Sep 2006

Proteomic And Genetic Approaches To Identifying Defence-Related Proteins In Rice Challenged With The Fungal Pathogen Rhizoctonia Solani, Joohyun Lee, Terry M. Bricker, Michael Lefevre, Shannon R.M. Pinson, James H. Oard

Faculty Publications

Sheath blight, caused by the fungus Rhizoctonia solani, is a major disease of rice world-wide, but little is known about the host response to infection. The objective of this study was to identify proteins and DNA markers in resistant and susceptible rice associated with response to infection by R. solani. Replicated two-dimensional polyacrylamide gel electrophoresis experiments were conducted to detect proteins differentially expressed under inoculated and non-inoculated conditions. Tandem mass spectra analysis using electrospray ionization quadrupole-time of flight mass spectrometry (ESI Q-TOF MS) was carried out for protein identification with the NCBI non-redundant protein database. Seven proteins were increased after …

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Tc-99m Pyrophosphate Imaging Of Poloxamer-Treated Electroporated Skeletal Muscle In An In Vivo Rat Model, Kenneth L. Matthews, John N. Aarsvold, Robert A. Mintzer, Chin Tu Chen, Raphael C. Lee Sep 2006

Tc-99m Pyrophosphate Imaging Of Poloxamer-Treated Electroporated Skeletal Muscle In An In Vivo Rat Model, Kenneth L. Matthews, John N. Aarsvold, Robert A. Mintzer, Chin Tu Chen, Raphael C. Lee

Faculty Publications

Objective: This study investigates whether 99mTc pyrophosphate (PYP) imaging provides a quantitative non-invasive assessment of the extent of electroporation injury, and of the effect of poloxamer in vivo on electroporated skeletal muscle. Methods: High-voltage electrical shock was used to produce electroporation injury in an anesthetized rat's hind limb. In each experiment, the injured limb was treated intravenously by either poloxamer-188, dextran, or saline, and subsequently imaged with 99mTc PYP. The radiotracer's temporal behavior among the experimental groups was compared using curve fitting of time-activity curves from the dynamic image data. Results: The washout kinetics of 99mTc PYP changed in proportion …

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Tc-99m Pyrophosphate Imaging Of Poloxamer-Treated Electroporated Skeletal Muscle In An In Vivo Rat Model, Kenneth L. Matthews, John N. Aarsvold, Robert A. Mintzer, Chin Tu Chen, Raphael C. Lee Sep 2006

Tc-99m Pyrophosphate Imaging Of Poloxamer-Treated Electroporated Skeletal Muscle In An In Vivo Rat Model, Kenneth L. Matthews, John N. Aarsvold, Robert A. Mintzer, Chin Tu Chen, Raphael C. Lee

Faculty Publications

Objective: This study investigates whether 99mTc pyrophosphate (PYP) imaging provides a quantitative non-invasive assessment of the extent of electroporation injury, and of the effect of poloxamer in vivo on electroporated skeletal muscle. Methods: High-voltage electrical shock was used to produce electroporation injury in an anesthetized rat's hind limb. In each experiment, the injured limb was treated intravenously by either poloxamer-188, dextran, or saline, and subsequently imaged with 99mTc PYP. The radiotracer's temporal behavior among the experimental groups was compared using curve fitting of time-activity curves from the dynamic image data. Results: The washout kinetics of 99mTc PYP changed in proportion …

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An Improved Cloning Strategy For Chromatin-Immunoprecipitation-Derived Dna Fragments, Jennifer M. Huang, Jeong Do Kim, Hana Kim, Joomyeong Kim Sep 2006

An Improved Cloning Strategy For Chromatin-Immunoprecipitation-Derived Dna Fragments, Jennifer M. Huang, Jeong Do Kim, Hana Kim, Joomyeong Kim

Faculty Publications

No abstract provided.

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Quantifying Sulfate Components And Their Variations In Soils Of The Mcmurdo Dry Valleys, Antarctica, Huiming Bao, David R. Marchant Aug 2006

Quantifying Sulfate Components And Their Variations In Soils Of The Mcmurdo Dry Valleys, Antarctica, Huiming Bao, David R. Marchant

Faculty Publications

Many soils of the McMurdo Dry Valleys (MDV), Antarctica, being old, hyperarid, and frigid, have accumulated abundant atmospheric salts over the last several million years. This salt repository offers an opportunity to study atmospheric chemistry (past and present), the origin and transport of ions in soils, the weathering activity of soils, and postdepositional soil-leaching processes within this unique environment. In particular, soil sulfate in the MDV is known to have multiple origins, but the precise proportions of different sulfate components remain elusive. Here we test a hypothesis that soil sulfate in the MDV is a predictable mixture of three major …

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