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Arabidopsis Actin-Related Protein Arp5 In Multicellular Development And Dna Repair, Muthugapatti K. Kandasamy, Elizabeth C. Mckinney, Roger B. Deal, Aaron P. Smith, Richard B. Meagher Nov 2009

Arabidopsis Actin-Related Protein Arp5 In Multicellular Development And Dna Repair, Muthugapatti K. Kandasamy, Elizabeth C. Mckinney, Roger B. Deal, Aaron P. Smith, Richard B. Meagher

Faculty Publications

Actin-related protein 5 (ARP5) is a conserved subunit of the INO80 chromatin-remodeling complex in yeast and mammals. We have characterized the expression and subcellular distribution of Arabidopsis thaliana ARP5 and explored its role in the epigenetic control of multicellular development and DNA repair. ARP5-specific monoclonal antibodies localized ARP5 protein to the nucleoplasm of interphase cells in Arabidopsis and Nicotiana tabacum. ARP5 promoter-reporter fusions and the ARP5 protein are ubiquitously expressed. A null mutant and a severe knockdown allele produced moderately dwarfed plants with all organs smaller than the wild type. The small and slightly deformed organs such as leaves and …

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Permanent Genetic Resources Added To Molecular Ecology Resources Database 1 May 2009-31 July 2009, Glenn R. Almany, Maurício P. De Arruda, Wolfgang Arthofer, Z. K. Atallah, Steven R. Beissinger, Michael L. Berumen, S. M. Bogdanowicz, S. D. Brown, Michael W. Bruford, C. Burdine, Jeremiah W. Busch, Nathan R. Campbell, D. Carey, Bryan C. Carstens, K. H. Chu, Marc A. Cubeta, J. P. Cuda, Zhaoxia Cui, L. E. Datnoff, J. A. Dávila, Emily S. Davis, R. M. Davis, Onno E. Diekmann, Eduardo Eizirik, J. A. Fargallo, Fabiano Fernandes, Hideo Fukuda, L. R. Gale, Elizabeth Gallagher, Yongqiang Gao, Philippe Girard, Anna Godhe, Evonnildo C. Gonçalves Nov 2009

Permanent Genetic Resources Added To Molecular Ecology Resources Database 1 May 2009-31 July 2009, Glenn R. Almany, Maurício P. De Arruda, Wolfgang Arthofer, Z. K. Atallah, Steven R. Beissinger, Michael L. Berumen, S. M. Bogdanowicz, S. D. Brown, Michael W. Bruford, C. Burdine, Jeremiah W. Busch, Nathan R. Campbell, D. Carey, Bryan C. Carstens, K. H. Chu, Marc A. Cubeta, J. P. Cuda, Zhaoxia Cui, L. E. Datnoff, J. A. Dávila, Emily S. Davis, R. M. Davis, Onno E. Diekmann, Eduardo Eizirik, J. A. Fargallo, Fabiano Fernandes, Hideo Fukuda, L. R. Gale, Elizabeth Gallagher, Yongqiang Gao, Philippe Girard, Anna Godhe, Evonnildo C. Gonçalves

Faculty Publications

This article documents the addition of 512 microsatellite marker loci and nine pairs of Single Nucleotide Polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Alcippe morrisonia morrisonia, Bashania fangiana, Bashania fargesii, Chaetodon vagabundus, Colletes floralis, Coluber constrictor flaviventris, Coptotermes gestroi, Crotophaga major, Cyprinella lutrensis, Danaus plexippus, Fagus grandifolia, Falco tinnunculus, Fletcherimyia fletcheri, Hydrilla verticillata, Laterallus jamaicensis coturniculus, Leavenworthia alabamica, Marmosops incanus, Miichthys miiuy, Nasua nasua, Noturus exilis, Odontesthes bonariensis, Quadrula fragosa, Pinctada maxima, Pseudaletia separata, Pseudoperonospora cubensis, Podocarpus elatus, Portunus trituberculatus, Rhagoletis cerasi, Rhinella schneideri, Sarracenia alata, Skeletonema marinoi, Sminthurus viridis, …

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Reading Between The Lines To See Into The Past, David A. Ray, Roy N. Platt, Mark A. Batzer Nov 2009

Reading Between The Lines To See Into The Past, David A. Ray, Roy N. Platt, Mark A. Batzer

Faculty Publications

Transposable elements (TEs) are an important source of genome diversity and play a crucial role in genome evolution. A recent study by Zhao et al. describes novel patterns of TE diversification in the genome of the extinct mammoth Mammuthus primigenius. Analysis of Mammuthus has provided a unique genome landscape, a pivotal species for understanding TEs and genome evolution and hints at the diversity we verge on discovering by expanding our taxonomic sampling among genomes. Strategies based on this work might also revolutionize investigations of the interface between TE dynamics and genome diversity. © 2009 Elsevier Ltd. All rights reserved.

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5́-Transducing Sva Retrotransposon Groups Spread Efficiently Throughout The Human Genome, Annette Damert, Julija Raiz, Axel V. Horn, Johannes Löwer, Hui Wang, Jinchuan Xing, Mark A. Batzer, Roswitha Löwer, Gerald G. Schumann Nov 2009

5́-Transducing Sva Retrotransposon Groups Spread Efficiently Throughout The Human Genome, Annette Damert, Julija Raiz, Axel V. Horn, Johannes Löwer, Hui Wang, Jinchuan Xing, Mark A. Batzer, Roswitha Löwer, Gerald G. Schumann

Faculty Publications

SVA elements represent the youngest family of hominid non-LTR retrotransposons, which alter the human genome continuously. They stand out due to their organization as composite repetitive elements. To draw conclusions on the assembly process that led to the current organization of SVA elements and on their transcriptional regulation, we initiated our study by assessing differences in structures of the 116 SVA elements located on human chromosome 19. We classified SVA elements into seven structural variants, including novel variants like 3′-truncated elements and elements with 5′-flanking sequence transductions. We established a genome-wide inventory of 5′-transduced SVA elements encompassing ∼8% of all …

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Timing Of Migration And Patterns Of Winter Settlement By Henslow's Sparrows, Erik I. Johnson, Jennifer K. Dimiceli, Philip C. Stouffer Nov 2009

Timing Of Migration And Patterns Of Winter Settlement By Henslow's Sparrows, Erik I. Johnson, Jennifer K. Dimiceli, Philip C. Stouffer

Faculty Publications

Fall arrival is an important period in the life history of migratory birds because processes during this period determine where birds spend the winter, which is linked to subsequent survival and condition at the time of spring migration. Henslow's Sparrows (Ammodramus henslowii) winter in savannas of longleaf pine (Pinus palustris), but their secretive behavior limits our knowledge of their winter ecology, including behavioral processes that lead to their documented preference of an ephemeral habitat-recently burned savannas. We expected that upon arrival Henslow's Sparrows actively seek out recently burned savannas and that their over-winter location is dependent on intraspecific interactions during …

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Magnetic And Nonmagnetic Nanoparticles From A Group Of Uniform Materials Based On Organic Salts, Aaron Tesfai, Bilal El-Zahab, Algernon T. Kelley, Min Li, Jayne C. Garno, Gary A. Baker, Isiah M. Warner Oct 2009

Magnetic And Nonmagnetic Nanoparticles From A Group Of Uniform Materials Based On Organic Salts, Aaron Tesfai, Bilal El-Zahab, Algernon T. Kelley, Min Li, Jayne C. Garno, Gary A. Baker, Isiah M. Warner

Faculty Publications

The size and uniformity of magnetic nanoparticles developed from a group of uniform materials based on organic salts (GUMBOS) were controlled using an in situ ion exchange, water-in-oil (w/o) microemulsion preparation. Most of these nanoGUMBOS are in fact ionic liquids (i.e., melting points less than 100 °C), while others have melting points above the conventional 100 °C demarcation. Simple variations in the reagent concentrations following a w/o approach allowed us to smoothly and predictably vary nanoparticle dimensions across a significant size regime with excellent uniformity. Average sizesofGUMBOS particles ranging from 14 to 198 nm were achieved by manipulation of the …

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Cyanochromes Are Blue/Green Light Photoreversible Photoreceptors Defined By A Stable Double Cysteine Linkage To A Phycoviolobilin-Type Chromophore, Andrew T. Ulijasz, Gabriel Cornilescu, David Von Stetten, Claudia Cornilescu, Francisco Velazquez Escobar, Junrui Zhang, Robert J. Stankey, Mario Rivera, Peter Hildebrandt, Richard D. Vierstra Oct 2009

Cyanochromes Are Blue/Green Light Photoreversible Photoreceptors Defined By A Stable Double Cysteine Linkage To A Phycoviolobilin-Type Chromophore, Andrew T. Ulijasz, Gabriel Cornilescu, David Von Stetten, Claudia Cornilescu, Francisco Velazquez Escobar, Junrui Zhang, Robert J. Stankey, Mario Rivera, Peter Hildebrandt, Richard D. Vierstra

Faculty Publications

Phytochromes are a collection of bilin-containing photoreceptors that regulate a diverse array of processes in microorganisms and plants through photoconversion between two stable states, a red light-absorbing Pr form, and a far red light-absorbing Pfr form. Recently, a novel set of phytochrome-like chromoproteins was discovered in cyanobacteria, designated here as cyanochromes, that instead photoconvert between stable blue and green light-absorbing forms Pb and Pg, respectively. Here, we show that the distinctive absorption properties of cyanochromes are facilitated through the binding of phycocyanobilin via two stable cysteine-based thioether linkages within the cGMP phosphodiesterase/ adenyl cyclase/FhlA domain. Absorption, resonance Raman and infrared …

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Knockdown Of The Drosophila Gtpase Nucleostemin 1 Impairs Large Ribosomal Subunit Biogenesis, Cell Growth, And Midgut Precursor Cell Maintenance, Raphyel Rosby, Cui Zhengfang, Emily Rogers, Megan A. Delivron, Victoria L. Robinson, Patrick J. Dimario Oct 2009

Knockdown Of The Drosophila Gtpase Nucleostemin 1 Impairs Large Ribosomal Subunit Biogenesis, Cell Growth, And Midgut Precursor Cell Maintenance, Raphyel Rosby, Cui Zhengfang, Emily Rogers, Megan A. Delivron, Victoria L. Robinson, Patrick J. Dimario

Faculty Publications

Mammalian nucleostemin (NS) is a nucleolar guanosine triphosphate-binding protein implicated in cell cycle progression, stem cell proliferation, and ribosome assembly. Drosophila melanogaster contains a four-member nucleostemin family (NS1-4). NS1 is the closest orthologue to human NS; it shares 33% identity and 67% similarity with human NS. We show that NS1 has intrinsic GTPase and ATPase activity and that it is present within nucleoli of most larval and adult cells. Endogenous NS1 and lightly expressed green fluorescent protein (GFP)-NS1 enrich within the nucleolar granular regions as expected, whereas overexpressed GFP-NS1 localized throughout the nucleolus and nucleoplasm, and to several transcriptionally active …

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St. John's Wort Inhibits Adipocyte Differentiation And Induces Insulin Resistance In Adipocytes, Zhaleh Amini, Bryant Boyd, Julie Doucet, David M. Ribnicky, Jacqueline M. Stephens Oct 2009

St. John's Wort Inhibits Adipocyte Differentiation And Induces Insulin Resistance In Adipocytes, Zhaleh Amini, Bryant Boyd, Julie Doucet, David M. Ribnicky, Jacqueline M. Stephens

Faculty Publications

Adipocytes are insulin sensitive cells that play a major role in energy homeostasis. Obesity is the primary disease of fat cells and a major risk factor for the development of Type II diabetes, cardiovascular disease, and metabolic syndrome. Obesity and its related disorders result in dysregulation of the mechanisms that control adipocyte gene expression and function. To identify potential novel therapeutic modulators of adipocytes, we screened 425 botanical extracts for their ability to modulate adipogenesis and insulin sensitivity. We observed that less than 2% of the extracts had substantial effects on adipocyte differentiation of 3T3-L1 cells. Two of the botanical …

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Integrating Elf4 Into The Circadian System Through Combined Structural And Functional Studies, Elsebeth Kolmos, Monika Nowak, Maria Werner, Katrin Fischer, Guenter Schwarz, Sarah Mathews, Heiko Schoof, Ferenc Nagy, Janusz M. Bujnicki, Seth J. Davis Oct 2009

Integrating Elf4 Into The Circadian System Through Combined Structural And Functional Studies, Elsebeth Kolmos, Monika Nowak, Maria Werner, Katrin Fischer, Guenter Schwarz, Sarah Mathews, Heiko Schoof, Ferenc Nagy, Janusz M. Bujnicki, Seth J. Davis

Faculty Publications

The circadian clock is a timekeeping mechanism that enables anticipation of daily environmental changes. In the plant Arabidopsis thaliana, the circadian system is a multiloop series of interlocked transcription-translation feedbacks. Several genes have been arranged in these oscillation loops, but the position of the core-clock gene ELF4 in this network was previously undetermined. ELF4 lacks sequence similarity to known domains, and functional homologs have not yet been identified. Here we show that ELF4 is functionally conserved within a subclade of related sequences, and forms an alpha-helical homodimer with a likely electrostatic interface that could be structurally modeled. We support this …

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The Impact Of Retrotransposons On Human Genome Evolution, Richard Cordaux, Mark A. Batzer Oct 2009

The Impact Of Retrotransposons On Human Genome Evolution, Richard Cordaux, Mark A. Batzer

Faculty Publications

Their ability to move within genomes gives transposable elements an intrinsic propensity to affect genome evolution. Non-long terminal repeat (LTR) retrotransposons including LINE-1, Alu and SVA elements have proliferated over the past 80 million years of primate evolution and now account for approximately one-third of the human genome. In this Review, we focus on this major class of elements and discuss the many ways that they affect the human genome: from generating insertion mutations and genomic instability to altering gene expression and contributing to genetic innovation. Increasingly detailed analyses of human and other primate genomes are revealing the scale and …

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Louisiana Agriculture Fall, 2009, Lsu Agcenter Oct 2009

Louisiana Agriculture Fall, 2009, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

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Encoding Properties Of Auditory Neurons In The Brain Of A Soniferous Damselfish: Response To Simple Tones And Complex Conspecific Signals, Karen P. Maruska, Timothy C. Tricas Oct 2009

Encoding Properties Of Auditory Neurons In The Brain Of A Soniferous Damselfish: Response To Simple Tones And Complex Conspecific Signals, Karen P. Maruska, Timothy C. Tricas

Faculty Publications

The fish auditory system encodes important acoustic stimuli used in social communication, but few studies have examined response properties of central auditory neurons to natural signals. We determined the features and responses of single hindbrain and midbrain auditory neurons to tone bursts and playbacks of conspecific sounds in the soniferous damselfish, Abudefduf abdominalis. Most auditory neurons were either silent or had slow irregular resting discharge rates ~130 Hz but ranged from 80 to 400 Hz with strong phase-locking. This low-frequency sensitivity matches the frequency band of natural sounds. Auditory neurons were also modulated by playbacks of conspecific sounds with thresholds …

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Copper Oxide Nanoparticles Induce Oxidative Stress And Cytotoxicity In Airway Epithelial Cells, Baher Fahmy, Stephania A. Cormier Oct 2009

Copper Oxide Nanoparticles Induce Oxidative Stress And Cytotoxicity In Airway Epithelial Cells, Baher Fahmy, Stephania A. Cormier

Faculty Publications

Metal oxide nanoparticles are often used as industrial catalysts and elevated levels of these particles have been clearly demonstrated at sites surrounding factories. To date, limited toxicity data on metal oxide nanoparticles are available. To understand the impact of these airborne pollutants on the respiratory system, airway epithelial (HEp-2) cells were exposed to increasing doses of silicon oxide (SiO2), ferric oxide (Fe2O3) and copper oxide (CuO) nanoparticles, the leading metal oxides found in ambient air surrounding factories. CuO induced the greatest amount of cytotoxicity in a dose-dependent manner; while even high doses (400 μg/cm2) of SiO2 and Fe2O3 were non-toxic …

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Tarnished Plant Bug (Hemiptera: Miridae) Thresholds And Sampling Comparisons For Flowering Cotton In The Midsouthern United States, Fred R. Musser, Angus L. Catchot, Scott D. Stewart, Ralph D. Bagwell, Gus M. Lorenz, Kelly V. Tindall, Glenn E. Studebaker, B. Rogers Leonard, D. Scott Akin, Donald R. Cook, Chris A. Daves Oct 2009

Tarnished Plant Bug (Hemiptera: Miridae) Thresholds And Sampling Comparisons For Flowering Cotton In The Midsouthern United States, Fred R. Musser, Angus L. Catchot, Scott D. Stewart, Ralph D. Bagwell, Gus M. Lorenz, Kelly V. Tindall, Glenn E. Studebaker, B. Rogers Leonard, D. Scott Akin, Donald R. Cook, Chris A. Daves

Faculty Publications

The tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae), has become the primary target of foliar insecticides in cotton, Gossypium hirsutum L., throughout the Midsouth over the past several years. This prompted a reevaluation of existing action thresholds for flowering cotton under current production practices and economics. A trial was conducted at 19 locations throughout the Midsouth during 2006 and 2007. Threshold treatments ranged from a weekly automatic insecticide application to a very high threshold of 10 tarnished plant bugs per 1.5 row-m on a black drop cloth. Individually, all locations reached the lowest threshold, and eight locations …

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On Neolepisorus Emeiensis And N. Dengii (Polypodiaceae) From China, Xiao Si Guo, Bin Li Oct 2009

On Neolepisorus Emeiensis And N. Dengii (Polypodiaceae) From China, Xiao Si Guo, Bin Li

Faculty Publications

Neolepisorus dengii, N. dengii f. hastatus and N. emeiensis f. dissectus should be considered synonyms of N. emeiensis. This treatment is justified on the basis of complete intergradation of the frond forms that supposedly separate these taxa. The intergradation can be found on the same individual. © 2009, Americian Fern Society.

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Extreme Weathering/Erosion During The Miocene Climatic Optimum: Evidence From Sediment Record In The South China Sea, Shiming Wan, Wolfram M. Kürschner, Peter D. Clift, Anchun Li, Tiegang Li Oct 2009

Extreme Weathering/Erosion During The Miocene Climatic Optimum: Evidence From Sediment Record In The South China Sea, Shiming Wan, Wolfram M. Kürschner, Peter D. Clift, Anchun Li, Tiegang Li

Faculty Publications

Investigating the interplay between continental weathering and erosion, climate, and atmospheric CO2 concentrations is significant in understanding the mechanisms that force the Cenozoic global cooling and predicting the future climatic and environmental response to increasing temperature and CO2 levels. The Miocene represents an ideal test case as it encompasses two distinct extreme climate periods, the Miocene Climatic Optimum (MCO) with the warmest time since 35 Ma in Earth's history and the transition to the Late Cenozoic icehouse mode with the establishment of the east Antarctic ice sheet. However the precise role of continental weathering during this period of major climate …

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A Framework For Detecting Glaucomatous Progression In The Optic Nerve Head Of An Eye Using Proper Orthogonal Decomposition, Madhusudhanan Balasubramanian, Stanislav Žabić, Christopher Bowd, Hilary W. Thompson, Peter Wolenski, S. Sitharama Iyengar, Bijaya B. Karki, Linda M. Zangwill Sep 2009

A Framework For Detecting Glaucomatous Progression In The Optic Nerve Head Of An Eye Using Proper Orthogonal Decomposition, Madhusudhanan Balasubramanian, Stanislav Žabić, Christopher Bowd, Hilary W. Thompson, Peter Wolenski, S. Sitharama Iyengar, Bijaya B. Karki, Linda M. Zangwill

Faculty Publications

Glaucoma is the second leading cause of blindness worldwide. Often, the optic nerve head (ONH) glaucomatous damage and ONH changes occur prior to visual field loss and are observable in vivo. Thus, digital image analysis is a promising choice for detecting the onset and/or progression of glaucoma. In this paper, we present a new framework for detecting glaucomatous changes in the ONH of an eye using the method of proper orthogonal decomposition (POD). A baseline topograph subspace was constructed for each eye to describe the structure of the ONH of the eye at a reference/baseline condition using POD. Any glaucomatous …

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Ethylene Dichloride Disruption Of Fertility In Male Mice, Harold J. Daigle, Derek N. Cole, J. Andrew Carlson, William R. Lee, Vincent L. Wilson Sep 2009

Ethylene Dichloride Disruption Of Fertility In Male Mice, Harold J. Daigle, Derek N. Cole, J. Andrew Carlson, William R. Lee, Vincent L. Wilson

Faculty Publications

Ethylene dichloride (EDC) is a high use compound in chemical industry today. Although a potent alkylating agent and carcinogen, EDC has not been associated previously with adverse fertility consequences. Intraperitoneal 5 to 40 mg/kg once a day for five days in C57BL/6 mice rendered males infertile for 6 months and longer. Two of three mice treated with EDC at 5 mg/kg recovered to fertility after a 3 and 5-week sterile period, respectively. Significant testicular pathology was evident within 8 days post treatment with EDC, which progressed to resemble Sertoli Cell-Only syndrome. Although the observed effects on Leydig cell populations were …

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The Continuity Of Protein Structure Space Is An Intrinsic Property Of Proteins, Jeffrey Skolnick, Adrian K. Arakaki, Yup Lee Seung, Michal Brylinski Sep 2009

The Continuity Of Protein Structure Space Is An Intrinsic Property Of Proteins, Jeffrey Skolnick, Adrian K. Arakaki, Yup Lee Seung, Michal Brylinski

Faculty Publications

The classical view of the space of protein structures is that it is populated by a discrete set of protein folds. For proteins up to 200 residues long, by using structural alignments and building upon ideas of the completeness and continuity of structure space, we show that nearly any structure is significantly related to any other using a transitive set of no more than 7 intermediate structurally related proteins. This result holds for all structures in the Protein Data Bank, even when structural relationships between evolutionary related proteins (as detected by threading or functional analyses) are excluded. A similar picture …

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Identification Of An Antisense Transcript To Zim2 In The Primate Lineage, Jennifer M. Huang, Sungryul Yu, Joomyeong Kim Sep 2009

Identification Of An Antisense Transcript To Zim2 In The Primate Lineage, Jennifer M. Huang, Sungryul Yu, Joomyeong Kim

Faculty Publications

In this study, we identified an antisense transcript to ZIM2 (zinc finger imprinted gene 2) in the human, called ZIM2as. Sequence analysis of the 110 kb region spanned by this transcript revealed a cluster of tandemly repeated sequence in the human, orangutan, and chimpanzee as well as a loss of approximately 70 kb from the corresponding region in the rhesus. The homologous region in most mammals contains a cluster of olfactory receptor (OLFR) genes, but this gene cluster has been lost from the primate lineage. Expression analyses confirmed that ZIM2as is expressed in the human brain and testis. Two CpG …

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High Efficiency Lipid-Based Sirna Transfection Of Adipocytes In Suspension, Gail Kilroy, David H. Burk, Z. Elizabeth Floyd Sep 2009

High Efficiency Lipid-Based Sirna Transfection Of Adipocytes In Suspension, Gail Kilroy, David H. Burk, Z. Elizabeth Floyd

Faculty Publications

Background: Fully differentiated adipocytes are considered to be refractory to introduction of siRNA via lipid-based transfection. However, large scale siRNA-based loss-of-function screening of adipocytes using either electroporation or virally-mediated transfection approaches can be prohibitively complex and expensive. Methodology/Principal Findings: We present a method for introducing small interfering RNA (siRNA) into differentiated 3T3-L1 adipocytes and primary human adipocytes using an approach based on forming the siRNA/ cell complex with the adipocytes in suspension rather than as an adherent monolayer, a variation of "reverse transfection". Conclusions/Significance: Transfection of adipocytes with siRNA by this method is economical, highly efficient, has a simple workflow, …

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The Binding Of Analogs Of Porphyrins And Chlorins With Elongated Side Chains To Albumin, Shimshon Ben Dror, Irena Bronshtein, Hana Weitman, Kevin M. Smith, William G. O'Neal, Peter A. Jacobi, Benjamin Ehrenberg Sep 2009

The Binding Of Analogs Of Porphyrins And Chlorins With Elongated Side Chains To Albumin, Shimshon Ben Dror, Irena Bronshtein, Hana Weitman, Kevin M. Smith, William G. O'Neal, Peter A. Jacobi, Benjamin Ehrenberg

Faculty Publications

In previous studies, we demonstrated that elongation of side chains of several sensitizers endowed them with higher affinity for artificial and natural membranes and caused their deeper localization in membranes. In the present study, we employed eight hematoporphyrin and protoporphyrin analogs and four groups containing three chlorin analogs each, all synthesized with variable numbers of methylenes in their alkyl carboxylic chains. We show that these tetrapyrroles' affinity for bovine serum albumin (BSA) and their localization in the binding site are also modulated by chain lengths. The binding constants of the hematoporphyrins and protoporphyrins to BSA increased as the number of …

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Releases, Distribution And Abundance Of Gratiana Boliviana (Coleoptera: Chrysomelidae), A Biological Control Agent Of Tropical Soda Apple (Solanum Viarum, Solanaceae) In Florida, W. A. Overholt, R. Diaz, K. L. Hibbard, A. L. Roda, D. Amalin, A. J. Fox, S. D. Hight, J. C. Medal, P. A. Stansly, B. Carlisle, J. H. Walter, P. J. Hogue, L. A. Gary, L. F. Wiggins, C. L. Kirby, S. C. Crawford Sep 2009

Releases, Distribution And Abundance Of Gratiana Boliviana (Coleoptera: Chrysomelidae), A Biological Control Agent Of Tropical Soda Apple (Solanum Viarum, Solanaceae) In Florida, W. A. Overholt, R. Diaz, K. L. Hibbard, A. L. Roda, D. Amalin, A. J. Fox, S. D. Hight, J. C. Medal, P. A. Stansly, B. Carlisle, J. H. Walter, P. J. Hogue, L. A. Gary, L. F. Wiggins, C. L. Kirby, S. C. Crawford

Faculty Publications

From 2003 to 2008, 176,643 Gratiana boliviana Spaeth (Coleoptera: Chrysomelidae) were released in Florida as part of a biological control program targeting tropical soda apple (TSA) Solanum viarum Dunal (Solanaceae). The spatial distribution of releases was clustered with more beetles released in south/central Florida than further north. A survey conducted in the fall of 2008 found G. bolviana present at >70% of randomly selected locations between 26° and 29° latitude, but no beetles were found at sites further north. The presence of beetles and beetle damage were associated with smaller TSA plants and fewer fruits per plant. The absence of …

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Evaluation Of Rhodamine B As An Orally Delivered Biomarker For Rodents And A Feed-Through Transtadial Biomarker For Phlebotomine Sand Flies (Diptera: Psychodidae), T. M. Mascari, L. D. Foil Sep 2009

Evaluation Of Rhodamine B As An Orally Delivered Biomarker For Rodents And A Feed-Through Transtadial Biomarker For Phlebotomine Sand Flies (Diptera: Psychodidae), T. M. Mascari, L. D. Foil

Faculty Publications

The purpose of this study was to evaluate the use of rhodamine B as an orally delivered biomarker for rodents and a feed-through transtadial biomarker for phlebotomine sand flies (Diptera: Psychodidae ). Rhodamine B-treated hamsters were visibly marked for up to 8 wk, and their feces were fluorescent when examined under a fluorescence microscope. The development and survival of sand fly larvae fed feces of rhodamine B-treated hamsters were not significantly different from control sand flies. Adult male and female sand flies, that had been fed as larvae the feces of rhodamine B-treated hamsters, were fluorescent when examined using fluorescent …

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Loss Of Halophytism By Interference With Sos1 Expression, Dong Ha Oh, Eduardo Leidi, Quan Zhang, Sung Min Hwang, Youzhi Li, Francisco J. Quintero, Xingyu Jiang, Matilde Paino D'Urzo, Sang Yeol Lee, Yanxiu Zhao, Jeong Dong Bahk, Ray A. Bressan, Dae Jin Yun, José M. Pardo, Hans J. Bohnert Sep 2009

Loss Of Halophytism By Interference With Sos1 Expression, Dong Ha Oh, Eduardo Leidi, Quan Zhang, Sung Min Hwang, Youzhi Li, Francisco J. Quintero, Xingyu Jiang, Matilde Paino D'Urzo, Sang Yeol Lee, Yanxiu Zhao, Jeong Dong Bahk, Ray A. Bressan, Dae Jin Yun, José M. Pardo, Hans J. Bohnert

Faculty Publications

The contribution of SOS1 (for Salt Overly Sensitive 1), encoding a sodium/proton antiporter, to plant salinity tolerance was analyzed in wild-type and RNA interference (RNAi) lines of the halophytic Arabidopsis (Arabidopsis thaliana)-relative Thellungiella salsuginea. Under all conditions, SOS1 mRNA abundance was higher in Thellungiella than in Arabidopsis. Ectopic expression of the Thellungiella homolog ThSOS1 suppressed the salt-sensitive phenotype of a Saccharomyces cerevisiae strain lacking sodium ion (Na+) efflux transporters and increased salt tolerance of wild-type Arabidopsis. thsos1-RNAi lines of Thellungiella were highly salt sensitive. A representative line, thsos1-4, showed faster Na+ accumulation, more severe water loss in shoots under salt …

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Mobile Elements Create Structural Variation: Analysis Of A Complete Human Genome, Jinchuan Xing, Yuhua Zhang, Kyudong Han, Abdel Halim Salem, Shurjo K. Sen, Chad D. Huff, Qiong Zhou, Ewen F. Kirkness, Samuel Levy, Mark A. Batzer, Lynn B. Jorde Sep 2009

Mobile Elements Create Structural Variation: Analysis Of A Complete Human Genome, Jinchuan Xing, Yuhua Zhang, Kyudong Han, Abdel Halim Salem, Shurjo K. Sen, Chad D. Huff, Qiong Zhou, Ewen F. Kirkness, Samuel Levy, Mark A. Batzer, Lynn B. Jorde

Faculty Publications

Structural variants (SVs) are common in the human genome. Because approximately half of the human genome consists of repetitive, transposable DNA sequences, it is plausible that these elements play an important role in generating SVs in humans. Sequencing of the diploid genome of one individual human (HuRef) affords us the opportunity to assess, for the first time, the impact of mobile elements on SVs in an individual in a thorough and unbiased fashion. In this study, we systematically evaluated more than 8000 SVs to identify mobile element-associated SVs as small as 100 bp and specific to the HuRef genome. Combining …

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R A Fisher, Design Theory, And The Indian Connection, A. R.P. Rau Sep 2009

R A Fisher, Design Theory, And The Indian Connection, A. R.P. Rau

Faculty Publications

Design Theory, a branch of mathematics, was born out of the experimental statistics research of the population geneticist R A Fisher and of Indian mathematical statisticians in the 1930s. The field combines elements of combinatorics, finite projective geometries, Latin squares, and a variety of further mathematical structures, brought together in surprising ways. This essay will present these structures and ideas as well as how the field came together, in itself an interesting story. © Indian Academy of Sciences.

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Effects Of Temperature And Uv Light On Degradation Of Α-Tocopherol In Free And Dissolved Form, Cristina M. Sabliov, C. Fronczek, C. E. Astete, M. Khachaturyan, L. Khachatryan, C. Leonardi Sep 2009

Effects Of Temperature And Uv Light On Degradation Of Α-Tocopherol In Free And Dissolved Form, Cristina M. Sabliov, C. Fronczek, C. E. Astete, M. Khachaturyan, L. Khachatryan, C. Leonardi

Faculty Publications

The effects of heat and UV exposure on the degradation of free α-tocopherol (oil form), α-tocopherol dissolved in methanol, and α-tocopherol dissolved in hexane were measured. Results showed that degradation of free α-tocopherol due to heat followed first order kinetics, with the samples held at 180 °C showing the greatest degradation rate. Free α-tocopherol degraded faster at high temperatures than dissolved α-tocopherol. In contrast, free α-tocopherol did not degrade when exposed to UV light for as long as 6 h, but the hexane and methanol samples degraded significantly as a matter of time. The α-tocopherol dissolved in hexane and methanol …

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Yy1 Is Autoregulated Through Its Own Dna-Binding Sites, Jeong D. Kim, Sungryul Yu, Joomyeong Kim Aug 2009

Yy1 Is Autoregulated Through Its Own Dna-Binding Sites, Jeong D. Kim, Sungryul Yu, Joomyeong Kim

Faculty Publications

Background: The transcription factor Yin Yang 1 (YY1) is a ubiquitously expressed, multifunctional protein that controls a large number of genes and biological processes in vertebrates. As a general transcription factor, the proper levels of YY1 protein need to be maintained for the normal function of cells and organisms. However, the mechanism for the YY1 homeostasis is currently unknown. Results: The current study reports that the YY1 gene locus of all vertebrates contains a cluster of its own DNA-binding sites within the 1st intron. The intact structure of these DNA-binding sites is absolutely necessary for transcriptional activity of the YY1 …

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