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Aconitic Acid, Glycerol And Cinnamic Acid Polyester Characterization For Bone Tissue Engineering, Katie Joanna Hogan Jan 2016

Aconitic Acid, Glycerol And Cinnamic Acid Polyester Characterization For Bone Tissue Engineering, Katie Joanna Hogan

Honors Capstones

No abstract provided.

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Everglades Mangrove Forest Response To Large-Scale Disturbance: Long-Term Assessment Of Structural And Functional Properties, Tess Marie Danielson Jan 2016

Everglades Mangrove Forest Response To Large-Scale Disturbance: Long-Term Assessment Of Structural And Functional Properties, Tess Marie Danielson

LSU Master's Theses

The impact (i.e., defoliation and mortality) associated with large-scale natural disturbance events is capable of significantly altering ecosystem structure (e.g., biomass) and function (e.g., productivity) in mangrove wetlands. These pulsing events provide an opportunity to assess ecosystem resilience (i.e., recovery to pre-disturbance conditions) by evaluating the immediate and long-term ecosystem response trajectory (i.e., disturbance magnitude, recovery time). I hypothesized that 1) there are specific properties of disturbance that can be characterized and fit to a general forest disturbance model, and that 2) a mangrove forest recovery trajectory (i.e. recovery duration and rate) is dependent on the disturbance magnitude and initial …

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Silicon Fertilization In Rice:Establishment Of Critical Silicon Level And Its Impact On Availability Of Nutrients In Soils Of Louisiana, Wooiklee Sundayboy Paye Jan 2016

Silicon Fertilization In Rice:Establishment Of Critical Silicon Level And Its Impact On Availability Of Nutrients In Soils Of Louisiana, Wooiklee Sundayboy Paye

LSU Master's Theses

While silicon (Si) fertilization is widely practiced in paddy rice production, the establishment of critical soil Si levels has remained understudied. This study was undertaken to: 1) determine the critical soil Si level for rice production in Louisiana using different extraction procedures, and 2) document the relationship between plant-available Si and select essential plant nutrients in soil and their uptake by rice. Field trials were established at 12 sites across Louisiana from 2013 to 2015. Si was applied as silicate slag (SiO3, 14% Si) at the rates of 0, 1, 2, 4, 6, and 8 Mg ha-1. Agricultural lime was …

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Geographic Distribution And Severity Of Strobilurin Fungicide Resistance Among Rhizoctonia Solani On Rice In Southwestern Louisiana, Allysson Gayle Lunos Jan 2016

Geographic Distribution And Severity Of Strobilurin Fungicide Resistance Among Rhizoctonia Solani On Rice In Southwestern Louisiana, Allysson Gayle Lunos

LSU Master's Theses

Strobilurin fungicide resistance threatens rice production in southwestern Louisiana. Strobilurins are the often relied upon method of managing sheath blight development due to the lack of strong genetic resistance to this disease in the currently available rice germplasm. However, isolates of Rhizoctonia solani AG 1-IA causing sheath blight were reported resistant to azoxystrobin near Mowata, LA in 2011. To determine the geographic distribution and severity of azoxystrobin resistance, 40 commercial rice fields in southwestern Louisiana were sampled for isolates of R. solani. Sensitivity to pyraclostrobin and trifloxystrobin was also assessed to investigate cross-resistance. A genetic test was used to …

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Vitamin E Conjugates, And Their Uses As Antioxidants And Prodrug Delivery Vehicles, Cristina M. Sabliov, Carlos E. Astete, David Spivak Dec 2015

Vitamin E Conjugates, And Their Uses As Antioxidants And Prodrug Delivery Vehicles, Cristina M. Sabliov, Carlos E. Astete, David Spivak

College of Engineering Patents

Tocopherol conjugates are disclosed that are useful as antioxidants or as pro-drug delivery vehicles. The conjugates are amphiphilic, and tend to localize at the interface between water domains and lipid domains. A prototype embodiment disclosed is an alpha tocopherol-linker-carnosine conjugate that has been designated VECAR.

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Using An External Exposome Framework To Examine Pregnancy-Related Morbidities And Mortalities: Implications For Health Disparities Research, Tonny J. Oyana, Patricia Matthews-Juarez, Stephania A. Cormier, Xiaoran Xu, Paul D. Juarez Dec 2015

Using An External Exposome Framework To Examine Pregnancy-Related Morbidities And Mortalities: Implications For Health Disparities Research, Tonny J. Oyana, Patricia Matthews-Juarez, Stephania A. Cormier, Xiaoran Xu, Paul D. Juarez

Faculty Publications

© 2015 by the authors; licensee MDPI, Basel, Switzerland. Objective: We have conducted a study to assess the role of environment on the burden of maternal morbidities and mortalities among women using an external exposome approach for the purpose of developing targeted public health interventions to decrease disparities. Methods: We identified counties in the 48 contiguous USA where observed low birthweight (LBW) rates were higher than expected during a five-year study period. The identification was conducted using a retrospective space-time analysis scan for statistically significant clusters with high or low rates by a Discrete Poisson Model. Results: We observed statistically …

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Evaluation Of Colorimetric Assays For Analyzing Reductively Methylated Proteins: Biases And Mechanistic Insights, Pamlea N. Brady, Megan A. Macnaughtan Dec 2015

Evaluation Of Colorimetric Assays For Analyzing Reductively Methylated Proteins: Biases And Mechanistic Insights, Pamlea N. Brady, Megan A. Macnaughtan

Faculty Publications

© 2015 Elsevier Inc. Colorimetric protein assays, such as the Coomassie blue G-250 dye-binding (Bradford) and bicinchoninic acid (BCA) assays, are commonly used to quantify protein concentration. The accuracy of these assays depends on the amino acid composition. Because of the extensive use of reductive methylation in the study of proteins and the importance of biological methylation, it is necessary to evaluate the impact of lysyl methylation on the Bradford and BCA assays. Unmodified and reductively methylated proteins were analyzed using the absorbance at 280 nm to standardize the concentrations. Using model compounds, we demonstrate that the dimethylation of lysyl …

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Ocean Acidification As One Of Multiple Stressors: Growth Response Of Thalassiosira Weissflogii (Diatom) Under Temperature And Light Stress, Uta Passow, Edward A. Laws Dec 2015

Ocean Acidification As One Of Multiple Stressors: Growth Response Of Thalassiosira Weissflogii (Diatom) Under Temperature And Light Stress, Uta Passow, Edward A. Laws

Faculty Publications

Future shifts in phytoplankton composition and productivity are anticipated given that continuing changes are expected in environmental conditions such as temperature, the partial pressure of CO2 (pCO2) and light climate, all of which regulate phytoplankton communities and their physiology through bottom-up control. Culture experiments revealed that future (elevated) pCO2 had no effect on Thalassiosira weissflogii in the absence of environmental stressors, whereas growth rates drastically decreased under future pCO2 when cells were grown under light and temperature stress. Reduction in growth rates and a smaller decline in cellular photosynthesis under high pCO2 were associated with 2- to 3-fold increases in …

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The Invasive Coral Oculina Patagonica Has Not Been Recently Introduced To The Mediterranean From The Western Atlantic Speciation And Evolutionary Genetics, Karine Posbic Leydet, Michael E. Hellberg Dec 2015

The Invasive Coral Oculina Patagonica Has Not Been Recently Introduced To The Mediterranean From The Western Atlantic Speciation And Evolutionary Genetics, Karine Posbic Leydet, Michael E. Hellberg

Faculty Publications

© 2015 Leydet and Hellberg; licensee BioMed Central. Background: Effective policies, management, and scientific research programs depend on the correct identification of invasive species as being either native or introduced. However, many species continue to be misidentified. Oculina patagonica, first recorded in the Mediterranean Sea in 1966, is believed to have been introduced in anthropogenic times and expanding in a west to east direction. However, its present identification and status as a recently introduced species remain to be explored. In this study, we used multi-locus genetic data to test whether O. patagonica in the Mediterranean has been recently introduced from …

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Detection Of Nucleic Acid Sequence Differences Using The Ligase Detection Reaction With Addressable Arrays, Francis Barany, George Barany, Robert P. Hammer, Maria Kempe, Herman Blok, Monib Zirvi Dec 2015

Detection Of Nucleic Acid Sequence Differences Using The Ligase Detection Reaction With Addressable Arrays, Francis Barany, George Barany, Robert P. Hammer, Maria Kempe, Herman Blok, Monib Zirvi

College of Science Patents

The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide …

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Mrna Levels Of Imprinted Genes In Bovine In Vivo Oocytes, Embryos And Cross Species Comparisons With Humans, Mice And Pigs, Zongliang Jiang, Hong Dong, Xinbao Zheng, Sadie L. Marjani, David M. Donovan, Jingbo Chen, Xiuchun Cindy Tian Dec 2015

Mrna Levels Of Imprinted Genes In Bovine In Vivo Oocytes, Embryos And Cross Species Comparisons With Humans, Mice And Pigs, Zongliang Jiang, Hong Dong, Xinbao Zheng, Sadie L. Marjani, David M. Donovan, Jingbo Chen, Xiuchun Cindy Tian

Faculty Publications

Twenty-six imprinted genes were quantified in bovine in vivo produced oocytes and embryos using RNA-seq. Eighteen were detectable and their transcriptional patterns were: largely decreased (MEST and PLAGL1); first decreased and then increased (CDKN1C and IGF2R); peaked at a specific stage (PHLDA2, SGCE, PEG10, PEG3, GNAS, MEG3, DGAT1, ASCL2, NNAT, and NAP1L5); or constantly low (DIRAS3, IGF2, H19 and RTL1). These patterns reflect mRNAs that are primarily degraded, important at a specific stage, or only required at low quantities. The mRNAs for several genes were surprisingly abundant. For instance, transcripts for the maternally imprinted MEST and PLAGL1, were high in …

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Epigenetic Instability At Imprinting Control Regions In A KrasG12d-Induced T-Cell Neoplasm, Corey L. Bretz, Ingeborg M. Langohr, Suman Lee, Joomyeong Kim Dec 2015

Epigenetic Instability At Imprinting Control Regions In A KrasG12d-Induced T-Cell Neoplasm, Corey L. Bretz, Ingeborg M. Langohr, Suman Lee, Joomyeong Kim

Faculty Publications

© 2015 Taylor & Francis Group, LLC. Although aberrant DNA methylation within imprinted domains has been reported in a variety of neoplastic diseases, it remains largely uncharacterized in the context of carcinogenesis. In this study, we induced T-cell lymphoma in mice by employing a breeding scheme involving mouse strains, LSL-KrasG12D and MMTV-Cre. We then systematically surveyed imprinted domains for DNA methylation changes during tumor progression using combined bisulfite restriction analysis and NGS-based bisulfite sequencing. We detected hyper- or hypo-methylation at the imprinting control regions (ICRs) of the Dlk1, Peg10, Peg3, Grb10, and Gnas domains. These DNA methylation changes at ICRs …

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Dna-Binding Motif Of The Imprinted Transcription Factor Peg3, Suman Lee, An Ye, Joomyeong Kim Dec 2015

Dna-Binding Motif Of The Imprinted Transcription Factor Peg3, Suman Lee, An Ye, Joomyeong Kim

Faculty Publications

© 2015 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Peg3 is an imprinted gene that is predicted to encode a DNA-binding zinc finger protein. This was previously demonstrated through Chromatin ImmunoPrecipitation-based Sequencing experiments. In the current study, we reanalyzed the previous ChIP-Seq results and further characterized the DNA-binding motif of PEG3. According to the results, PEG3 binds to the promoters and enhancers of a subset of genes that are closely …

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Faculty Senate Newsletter, Holiday 2015, Louisiana State University And Agricultural & Mechanical College Dec 2015

Faculty Senate Newsletter, Holiday 2015, Louisiana State University And Agricultural & Mechanical College

LSU Faculty Senate Publications

Message from President: At the end of the LSU-Texas A&M game, television captured Les Miles standing in the embrace of a burly player, insistently singing the LSU alma mater. Any comedy that might have inhered in such an effort at roughhouse opera deteriorated under the impression that the performance was sending a bullying message to LSU officials about the alleged high level of public support for a coach rumored to be fighting for his job. By exploiting a musical component from the sacraments of the academic community for exclusively personal ends, the beleaguered coach helped everyone to understand what has …

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Solving A Class Of Linear Skorokhod Stochastic Differential Equations, Karl-Heinz Fichtner, Steffen Klaere, Volkmar Liebscher Dec 2015

Solving A Class Of Linear Skorokhod Stochastic Differential Equations, Karl-Heinz Fichtner, Steffen Klaere, Volkmar Liebscher

Communications on Stochastic Analysis

No abstract provided.

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Multitrophic Enemy Escape Of Invasive Phragmites Australis And Its Introduced Herbivores In North America, Warwick J. Allen, Randee E. Young, Ganesh P. Bhattarai, Jordan R. Croy, Adam M. Lambert, Laura A. Meyerson, James T. Cronin Dec 2015

Multitrophic Enemy Escape Of Invasive Phragmites Australis And Its Introduced Herbivores In North America, Warwick J. Allen, Randee E. Young, Ganesh P. Bhattarai, Jordan R. Croy, Adam M. Lambert, Laura A. Meyerson, James T. Cronin

Faculty Publications

© 2015, Springer International Publishing Switzerland. One explanation for why invasive species are successful is that they escape natural enemies from their native range or experience lower attack from natural enemies in the introduced range relative to native species (i.e., the enemy-release hypothesis). However, little is known about how invasive plants interact with co-introduced herbivores or natural enemies of the introduced herbivores. We focus on Phragmites australis, a wetland grass native to Europe (EU) and North America (NA). Within the past 100–150 years, invasive European genotypes of P. australis and several species of specialist Lipara gall flies have spread within …

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Tissue-Specific Contributions Of Paternally Expressed Gene 3 In Lactation And Maternal Care Of Mus Musculus, Wesley D. Frey, Joomyeong Kim Dec 2015

Tissue-Specific Contributions Of Paternally Expressed Gene 3 In Lactation And Maternal Care Of Mus Musculus, Wesley D. Frey, Joomyeong Kim

Faculty Publications

© 2015 Frey, Kim.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in anymedium, provided the original author and source are credited. Paternally Expressed Gene 3 (Peg3) is an imprinted gene that controls milk letdown and maternal-caring behaviors. In this study, a conditional knockout allele has been developed in Mus musculus to further characterize these known functions of Peg3 in a tissue-specific manner. The mutant line was first crossed with a germline Cre. The progeny of this cross displayed growth retardation phenotypes. This is consistent with …

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The Seesaw Effect Of Winter Temperature Change On The Recruitment Of Cotton Bollworms Helicoverpa Armigera Through Mismatched Phenology, Gadi V.P. Reddy, Peijian Shi, Cang Hui, Xiaofei Cheng, Fang Ouyang, Feng Ge Dec 2015

The Seesaw Effect Of Winter Temperature Change On The Recruitment Of Cotton Bollworms Helicoverpa Armigera Through Mismatched Phenology, Gadi V.P. Reddy, Peijian Shi, Cang Hui, Xiaofei Cheng, Fang Ouyang, Feng Ge

Faculty Publications

Knowing how climate change affects the population dynamics of insect pests is critical for the future of integrated pest management. Rising winter temperatures from global warming can drive increases in outbreaks of some agricultural pests. In contrast, here we propose an alternative hypothesis that both extremely cold and warm winters can mismatch the timing between the eclosion of overwintering pests and the flowering of key host plants. As host plants normally need higher effective cumulative temperatures for flowering than insects need for eclosion, changes in flowering time will be less dramatic than changes in eclosion time, leading to a mismatch …

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Mathematics Library News 14, Aaron Lercher Dec 2015

Mathematics Library News 14, Aaron Lercher

Faculty Publications

No abstract provided.

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Failure Of A Heterologous Recombinant Sca5/Ompb Protein-Based Vaccine To Elicit Effective Protective Immunity Against Rickettsia Rickettsii Infections In C3h/Hen Mice, Sean P. Riley, Marissa M. Cardwell, Yvonne G. Chan, Ludovic Pruneau, Fabio Del Piero, Juan J. Martinez Dec 2015

Failure Of A Heterologous Recombinant Sca5/Ompb Protein-Based Vaccine To Elicit Effective Protective Immunity Against Rickettsia Rickettsii Infections In C3h/Hen Mice, Sean P. Riley, Marissa M. Cardwell, Yvonne G. Chan, Ludovic Pruneau, Fabio Del Piero, Juan J. Martinez

Faculty Publications

Spotted fever group (SFG) rickettsial species are obligate intracellular tick-borne pathogens that are responsible for important human diseases. Previous reports have demonstrated the feasibility of using recombinant surface cell antigen Sca5/OmpB to elicit protective immunity against homologous challenges using murine models of Mediterranean spotted fever and Rocky Mountain spotted fever. In addition, the feasibility of generating cross-protective immunity against related rickettsial species has also been established, but the molecular basis for these phenomena was not explored. Here, we demonstrate that vaccination of C3H/HeN mice with a recombinant OmpB domain derived from Rickettsia conorii induced high titer humoral immune responses that …

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Island Vs. Countryside Biogeography: An Examination Of How Amazonian Birds Respond To Forest Clearing And Fragmentation, Jared D. Wolfe, Philip C. Stouffer, Karl Mokross, Luke L. Powell, Marina M. Anciaes Dec 2015

Island Vs. Countryside Biogeography: An Examination Of How Amazonian Birds Respond To Forest Clearing And Fragmentation, Jared D. Wolfe, Philip C. Stouffer, Karl Mokross, Luke L. Powell, Marina M. Anciaes

Faculty Publications

Avian diversity in fragmented Amazonian landscapes depends on a balance between extinction and colonization in cleared and disturbed areas. Regenerating forest facilitates bird dispersal within degraded Amazonian landscapes and may tip the balance in favor of persistence in habitat patches. Determining the response of Amazonian birds to fragmentation may be hindered because many species use adjacent second growth matrices thereby limiting the applicability of island biogeography to predict species loss; alternatively, a countryside biogeographic framework to evaluate the value of regenerating forest may be more appropriate. Here, we used point-count and capture data to compare Amazonian bird communities among continuous …

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Environmentally Persistent Free Radical-Containing Particulate Matter Competitively Inhibits Metabolism By Cytochrome P450 1a2, James R. Reed, Albert Leo N. Dela Cruz, Slawo M. Lomnicki, Wayne L. Backes Dec 2015

Environmentally Persistent Free Radical-Containing Particulate Matter Competitively Inhibits Metabolism By Cytochrome P450 1a2, James R. Reed, Albert Leo N. Dela Cruz, Slawo M. Lomnicki, Wayne L. Backes

Faculty Publications

Combustion processes generate different types of particulate matter (PM) that can have deleterious effects on the pulmonary and cardiovascular systems. Environmentally persistent free radicals (EPFRs) represent a type of particulate matter that is generated after combustion of environmental wastes in the presence of redox-active metals and aromatic hydrocarbons. Cytochromes P450 (P450/CYP) are membrane-bound enzymes that are essential for the phase I metabolism of most lipophilic xenobiotics. The EPFR formed by chemisorption of 2-monochlorophenol to silica containing 5% copper oxide (MCP230) has been shown to generally inhibit the activities of different forms of P450s without affecting those of cytochrome P450 reductase …

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Colorimetric And Fluorometric Determination Of Homocysteine And Cysteine, Robert M. Strongin, Weihua Wang, Oleksandr Rusin, Nadia N. St. Luce, Jorge O. Escobedo Dec 2015

Colorimetric And Fluorometric Determination Of Homocysteine And Cysteine, Robert M. Strongin, Weihua Wang, Oleksandr Rusin, Nadia N. St. Luce, Jorge O. Escobedo

College of Science Patents

Colorimetric and fluorometric methods are disclosed for the rapid, accurate, selective, and inexpensive detection of homocysteine, or of homocysteine and cysteine, or of cysteine. The methods may be employed with materials that are readily available commercially. The novel methods are selective for homocysteine, for cysteine, or for total homocysteine and cysteine, and do not cross-react substantially with chemically-related species such as glutathione. The homocysteine-selective method does not have substantial cross-reactivity to the very closely related species cysteine. The cysteine-selective method does not have substantial cross-reactivity to the very closely related species homocysteine. The methods may be used, for example, in …

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Phylogeography Of The Military Macaw (Ara Militaris) And The Great Green Macaw (A. Ambiguus) Based On Mtdna Sequence Data, Jessica R. Eberhard, Eduardo E. Iñigo-Elias, Ernesto Enkerlin-Hoeflich, E. Paùl Cun Dec 2015

Phylogeography Of The Military Macaw (Ara Militaris) And The Great Green Macaw (A. Ambiguus) Based On Mtdna Sequence Data, Jessica R. Eberhard, Eduardo E. Iñigo-Elias, Ernesto Enkerlin-Hoeflich, E. Paùl Cun

Faculty Publications

© 2015 The Wilson Ornithological Society. The Military Macaw (Ara militaris) and the Great Green Macaw (A. ambiguus) are species whose close relationship is reflected in their morphological similarity as well as their geographic ranges. Military Macaws have a disjunct distribution, found in Mexico as well as several areas in South America, while Great Green Macaws have two or more disjunct populations from Honduras to eastern Ecuador. We used mitochondrial sequence data to examine the phylogenetic relationships between these two species, and also among representative samples across their ranges. Our data clearly support recognition of the two species as being …

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Identification Of Morphemes And The Effect Of Memory Load On Second Language Learning, James A. Hebert Dec 2015

Identification Of Morphemes And The Effect Of Memory Load On Second Language Learning, James A. Hebert

Honors Capstones

No abstract provided.

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Using Foraminifera Shell Geochemistry To Test Proxies For Paleoenvironmental Reconstruction, Catherine Hudson Dec 2015

Using Foraminifera Shell Geochemistry To Test Proxies For Paleoenvironmental Reconstruction, Catherine Hudson

Honors Capstones

No abstract provided.

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The Effect Of Dietary Salt Intake On The Subcellular Distribution Of Epithelial Na+ Channels In Vasopressin And Oxytocin Synthesizing Neurons In The Rat Hypothalamus, Richard Frank Guidry Dec 2015

The Effect Of Dietary Salt Intake On The Subcellular Distribution Of Epithelial Na+ Channels In Vasopressin And Oxytocin Synthesizing Neurons In The Rat Hypothalamus, Richard Frank Guidry

Honors Capstones

No abstract provided.

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Predicted Binding Site Information Improves Model Ranking In Protein Docking Using Experimental And Computer-Generated Target Structures, Surabhi Maheshwari, Michal Brylinski Nov 2015

Predicted Binding Site Information Improves Model Ranking In Protein Docking Using Experimental And Computer-Generated Target Structures, Surabhi Maheshwari, Michal Brylinski

Faculty Publications

© 2015 Maheshwari and Brylinski. Background: Protein-protein interactions (PPIs) mediate the vast majority of biological processes, therefore, significant efforts have been directed to investigate PPIs to fully comprehend cellular functions. Predicting complex structures is critical to reveal molecular mechanisms by which proteins operate. Despite recent advances in the development of new methods to model macromolecular assemblies, most current methodologies are designed to work with experimentally determined protein structures. However, because only computer-generated models are available for a large number of proteins in a given genome, computational tools should tolerate structural inaccuracies in order to perform the genome-wide modeling of PPIs. …

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Novel Mode Of Defective Neural Tube Closure In The Non-Obese Diabetic (Nod) Mouse Strain, J. Michael Salbaum, Claudia Kruger, Jacalyn Macgowan, Nils J. Herion, David Burk, Claudia Kappen Nov 2015

Novel Mode Of Defective Neural Tube Closure In The Non-Obese Diabetic (Nod) Mouse Strain, J. Michael Salbaum, Claudia Kruger, Jacalyn Macgowan, Nils J. Herion, David Burk, Claudia Kappen

Faculty Publications

Failure to close the neural tube results in birth defects, with severity ranging from spina bifida to lethal anencephaly. Few genetic risk factors for neural tube defects are known in humans, highlighting the critical role of environmental risk factors, such as maternal diabetes. Yet, it is not well understood how altered maternal metabolism interferes with embryonic development, and with neurulation in particular. We present evidence from two independent mouse models of diabetic pregnancy that identifies impaired migration of nascent mesodermal cells in the primitive streak as the morphogenetic basis underlying the pathogenesis of neural tube defects. We conclude that perturbed …

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Multipart Chaperone-Effector Recognition In The Type Iii Secretion System Of Chlamydia Trachomatis, Li Shen, Megan A. Macnaughtan, Kyla M. Frohlich, Yanguang Cong, Octavia Y. Goodwin, Chau Wen Chou, Louis Lecour, Kristen Krup, Miao Luo, David K. Worthylake Nov 2015

Multipart Chaperone-Effector Recognition In The Type Iii Secretion System Of Chlamydia Trachomatis, Li Shen, Megan A. Macnaughtan, Kyla M. Frohlich, Yanguang Cong, Octavia Y. Goodwin, Chau Wen Chou, Louis Lecour, Kristen Krup, Miao Luo, David K. Worthylake

Faculty Publications

© 2015 by The American Society for Biochemistry and Molecular Biology, Inc. Secretion of effector proteins into the eukaryotic host cell is required for Chlamydia trachomatis virulence. In the infection process, Scc1 and Scc4, two chaperones of the type III secretion (T3S) system, facilitate secretion of the important effector and plug protein, CopN, but little is known about the details of this event. Here we use biochemistry, mass spectrometry, nuclear magnetic resonance spectroscopy, and genetic analyses to characterize this trimolecular event. We find that Scc4 complexes with Scc1 and CopN in situ at the late developmental cycle of C. trachomatis. …

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