Digital Commons Network^™

Corrigendum: Diatom Transcriptional And Physiological Responses To Changes In Iron Bioavailability Across Ocean Provinces [Front. Mar. Sci., 4, (2017), (360)] Doi: 10.3389/Fmars.2017.00360, Natalie R. Cohen, Kelsey A. Ellis, Robert H. Lampe, Heather Mcnair, Benjamin S. Twining, Maria T. Maldonado, Mark A. Brzezinski, Fedor I. Kuzminov, Kimberlee Thamatrakoln, Claire P. Till, Kenneth W. Bruland, William G. Sunda, Sibel Bargu, Adrian Marchetti

Faculty Publications

In the original Acknowledgments of this article, we neglected to thank Carolyn Duckham for her help collecting and processing samples for DIC uptake rates at stations C1-C3. The authors regret this oversight. This error does not change the scientific conclusions of the article in any way. The original article has been updated.

A Computational Reconstruction Of Papio Phylogeny Using Alu Insertion Polymorphisms, Vallmer E. Jordan, Jerilyn A. Walker, Thomas O. Beckstrom, Cody J. Steely, Cullen L. Mcdaniel, Corey P. St Romain, Kim C. Worley, Jane Phillips-Conroy, Clifford J. Jolly, Jeffrey Rogers, Miriam K. Konkel, Mark A. Batzer, Jeffrey Rogers, R. Alan Harris, Muthuswamy Raveendran, Yue Liu, Shwetha Murali, Tauras P. Vilgalys, Jerilyn A. Walker, Miriam K. Konkel

Faculty Publications

© 2018 The Author(s). Background: Since the completion of the human genome project, the diversity of genome sequencing data produced for non-human primates has increased exponentially. Papio baboons are well-established biological models for studying human biology and evolution. Despite substantial interest in the evolution of Papio, the systematics of these species has been widely debated, and the evolutionary history of Papio diversity is not fully understood. Alu elements are primate-specific transposable elements with a well-documented mutation/insertion mechanism and the capacity for resolving controversial phylogenetic relationships. In this study, we conducted a whole genome analysis of Alu insertion polymorphisms unique to …

Characterization Of The Interaction Between R. Conorii And Human Host Vitronectin In Rickettsial Pathogenesis, Abigail Inez Fish

LSU Doctoral Dissertations

Spotted Fever Group Rickettsia are inoculated into the mammalian host during hematophagous arthropod feeding. Once in the bloodstream and during dissemination, the survival of these pathogens is dependent upon their ability to evade innate host defenses until a proper cellular target is reached. The establishment of a successful infection also relies on the ability of the bacteria to attach and invade target cells, as failure to do so results in destruction of the bacterium. Rickettsia conorii expresses an outer membrane protein, Adr1, which binds the multifunctional human glycoprotein, vitronectin, to promote resistance to complement mediated killing. Homologs of Adr1 are …

Workflows For X-Ray And Neutron Interferometry/Tomography As Applied To Additive Manufacturing, Jumao Yuan

LSU Doctoral Dissertations

Grating-based interferometry/tomography is being rapidly developed for non-destructive evaluation of additive manufacturing test articles. An application requiring an efficient workflow is extremely necessary for stress and fatigue testing samples.

At present, scientific workflows play an important role for computational experiments in additive manufacturing 3D printing and interferometry/tomography imaging analysis. A clear workflow template allows scientists to process experiments easier and faster. Work flow library grows, but to find an appropriate workflow for their task is challenging. In our research, there are mainly three portions in the workflow, interferometry analysis, image reconstruction and 3D visualization. Currently, the hierarchy of workflows in …

An Evaluation Of Pathophysiology And Biomechanics Of Selected Lamenesses In The Horse, Cole Barrett Sandow

LSU Master's Theses

INTRODUCTION-Laminitis and synovitis are two common causes of equine lameness. Laminitis is most often associated with hyperinsulinemia and osteoarthritis is most commonly the result of synovitis. The objective is to evaluate biomechanical and pathophysiologic events regarding laminitis and synovitis.

MATERIALS AND METHODS-The first study evaluated biomechanical effects of insulin on lamellar strength. For this experiment, lamellar explants were harvested, incubated in media only (serving as a control) or media with a high concentration of insulin for 8 hours. After incubation, structural integrity was evaluated with a mechanical testing device. Data included load to failure (N), stress to failure (MPa), elongation …

Press To Stop, Garrett Duncan Hazelwood

LSU Master's Theses

In the wake of a performance art piece in which the audience kills one of the performers and maims the other, the surviving artist is put on display in art galleries by his family. The public clamors to see him, protests against his exploitation rock the city, and intrigue surrounds his sudden rise to fame. In the end, a religion is founded in his honor and his best friend must make a terrible choice about whether or not to save him.

Nutrition Misconceptions And Awareness Among College Students In A University Community, Shervia Singleton Taylor

LSU Doctoral Dissertations

Identification of misconceptions of macronutrients (carbohydrate, protein, and fat ) and awareness of ailments linked to micronutrients (potassium, fiber, Vitamin D. and food label use) based on certain demographics are vital to improving and developing long term health benefits, increasing consumption of fruits and vegetables, and decreasing healthcare cost associated with poor dietary lifestyles among non-major general biology college students at a historically black college and university located in the southern part of the United States. Misconception Identification (all groups) was determined using the pretest and posttest Nutrition Knowledge Test via Qualtrics that included two and three tiered question format …

The Effects Of Metal Speciation, Content And Cluster Size In Fly Ash On Pcdd/F Formation, Xia Guan

LSU Doctoral Dissertations

Transition metal oxides present in waste incineration systems have the ability to catalyze the formation of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) through surface mediated condensation of organic dioxin precursors. Current studies have concentrated on the catalytic effects of individual transition metal oxides, while the complex composition of fly ash introduces the possibility of synergistic or antagonistic effects between multiple, catalytically active components. In addition, there is at present hardly a quantitative link between fly ash physical properties/chemical reactivity and PCDD/Fs yield.

We have tested fly ash surrogates containing different ratios of iron (III) oxide and copper (II) oxide …

Functional Plain Yogurt Enhanced With Inulin And Aloe Vera, Janny Melissa Mendoza Mencia

LSU Master's Theses

Yogurt has been recognized as a healthy food. Nutritional value of yogurt can be increased with the addition of aloe vera juice (AVJ) and inulin. People are interested in consuming products without artificial ingredients and with high content of bioactive compounds. The aims of this study were to develop a functional plain yogurt enhanced with inulin and AVJ, to evaluate the physicochemical properties, the enumeration of Streptococcus thermophilus and Lactobacillus bulgaricus, inulin content, and consumer acceptability. Six treatments were evaluated at day 1, 7, 14 and 30 at 4°C. Treatments were prepared using 2% fat milk, ABY culture, 5 …

Whole Tart Cherry Improves Disease Activity Index In Rat Model Of Dextran Sulfate Solution – Induced Ulcerative Colitis By Downregulating The Janus 1 And Janus 3 Kinases And Tnf - Alpha, Johana Alexandra Coronel

LSU Master's Theses

Ulcerative colitis (UC) is a disabling inflammatory bowel disease. UC is characterized by chronic, relapsing inflammation of the colon and rectum. Current treatments such as monoclonal antibodies against TNF-α, IL-6, IL-12/p40, adhesion molecules, Janus kinases (JAK) inhibitors have side effects or lose their effects over time. Alternative approaches with fewer side effects for patients are needed. Montmorency tart cherries (Prunus cerasus) are a good source of anti-inflammatory flavonoids. We hypothesized that regular consumption whole tart cherry (TC) standardized to its major anthocyanin content, namely cyanidin-3-glucosyl-rutinoside, would be effective in reducing inflammation in UC. The aim of this research was to …

Cloning, Purification, And Preliminary Dna-Binding And Unfolding Results For The Dna Polymerase I From The Psychrophile Psychromonas Ingrahamii, John Tod Baker

LSU Master's Theses

Psychromonas ingrahamii is a psychrophilic bacterium that lives in Arctic polar sea ice and grows at a temperature range of -12 to 10º C. This bacterium resides within veins inside the ice where the salinity is high, resulting in a freezing point depression and liquid water. The large fragment of DNA polymerase I from Psychromonas ingrahamii, called Klenpin, has been cloned, expressed, and purified in our laboratory. Although enzyme kinetic studies have been performed on a few psychrophilic enzymes, the thermodynamics of ligand binding and of protein stability have not been well studied for this class of extremophilic proteins. …

Brewed Awakening: Re-Imagining Education In Three Nineteenth-Century New Orleans Coffee Houses, Robyn Rene Andermann

LSU Doctoral Dissertations

In dominant narratives of the history of education in America, the icon of the American educated citizen has traditionally been rooted in Jeffersonian Democracy, eventually taking shape in the Northern, Anglo-Protestant, Common School Movement in which the availability and acceptance of state-supported public education was a key measure of democratic progress. Within the institution of common schools, individuals were taught how to participate in a democratic society.

This dissertation reimagines the dominant narrative by suggesting that the multiethnic and multilingual nature of New Orleans, which some early American leaders had framed as discordant and disorderly, was vital to constructing an …

Formation Control Of Multi-Agent Systems By Using Rigid Graphs, Pengpeng Zhang

LSU Doctoral Dissertations

This research is devoted to the decentralized formation control of multi-agent systems in two- and three-dimensional space. Rigid graph theory is used to describe the interactions between the agents, and Lyapunov theory and input-to-state stability are the main tools for designing and analyzing the stability of the proposed formation controllers. Two formation problems are addressed in this work: formation acquisition, where the goal is for the agents to form and maintain a pre-defined geometric shape; and formation maneuvering, where the agents need to acquire a formation and move cohesively as a virtual rigid body.

In the first problem, we tackle …

Analysis Of Submerged Aquatic Vegetation Resources Across The Northern Gulf Of Mexico: Communities And Biomass, Eva R. Hillmann

LSU Doctoral Dissertations

Submerged aquatic vegetation (SAV) provides many critical ecosystem services, yet we lack basic information on SAV assemblages, biomass and diversity across expansive coasts such as the northern Gulf of Mexico (nGoM). This research investigated SAV along the nGoM from 2013-2015 examining (1) inter-annual variation in SAV assemblages and biomass across salinity zones and gulf coast eco-regions (Texas Mid-Coast, Texas/LA Chenier Plain, Louisiana Delta, MS/AL), (2) intra-annual variation in SAV assemblages and biomass across salinity zones, (3) response of two species, Ruppia maritima and Myriophyllum spicatum, to salinity and light regimes, and (4) estimated organic carbon stock and storage of …

Breaking Conventions: Ohrr, A Transcriptional Regulator From Burkholderia Thailandensis, Anuja Rajiv Pande

LSU Doctoral Dissertations

Organic peroxides (OPs) are highly toxic oxidants. They directly react with cellular macromolecules rendering them inactive. Soil bacteria live in an oxidative environment rich in OPs from plant exudates and other xenobiotic compounds. Bacteria also face a burst of organic peroxides (lipid peroxides) in the macrophages during host invasion. Prokaryotes sense oxidative changes in environment and modulate their gene expression as an adaptive response for survival. MarR (multiple antibiotic resistance regulators) proteins enable bacteria to respond to such stress. Resistance to organic oxidants has been hypothesized to help bacteria survive in presence of disinfectants. This increases nosocomial infection rate.

Bacteria …

Linguistic Political Ecology With The Ngäbe Indigenous People Of Panama, Ginés A. Sánchez Arias

LSU Doctoral Dissertations

Indigenous communities from all corners of the globe live in uncertain times. From the vantage point of their “remote" lands, they undergo some of globalization’s most harmful externalities. Their homes become increasingly harder to maintain as extractive industries, development schemes, clandestine land grabs, and national bureaucracies encroach creating new colonial lands. First by assimilation, and then integration, these processes systematically undermine indigenous culture and autonomy. In place of such destructive coloniality, indigenous societies shelter unique ecological and linguistic knowledge that continues to serve their progress. This research applies lessons learned from studying with Ngäbe communities of western Panama, towards a …

The Relationship Between Maternal Distress And Adjustment Problems In Adolescents With Attention-Deficit/Hyperactivity Disorder: An Examination Of Family Routines And Communication As Moderators, Ryan Nicole Cummins

LSU Master's Theses

Attention-Deficit/Hyperactivity Disorder (ADHD) is a common disorder in youth. The presence of comorbid internalizing and externalizing symptoms is a frequent occurrence in ADHD youth; comorbid symptoms are associated with poor adjustment into adolescence and negative trajectories into adulthood. There are many contributing factors in the development of comorbid symptoms (e.g., parental distress and family environment). Thus, it is important to understand the relationship between contributing factors and the ways in which family consistency helps to manage problem behaviors in adolescents with ADHD. One way to increase consistency is through the use of routines and positive communication. The present study examined …

Effects Of Enhanced Efficiency Nitrogen Fertilizers On Agronomic Parameters And Loss Pathways In The Subtropical Mississippi Delta Region, Scott Michael Pensky

LSU Master's Theses

Minimizing nitrogen (N) loss is critical for improving N use efficiency (NUE) in crop production and reducing its effects on the environment. Management practices such as seasonal application timing of N fertilizers and the addition of enhanced efficiency N fertilizers (EENFs) were investigated for the high N requirement of the two most common cereal crops globally, corn (Zea mays L.) and wheat (Triticum aestivum L.), in the subtropical climate of Louisiana. Field research was established during the 2016 and 2017 seasons at the LSU Agricultural Center’s Dean Lee and Central research stations to examine the effect of different EENFs and …

Relating Diets And Food Availability To Long-Term Population Trends Of Lesser Scaup Wintering On Lake Pontchartrain, Louisiana, Clay Michael Stroud

LSU Master's Theses

Lake Pontchartrain provides wintering habitat for a continentally-significant proportion of lesser scaup (Aythya affinis). Mid-winter aerial surveys indicate dramatic variation in annual scaup abundance (0–1,194,907), though the mechanisms driving fluctuations have not been previously investigated. In response to natural and anthropogenic disturbances, species composition, abundance, and size structure of benthic invertebrate populations in Lake Pontchartrain exhibit drastic variability among years. Given that wintering scaup feed primarily on mollusks, environmental disturbances have the potential to influence scaup dynamics on the estuary by altering the benthic community. To diagnose trophic linkages and variation in scaup abundance, we collected ducks (n=60) …

Evaluation Of Controlled-Release Nitrogen And Potassium Fertilizer In Louisiana Sugarcane Production, Samuel Kwakye

LSU Master's Theses

Sugarcane (Saccharum spp) production is an integral part of Louisiana's economy. Proper fertilizer management, particularly nitrogen (N) and potassium (K), optimizes sugarcane production. An experiment was established in 2015 at the LSU AgCenter Sugar Research Station in St. Gabriel, Louisiana to: (1) assess the effect of N and K fertilizer sources on sugarcane yield and quality parameters, and (2) monitor the changes in inorganic N and K levels in the soil at different sugarcane growth stages. The experiment was arranged in a randomized complete block design on a Sharkey clay soil and complete randomized design on a Commerce …

Effects Of Dimethyl Sulfoxide And Glycerol Based Vitrification Protocols On Zona Pellucida Hardening In Mature Bovine Oocytes, Kaci Denise Rogers

LSU Master's Theses

Zona pellucida hardening is a natural process that occurs after oocyte fertilization to prevent polyspermic fertilization and to protect embryonic development. Pre-fertilization hardening of the zona pellucida however, decreases fertilization rates. Cryoprotectants have also been shown to negatively affect fertilization rates, one possible mechanism of which being through zona hardening. This experiment was conducted to determine the effect of different cryoprotectants on hardening of the zona pellucida using fresh mature bovine oocytes and vitrified mature bovine oocytes. Oocytes were post-slaughter from mixed breed cows. After collection, oocytes were randomly assigned to three cryoprotectant treatment groups: dimethyl sulfoxide (DMSO), glycerol or …

Blue And Black Cloth Targets: Effects Of Size, Shape, And Color On Stable Fly (Diptera: Muscidae) Attraction, Jerome A. Hogsette, Lane D. Foil

Faculty Publications

© 2018 Oxford University Press. All rights reserved. Stable fly management is challenging because of the fly's dispersal behavior and its tendency to remain on the host only while feeding. Optically attractive traps have been used to survey and sometimes reduce adult populations. Insecticide-treated blue and black cloth targets developed for tsetse fly management in Africa were found to be attractive to stable flies in the United States, and various evaluations were conducted in Louisiana and Florida. Tests using untreated targets were designed to answer questions about configuration, size, and color relative to efficacy and stability in high winds. Studies …

Characterization Of Resistance To Cephus Cinctus (Hymenoptera: Cephidae) In Barley Germplasm, Andrea C. Varella, Luther E. Talbert, Buddhi B. Achhami, Nancy K. Blake, Megan L. Hofland, Jamie D. Sherman, Peggy F. Lamb, Gadi V.P. Reddy, David K. Weaver

Faculty Publications

Most barley cultivars have some degree of resistance to the wheat stem sawfly (WSS), Cephus cinctus Norton (Hymenoptera: Cephidae). Damage caused by WSS is currently observed in fields of barley grown in the Northern Great Plains, but the impact of WSS damage among cultivars due to genetic differences within the barley germplasm is not known. Specifically, little is known about the mechanisms underlying WSS resistance in barley. We characterized WSS resistance in a subset of the spring barley CAP (Coordinated Agricultural Project) germplasm panel containing 193 current and historically important breeding lines from six North American breeding programs. Panel lines …

Mechanical And Physical Properties Of Fluorogypsum-Cement-Fly Ash Blends For Artificial Reef And Coastal Protection Structures, Yasser Bigdeli

LSU Doctoral Dissertations

The State of Louisiana faces severe soil erosion along its coastlines. Coastal protection structures most commonly are made of limestone and concrete. However, in Louisiana, these materials are not widely available, which makes it significantly expensive to protect the coastlines and marine life. This study proposes to use locally available materials that have lower cost and lower density than limestone and concrete to reduce the economic burden of protecting and enhancing coastlines.

This study presents an extensive investigation of strength and durability properties of a low-cost concrete-like composite material made of pH-adjusted fluorogypsum (FG), class C fly ash (FA), and …

Changes In Carbon, Nutrient, And Trace Metal Concentrations During The Transition From Freshwater To Marine Environments - A Case Study In Southwest Louisiana, Songjie He

LSU Doctoral Dissertations

Rivers carry terrestrial nutrients and other materials to estuaries, affecting biogeochemical cycles and ecosystems in coastal margins. This dissertation research incorporated five studies to investigate the dynamics of carbon, nutrients, and trace metals in four major coastal rivers in southwest Louisiana – the Sabine, Calcasieu, Mermentau, and Vermilion Rivers. The overarching goal of this research was to broaden the knowledge and understanding of biogeochemical processes in freshwater – saltwater mixing zones. These studies employed decade-long monitoring records of water chemistry, utilized monthly field measurements conducted over a two-year period, and used analysis of over 200 water samples for carbon, nitrogen, …

Exploring Alternative Giant Salvinia (Salvinia Molesta D.S. Mitchell) Management Strategies, Bradley T. Sartain

LSU Doctoral Dissertations

Dense infestations of the free-floating aquatic fern giant salvinia (Salvinia molesta D.S. Mitchell) have been expanding across the southeastern United States for over two decades. Although herbicide programs have provided relief to giant salvinia infested areas, morphology and growth characteristics make management difficult. A series of mesocosm trials were conducted to explore alternative management strategies for giant salvinia. Evaluation of non-registered aquatic herbicides showed that rates of sulfometuron and metsulfuron controlled giant salvinia and provided a 98 to 100% biomass reduction. An outdoor mesocosm trial evaluated potential glyphosate resistance in giant salvinia in select water bodies throughout Louisiana. Differences …

Mobility Of Nano-Particles In Rock Based Micro-Models, Jagannath Upadhyay

LSU Doctoral Dissertations

A confocal micro-particle image velocimetry (C-μPIV) technique along with associated post-processing algorithms is detailed for obtaining three dimensional distributions of nano-particle velocity and concentrations at select locations of the 2.5D (pseudo 3D) Poly(methyl methacrylate) (PMMA) and ceramic micro-model. The designed and fabricated 2.5D micro-model incorporates microchannel networks with 3D wall structures with one at observation wall which resembles fourteen morphological and flow parameters to those of fully 3D actual reservoir rock (Boise Sandstone) at resolutions of 5 and 10 μm in depth and 5 and 25 μm on plane. In addition, an in-situ, non-destructive method for measuring the geometry of …

Impact Of Sorghum Cultivar, Phenology, Nitrogen, And Silicon Fertilization On Melanaphis Sacchari Biology, Luna Lama

LSU Master's Theses

The sugarcane aphid, Melanaphis sacchari (Zehntner) (Hemiptera: Aphididae), is an invasive pest of grain sorghum, Sorghum bicolor (L.) Moench. Since its first outbreak in sorghum in 2013, severe infestations have spread throughout the southern U.S. causing high economic losses. While insecticidal control has mitigated some of the impacts of this pest, a sustainable ecology-based management program is needed to reduce reliance on chemical control. We studied the effects of silicon (rates equivalent to 0 and 3360 kg Si/ha) and nitrogen (rates equivalent to 0, 110, and 220 kg N/ha) on M. sacchari growth and reproduction on resistant (DKS 37-07) and …

Developing Nanopore Electromechanical Sensors With Transverse Electrodes For The Study Of Nanoparticles/Biomolecules, Mohammadsadegh Beheshti

LSU Doctoral Dissertations

This study concerns development of a technology of utilizing metallic nanowires for a sensing element in nanofluidic single molecular (nanoparticle) sensors formed in plastic substrates to detect the translocation of single molecules through the nanochannel. We aimed to develop nanofluidic single molecular sensors in plastic substrates due to their scalability towards high through and low cost manufacturing for point-of-care applications. Despite significant research efforts recently on the technologies and applications of nanowires, using individual nanowires as electric sensing element in nanofluidic bioanalytic devices has not been realized yet. This dissertation work tackles several technical challenges involved in this development, which …

P3: Phylogenetic Posterior Prediction In Revbayes, Sebastian Höhna, Lyndon M. Coghill, Genevieve G. Mount, Robert C. Thomson, Jeremy M. Brown

Faculty Publications

© The Author 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. Tests of absolute model fit are crucial in model-based inference because poorly structured models can lead to biased parameter estimates. In Bayesian inference, posterior predictive simulations can be used to test absolute model fit. However, such tests have not been commonly practiced in phylogenetic inference due to a lack of convenient and flexible software. Here, we describe our newly implemented tests of model fit using posterior predictive testing, based on both data- and inference-based test statistics, in the phylogenetics software RevBayes. …