Digital Commons Network^™

Making Epidermal Bladder Cells Bigger: Developmental-And Salinity-Induced Endopolyploidy In A Model Halophyte, Bronwyn J. Barkla, Timothy Rhodes, Kieu Nga T. Tran, Chathura Wijesinghege, John C. Larkin, Maheshi Dassanayake

Faculty Publications

© 2018 American Society of Plant Biologists. All rights reserved. Endopolyploidy occurs when DNA replication takes place without subsequent mitotic nuclear division, resulting in cell-specific ploidy levels within tissues. In plants, endopolyploidy plays an important role in sustaining growth and development, but only a few studies have demonstrated a role in abiotic stress response. In this study, we investigated the function of ploidy level and nuclear and cell size in leaf expansion throughout development and tracked cell type-specific ploidy in the halophyte Mesembryanthemum crystallinum. In addition to developmental endopolyploidy, we examined the effects of salinity stress on ploidy level. We …

Putative Resistance-Associated Genes Induced In Sugarcane In Response To The Brown Rust Fungus, Puccinia Melanocephala And Their Use In Genetic Diversity Analysis Of Louisiana Sugarcane Clones, Mavir Carolina Avellaneda, Arnold P. Parco, Jeffrey W. Hoy, Niranjan Baisakh

Faculty Publications

Development of resistant cultivars is the main control measure against sugarcane brown rust caused by Puccinia melanocephala. Durability is uncertain, since the pathogen possesses adaptive ability to overcome host plant resistance. A differential gene expression study utilizing suppressive subtraction hybridization was conducted to identify brown rust responsive genes in sugarcane. The expression patterns of 11 out of 217 unigenes of the subtractive library representing biosynthetic pathways, defense-related genes, and signaling genes were analyzed in L 99-233, a cultivar exhibiting quantitative resistance, L 01-299, a resistant cultivar with the major resistance gene Bru1, and two susceptible cultivars, Ho 95-988 and L …

Using Hydrogen Stable Isotope Ratios To Trace The Geographic Origin Of The Population Of Bactrocera Dorsalis (Diptera: Tephritidae) Trapped In Northern China, Zihua Zhao, Zhenglong Lu, Gadi V.P. Reddy, Shuo Zhao, Guanghui Lin, Jianyun Ding, Jiajiao Wu, Zhihong Li

Faculty Publications

The oriental fruit fly, Bactrocera dorsalis Hendel (Diptera: Tephritidae), is a generalist feeder that is known to successfully feed and breed on a variety of fruits and vegetables in the tropical and subtropical zones of Asia. Recently, the northward extension of B. dorsalis has been reported, and this geographic range increase relates to both climate changes and the rapid development of domestic and international trade and travel. However, it has not been possible to determine the origin of this pest, which is trapped in north China. In this study, traps baited with the sex attractant methyl eugenol were used to …

Stability And Robustness Analysis For A Multispecies Chemostat Model With Delays In The Growth Rates And Uncertainties, Frederic Mazenc, Gonzalo Robledo, Michael Malisoff

Faculty Publications

We study a chemostat model with an arbitrary number of competing species, one substrate, and constant dilution rates. We allow delays in the growth rates and additive uncertainties. Using constant inputs of certain species, we derive bounds on the sizes of the delays that ensure asymptotic stability of an equilibrium when the uncertainties are zero, which can allow persistence of multiple species. Under delays and uncertainties, we provide bounds on the delays and on the uncertainties that ensure input-to-state stability with respect to uncertainties.

Changes To Extender, Cryoprotective Medium, And In Vitro Fertilization Improve Zebrafish Sperm Cryopreservation, Jennifer L. Matthews, Joy M. Murphy, Carrie Carmichael, Huiping Yang, Terrence Tiersch, Monte Westerfield, Zoltan M. Varga

Faculty Publications

Sperm cryopreservation is a highly efficient method for preserving genetic resources. It extends the reproductive period of males and significantly reduces costs normally associated with maintenance of live animal colonies. However, previous zebrafish (Danio rerio) cryopreservation methods have produced variable outcomes and low post-thaw fertilization rates. To improve post-thaw fertilization rates after cryopreservation, we developed a new extender and cryoprotective medium (CPM), introduced quality assessment (QA), determined the optimal cooling rate, and improved the post-thaw in vitro fertilization process. We found that the hypertonic extender E400 preserved motility of sperm held on ice for at least 6 h. We implemented …

Estimating Waterfowl Carrying Capacity At Local Scales: A Case Study From Edwin B. Forsythe National Wildlife Refuge, New Jersey, Kevin M. Ringelman, Christopher K. Williams, Paul M. Castelli, Mason L. Sieges, Rebecca A. Longenecker, Theodore C. Nichols, Stephen D. Earsom

Faculty Publications

The management of wintering North American waterfowl is based on the premise that the amount of foraging habitat can limit populations. To estimate carrying capacity of winter habitats, managers use bioenergetic models to quantify energy (food) availability and energy demand, and use results as planning tools to meet regional conservation objectives. Regional models provide only coarse estimates of carrying capacity because habitat area, habitat energy values, and temporal trends in population-level demand are difficult to quantify precisely at large scales. We took advantage of detailed data previously collected on wintering waterfowl at Edwin B. Forsythe National Wildlife Refuge and surrounding …

Dopamine-Related Genotypes And Physical Activity Change During An Intervention: The Lifestyle Interventions And Independence For Elders Study, Andrea L. Rosso, Andrea L. Metti, Nancy W. Glynn, Robert M. Boudreau, W. Jack Rejeski, Nicolaas Bohnen, Haiying Chen, Neil M. Johannsen, Abby C. King, Todd M. Manini, Marco Pahor, Stephanie A. Studenski, Carlos A.Vaz Fragoso, Caterina Rosano

Faculty Publications

Objectives: To determine whether intervention-induced physical activity (PA) changes in sedentary older adults differed according to dopamine-related genotype. Design: Randomized clinical trial (Lifestyle Interventions and Independence for Elders Trial (2010–13)). Setting: Multicenter study, 8 U.S. locations. Participants: Volunteer sample of sedentary adults aged 70 to 89 at risk of disability (N=1635). Interventions: Structured PA versus health education (HE) for an average of 2.6 years. Measurements: Single-nucleotide polymorphisms of dopamine-related genes (dopamine receptor (DR) D1, DRD2, DRD3, and catechol-O-methyltransferase (COMT)) were assessed. Average moderate to vigorous PA (MVPA) was calculated using accelerometry (min/d) at baseline and 6, 12, and 24 months. …

Biomolecular Processing Platform And Uses Thereof, Francis Barany, George Grills, Robin L. Mccarley, Collin J. Mckinney, Dorel Moldovan, Michael C. Murphy, Dimitris E. Nikitopoulos, Sunggook Park, Elizabeth J. Podlaha-Murphy, Steven A. Soper

College of Science Patents

The present invention relates to a device comprising a biomolecular processor. Each biomolecular processor has one or more bioreactor chambers defined by a solid substrate; a support structure within each bioreactor; a cleaving enzyme immobilized to the support structure and operatively positioned within the bioreactor chamber to cleave monomer or multimer units of a biopolymer molecule operatively engaged by the cleaving enzyme; and one or more time-of-flight channels formed in the solid substrate and fluidically coupled to said one or more bioreactor chambers. Each of the time-of-flight channels have two or more sensors including at least (i) a first sensor …

Host And Symbionts In Pocillopora Damicornis Larvae Display Different Transcriptomic Responses To Ocean Acidification And Warming, Emily B. Rivest, Morgan W. Kelly, Melissa B. Debiasse, Gretchen E. Hofmann

Faculty Publications

© Copyright © 2018 Rivest, Kelly, DeBiasse and Hofmann. As global ocean change progresses, reef-building corals and their early life history stages will rely on physiological plasticity to tolerate new environmental conditions. Larvae from brooding coral species contain algal symbionts upon release, which assist with the energy requirements of dispersal and metamorphosis. Global ocean change threatens the success of larval dispersal and settlement by challenging the performance of the larvae and of the symbiosis. In this study, larvae of the reef-building coral Pocillopora damicornis were exposed to elevated pCO2 and temperature to examine the performance of the coral and its …

Bending Gender In French Literature, Jacey Flatte

LSU Master's Theses

This paper looks at French literature that defies binary and heteronormative gender roles and identities. The literature spans from the seventeenth- to the nineteenth-century. My goal is not only to bring these pieces of literature to light, proving that non-binary genders have existed long before the recent liberal LGBTQIA+ movement, but also to help modernize these identities from the past. In doing this, I aim to explain that contemporary gender identities are not new, but timeless. The experiences seen in this literature are strikingly similar to how people experience gender today. As a result, I hope that readers will be …

Generating Audio Using Recurrent Neural Networks, Andrew Pfalz

LSU Doctoral Dissertations

Long Short Term Memory cells are a type of recurrent neural network that perform well when predicting sequence data. This works presents four approaches to modeling audio data. The models are trained to predict either raw audio samples or magnitude spectrum windows based on prior input audio. In a process called sampling, the models can then be employed to generate new audio using what they learned about the data they were trained on.

Four methods for sampling are presented. The first has the model predict a vector for each vector in the input. The second has the model predict one …

Experimental Evaluation Of Lignocellulosic Biomass Pyrolysis By Dielectric Heating And Thermocatalytic Upgrading Of Pyrolysis Vapors Via Iron-Doped Montmorillonite Clay Catalyst, Candice Raffaela Ellison

LSU Doctoral Dissertations

This work is an investigation of microwave-assisted biomass pyrolysis and thermocatalytic upgrading of pyrolysis vapors. In the first two chapters, the microwave susceptibility of mixtures of biomass with potential microwave absorbers are characterized by dielectric measurements at microwave frequencies. Dielectric constant and dielectric loss factor of biomass mixtures with char and biomass mixtures with bentonite are found to increase quadratically with increasing weight percent of char or bentonite.

Chapter 3 presents a finite element model, developed in COMSOL Multiphysics, comparing two waveguide applicator configurations for microwave processing using biomass as a model material. A standing wave configuration results in intense …

A Novel Multiproxy Approach To Reconstructing Sea Surface Temperature In The Southwestern Gulf Of Mexico, Marissa Anne Vara

LSU Master's Theses

Coral Sr/Ca is a widely-used proxy for sea surface temperature (SST); however, discrepancies in the coral Sr/Ca-SST relationship among colonies of the same species makes uncertainty in absolute temperature reconstructions larger than the climate signal yet climate variability studies are possible. Furthermore, the riverine input of terrestrial carbonate weathering products to the coastal waters can disrupt the coral Sr/Ca-SST signal by changing local seawater chemistry. This study evaluates the fidelity and reproducibly of coral SST proxies (Sr/Ca, Li/Ca, Mg/Li, Sr-U, Mg/Ca, and U/Ca) and a riverine-upwelling proxy (Ba/Ca) in four Orbicella faveolata colonies in two reefs offshore from Veracruz, Mexico …

Effects Of Irradiation Dose On Sperm Production, Insemination, And Male Mating Possible Period In The Sweetpotato Weevil (Coleoptera: Brentidae), Satoshi Hiroyoshi, Takayuki Mistunaga, Tsuguo Kohama, Gadi V.P. Reddy

Faculty Publications

The sterile insect technique (SIT) has been used for the control or eradication of target insect pests. To successfully apply SIT, it is very important to clarify the effect of irradiation on male reproduction in the target pest, because their mating and spermiogenesis abilities affect the success of eradication program. The sweetpotato weevil, Cylas formicarius (Fabricius) (Coleoptera: Brentidae) is a notorious and worldwide pest of sweet potato. We investigated the effect of irradiation at five doses ranging from 0 (control) to 150 Gy on 9-d-old males. Survival rate of the control (no treatment) remained high from day 10 to 20 …

The Polyphyly Of Plasmodium: Comprehensive Phylogenetic Analyses Of The Malaria Parasites (Order Haemosporida) Reveal Widespread Taxonomic Conflict, Spencer C. Galen, Janus Borner, Ellen S. Martinsen, Juliane Schaer, Christopher C. Austin, Christopher J. West, Susan L. Perkins

Faculty Publications

© 2018 The Authors. The evolutionary relationships among the apicomplexan blood pathogens known as the malaria parasites (order Haemosporida), some of which infect nearly 200 million humans each year, has remained a vexing phylogenetic problem due to limitations in taxon sampling, character sampling and the extreme nucleotide base composition biases that are characteristic of this clade. Previous phylogenetic work on the malaria parasites has often lacked sufficient representation of the broad taxonomic diversity within the Haemosporida or the multi-locus sequence data needed to resolve deep evolutionary relationships, rendering our understanding of haemosporidian lifehistory evolution and the origin of the human …

Sugarcane Cell Wall-Associated Defense Responses To Infection By Sporisorium Scitamineum, João P.R. Marques, Jeffrey W. Hoy, Beatriz Appezzato-Da-Glória, Andrés F.G. Viveros, Maria L.C. Vieira, Niranjan Baisakh

Faculty Publications

The plant cell wall is known to be the first barrier against plant pathogens. Detailed information about sugarcane cell wall-associated defense responses to infection by the causal agent of smut, Sporisorium scitamineum, is scarce. Herein, (immuno)histochemical analysis of two smut resistant and two susceptible sugarcane cultivars was conducted to understand host cell wall structural and compositional modifications in response to fungal infection. Results showed that the fungus grew on the surface and infected the outermost bud scale of both susceptible and resistant cultivars. The present findings also supported the existence of early (24 h after inoculation) and later (72-96 h …

Labor And Delivery: Television Performances By Pregnant Actresses From 1948-2016, Evleen Michelle Nasir

LSU Doctoral Dissertations

Labor and Delivery: Television Actresses’ Pregnant Performances from 1948-2016, examines the labor of six pregnant actresses working on United States television. Mary Kay Stearns, Lucille Ball, Jane Leeves, Kerry Washington, and Katey Sagal all worked through pregnancies while filming their respective television shows. These women exemplify the multitude of actresses who maintained their careers and their pregnancies in the television industry. This is the first study of its kind to examine the labor of an actresses’ pregnant body on film while she performs a role other than herself. Previous examinations of pregnancy in performance are few but have largely focused …

In The Blood: Repressing Reproduction In The French Convent From Sanguinity To Sexuality, John Stanley Patin

LSU Doctoral Dissertations

This project examines the French convent as a convergent point of evolving blood myths in the eighteenth century. According to Michel Foucault, as a society transitions from sovereign power to more liberal forms of government, the target of how power is enforced shifts as well: from the punishment of individuals who pose a threat to the crown to the regulation of perceived “deviants” who threaten the health and stability of the nation’s population. As the eighteenth century was a time of radical social change, the era encapsulates a prime moment for the study of continuities and discontinuities of these various …

Stability Of A Series Of Bodipys In Acidic Conditions: An Experimental And Computational Study Into The Role Of The Substituents At Boron, Maodie Wang, M. Graça H. Vicente, Deanna Mason, Petia Bobadova-Parvanova

Faculty Publications

© Copyright 2018 American Chemical Society. Boron-dipyrromethene (BODIPY) dyes have been extensively investigated in recent years for a variety of bioanalytical and bioimaging applications. The success of these applications relies on the stability of BODIPYs, particularly under acidic conditions. In this work, the stability of a series of 4,4′-disubstituted BODIPYs (-F, -CN, -Ph, -Me, -OMe) toward addition of excess trifluoroacetic acid (TFA) was studied systematically and comprehensively through 11B and 1H NMR, UV-vis, fluorescence, thin layer chromatography, mass spectrometry, and infrared. The results indicate that 4,4′-dicyano-BODIPY 2 is the most stable among this series and remains unchanged even 3 days …

Jane Austen's Hidden Bodies: Adaptation As Critical Commentary, Elizabeth Gilliland

LSU Doctoral Dissertations

Much of adaptation theory calls for a shift away from fidelity discourse, arguing that an adaptation does not need to be a faithful translation of the original, but should rather be viewed as literature in its own right. As such, adaptations are often studied separately from their source texts; yet even so, an unofficial hierarchy sometimes remains in deeming some types of adaptations more ripe for study than others. Further, in separating the adaptation from the original, some of the more radical alterations can be routinely dismissed as an “added” element to the text, rather than exploring what commentary these …

Multiple Origins Of Green Blood In New Guinea Lizards, Zachary B. Rodriguez, Susan L. Perkins, Christopher C. Austin

Faculty Publications

© 2018 The Authors. Several species of lizards from the megadiverse island of New Guinea have evolved green blood. An unusually high concentration of the green bile pigment biliverdin in the circulatory system of these lizards makes the blood, muscles, bones, tongue, and mucosal tissues bright green in color, eclipsing the crimson color from their red blood cells. This is a remarkable physiological feature because bile pigments are toxic physiological waste products of red blood cell catabolism and, when chronically elevated, cause jaundice in humans and all other vertebrates. Although these lizards offer a promising system to examine the evolution …

Voltage-Controlled Deposition Of Nanoparticles For Next Generation Electronic Materials, Subhodip Maulik

LSU Doctoral Dissertations

This work presents both a feasibility study and an investigation into the voltage-controlled spray deposition of different nanoparticles, namely, carbon nanotubes (CNTs), as well as molybdenum disulfide (MoS₂) and tungsten disulfide (WS₂) from the transition metal dichalcogenides (TMDCs) family of materials. The study considers five different types of substrates as per their potential application to next-generation device electronics. The substrates selected for this research were: 1) aluminum as a conducting substrate, 2) silicon as a semiconducting substrate, 3) glass, silicon dioxide (SiO₂), and syndiotactic poly methyl methacrylate (syndiotactic PMMA) as insulating substrates.

Since the …

Collaboratively Designed Customized Ethnic Dress: An Exploration Of Consumption Motivation Of First And Second Generation African Immigrant Women In Us, Jane Andayi Opiri

LSU Doctoral Dissertations

African immigrant women in the USA are wear their ethnic dress when attending social functions such as church, weddings, and parties. They work with a tailor to help them create and customize their ethnic dress. With this in mind, the study sought to explore the motivations to collaboratively customize and wear the ethnic dress among these women. A Means end chain, MEC theory (Gutman, 1997) was used as the theoretical model. To answer the research questions, a qualitative approach was utilized, using semi structure interviews with the soft laddering technique and photo elicitation methods. A convenience sample of 15 first …

Preoptic Leptin Signaling Modulates Energy Balance Independent Of Body Temperature Regulation, Sangho Yu, Helia Cheng, Marie François, Emily Qualls-Creekmore, Clara Huesing, Yanlin He, Yanyan Jiang, Hong Gao, Yong Xu, Andrea Zsombok, Andrei V. Derbenev, Eduardo A. Nillni, David H. Burk, Christopher D. Morrison, Hans Rudolf Berthoud, Heike Münzberg

Faculty Publications

© Yu et al. The adipokine leptin acts on the brain to regulate energy balance but specific functions in many brain areas remain poorly understood. Among these, the preoptic area (POA) is well known to regulate core body temperature by controlling brown fat thermogenesis, and we have previously shown that glutamatergic, long-form leptin receptor (Lepr)-expressing neurons in the POA are stimulated by warm ambient temperature and suppress energy expenditure and food intake. Here we further investigate the role of POA leptin signaling in body weight regulation and its relationship to body temperature regulation in mice. We show that POA Lepr …

Direct Determination Of Hydroxymethyl Conformations Of Plant Cell Wall Cellulose Using 1 H Polarization Transfer Solid-State Nmr, Pyae Phyo, Tuo Wang, Yu Yang, Hugh O'Neill, Mei Hong

Faculty Publications

Copyright © 2018 American Chemical Society. In contrast to the well-studied crystalline cellulose of microbial and animal origins, cellulose in plant cell walls is disordered due to its interactions with matrix polysaccharides. Plant cell wall (PCW) is an undisputed source of sustainable global energy; therefore, it is important to determine the molecular structure of PCW cellulose. The most reactive component of cellulose is the exocyclic hydroxymethyl group: when it adopts the tg conformation, it stabilizes intrachain and interchain hydrogen bonding, while gt and gg conformations destabilize the hydrogen-bonding network. So far, information about the hydroxymethyl conformation in cellulose has been …

The Effect Of Community Violence Exposure On Self-Reported Anxiety Symptoms In Adolescents: The Role Of Social Support Versus Family Cohesion, Blair Burke Kreiger

LSU Doctoral Dissertations

Exposure to community violence is a prevalent and problematic occurrence for many adolescents. Previous research associates direct (i.e., victimization) and indirect (i.e., witnessing or knowledge of incidences) community violence exposure with numerous adverse externalizing and internalizing psychological symptoms, including aggression, post-traumatic stress, depression, and anxiety. The literature also has identified risk and protective factors in community violence exposure and its outcomes. This study examined the relationship between community violence exposure and self-reported anxiety symptoms in adolescents, while also evaluating the role of social support and family cohesion as moderating variables. Results did not indicate significant relationship between community violence exposure …

The Sensitivity Of Future Ocean Oxygen To Changes In Ocean Circulation, Jaime B. Palter, David S. Trossman

Faculty Publications

No abstract provided.

Dress Rules And Body Perceptions Of Mature Plus Size Women, Megan Katherine Romans

LSU Master's Theses

Due to western societies continued obsession with youth, thinness, and fitness it is no surprise that mature women and plus size women alike are still highly stigmatized and widely ignored by the fashion industry. Past studies related to mature women and plus size women’s dress and body perception have been conducted, however, there is a severe lack of research related to mature women who are also plus size. Therefore, the purpose of this research was to understand the dress rules and body perceptions of mature plus size women (MPSW) over their lifetime. This exploratory study consisted of 16 semi-structured interviews …

Analysis Of Chromatin Interactions Of Beaf-Associated Promoters Using 4c, Shraddha Shrestha

LSU Doctoral Dissertations

A high degree of chromosome compaction is needed to fit nearly 2 meters of DNA inside a human nucleus of around 10 µm diameter. Correct chromatin folding is crucial to facilitate important nuclear functions such as transcription, DNA replication and DNA repair. Nuclei contain a variety of proteins, many of which help regulate chromatin structure and function. The mechanisms by which these proteins work are diverse and complicated. Here, we study the chromatin interactions of Boundary Element Associated Factor (BEAF) associated sites to gain insight into eukaryotic genome organization. We used circular chromosome conformation capture (4C) technology to detect genome-wide …

Uswhy Does Not The Leaf Weight-Area Allometry Of Bamboos Follow The 3/2-Power Law?, Shuyan Lin, Lijuan Shao, Cang Hui, Yu Song, Gadi V.P. Reddy, Johan Gielis, Fang Li, Yulong Ding, Qiang Wei, Peijian Shi

Faculty Publications

The principle of similarity (Thompson, 1917) states that the weight of an organismfollows the 3/2-power law of its surface area and is proportional to its volume on the condition that the density is constant. However, the allometric relationship between leaf weight and leaf area has been reported to greatly deviate from the 3/2-power law, with the irregularity of leaf density largely ignored for explaining this deviation. Here, we choose 11 bamboo species to explore the allometric relationships among leaf area (A), density (ρ), length (L), thickness (T), and weight (W). Because the edge of a bamboo leaf follows a simplified …