Digital Commons Network^™

Mechanism Of Neutrophil Homeostasis And Immunity In Pneumonia And Sepsis, Sagar Paudel

LSU Doctoral Dissertations

Severe bacterial pneumonia and septicemia are pressing health problems. A better understanding of cellular and molecular players of neutrophil immunity and homeostasis in bacterial pneumonia and sepsis is critical for inspiring novel therapeutics. Chemokine CXCL1 is a widely secreted neutrophil attractant and Nod-like receptor (NLRC4) is commonly expressed cytoplasmic pathogen sensor in hematopoietic compartments. How these innate mediators convert pathogen signals into molecular cues of immune response in context of Gram-positive bacterial pneumonia and septicemia largely remain unknown. Utilizing Cxcl1 gene deficient mice, we demonstrate CXCL1 regulates neutrophil influx, bacterial clearance, and host survival in pneumococcal pneumonia-derived sepsis. Furthermore, Cxcl1 …

The Impact Of Bridge Program Participation On The Academic Success And Retention Of Freshman Music Majors, Allie Celeste Prest

LSU Doctoral Dissertations

The bridge program for incoming music majors at Louisiana State University, run by the School of Music, is a program in its sixth year of existence (2014-2019). The program is a 5 day pre-college orientation that seeks to “bridge the gaps” for incoming music majors through a variety of programming, including sessions on note taking and lectures (given by current music faculty), peer-led integration sessions, financial and time-management workshops presented by university staff, and panels with alumni and community arts leaders. This program was developed as a departmental response to the call to increase the academic success and retention of …

Quartz Crystal Microbalance Based Sensors For Detection And Discrimination Of Volatile Organic Compounds Using Ionic Materials, Stephanie R. Vaughan

LSU Doctoral Dissertations

Volatile organic compounds (VOCs) are prevalent in everyday life, ranging from household chemicals, naturally occurring scents from common plants and animals, to industrial-scale chemicals. Many of these VOCs are known to cause adverse health and environmental effects and require regulation to prevent pollution. Detecting VOCs plays a critical role in food quality control, environmental quality control, medical diagnostics, and explosives detection. Thus, development of adequate sensing devices for detection and discrimination of VOCs is of great importance. In recent years, use of quartz crystal microbalance (QCM) based sensor arrays for analyses of VOCs has attracted significant interest. Detection of VOCs …

Thioenamide Synthesis Inspired By Peptide Macrocycles, Joshua Allen Lutz

LSU Doctoral Dissertations

Despite advances in medicine, antibiotic resistance threatens to return once preventable diseases to the human population. The microbisporicins are 24-amino acid antibiotic peptides belonging to the lantibiotic class, which pathogens have been slow to develop resistance. The uncommon post-translational modification S-[(Z)-2-aminovinyl]-D-cysteine (AviCys) is likely crucial to their activity, and appears in a small number of other peptides with compelling biological activities. Total synthesis of an AviCys-containing peptide has eluded the chemical community.

The primary challenge of AviCys synthesis is the construction of a thioenamide functional group. We demonstrate that acid-promoted reactions between an amide and …

Development Of A Tunable Platform For The Study Of Geomacromolecular Matrices Using Controlled Radical Polymerization, Arjun Pandey

LSU Doctoral Dissertations

Soil is an important environmental component, and the study of soil processes have many practical implications such as improvement in agriculture, mitigation of climate change etc. The widespread use of Agricultural Chemicals (ACs) in modern agriculture has resulted in adverse effects in environment and human health mostly through contamination into food and water sources. Study of fate, bioavailability, and transport of ACs involves molecular level understanding of their interactions with soil. This can be challenging due to complex and heterogeneous nature of soil. One common approach used is the correlation of macroscopic properties of soil, (e.g. sorption) with empirical parameters …

Visualization And Quantitation Of Radioiodine Distribution In Silver Zeolite Cartridges, Daniel Dimarco

LSU Master's Theses

Iodine 131 is a major fission product released during a nuclear incident. This isotope provides a serious health hazard for humans and the environment, therefore nuclear power plants must monitor releases using air sampling. The air sampling is accomplished using air filter cartridges using silver zeolite as a filter media. During an emergency situation, silver zeolite is useful for its affinity to I-131 without adsorbing other radioactive gases. After leaving the plume, these cartridges are counted in a low background area. This measurement does not take into account the distribution of radioiodine in the cartridge. This study explores two methods …

Purification Of Tag-Free Chlamydia Trachomatis Scc4 For Structural Studies Using Sarkosyl-Assisted On-Column Complex Dissociation, Thilini O. Ukwaththage, Octavia Y. Goodwin, Abigael C. Songok, Alexa M. Tafaro, Li Shen, Megan A. Macnaughtan

Faculty Publications

Copyright © 2019 American Chemical Society. Chlamydia trachomatis is an obligate intracellular bacterial pathogen that causes the most common sexually transmitted bacterial disease in the world. The bacterium has a unique biphasic developmental cycle with a type III secretion system (T3SS) to invade host cells. Scc4 is a class I T3SS chaperone forming a heterodimer complex with Scc1 to chaperone the essential virulence effector, CopN. Scc4 also functions as an RNA polymerase binding protein to regulate σ66-dependent transcription. Aggregation and low solubility of 6X-histidine-tagged Scc4 and the insolubility of 6X-histidine and FLAG-tagged Scc1 expressed in Escherichia coli have hindered the …

Nlrp6 In Gram-Positive Pneumonia And Sepsis, Laxman Ghimire

LSU Doctoral Dissertations

Acute lower respiratory infections (pneumonia) and pneumonia-derived sepsis are among the leading causes of death in the world causing 7.8 million deaths annually. In this regard, Methicillin-resistant Staphylococcus aureus (MRSA) is endemic in the US and implicated for causing high mortality-associated necrotizing pneumonia and aggravating viral pneumonia with superinfection. Additionally, sepsis is the 7^th leading cause of death among newborns in the US and is responsible for more than 750,000 hospitalization cases every year. Although there is a plethora of research in both pneumonia and sepsis, the detailed pathophysiology still remains elusive. Understanding the host defense mechanism will help …

Reproductive Parameters And Female Breeding Season Survival Of Rio Grande Wild Turkeys In South Central Texas, Jacob White

LSU Master's Theses

Historically, Rio Grande wild turkeys in south central Texas have been at lower densities than other portions of the state. Within the Oak-Prairie Wildlife District of Texas, Rio Grande wild turkey regulatory restrictions are different for counties in the eastern and western portions of the ecoregion. Due to perceived increases in turkey density in the eastern portion of the ecoregion (hereafter 1-bird zone), Texas Parks and Wildlife Department (TPWD) considered increasing the bag limit to match counties in the western portion of the district (hereafter 4-bird zone) in order to increase hunting opportunities. However, if regulatory changes are to be …

Ultrafast And Real-Time Dynamics Of Nanomaterials Studied By Advanced Spectroscopic Techniques, Jeewan Chaminda Ranasinghe

LSU Doctoral Dissertations

Ultrafast and nonlinear spectroscopies are used to study excited-state dynamics and monitor real-time growth dynamics of different types of nanomaterials. In the first study, the growth dynamics of colloidal gold-silver core-shell nanoparticles are studied using in situ second harmonic generation and extinction spectroscopy. The growth lifetimes are studied under different reaction conditions, resulting in different silver shell thicknesses, with spectral comparisons to finite-difference time-domain calculations. The results are consistent with a three-step growth process. During the first step of the nanoparticle growth reaction, rough and uneven surfaces are formed rapidly giving rise to plasmonic hot spots with corresponding broad, red-shifted …

Louisiana 4-H Agents' Perceptions Of The 4-H Civic Engagement Mission Mandate, Xavier Bell

LSU Master's Theses

4-H, America’s largest youth development organization, allows “youth to engage in hands-on learning in areas such as health, science, agriculture, and civic engagement in a positive environment where they receive guidance from adult mentors and are encouraged to take on proactive leadership roles” (National 4-H Council, n.d.) However, 4-H Agents are the gatekeepers to 4‑H programs because they lead educational and personal development programming that allow youth to grow and mature. As such, Louisiana 4-H Agents were the population (f=78) under study. As such, I analyzed 4-H Agents’ perceptions of the value they place on youth participating …

Characterization And Amplification Of Retrotransposable Elements Platy-1 And Alu In The Cebidae Lineage Of Platyrrhine Primates, Jessica Storer

LSU Doctoral Dissertations

Alu mobile elements are much more than “junk DNA”. Inherent properties such as high copy number, small ~300 bp size, and their nearly homoplasy-free nature make these elements particularly useful in resolving primate phylogenies. In addition, shared sequence features and identity with the Alu element allow for discovery of new SINE retrotransposons, such as the Platyrrhine-limited Platy-1 element. Building on previous research of subfamily analysis, the Platy-1 and Alu elements can be used not only to explore the controversial New World monkey (NWM) phylogeny, but also the mode and tempo of their amplification in different primate genera and species.

Chapter …

Environmentally Persistent Free Radicals: Linking Air Pollution And Poor Respiratory Health?, Peter D. Sly, Stephania A. Cormier, Slawomir Lomnicki, Jeffrey N. Harding, Keith Grimwood, Griffith University

Faculty Publications

No abstract provided.

How Protein - Protein Interactions Contribute To Pyrenoid Formation In Chlamydomonas, Ananya Mukherjee, James V. Moroney

Faculty Publications

© The Author(s) 2019. The chloroplast pyrenoid, an important component of the CO2 concentrating mechanism of algae, is a structure composed primarily of Rubisco. In Chlamydomonas, Rubisco in the pyrenoid is held together by the linker protein EPYC1. Atkinson et al., (2019) determined the regions of the Rubisco small subunit and EPYC1 that are important for the protein-protein interaction, thus making progress towards reconstruction of a pyrenoid in higher plants. Why is a protein soluble in one organism while its homologue in another species becomes part of a liquidlike cell structure? That is the question being addressed by Atkinson et …

Evaluation Of Reproductive Phenology And Ecology Of Wild Turkey (Meleagris Gallopavo) Across The Southeastern United States, Landon Robert Schofield

LSU Master's Theses

The primary driver of population growth and sustainability of gallinaceous birds is annual recruitment. Habitat selection by wild turkeys (Meleagris gallopavo spp.) during reproductive activities could influence production at multiple temporal and spatial scales. Vegetation conditions at nest sites that could drive nest success have not been clearly identified, which suggests that other factors may drive reproductive success.

Female wild turkeys maintain dominance hierarchies, which could influence how reproductively active females distribute themselves across the landscape during reproductive periods. Using high-frequency GPS data collected from reproductively active females, I analyzed nesting attempts for Eastern (n = 381), …

Ethylene And Phloem Signals Are Involved In The Regulation Of Responses To Fe And P Deficiencies In Roots Of Strategy I Plants, Carlos Lucena, Rafael Porras, María J. García, Esteban Alcántara, Rafael Pérez-Vicente, Ángel M. Zamarreño, Eva Bacaicoa, José M. García-Mina, Aaron P. Smith, Francisco J. Romera

Faculty Publications

© Copyright © 2019 Lucena, Porras, García, Alcántara, Pérez-Vicente, Zamarreño, Bacaicoa, García-Mina, Smith and Romera. Iron (Fe) and phosphorus (P) are two essential mineral nutrients whose acquisition by plants presents important environmental and economic implications. Both elements are abundant in most soils but scarcely available to plants. To prevent Fe or P deficiency dicot plants initiate morphological and physiological responses in their roots aimed to specifically acquire these elements. The existence of common signals in Fe and P deficiency pathways suggests the signaling factors must act in conjunction with distinct nutrient-specific signals in order to confer tolerance to each deficiency. …

Turn-On Near-Infrared Fluorescent Sensors For Selective Monitoring Of Kinase Activity, Gerard Thomas Ducharme

LSU Doctoral Dissertations

Reliable and selective detection of specific biopolymers is critical in a broad range of biomedical and technological areas, including availability of simple and efficient point-of-care-compatible systems for the detection of prognostic, pharmacodynamic and predictive biomarkers. Monitoring epidermal growth factor receptor (EGFR) tyrosine kinase is of major significance due to its link to many epithelial cancers. This task is complicated by the high rate of mutation occurring in patients treated with kinase inhibitors. This dissertation describes a conceptually novel design and synthesis of turn-on near-infrared fluorescent sensors for selective and sensitive detection of EGFR. The fluorescent signal generation mechanism is based …

Accumulation Over Evolutionary Time As A Major Cause Of Biodiversity Hotspots In Conifers, Mekala Sundaram, Michael J. Donoghue, Aljos Farjon, Denis Filer, Sarah Mathews, Walter Jetz, Andrew B. Leslie

Faculty Publications

© 2019 The Author(s) Published by the Royal Society. All rights reserved. Biodiversity hotspots are important for understanding how areas of high species richness form, but disentangling the processes that produce them is difficult. We combine geographical ranges, phylogenetic relationships and trait data for 606 conifer species in order to explore the mechanisms underlying richness hotspot formation. We identify eight richness hotspots that overlap known centres of plant endemism and diversity, and find that conifer richness hotspots occur in mountainous areas within broader regions of long-term climate stability. Conifer hotspots are not unique in their species composition, traits or phylogenetic …

Detecting Parasite Associations Within Multi-Species Host And Parasite Communities, Tad A. Dallas, Anna Liisa Laine, Otso Ovaskainen

Faculty Publications

© 2019 The Author(s) Published by the Royal Society. All rights reserved. Understanding the role of biotic interactions in shaping natural communities is a long-standing challenge in ecology. It is particularly pertinent to parasite communities sharing the same host communities and individuals, as the interactions among parasites—both competition and facilitation—may have far-reaching implications for parasite transmission and evolution. Aggregated parasite burdens may suggest that infected host individuals are either more prone to infection, or that infection by a parasite species facilitates another, leading to a positive parasite–parasite interaction. However, parasite species may also compete for host resources, leading to the …

Ornithological Expeditions To Sarawak, Malaysian Borneo, 2007-2017, Frederick H. Sheldon, Haw Chuan Lim, Phred M. Benham, Matthew L. Brady, Clare E. Brown, Ryan C. Burner, Vivien L. Chua, John C. Mittermeier, Subir B. Shakya, Paul Van Els, Mustafa Abdul Rahman, Dency F. Gawin, Zahirunisa Abdul Rahim, Luisa Duya Setia, Robert Moyle

Occasional Papers of the Museum of Natural Science, Louisiana State University

Louisiana State University, the University of Kansas, and the Universiti Malaysia Sarawak undertook collaborative research on the evolution and ecology of Bornean birds starting in 2005. This collaboration included a series of expeditions from 2007–2017 to collect and study birds at >30 sites in Sarawak, Malaysian Borneo. Here we provide information on the study-sites and summarize the main discoveries resulting from the collaboration.

Age And Growth Of Hardhead Catfish And Gafftopsail Catfish In Coastal Louisiana, Usa, Shane Flinn, Stephen Midway

Faculty Publications

The Hardhead Catfish Ariopsis felis and Gafftopsail Catfish Bagre marinus are marine catfishes that have low recreational and commercial landings in U.S. waters, although they are among the most abundant species in coastal waters of the Gulf of Mexico. Few biological studies exist on marine catfishes, with most studies focusing on their unique reproductive biology. This study evaluated weight-length relationships for Gafftopsail Catfish (n = 264) and Hardhead Catfish (n = 823) sampled from across coastal Louisiana, USA, during 2016-2018; subsamples (93 and 266, respectively) were aged using lapilli for age and growth analyses. Hardhead Catfish showed a small but …

Frequent Fire Reorganizes Fungal Communities And Slows Decomposition Across A Heterogeneous Pine Savanna Landscape, Tatiana A. Semenova-Nelsen, William J. Platt, Taylor R. Patterson, Jean Huffman, Benjamin A. Sikes

Faculty Publications

© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust Pyrogenic savannas with a tree–grassland ‘matrix’ experience frequent fires (i.e. every 1–3 yr). Aboveground responses to frequent fires have been well studied, but responses of fungal litter decomposers, which directly affect fuels, remain poorly known. We hypothesized that each fire reorganizes belowground communities and slows litter decomposition, thereby influencing savanna fuel dynamics. In a pine savanna, we established patches near and away from pines that were either burned or unburned in that year. Within patches, we assessed fungal communities and microbial decomposition of newly deposited litter. Soil variables and …

Pacts 1.0: A Crowdsourced Reporting Standard For Paleoclimate Data, Kristine Delong

Faculty Publications

The progress of science is tied to the standardization of measurements, instruments, and data. This is especially true in the Big Data age, where analyzing large data volumes critically hinges on the data being standardized. Accordingly, the lack of community-sanctioned data standards in paleoclimatology has largely precluded the benefits of Big Data advances in the field. Building upon recent efforts to standardize the format and terminology of paleoclimate data, this article describes the Paleoclimate Community reporTing Standard (PaCTS), a crowdsourced reporting standard for such data. PaCTS captures which information should be included when reporting paleoclimate data, with the goal of …

Hinge Region In Dna Packaging Terminase Pul15 Of Herpes Simplex Virus: A Potential Allosteric Target For Antiviral Drugs, Lana F. Thaljeh, J. Ainsley Rothschild, Misagh Naderi, Lyndon M. Coghill, Jeremy M. Brown, Michal Brylinski

Faculty Publications

Approximately 80% of adults are infected with a member of the herpesviridae family. Herpesviruses establish life-long latent infections within neurons, which may reactivate into lytic infections due to stress or immune suppression. There are nine human herpesviruses (HHV) posing health concerns from benign conditions to life threatening encephalitis, including cancers associated with viral infections. The current treatment options for most HHV conditions mainly include several nucleoside and nucleotide analogs targeting viral DNA polymerase. Although these drugs help manage infections, their common mechanism of action may lead to the development of drug resistance, which is particularly devastating in immunocompromised patients. Therefore, …

Sympathetic Innervation Of The Interscapular Brown Adipose Tissue In Mouse, Marie François, Hayden Torres, Clara Huesing, Rui Zhang, Carson Saurage, Nathan Lee, Emily Qualls-Creekmore, Sangho Yu, Christopher D. Morrison, David Burk, Hans Rudolf Berthoud, Heike Münzberg

Faculty Publications

© 2019 The Authors. Annals of the New York Academy of Sciences published by Wiley Periodicals, Inc. on behalf of New York Academy of Sciences. The recent discovery of significant brown fat depots in adult humans has revived discussion of exploiting brown fat thermogenesis in the control of energy balance and body weight. The sympathetic nervous system (SNS) has a key role in the activation of brown fat and functional mapping of its components will be crucial for the development of specific neuromodulation techniques. The mouse is an important species used for molecular genetic modulations, but its small size is …

Louisiana Agriculture Fall, 2019, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

Lsu Research Fall 2019, Louisiana State University And Agricultural And Mechanical College

Research Magazine

LSU Research is published annually by the Office of Research & Economic Development, Louisiana State University.

Ecological And Genomic Attributes Of Novel Bacterial Taxa That Thrive In Subsurface Soil Horizons, Tess E. Brewer, Emma L. Aronson, Keshav Arogyaswamy, Sharon A. Billings, Jon K. Botthoff, Ashley N. Campbell, Nicholas C. Dove, Dawson Fairbanks, Rachel E. Gallery, Stephen C. Hart, Jason Kaye, Gary King, Geoffrey Logan, Kathleen A. Lohse, Mia R. Maltz, Emilio Mayorga, Caitlin O'Neill, Sarah M. Owens, Aaron Packman, Jennifer Pett-Ridge, Alain F. Plante, Daniel D. Richter, Whendee L. Silver, Wendy H. Yang, Noah Fierer

Faculty Publications

While most bacterial and archaeal taxa living in surface soils remain undescribed, this problem is exacerbated in deeper soils, owing to the unique oligotrophic conditions found in the subsurface. Additionally, previous studies of soil microbiomes have focused almost exclusively on surface soils, even though the microbes living in deeper soils also play critical roles in a wide range of biogeochemical processes. We examined soils collected from 20 distinct profiles across the United States to characterize the bacterial and archaeal communities that live in subsurface soils and to determine whether there are consistent changes in soil microbial communities with depth across …

Allele-Specific Enhancer Interaction At The Peg3 Imprinted Domain, Joomyeong Kim, Wesley D. Frey, Kaustubh Sharma, Subash Ghimire, Ryoichi Teruyama, Lisa Stubbs

Faculty Publications

Copyright: © 2019 Kim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The parental allele specificity of mammalian imprinted genes has been evolutionarily well conserved, although its functional constraints and associated mechanisms are not fully understood. In the current study, we generated a mouse mutant with switched active alleles driving the switch from paternal-to-maternal expression for Peg3 and the maternal-to-paternal expression for Zim1. The expression levels of Peg3 and Zim1, but not …

Efficiency Of Insect-Proof Net Tunnels In Reducing Virus-Related Seed Degeneration In Sweet Potato, K. O. Ogero, J. F. Kreuze, M. A. Mcewan, N. D. Luambano, H. Bachwenkizi, K. A. Garrett, K. F. Andersen, S. Thomas-Sharma, R. A.A. Van Der Vlugt

Faculty Publications

Virus-related degeneration constrains production of quality sweet potato seed, especially under open field conditions. Once in the open, virus-indexed seed is prone to virus infection leading to decline in performance. Insect-proof net tunnels have been proven to reduce virus infection under researcher management. However, their effectiveness under farmer-multiplier management is not known. This study investigated the ability of net tunnels to reduce degeneration in sweet potato under farmer-multiplier management. Infection and degeneration were assessed for two cultivars, Kabode and Polista, grown in net tunnels and open fields at two sites with varying virus pressures. There was zero virus incidence at …