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Evolution Of Base Substitution Gradients In Primate Mitochondrial Genomes, Sameer Raina Jan 2004

Evolution Of Base Substitution Gradients In Primate Mitochondrial Genomes, Sameer Raina

LSU Master's Theses

The availability of large amounts of genetic data from the mitochondrial DNA of species has created an unprecedented opportunity for the study of evolutionary processes. Being our closest relatives on the evolutionary tree the primates are a prime candidate for the study of evolutionary processes. The availability of large amounts of genetic data from the primates allows us to study and compare results from different phylogenetic reconstruction methods and to study and trace rudimentary evolutionary processes within the primate lineage. The evolutionary process studied here is the response of the nucleotide frequency ratios to single-strandedness of sites during mitochondrial DNA …


Removal Of Toxic Organics In Wetlands: A Mechanistic Model, Luz Edith Florez Jan 2004

Removal Of Toxic Organics In Wetlands: A Mechanistic Model, Luz Edith Florez

LSU Master's Theses

The ability of removing toxic organic compounds in natural systems is important due to the capacity of these toxicants to increase risk of diseases when they are ingested by humans. This study developed a mechanistic model to estimate the removal efficiency of toxic organics in wetlands using the tanks-in-series model. Sensitivity analyses were performed for different values of the hydraulic loading rate and two kinds of wetlands: bottomland hardwood forest and freshwater marsh. It was observed the effect in the removal efficiency by the number of cell in series was principally perceived for values of N between 1 and 4; …


Optimal Rate Of Freezing Biological Systems, Sreedhar Thirumala Jan 2004

Optimal Rate Of Freezing Biological Systems, Sreedhar Thirumala

LSU Master's Theses

This thesis addresses optimization techniques for cryopreservation of biological systems. A part of the thesis (Chapter 2) reports a simplified procedure to predict the optimal rate of freezing biological systems once the cell level parameters are known a priori. The key cell level parameters investigated were, reference permeability of the membrane to water (Lpg), apparent activation energy (ELp), inactive cell volume (Vb), diameter (D), and the ratio of the available surface area for water transport to the initial volume of intracellular water (SA/WV). The simplified procedure was developed by performing a thorough analysis of the water transport model over a …


The Effects Of Reinforcement Magnitude On Functional Analysis Outcomes, Valerie Marie Volkert Jan 2004

The Effects Of Reinforcement Magnitude On Functional Analysis Outcomes, Valerie Marie Volkert

LSU Master's Theses

The functional analysis methodology developed by Iwata, Dorsey, Slifer, Bauman, and Richman (1982/1994) has been successfully used to identify the variables that maintain the problem behavior of individuals with developmental disabilities. However, in some cases, the results of functional analysis may be inconclusive. Altering parameters of reinforcement, such as the schedule, the quality, or magnitude of the reinforcer, may increase the likelihood of obtaining clear functional analysis results. Few studies have evaluated the effects of reinforcement magnitude on problem behavior even though basic findings indicate that this parameter may alter functional analysis outcomes. In fact, reinforcement magnitude has varied widely …


Solving A Locational Distribution Problem Of Non-Toxic Solid Waste On The Island Of Puerto Rico, Sandra A. Soto Jan 2004

Solving A Locational Distribution Problem Of Non-Toxic Solid Waste On The Island Of Puerto Rico, Sandra A. Soto

LSU Master's Theses

The island of Puerto Rico is confronting a crisis in waste management due to inadequate management from the local government, the decreasing number of landfills available, high population density, and paucity of places for waste disposal. This research develops a least-cost model for the disposal and transportation of non-hazardous solid waste. Location-allocation (LA) and Geographic Information Systems (GIS) software are used to analyze the efficiency of the present pattern of waste allocation and to identify a near-to-optimal assignment of waste for the landfills in operation today and the landfills that will be open by 2008. The “near-to-optimal” models obtained from …


The Effect Of Holding Bovine Oocytes In Follicular Fluid On Subsequent Fertilization And Embryonic Development, Angela Marie Klumpp Jan 2004

The Effect Of Holding Bovine Oocytes In Follicular Fluid On Subsequent Fertilization And Embryonic Development, Angela Marie Klumpp

LSU Master's Theses

The objective of Experiment 1 was to determine the effect of bovine follicular fluid (bFF) on nuclear maturation. Treatment A (Control) oocytes were stained with Hoechst-33342 immediately after aspiration from follicles, whereas, oocytes in Treatment B were held in bFF for 12 hours at 38¢ªC and then stained to determine nuclear status. No significant difference was detected between treatment groups. Results indicate that bFF inhibits resumption of meiosis. The objective of Experiment 2 was to determine the effect of bFF on embryonic development. Oocytes in Treatment A (Control) were placed into in vitro maturation (IVM) for 22 hours followed by …


Durability Of Pine Strandboard Modified With Low Molecular Weight Phenol Formaldehyde, Matthew Daniel Voitier Jan 2004

Durability Of Pine Strandboard Modified With Low Molecular Weight Phenol Formaldehyde, Matthew Daniel Voitier

LSU Master's Theses

A continuing challenge for wood and wood-based composite building materials has been durability--the power of resisting agents or influences which tend to cause changes, decay, or dissolution; lastingness. In the case of wood-based products, this often requires a resistance to biological degradation and moisture related dimensional instability (particularly in particle and flake or strand boards). Borden Chemical Company has developed a new low molecular weight phenol formaldehyde additive, PD-112, to improve durability in composite wood products. This study investigated PD-112’s contribution to durability in oriented strand board (OSB) through laboratory testing of treated southern pine strandboard panels versus untreated control …


Effect Of Cotton Aphids, Aphis Gossypii (Glover), On Cotton Plant Development And Yield Components, Robert Hankins Jones Jan 2004

Effect Of Cotton Aphids, Aphis Gossypii (Glover), On Cotton Plant Development And Yield Components, Robert Hankins Jones

LSU Master's Theses

The effect of cotton aphids, Aphis gossypii (Glover), on cotton plant development and yield was studied during 2000 and 2001. Tests were conducted at the Macon Ridge Station (Winnsboro, Louisiana) in 2000 and 2001, and at the Northeast Research Station (St. Joseph, Louisiana) in 2001. Tests were performed in irrigated and non-irrigated regimes using individual plant comparisons, micro-plots, and whole plots. In both years and at both locations, cotton aphid densities induced chlorosis, and a downward cupping of leaves during the infestation period. The insecticides, pymetrozine and thiamethoxam, were applied to control natural infestations of cotton aphids. Although pymetrozine and …


Design Exploration: Totem As Alternative For Efficient And Socially Responsive Burial, Mark Evan Bazzell Jan 2004

Design Exploration: Totem As Alternative For Efficient And Socially Responsive Burial, Mark Evan Bazzell

LSU Master's Theses

American cities are facing unprecedented development pressures. Urban populations in particular are increasing and diversifying, land as a resource is becoming more valuable, and designers/developers are challenged to creatively maximize space for all land uses. As urban populations grow, space for burial of the dead may become limited thereby prompting communities to consider alternatives to traditional burial. The increase in numbers of cremation already points to this trend. In addition to the spatial limitation issues there also exist issues of social and cultural limitation. Ethnic diversity is rapidly increasing and within each group one finds different traditions and needs regarding …


Microsatellite Instability In Squamous Cell Carcinoma And Lichen Sclerosus Of The Vulva, Sidney Joseph Marlborough Jan 2004

Microsatellite Instability In Squamous Cell Carcinoma And Lichen Sclerosus Of The Vulva, Sidney Joseph Marlborough

LSU Master's Theses

The usefulness of detecting genomic instability in the form of microsatellite instability (MSI) has been examined in a number of cancers. Squamous cell carcinoma (SCC) and adjacent normal and lichen sclerosis (LS) samples were taken from patients with radical vulvectomies. Vulvar SCC samples were compared with adjacent normal samples using four oligonucleotide markers (BAT-25, BAT-26, D10S208 and D10S587) found to be informative with other carcinomas. Using polymerase chain reaction techniques, 30 vulvar SCC DNA samples were examined for MSI. BAT-26 displayed the highest level of MSI and was considered to be the most sensitive marker in studying vulvar SCC. There …


Accelerating The Transition To A Sustainable Society, Christopher Brian Bennett Jan 2004

Accelerating The Transition To A Sustainable Society, Christopher Brian Bennett

LSU Master's Theses

As human populations increase, available land and resources decrease, and we begin to better understand the impacts of human activity on the environment, a strategy for human development that meets both the needs of society and the environment is being increasingly called upon. This movement has come to be known as sustainability. While this term is prevalent in the design and planning communities, it is important that this concept be brought to the attention of the general public, whom will ultimately decide the success or failure of this scheme. The question then is how to begin implementation of sustainable practices, …


Parallel Molecular Dynamics Simulations Of Antimicrobial Peptides, Svetlana V. Oard Jan 2004

Parallel Molecular Dynamics Simulations Of Antimicrobial Peptides, Svetlana V. Oard

LSU Master's Theses

Molecular dynamics (MD) simulations of the wheat antimicrobial peptide â-purothionin were carried out in explicit water (~14,000 atoms) using an all-atom model. The structural properties of the peptide as a function of MD simulation, temperature, and presence of the mono- and divalent metal ions were investigated. The accuracy and scalability tests of the code Peach 3.8 for our system were performed on the Intel Xeon-based Linux cluster SuperHelix at Louisiana State University (LSU). The code showed the reliable accuracy and the parallel efficiency of 0.61 for ~14,000 particles on 16 processors. The microcanonical (MC) MD simulation of â-purothionin in water …


Cryopreservation Of Bovine And Caprine Oocytes By Vitrification, Sabrina Marie Luster Jan 2004

Cryopreservation Of Bovine And Caprine Oocytes By Vitrification, Sabrina Marie Luster

LSU Master's Theses

Cryopreservation of animal oocytes will permit germplasm of valuable or unique females to be preserved for extended times. The objective of this research was to derive a procedure to cryopreserve bovine oocytes by vitrification to be used as recipients for somatic cell nuclear transfer (SCNT). Caprine oocytes vitrified by the same procedure were assayed by cytological examination of microtubules. In the first two of three experiments, bovine oocytes matured in vitro were vitrified in a mixture of ethylene glycol (EG), dimethylsulfoxide (Me2SO) and trehalose, and then subjected to in vitro fertilization (IVF) or SCNT. For vitrification, oocytes were first exposed …


The Fate Of Bifenthrin And Fipronil In Pine Bark Nursery Media, Russell Stanley Harris, Iii Jan 2004

The Fate Of Bifenthrin And Fipronil In Pine Bark Nursery Media, Russell Stanley Harris, Iii

LSU Master's Theses

Concerns over the adverse effects of pesticides on human health and the environment have led to the development of stricter pesticide regulations and the outright banning of many pesticides in the U.S. Bifenthrin and fipronil are important pesticides used in the nursery industry for the control of imported fire ants (Solenopsis invicta Buren, S. richteri Forel, and their hybrids) to meet the requirements of the Imported Fire Ant Quarantine. Nurseries typically use highly-organic media ("soilless" media) and little is known about the fate of these pesticides when used with these media. Our research measured the influence of irrigation frequency and …


Deterrence Factors For Copyright Infringement Online, Nico Nergadze Jan 2004

Deterrence Factors For Copyright Infringement Online, Nico Nergadze

LSU Master's Theses

The purpose of this study was to investigate deterrence factors for online file-sharing by analyzing different conditions that affect compliance with the law through survey of the students in a large university in Southern U.S. The findings show that certainty of punishment, stigma of the label, knowledge of the laws and consensus with the rule negatively correlated with both actual and likely future file-sharing activities of the users


Being Korean And Being Christian: Identity Making In The Korean Baptist Church Of Baton Rouge In The U.S. Deep South, Hyeon Ju Lee Jan 2004

Being Korean And Being Christian: Identity Making In The Korean Baptist Church Of Baton Rouge In The U.S. Deep South, Hyeon Ju Lee

LSU Master's Theses

The post-1965 generation Korean immigrants in the U.S., who have left their country for betterment of their lives, find themselves unable to acculturate to the U.S. mainstream culture. Although legally Americans, these Koreans strive to hold onto their culture they brought with them. A group of Koreans who belong to this post-1965 immigrant generation in Baton Rouge established a church to share religious and cultural experience while speaking Korean language and sharing Korean food--The Korean Baptist Church of Baton Rouge. The members of the Korean Baptist Church of Baton Rouge ("the Church") create a familial community within Christian and Confucian …


Microelectrophoresis System Utilizing Conductivity Detection Analyzing Biological Molecues, Michelle Galloway Jan 2004

Microelectrophoresis System Utilizing Conductivity Detection Analyzing Biological Molecues, Michelle Galloway

LSU Doctoral Dissertations

Microfabrication technology has proven to be a valuable tool for creating polymer-based devices utilized in chemical and biochemical assays. Although, reducing the size of the device allows for short analysis times and reduces the reagent demand to ultrasmall volumes (< 1 nanoliter), a resulting consequence is the constraint placed on the limits of detection associated with the detector hardware required for readout. To overcome such constraints, laser-induced fluorescence (LIF) is often employed as a detection method as it provides low detection limits, which approach the single molecule level. Unfortunately, most LIF systems do not offer the benefits of miniaturization, with the detector components (i.e. laser, optics, filters) often times requiring a much larger footprint compared to the device. Another readout strategy that has shown promise for these devices is conductivity detection. Detection can be accomplished using either conventional-size or microfabricated electrodes, which can be integrated on the device. Although conductivity has been commonly used to detect inorganic or small organic species, the potential for detection of biological species has received little attention. In this work, an integrated conductivity detector was developed for the analysis of amino acids, peptides, proteins, and oligonucleotides (double-stranded DNA). Using the detector, mass detection sensitivities in the range of 10-18 - 10-21 moles were achieved. To increase the throughput of the system a state-of-the-art, multichannel device with a conductivity array detector was devised. This device, which consists of a 16-channel fluidic network and a printed circuit board, is geared toward automating three-processing steps onto a single fluidic platform including purification, preconcentration and detection for downstream parallel processing.


Quality Assurance Testing Of The Eclox-M In Detecting Terrorism Threats In Louisiana's Public Drinking Water Systems, Jessica Coleman Jan 2004

Quality Assurance Testing Of The Eclox-M In Detecting Terrorism Threats In Louisiana's Public Drinking Water Systems, Jessica Coleman

LSU Master's Theses

A quality assurance testing of the ECLOX-M™, a rapid water quality indicator used in potential terrorist attacks, was evaluated for its adequacy in nine regional Louisiana water systems. The ECLOX-M™ system uses enhanced chemiluminescence to measure toxicity in water by integration of a mixture of luminol (C8H7O3N3), and an oxidant in the presence of a catalyst enzyme - horseradish peroxidase (HRP). Water samples were tested for chemiluminescence, chlorine content, pH, and arsenic and/or nerve agent/pesticide contamination. In addition, three toxic industrial chemical standards were evaluated (mercury, a volatile liquid mixture, and a …


Evaluation Of Growth, Production And Cold Tolerance Of Four Varieties Of Tilapia, Patricio Enrique Paz Jan 2004

Evaluation Of Growth, Production And Cold Tolerance Of Four Varieties Of Tilapia, Patricio Enrique Paz

LSU Master's Theses

As global tilapia production grows, it is important to characterize varieties available for production and generate data on environmental tolerances. This study generated data on tolerances, adaptabilities and production characteristics of four tilapia varieties. Goals of this study were to: (1) compare growth of four tilapia varieties in two types of recirculating systems, (2) evaluate juvenile growth of seven tilapia varieties in fresh and brackishwater mesocosms, and (3) estimate cold tolerance of juveniles of four tilapia varieties in fresh and brackishwater. Nile tilapia (Oreochromis niloticus), blue tilapia (Oreochromis aureus), Mississippi commercial strain tilapia (MCS), and Florida red tilapia (FRT) were …


Optimization Of Agrobacterium Mediated Cotton Transformation Using Shoot Apices Explants And Quantitative Trait Loci Analysis Of Yield And Yield Component Traits In Upland Cotton (Gossypium Hirsutum), Baogong Jiang Jan 2004

Optimization Of Agrobacterium Mediated Cotton Transformation Using Shoot Apices Explants And Quantitative Trait Loci Analysis Of Yield And Yield Component Traits In Upland Cotton (Gossypium Hirsutum), Baogong Jiang

LSU Doctoral Dissertations

Cotton (Gossypium spp) is an important world crop. Although great improvements have been achieved through traditional breeding methods, cotton breeders are facing many problems, i.e., narrow genetic base, inability to use alien genes and difficulty in breaking gene linkages. Genetic transformations and quantitative trait loci (QTL) analyses are main tools used by breeders to overcome these problems. In this dissertation, an optimized cotton regeneration system from shoot apices was developed. The regeneration rate was increased to 85% by combining rooting induction, indole acetic acid (IAA) shock and graft techniques. The regeneration system is genotype-independent and the whole process takes 12 …


Development Of Chicken Polyclonal And Mouse Monoclonal-Based Enyzyme Immunoassays For The Detection Of Β-Cyclocitral In Catfish Pond Water, Cate Nakaweesa Munene Jan 2004

Development Of Chicken Polyclonal And Mouse Monoclonal-Based Enyzyme Immunoassays For The Detection Of Β-Cyclocitral In Catfish Pond Water, Cate Nakaweesa Munene

LSU Doctoral Dissertations

The Catfish industry faces a problem of off-flavors due to odorous compounds produced by cyanobacteria and blue-green algae. Beta-Cyclocitral imparts a hay-woody odor to pond water and fish tissues. At present there are no reliable pond treatment methods available to control these off-flavors. To monitor the levels of this compound for quality control, rapid, sensitive and inexpensive methods are needed. The major goal of this study was to develop enzyme-linked immunoflow assays based on monoclonal and polyclonal antibodies that are specific and sensitive enough to detect beta-cyclocitral in catfish pond water. Beta-Cyclocitral-PPD conjugate was prepared and used to immunize two …


Genetics And Functions Of Herpes Simplex Virus Type 1 Membrane Proteins In Virus-Induced Cell Fusion, Virion Morphogenesis And Egress, Jeffrey Michael Melancon Jan 2004

Genetics And Functions Of Herpes Simplex Virus Type 1 Membrane Proteins In Virus-Induced Cell Fusion, Virion Morphogenesis And Egress, Jeffrey Michael Melancon

LSU Doctoral Dissertations

The Herpes Simplex Virus life cycle contains a number of membrane fusion events that must function properly to ensure a productive infection, including: virus attachment and entry into susceptible cells, de-envelopment at the outer nuclear lamellae, and virus-induced cell-to-cell fusion. A virus-free cell fusion assay was recently developed in order to attempt to understand the underlying mechanisms that are responsible for viral fusion events and was utilized in order to investigate the effect of mutations targeted to the carboxyl terminus of gB. We showed that the predicted alpha helical domain H17b within the carboxyl-terminus of gB is involved in both …


Microhabitat Distribution And Demography Of Two Florida Scrub Endemic Plants With Comparisons To Their Habitat-Generalist Congeners, Satya Maliakal Witt Jan 2004

Microhabitat Distribution And Demography Of Two Florida Scrub Endemic Plants With Comparisons To Their Habitat-Generalist Congeners, Satya Maliakal Witt

LSU Doctoral Dissertations

I evaluated hypotheses regarding the nature of habitat specialization by comparing the microhabitat distribution and demography of L. cernua and P. basiramia, two Florida rosemary scrub habitat specialist species, with their habitat generalist congeners, L. deckertii and P. robusta. Specifically, I addressed the following two hypotheses: (1) that habitat specialist species may occur in a narrower range of microhabitat conditions than habitat generalist species, and (2) that demographic parameters of habitat specialist species may be more variable than those of their habitat generalist congeners. For each pair of congeners, I compared the microhabitat distributions, variation in vital rates and population …


Tuning Polymeric Chiral Surfactants For Enhanced Enantiomeric Recognition In Micellar Electrokinetic Chromatography, Jepkoech Tarus Jan 2004

Tuning Polymeric Chiral Surfactants For Enhanced Enantiomeric Recognition In Micellar Electrokinetic Chromatography, Jepkoech Tarus

LSU Doctoral Dissertations

The research presented in this dissertation involves the synthesis, modification, characterization, and the application of polymeric surfactants in micellar electrokinetic chromatography (MEKC). Sodium undecenoyl-L-Leucinate (L-SUL) was synthesized and modified by use of alkali metal counterions, alcohols, and Triton X-102. Characterization of the modified poly-L-SUL was performed in order to elucidate the structural changes on the surfactants by use of four techniques. While surface tensiometry was used for the determination of the critical micelle concentration (cmc), fluorescence measurements were used for the determination of the polarity of the modified polymeric surfactants. The information about the size of the polymeric surfactants was …


Molecular Systematics Of The Cashew Family (Anacardiaceae), Susan Katherine Pell Jan 2004

Molecular Systematics Of The Cashew Family (Anacardiaceae), Susan Katherine Pell

LSU Doctoral Dissertations

Anacardiaceae Lindl., the cashew family, is an economically important, primarily pantropically distributed family of 82 genera and over 700 species. This family is well known for its cultivated edible fruits and seeds (mangos, pistachios, and cashews), dermatitis causing taxa (e.g., Comocladia, Metopium, Semecarpus, Toxicodendron, etc.), and lacquer plants (Toxicodendron and Gluta spp.). The taxonomy of Anacardiaceae has not been thoroughly investigated since Engler established the currently used five tribal classification system over 100 years ago. This study evaluated evolutionary relationships of the family using nrDNA and cpDNA sequences. The first part of the study investigated the evolutionary position of Anacardiaceae …


Statistical Models And Genetic Evaluation Of Binomial Traits, Jose Lucio Guerra Jan 2004

Statistical Models And Genetic Evaluation Of Binomial Traits, Jose Lucio Guerra

LSU Doctoral Dissertations

Generalized mixed model methodology and MCMC simulations were used to estimate genetic parameters for calving rate and calf survival with the normal, probit, and logistic models. Calving rate and calf survival were defined as 0 each time a cow failed to calf or a calf failed to survive to weaning age, otherwise they were set to 1. Data were available on 1,458 cows and on 5,015 calves. Cows produced a total of 4,808 records over 4 discrete generations of rotational crosses between Angus, Brahman, Charolais, and Hereford from 1977 to 1995. The heritability of calving rate and calf survival, the …


Ctp: Phosphoethanolamine Cytidylytransferase And Dag: Cdp-Ethanolamine Ethanolaminephosphotransferase In The Cdp-Ethanolamine Pathway Of Chlamydomonas Reinhardtti, Wenyu Yang Jan 2004

Ctp: Phosphoethanolamine Cytidylytransferase And Dag: Cdp-Ethanolamine Ethanolaminephosphotransferase In The Cdp-Ethanolamine Pathway Of Chlamydomonas Reinhardtti, Wenyu Yang

LSU Doctoral Dissertations

Chlamydomonas reinhardtii Dangeard does not have two major phospholipids, PS and PC. This fact renders C. reinhardtii a desirable system for investigations of the PE biosynthetic pathway and its regulation independent of PC and PS biosynthesis. The cDNA coding for ECT protein in C. reinhardtii was cloned from a cDNA library. The ECT cDNA encodes a protein of 443 amino acid residues. The ECT protein in C. reinhardtii has a repetitive internal sequence in its N- and C-terminal halves. Each repeat half of the protein has a HXGH motif, a site considered to be in the catalytic domain. The protein …


Rates Of Molecular Evolution And Their Application To Neotropical Avian Biogeography, Christopher C. Witt Jan 2004

Rates Of Molecular Evolution And Their Application To Neotropical Avian Biogeography, Christopher C. Witt

LSU Doctoral Dissertations

The tempo of evolution and the causes of rate variation among lineages are central foci of evolutionary biology. I evaluated two hypothesized sources of variation in molecular evolutionary rate, and I applied a variable molecular clock to estimate the timescale of diversification in three families of Neotropical birds. First, I examined the phylogenetic evidence for molecular punctuated equilibrium, the hypothesis that speciation drives accelerated molecular evolution. Recent findings that rates of DNA evolution and speciation are linked implicate molecular punctuated equilibrium as an important cause of rate variation among lineages. I used phylogenetic simulations to test this reported link, and …


Numerical Methods For The Unsteady Incompressible Navier-Stokes Equations And Their Application To The Direct Numerical Simulation Of Turbulent Flows, Frank Herbert Muldoon Jan 2004

Numerical Methods For The Unsteady Incompressible Navier-Stokes Equations And Their Application To The Direct Numerical Simulation Of Turbulent Flows, Frank Herbert Muldoon

LSU Doctoral Dissertations

Two new methods for the efficient parallel computation of the unsteady incompressible Navier-Stokes equations are presented. Such efficient methods are desired for large scale parallel computations of unsteady turbulent flows such as Direct Numerical Simulations (DNS). The performance of the new methods has a distinct advantage over the artificial compressibility method, in that the methods exhibit robust convergence for a variety of flow problems without extensive need for tuning computational parameters. These methods and others have been implemented in a computer program designed for massively parallel computer architectures, written by the author and used to obtain all results in this …


The Production Of A Dextran Binding Antibody By Phage Display Library And Its Applications To Sugar Processing, Duwoon Kim Jan 2004

The Production Of A Dextran Binding Antibody By Phage Display Library And Its Applications To Sugar Processing, Duwoon Kim

LSU Doctoral Dissertations

A phage collection (M1114), which is a mixture of two collections, M710-3R and Silica 426 each derived from a human synthetic phage antibody library (Fab 2lox), was used further enriched for dextran binding using enzyme-linked immunosorbent assays (ELISA) screening. The effects of dextran concentration on phage binding affinity were tested using indirect sandwich ELISA on phage collections, M1114-m74 and AE-M1114-m74-2R. Most of the phage bound to dextran (T2000) coated on a sandwich ELISA. The combination of ELISA screening and a sephadex columns enriched dextran binding 7 fold over enrichment by a single ELISA screen. Phage collection (M1114-m74) produced by combination …