Digital Commons Network^™

Transitioning From A Research Protocol To A Scalable Applied Pathway For Xenopus Laevis Sperm Cryopreservation At A National Stock Center: The Effect Of Cryoprotectants, Lucía Arregui, Jack C. Koch, Terrence R. Tiersch

Faculty Publications

Sperm cryopreservation is a critical tool for safeguarding and managing valuable genetic resources. Protocols for cryopreservation of Xenopus laevis sperm were available but lacking sperm quality evaluation and scalability and the outcomes were inconsistent. The goal of this study was to begin developing a center-level cryopreservation pathway for this species by integrating French straws as containers that would facilitate germplasm repository development. The objectives were to analyze the effect of: (1) three sperm concentrations (33, 50, and 100 × 106 sperm/mL) on post-thaw fertilization, (2) three final concentrations (2.5%, 5%, and 10%) of dimethyl sulfoxide, methanol, and dimethylformamide (DMFA) on …

Song And Genetic Divergence Within A Subspecies Of White-Crowned Sparrow (Zonotrichia Leucophrys Nuttalli), Amy Rongyan Luo, Sara Lipshutz, Jennifer Phillips, Robb T. Brumfield, Elizabeth Perrault Derryberry

Faculty Publications

Animal culture evolves alongside genomes, and the two modes of inheritance-culture and genes-interact in myriad ways. For example, stable geographic variation in culture can act as a reproductive barrier, thereby facilitating genetic divergence between "cultural populations."White-crowned sparrows (Zonotrichia leucophrys) are a well-established model species for bird song learning and cultural evolution, as they have distinct, geographically discrete, and culturally transmitted song types (i.e., song dialects). In this study, we tested the hypothesis that divergence between culturally transmitted songs drives genetic divergence within Nuttall's white-crowned sparrows (Z. l. nuttalli). In accordance with sexual selection theory, we hypothesized that cultural divergence between …

Influence Of Food Security Status And Diet Quality On Maternal Gestational Weight Gain, Joshua R. Sparks, Candice A. Myers, Suzanne Phelan, Robert L. Newton, Shengping Yang, Leanne M. Redman

Clinical Research Faculty Publications

Background: Recommended gestational weight gain (GWG) is crucial for health of women and their offspring. Food security status is hypothesized to influence diet quality and GWG. Therefore, we examined the relationship between diet quality and GWG by food security status. Methods: Participants (n = 679) were enrolled in the Initial Vanguard Study of the National Children's Study. GWG was calculated as third trimester weight minus prepregnancy weight. Food security status and diet quality (Healthy Eating Index [HEI]-2015) were assessed using the Household Food Security Survey and a Diet History Questionnaire, respectively. General linear models evaluated the relationship between GWG and …

Associations Of Epigenetic Age Acceleration With Cvd Risks Across The Lifespan: The Bogalusa Heart Study, Xiao Sun, Wei Chen, Alexander C. Razavi, Mengyao Shi, Yang Pan, Changwei Li, Maria Argos, Brian T. Layden, Martha L. Daviglus, Jiang He, Owen T. Carmichael, Lydia A. Bazzano, Tanika N. Kelly

Clinical Research Faculty Publications

Although epigenetic age acceleration (EAA) might serve as a molecular signature of childhood cardiovascular disease (CVD) risk factors and further promote midlife subclinical CVD, few studies have comprehensively examined these life course associations. This study sought to test whether childhood CVD risk factors predict EAA in adulthood and whether EAA mediates the association between childhood CVD risks and midlife subclinical disease. Among 1,580 Bogalusa Heart Study participants, we estimated extrinsic EAA, intrinsic EAA, PhenoAge acceleration (PhenoAgeAccel), and GrimAge acceleration (GrimAgeAccel) during adulthood. We tested prospective associations of longitudinal childhood body mass index (BMI), blood pressure, lipids, and glucose with EAAs …

Complementing Model Species With Model Clades, Makenzie E. Mabry, R. Shawn Abrahams, Ihsan A. Al-Shehbaz, William J. Baker, Simon Barak, Michael S. Barker, Russell L. Barrett, Aleksandra Beric, Samik Bhattacharya, Sarah B. Carey, Gavin C. Conant, John G. Conran, Maheshi Dassanayake, Patrick P. Edger, Jocelyn C. Hall, Yue Hao, Kasper P. Hendriks, Julian M. Hibberd, Graham J. King, Daniel J. Kliebenstein, Marcus A. Koch, Ilia J. Leitch, Frederic Lens, Martin A. Lysak, Alex C. Mcalvay, Michael T.W. Mckibben, Francesco Mercati, Richard C. Moore, Klaus Mummenhoff, Daniel J. Murphy, Lachezar A. Nikolov, Michael Pisias, Eric H. Roalson

Faculty Publications

Model species continue to underpin groundbreaking plant science research. At the same time, the phylogenetic resolution of the land plant tree of life continues to improve. The intersection of these 2 research paths creates a unique opportunity to further extend the usefulness of model species across larger taxonomic groups. Here we promote the utility of the Arabidopsis thaliana model species, especially the ability to connect its genetic and functional resources, to species across the entire Brassicales order. We focus on the utility of using genomics and phylogenomics to bridge the evolution and diversification of several traits across the Brassicales to …

The Sequential Direct And Indirect Effects Of Mountain Uplift, Climatic Niche, And Floral Trait Evolution On Diversification Dynamics In An Andean Plant Clade, Agnes S. Dellinger, Laura Lagomarsino, Fabián Michelangeli, Stefan Dullinger, Stacey D. Smith

Faculty Publications

Why and how organismal lineages radiate is commonly studied through either assessing abiotic factors (biogeography, geomorphological processes, and climate) or biotic factors (traits and interactions). Despite increasing awareness that both abiotic and biotic processes may have important joint effects on diversification dynamics, few attempts have been made to quantify the relative importance and timing of these factors, and their potentially interlinked direct and indirect effects, on lineage diversification. We here combine assessments of historical biogeography, geomorphology, climatic niche, vegetative, and floral trait evolution to test whether these factors jointly, or in isolation, explain diversification dynamics of a Neotropical plant clade …

Cellulose Nanofiber As Mask/Personal Protective Equipment Surface Agent For Enhanced Anti-Bacterial Performance, Sophia Jackson, Jonathan Chen

Faculty Publications

The utilization of face-covering masks as an extended form of personal protective equipment has led to exponential waste measures during the COVID-19 pandemic, with estimations of up to 7200 tons of medical-type waste daily. A primary cause of this waste is surgical layered disposable masks that are constructed by melt-blown nonwovens usually made of non-biodegradable thermoplastic polymers like polypropylene. To increase widespread sustainable options to the public, commercialized or do-it-yourself-based fabric masks serve as a solution, but their resistance to harmful molecules is less than that of the medical-grade masks due to the fabric structure that leaves space for penetration. …

Cpeb3 Maintains Developmental Competence Of The Oocyte, Lucie Lamacova, Denisa Jansova, Zongliang Jiang, Michal Dvoran, Daria Aleshkina, Rajan Iyyappan, Anna Jindrova, Heng Yu Fan, Yuxuan Jiao, Andrej Susor

Faculty Publications

Mammalian oocyte development depends on the temporally controlled translation of maternal transcripts, particularly in the coordination of meiotic and early embryonic development when transcription has ceased. The translation of mRNA is regulated by various RNA-binding proteins. We show that the absence of cytoplasmic polyadenylation element-binding protein 3 (CPEB3) negatively affects female reproductive fitness. CPEB3-depleted oocytes undergo meiosis normally but experience early embryonic arrest due to a disrupted transcriptome, leading to aberrant protein expression and the subsequent failure of embryonic transcription initiation. We found that CPEB3 stabilizes a subset of mRNAs with a significantly longer 3’UTR that is enriched in its …

Editorial Introduction: Exploring The Frontier Of Policy Theory Research, Geoboo Song, Melissa K. Merry, Saba Siddiki, Gwen Arnold, Aaron Smith-Walter, Holly L. Peterson, Creed Tumlison, Heasun Choi, Briana Huett, Davor Mondom, Christopher Giller, Meerim Seiitova

Faculty Publications

No abstract provided.

Laser-Induced Breakdown Spectroscopy Analysis Of Tourmaline: Protocols, Procedures, And Predicaments, Nancy J. Mcmillan, Barbara L. Dutrow

Faculty Publications

Laser-induced breakdown spectroscopy (LIBS) is an appropriate choice of analytical tool for analysis of complex minerals because it is rapid, requires little sample preparation, and acquires major and trace element compositional information on all naturally occurring elements at concentrations above their intrinsic levels of detection for the specific analyte material. Tourmaline, a complex borosilicate mineral supergroup, was chosen as a test mineral due to the complexity of its major and minor element composition. Four analytical issues were investigated during project development: (1) the spacing between analytical laser shots to avoid analysis of the recast from previous laser ablations, (2) the …

60th Student Senate Org Session 2 Meeting Minutes, Zoe Joubert

Student Senate Meeting Minutes

No abstract provided.

60th Student Senate Org Session 1 Meeting Minutes, Zoe Joubert

Student Senate Meeting Minutes

No abstract provided.

59th Student Senate Week 10 Meeting Minutes, Zoe Joubert

Student Senate Meeting Minutes

No abstract provided.

Ammonium Chemotaxis In Chlamydomonas Reinhardtii, Gabela Nelson

LSU Doctoral Dissertations

Analysis of ammonium chemotaxis in Chlamydomonas reinhardtii is largely hindered, compared to that of phototaxis, despite equal importance on flagellated microalgal physiology. A major contribution of this shortfall is the lack of proper assay method. We developed a simple Petri dish assay method in which light is homogenously exposed while patterns of the cellular migration are tracked with a function of time. Using the method, new findings were revealed. First, this research presented that a strain lacking the eyespot organelle required for light gradient-sensing exhibits similar chemotactic behavior compared to a wild-type strain, suggesting Chlamydomonas sense an ammonium gradient not …

Human-Environment Interactions In Coastal Belize: Insights Into Sea-Level Rise And Ancient Maya Activities At The Paynes Creek Salt Works, Cheryl Mae Foster

LSU Doctoral Dissertations

Dissertation excavations were carried out in 2019 and 2022 at the underwater sites of Ta’ab Nuk Na and Ek Way Nal in Paynes Creek National Park, Belize. Sea-level rise submerged the sites in antiquity and preserved the artifact record in red mangrove (Rhizophora mangle) peat. Red mangrove peat can be used as a proxy for sea-level rise. The goals of the two excavations included: 1) excavating a 14m-long transect within and extending beyond building J at Ta’ab Nuk Na to determine the function of the building, 2) investigating the production of salt making pottery at Ta’ab Nuk Na; …

Community-Level Post-Hazard Functionality Methodology For Buildings Exposed To Floods, Omar Nofal, Nathanael Rosenheim, Sabarethinam Kameshwar, Jayant Patil, Xiangnan Zhou, John W. Van De Lindt, Leonardo Duenas-Osorio, Eun Jeong Cha, Amin Endrami, Elaina Sutley, Harvey Cutler, Tao Lu, Chen Wang, Hwayoung Jeon

Faculty Publications

This paper presents a building-level post-hazard functionality model for communities exposed to flood hazards including the interdependencies between the population, buildings, and infrastructure. An existing portfolio of building archetypes is used to model the post-hazard physical flood functionality of different building typologies within the community with the goal of supporting resilience-informed decision-making. Specific fragility-based flood functionality curves were developed for this portfolio to quantify the exceedance probability of a prescribed set of functionality states. While the physical functionality of buildings is significant to the total functionality of a building and community resilience assessment, the functionality of utilities such as power …

Generating Rate Features For Mobile Applications, Shristi Shrestha, Anas Mahmoud

Faculty Publications

Mobile application (app) stores employ standardized mechanisms for rating hosted apps, typically in the form of free text reviews and numerical rating scales. App users use these mechanisms to express their opinions about their apps and discover apps that fit their specific needs. However, existing app rating systems do not take into account the operational characteristics of application domains. Thus, generated user reviews are often short, subjective, and one-dimensional. To overcome these limitations, in this paper, we propose a multi-dimensional rating system for mobile apps. Our assumption is that an adaptive goal-based app rating system can prompt users to generate …

Blocks? Graphs? Why Not Both? Designing And Evaluating A Hybrid Programming Environment For End-Users, Nico Ritschel, Felipe Fronchetti, Reid Holmes, Ronald Garcia, David C. Shepherd

Faculty Publications

Many modern end-user development environments support one of two visual modalities: block-based programming or data-flow programming. In this work, we investigate the trade-offs between the two modalities in the context of robotics tasks. These often contain both aspects that are better solved with blocks and others that best fit data-flow programming. To address this style of task, we present and discuss two novel programming environment prototypes, one purely block-based and one a hybrid of blocks and data-flow programming. We compare the designs through a controlled experiment with 113 end-user participants, in which we asked them to solve programming and program …

Improved Modeling And Application Of Data Assimilation In Gas Influx Management During Managed Pressure Drilling, Chen Wei

LSU Doctoral Dissertations

The management of gas influxes in a drilling well has been considered one of the most critical topics for maintaining petroleum drilling safety and efficiency. Reliable modeling of gas influx behaviors in a wellbore or a marine riser greatly benefits the improved understanding of a gas kick event and the development of optimized influx management strategies. However, the current studies on transient multiphase flow modeling show varying limitations on both the model rigorousness and the overall numerical efficiency. Meanwhile, the existence of great system uncertainties, such as the unclear influx sizes and distributions in the well/riser, has brought significant challenges …

Lithium Ion Speciation In Cyclic Solvents: Impact Of Anion Charge Delocalization And Solvent Polarizability, Ernest O. Nachaki, Daniel G. Kuroda

Faculty Publications

The increasing demand for lithium batteries has triggered the search for safer and more efficient electrolytes. Insights into the atomistic description of electrolytes are critical for relating microscopic and macroscopic (physicochemical) properties. Previous studies have shown that the type of lithium salt and solvent used in the electrolyte influences its performance by dictating the speciation of the ionic components in the system. Here, we investigate the molecular origins of ion association in lithium-based electrolytes as a function of anion charge delocalization and solvent chemical identity. To this end, a family of cyano-based lithium salts in organic solvents, having a cyclic …

Enhancement Of Applied Rice Breeding Programs: Exploring Factors Impacting Genomic Selection Accuracy And Discovery Of A Novel Gene For Grain Shape, Maria G. Montiel

LSU Doctoral Dissertations

Plant breeding is a crucial tool in addressing global challenges such as climate change, population growth, and the need for more resilient crops. Rice serves as a staple food for a significant portion of the global population and has served as a model crop for genetic and genomic studies. Genomic selection (GS) has become valuable tool in plant breeding, allowing breeders to predict breeding lines’ performance based on their genotypes. The training (TS) set is a central component of genomic selection and the optimization of the training set is crucial for efficient implementation of genomic selection. The first two objectives …

Surfactant-Specific Ai-Driven Molecular Design: Integrating Generative Models, Predictive Modeling, And Reinforcement Learning For Tailored Surfactant Synthesis, Miriam Nnadili, Andrew N. Okafor, Teslim Olayiwola, David Akinpelu, Revati Kumar, Jose A. Romagnoli

Faculty Publications

Molecular design is a critical aspect of various scientific and industrial fields, where the properties of molecules hold significant importance. In this study, a 3-fold methodology design is presented that leverages the power of generative artificial intelligence (AI), predictive modeling, and reinforcement learning to create tailored molecules with desired properties. This model synergistically combines deep learning techniques with Self-Referencing Embedded Strings (SELFIES) molecular representation to build a generative model that generates valid molecules and a graphical neural network model that accurately forecasts molecular properties. The Variational Autoencoder (VAE) coupled with reinforcement learning helps refine molecule generation based on targeted attributes. …

“Everything Is Not What It Seems”: Discovering Public Relations In Business Sectors In Vietnam, Ba-Anh-Tu Truong

LSU Master's Theses

Vietnam is a shining star in emerging markets, with an annual economic growth rate of 6-7%. Its abundant labor supply, stable political climate, and geographic proximity to major global supply chains make it an ideal candidate for manufacturer planning in advanced economies such as the United States, Europe, Australia, Japan, China, and Korea. Investing in public relations and communication management, especially understanding the Vietnamese market, effectively prepares multinational corporations for future business expansion. This mission is difficult for academia and industrial professionals since Vietnam is a “silent country” in public relations research. To fill the gap, this thesis employs the …

Seeking A Uniform Approach To Trumpet Sound Concept, Coleman T. Scott

LSU Doctoral Dissertations

One of the most challenging aspects of trumpet playing that pedagogues must teach is how to produce a professional tone. Trumpet students are often good musicians, play correct notes and rhythms, and even have proficient technique. However, many are unable to consistently produce a tone comparable to that of a professional. There are a number of methods that can be utilized to improve a student’s tone quality. In my own teaching and in the teaching of many professionals, tone concept has been used as a catalyst for the improvement of tone quality. A students’ exposure to a professional-quality trumpet tone …

Post-Modeling Adjustments And Delivered Dose Verification Of The 6fff Beam Model Commissioned For The Monaco Treatment Planning System, Grant C. Debevec

LSU Master's Theses

External beam radiation therapy has been shown to be an effective treatment method for tumors and abnormalities of the spine and vertebral region. Treating the spine using a stereotactic body radiation therapy (SBRT) technique can reduce toxicity to the spinal cord. The 6 MV flattening filter free (6FFF) beam model is currently used to plan and calculate dose for SBRT treatment plans, and the treatment plans are delivered using a linear accelerator (LINAC).

The commissioned beam model represents an invariant component of a LINAC. For volumetric modulated arc therapy (VMAT) treatment plans, the multileaf collimator (MLC) positions are changing throughout …

The Effects Of Global Temperature Change On Radon Exposure In Permafrost Areas, Melaan Bender

LSU Master's Theses

Global temperature increase leads to thawing of permafrost that protects local populations from radiation exposure by blocking radon-222 migrating into dwellings. This study aims at assessing the effects of permafrost thawing on the concentrations of radon in indoor air. An approach is developed to predict the evolution of the ²²²Rn concentrations by various processes including radon exhalation from thawed soil. It is found that as frozen soil thaws, more radon can enter dwellings, increasing the concentration of ²²²Rn in indoor air and elevating radiation-induced health risks. This study provides useful insight that will enhance understanding of potential climate …

Race And Racism Through The Lens Of Black Men: Exploring The Talk And Lessons Black Fathers Teach Their Children About Race And Racism In America., Conial C. Caldwell Jr

LSU Doctoral Dissertations

The history of racism and racial violence is embedded in the fabric of America and has driven Black people to develop strategies to navigate society that racism has produced. “The Talk” is a racial socialization practice and cultural phenomenon that has existed within Black families for centuries and remains a necessity due to the ongoing acts of racism and violence of Black Americans. Prior research has found that parents rear their children based on their own lived experiences, which many Black Americans have experienced or have been exposed to racism and racial violence. Yet, there are few empirical studies that …

Mind The Time: A Synthesis Of Pedagogical Approaches For Improving Rhythm And Meter For Instrumentalists As Applied To Bassoon, Annalea Milligan

LSU Doctoral Dissertations

Rhythm is imperative for the performing musician. This project aims to provide bassoonists with a resource for a rhythmic or metric-focused approach to their applied study. Pedagogical resources for improving rhythm in music through the lens of four categories are identified: experiential, pattern and phrasing, mapping, and repetition. Exercises created from a synthesis of each pedagogical approach demonstrate possible application to bassoon literature by exploring the ways in which an intentional awareness of rhythm can improve technical playing and build practice and performance confidence. By emphasizing the importance of rhythm, its components, and the various approaches to teaching and understanding …

The Theory Of X-Ray And Neutron Interferometry Using A Modulated Phase Grating, Hunter C. Meyer

LSU Master's Theses

Grating interferometry exploits wave interference to simultaneously image multiple physical properties of an object and can be performed using X-rays or neutrons. The modulated phase grating interferometer (MPGI) is a recently developed interferometry system that allows for the simultaneous acquisition of attenuation, differential-phase contrast, and dark-field images, which highlight X-ray or neutron absorption, refraction, and small angle scatter, respectively. This interferometry system uses a single grating, with a relatively high period envelope function, to produce interference fringes that can be directly resolved by standard high resolution detectors. The grating is sampled at a relatively low pitch, to meet coherence requirements …

Estimating Crustal Thickness In Northwest Louisiana Using The Receiver Function Method, Delton Samuel

LSU Master's Theses

I aim to constrain the crustal thickness of the Sabine Block in the Sabine Uplift region of northwest Louisiana, using the frequency domain receiver function deconvolution technique followed by H-κ stacking. The passive margin on the southern edge of the North American continent experienced an active tectonic history, including the spreading events that led to the formation of the Gulf of Mexico. A previous study proposed the Sabine Block is a residual fragment of Proterozoic orogenic origin; however, its full extent and geometry are up for debate. It is now overlain by thick sedimentary sequences ranging from ~4-6 km deposited …