Digital Commons Network^™

Effects Of Caloric Restriction On Human Physiological, Psychological, And Behavioral Outcomes: Highlights From Calerie Phase 2, James L. Dorling, Stephan Van Vliet, Kim M. Huffman, William E. Kraus, Manjushri Bhapkar, Carl F. Pieper, Tiffany Stewart, Sai Krupa Das, Susan B. Racette, Susan B. Roberts, Eric Ravussin, Leanne M. Redman, Corby K. Martin

Clinical Research Faculty Publications

Caloric restriction (CR) is a strategy that attenuates aging in multiple nonhuman species. The Comprehensive Assessment of Long-term Effects of Reducing Intake of Energy (CALERIE) trials are part of a research program aiming to test the effects of CR on aging and longevity biomarkers in humans. Building on CALERIE phase 1, CALERIE phase 2 (CALERIE 2) was the largest study to date to assess sustained CR in healthy humans without obesity. In a 24-month randomized controlled trial comprising 218 participants at baseline, CALERIE 2 showed that moderate CR, 11.9% on average, induced improvements in aging-related biomarkers without adversely affecting psychological …

Adipose Depot-Specific Effects Of 16 Weeks Of Pioglitazone On In Vivo Adipogenesis In Women With Obesity: A Randomised Controlled Trial, Ursula White, Mark D. Fitch, Robbie A. Beyl, Marc K. Hellerstein, Eric Ravussin

Clinical Research Faculty Publications

Aims/hypothesis: In vitro and rodent studies suggest that pioglitazone, a thiazolidinedione, can promote adipogenesis in adipose tissue (AT); however, there is a lack of in vivo studies in humans to support these findings. The objectives of this randomised, placebo-controlled, parallel-arm trial were to test if pioglitazone stimulates in vivo adipogenesis in the subcutaneous adipose tissue depots and if these measures were related to metabolic health outcomes in women with obesity. Methods: Forty-one healthy women with obesity (20 black; 21 white; 29 ± 6 years; BMI 32.0 ± 1.7 kg/m2; 44.0 ± 3.6% body fat) were randomised to consume 30 mg/day …

Effect Of An Office-Based Intervention On Visceral Adipose Tissue: The Workactive-P Randomized Controlled Trial, James L. Dorling, Christoph Höchsmann, Catrine Tudor-Locke, Robbie Beyl, Corby K. Martin

Clinical Research Faculty Publications

Office-based activity reduces sedentariness, yet no randomized controlled trials (RCTs) have assessed how such activity influences visceral adipose tissue (VAT). This study examined the effect of an office-based, multicomponent activity intervention on VAT. The WorkACTIVE-P RCT enrolled sedentary office workers (body mass index: 31.4 (standard deviation (SD) 4.4) kg/m2 ) to an intervention (n = 20) or control (n = 20) group. For 3 months, the intervention group received an office-based pedal desk, further to an intervention promoting its use and increased walking. The control group maintained habitual activity. At baseline and follow-up, VAT, cardiometabolic disease risk markers, physical activity, …

Leucine And Sildenafil Combination Therapy Reduces Body Weight And Metformin Enhances The Effect At Low Dose: A Randomized Controlled Trial, Candida J. Rebello, Michael B. Zemel, Orville Kolterman, Gilbert Alexander Fleming, Frank L. Greenway

Clinical Research Faculty Publications

Background:This study evaluated the potential of activating the fuel-sensing enzymes Adenine monophosphate (AMP)-activated protein kinase and the deacetylase sirtuin1, to promote weight loss. We tested the efficacy of a fixed dose combination of the amino acid leucine and 2 well-characterized agents with established safety profiles to modulate energy metabolism and facilitate weight loss.Study Question:Will a combination of l-leucine with low-dose metformin and sildenafil produce a novel synergistic interaction that reduces body weight?Study Design:We conducted a 24-week randomized controlled trial evaluating the effect on weight loss of leucine 1.1 g and sildenafil 1.0 mg or 4.0 mg, with and without metformin …

Agronomic And Environmental Performance Of Biochar Amendment In Alluvial Soils Under Subtropical Sugarcane Production, Negar Tafti, Jim Wang, Lewis Gaston, Jong Hwan Park, Meng Wang, Scott Pensky

Faculty Publications

This study investigated the amendment of sugarcane bagasse biochar (SCBB) on soil fertility, crop yield, and nutrient loss in two different-textured soils under sugarcane (Saccharum spp.) production. Eleven megagrams per hectare of freshly incorporated biochar increased yield of plant cane by 22% in light-texture soil (LS) and 12% in heavy-textured soil (HS). Although the overall yield of the ratoon crop was lower, the biochar treatment produced 20 and 14% higher yields than the control at the LS and HS sites, respectively. Biochar increased soil carbon (Csoil) across LS and HS sites by 15% and decreased the soil C/N ratio by …

Maternal Gdm Status, Genetically Determined Blood Glucose, And Offspring Obesity Risk: An Observational Study, Qiying Song, Leishen Wang, Huikun Liu, Zhaoxia Liang, Yuhang Chen, Dianjianyi Sun, Weiqin Li, Junhong Leng, Xilin Yang, Marly Augusto Cardoso, Gang Hu, Lu Qi

Population and Public Health Science Faculty Publications

Objective: The purpose of this study was to estimate the associations of genetically determined maternal blood glucose levels with obesity-related outcomes among children from pregnancies with and without gestational diabetes mellitus (GDM). Methods: A total of 1,114 mothers with (N = 560) and without (N = 554) GDM and their children were included in the present study. A maternal genetic risk score (GRS) for blood glucose was constructed on the basis of 17 single-nucleotide polymorphisms identified from a recent genome-wide association study. Results: It was found that maternal GRS for blood glucose showed different associations with offspring risk of overweight …

Effects Of Lifestyle Intervention Of Maternal Gestational Diabetes Mellitus On Offspring Growth Pattern Before Two Years Of Age, Jing Li, Jinnan Liu, Cuiping Zhang, Gongshu Liu, Junhong Leng, Leishen Wang, Weiqin Li, Zhijie Yu, Gang Hu, Juliana C.N. Chan, Xilin Yang

Population and Public Health Science Faculty Publications

No abstract provided.

Global Health Risk Factors: Physical Inactivity, Fiona Bull, Regina Guthold, Daniel J. Friedman, Peter Katzmarzyk

Population and Public Health Science Faculty Publications

Regular physical activity improves physical and mental health across all ages, and provides many social, environmental, and economic co-benefits. Physical activity includes all forms of active recreation, sports participation, cycling and walking for transportation, as well as activity undertaken at home or for work. Despite well-established benefits, one quarter of adults and three quarters of adolescents are not sufficiently active. The policy and practice of promoting physical activity integrates multidisciplinary knowledge from across clinical and social medicine, exercise science, health psychology, public health, transport, and environmental studies, to improve global health. With increasing recognition of its role in promoting better …

Association Between The Consumption Of Breakfast And The Recommendation Of Physical Activity And The Nutritional Status In Children, Eduardo Rossato De Victo, Gerson Ferrari, Dirceu Solé, Carlos André Miranda Pires, Timóteo Leandro Araújo, Peter T. Katzmarzyk, Victor Keihan Rodrigues Matsudo

Population and Public Health Science Faculty Publications

The scope of this article is to associate breakfast consumption (BC) with moderate to vi-gorous physical activity (MVPA) intensity and the nutritional status of children aged 9-11. The sample consisted of 432 children from São Caetano do Sul participating in the International Study of Childhood Obesity Lifestyle and the Environment. Data were collected between 2012 and 2013. The weekly BC was obtained using the self-reported method. To measure MVPA, children used ac-celerometers and were classified into two groups (< 60 versus ≥60 min/day). The nutritional status was presented by body mass index (BMI). Logistic regression models were used, adjusted for gender, age, race, parental educational level and eating score. The mean BC was 5.25 (CI95%: 5.1-5.4) days/week and the MVPA was 59.29 (CI95%: 57.3-61.7) min/day. In the total sample, 55.8% of the children did not perform ≥60 min/day of MVPA and 50.2% were overweight or obese. The daily BC was not associated with MVPA, however, it reduced the odds of children being overweight or obese (OR: 0.51; CI95%: 0.34-0.76; p=0.001). Daily BC reduced the odds of excess weight or obesity in children, though it did not increase the chances of children meeting the recommendations of MVPA. Public policies should encourage daily BC to prevent excess weight or obesity.

Nutritional Status, Physical Activity, Sedentary Behavior, Diet, And Lifestyle In Childhood: An Analysis Of Respiratory Diseases In Adolescence, Eduardo Rossato De Victo, Gerson Ferrari, Victor Keihan Rodrigues Matsudo, Carlos André Miranda Pires, Timóteo Leandro Araújo, Peter Todd Katzmarzyk, Dirceu Solé

Population and Public Health Science Faculty Publications

Objective: To evaluate the association between nutritional status, physical activity, sedentary behavior, diet, and lifestyle in childhood with respiratory diseases during adolescence. Methods: Prospective study conducted in Saõ Caetano do Sul, Saõ Paulo-Brazil, as part of the International Study of Childhood Obesity, Lifestyle and the Environment (ISCOLE). During childhood, indicators of lifestyle (body composition, physical activity, sedentary behavior, diet) and family environment were measured in 2012 and 2013. After five years, participants answered the International Study of Asthma and Allergies in Childhood (ISAAC) questionnaire for the diagnosis of respiratory diseases (asthma and/or rhinitis). Analyses were determined by logistic regression. Results: …

Bioenergetics Of Islet Preparations In A Pilot Clinical Trial Of Peri-Transplant Hydroxychloroquine For Autologous Islet Transplantation, Ruth E. Mcdowell, Khawla F. Ali, Saloni Lad, Vicente T. San Martin, Rita Bottino, Matthew Walsh, Tyler Stevens, William Wilke, John P. Kirwan, Betul Hatipoglu

Division of Basic Science Faculty Publications

The inflammatory response is an obstacle to success in both allogeneic and autologous islet transplantation. In autologous islet transplantation (AIT), however, the recipient is also the donor, permitting pretreatment of donor/recipient for a controlled duration prior to transplantation. We sought to exploit this feature of (AIT) by pretreating donor/recipients with chronic pancreatitis undergoing total pancreatectomy and autologous islet transplantation (TPAIT) to test the hypothesis that peri-transplant treatment with the FDA-approved anti-inflammatory hydroxychloroquine (HCQ) improves graft function. In this randomized placebo-controlled pilot clinical study, patients (n = 6) were treated with oral HCQ for 30 days prior to and 90 days …

Digital Tools To Support Family-Based Weight Management For Children: Mixed Methods Pilot And Feasibility Study, Amanda E. Staiano, Jenelle R. Shanley, Holly Kihm, Keely R. Hawkins, Shannon Self-Brown, Christoph Höchsmann, Melissa C. Osborne, Monique M. Leblanc, John W. Apolzan, Corby K. Martin

Clinical Research Faculty Publications

Background: Family-based behavioral therapy is an efficacious approach to deliver weight management counseling to children and their parents. However, most families do not have access to in-person, evidence-based treatment. We previously developed and tested DRIVE (Developing Relationships that Include Values of Eating and Exercise), a home-based parent training program to maintain body weight among children at risk for obesity, with the intent to eventually disseminate it nationally alongside SafeCare, a parent support program that focuses on parent-child interactions. Currently the DRIVE program has only been tested independently of SafeCare. This study created the “mHealth DRIVE” program by further adapting DRIVE …

Beyond Weight Loss: Current Perspectives On The Impact Of Calorie Restriction On Healthspan And Lifespan, Maryam Kebbe, Joshua R. Sparks, Emily W. Flanagan, Leanne M. Redman

Clinical Research Faculty Publications

Introduction: Changes to mental, physical, and physiological functions drive the complex processes underlying the variable progression of human aging. Nutritional interventions are one of the most promising non-pharmacological therapeutics to attenuate aging in humans. This narrative review aims to describe the implications of moderate and prolonged calorie restriction (CR) in healthy adults without obesity that occur beyond weight loss. Areas covered: Findings from CR studies, such as the CALERIE (Comprehensive Assessment of Long-term Effects of Reducing Intake of Energy) trials, the most rigorous trials to date examining a prolonged 25% CR, are described. The main areas covered include; changes to …

Constrained Learning Of Task-Related And Spatially-Coherent Dictionaries From Task Fmri Data, Sreekrishna Ramakrishnapillai, Harris R. Lieberman, Jennifer C. Rood, Stefan M. Pasiakos, Kori Murray, Preetham Shankapal, Owen T. Carmichael

Clinical Research Faculty Publications

Dictionary learning and sparse coding techniques overcome limitations of traditional voxel-level analyses of task-based functional magnetic resonance imaging (fMRI) data by identifying broader temporal and spatial patterns of brain activity. However, prior applications of these methods to task-related fMRI data are not simultaneously optimized to find temporal patterns of activity that change in concert with changes in task conditions and spatial patterns that leverage existing neuroscience knowledge. In this study we present a new sparse dictionary learning method that uses prior knowledge of the temporal pattern of task conditions and the locations of brain regions hypothesized to be involved in …

Eat Clean And Safe Food: A Food-Based Dietary Guideline For The Elderly In South Africa, Makenzie Miller, Wilna Oldewage-Theron, Carin Napier

Faculty Publications

As the population of elderly individuals in South Africa (SA) grows, there is a need to promote the continued health of these persons as they progress through the life cycle. Food Based Dietary Guidelines (FBDGs) for the SA elderly were developed to address this need. These thirteen guidelines for the elderly collectively offer a basis of health practices that the elderly can follow to ensure that they are taking the right steps toward maintaining their health. While the guideline ‘Eat clean and safe food’ is not included in the current SA FBDGs, this recommendation is of particular importance to the …

Food And Beverages Promoting Elderly Health: Six Food-Based Dietary Guidelines To Plan Good Mixed Meals For Elderly South Africans, Sanjoy Saha, Upasana Mukherjee, Makenzie Miller, Li Ling Peng, Carin Napier, Heleen Grobbelaar, Wilna Oldewage-Theron

Faculty Publications

Studies have shown that the elderly are at a higher risk of developing malnutrition due to physiological and pathological changes. Several studies have confirmed that older South Africans have insufficient dietary diversity, resulting in nutritional deficiencies. Furthermore, poor and uninformed dietary choices are associated with the development of several diseases and increased mortality. Following the Elderly Food Based Dietary Guidelines (EFBDGs) related to promoting elderly health could ensure that the elderly have an adequate intake of nutritious foods. This paper provides an overview of six FBDGs relating to promoting health and is based on the South African Food Based Dietary …

Effects Of Immersion In Fermented Tea Liquid And Steam Treatments On Physicochemical Properties And Ginsenoside Profiles Of Korean Ginseng, Peerarat Doungtip, Kyung Tack Kim, Hee Do Hong, Shin Eun Ju, Jae Woong Choi, Thanyaporn Siriwoharn, Witoon Prinyawiwatkul, Sujinda Sriwattana

Faculty Publications

The study was performed to investigate some changes in physicochemical characteristics and ginsenoside profiles of Korean ginseng after being subjected to immersion in fermented tea liquid (FTL) [at a ratio of ginseng powder: FTL; 1:4, 1:6, and 1:8, w/w] and steam treatments (with and/or without steam). The ratio at 1:4 w/w showed the highest yield of bioactive components and was, therefore, selected to evaluate the effects of steam conditions [temperatures (60, 80, and 100°C) and times (1, 2, and 3 hr)]. The sample treated with FTL, regardless of the steam treatment, had darker color and higher antioxidant activity (IC50 for …

Comparison Of Abdominal Visceral Adipose Tissue Measurements In Adolescents Between Magnetic Resonance Imaging And Dual-Energy X-Ray Absorptiometry, Chelsea L. Kracht, Peter T. Katzmarzyk, Amanda E. Staiano

Population and Public Health Science Faculty Publications

Excess abdominal visceral adipose tissue (VAT) is associated with cardiometabolic risk factors in adolescents. VAT is mainly measured using Magnetic Resonance Imaging (MRI), yet dual-energy x-ray absorptiometry (DXA) is more affordable and available. The purpose was to compare adolescent VAT values obtained by MRI and DXA. A sample of 330 adolescents 10–16 years of age were recruited (52.3% female, 58.5% White). Abdominal VAT was measured using a General Electric (GE) Discovery MRI scanner with imaging software. A whole-body DXA (GE iDXA) scan was performed, and software calculated VAT within the android region. Wilcoxon signed-rank t-tests were used to determine differences …

Perceptions Of A Pragmatic Family-Centered Approach To Childhood Obesity Treatment, Betty M. Kennedy, Genevieve Davison, Lauren A. Fowler, Erika Rodriguez-Guzman, Myra L. Collins, Alison Baker, Stephen Cook, Jeanne Lindros, Denise E. Wilfley, Ava J. Zebrick, Amanda E. Staiano

Population and Public Health Science Faculty Publications

Background: Few studies have examined both parent and child preferences regarding family-based weight management programs (WMPs) delivered in primary care settings, especially among racial minority populations. The purpose of this study was to determine the perceptions that parents and their children/adolescents have about the components that should be included in a family-based WMP and to identify perceived preferences, benefits, and/or barriers to participation. Methods: A sample of 60 participants (30 parents and 30 children/adolescents) participated in 1 of 5 separate structured focus groups, using probing questions and the nominal group technique (NGT). Parents reported demographics for themselves and their children/adolescents. …

Effect Of Hydrological Fluctuation On Nutrient Stoichiometry And Trade-Offs Of Carex Schmidtii, Dongjie Zhang, Qing Qi, Shouzheng Tong, Jim Wang, Mingye Zhang, Guanglei Zhu, Xianguo Lu

Faculty Publications

Nutrient stoichiometry and trade-offs focusing on the mass balance of multiple nutrient elements play important roles in the response mechanism of plants to environmental stresses in wetlands. Nonetheless, little is known about the response dynamics and trade-offs of nutrient elements of wetland plants, especially potassium (K), under hydrological fluctuation conditions. Greenhouse experiments have been undertaken to assess the effects of initial water level (DW), water-level amplitude (AW), lasted time and their interactions on the response processes of nutrient stoichiometry of Carex schmidtii. Plant nutrient trade-offs and limitation were investigated under multiple hydrological fluctuation conditions. Results showed that plant nitrogen (N), …

Attenuated Early Pregnancy Weight Gain By Prenatal Lifestyle Interventions Does Not Prevent Gestational Diabetes In The Life-Moms Consortium, Leanne M. Redman, Kimberly L. Drews, Samuel Klein, Linda Van Horn, Rena R. Wing, Xavier Pi-Sunyer, Mary Evans, Kaumudi Joshipura, S. Sonia Arteaga, Alison G. Cahill, Rebecca G. Clifton, Kimberly A. Couch, Paul W. Franks, Dympna Gallagher, Debra Haire-Joshu, Corby K. Martin, Alan M. Peaceman, Suzanne Phelan, Elizabeth A. Thom, Susan Z. Yanovski, William C. Knowler

Clinical Research Faculty Publications

Aims: To examine the effect of lifestyle (diet and physical activity) interventions on the prevalence of GDM, considering the method of GDM ascertainment and its association with early pregnancy characteristics and maternal and neonatal outcomes in the LIFE-Moms consortium. Methods: LIFE-Moms evaluated the effects of lifestyle interventions to optimize gestational weight gain in 1148 pregnant women with BMI ≥ 25 kg/m2 and without known diabetes at enrollment, compared with standard care. GDM was assessed between 24 and 31-weeks gestation by a 2-hour, 75-gram OGTT or by local clinical practice standards. Results: Lifestyle interventions initiated prior to 16 weeks reduced early …

Association Of Baseline And Longitudinal Changes In Body Composition Measures With Risk Of Heart Failure And Myocardial Infarction In Type 2 Diabetes: Findings From The Look Ahead Trial, Kershaw V. Patel, Judy L. Bahnson, Sarah A. Gaussoin, Karen C. Johnson, Xavier Pi-Sunyer, Ursula White, Kayloni L. Olson, Alain G. Bertoni, Dalane W. Kitzman, Jarett D. Berry, Ambarish Pandey

Clinical Research Faculty Publications

Background: Intentional weight loss is associated with lower risk of heart failure (HF) and atherosclerotic cardiovascular disease among patients with type 2 diabetes. However, the contribution of baseline measures and longitudinal changes in fat mass (FM), lean mass (LM), and waist circumference (WC) to the risk of HF and myocardial infarction (MI) in type 2 diabetes is not well established. Methods: Adults from the Look AHEAD trial (Action for Health in Diabetes) without prevalent HF were included. FM and LM were predicted using validated equations and compared with dual-energy x-ray absorptiometry measurements in a subgroup. Adjusted Cox models were used …

Perceived Vulnerability To Covid-19 Infection From Event Attendance: Results From Louisiana, Usa, Two Weeks Preceding The National Emergency Declaration, Ran Li, Bingcheng Yang, Jerrod Penn, Bailey Houghtaling, Juan Chen, Witoon Prinyawiwatkul, Brian E. Roe, Danyi Qi

Faculty Publications

Background: Individual perceptions of personal and national threats posed by COVID-19 shaped initial response to the pandemic. The aim of this study was to investigate the changes in residents' awareness about COVID-19 and to characterize those who were more aware and responsive during the early stages of the pandemic in Louisiana.

Methods: In response to the mounting threat of COVID-19, we added questions to an ongoing food preference study held at Louisiana State University from March 3rd through March 12th, 2020. We asked how likely it was that the spread of the coronavirus will cause a national public health crisis …

Calorie Restriction For Enhanced Longevity: The Role Of Novel Dietary Strategies In The Present Obesogenic Environment, James L. Dorling, Corby K. Martin, Leanne M. Redman

Clinical Research Faculty Publications

Calorie restriction (CR) is a potent modulator of longevity in multiple species. A growing body of evidence shows that sustained periods of CR without malnutrition improves risk factors involved in the pathophysiology of type 2 diabetes, cardiovascular diseases, cancer, and neurological disorders in humans. Innovative dietary strategies such as intermittent fasting and protein restriction have recently emerged as alternative approaches that improve markers of aging. Some of these newer strategies might provide benefits for healthy aging with little to no CR and therefore, compared to traditional CR, may be easier to follow. Further to providing an update of CR studies …

Qcm Sensor Arrays, Electroanalytical Techniques And Nir Spectroscopy Coupled To Multivariate Analysis For Quality Assessment Of Food Products, Raw Materials, Ingredients And Foodborne Pathogen Detection: Challenges And Breakthroughs†, David K. Bwambok, Noureen Siraj, Samantha Macchi, Nathaniel E. Larm, Gary A. Baker, Rocío L. Pérez, Caitlan E. Ayala, Charuksha Walgama, David Pollard, Jason D. Rodriguez, Souvik Banerjee, Brianda Elzey, Isiah M. Warner, Sayo O. Fakayode

Faculty Publications

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Quality checks, assessments, and the assurance of food products, raw materials, and food ingredients is critically important to ensure the safeguard of foods of high quality for safety and public health. Nevertheless, quality checks, assessments, and the assurance of food products along distribution and supply chains is impacted by various challenges. For instance, the development of portable, sensitive, low-cost, and robust instrumentation that is capable of real-time, accurate, and sensitive analysis, quality checks, assessments, and the assurance of food products in the field and/or in the production line in a food …

Mobilization Of Iron Stored In Bacterioferritin Is Required For Metabolic Homeostasis In Pseudomonas Aeruginosa, Achala N.D. Punchi Hewage, Leo Fontenot, Jessie Guidry, Thomas Weldeghiorghis, Anil K. Mehta, Fabrizio Donnarumma, Mario Rivera

Faculty Publications

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Iron homeostasis offers a significant bacterial vulnerability because pathogens obtain essential iron from their mammalian hosts, but host-defenses maintain vanishingly low levels of free iron. Although pathogens have evolved mechanisms to procure host-iron, these depend on well-regulated iron homeostasis. To disrupt iron homeostasis, our work has targeted iron mobilization from the iron storage protein bacterioferritin (BfrB) by blocking a required interaction with its cognate ferredoxin partner (Bfd). The blockade of the BfrB–Bfd complex by deletion of the bfd gene (∆bfd) causes iron to irreversibly accumulate in BfrB. In this study we …

Development Of A Degree-Day Model To Predict Egg Hatch Of Aedes Albopictus, Kristen B. Healy, Emily Dugas, Dina M. Fonseca

Faculty Publications

Aedes albopictus, the Asian tiger mosquito, is an important nuisance mosquito species and known vector of arboviruses such as dengue, chikungunya, and Zika. Despite their cosmopolitan distribution around the world, there is a paucity of accurate predictive models based on rates of development at different temperatures (degree-day models). These types of models can benefit mosquito control districts by predicting when to target early-season larval development, when populations are likely at their lowest levels. In this study, we determined the effect of temperature and nutrient levels on the development rates and male and female adult size of 2 Ae. albopictus populations: …

17Β-Estradiol Promotes Acute Refeeding In Hungry Mice Via Membrane-Initiated Erα Signaling, Kaifan Yu, Yanlin He, Ilirjana Hyseni, Zhou Pei, Yongjie Yang, Pingwen Xu, Xing Cai, Hesong Liu, Na Qu, Hailan Liu, Yang He, Meng Yu, Chen Liang, Tingting Yang, Julia Wang, Pierre Gourdy, Jean Francois Arnal, Francoise Lenfant, Yong Xu, Chunmei Wang

Division of Basic Science Faculty Publications

Objective: Estrogen protects animals from obesity through estrogen receptor α (ERα), partially by inhibiting overeating in animals fed ad libitum. However, the effects of estrogen on feeding behavior in hungry animals remain unclear. In this study, we examined the roles of 17β-estradiol (E2) and ERα in the regulation of feeding in hungry female animals and explored the underlying mechanisms. Methods: Wild-type female mice with surgical depletion of endogenous estrogens were used to examine the effects of E2 supplementation on acute refeeding behavior after starvation. ERα-C451A mutant mice deficient in membrane-bound ERα activity and ERα-AF20 mutant mice lacking ERα transcriptional activity …

Estrogen Receptor-Α Expressing Neurons In The Ventrolateral Vmh Regulate Glucose Balance, Yanlin He, Pingwen Xu, Chunmei Wang, Yan Xia, Meng Yu, Yongjie Yang, Kaifan Yu, Xing Cai, Na Qu, Kenji Saito, Julia Wang, Ilirjana Hyseni, Matthew Robertson, Badrajee Piyarathna, Min Gao, Sohaib A. Khan, Feng Liu, Rui Chen, Cristian Coarfa, Zhongming Zhao, Qingchun Tong, Zheng Sun, Yong Xu

Division of Basic Science Faculty Publications

Brain glucose-sensing neurons detect glucose fluctuations and prevent severe hypoglycemia, but mechanisms mediating functions of these glucose-sensing neurons are unclear. Here we report that estrogen receptor-α (ERα)-expressing neurons in the ventrolateral subdivision of the ventromedial hypothalamic nucleus (vlVMH) can sense glucose fluctuations, being glucose-inhibited neurons (GI-ERαvlVMH) or glucose-excited neurons (GE-ERαvlVMH). Hypoglycemia activates GI-ERαvlVMH neurons via the anoctamin 4 channel, and inhibits GE-ERαvlVMH neurons through opening the ATP-sensitive potassium channel. Further, we show that GI-ERαvlVMH neurons preferentially project to the medioposterior arcuate nucleus of the hypothalamus (mpARH) and GE-ERαvlVMH neurons preferentially project to the dorsal Raphe nuclei (DRN). Activation of ERαvlVMH …

Estrogen-Sensitive Medial Preoptic Area Neurons Coordinate Torpor In Mice, Zhi Zhang, Fernando M.C.V. Reis, Yanlin He, Jae W. Park, Johnathon R. Divittorio, Nilla Sivakumar, J. Edward Van Veen, Sandra Maesta-Pereira, Michael Shum, India Nichols, Megan G. Massa, Shawn Anderson, Ketema Paul, Marc Liesa, Olujimi A. Ajijola, Yong Xu, Avishek Adhikari, Stephanie M. Correa

Division of Basic Science Faculty Publications

Homeotherms maintain a stable internal body temperature despite changing environments. During energy deficiency, some species can cease to defend their body temperature and enter a hypothermic and hypometabolic state known as torpor. Recent advances have revealed the medial preoptic area (MPA) as a key site for the regulation of torpor in mice. The MPA is estrogen-sensitive and estrogens also have potent effects on both temperature and metabolism. Here, we demonstrate that estrogen-sensitive neurons in the MPA can coordinate hypothermia and hypometabolism in mice. Selectively activating estrogen-sensitive MPA neurons was sufficient to drive a coordinated depression of metabolic rate and body …