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Molecular Approaches To Detect And Control Cercospora Kikuchii In Soybeans, Ashok Kumar Chanda Jan 2012

Molecular Approaches To Detect And Control Cercospora Kikuchii In Soybeans, Ashok Kumar Chanda

LSU Doctoral Dissertations

Cercospora leaf blight (CLB) caused by Cercospora kikuchii, has become a troublesome disease in the southern United States. C. kikuchii produces a non-hostspecific phytotoxin and a pathogenicity factor known as cercosporin during infection of soybean. A quantitative real-time PCR assay was developed for detection and quantification of C. kikuchii. The sensitivity of detection is 1 pg of genomic DNA. The assay detected the presence of C. kikuchii in soybean leaves long before the appearance of disease symptoms. C. kikuchii DNA levels in soybean leaves increased slowly during early soybean development, followed by a quick increase at late reproductive stages. Results …

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Canning Foods And Selling Modernity: The Canned Food Industry And Consumer Culture, 1898-1945, Kristi Renee Whitfield Jan 2012

Canning Foods And Selling Modernity: The Canned Food Industry And Consumer Culture, 1898-1945, Kristi Renee Whitfield

LSU Doctoral Dissertations

At the turn of the twentieth century, Americans feared commercially canned foods. From the Spanish American War until well into the 1920’s, canned foods received a barrage of media attacks and accusations of unhealthiness, lack of cleanliness, and a lack of transparency and regulation in processing. Moreover, as gastrointestinal distress was quite prevalent among American society, many Americans feared that it was commercial foods that were making them sick. By the time Americans were coming home from World War II, the climate of opinion concerning commercially canned foods had changed, and this was in large part due to the unyielding …

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Defense Gland Protein From Coptotermes Formosanus, Ioan-Horia Negulescu Jan 2012

Defense Gland Protein From Coptotermes Formosanus, Ioan-Horia Negulescu

LSU Doctoral Dissertations

Coptotermes formosanus Shiraki is the most invasive termite species and has the largest economic impact of all the subterranean termites in the US. Although a lot of work has been done on the determination of chemical composition of frontal gland secretion from different families of termites, the protein component of those secretions was relatively overlooked. This study started with the observation that proteins are present in the frontal gland secretion of Coptotermes formosanus Shiraki soldiers. The goal was to characterize one of these proteins by identifying its DNA sequence which would lead to protein sequence, function, structure and role it …

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An Exploratory Study Of Informal Science Learning By Children Ages 2-12 Years At Selected U.S. Children's Gardens, Mary Flake Legoria Jan 2012

An Exploratory Study Of Informal Science Learning By Children Ages 2-12 Years At Selected U.S. Children's Gardens, Mary Flake Legoria

LSU Doctoral Dissertations

ABSTRACT This exploratory study was conducted at four children’s gardens in major botanical gardens across the United States to determine if children became more aware and knowledgeable of plants while visiting these gardens. This was determined through the children’s garden stakeholders’ perspectives; the stakeholders of this study were the children and parents who visited the gardens. Their views were acquired through on-site observations and interviews. The purposive sample comprised 64 participants including 40 children (19 girls and 21 boys, ages 2- 12 years). There were 18 mothers, 3 fathers, 3 grandmothers, and 1 grandfather. The 40 children were observed and …

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Forage Systems For Finishing Steers In South Louisiana, Jose M. Rodriguez Jan 2012

Forage Systems For Finishing Steers In South Louisiana, Jose M. Rodriguez

LSU Master's Theses

Research has found conflicting results on animal performance and carcass traits associated with the use of forage as the primary feed source for finishing cattle. Consumer interest in forage-fed products has grown and little research has been done comparing performance of forage-fed animals and beef finished on different forages. Spring weaned calves (n=54; 257 ± 2.5 kg; 3/8 Gelbvieh, 3/8 Red Angus, and 1/4 Brahman) were used in the evaluation of three forage systems (S1, S2, and S3) on a 100% forage diet in two consecutive years (June 2009 and 2010). Steers were divided into 9 groups based on initial …

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Investigating The Role Of Genetic Variation In Long Run Economic Outcomes, Charles Justin Cook Jan 2012

Investigating The Role Of Genetic Variation In Long Run Economic Outcomes, Charles Justin Cook

LSU Doctoral Dissertations

This dissertation empirically tests whether adaptations resulting from the Neolithic Revolution, or the widespread adoption of sedentary agriculture for sustenance, have led to economic differences. The development of sedentary agriculture constituted an environmen- tal shift from the previous sustenance method of hunting and gathering. This environmental shift resulted in the natural selection of certain traits. I seek to exploit differences in these traits to measure differing economic outcomes. Two main adaptations are considered in this work: the ability to consume milk, or lactose tolerance, and resistance to infectious Eurasian diseases, which is the result of genetic variation. The first essay …

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The Effect Of Frozen Storage On The Survival Of Probiotic Microorganisms Found In Traditional And Commercial Kefir, Keely Virginia O'Brien Jan 2012

The Effect Of Frozen Storage On The Survival Of Probiotic Microorganisms Found In Traditional And Commercial Kefir, Keely Virginia O'Brien

LSU Master's Theses

Kefir is a fermented milk traditionally made from a unique starter culture, which consists of numerous bacteria and yeast species bound together in an exopolysaccharide matrix produced by certain lactic acid bacteria. Many health benefits are associated with traditionally produced kefir; however, bulging and leaking packaging, caused by secondary yeast fermentation during storage, has limited large scale manufacture traditionally produced kefir. Commercial kefir products have been designed to reduce these effects by using a pure starter culture consisting of a mixture of bacteria and yeast species that give a flavor similar to traditional kefir, but some health benefits may be …

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The Effects Of Oyster Harvest On Resident Oyster Reef Communities And Reef Structure In Coastal Louisiana Proper, Steven Lee Beck Jan 2012

The Effects Of Oyster Harvest On Resident Oyster Reef Communities And Reef Structure In Coastal Louisiana Proper, Steven Lee Beck

LSU Master's Theses

Harvest of the eastern oyster (Crassostrea virginica) is a primary contributor to oyster reef habitat disturbance in the northern Gulf of Mexico. The impacts of oyster dredging on reef substrate and resident fauna have not been thoroughly examined on the extensive sub-tidal oyster reefs of Louisiana. Several reef structure and resident community metrics were compared on unharvested and harvested reefs during the spring, summer, and fall of 2010. Unharvested reefs had higher amounts of oyster clusters, solid reef substrate, and more large oysters, while harvested reefs had higher amounts of loose shell, mixed shell/mud substrate, and elevated chlorophyll-a levels. Overall, …

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The Use Of Ionic Liquid For The Pretreatment Of Energy Cane Bagasse, Zenghui Qiu Jan 2012

The Use Of Ionic Liquid For The Pretreatment Of Energy Cane Bagasse, Zenghui Qiu

LSU Master's Theses

Lignocellulosic biomass appears to be a prospective renewable resource that can be used for the generation of biofuels and bioproducts. The major concern in lignocellulose conversion is overcoming biomass recalcitrance through pretreatment while still maintaining a green, energy efficient and cost-effective process. Energy cane is a promising energy crop with high fiber content, cold tolerance, and less fertilizer and water input requirements as compared to conventional sugarcane. Ionic liquids (ILs) are promising solvents for the pretreatment of lignocellulose as they are thermally stable, environmentally friendly, recyclable, and have low volatility. This study assessed the use of ionic liquid 1-ethyl-3-methylimidazolium acetate …

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Growth Rate And Productivity Of A Louisiana Native Microalgae/Cyanobacteria Co-Culture: Feasibility For Use In Industrial Biotechnology Applications, Athens Gomes Silaban Jan 2012

Growth Rate And Productivity Of A Louisiana Native Microalgae/Cyanobacteria Co-Culture: Feasibility For Use In Industrial Biotechnology Applications, Athens Gomes Silaban

LSU Master's Theses

Interest in biofuels research has increased in the era of uncertainty of the current fossil fuel sustainability and global climate concerns. Microalgae offer advantages over terrestrial plants as a potential source of biomass-based fuel. In this study, a Louisiana native microalgae/cyanobacteria (Chlorella vulgaris/Leptolyngbya sp.) co-culture was examined to provide foundational information of its growth conditions. Batch studies were conducted to determine optimum nitrogen and irradiance level that yields maximum co-culture biomass growth and productivity under aerated and non-aerated conditions. The optimum nitrogen level was 2.94 mM N and the optimum initial scalar irradiance range was between 400 – 800 µmol …

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Role Of Herpes Simplex Type I Glycoproteins In Entry And Cell-Cell Fusion, Sona Chowdhury Jan 2012

Role Of Herpes Simplex Type I Glycoproteins In Entry And Cell-Cell Fusion, Sona Chowdhury

LSU Doctoral Dissertations

Herpes simplex virus type I (HSV-1) is a neurotropic virus that infects primarily mucocutaneous epithelial cells and nervous tissue. Membrane fusion is an important aspect of the HSV-1 lifecycle, that occurs during viral entry (virus-cell fusion), viral spread (cell-to-cell fusion), as well as, during virion morphogenesis (assembly and egress). These membrane fusion steps involve complex interactions between multiple viral glycoproteins and cellular receptors. HSV-1 glycoprotein B (gB) is necessary but not sufficient for membrane fusion events. Despite the fact, that the majority of known hypermorphic mutations which cause extensive virus-induced cell fusion occur within glycoprotein K (gK); yet the role …

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Development Of An Estradiol-Dopamine Anagonist Protocol For Inducing Ovulation In Seasonally Anovulatory Mares, Pamela Boliew Mitcham Jan 2012

Development Of An Estradiol-Dopamine Anagonist Protocol For Inducing Ovulation In Seasonally Anovulatory Mares, Pamela Boliew Mitcham

LSU Doctoral Dissertations

Five experiments were conducted to assess potential improvements in a protocol for inducing ovulation in seasonally anovulatory mares based upon estradiol pretreatment followed by dopamine antagonist injection. The first experiment compared various doses of estradiol cypionate (ECP) and domperidone (in biodegradable microparticles), as well as additions and deletions to the protocol. It was concluded that as little as 75 mg ECP and as little as 1.5 g of domperidone could be used with success, but that both components were required. In the second experiment, timing of the injection (1, 6, or 11 days apart) and ECP dose were assessed. It …

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Evaluation Of Pleistocene To Holocene (Mis 5 To 1) Climatic Changes In Southwestern Black Sea: A Palynological Study Of Dsdp Site 380, Shannon Ferguson Jan 2012

Evaluation Of Pleistocene To Holocene (Mis 5 To 1) Climatic Changes In Southwestern Black Sea: A Palynological Study Of Dsdp Site 380, Shannon Ferguson

LSU Master's Theses

Site 380 of the Deep Sea Drilling Program [DSDP] is located in the southwestern region of the Black Sea (42°05.94’, 29°36.82’E). The location of this site is important due to its proximity to the narrow Bosphorus strait, the only present-day connection between the Black Sea and the Aegean Sea. Drops in eustatic sea level during extensive Pleistocene glacial episodes disconnected the Black Sea from the Aegean Sea at the Bosphorus strait, creating a change from brackish to freshwater within the Black Sea during these intervals. Organic-walled phytoplankton (dinoflagellate cysts, acritarchs, and fresh-water algae) are investigated to better constrain these glacio-eustatic …

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Understanding Phosphorus Dynamics Of Two Alluvial Soils Grown With Corn At Different Phosphorus Rates, Marilyn Sebial Dalen Jan 2012

Understanding Phosphorus Dynamics Of Two Alluvial Soils Grown With Corn At Different Phosphorus Rates, Marilyn Sebial Dalen

LSU Master's Theses

There is little information documented on the influence of soil properties on P availability of Louisiana alluvial soils thus this pot experiment was conducted in 2011 to: 1) evaluate the effect of P fertilizer rate on growth and development of corn grown on Perry clay and Commerce sl soils, 2) relate soil test P values using Mehlich-3 and Bray-2 procedures with yield, total biomass, and P uptake of corn, and 3) identify the soil properties that influence P partitioning into functional fractions of two alluvial soils. Different P fertilizer rates (0, 34, 67, 101 and 134 kg P2O5 ha-1) were …

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Consumer And Processor Methods To Control Salmonella And Listeria In Shrimp, Genevieve Anne Edwards Jan 2012

Consumer And Processor Methods To Control Salmonella And Listeria In Shrimp, Genevieve Anne Edwards

LSU Master's Theses

A common method used by consumers to determine if shrimp are thoroughly cooked when boiling, is to wait until the shrimp float to the top of the water and are pink in color. The purpose of this study was to determine whether the current floating and color cooking method is adequate to ensure the elimination of Listeria and Salmonella species. Furthermore, to determine if processing and storage methods reduce the effectiveness of this method. Shrimp samples were submerged into bacterial suspensions for 30 min then allowed to air dry for 1 hr and color parameters were measured using a colorimeter. …

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Biomass And Mass Balance Isotope Content Of Mussel Seep Populations, Philip Martin Riekenberg Jan 2012

Biomass And Mass Balance Isotope Content Of Mussel Seep Populations, Philip Martin Riekenberg

LSU Master's Theses

Cold seep mussels, Bathymodiolus childressi, are common cold seep constituents that form large populations at upper continental slope (500-1000 m) cold seep sites in the Northern Gulf of Mexico. These mussels utilize methane present through symbiotic relationships with methanotrophic bacteria. This study uses a coupled isotope technique to determine the relative incorporation of respiratory carbon in the shell as a measure of the availability of methane between different seep sites. This method indicates a higher abundance of methane at the Brine Pool site than at the Bush Hill site which appears significantly more resource limited and that changes in methane …

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Pmi 5011 Regulates The Ubiquitin Proteasome System In Skeletal Muscle, Heather Christianne Kirk-Ballard Jan 2012

Pmi 5011 Regulates The Ubiquitin Proteasome System In Skeletal Muscle, Heather Christianne Kirk-Ballard

LSU Doctoral Dissertations

Insulin resistance in type 2 diabetes is associated with impaired glucose and protein metabolism in skeletal muscle. The impaired insulin signaling in skeletal muscle affects muscle mass by tilting the balance between skeletal muscle protein synthesis and degradation toward degradation, a process that is primarily regulated by the ubiquitin-proteasome system. Studies have shown that an extensively characterized ethanol extract of Artemisia dracunculus L (Russian Tarragon), termed PMI 5011, enhances insulin signaling in human primary skeletal muscle cells and in a rodent model of insulin resistance. The aim of this project is to determine if the effect of PMI 5011 on …

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Dynamics Of The Sweetpotato Potyvirus Aphid Pathosystem In Louisiana, Everlyne Nafula Wosula Jan 2012

Dynamics Of The Sweetpotato Potyvirus Aphid Pathosystem In Louisiana, Everlyne Nafula Wosula

LSU Doctoral Dissertations

Sweetpotato potyviruses [Sweet potato feathery mottle virus (SPFMV), Sweet potato virus G (SPVG) and Sweet potato virus 2 (SPV2)] commonly infect sweetpotato and weedy morning glories in the USA. These viruses are transmitted in a non-persistent manner by various aphid species and cause up to 15% yield loss. Sweetpotato is vegetatively propagated, and in the USA growers are supplied with virus tested propagation material to minimize impact of viruses. However the rapid re-infection of these materials with viruses warranted further studies to determine factors that influence the epidemiology of these viruses. The objectives of this study were: (i) to determine …

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Effect Of Surfactants On The Pretreatment Of Sugarcane Bagasse With Dilute Ammonia, Shuo Cao Jan 2012

Effect Of Surfactants On The Pretreatment Of Sugarcane Bagasse With Dilute Ammonia, Shuo Cao

LSU Master's Theses

Lignocellulosic biomass is composed of cellulose, hemicellulose and lignin which are not readily available for conversion in their native form. It is widely accepted that lignin acts as the “glue” that binds cellulose and hemicellulose, giving rigidity and resistance to lignocellulose. The use of non-ionic surfactants during pretreatment can help alter the structure of lignocellulosic biomass to improve cellulose digestibility and ethanol yields. Tween 80, Tween 20, PEG 4000, or PEG 6000 was used with ammonium hydroxide for the pretreatment of sugarcane bagasse. The pretreatment was carried out by mixing sugarcane bagasse, ammonium hydroxide (28% v/v) and water at a …

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Biodiesel Production From Microalgae: Co-Location With Sugar Mills, Christian Lohrey Jan 2012

Biodiesel Production From Microalgae: Co-Location With Sugar Mills, Christian Lohrey

LSU Master's Theses

Co-location of algae production facilities with sugarcane mills in Louisiana was investigated as a way to address the bottlenecks for algal biodiesel production. Using the process modeling software Sugars™, an algal biodiesel production process was integrated with the operation of a typical-sized 10,000 metric tons/day (11,000 short tons/day) sugarcane mill to evaluate material and energy balances. A process is proposed wherein alga production is supplemented with energy, water, and CO2 available from a sugarcane mill. The Energy Return on Invested, EROI (a ratio of the energy produced/energy required) of the proposed algal biodiesel production process was 1.25; meaning 25% more …

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Vitamin D Status, Adiposity, And Athletic Performance Measures In College-Aged Students, Laura Forney Jan 2012

Vitamin D Status, Adiposity, And Athletic Performance Measures In College-Aged Students, Laura Forney

LSU Master's Theses

Research suggests that elevated serum concentrations of 25-hydroxyvitamin D (25OHD) are associated with slowing the development of many age-related chronic diseases. Recent reports also show a positive relationship between 25OHD and muscle synthesis, strength, power and decreased body fat in elderly individuals. However, these findings have not been consistently reported in younger, healthy populations. PURPOSE: To investigate the relationship between 25OHD levels, body composition, measures of aerobic fitness and muscular strength and power in a young, physically active population. METHODS: Thirty-nine active subjects (20 males, 19 females; 23 ± 0.7 years old) reported to the lab six times for testing. …

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Improving Nitrogen Management In Sugarcane Production Of The Mid-South Using Remote Sensing Technology, Joshua Lofton Jan 2012

Improving Nitrogen Management In Sugarcane Production Of The Mid-South Using Remote Sensing Technology, Joshua Lofton

LSU Doctoral Dissertations

In Louisiana, current N rate recommendations for sugarcane production are based on multiple year N response trials and refined based on soil and crop variables. Without accounting for current growing conditions, recommendations can potentially lead to over- or under-application of N. The objectives of this research were to 1) determine the ability of an in-season response index value (RINDVI) to estimate sugarcane yield response index (RIHarvest), 2) determine if sugarcane yield potential could be determined using normalized difference vegetative index (NDVI), and 3) estimate the optimum N rate and application timing for sugarcane production in Louisiana. Experiments were established in …

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Anatomical Studies Of Canine Vascular And Ligamentous Ear Structures With Revelance To Acute-Onset Deafness, Cathryn Kay Stevens-Sparks Jan 2012

Anatomical Studies Of Canine Vascular And Ligamentous Ear Structures With Revelance To Acute-Onset Deafness, Cathryn Kay Stevens-Sparks

LSU Doctoral Dissertations

Hearing loss in dogs and cats following dental or ear procedures performed under anesthesia has recently been reported. The most likely causes for this acute-onset deafness were considered to be mechanical or vascular. Jaw manipulation as a possible cause of acute-onset deafness in the dog was investigated in the current study. Structures adjacent to the temporomandibular joint were of interest because changes in jaw orientation could disrupt vessels and nerves in this area. Current descriptions of the anatomy of the vasculature supplying the canine ear are either incomplete or inconsistent. Another considered cause was a jaw-ear connection via a ligamentous …

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Effects Of Two Different Behavioral Swallowing Exercise Protocols Performed During Radiation Therapy On Swallowing Physiology, Function, Quality-Of-Life And Weight Outcomes Following Organ-Preservation Treatments For Head And Neck Cancers, Aneesha Virani Jan 2012

Effects Of Two Different Behavioral Swallowing Exercise Protocols Performed During Radiation Therapy On Swallowing Physiology, Function, Quality-Of-Life And Weight Outcomes Following Organ-Preservation Treatments For Head And Neck Cancers, Aneesha Virani

LSU Doctoral Dissertations

Objective: The purpose of this study was to investigate the effects of two different prophylactic behavioral swallowing exercise regimens performed by head and neck cancer/s (HNC) patients during radiation therapy with/without chemotherapy (RT/C) on swallowing physiology, function, quality-of-life (QOL) and weight outcomes at the completion of RT/C. Feeding tube (PEG) status at 3 months post-treatment was also compared. Methods: This study was conducted via a prospective design. 50 patients diagnosed with HNC who were to undergo RT/C were recruited at pre-treatment based on the inclusion criteria: functional swallowing abilities; without prophylactic PEG tubes; ability to comprehend and perform therapy tasks …

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En(Gendering) Policy: Gender Policies In Former Soviet Republics, Natasha Lachelle Bingham Jan 2012

En(Gendering) Policy: Gender Policies In Former Soviet Republics, Natasha Lachelle Bingham

LSU Doctoral Dissertations

This dissertation examines gender policies in former Soviet republics. Gender policies are depicted as traditional policies (including such policies as child support, spousal support, and inheritance rights), violence against women policies (domestic violence, marital rape, sexual assault, and sexual harassment), and human trafficking policies. It builds upon previous works with a multi-methodological approach (content analysis, logistical regression, and qualitative survey analysis). The focus here is on the factors that influence adoption of formal policies in former Soviet republics from 1993-2008. I explore variance across both countries and policy areas, assessing whether (and why) certain post-Soviet states are more likely to …

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Measurement Of Pain, Pain Disability, And Pain Beliefs Of Morbidly Obese Adults, Brooke Louise Barbera Jan 2012

Measurement Of Pain, Pain Disability, And Pain Beliefs Of Morbidly Obese Adults, Brooke Louise Barbera

LSU Doctoral Dissertations

Both obesity and pain are pervasive public health problems, contributing to significant disability in the United States and worldwide. Studies have demonstrated a positive association between obesity and pain, with increasing BMI related to increasing levels of pain intensity; however, the mechanisms underlying this relationship are not well understood. The present study assessed the presence of pain, pain-related disability, and pain beliefs in a morbidly obese (BMI > 40 kg/m2) sample participating in a medically-supervised, nonsurgical weight loss intervention. After controlling for demographic variables and depression, pain was reported in 36.7% of the sample (N = 390). Results indicated that greater …

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Influences On Behavior Adaptations To Reduce Exposure To Environmental Hazards Among Residents Of Louisiana's Upper Industrial Corridor, Corrinthia Marie Hinton Jan 2012

Influences On Behavior Adaptations To Reduce Exposure To Environmental Hazards Among Residents Of Louisiana's Upper Industrial Corridor, Corrinthia Marie Hinton

LSU Master's Theses

Over the past century, the growth in petrochemical manufacturing within Louisiana’s Upper Industrial Corridor brought economic development, but also introduced toxic emissions and environmental exposure risks to residents of the area. For the citizens living in close proximity to multiple facilities there is the added risk of chemical exposure from environmentally hazardous accidents. This study seeks to gain insights into patterns of risk-reducing behaviors of residents in East Baton Rouge Parish so that better educational outreach programs can be developed. This research addresses the following questions: To what extent are residents of Baton Rouge taking steps to reduce environmental exposure …

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Pregnancy And The Relationship To Age-Related Macular Degeneration, Ann Hardin Shaw Jan 2012

Pregnancy And The Relationship To Age-Related Macular Degeneration, Ann Hardin Shaw

LSU Master's Theses

The Macular Study was a case control study that evaluated if parity and other participant characteristics predicted the diagnosis of age-related macular degeneration (AMD). Women, compared to men, are at higher risk for AMD. AMD is one of the leading causes of blindness in the elderly population [1]. Docosahexaenoic acid (DHA, 22:6n-3) is a long-chain fatty acid that is essential for the structure and function of the eye. During pregnancy the growing fetus depletes the maternal stores of DHA through placental transfer. The fetus needs an ample supply of DHA for proper retinal and central nervous system development. To date …

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Investigating Children's Social Support Systems: Comparison Of Interviews And Interviews With Pictures, Leah Kathrine Hebert Jan 2012

Investigating Children's Social Support Systems: Comparison Of Interviews And Interviews With Pictures, Leah Kathrine Hebert

LSU Master's Theses

The present research study seeks to improve upon prior research on child social supports by using an already existing eco-map protocol to inform two interviews on child social supports. This study will help further develop an assessment for understanding child social supports and social networks. This project examines the similarities and differences between child reports of social support using: a) child interview-only protocol and b) interview-with-pictures protocol. The two main research questions include: 1) Do children report the same number of social supports using the child interview-only protocol as the interview-with-pictures protocol? and 2) Do children report the same kind …

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Reduced Protein For Late-Lactation Dairy Cows, Andre De Barros Duarte Pereira Jan 2012

Reduced Protein For Late-Lactation Dairy Cows, Andre De Barros Duarte Pereira

LSU Master's Theses

Excess protein in dairy cattle diets unnecessarily increases the cost of production and may contribute to environmental pollution. The objective of this research was to evaluate the effect of feeding dairy cows with two levels of dietary protein on animal performance and manure characteristics. Two experiments were carried out with 24 lactating dairy cows each. Experiment 1 was corn silage based and had a control TMR (HP1) estimated to contain 16.5% CP with SBM and treatment TMR (LP1; 13.5% CP) using DDGS and rumen protected Lys and Met. Experiment 2 was ryegrass haylage based and had a control TMR (HP2) …

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