Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

Louisiana State University

Discipline
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 901 - 930 of 8029

Full-Text Articles in Entire DC Network

"I'M Not Your Ride Or Die": A Qualitative Study Of Black Women's Experiences In Intimate Marketplaces, Alana Jamelle Peck Aug 2022

"I'M Not Your Ride Or Die": A Qualitative Study Of Black Women's Experiences In Intimate Marketplaces, Alana Jamelle Peck

LSU Doctoral Dissertations

Black women’s marriage rates have consistently been on the decline since the 1960’s and currently, Black women are the least likely to get married across all races and genders (Bureau 2020). Scholars have been using these statistics to tell monolithic narratives of Black womanhood, and even blame Black women for larger issues within the Black community (Collins 2000, Johnson and Loscocco 2015; Moynihan 1965). Prior scholars have also attempted to provide solutions to this problem, while never even approaching Black women, to suggest ways that they can increase their marriage rates and consequently uplift Black families and the Black community …


Improved Building-Specific Flood Risk Assessment And Implications Of Depth-Damage Function Selection, Ehab Gnan, Carol J. Friedland, Md Adilur Rahim, Rubayet Bin Mostafiz, Robert V. Rohli, Fatemeh Orooji, Arash Taghinezhad, Jasmin Mcelwee Aug 2022

Improved Building-Specific Flood Risk Assessment And Implications Of Depth-Damage Function Selection, Ehab Gnan, Carol J. Friedland, Md Adilur Rahim, Rubayet Bin Mostafiz, Robert V. Rohli, Fatemeh Orooji, Arash Taghinezhad, Jasmin Mcelwee

Faculty Publications

Average annual loss (AAL) is traditionally used as the basis of assessing flood risk and evaluating risk mitigation measures. This research presents an improved implementation to estimate building-specific AAL, with the flood hazard of a building represented by the Gumbel extreme value distribution. AAL is then calculated by integrating the area under the overall loss-exceedance probability curve using trapezoidal Riemann sums. This implementation is compared with existing AAL estimations from flood risk assessment. A sensitivity analysis is conducted to examine the variability in AAL results based on depth-damage function (DDF) choice. To demonstrate the methodology, a one-story single-family residence is …


Covid-19 In The United States As Affective Frame, John Protevi Aug 2022

Covid-19 In The United States As Affective Frame, John Protevi

Faculty Publications

In this paper I attempt to contribute to the developing field of “political philosophy of mind.” To render concrete the notion of “affective frame,” a social situation which pre-selects for salience and valence of environmental factors relative to a subject’s life, I conduct a case study of a deleterious socially instituted affective frame, which, during the early days of the COVID19 pandemic in the United States, produced individuated circumstances that came crashing down on “essential workers” who were forced into a double bind. We saw here an untenable and ultimately fatal situation that forced a choice between, on the one …


The Association Of Cuzd1 And Stat5 In Adipocytes, Zachary Wigger Aug 2022

The Association Of Cuzd1 And Stat5 In Adipocytes, Zachary Wigger

LSU Master's Theses

Obesity is a metabolic disorder that has turned into a global epidemic over the past few decades. Adipocyte dysfunction brought on by obesity can lead to increased risk of metabolic diseases such as cardiovascular disease and type 2 diabetes mellitus (T2DM). Knowing how adipocytes function to regulate metabolic homeostasis is critical. CUZD1 (CUB and Zona Pellucida-like Domain-containing protein 1) has not been studied in the context of adipocyte biology but could play a role in several metabolic processes. CUZD1 has been suggested to be a biomarker for several inflammatory diseases and cancers but little functional data has been published. There …


Ultraviolet Chiral Plasmonics, Tiago Ramos Leite Da Silva Aug 2022

Ultraviolet Chiral Plasmonics, Tiago Ramos Leite Da Silva

LSU Doctoral Dissertations

The far- (λ < 250 nm) ultraviolet (UV) chiroptical response from protein secondary structures can aid in understanding protein interactions for drug discovery. Chiral metasurfaces have attracted attention to enhance the intrinsically weak biomolecular optical activity for improved structural biology characterization. However, due to materials and fabrication challenges, current metasurfaces mostly operate in the visible and near-infrared regimes with limited, if any, response in far-UV. The large spectral mismatch between metasurface and biomolecular resonances limits the chiral enhancement factor. In this work, we study aluminum planar metamaterials which display optical activity in the UV region. In the first part of this work, we experimentally and computationally investigate the resonant plasmonic-biomolecular interactions in the far-UV. To the best of our knowledge, our work is the first to experimentally explore these resonant interactions in the biologically relevant far-UV regime. We demonstrate experimentally and computationally enhanced chiral sensing of sub-10 nm amino acid films. We develop a full-wave computational model of a biomolecular film on an aluminum gammadion array using experimentally derived chiral parameters (κ) for L- and DTyrosine films. Our model shows that detectable enhancements in the circular dichroism (CD) and birefringence (CB) signals from small amounts of biomolecules are only possible when tight spectral overlap exists between the plasmonic and biomolecular chiroptical signals. We support this conclusion experimentally by fabricating aluminum gammadion arrays on fused silica substrates and sublimating ultrathin racemic, L- and D- Tyrosine films on the arrays. Complete Mueller matrix analysis of the resonant plasmonic-biomolecular system agrees with our computational results and demonstrates the importance of using far-UV active metasurfaces for enhancing natural optical activity. In the second part of this work, we study hybrid aluminum reflective metasurfaces, which leverage both local and propagating surface plasmons (PSP). These materials display strong bi-anisotropy with rich polarization conversion properties. We explore several geometries and verify how their symmetry properties affect their far-field response. Our observations provide an opportunity for researchers to improve the chiral response of plasmonic metasurfaces, especially in the challenging ultraviolet regime.


Developing A Conceptual Passive Contact Tracing System For Commercial Buildings Using Wifi Indoor Positioning, Sorena Vosoughkhosravi, Amirhosein Jafari Aug 2022

Developing A Conceptual Passive Contact Tracing System For Commercial Buildings Using Wifi Indoor Positioning, Sorena Vosoughkhosravi, Amirhosein Jafari

Faculty Publications

Contact tracing is one of the critical tools for fighting against pandemic disease outbreaks, such as the fast-growing SARS-CoV-2 virus and its different variants. At present, automated contact tracing systems face two main challenges: (1) requiring application installation on smart devices and (2) protecting the users’ privacy. This study introduces a conceptual passive contact tracing system using indoor WiFi positioning to address these challenges and investigate the role of such a system in commercial buildings. In this regard, this study uses a simulated small-office layout in a case study to demonstrate the applicability of the proposed system. The special use …


The Need For A Framework Addressing The Temporal Aspects Of Fish Sperm Motility Leading To Community-Level Standardization, Harvey Blackburn, Leticia Torres, Yue Liu, Terrence R. Tiersch Aug 2022

The Need For A Framework Addressing The Temporal Aspects Of Fish Sperm Motility Leading To Community-Level Standardization, Harvey Blackburn, Leticia Torres, Yue Liu, Terrence R. Tiersch

Faculty Publications

Motility is a widely available parameter that can be used to assess sperm quality of aquatic species. Sperm from fishes with external fertilization usually undergo a dynamic and short-lived period of motility after activation. The common practice of assigning a single value at an arbitrary peak of motility presents challenges for reproducibility, community-level standardization, and comparisons across studies. This study aimed to explore statistical approaches to standardize motility reporting, and to develop an initial framework for community-level standards. Sperm samples from 14 zebrafish (Danio rerio) with a total of 21,705 cells were analyzed by use of computer-assisted sperm analysis with …


The Curve Not Taken: Effects Of Covid-19 International Comparison News, Seon Woo Kim, Martina Santia, Raymond J. Pingree, Ayla Oden, Kirill Bryanov, Jessica Wyers Aug 2022

The Curve Not Taken: Effects Of Covid-19 International Comparison News, Seon Woo Kim, Martina Santia, Raymond J. Pingree, Ayla Oden, Kirill Bryanov, Jessica Wyers

Faculty Publications

International news can inform people not only about what is happening in other countries, but also about how their own country could benefit from policies that have proved successful elsewhere. Specifically, international policy comparison news, or news that compares the policies of two or more countries on the same issue, is a potentially important but underutilized and understudied form of news content. We use an experiment to test effects of exposure to news comparing the COVID-19 pandemic policies of the U.S. versus South Korea, and find that this increases knowledge of policy differences between the two countries, support for adopting …


Bio-Inspired Geotechnical Engineering: Principles, Current Work, Opportunities And Challenges, Alejandro Martinez, Jason Dejong, Idil Akin, Ali Aleali, Chloe Arson, Jared Atkinson, Paola Bandini, Tugce Baser, Rodrigo Borela, Ross Boulanger, Matthew Burrall, Yuyan Chen, Clint Collins, Douglas Cortes, Sheng Dai, Theodore Dejong, Emanuela Del Dottore, Kelly Dorgan, Richard Fragaszy, J. David Frost, Robert Full, Majid Ghayoomi, Daniel I. Goldman, Nicholas Gravish, Ivan L. Guzman, James Hambleton, Elliot Hawkes, Michael Helms, David Hu, Lin Huang, Sichuan Huang, Christopher Hunt, Duncan Irschick Aug 2022

Bio-Inspired Geotechnical Engineering: Principles, Current Work, Opportunities And Challenges, Alejandro Martinez, Jason Dejong, Idil Akin, Ali Aleali, Chloe Arson, Jared Atkinson, Paola Bandini, Tugce Baser, Rodrigo Borela, Ross Boulanger, Matthew Burrall, Yuyan Chen, Clint Collins, Douglas Cortes, Sheng Dai, Theodore Dejong, Emanuela Del Dottore, Kelly Dorgan, Richard Fragaszy, J. David Frost, Robert Full, Majid Ghayoomi, Daniel I. Goldman, Nicholas Gravish, Ivan L. Guzman, James Hambleton, Elliot Hawkes, Michael Helms, David Hu, Lin Huang, Sichuan Huang, Christopher Hunt, Duncan Irschick

Faculty Publications

A broad diversity of biological organisms and systems interact with soil in ways that facilitate their growth and survival. These interactions are made possible by strategies that enable organisms to accomplish functions that can be analogous to those required in geotechnical engineering systems. Examples include anchorage in soft and weak ground, penetration into hard and stiff subsurface materials and movement in loose sand. Since the biological strategies have been 'vetted' by the process of natural selection, and the functions they accomplish are governed by the same physical laws in both the natural and engineered environments, they represent a unique source …


The Thermodynamics Of A Stochastic Geometry Model With Applications To Non-Extensive Statistics, O.K. Kazemi, A. Pourdarvish, J. Sadeghi Jul 2022

The Thermodynamics Of A Stochastic Geometry Model With Applications To Non-Extensive Statistics, O.K. Kazemi, A. Pourdarvish, J. Sadeghi

Journal of Stochastic Analysis

No abstract provided.


Creating A Public Space For Georeferencing Sanborn Maps: A Louisiana Case Study, Adam Cox Jul 2022

Creating A Public Space For Georeferencing Sanborn Maps: A Louisiana Case Study, Adam Cox

LSU Master's Theses

As institutional archives digitize their historical map collections and make them publicly available online, new methods for engaging with these materials emerge. Georeferencing the maps transforms their content from static images to dynamic map overlays, and allows for the extraction of geographic data like building footprints or place name coordinates. Many organizations have turned to crowdsourcing to georeference their large holdings, and this thesis approaches crowdsourced georeferencing from the perspective of participatory heritage, taking much inspiration from the idea of the archival commons. To test these ideas, a new extension was created for GeoNode—an open source geospatial content management system—that …


Understanding The Effects Of Socioeconomic Variables, Career Motivation, And Msw Education On Practice Preferences: A Quantitative National Survey Of Msw Students, Tina B. Granger Jul 2022

Understanding The Effects Of Socioeconomic Variables, Career Motivation, And Msw Education On Practice Preferences: A Quantitative National Survey Of Msw Students, Tina B. Granger

LSU Doctoral Dissertations

Over the past several decades, there has been much debate over the role of social workers in the mental health field. Factors related to career motivation and practice preferences has had limited research and many have attributed materialistic and self-seeking motives as the reason for a social work career choice. Research has shown that there may be a divergence or ‘mission drift’ from traditional social work values and mission of social justice and social change at the systems level. Identifying factors related to career motivation and practice preferences is important in an ever-evolving field such as social work. This study …


“Not Nearly What It Used To Be”: A Mixed Methods Study On Hazing And Organizational Culture In Historically White Fraternities, Kimberly Rogers Davis Jul 2022

“Not Nearly What It Used To Be”: A Mixed Methods Study On Hazing And Organizational Culture In Historically White Fraternities, Kimberly Rogers Davis

LSU Doctoral Dissertations

Hazing is a significant concern on college campuses, especially as students continue to die following hazing incidents in student organizations. Fraternities, particularly historically White fraternities (HWFs), have been the site of many recent hazing deaths. However, fraternity and sorority life leaders and advocates often argue that the chapters where hazing tragedies occur are the minority in a broader system that offers numerous contributions to society. Rather than a fraternity or sorority problem, hazing may result from cultural influences within and around the organization. Thus, the purpose of this study is to understand how organizational culture, such as chapter culture and …


The Link Between Intracellular Calcium Signaling And The Nitric Oxide-Dependent Release Of Chloride In Retinal Amacrine Cells, Li Zhong Jul 2022

The Link Between Intracellular Calcium Signaling And The Nitric Oxide-Dependent Release Of Chloride In Retinal Amacrine Cells, Li Zhong

LSU Doctoral Dissertations

Regulation of cytosolic Cl- is an important determinant in synaptic function. Our lab has discovered that cytosolic Cl- is regulated not only by transport across the plasma membrane but that Cl- can also be released from internal Cl- stores (Hoffpauir et al., 2006) in amacrine cells (ACs). Nitric oxide (NO) is a small molecule neurotransmitter that has been demonstrated to be involved in cytosolic Ca2+ elevations and Cl- regulation via activation of the cystic fibrosis transmembrane regulator (CFTR) (Krishnan et al., 2017). Here, I test the hypothesis that intracellular Ca2+ signaling mediates the …


4d Spatio-Temporal Electric Field Characterization Of Ultrashort Light Pulses Undergoing Filamentation, Lana Neoričić, Christoph Jusko, Sara Mikaelsson, Chen Guo, Miguel Miranda, Shiyang Zhong, Felix Garmirian, Balazs Major, Jeffrey M. Brown, Mette B. Gaarde, Arnaud Couairon, Uwe Morgner, Milutin Kovačev, Cord L. Arnold Jul 2022

4d Spatio-Temporal Electric Field Characterization Of Ultrashort Light Pulses Undergoing Filamentation, Lana Neoričić, Christoph Jusko, Sara Mikaelsson, Chen Guo, Miguel Miranda, Shiyang Zhong, Felix Garmirian, Balazs Major, Jeffrey M. Brown, Mette B. Gaarde, Arnaud Couairon, Uwe Morgner, Milutin Kovačev, Cord L. Arnold

Faculty Publications

We present an experimental method capable of capturing the complete spatiotemporal dynamics of filamenting ultrashort laser pulses. By employing spatially resolved Fourier transform spectrometry in combination with the capability to terminate the filament at any length, we can follow the nonlinear dynamics in four dimensions, i.e. the transverse domain, time and filament length. Our method thus not only enables the full characterization of the filamentation process throughout its evolution, but also allows to identify and select laser pulses with desired parameters.


Gis Automated Delineation And Analysis Of Cancer Service Areas In The United States, Changzhen Wang Jul 2022

Gis Automated Delineation And Analysis Of Cancer Service Areas In The United States, Changzhen Wang

LSU Doctoral Dissertations

Delineating a meaningful and reliable geographic unit pertaining to cancer care is essential in examining geographic variations in cancer care for better analysis, management, and planning. Public health researchers have devoted great efforts to defining various service areas, which; however, lack scientific rigor or are unrepresentative of the highly specialized cancer care markets, and their methods have become obsolete in the era of big data and high geo-computation. This study develops the “Spatially Constrained Leiden (ScLeiden)” method, a network community detection algorithm in Geographic Information Systems (GIS), and applies it to delineate a series of coherent cancer service …


Determining The 3d Structure And Dynamics Of Mouse Notch1 Epidermal Growth Factor-Like Repeat 26 Using Nuclear Magnetic Resonance Spectroscopy, Kendra D. Jenkins Jul 2022

Determining The 3d Structure And Dynamics Of Mouse Notch1 Epidermal Growth Factor-Like Repeat 26 Using Nuclear Magnetic Resonance Spectroscopy, Kendra D. Jenkins

LSU Doctoral Dissertations

NOTCH is a transmembrane protein that transmits signals between cells in direct contact and alters gene expression. The signaling pathway is important because it is involved in cell fate determination. The NOTCH1 extracellular domain has 36 epidermal growth factor-like (EGF) repeats that are responsible for ligand binding. Mutations in the EGF-like repeats cause various neurological and developmental disorders. NOTCH1 signaling initiates when DSL ligands bind to EGF11-12, the minimum Ligand Binding Domain (LBD). The Abruptex region of the NOTCH1 protein (EGF24-29) is a highly glycosylated, flexible region with calcium binding sites that regulates NOTCH1 signaling and binding by interacting with …


Winding Down River Road, Gillian Harper Jul 2022

Winding Down River Road, Gillian Harper

LSU Master's Theses

As a mechanism to explore my temporary home in Louisiana, Winding Down River Road is a collection of artworks that integrates natural materials collected from landscapes in southern Louisiana with steel and petroleum-based products. My interest in researching environmental issues, ecology, and industry has shaped my vehicles for observation and how I generate data. Through a variety of methodologies, I am considering how climate change is forcing many of us to re-contextualize how our home can be affected by the very industries we rely on. Personal engagement with residents living in the dystopian atmosphere of southern Louisiana’s industrial corridor and …


Developmental Competence Of Oocytes Derived From Seasonally Anovulatory Mares Treated With Estradiol And Sulpiride, Michelle O. Vetter Jul 2022

Developmental Competence Of Oocytes Derived From Seasonally Anovulatory Mares Treated With Estradiol And Sulpiride, Michelle O. Vetter

LSU Master's Theses

Over the course of two years (2021-2022), two experiments were conducted during the nonbreeding seasons to evaluate the in vitro developmental potential of oocytes derived from seasonally anovulatory mares treated with ECP-sulpiride. The objective of experiments 1 and 2 were to compare pooled recovery and maturation rates of oocytes from mares treated with estradiol cypionate (ECP) and sulpiride with oocytes collected from naturally transitional mares (controls). Cleavage rates of oocytes subjected to intracytoplasmic sperm injection (ICSI) were compared only in experiment 2. In experiments 1 and 2, a total of 36 light horse and pony-cross anovulatory mares were used. Treatment …


Aphid Herbivory On Industrial Hemp, Cannabis Sativa L., And The Impact Of Plant-Soil Feedbacks On Hemp Performance In Louisiana, Alex Leede Jul 2022

Aphid Herbivory On Industrial Hemp, Cannabis Sativa L., And The Impact Of Plant-Soil Feedbacks On Hemp Performance In Louisiana, Alex Leede

LSU Master's Theses

Industrial hemp, Cannabis sativa L., is defined as having less than 0.3% delta-9-tetrahydrocannabinol by dry weight. It can be grown for fiber, food, oil, and cannabinoids. As a newly legalized crop, little research exists regarding the cultivation or pest management practices in Louisiana. The versatility of industrial hemp may allow for substantial future production in Louisiana. This project set out to understand the impact of previous crops on hemp performance and soil microbial communities. Soybean, Glycine max L. (Merr), grain sorghum, Sorghum bicolor (L.) Moench, and sweetpotato, Ipomeae batatas (L.) Lam, were selected as soil conditioning crops because they are …


Footprint Of Farm To School Programs In Louisiana, Whitney R. Mckinzie Jul 2022

Footprint Of Farm To School Programs In Louisiana, Whitney R. Mckinzie

LSU Master's Theses

The farm to school (F2S) program is an initiative participated in by schools with the intent to promote healthy lifestyle choices among children and introduce agricultural lessons into school curriculums. F2S programs have gained popularity since their introduction in 1996. In Louisiana, there were 59 programs in 13 public school districts implemented throughout the state in 2013. A large increase was seen in 2015, as there were 152 programs in 13 public school districts in 2015, and again in 2019 with 606 programs in 37 public school districts. The program has been active for over 20 years, however, there has …


Parasocial Relationships With Online Influencers, Sydney Reynolds Jul 2022

Parasocial Relationships With Online Influencers, Sydney Reynolds

LSU Master's Theses

Current research on parasocial relationships has a focus on the media consumer and the media personae in television or film where the media consumer exerts time and emotional energy into the relationship while the character is unaware of their existence. The purpose of this study is to explore the Influencers and further conceptualize the term parasocial relationship to include these online mediated relationships and interactions. This study explores parasocial relationships and what is associated with them in the context of social media with respondents who consistently engage with social media Influencers. Social media Influencers address their audiences personally and share …


Development And Validation Of A Measure For Social Support: Perceived Social Support Inventory, Lindsay Marie Clark Jul 2022

Development And Validation Of A Measure For Social Support: Perceived Social Support Inventory, Lindsay Marie Clark

LSU Doctoral Dissertations

Abstract

The literature consistently acknowledges the protective function of social support against various negative psychological and physiological outcomes (Cohen & Wills, 1985; Dunst et al., 1986; Taylor, 2011; Uchino, 2009). Further, social support can emerge from different members that comprise an individual’s social network, such as friends, family, teachers, colleagues, and members of common interest groups. Given the important role that social support plays in health outcomes, the importance of psychometrically sound measures for assessing the construct is essential for use in research and clinical settings. However, many of the current measures of social support are outdated, are limited in …


Toybox: A New Approach For Auxiliary Percussion Instruments At The Beginning Level, Spencer Craig Jones Jul 2022

Toybox: A New Approach For Auxiliary Percussion Instruments At The Beginning Level, Spencer Craig Jones

LSU Doctoral Dissertations

Currently, there exist little materials to introduce elementary students to auxiliary percussion instruments. There is a need for the creation of more instructional material for this age group that provides pedagogy descriptions for the instruments as well as playing examples. The purpose of this project is to create a method book that provides pedagogy examples for auxiliary percussion instruments while also providing playing examples to use.


Screening Soybean Cultivars For Resistance To Aerial Blight Caused By Rhizoctonia Solani Ag1-Ia, Kensy D. Rodriguez-Herrera Jul 2022

Screening Soybean Cultivars For Resistance To Aerial Blight Caused By Rhizoctonia Solani Ag1-Ia, Kensy D. Rodriguez-Herrera

LSU Master's Theses

Aerial blight, caused by the fungus Rhizoctonia solani AG1-IA, is an economically important soybean disease in the mid-South. The same pathogen also infects other crops commonly rotated with soybeans like rice, corn, and grain sorghum. Rhizoctonia solani AG1-IA is difficult to manage because of its soilborne nature and the production of long-term survival structures, sclerotia. Management has relied on fungicide applications during the season. Still, there is an increasing prevalence of resistance to commonly used strobilurin fungicides and an urgent need to identify soybean cultivars resistant to aerial blight. Since the patchy distribution of the pathogen complicates field cultivar screening, …


Does Well-Being Moderate The Association Of Harm Avoidance With Cognitive Decline, Christopher Reed Jul 2022

Does Well-Being Moderate The Association Of Harm Avoidance With Cognitive Decline, Christopher Reed

LSU Master's Theses

The Dual-Factor Model of mental health highlights the need to consider both constructs associated with psychopathology and subjective well-being in relation to clinical outcomes. Harm avoidance, which is associated with behavioral inhibition, has been negatively associated with cognitive change. While subjective well-being has been positively associated with cognition. The current study aimed to examine both constructs in a single model to examine well-being as a moderator of the association of harm avoidance with cognitive decline. A sample of 728 older adults from the Rush University Memory and Aging Project was used. Participants completed batteries of questionnaires and neuropsychological tests at …


The Relationship Between Family Environment And Specific Phobias, Jerrica Renee Guidry Jul 2022

The Relationship Between Family Environment And Specific Phobias, Jerrica Renee Guidry

LSU Doctoral Dissertations

Specific phobias are the most prevalent disorder among adults and highly common among children. Additionally, specific phobias diagnosed in childhood has been associated other serious mental illnesses later in life (de Vrie et al., 2019). Research pertaining to parenting factors associated with anxiety disorders has been extensive, including parental psychopathology, parent-child relationships, parental control, and parental stress. However, less emphasis has been placed on familial factors which may contribute to the development and maintenance of specific phobias. According to the limited literature focusing on the family factors associated with specific phobias, parent psychopathology and parent over-control are important factors. Multiple …


Scale Up Of A Novel Method To Maximize Malonyl-Coa In Escherichia Coli, Clifford Harris Leblanc Iv Jul 2022

Scale Up Of A Novel Method To Maximize Malonyl-Coa In Escherichia Coli, Clifford Harris Leblanc Iv

LSU Master's Theses

The instability of oil’s price along with its limited availability and impact on the environment motivate the search for an alternative feedstock that can sustain the profitability of chemical companies. Industrial biotechnology can promote renewable sources of energy and products by using microorganisms to produce a wide range of chemical compounds. The three-carbon metabolite, malonyl-CoA, can serve as a precursor to a variety of industrial chemicals. The major hurdle with using malonyl-CoA in industry is that its intracellular concentration in Escherichia Coli is very low. Previous attempts to increase the intracellular level of malonyl-CoA have ranged from genetic engineering of …


Micro Heat Exchanger To Cool Cerebrospinal Fluid For Brain Injury Treatment, Sachin Dahiya Jul 2022

Micro Heat Exchanger To Cool Cerebrospinal Fluid For Brain Injury Treatment, Sachin Dahiya

LSU Master's Theses

Hypothermia is accepted as a method to preserve cells and tissue. Clinical evidence shows that administration of hypothermia could lead to neuroprotection after cardiac arrest. Non-invasive methods such as surface cooling devices, drugs and cold liquid ventilation are available to induce hypothermia. These approaches for achieving hypothermia have not been optimized yet. The surface cooling methods are generally slow and may lead to additional thermal shock to the body. Here, we propose a rapid, selective cooling method for the brain using a micro heat exchanger to cool the cerebrospinal fluids (CSF). We designed and 3D printed a U-type heat exchanger …


The Importance Of Co2 Recapture In The Co2 Concentrating Mechanism Of Chlamydomonas Reinhardtii, Ashwani Rai Jul 2022

The Importance Of Co2 Recapture In The Co2 Concentrating Mechanism Of Chlamydomonas Reinhardtii, Ashwani Rai

LSU Doctoral Dissertations

The aim of this thesis is to investigate the CO2 concentrating mechanism (CCM) of Chlamydomonas reinhardtii and to develop a quick method for estimating the activity of carbonic anhydrases (CAs). The first project demonstrates that there are two almost identical mitochondrial CAs in C. reinhardtii, CAH4 and CAH5, that help to maintain photosynthesis and minimize the leak of CO2 generated by respiration and photorespiration. We used an RNAi approach to reduce the expression of CAH4 and CAH5 so that their physiological functions could be studied. RNAi mutants with low expression of CAH4 and CAH5 have impaired rates …